AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0393340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0393340 MtrunA17_Chr3g0078561 89.409 406 18 1 1 381 1 406 0.0 720
Msa0393340 MtrunA17_Chr8g0356011 37.071 437 189 17 1 377 1 411 5.50e-49 170
Msa0393340 MtrunA17_Chr5g0440311 36.735 98 60 2 11 108 4 99 2.16e-13 69.7
Msa0393340 MtrunA17_Chr5g0440291 30.822 146 76 6 19 154 13 143 5.32e-13 68.6
Msa0393340 MtrunA17_Chr3g0078491 27.340 406 234 18 20 380 9 398 7.58e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0393340 AT5G04890.1 44.944 89 48 1 20 108 16 103 1.35e-19 89.7
Msa0393340 AT2G27140.1 32.787 122 79 3 17 138 11 129 9.41e-14 70.5
Msa0393340 AT3G10680.1 32.692 104 69 1 4 107 7 109 1.46e-13 72.4

Find 78 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTTCAACTTGTGATTTA+AGG 0.135801 3_2:+7434096 None:intergenic
AAAGCCTTTAGTTGCAAATA+TGG 0.210552 3_2:-7433382 Msa0393340:CDS
GCTAGAAGAGATAAACTTAT+AGG 0.216530 3_2:+7433317 None:intergenic
CTTCAAGGCAAGTTTGAAAA+TGG 0.223575 3_2:-7434155 Msa0393340:CDS
AAAGTTTCATACACAGGATT+TGG 0.240469 3_2:+7435097 None:intergenic
TTGGTTCCCTTGGGTGTTCT+AGG 0.258214 3_2:+7434050 None:intergenic
ACCTTCTTGTCTCTAATTAT+AGG 0.272544 3_2:+7433990 None:intergenic
CTCCATTAATCCTTAGTGTT+CGG 0.276081 3_2:+7434258 None:intergenic
CCCTTGGGTGTTCTAGGACT+TGG 0.288101 3_2:+7434056 None:intergenic
GAATAAATCAAGAAGCAAAA+AGG 0.292738 3_2:-7435168 Msa0393340:five_prime_UTR
TCCATTAATCCTTAGTGTTC+GGG 0.297124 3_2:+7434259 None:intergenic
TTTCTTCACATTCACCTTCC+TGG 0.298848 3_2:-7435034 Msa0393340:intron
ATCAAAAGGAGAAGAATATT+TGG 0.299073 3_2:-7433568 Msa0393340:CDS
TTAGCAATAAATAAAATAAA+TGG 0.300207 3_2:+7433197 None:intergenic
CAGAATATGATTGGTCAAAT+AGG 0.302570 3_2:+7434204 None:intergenic
AAGTTTCATACACAGGATTT+GGG 0.304860 3_2:+7435098 None:intergenic
TCCCGAACACTAAGGATTAA+TGG 0.308064 3_2:-7434260 Msa0393340:CDS
CTCAAGTTGTCAAGAGAATA+GGG 0.320215 3_2:-7433428 Msa0393340:CDS
TGGAGGCTTTGAAGGAATAT+TGG 0.320768 3_2:+7434031 None:intergenic
TATATTCAACATTTGTTCAT+TGG 0.324970 3_2:-7435208 Msa0393340:five_prime_UTR
TTTAGAAGGAAAAGAAATAA+AGG 0.334192 3_2:-7433604 Msa0393340:CDS
TTTGACTTGGAAAAGTATCT+TGG 0.347158 3_2:+7433898 None:intergenic
TGAAGGAATATTGGTTCCCT+TGG 0.376353 3_2:+7434040 None:intergenic
AACAAGTTGAAGGAACAAAA+AGG 0.378553 3_2:-7433924 Msa0393340:CDS
ACTCTAGTTGGAGGCTTTGA+AGG 0.393913 3_2:+7434023 None:intergenic
TGTTTCAACTTGTGATTTAA+GGG 0.399467 3_2:+7434097 None:intergenic
TTTCTGGAACAGAATATGAT+TGG 0.403613 3_2:+7434195 None:intergenic
TCTCAAGTTGTCAAGAGAAT+AGG 0.415456 3_2:-7433429 Msa0393340:CDS
AACCCTGTCACAATATTACT+TGG 0.426130 3_2:+7433957 None:intergenic
CCAACCCAAGGCAGAATTGA+AGG 0.427450 3_2:-7435074 Msa0393340:CDS
GAAGGAATATTGGTTCCCTT+GGG 0.429823 3_2:+7434041 None:intergenic
TCTCCAAGTAATATTGTGAC+AGG 0.442725 3_2:-7433960 Msa0393340:CDS
CCTTCAATTCTGCCTTGGGT+TGG 0.447147 3_2:+7435074 None:intergenic
GAGGATAAATATCAAATTTG+TGG 0.447215 3_2:-7434288 Msa0393340:CDS
TTTGAATTACATTTCAAGAA+AGG 0.448423 3_2:+7433254 None:intergenic
TTGACTTGGAAAAGTATCTT+GGG 0.454151 3_2:+7433899 None:intergenic
AAGCCTTTAGTTGCAAATAT+GGG 0.459552 3_2:-7433381 Msa0393340:CDS
GGTTCTTCTAGTACTCTAGT+TGG 0.460763 3_2:+7434011 None:intergenic
AGGATAAATATCAAATTTGT+GGG 0.471872 3_2:-7434287 Msa0393340:CDS
GCAAGAAAAGAGTCATCAAA+AGG 0.472949 3_2:-7433582 Msa0393340:CDS
TAGACCTCAAAATGTTTATG+AGG 0.473198 3_2:-7433832 Msa0393340:CDS
GCACCCATATTTGCAACTAA+AGG 0.477234 3_2:+7433378 None:intergenic
TGAGGTCTAGATTCTACAAA+GGG 0.477686 3_2:+7433846 None:intergenic
TCTTCTAGTACTCTAGTTGG+AGG 0.480814 3_2:+7434014 None:intergenic
TCAACTTGTGATTTAAGGGA+TGG 0.486917 3_2:+7434101 None:intergenic
TGAAAAGTATACAAAAGAGA+AGG 0.489401 3_2:-7433481 Msa0393340:CDS
AAAAGATGCAAAGAAGGAAA+AGG 0.493150 3_2:-7433541 Msa0393340:CDS
TGTGAGGTGGAGAAACTTCA+AGG 0.500541 3_2:-7434170 Msa0393340:CDS
AACTACTTGGTAGCAACAAA+TGG 0.500941 3_2:-7434229 Msa0393340:CDS
ATTATATTAATAGCAGAGTC+AGG 0.501722 3_2:+7433152 None:intergenic
ATTCTAGTAATTGTTGCACT+TGG 0.506438 3_2:-7433351 Msa0393340:CDS
GGCTGCAAAAGATGCAAAGA+AGG 0.510641 3_2:-7433547 Msa0393340:CDS
CACTAAGGATTAATGGAGAA+AGG 0.511754 3_2:-7434253 Msa0393340:CDS
ACAATATTACTTGGAGATGA+AGG 0.514944 3_2:+7433966 None:intergenic
TTTCCAAGTCAAAGTCCTAA+GGG 0.518428 3_2:-7433888 Msa0393340:CDS
AAATGACTTGAACAAGTTGA+AGG 0.526624 3_2:-7433934 Msa0393340:CDS
CAACTTGTGATTTAAGGGAT+GGG 0.528671 3_2:+7434102 None:intergenic
ACCTATAATTAGAGACAAGA+AGG 0.529275 3_2:-7433991 Msa0393340:CDS
GGCAGAATTGAAGGAAAATG+AGG 0.534459 3_2:-7435065 Msa0393340:CDS
AATGGAGAAAGGCAACTACT+TGG 0.540238 3_2:-7434242 Msa0393340:CDS
TGGGACTCTCCCGAACACTA+AGG 0.543028 3_2:-7434268 Msa0393340:CDS
CAAAGCAAAGAGGTATAAGA+AGG 0.543303 3_2:-7433505 Msa0393340:CDS
GAAGGGAATCTTCAAAGAAG+TGG 0.546448 3_2:-7433463 Msa0393340:CDS
TTTCCCTTAGGACTTTGACT+TGG 0.546496 3_2:+7433885 None:intergenic
GAAAAGTATACAAAAGAGAA+GGG 0.549687 3_2:-7433480 Msa0393340:CDS
GGTTGGAAAGTTTCATACAC+AGG 0.568545 3_2:+7435091 None:intergenic
TCATACACAGGATTTGGGGA+AGG 0.574199 3_2:+7435103 None:intergenic
TTGAGGTCTAGATTCTACAA+AGG 0.598222 3_2:+7433845 None:intergenic
GCCAAGTCCTAGAACACCCA+AGG 0.612732 3_2:-7434057 Msa0393340:CDS
TGTTCCAGAAAATTGTGAGG+TGG 0.616853 3_2:-7434183 Msa0393340:CDS
GTATGAAACTTTCCAACCCA+AGG 0.630238 3_2:-7435086 Msa0393340:CDS
TCAAGTTGTCAAGAGAATAG+GGG 0.639104 3_2:-7433427 Msa0393340:CDS
CTACAATTGACAAAGCAAAG+AGG 0.640484 3_2:-7433515 Msa0393340:CDS
CTCCAAGTAATATTGTGACA+GGG 0.641452 3_2:-7433959 Msa0393340:CDS
TTCTGTTCCAGAAAATTGTG+AGG 0.646990 3_2:-7434186 Msa0393340:CDS
AGTTTCATACACAGGATTTG+GGG 0.655568 3_2:+7435099 None:intergenic
GTTGTCAAGAGAATAGGGGA+AGG 0.658130 3_2:-7433423 Msa0393340:CDS
CCAAGTCCTAGAACACCCAA+GGG 0.690447 3_2:-7434056 Msa0393340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGCAATAAATAAAATAAA+TGG + chr3_2:7435180-7435199 None:intergenic 10.0%
!! TTAGCAATAAATAAAATAAA+TGG + chr3_2:7435180-7435199 None:intergenic 10.0%
!!! TTATTTTACTTATTAGTCTT+TGG + chr3_2:7433424-7433443 None:intergenic 15.0%
!!! TTTTTTAATTGCTTCTAATA+TGG + chr3_2:7434640-7434659 None:intergenic 15.0%
!!! ATTTTATTTATTGCTAAATG+TGG - chr3_2:7435183-7435202 Msa0393340:five_prime_UTR 15.0%
!!! TTTTATTTATTGCTAAATGT+GGG - chr3_2:7435184-7435203 Msa0393340:five_prime_UTR 15.0%
!!! TTATTTTACTTATTAGTCTT+TGG + chr3_2:7433424-7433443 None:intergenic 15.0%
!!! TTTTTTAATTGCTTCTAATA+TGG + chr3_2:7434640-7434659 None:intergenic 15.0%
!!! ATTTTATTTATTGCTAAATG+TGG - chr3_2:7435183-7435202 Msa0393340:five_prime_UTR 15.0%
!!! TTTTATTTATTGCTAAATGT+GGG - chr3_2:7435184-7435203 Msa0393340:five_prime_UTR 15.0%
!! TATATTCAACATTTGTTCAT+TGG - chr3_2:7433166-7433185 Msa0393340:three_prime_UTR 20.0%
!! AATAAATCAAGAAGCAAAAA+GGG - chr3_2:7433207-7433226 Msa0393340:three_prime_UTR 20.0%
!! CTTTAAATTCTACGTAATAT+TGG - chr3_2:7433392-7433411 Msa0393340:CDS 20.0%
!!! TTTGATTTGTTTTTTGTTCT+AGG - chr3_2:7433457-7433476 Msa0393340:CDS 20.0%
!!! TTAACTAAAGTTGATGTATT+CGG + chr3_2:7433943-7433962 None:intergenic 20.0%
!! AGGATAAATATCAAATTTGT+GGG - chr3_2:7434087-7434106 Msa0393340:CDS 20.0%
!! AAAAAATTCAAGAGGAAAAT+AGG - chr3_2:7434607-7434626 Msa0393340:intron 20.0%
!! TTTAGAAGGAAAAGAAATAA+AGG - chr3_2:7434770-7434789 Msa0393340:intron 20.0%
!! TTTGAATTACATTTCAAGAA+AGG + chr3_2:7435123-7435142 None:intergenic 20.0%
!! ATGAACAAATGTTGAATATA+AGG + chr3_2:7433166-7433185 None:intergenic 20.0%
!! TATATTCAACATTTGTTCAT+TGG - chr3_2:7433166-7433185 Msa0393340:three_prime_UTR 20.0%
!! AATAAATCAAGAAGCAAAAA+GGG - chr3_2:7433207-7433226 Msa0393340:three_prime_UTR 20.0%
!! CTTTAAATTCTACGTAATAT+TGG - chr3_2:7433392-7433411 Msa0393340:CDS 20.0%
!!! TTTGATTTGTTTTTTGTTCT+AGG - chr3_2:7433457-7433476 Msa0393340:CDS 20.0%
!!! TTAACTAAAGTTGATGTATT+CGG + chr3_2:7433943-7433962 None:intergenic 20.0%
!! AGGATAAATATCAAATTTGT+GGG - chr3_2:7434087-7434106 Msa0393340:CDS 20.0%
!! AAAAAATTCAAGAGGAAAAT+AGG - chr3_2:7434607-7434626 Msa0393340:intron 20.0%
!! TTTAGAAGGAAAAGAAATAA+AGG - chr3_2:7434770-7434789 Msa0393340:intron 20.0%
!! TTTGAATTACATTTCAAGAA+AGG + chr3_2:7435123-7435142 None:intergenic 20.0%
! GAATAAATCAAGAAGCAAAA+AGG - chr3_2:7433206-7433225 Msa0393340:three_prime_UTR 25.0%
! TAAATTCTACGTAATATTGG+AGG - chr3_2:7433395-7433414 Msa0393340:CDS 25.0%
! AAATTCTACGTAATATTGGA+GGG - chr3_2:7433396-7433415 Msa0393340:CDS 25.0%
! AAATGTGATCCTTTCTAATA+TGG + chr3_2:7433495-7433514 None:intergenic 25.0%
!! CTTTTACAAAAAGGATCAAA+GGG + chr3_2:7433875-7433894 None:intergenic 25.0%
!! TCTTTTACAAAAAGGATCAA+AGG + chr3_2:7433876-7433895 None:intergenic 25.0%
! GAGGATAAATATCAAATTTG+TGG - chr3_2:7434086-7434105 Msa0393340:CDS 25.0%
! TGTTTCAACTTGTGATTTAA+GGG + chr3_2:7434280-7434299 None:intergenic 25.0%
! TTGTTTCAACTTGTGATTTA+AGG + chr3_2:7434281-7434300 None:intergenic 25.0%
!! TTTTCCTCATAAACATTTTG+AGG + chr3_2:7434549-7434568 None:intergenic 25.0%
!! TTTTTCATCATCTATTGCTT+GGG + chr3_2:7434592-7434611 None:intergenic 25.0%
!!! TTTTTTCATCATCTATTGCT+TGG + chr3_2:7434593-7434612 None:intergenic 25.0%
! AGATGATGAAAAAATTCAAG+AGG - chr3_2:7434599-7434618 Msa0393340:intron 25.0%
! AATTAAAAAACAATTGCACG+AGG - chr3_2:7434650-7434669 Msa0393340:intron 25.0%
! ATCAAAAGGAGAAGAATATT+TGG - chr3_2:7434806-7434825 Msa0393340:intron 25.0%
!! TGAAAAGTATACAAAAGAGA+AGG - chr3_2:7434893-7434912 Msa0393340:intron 25.0%
!! GAAAAGTATACAAAAGAGAA+GGG - chr3_2:7434894-7434913 Msa0393340:intron 25.0%
! GAATAAATCAAGAAGCAAAA+AGG - chr3_2:7433206-7433225 Msa0393340:three_prime_UTR 25.0%
! TAAATTCTACGTAATATTGG+AGG - chr3_2:7433395-7433414 Msa0393340:CDS 25.0%
! AAATTCTACGTAATATTGGA+GGG - chr3_2:7433396-7433415 Msa0393340:CDS 25.0%
! AAATGTGATCCTTTCTAATA+TGG + chr3_2:7433495-7433514 None:intergenic 25.0%
!! CTTTTACAAAAAGGATCAAA+GGG + chr3_2:7433875-7433894 None:intergenic 25.0%
!! TCTTTTACAAAAAGGATCAA+AGG + chr3_2:7433876-7433895 None:intergenic 25.0%
! GAGGATAAATATCAAATTTG+TGG - chr3_2:7434086-7434105 Msa0393340:CDS 25.0%
! TGTTTCAACTTGTGATTTAA+GGG + chr3_2:7434280-7434299 None:intergenic 25.0%
! TTGTTTCAACTTGTGATTTA+AGG + chr3_2:7434281-7434300 None:intergenic 25.0%
!! TTTTCCTCATAAACATTTTG+AGG + chr3_2:7434549-7434568 None:intergenic 25.0%
!! TTTTTCATCATCTATTGCTT+GGG + chr3_2:7434592-7434611 None:intergenic 25.0%
!!! TTTTTTCATCATCTATTGCT+TGG + chr3_2:7434593-7434612 None:intergenic 25.0%
! AGATGATGAAAAAATTCAAG+AGG - chr3_2:7434599-7434618 Msa0393340:intron 25.0%
! AATTAAAAAACAATTGCACG+AGG - chr3_2:7434650-7434669 Msa0393340:intron 25.0%
! ATCAAAAGGAGAAGAATATT+TGG - chr3_2:7434806-7434825 Msa0393340:intron 25.0%
!! TGAAAAGTATACAAAAGAGA+AGG - chr3_2:7434893-7434912 Msa0393340:intron 25.0%
!! GAAAAGTATACAAAAGAGAA+GGG - chr3_2:7434894-7434913 Msa0393340:intron 25.0%
AAGTTTCATACACAGGATTT+GGG + chr3_2:7433279-7433298 None:intergenic 30.0%
AAAGTTTCATACACAGGATT+TGG + chr3_2:7433280-7433299 None:intergenic 30.0%
AAAAACAAAAAATACGCACC+AGG + chr3_2:7433361-7433380 None:intergenic 30.0%
AATTCTACGTAATATTGGAG+GGG - chr3_2:7433397-7433416 Msa0393340:CDS 30.0%
!! TTGATTTGGTTGTATTGACT+TGG - chr3_2:7433624-7433643 Msa0393340:CDS 30.0%
!! TGATTTGGTTGTATTGACTT+GGG - chr3_2:7433625-7433644 Msa0393340:CDS 30.0%
! CCTACTTGTCTTTTACAAAA+AGG + chr3_2:7433884-7433903 None:intergenic 30.0%
! CCTTTTTGTAAAAGACAAGT+AGG - chr3_2:7433881-7433900 Msa0393340:CDS 30.0%
!! TGTTCTGCAAGGTTTTATAA+AGG - chr3_2:7434062-7434081 Msa0393340:CDS 30.0%
! CAGAATATGATTGGTCAAAT+AGG + chr3_2:7434173-7434192 None:intergenic 30.0%
!! TTTCTGGAACAGAATATGAT+TGG + chr3_2:7434182-7434201 None:intergenic 30.0%
!!! ACTTGGCACAATTTTTTCTT+TGG + chr3_2:7434304-7434323 None:intergenic 30.0%
ACCTATAATTAGAGACAAGA+AGG - chr3_2:7434383-7434402 Msa0393340:intron 30.0%
ACCTTCTTGTCTCTAATTAT+AGG + chr3_2:7434387-7434406 None:intergenic 30.0%
ACAATATTACTTGGAGATGA+AGG + chr3_2:7434411-7434430 None:intergenic 30.0%
! AAATGACTTGAACAAGTTGA+AGG - chr3_2:7434440-7434459 Msa0393340:intron 30.0%
! AACAAGTTGAAGGAACAAAA+AGG - chr3_2:7434450-7434469 Msa0393340:intron 30.0%
!! TTGACTTGGAAAAGTATCTT+GGG + chr3_2:7434478-7434497 None:intergenic 30.0%
!! TTTGACTTGGAAAAGTATCT+TGG + chr3_2:7434479-7434498 None:intergenic 30.0%
! AAAGCTTTCAATTTTCCCTT+AGG + chr3_2:7434504-7434523 None:intergenic 30.0%
TAGACCTCAAAATGTTTATG+AGG - chr3_2:7434542-7434561 Msa0393340:intron 30.0%
!! GATCACAATACGTTTTTAGA+AGG - chr3_2:7434756-7434775 Msa0393340:intron 30.0%
AAAAGATGCAAAGAAGGAAA+AGG - chr3_2:7434833-7434852 Msa0393340:intron 30.0%
AAAGCCTTTAGTTGCAAATA+TGG - chr3_2:7434992-7435011 Msa0393340:intron 30.0%
AAGCCTTTAGTTGCAAATAT+GGG - chr3_2:7434993-7435012 Msa0393340:intron 30.0%
ATTCTAGTAATTGTTGCACT+TGG - chr3_2:7435023-7435042 Msa0393340:intron 30.0%
GCTAGAAGAGATAAACTTAT+AGG + chr3_2:7435060-7435079 None:intergenic 30.0%
AAGTTTCATACACAGGATTT+GGG + chr3_2:7433279-7433298 None:intergenic 30.0%
AAAGTTTCATACACAGGATT+TGG + chr3_2:7433280-7433299 None:intergenic 30.0%
AAAAACAAAAAATACGCACC+AGG + chr3_2:7433361-7433380 None:intergenic 30.0%
AATTCTACGTAATATTGGAG+GGG - chr3_2:7433397-7433416 Msa0393340:CDS 30.0%
!! TTGATTTGGTTGTATTGACT+TGG - chr3_2:7433624-7433643 Msa0393340:CDS 30.0%
!! TGATTTGGTTGTATTGACTT+GGG - chr3_2:7433625-7433644 Msa0393340:CDS 30.0%
! CCTACTTGTCTTTTACAAAA+AGG + chr3_2:7433884-7433903 None:intergenic 30.0%
! CCTTTTTGTAAAAGACAAGT+AGG - chr3_2:7433881-7433900 Msa0393340:CDS 30.0%
!! TGTTCTGCAAGGTTTTATAA+AGG - chr3_2:7434062-7434081 Msa0393340:CDS 30.0%
! CAGAATATGATTGGTCAAAT+AGG + chr3_2:7434173-7434192 None:intergenic 30.0%
!! TTTCTGGAACAGAATATGAT+TGG + chr3_2:7434182-7434201 None:intergenic 30.0%
!!! ACTTGGCACAATTTTTTCTT+TGG + chr3_2:7434304-7434323 None:intergenic 30.0%
ACCTATAATTAGAGACAAGA+AGG - chr3_2:7434383-7434402 Msa0393340:intron 30.0%
ACCTTCTTGTCTCTAATTAT+AGG + chr3_2:7434387-7434406 None:intergenic 30.0%
ACAATATTACTTGGAGATGA+AGG + chr3_2:7434411-7434430 None:intergenic 30.0%
! AAATGACTTGAACAAGTTGA+AGG - chr3_2:7434440-7434459 Msa0393340:intron 30.0%
! AACAAGTTGAAGGAACAAAA+AGG - chr3_2:7434450-7434469 Msa0393340:intron 30.0%
!! TTGACTTGGAAAAGTATCTT+GGG + chr3_2:7434478-7434497 None:intergenic 30.0%
!! TTTGACTTGGAAAAGTATCT+TGG + chr3_2:7434479-7434498 None:intergenic 30.0%
! AAAGCTTTCAATTTTCCCTT+AGG + chr3_2:7434504-7434523 None:intergenic 30.0%
TAGACCTCAAAATGTTTATG+AGG - chr3_2:7434542-7434561 Msa0393340:intron 30.0%
!! GATCACAATACGTTTTTAGA+AGG - chr3_2:7434756-7434775 Msa0393340:intron 30.0%
AAAAGATGCAAAGAAGGAAA+AGG - chr3_2:7434833-7434852 Msa0393340:intron 30.0%
AAAGCCTTTAGTTGCAAATA+TGG - chr3_2:7434992-7435011 Msa0393340:intron 30.0%
AAGCCTTTAGTTGCAAATAT+GGG - chr3_2:7434993-7435012 Msa0393340:intron 30.0%
ATTCTAGTAATTGTTGCACT+TGG - chr3_2:7435023-7435042 Msa0393340:intron 30.0%
GCTAGAAGAGATAAACTTAT+AGG + chr3_2:7435060-7435079 None:intergenic 30.0%
AGTTTCATACACAGGATTTG+GGG + chr3_2:7433278-7433297 None:intergenic 35.0%
! TTTTCCTTCAATTCTGCCTT+GGG + chr3_2:7433307-7433326 None:intergenic 35.0%
! ATTTTCCTTCAATTCTGCCT+TGG + chr3_2:7433308-7433327 None:intergenic 35.0%
TGTGATCCTTTCTAATATGG+AGG + chr3_2:7433492-7433511 None:intergenic 35.0%
TTGTCTTAAGAAAACCCACT+TGG - chr3_2:7433600-7433619 Msa0393340:CDS 35.0%
TGTCTTAAGAAAACCCACTT+GGG - chr3_2:7433601-7433620 Msa0393340:CDS 35.0%
ATTCTCCTCGATGTCAATTT+GGG - chr3_2:7433687-7433706 Msa0393340:CDS 35.0%
!! TGCAAGGTTTTATAAAGGAG+AGG - chr3_2:7434067-7434086 Msa0393340:CDS 35.0%
TCCATTAATCCTTAGTGTTC+GGG + chr3_2:7434118-7434137 None:intergenic 35.0%
CTCCATTAATCCTTAGTGTT+CGG + chr3_2:7434119-7434138 None:intergenic 35.0%
CACTAAGGATTAATGGAGAA+AGG - chr3_2:7434121-7434140 Msa0393340:CDS 35.0%
! AACTACTTGGTAGCAACAAA+TGG - chr3_2:7434145-7434164 Msa0393340:CDS 35.0%
TTCTGTTCCAGAAAATTGTG+AGG - chr3_2:7434188-7434207 Msa0393340:CDS 35.0%
CTTCAAGGCAAGTTTGAAAA+TGG - chr3_2:7434219-7434238 Msa0393340:CDS 35.0%
!!! TTAAGGGATGGGAACTTTTT+TGG + chr3_2:7434264-7434283 None:intergenic 35.0%
CAACTTGTGATTTAAGGGAT+GGG + chr3_2:7434275-7434294 None:intergenic 35.0%
TCAACTTGTGATTTAAGGGA+TGG + chr3_2:7434276-7434295 None:intergenic 35.0%
TCTCCAAGTAATATTGTGAC+AGG - chr3_2:7434414-7434433 Msa0393340:intron 35.0%
CTCCAAGTAATATTGTGACA+GGG - chr3_2:7434415-7434434 Msa0393340:intron 35.0%
AACCCTGTCACAATATTACT+TGG + chr3_2:7434420-7434439 None:intergenic 35.0%
! TTTTCCAAGTCAAAGTCCTA+AGG - chr3_2:7434485-7434504 Msa0393340:intron 35.0%
TTTCCAAGTCAAAGTCCTAA+GGG - chr3_2:7434486-7434505 Msa0393340:intron 35.0%
TGAGGTCTAGATTCTACAAA+GGG + chr3_2:7434531-7434550 None:intergenic 35.0%
TTGAGGTCTAGATTCTACAA+AGG + chr3_2:7434532-7434551 None:intergenic 35.0%
GCAAGAAAAGAGTCATCAAA+AGG - chr3_2:7434792-7434811 Msa0393340:intron 35.0%
CTACAATTGACAAAGCAAAG+AGG - chr3_2:7434859-7434878 Msa0393340:intron 35.0%
! CAAAGCAAAGAGGTATAAGA+AGG - chr3_2:7434869-7434888 Msa0393340:intron 35.0%
! TCTCAAGTTGTCAAGAGAAT+AGG - chr3_2:7434945-7434964 Msa0393340:intron 35.0%
! CTCAAGTTGTCAAGAGAATA+GGG - chr3_2:7434946-7434965 Msa0393340:intron 35.0%
! TCAAGTTGTCAAGAGAATAG+GGG - chr3_2:7434947-7434966 Msa0393340:intron 35.0%
AGTTTCATACACAGGATTTG+GGG + chr3_2:7433278-7433297 None:intergenic 35.0%
! TTTTCCTTCAATTCTGCCTT+GGG + chr3_2:7433307-7433326 None:intergenic 35.0%
! ATTTTCCTTCAATTCTGCCT+TGG + chr3_2:7433308-7433327 None:intergenic 35.0%
TGTGATCCTTTCTAATATGG+AGG + chr3_2:7433492-7433511 None:intergenic 35.0%
TTGTCTTAAGAAAACCCACT+TGG - chr3_2:7433600-7433619 Msa0393340:CDS 35.0%
TGTCTTAAGAAAACCCACTT+GGG - chr3_2:7433601-7433620 Msa0393340:CDS 35.0%
ATTCTCCTCGATGTCAATTT+GGG - chr3_2:7433687-7433706 Msa0393340:CDS 35.0%
!! TGCAAGGTTTTATAAAGGAG+AGG - chr3_2:7434067-7434086 Msa0393340:CDS 35.0%
TCCATTAATCCTTAGTGTTC+GGG + chr3_2:7434118-7434137 None:intergenic 35.0%
CTCCATTAATCCTTAGTGTT+CGG + chr3_2:7434119-7434138 None:intergenic 35.0%
CACTAAGGATTAATGGAGAA+AGG - chr3_2:7434121-7434140 Msa0393340:CDS 35.0%
! AACTACTTGGTAGCAACAAA+TGG - chr3_2:7434145-7434164 Msa0393340:CDS 35.0%
TTCTGTTCCAGAAAATTGTG+AGG - chr3_2:7434188-7434207 Msa0393340:CDS 35.0%
CTTCAAGGCAAGTTTGAAAA+TGG - chr3_2:7434219-7434238 Msa0393340:CDS 35.0%
!!! TTAAGGGATGGGAACTTTTT+TGG + chr3_2:7434264-7434283 None:intergenic 35.0%
CAACTTGTGATTTAAGGGAT+GGG + chr3_2:7434275-7434294 None:intergenic 35.0%
TCAACTTGTGATTTAAGGGA+TGG + chr3_2:7434276-7434295 None:intergenic 35.0%
TCTCCAAGTAATATTGTGAC+AGG - chr3_2:7434414-7434433 Msa0393340:intron 35.0%
CTCCAAGTAATATTGTGACA+GGG - chr3_2:7434415-7434434 Msa0393340:intron 35.0%
AACCCTGTCACAATATTACT+TGG + chr3_2:7434420-7434439 None:intergenic 35.0%
! TTTTCCAAGTCAAAGTCCTA+AGG - chr3_2:7434485-7434504 Msa0393340:intron 35.0%
TTTCCAAGTCAAAGTCCTAA+GGG - chr3_2:7434486-7434505 Msa0393340:intron 35.0%
TGAGGTCTAGATTCTACAAA+GGG + chr3_2:7434531-7434550 None:intergenic 35.0%
TTGAGGTCTAGATTCTACAA+AGG + chr3_2:7434532-7434551 None:intergenic 35.0%
GCAAGAAAAGAGTCATCAAA+AGG - chr3_2:7434792-7434811 Msa0393340:intron 35.0%
CTACAATTGACAAAGCAAAG+AGG - chr3_2:7434859-7434878 Msa0393340:intron 35.0%
! CAAAGCAAAGAGGTATAAGA+AGG - chr3_2:7434869-7434888 Msa0393340:intron 35.0%
! TCTCAAGTTGTCAAGAGAAT+AGG - chr3_2:7434945-7434964 Msa0393340:intron 35.0%
! CTCAAGTTGTCAAGAGAATA+GGG - chr3_2:7434946-7434965 Msa0393340:intron 35.0%
! TCAAGTTGTCAAGAGAATAG+GGG - chr3_2:7434947-7434966 Msa0393340:intron 35.0%
TCAAGAAGCAAAAAGGGCTA+TGG - chr3_2:7433213-7433232 Msa0393340:three_prime_UTR 40.0%
!!! GAAGGTTTTGTTCTAGAGGT+AGG + chr3_2:7433256-7433275 None:intergenic 40.0%
GGTTGGAAAGTTTCATACAC+AGG + chr3_2:7433286-7433305 None:intergenic 40.0%
GTATGAAACTTTCCAACCCA+AGG - chr3_2:7433288-7433307 Msa0393340:three_prime_UTR 40.0%
GGCAGAATTGAAGGAAAATG+AGG - chr3_2:7433309-7433328 Msa0393340:CDS 40.0%
TTTCTTCACATTCACCTTCC+TGG - chr3_2:7433340-7433359 Msa0393340:CDS 40.0%
ACAAAAAATACGCACCAGGA+AGG + chr3_2:7433357-7433376 None:intergenic 40.0%
TGCACTCCTCCATATTAGAA+AGG - chr3_2:7433483-7433502 Msa0393340:CDS 40.0%
!! AAAACCCACTTGGGTTGATT+TGG - chr3_2:7433610-7433629 Msa0393340:CDS 40.0%
ACAACCAAATCAACCCAAGT+GGG + chr3_2:7433617-7433636 None:intergenic 40.0%
TACAACCAAATCAACCCAAG+TGG + chr3_2:7433618-7433637 None:intergenic 40.0%
! TGAGAGTGTACTCATTCTCA+AGG - chr3_2:7433653-7433672 Msa0393340:CDS 40.0%
GATTCTCCTCGATGTCAATT+TGG - chr3_2:7433686-7433705 Msa0393340:CDS 40.0%
TATCACCCAAATTGACATCG+AGG + chr3_2:7433695-7433714 None:intergenic 40.0%
TCCCGAACACTAAGGATTAA+TGG - chr3_2:7434114-7434133 Msa0393340:CDS 40.0%
AATGGAGAAAGGCAACTACT+TGG - chr3_2:7434132-7434151 Msa0393340:CDS 40.0%
TGTTCCAGAAAATTGTGAGG+TGG - chr3_2:7434191-7434210 Msa0393340:CDS 40.0%
! TTCTCCACCTCACAATTTTC+TGG + chr3_2:7434198-7434217 None:intergenic 40.0%
!! GAAGGAATATTGGTTCCCTT+GGG + chr3_2:7434336-7434355 None:intergenic 40.0%
!! TGAAGGAATATTGGTTCCCT+TGG + chr3_2:7434337-7434356 None:intergenic 40.0%
!! TGGAGGCTTTGAAGGAATAT+TGG + chr3_2:7434346-7434365 None:intergenic 40.0%
TCTTCTAGTACTCTAGTTGG+AGG + chr3_2:7434363-7434382 None:intergenic 40.0%
GGTTCTTCTAGTACTCTAGT+TGG + chr3_2:7434366-7434385 None:intergenic 40.0%
TTTCCCTTAGGACTTTGACT+TGG + chr3_2:7434492-7434511 None:intergenic 40.0%
GAAGGGAATCTTCAAAGAAG+TGG - chr3_2:7434911-7434930 Msa0393340:intron 40.0%
GCACCCATATTTGCAACTAA+AGG + chr3_2:7434999-7435018 None:intergenic 40.0%
TCAAGAAGCAAAAAGGGCTA+TGG - chr3_2:7433213-7433232 Msa0393340:three_prime_UTR 40.0%
!!! GAAGGTTTTGTTCTAGAGGT+AGG + chr3_2:7433256-7433275 None:intergenic 40.0%
GGTTGGAAAGTTTCATACAC+AGG + chr3_2:7433286-7433305 None:intergenic 40.0%
GTATGAAACTTTCCAACCCA+AGG - chr3_2:7433288-7433307 Msa0393340:three_prime_UTR 40.0%
GGCAGAATTGAAGGAAAATG+AGG - chr3_2:7433309-7433328 Msa0393340:CDS 40.0%
TTTCTTCACATTCACCTTCC+TGG - chr3_2:7433340-7433359 Msa0393340:CDS 40.0%
ACAAAAAATACGCACCAGGA+AGG + chr3_2:7433357-7433376 None:intergenic 40.0%
TGCACTCCTCCATATTAGAA+AGG - chr3_2:7433483-7433502 Msa0393340:CDS 40.0%
!! AAAACCCACTTGGGTTGATT+TGG - chr3_2:7433610-7433629 Msa0393340:CDS 40.0%
ACAACCAAATCAACCCAAGT+GGG + chr3_2:7433617-7433636 None:intergenic 40.0%
TACAACCAAATCAACCCAAG+TGG + chr3_2:7433618-7433637 None:intergenic 40.0%
! TGAGAGTGTACTCATTCTCA+AGG - chr3_2:7433653-7433672 Msa0393340:CDS 40.0%
GATTCTCCTCGATGTCAATT+TGG - chr3_2:7433686-7433705 Msa0393340:CDS 40.0%
TATCACCCAAATTGACATCG+AGG + chr3_2:7433695-7433714 None:intergenic 40.0%
TCCCGAACACTAAGGATTAA+TGG - chr3_2:7434114-7434133 Msa0393340:CDS 40.0%
AATGGAGAAAGGCAACTACT+TGG - chr3_2:7434132-7434151 Msa0393340:CDS 40.0%
TGTTCCAGAAAATTGTGAGG+TGG - chr3_2:7434191-7434210 Msa0393340:CDS 40.0%
! TTCTCCACCTCACAATTTTC+TGG + chr3_2:7434198-7434217 None:intergenic 40.0%
!! GAAGGAATATTGGTTCCCTT+GGG + chr3_2:7434336-7434355 None:intergenic 40.0%
!! TGAAGGAATATTGGTTCCCT+TGG + chr3_2:7434337-7434356 None:intergenic 40.0%
!! TGGAGGCTTTGAAGGAATAT+TGG + chr3_2:7434346-7434365 None:intergenic 40.0%
TCTTCTAGTACTCTAGTTGG+AGG + chr3_2:7434363-7434382 None:intergenic 40.0%
GGTTCTTCTAGTACTCTAGT+TGG + chr3_2:7434366-7434385 None:intergenic 40.0%
TTTCCCTTAGGACTTTGACT+TGG + chr3_2:7434492-7434511 None:intergenic 40.0%
GAAGGGAATCTTCAAAGAAG+TGG - chr3_2:7434911-7434930 Msa0393340:intron 40.0%
GCACCCATATTTGCAACTAA+AGG + chr3_2:7434999-7435018 None:intergenic 40.0%
!!! TGGGGAAGGTTTTGTTCTAG+AGG + chr3_2:7433260-7433279 None:intergenic 45.0%
TCATACACAGGATTTGGGGA+AGG + chr3_2:7433274-7433293 None:intergenic 45.0%
GAGAATGAGTACACTCTCAC+AGG + chr3_2:7433652-7433671 None:intergenic 45.0%
CTTCTCTCTCATGTTCTGCA+AGG - chr3_2:7434051-7434070 Msa0393340:CDS 45.0%
TGTGAGGTGGAGAAACTTCA+AGG - chr3_2:7434204-7434223 Msa0393340:CDS 45.0%
! ACTCTAGTTGGAGGCTTTGA+AGG + chr3_2:7434354-7434373 None:intergenic 45.0%
GGCTGCAAAAGATGCAAAGA+AGG - chr3_2:7434827-7434846 Msa0393340:intron 45.0%
GTTGTCAAGAGAATAGGGGA+AGG - chr3_2:7434951-7434970 Msa0393340:intron 45.0%
!!! TGGGGAAGGTTTTGTTCTAG+AGG + chr3_2:7433260-7433279 None:intergenic 45.0%
TCATACACAGGATTTGGGGA+AGG + chr3_2:7433274-7433293 None:intergenic 45.0%
GAGAATGAGTACACTCTCAC+AGG + chr3_2:7433652-7433671 None:intergenic 45.0%
CTTCTCTCTCATGTTCTGCA+AGG - chr3_2:7434051-7434070 Msa0393340:CDS 45.0%
TGTGAGGTGGAGAAACTTCA+AGG - chr3_2:7434204-7434223 Msa0393340:CDS 45.0%
! ACTCTAGTTGGAGGCTTTGA+AGG + chr3_2:7434354-7434373 None:intergenic 45.0%
GGCTGCAAAAGATGCAAAGA+AGG - chr3_2:7434827-7434846 Msa0393340:intron 45.0%
GTTGTCAAGAGAATAGGGGA+AGG - chr3_2:7434951-7434970 Msa0393340:intron 45.0%
CCTTCAATTCTGCCTTGGGT+TGG + chr3_2:7433303-7433322 None:intergenic 50.0%
CCAACCCAAGGCAGAATTGA+AGG - chr3_2:7433300-7433319 Msa0393340:exon 50.0%
CCAAGTCCTAGAACACCCAA+GGG - chr3_2:7434318-7434337 Msa0393340:intron 50.0%
TTGGTTCCCTTGGGTGTTCT+AGG + chr3_2:7434327-7434346 None:intergenic 50.0%
CCTTCAATTCTGCCTTGGGT+TGG + chr3_2:7433303-7433322 None:intergenic 50.0%
CCAACCCAAGGCAGAATTGA+AGG - chr3_2:7433300-7433319 Msa0393340:exon 50.0%
CCAAGTCCTAGAACACCCAA+GGG - chr3_2:7434318-7434337 Msa0393340:intron 50.0%
TTGGTTCCCTTGGGTGTTCT+AGG + chr3_2:7434327-7434346 None:intergenic 50.0%
TGGGACTCTCCCGAACACTA+AGG - chr3_2:7434106-7434125 Msa0393340:CDS 55.0%
GCCAAGTCCTAGAACACCCA+AGG - chr3_2:7434317-7434336 Msa0393340:intron 55.0%
!! CCCTTGGGTGTTCTAGGACT+TGG + chr3_2:7434321-7434340 None:intergenic 55.0%
TGGGACTCTCCCGAACACTA+AGG - chr3_2:7434106-7434125 Msa0393340:CDS 55.0%
GCCAAGTCCTAGAACACCCA+AGG - chr3_2:7434317-7434336 Msa0393340:intron 55.0%
!! CCCTTGGGTGTTCTAGGACT+TGG + chr3_2:7434321-7434340 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 7433164 7435232 7433164 ID=Msa0393340;Name=Msa0393340
chr3_2 mRNA 7433164 7435232 7433164 ID=Msa0393340-mRNA-1;Parent=Msa0393340;Name=Msa0393340-mRNA-1;_AED=0.01;_eAED=0.01;_QI=68|1|1|1|1|1|2|144|381
chr3_2 exon 7433164 7434323 7433164 ID=Msa0393340-mRNA-1:exon:1855;Parent=Msa0393340-mRNA-1
chr3_2 exon 7435035 7435232 7435035 ID=Msa0393340-mRNA-1:exon:1854;Parent=Msa0393340-mRNA-1
chr3_2 five_prime_UTR 7435165 7435232 7435165 ID=Msa0393340-mRNA-1:five_prime_utr;Parent=Msa0393340-mRNA-1
chr3_2 CDS 7435035 7435164 7435035 ID=Msa0393340-mRNA-1:cds;Parent=Msa0393340-mRNA-1
chr3_2 CDS 7433308 7434323 7433308 ID=Msa0393340-mRNA-1:cds;Parent=Msa0393340-mRNA-1
chr3_2 three_prime_UTR 7433164 7433307 7433164 ID=Msa0393340-mRNA-1:three_prime_utr;Parent=Msa0393340-mRNA-1
Gene Sequence

>Msa0393340

ATGGCTATGAAATCAAGAACACCTACCTCTAGAACAAAACCTTCCCCAAATCCTGTGTATGAAACTTTCCAACCCAAGGCAGAATTGAAGGAAAATGAGGAAGCATATTTTCTTCACATTCACCTTCCTGGTTTTATAAAGGAGAGGATAAATATCAAATTTGTGGGACTCTCCCGAACACTAAGGATTAATGGAGAAAGGCAACTACTTGGTAGCAACAAATGGAGCCTATTTGACCAATCATATTCTGTTCCAGAAAATTGTGAGGTGGAGAAACTTCAAGGCAAGTTTGAAAATGGAACTCTCATTGTTGCAATGCCAAAAAAGTTCCCATCCCTTAAATCACAAGTTGAAACAACCAAAGAAAAAATTGTGCCAAGTCCTAGAACACCCAAGGGAACCAATATTCCTTCAAAGCCTCCAACTAGAGTACTAGAAGAACCTATAATTAGAGACAAGAAGGTTCCTTCATCTCCAAGTAATATTGTGACAGGGTTAAATGACTTGAACAAGTTGAAGGAACAAAAAGGTACCCAAGATACTTTTCCAAGTCAAAGTCCTAAGGGAAAATTGAAAGCTTTAGAACCCTTTGTAGAATCTAGACCTCAAAATGTTTATGAGGAAAATAATGTGCTAAAAGAAAAATCCCAAGCAATAGATGATGAAAAAATTCAAGAGGAAAATAGGAAAAAGTCCATATTAGAAGCAATTAAAAAACAATTGCACGAGGACGAAAAGAAAAGCGTCACAAATAAGAAAATTGAAGAAGATAAGAAGACTTACGAGTCAAGCAAGCTTGATCAACAATACGTTGATCACAATACGTTTTTAGAAGGAAAAGAAATAAAGGCAAGAAAAGAGTCATCAAAAGGAGAAGAATATTTGGCTGCAAAAGATGCAAAGAAGGAAAAGGATACTACAATTGACAAAGCAAAGAGGTATAAGAAGGATGAAAAGTATACAAAAGAGAAGGGAATCTTCAAAGAAGTGGCAACTTCAGCTTCTCAAGTTGTCAAGAGAATAGGGGAAGGAAAATTGAATGAACAAGAAAAGCCTTTAGTTGCAAATATGGGTGCTGCAATTCTAGTAATTGTTGCACTTGGAGCTTATGCTACCTATAAGTTTATCTCTTCTAGCAAAAATTAA
Protein sequence

>Msa0393340

MAMKSRTPTSRTKPSPNPVYETFQPKAELKENEEAYFLHIHLPGFIKERINIKFVGLSRTLRINGERQLLGSNKWSLFDQSYSVPENCEVEKLQGKFENGTLIVAMPKKFPSLKSQVETTKEKIVPSPRTPKGTNIPSKPPTRVLEEPIIRDKKVPSSPSNIVTGLNDLNKLKEQKGTQDTFPSQSPKGKLKALEPFVESRPQNVYEENNVLKEKSQAIDDEKIQEENRKKSILEAIKKQLHEDEKKSVTNKKIEEDKKTYESSKLDQQYVDHNTFLEGKEIKARKESSKGEEYLAAKDAKKEKDTTIDKAKRYKKDEKYTKEKGIFKEVATSASQVVKRIGEGKLNEQEKPLVANMGAAILVIVALGAYATYKFISSSKN*