AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0457770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0457770 MtrunA17_Chr3g0087291 94.939 573 19 2 1 573 1 563 0.0 1116
Msa0457770 MtrunA17_Chr4g0010511 69.342 623 141 8 1 573 1 623 0.0 795
Msa0457770 MtrunA17_Chr4g0070551 36.490 433 259 7 143 566 192 617 3.46e-86 280
Msa0457770 MtrunA17_Chr5g0426591 38.326 454 229 13 143 566 79 511 2.71e-84 272
Msa0457770 MtrunA17_Chr3g0129981 36.735 441 246 11 147 566 83 511 6.09e-82 266
Msa0457770 MtrunA17_Chr4g0008451 35.963 431 254 9 152 570 205 625 8.15e-80 263
Msa0457770 MtrunA17_Chr3g0132371 35.345 464 245 10 142 573 105 545 3.33e-78 256
Msa0457770 MtrunA17_Chr5g0448581 36.300 427 253 9 150 569 88 502 1.76e-77 256
Msa0457770 MtrunA17_Chr3g0143821 36.761 457 258 15 128 571 59 497 1.63e-76 253
Msa0457770 MtrunA17_Chr6g0455571 38.503 374 216 6 186 549 118 487 1.25e-75 249
Msa0457770 MtrunA17_Chr4g0056061 38.243 387 225 8 186 566 111 489 1.82e-75 249
Msa0457770 MtrunA17_Chr5g0399311 36.156 437 263 9 136 566 79 505 8.44e-75 247
Msa0457770 MtrunA17_Chr5g0436321 37.179 390 233 6 186 566 164 550 1.49e-74 248
Msa0457770 MtrunA17_Chr4g0042711 36.683 398 233 9 186 569 100 492 6.09e-73 241
Msa0457770 MtrunA17_Chr4g0061781 37.337 383 223 8 177 548 111 487 9.08e-71 236
Msa0457770 MtrunA17_Chr5g0412251 34.279 458 244 9 151 566 59 501 1.07e-70 236
Msa0457770 MtrunA17_Chr4g0043911 33.710 442 263 10 144 566 117 547 8.13e-70 235
Msa0457770 MtrunA17_Chr2g0307971 36.957 368 218 6 186 543 118 481 9.21e-70 233
Msa0457770 MtrunA17_Chr5g0417871 35.075 402 242 8 186 573 88 484 3.83e-68 228
Msa0457770 MtrunA17_Chr1g0160781 33.260 454 274 10 133 567 104 547 7.02e-68 229
Msa0457770 MtrunA17_Chr3g0132951 32.548 467 246 9 148 566 70 515 1.80e-66 225
Msa0457770 MtrunA17_Chr5g0424241 32.731 443 276 7 144 566 119 559 4.14e-66 226
Msa0457770 MtrunA17_Chr1g0204121 30.841 428 275 9 152 569 61 477 6.85e-66 223
Msa0457770 MtrunA17_Chr3g0123521 33.032 442 265 12 148 566 114 547 6.17e-65 221
Msa0457770 MtrunA17_Chr3g0077321 32.558 430 265 9 154 566 123 544 3.25e-63 217
Msa0457770 MtrunA17_Chr1g0204141 29.167 432 280 11 152 569 61 480 2.23e-55 195
Msa0457770 MtrunA17_Chr2g0284071 28.970 466 278 14 148 566 181 640 1.10e-46 173
Msa0457770 MtrunA17_Chr4g0075311 30.626 431 243 12 186 566 167 591 3.92e-46 171
Msa0457770 MtrunA17_Chr4g0003561 26.786 392 245 12 186 566 78 438 4.28e-33 131
Msa0457770 MtrunA17_Chr7g0220381 29.655 290 189 6 186 460 101 390 1.20e-30 127
Msa0457770 MtrunA17_Chr1g0146501 30.272 294 190 6 186 464 93 386 4.57e-29 122
Msa0457770 MtrunA17_Chr3g0127811 28.660 321 206 8 158 464 35 346 1.55e-27 115
Msa0457770 MtrunA17_Chr4g0002361 25.581 344 195 13 186 511 89 389 1.54e-20 94.4
Msa0457770 MtrunA17_Chr2g0298881 25.127 394 225 16 151 519 5 353 1.04e-19 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0457770 AT2G44500.1 67.372 567 156 6 11 568 17 563 0.0 753
Msa0457770 AT3G07900.1 62.153 576 192 9 10 571 14 577 0.0 692
Msa0457770 AT5G63390.1 61.827 427 160 2 143 566 122 548 0.0 563
Msa0457770 AT2G44500.2 63.415 410 121 6 11 411 17 406 3.39e-169 487
Msa0457770 AT5G64600.1 35.223 494 272 13 103 567 41 515 3.39e-80 261
Msa0457770 AT5G35570.1 36.175 434 249 10 151 569 223 643 9.10e-80 264
Msa0457770 AT1G22460.1 35.374 441 262 5 142 566 124 557 4.45e-79 260
Msa0457770 AT3G54100.1 35.023 434 254 9 152 570 195 615 7.03e-78 258
Msa0457770 AT1G20550.1 36.047 430 262 9 146 571 74 494 2.93e-77 255
Msa0457770 AT5G01100.1 34.651 430 261 8 152 569 190 611 4.84e-76 253
Msa0457770 AT1G76270.1 35.321 436 259 8 145 571 70 491 8.59e-76 251
Msa0457770 AT2G37980.1 34.033 429 265 8 152 570 199 619 8.58e-74 248
Msa0457770 AT2G03280.1 37.968 374 214 7 187 548 92 459 4.24e-72 239
Msa0457770 AT4G24530.1 34.549 466 236 11 149 566 72 516 6.84e-72 239
Msa0457770 AT5G15740.1 37.265 373 220 6 186 548 109 477 1.53e-71 238
Msa0457770 AT4G38390.1 34.703 438 261 9 145 571 87 510 2.09e-71 239
Msa0457770 AT5G65470.1 33.547 468 250 10 144 567 53 503 2.16e-69 233
Msa0457770 AT1G14020.1 35.500 400 237 8 187 571 95 488 3.09e-69 232
Msa0457770 AT3G02250.1 36.388 371 226 5 186 548 109 477 8.07e-69 231
Msa0457770 AT1G14970.2 33.486 436 262 8 149 566 7 432 1.27e-68 229
Msa0457770 AT1G14970.1 33.410 434 261 8 151 566 129 552 1.09e-66 227
Msa0457770 AT2G03280.2 35.411 401 214 8 187 548 92 486 1.89e-66 225
Msa0457770 AT4G16650.2 39.433 388 219 10 186 566 125 503 6.85e-66 224
Msa0457770 AT4G16650.1 39.433 388 219 10 186 566 125 503 6.85e-66 224
Msa0457770 AT1G14970.3 33.824 408 250 6 176 566 2 406 1.72e-65 219
Msa0457770 AT3G26370.1 34.673 398 240 9 186 566 161 555 1.28e-64 221
Msa0457770 AT1G38065.2 33.962 424 236 11 174 566 18 428 1.53e-63 216
Msa0457770 AT1G38065.1 33.981 412 229 10 186 566 39 438 2.35e-63 216
Msa0457770 AT2G01480.1 33.577 411 253 6 172 565 143 550 1.28e-62 216
Msa0457770 AT1G35510.1 31.626 449 280 9 137 568 120 558 1.54e-62 216
Msa0457770 AT1G38131.1 33.962 424 236 11 174 566 147 557 2.34e-62 216
Msa0457770 AT1G04910.1 30.140 428 272 11 152 566 61 474 9.74e-61 210
Msa0457770 AT2G01480.2 34.146 369 228 4 172 525 143 511 1.16e-57 202
Msa0457770 AT1G11990.1 29.038 520 322 15 87 567 78 589 2.13e-53 191
Msa0457770 AT1G62330.1 29.598 473 262 15 152 568 194 651 4.28e-53 192
Msa0457770 AT1G11990.2 27.656 546 318 15 87 567 78 611 3.14e-49 180
Msa0457770 AT1G29200.4 32.009 453 251 14 162 566 50 493 3.48e-47 172
Msa0457770 AT1G29200.1 32.009 453 251 14 162 566 50 493 3.48e-47 172
Msa0457770 AT1G29200.2 32.009 453 251 14 162 566 166 609 1.13e-46 173
Msa0457770 AT1G29200.3 32.319 427 233 13 188 566 1 419 1.30e-45 166
Msa0457770 AT3G30300.2 32.302 291 180 7 186 460 103 392 9.52e-34 136
Msa0457770 AT3G30300.1 32.302 291 180 7 186 460 103 392 9.52e-34 136
Msa0457770 AT1G52630.1 23.529 442 292 15 134 566 31 435 7.73e-30 122
Msa0457770 AT3G26370.2 35.714 182 103 4 186 354 161 341 9.62e-30 121
Msa0457770 AT3G03810.2 31.864 295 184 7 186 464 92 385 1.19e-29 124
Msa0457770 AT3G03810.1 31.864 295 184 7 186 464 92 385 1.19e-29 124
Msa0457770 AT1G52630.2 24.138 377 246 13 199 566 2 347 1.23e-25 108

Find 141 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTATGGTTGTTGTTTC+TGG 0.065594 3_3:+52901565 Msa0457770:CDS
AAGAAATTCAAGTGTTATTT+TGG 0.098612 3_3:+52904228 Msa0457770:three_prime_UTR
CTGTTTCTTGTTGGTTTCTT+TGG 0.136955 3_3:+52901175 Msa0457770:CDS
ACATCTAATCACCTTTATTT+TGG 0.173467 3_3:+52904402 Msa0457770:three_prime_UTR
TTAGGAAACTCTTGAATTAA+TGG 0.193953 3_3:-52903610 None:intergenic
AGTGAAGAATTTAGAAAATA+AGG 0.209967 3_3:-52904007 None:intergenic
GCCATCTCATGGAGGAAATA+TGG 0.218103 3_3:+52903747 Msa0457770:CDS
GTGGTTCTTGAAGGCTTCTT+TGG 0.222896 3_3:-52901115 None:intergenic
GTTGTTATTGCTAGGATTCT+TGG 0.223923 3_3:+52901625 Msa0457770:CDS
TAACTTCTTTGCATTGTTCT+TGG 0.233140 3_3:-52901020 None:intergenic
TTGACACTACTAATTGTTAT+TGG 0.260430 3_3:+52904466 Msa0457770:three_prime_UTR
CATTAACAAGATTCTGGTTT+AGG 0.277380 3_3:+52904184 Msa0457770:three_prime_UTR
TGATGTGGTTGCAGTGAATT+TGG 0.278147 3_3:+52902009 Msa0457770:intron
TTTGCAAGTCAATGTTATTT+GGG 0.282289 3_3:+52901669 Msa0457770:CDS
TGGAAGAAACCAAATGGTTT+AGG 0.301407 3_3:+52901469 Msa0457770:CDS
AAATATGGGCCGAGCGATTC+AGG 0.302214 3_3:+52903762 Msa0457770:CDS
TTCTTTGGTATGTTCAAGTT+TGG 0.303269 3_3:+52901190 Msa0457770:CDS
TTTACACATTAACAAGATTC+TGG 0.304952 3_3:+52904178 Msa0457770:three_prime_UTR
AGTTAGGTTTGATAGTTATA+TGG 0.317437 3_3:+52904598 Msa0457770:three_prime_UTR
CAGGGTCATAGGGCATTTAC+TGG 0.325806 3_3:+52903944 Msa0457770:intron
AACACTACCAAAACCAAGTT+TGG 0.332544 3_3:-52901296 None:intergenic
AAGCACTTCAATTTCCTTCC+AGG 0.337343 3_3:+52904280 Msa0457770:three_prime_UTR
GATCTCCCAGTCAAGAGTTC+TGG 0.338558 3_3:-52903079 None:intergenic
CATGTGCAATTTCAAGCAAA+TGG 0.350188 3_3:+52901415 Msa0457770:CDS
CTCAGAGACTATGTAATCTA+TGG 0.350975 3_3:-52903707 None:intergenic
ACTCCCATCAATACTTAGTT+AGG 0.360740 3_3:+52904582 Msa0457770:three_prime_UTR
TTTCTTGCTCTGTTTCTTGT+TGG 0.361638 3_3:+52901166 Msa0457770:CDS
TGTCGGTGCTTCAGGGTCAT+AGG 0.368740 3_3:+52903933 Msa0457770:intron
TGAGAACTTCTGAAGATTTG+TGG 0.370747 3_3:-52901251 None:intergenic
TAGGATTCTTGGTGCTGCTT+TGG 0.371467 3_3:+52901636 Msa0457770:CDS
GGAAGCATGTGTCTCTTGTT+TGG 0.373927 3_3:-52903986 None:intergenic
TTGAAGTGAAGAAGATGAAA+TGG 0.375169 3_3:-52901077 None:intergenic
CAAACCTAACTAAGTATTGA+TGG 0.385611 3_3:-52904586 None:intergenic
ACGTGTCACTGTTGTGTGTC+TGG 0.386502 3_3:+52904494 Msa0457770:three_prime_UTR
CTCTTGAATTAATGGAAGTA+AGG 0.387396 3_3:-52903602 None:intergenic
TCAACAGAAAATCAACACTA+TGG 0.387562 3_3:+52901143 Msa0457770:CDS
CCTTGCTCTTCATTTAAGAA+TGG 0.389315 3_3:+52902970 Msa0457770:CDS
GAGAATTACAACCATTTGTA+AGG 0.389523 3_3:+52903665 Msa0457770:CDS
TTGAAGATGGTGGTTCTTGA+AGG 0.391892 3_3:-52901124 None:intergenic
CTTCTAATTGAAGACATGTT+TGG 0.394301 3_3:+52904344 Msa0457770:three_prime_UTR
GGTTTATATCCTAAACCATT+TGG 0.395737 3_3:-52901478 None:intergenic
ATAACATTGACTTGCAAAAC+AGG 0.403737 3_3:-52901664 None:intergenic
CTTGTCATTATGTGTGTTGA+TGG 0.406673 3_3:-52902099 None:intergenic
ATCTTGTTAATGTGTAAATC+AGG 0.412746 3_3:-52904173 None:intergenic
TTTATACAAAGTGAATGTAT+AGG 0.415577 3_3:+52904659 Msa0457770:three_prime_UTR
GAATTGCACCAAGACTCATT+AGG 0.415792 3_3:+52904061 Msa0457770:CDS
GTGAGGACAGGTTGTCTCCC+TGG 0.417494 3_3:+52903007 Msa0457770:CDS
TGTAAGGAAAGCTTCTCTAA+TGG 0.420479 3_3:+52903681 Msa0457770:CDS
AATTGCACCAAGACTCATTA+GGG 0.424630 3_3:+52904062 Msa0457770:CDS
ACACGTAGTCACCAAAATAA+AGG 0.427153 3_3:-52904413 None:intergenic
TCTCTGAAGAAGAGTTCAAT+AGG 0.438361 3_3:+52904029 Msa0457770:CDS
AGAGAAGCTTTCCTTACAAA+TGG 0.441636 3_3:-52903676 None:intergenic
GGATAGAAGAAAGTATCTTA+TGG 0.445638 3_3:+52901552 Msa0457770:CDS
TGAATGTTATCGAAGTTATG+AGG 0.455620 3_3:+52903146 Msa0457770:CDS
TGAATTTCTTGTTCAGTGGT+AGG 0.463487 3_3:-52904215 None:intergenic
GCACTCAGGGACAGGATACT+TGG 0.464829 3_3:-52904125 None:intergenic
TTCATTATTATGAATACTTG+TGG 0.467258 3_3:+52904717 Msa0457770:three_prime_UTR
TGGGCTGGAGGGAAACCATT+GGG 0.467264 3_3:+52903568 Msa0457770:CDS
TTTGAGGAAATGAGGTATCA+GGG 0.468647 3_3:-52901219 None:intergenic
AAAGATGTTTGGGTGAGGAC+AGG 0.469149 3_3:+52902995 Msa0457770:CDS
TTGTTACCGAGTTCCTCAAT+TGG 0.474750 3_3:-52902914 None:intergenic
CTTATGGTTGTTGTTTCTGG+AGG 0.476782 3_3:+52901568 Msa0457770:CDS
AAGGAAGATCTTGCCTTGCC+AGG 0.480516 3_3:+52903643 Msa0457770:CDS
TTCTGAAGATTTGTGGAGCA+TGG 0.482167 3_3:-52901244 None:intergenic
ACAAATGGTTGTAATTCTCC+TGG 0.488364 3_3:-52903661 None:intergenic
CTTGGATTCAAGGCTTTCAA+AGG 0.489677 3_3:+52902597 Msa0457770:CDS
CCATCTCATGGAGGAAATAT+GGG 0.491947 3_3:+52903748 Msa0457770:CDS
AGTAGTGTCAATGCCGGACT+CGG 0.493725 3_3:-52904454 None:intergenic
AAGATTTGCTGAGCCAATTG+AGG 0.493764 3_3:+52902901 Msa0457770:CDS
CTGGCTGCCCTAATGAGTCT+TGG 0.495915 3_3:-52904069 None:intergenic
CAAAGACCAGAACTCTTGAC+TGG 0.496719 3_3:+52903073 Msa0457770:CDS
CACTTCATGCCACTCCGAGT+TGG 0.500852 3_3:+52902142 Msa0457770:CDS
CATTAGGGCAGCCAGAGCTT+AGG 0.507074 3_3:+52904077 Msa0457770:CDS
ATTATATGAGAGAAAGTGAA+AGG 0.513739 3_3:+52901521 Msa0457770:CDS
ATGTGCAATTTCAAGCAAAT+GGG 0.513883 3_3:+52901416 Msa0457770:CDS
GTCGGTGCTTCAGGGTCATA+GGG 0.514514 3_3:+52903934 Msa0457770:intron
ATGTTATTTGGGGTGATGAA+AGG 0.517325 3_3:+52901680 Msa0457770:CDS
TTATGGTTGTTGTTTCTGGA+GGG 0.518110 3_3:+52901569 Msa0457770:CDS
AGCACTTCAATTTCCTTCCA+GGG 0.518471 3_3:+52904281 Msa0457770:three_prime_UTR
GAATTTCTTGTTCAGTGGTA+GGG 0.519084 3_3:-52904214 None:intergenic
TTGGGCTGGAGGGAAACCAT+TGG 0.519413 3_3:+52903567 Msa0457770:CDS
CAAGAACAAGAACAAGAAAG+AGG 0.524252 3_3:+52901376 Msa0457770:CDS
AGTGGAGGCCTTCCTTCCAC+TGG 0.524717 3_3:-52902123 None:intergenic
ATGGAGTTTATGATTTGTGA+TGG 0.526571 3_3:-52901058 None:intergenic
TCACATTCTCTGAAACTCAA+TGG 0.529041 3_3:+52900991 Msa0457770:exon
TTTAGGTTACTGAAAGCTCT+AGG 0.530891 3_3:+52903520 Msa0457770:intron
TTTCAAGCAAATGGGTCTAG+TGG 0.532896 3_3:+52901424 Msa0457770:CDS
TTGCACATGCACTCAGGGAC+AGG 0.536508 3_3:-52904133 None:intergenic
TCTTCAGAAGCTTCGTTGCA+AGG 0.536611 3_3:+52902633 Msa0457770:CDS
GCAACGAAGCTTCTGAAGAT+CGG 0.539163 3_3:-52902629 None:intergenic
TCGTGTCTAGTGTCCGAGTC+CGG 0.542303 3_3:+52904441 Msa0457770:three_prime_UTR
TTGATGCTGTTGTTATTGCT+AGG 0.555554 3_3:+52901617 Msa0457770:CDS
CTCTTCTGAGATATCTTGCA+CGG 0.560668 3_3:-52902167 None:intergenic
ATAACTTCGATAACATTCAA+AGG 0.562246 3_3:-52903142 None:intergenic
CCCATATTTCCTCCATGAGA+TGG 0.562723 3_3:-52903748 None:intergenic
TGACAAGGCCAGTGGAAGGA+AGG 0.566227 3_3:+52902115 Msa0457770:CDS
AAACCTAACTAAGTATTGAT+GGG 0.566898 3_3:-52904585 None:intergenic
ATTGATGGGAGTACACCTGC+AGG 0.567644 3_3:-52904571 None:intergenic
CGAAGCTTCTGAAGATCGGA+AGG 0.568676 3_3:-52902625 None:intergenic
CTTAAATGAAGAGCAAGGTA+AGG 0.569568 3_3:-52902965 None:intergenic
GAATCATTGCACATGCACTC+AGG 0.572075 3_3:-52904139 None:intergenic
GAAATTGAAGTGCTTCCAAA+GGG 0.572256 3_3:-52904272 None:intergenic
TTAATAACCTCATCATACTC+AGG 0.575577 3_3:-52903037 None:intergenic
CTCGGAGTGGCATGAAGTGG+AGG 0.577955 3_3:-52902138 None:intergenic
TACCGGTGTCATGTCCGTCT+CGG 0.579238 3_3:-52904370 None:intergenic
AATCATTGCACATGCACTCA+GGG 0.579973 3_3:-52904138 None:intergenic
GGAAATTGAAGTGCTTCCAA+AGG 0.583777 3_3:-52904273 None:intergenic
CACTTGAATTTCTTGTTCAG+TGG 0.585738 3_3:-52904219 None:intergenic
CAACTCGGAGTGGCATGAAG+TGG 0.586354 3_3:-52902141 None:intergenic
CCATTCTTAAATGAAGAGCA+AGG 0.589706 3_3:-52902970 None:intergenic
CCACTCTCACATCATTTGCA+AGG 0.590073 3_3:-52902065 None:intergenic
TAGAAGTGTCGATGCTACAT+AGG 0.593043 3_3:-52904327 None:intergenic
GGGATGTATGTGTAAAAGAA+TGG 0.596514 3_3:-52904550 None:intergenic
TGGTTGTAATTCTCCTGGCA+AGG 0.596530 3_3:-52903656 None:intergenic
ACAATTAGTAGTGTCAATGC+CGG 0.597273 3_3:-52904460 None:intergenic
ACAATCATACCTGAATCGCT+CGG 0.599045 3_3:-52903771 None:intergenic
TTATATGAGAGAAAGTGAAA+GGG 0.602787 3_3:+52901522 Msa0457770:CDS
TCATACTCAGGAGTTAGACC+AGG 0.603634 3_3:-52903025 None:intergenic
TGTTAATGTGTAAATCAGGA+TGG 0.605044 3_3:-52904169 None:intergenic
GCACTTCAATTTCCTTCCAG+GGG 0.606283 3_3:+52904282 Msa0457770:three_prime_UTR
TTAGATGTAATAAAATGTAC+CGG 0.612293 3_3:-52904387 None:intergenic
CACTTCAATTTCCTTCCAGG+GGG 0.614929 3_3:+52904283 Msa0457770:three_prime_UTR
GCAGAGAGAATGAAAAGCAA+AGG 0.616583 3_3:+52902941 Msa0457770:CDS
TATCTTGCACGGATCCAACT+CGG 0.616793 3_3:-52902156 None:intergenic
GACATGTTTGGTGTCCGAGA+CGG 0.620081 3_3:+52904356 Msa0457770:three_prime_UTR
ACTATGCAGTTCAATAGAGA+AGG 0.620483 3_3:+52902556 Msa0457770:intron
GCTGAGCCAATTGAGGAACT+CGG 0.621180 3_3:+52902908 Msa0457770:CDS
CCTTGCAAATGATGTGAGAG+TGG 0.638538 3_3:+52902065 Msa0457770:CDS
ATAATGACAAGGCCAGTGGA+AGG 0.661399 3_3:+52902111 Msa0457770:CDS
ACACATAATGACAAGGCCAG+TGG 0.663500 3_3:+52902107 Msa0457770:CDS
TTGCAAGTCAATGTTATTTG+GGG 0.663997 3_3:+52901670 Msa0457770:CDS
TCTAACTCCTGAGTATGATG+AGG 0.664165 3_3:+52903030 Msa0457770:CDS
GTCCGAGACGGACATGACAC+CGG 0.665091 3_3:+52904368 Msa0457770:three_prime_UTR
TCATTACTAAAACTCAAACA+AGG 0.670567 3_3:-52901499 None:intergenic
TGCACGGATCCAACTCGGAG+TGG 0.677266 3_3:-52902151 None:intergenic
TTGATGGGAGTACACCTGCA+GGG 0.679548 3_3:-52904570 None:intergenic
AAAGACCAGAACTCTTGACT+GGG 0.680016 3_3:+52903074 Msa0457770:CDS
CATCAACACACATAATGACA+AGG 0.683592 3_3:+52902100 Msa0457770:CDS
CTGGAGGGATGAATCAACAG+AGG 0.700723 3_3:+52901584 Msa0457770:CDS
GCTGGAGGGAAACCATTGGG+TGG 0.722830 3_3:+52903571 Msa0457770:CDS
CATACTCAGGAGTTAGACCA+GGG 0.725761 3_3:-52903024 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTATTTATTACTTATAATT+TGG + chr3_3:52901859-52901878 Msa0457770:intron 10.0%
!!! ATTCTATTTTCTCAAATTAT+TGG - chr3_3:52902342-52902361 None:intergenic 15.0%
!! CATATAATCAAATTTCTAAA+GGG - chr3_3:52902821-52902840 None:intergenic 15.0%
!! TCATATAATCAAATTTCTAA+AGG - chr3_3:52902822-52902841 None:intergenic 15.0%
!! TAATAACTATATTCTAATAG+CGG - chr3_3:52903431-52903450 None:intergenic 15.0%
!!! TATAGTTATTATGTTTTATG+TGG + chr3_3:52903440-52903459 Msa0457770:intron 15.0%
!! AAAAGATGCAAAAATTTATT+GGG + chr3_3:52903549-52903568 Msa0457770:CDS 15.0%
!!! TTTATGTTTTTTCATTGATG+TGG + chr3_3:52901994-52902013 Msa0457770:intron 20.0%
!! GATTGTAACAATATATGTTT+CGG + chr3_3:52902366-52902385 Msa0457770:intron 20.0%
!! ATCATACATAATGAGAAAAA+GGG - chr3_3:52902522-52902541 None:intergenic 20.0%
!! TATCATACATAATGAGAAAA+AGG - chr3_3:52902523-52902542 None:intergenic 20.0%
!!! ATGTTTTTGGTTTCATATTT+AGG + chr3_3:52903503-52903522 Msa0457770:intron 20.0%
!!! CAATAAATTTTTGCATCTTT+TGG - chr3_3:52903550-52903569 None:intergenic 20.0%
!! CAAAAGATGCAAAAATTTAT+TGG + chr3_3:52903548-52903567 Msa0457770:CDS 20.0%
!!! GTTTCCTAATTTTTACAATA+AGG + chr3_3:52903624-52903643 Msa0457770:CDS 20.0%
!! TCTTCCTTATTGTAAAAATT+AGG - chr3_3:52903631-52903650 None:intergenic 20.0%
!! AGTGAAGAATTTAGAAAATA+AGG - chr3_3:52904010-52904029 None:intergenic 20.0%
!!! AAGAAATTCAAGTGTTATTT+TGG + chr3_3:52904228-52904247 Msa0457770:three_prime_UTR 20.0%
!! TTAGATGTAATAAAATGTAC+CGG - chr3_3:52904390-52904409 None:intergenic 20.0%
!! TTTATACAAAGTGAATGTAT+AGG + chr3_3:52904659-52904678 Msa0457770:three_prime_UTR 20.0%
!! TTCATTATTATGAATACTTG+TGG + chr3_3:52904717-52904736 Msa0457770:three_prime_UTR 20.0%
! TCATTACTAAAACTCAAACA+AGG - chr3_3:52901502-52901521 None:intergenic 25.0%
! ATTATATGAGAGAAAGTGAA+AGG + chr3_3:52901521-52901540 Msa0457770:CDS 25.0%
! TTATATGAGAGAAAGTGAAA+GGG + chr3_3:52901522-52901541 Msa0457770:CDS 25.0%
!! TTTTGCAAGTCAATGTTATT+TGG + chr3_3:52901668-52901687 Msa0457770:CDS 25.0%
!! TTTGCAAGTCAATGTTATTT+GGG + chr3_3:52901669-52901688 Msa0457770:CDS 25.0%
! CTATTACAAGGATTTAACAT+AGG - chr3_3:52901948-52901967 None:intergenic 25.0%
! CAAAATTCTCTTCTATTACA+AGG - chr3_3:52901960-52901979 None:intergenic 25.0%
! ACATCTGATTATATTAACTC+TGG - chr3_3:52902465-52902484 None:intergenic 25.0%
!! AAGTTGTCATCTAAATTTTC+AGG + chr3_3:52902862-52902881 Msa0457770:intron 25.0%
! TAAGAATGGAAAAAGATGTT+TGG + chr3_3:52902984-52903003 Msa0457770:CDS 25.0%
! AAGAATGGAAAAAGATGTTT+GGG + chr3_3:52902985-52903004 Msa0457770:CDS 25.0%
! ATAACTTCGATAACATTCAA+AGG - chr3_3:52903145-52903164 None:intergenic 25.0%
! ATAAGTGACCTATTGTAAAA+GGG - chr3_3:52903238-52903257 None:intergenic 25.0%
!! AAGATTTCATGAGATGTTTT+TGG + chr3_3:52903490-52903509 Msa0457770:intron 25.0%
! TTAGGAAACTCTTGAATTAA+TGG - chr3_3:52903613-52903632 None:intergenic 25.0%
! ATCTTGTTAATGTGTAAATC+AGG - chr3_3:52904176-52904195 None:intergenic 25.0%
! TTTACACATTAACAAGATTC+TGG + chr3_3:52904178-52904197 Msa0457770:three_prime_UTR 25.0%
!!! AATTCAAGTGTTATTTTGGA+TGG + chr3_3:52904232-52904251 Msa0457770:three_prime_UTR 25.0%
!!! ACATCTAATCACCTTTATTT+TGG + chr3_3:52904402-52904421 Msa0457770:three_prime_UTR 25.0%
! TTGACACTACTAATTGTTAT+TGG + chr3_3:52904466-52904485 Msa0457770:three_prime_UTR 25.0%
!!! AAACCTAACTAAGTATTGAT+GGG - chr3_3:52904588-52904607 None:intergenic 25.0%
!! AGTTAGGTTTGATAGTTATA+TGG + chr3_3:52904598-52904617 Msa0457770:three_prime_UTR 25.0%
TAACTTCTTTGCATTGTTCT+TGG - chr3_3:52901023-52901042 None:intergenic 30.0%
! ATGGAGTTTATGATTTGTGA+TGG - chr3_3:52901061-52901080 None:intergenic 30.0%
TTGAAGTGAAGAAGATGAAA+TGG - chr3_3:52901080-52901099 None:intergenic 30.0%
!!! TGTTGATTTTCTGTTGAAGA+TGG - chr3_3:52901140-52901159 None:intergenic 30.0%
TCAACAGAAAATCAACACTA+TGG + chr3_3:52901143-52901162 Msa0457770:CDS 30.0%
! TTCTTTGGTATGTTCAAGTT+TGG + chr3_3:52901190-52901209 Msa0457770:CDS 30.0%
! CATCATTCATTTTCCAAACT+TGG + chr3_3:52901283-52901302 Msa0457770:CDS 30.0%
! TCATTTTCCAAACTTGGTTT+TGG + chr3_3:52901289-52901308 Msa0457770:CDS 30.0%
ATGTGCAATTTCAAGCAAAT+GGG + chr3_3:52901416-52901435 Msa0457770:CDS 30.0%
! ATGAGAAAAAGAGTGAGTTT+TGG + chr3_3:52901449-52901468 Msa0457770:CDS 30.0%
GGTTTATATCCTAAACCATT+TGG - chr3_3:52901481-52901500 None:intergenic 30.0%
!! GGATAGAAGAAAGTATCTTA+TGG + chr3_3:52901552-52901571 Msa0457770:CDS 30.0%
!! TATCTTATGGTTGTTGTTTC+TGG + chr3_3:52901565-52901584 Msa0457770:CDS 30.0%
!! ATAACATTGACTTGCAAAAC+AGG - chr3_3:52901667-52901686 None:intergenic 30.0%
! TTGCAAGTCAATGTTATTTG+GGG + chr3_3:52901670-52901689 Msa0457770:CDS 30.0%
!! AAGAATAGTTCACTGCTTTT+TGG + chr3_3:52902191-52902210 Msa0457770:intron 30.0%
!! AATCTGAAGTTTTACTGCAT+AGG + chr3_3:52902423-52902442 Msa0457770:intron 30.0%
ACAGAAATTGATAGGTACAA+GGG - chr3_3:52902490-52902509 None:intergenic 30.0%
AACAGAAATTGATAGGTACA+AGG - chr3_3:52902491-52902510 None:intergenic 30.0%
CTTGTTCAACAGAAATTGAT+AGG - chr3_3:52902498-52902517 None:intergenic 30.0%
TTAATAACCTCATCATACTC+AGG - chr3_3:52903040-52903059 None:intergenic 30.0%
GACTTACCATCAAAGAAAAA+TGG + chr3_3:52903108-52903127 Msa0457770:CDS 30.0%
TGAATGTTATCGAAGTTATG+AGG + chr3_3:52903146-52903165 Msa0457770:CDS 30.0%
ATTGCTAAAGAGACCTATTT+AGG + chr3_3:52903210-52903229 Msa0457770:intron 30.0%
CATAAGTGACCTATTGTAAA+AGG - chr3_3:52903239-52903258 None:intergenic 30.0%
CTCTTGAATTAATGGAAGTA+AGG - chr3_3:52903605-52903624 None:intergenic 30.0%
GAGAATTACAACCATTTGTA+AGG + chr3_3:52903665-52903684 Msa0457770:CDS 30.0%
AGCTTAGGACTAACAAAAAT+GGG + chr3_3:52904092-52904111 Msa0457770:CDS 30.0%
TGTTAATGTGTAAATCAGGA+TGG - chr3_3:52904172-52904191 None:intergenic 30.0%
!! CATTAACAAGATTCTGGTTT+AGG + chr3_3:52904184-52904203 Msa0457770:three_prime_UTR 30.0%
CTTCTAATTGAAGACATGTT+TGG + chr3_3:52904344-52904363 Msa0457770:three_prime_UTR 30.0%
!! CAAACCTAACTAAGTATTGA+TGG - chr3_3:52904589-52904608 None:intergenic 30.0%
!! TCACATTCTCTGAAACTCAA+TGG + chr3_3:52900991-52901010 Msa0457770:exon 35.0%
! TGATTTTCTGTTGAAGATGG+TGG - chr3_3:52901137-52901156 None:intergenic 35.0%
! TTTCTTGCTCTGTTTCTTGT+TGG + chr3_3:52901166-52901185 Msa0457770:CDS 35.0%
!! CTGTTTCTTGTTGGTTTCTT+TGG + chr3_3:52901175-52901194 Msa0457770:CDS 35.0%
! TTTGAGGAAATGAGGTATCA+GGG - chr3_3:52901222-52901241 None:intergenic 35.0%
!! TTTTGAGGAAATGAGGTATC+AGG - chr3_3:52901223-52901242 None:intergenic 35.0%
! TGAGAACTTCTGAAGATTTG+TGG - chr3_3:52901254-52901273 None:intergenic 35.0%
AACACTACCAAAACCAAGTT+TGG - chr3_3:52901299-52901318 None:intergenic 35.0%
CTTGCAAAAACTCGTCATTA+TGG - chr3_3:52901360-52901379 None:intergenic 35.0%
CAAGAACAAGAACAAGAAAG+AGG + chr3_3:52901376-52901395 Msa0457770:CDS 35.0%
! ACAAGAAAGAGGAGAGTTTT+TGG + chr3_3:52901387-52901406 Msa0457770:CDS 35.0%
CATGTGCAATTTCAAGCAAA+TGG + chr3_3:52901415-52901434 Msa0457770:CDS 35.0%
!!! GAGTTTTGGAAGAAACCAAA+TGG + chr3_3:52901463-52901482 Msa0457770:CDS 35.0%
TGGAAGAAACCAAATGGTTT+AGG + chr3_3:52901469-52901488 Msa0457770:CDS 35.0%
!! AGAAAGTGAAAGGGTTTTGA+AGG + chr3_3:52901531-52901550 Msa0457770:CDS 35.0%
!! TTATGGTTGTTGTTTCTGGA+GGG + chr3_3:52901569-52901588 Msa0457770:CDS 35.0%
! TTGATGCTGTTGTTATTGCT+AGG + chr3_3:52901617-52901636 Msa0457770:CDS 35.0%
! GTTGTTATTGCTAGGATTCT+TGG + chr3_3:52901625-52901644 Msa0457770:CDS 35.0%
! ATGTTATTTGGGGTGATGAA+AGG + chr3_3:52901680-52901699 Msa0457770:CDS 35.0%
CTTGTCATTATGTGTGTTGA+TGG - chr3_3:52902102-52902121 None:intergenic 35.0%
CATCAACACACATAATGACA+AGG + chr3_3:52902100-52902119 Msa0457770:CDS 35.0%
GGACTCAAAATTATTCACAC+AGG - chr3_3:52902231-52902250 None:intergenic 35.0%
ATTCCACAAATGTACACTGT+AGG - chr3_3:52902252-52902271 None:intergenic 35.0%
!! ATAGACGTCATTTTCAGACA+AGG + chr3_3:52902278-52902297 Msa0457770:intron 35.0%
! TCATTTTCAGACAAGGTCAT+CGG + chr3_3:52902285-52902304 Msa0457770:intron 35.0%
TTGAACTGCATAGTACAACA+AGG - chr3_3:52902550-52902569 None:intergenic 35.0%
ACTATGCAGTTCAATAGAGA+AGG + chr3_3:52902556-52902575 Msa0457770:intron 35.0%
! TTAGCTCTTAACATACCACA+AGG + chr3_3:52902769-52902788 Msa0457770:intron 35.0%
! GTTTTCTTGGATTTTCCTTG+TGG - chr3_3:52902787-52902806 None:intergenic 35.0%
!! GGTCTTTTCAGTTGTTTTCT+TGG - chr3_3:52902800-52902819 None:intergenic 35.0%
CTTAAATGAAGAGCAAGGTA+AGG - chr3_3:52902968-52902987 None:intergenic 35.0%
CCATTCTTAAATGAAGAGCA+AGG - chr3_3:52902973-52902992 None:intergenic 35.0%
CCTTGCTCTTCATTTAAGAA+TGG + chr3_3:52902970-52902989 Msa0457770:CDS 35.0%
TACCATCAAAGAAAAATGGC+TGG + chr3_3:52903112-52903131 Msa0457770:CDS 35.0%
TTGTAAAAGGGAGCCTAAAT+AGG - chr3_3:52903226-52903245 None:intergenic 35.0%
! TTTAGGCTCCCTTTTACAAT+AGG + chr3_3:52903227-52903246 Msa0457770:intron 35.0%
! TTTAGGTTACTGAAAGCTCT+AGG + chr3_3:52903520-52903539 Msa0457770:intron 35.0%
GATGCAAAAATTTATTGGGC+TGG + chr3_3:52903553-52903572 Msa0457770:CDS 35.0%
! CAAAAATTTATTGGGCTGGA+GGG + chr3_3:52903557-52903576 Msa0457770:CDS 35.0%
ACAAATGGTTGTAATTCTCC+TGG - chr3_3:52903664-52903683 None:intergenic 35.0%
AGAGAAGCTTTCCTTACAAA+TGG - chr3_3:52903679-52903698 None:intergenic 35.0%
TGTAAGGAAAGCTTCTCTAA+TGG + chr3_3:52903681-52903700 Msa0457770:CDS 35.0%
CTCAGAGACTATGTAATCTA+TGG - chr3_3:52903710-52903729 None:intergenic 35.0%
TATGATTGTCAAGTTCGACA+AGG + chr3_3:52903785-52903804 Msa0457770:intron 35.0%
! GTCTGATGATTTTGTCTTGT+CGG + chr3_3:52903916-52903935 Msa0457770:intron 35.0%
! TCTCTGAAGAAGAGTTCAAT+AGG + chr3_3:52904029-52904048 Msa0457770:CDS 35.0%
AATTGCACCAAGACTCATTA+GGG + chr3_3:52904062-52904081 Msa0457770:CDS 35.0%
! TTTTTGTTAGTCCTAAGCTC+TGG - chr3_3:52904091-52904110 None:intergenic 35.0%
GAGCTTAGGACTAACAAAAA+TGG + chr3_3:52904091-52904110 Msa0457770:CDS 35.0%
GAATTTCTTGTTCAGTGGTA+GGG - chr3_3:52904217-52904236 None:intergenic 35.0%
TGAATTTCTTGTTCAGTGGT+AGG - chr3_3:52904218-52904237 None:intergenic 35.0%
CACTTGAATTTCTTGTTCAG+TGG - chr3_3:52904222-52904241 None:intergenic 35.0%
CTTCCAAAGGGATCAAAAAA+TGG - chr3_3:52904263-52904282 None:intergenic 35.0%
! GAAATTGAAGTGCTTCCAAA+GGG - chr3_3:52904275-52904294 None:intergenic 35.0%
ACACGTAGTCACCAAAATAA+AGG - chr3_3:52904416-52904435 None:intergenic 35.0%
ACAATTAGTAGTGTCAATGC+CGG - chr3_3:52904463-52904482 None:intergenic 35.0%
GGGATGTATGTGTAAAAGAA+TGG - chr3_3:52904553-52904572 None:intergenic 35.0%
ACTCCCATCAATACTTAGTT+AGG + chr3_3:52904582-52904601 Msa0457770:three_prime_UTR 35.0%
! TTGAAGATGGTGGTTCTTGA+AGG - chr3_3:52901127-52901146 None:intergenic 40.0%
!! GCATGGTTTTTGAGGAAATG+AGG - chr3_3:52901230-52901249 None:intergenic 40.0%
! TTTGTGGAGCATGGTTTTTG+AGG - chr3_3:52901238-52901257 None:intergenic 40.0%
! TTCTGAAGATTTGTGGAGCA+TGG - chr3_3:52901247-52901266 None:intergenic 40.0%
TTTCAAGCAAATGGGTCTAG+TGG + chr3_3:52901424-52901443 Msa0457770:CDS 40.0%
!! CTTATGGTTGTTGTTTCTGG+AGG + chr3_3:52901568-52901587 Msa0457770:CDS 40.0%
! CTTCACATTGAAAGCGTGTT+TGG + chr3_3:52901791-52901810 Msa0457770:intron 40.0%
!!! GTTTGGTGTCTGACATGTTT+TGG + chr3_3:52901808-52901827 Msa0457770:intron 40.0%
TGATGTGGTTGCAGTGAATT+TGG + chr3_3:52902009-52902028 Msa0457770:intron 40.0%
!! CTCTTCTGAGATATCTTGCA+CGG - chr3_3:52902170-52902189 None:intergenic 40.0%
AGTCCTACAGTGTACATTTG+TGG + chr3_3:52902246-52902265 Msa0457770:intron 40.0%
TAATCTCGACGTGTCAATGT+CGG - chr3_3:52902319-52902338 None:intergenic 40.0%
CTTCAGATTGAAGACGTGTT+TGG - chr3_3:52902412-52902431 None:intergenic 40.0%
!!! GAAGGTGTTTTGCTTTTACG+CGG + chr3_3:52902574-52902593 Msa0457770:CDS 40.0%
CTTGGATTCAAGGCTTTCAA+AGG + chr3_3:52902597-52902616 Msa0457770:CDS 40.0%
! ATGCTATTTGTCAGAACACG+AGG + chr3_3:52902707-52902726 Msa0457770:intron 40.0%
!! AAGATTTGCTGAGCCAATTG+AGG + chr3_3:52902901-52902920 Msa0457770:CDS 40.0%
TTGTTACCGAGTTCCTCAAT+TGG - chr3_3:52902917-52902936 None:intergenic 40.0%
GCAGAGAGAATGAAAAGCAA+AGG + chr3_3:52902941-52902960 Msa0457770:CDS 40.0%
TGGAAAAAGATGTTTGGGTG+AGG + chr3_3:52902990-52903009 Msa0457770:CDS 40.0%
TCTAACTCCTGAGTATGATG+AGG + chr3_3:52903030-52903049 Msa0457770:CDS 40.0%
AAAGACCAGAACTCTTGACT+GGG + chr3_3:52903074-52903093 Msa0457770:CDS 40.0%
!!! GTCCAGCCATTTTTCTTTGA+TGG - chr3_3:52903117-52903136 None:intergenic 40.0%
!! TCACGAGTTGATGTCAAACA+CGG + chr3_3:52903280-52903299 Msa0457770:intron 40.0%
ACACAAGACATGACATGACA+CGG - chr3_3:52903383-52903402 None:intergenic 40.0%
AAAGACATGAAGCACGAACA+GGG - chr3_3:52903406-52903425 None:intergenic 40.0%
! GCAAAAATTTATTGGGCTGG+AGG + chr3_3:52903556-52903575 Msa0457770:CDS 40.0%
! GATGTTTTCATGCCATCTCA+TGG + chr3_3:52903736-52903755 Msa0457770:CDS 40.0%
CCATCTCATGGAGGAAATAT+GGG + chr3_3:52903748-52903767 Msa0457770:CDS 40.0%
ACAATCATACCTGAATCGCT+CGG - chr3_3:52903774-52903793 None:intergenic 40.0%
TGTCAGATACTAACACGAGT+TGG - chr3_3:52903825-52903844 None:intergenic 40.0%
GAATTGCACCAAGACTCATT+AGG + chr3_3:52904061-52904080 Msa0457770:CDS 40.0%
AATCATTGCACATGCACTCA+GGG - chr3_3:52904141-52904160 None:intergenic 40.0%
!!! GCTCCATTTTTTGATCCCTT+TGG + chr3_3:52904257-52904276 Msa0457770:three_prime_UTR 40.0%
! GGAAATTGAAGTGCTTCCAA+AGG - chr3_3:52904276-52904295 None:intergenic 40.0%
AAGCACTTCAATTTCCTTCC+AGG + chr3_3:52904280-52904299 Msa0457770:three_prime_UTR 40.0%
AGCACTTCAATTTCCTTCCA+GGG + chr3_3:52904281-52904300 Msa0457770:three_prime_UTR 40.0%
!! TAGAAGTGTCGATGCTACAT+AGG - chr3_3:52904330-52904349 None:intergenic 40.0%
! TTTTACACATACATCCCTGC+AGG + chr3_3:52904556-52904575 Msa0457770:three_prime_UTR 40.0%
GTGGTTCTTGAAGGCTTCTT+TGG - chr3_3:52901118-52901137 None:intergenic 45.0%
!! TAGGATTCTTGGTGCTGCTT+TGG + chr3_3:52901636-52901655 Msa0457770:CDS 45.0%
CCACTCTCACATCATTTGCA+AGG - chr3_3:52902068-52902087 None:intergenic 45.0%
CCTTGCAAATGATGTGAGAG+TGG + chr3_3:52902065-52902084 Msa0457770:CDS 45.0%
ACACATAATGACAAGGCCAG+TGG + chr3_3:52902107-52902126 Msa0457770:CDS 45.0%
ATAATGACAAGGCCAGTGGA+AGG + chr3_3:52902111-52902130 Msa0457770:CDS 45.0%
TATCTTGCACGGATCCAACT+CGG - chr3_3:52902159-52902178 None:intergenic 45.0%
!! TGTTTTGCTTTTACGCGGCT+TGG + chr3_3:52902579-52902598 Msa0457770:CDS 45.0%
!! TTTTACGCGGCTTGGATTCA+AGG + chr3_3:52902587-52902606 Msa0457770:CDS 45.0%
! GCAACGAAGCTTCTGAAGAT+CGG - chr3_3:52902632-52902651 None:intergenic 45.0%
TCTTCAGAAGCTTCGTTGCA+AGG + chr3_3:52902633-52902652 Msa0457770:CDS 45.0%
AAAGATGTTTGGGTGAGGAC+AGG + chr3_3:52902995-52903014 Msa0457770:CDS 45.0%
CATACTCAGGAGTTAGACCA+GGG - chr3_3:52903027-52903046 None:intergenic 45.0%
TCATACTCAGGAGTTAGACC+AGG - chr3_3:52903028-52903047 None:intergenic 45.0%
CAAAGACCAGAACTCTTGAC+TGG + chr3_3:52903073-52903092 Msa0457770:CDS 45.0%
!! TCACTTATGTAGCACCGATG+TGG + chr3_3:52903250-52903269 Msa0457770:intron 45.0%
!! CACTTATGTAGCACCGATGT+GGG + chr3_3:52903251-52903270 Msa0457770:intron 45.0%
CAAAGACATGAAGCACGAAC+AGG - chr3_3:52903407-52903426 None:intergenic 45.0%
! AAGGCTTCTTTTCCACCCAA+TGG - chr3_3:52903586-52903605 None:intergenic 45.0%
TGGTTGTAATTCTCCTGGCA+AGG - chr3_3:52903659-52903678 None:intergenic 45.0%
! GTTTTCATGCCATCTCATGG+AGG + chr3_3:52903739-52903758 Msa0457770:CDS 45.0%
GCCATCTCATGGAGGAAATA+TGG + chr3_3:52903747-52903766 Msa0457770:CDS 45.0%
CCCATATTTCCTCCATGAGA+TGG - chr3_3:52903751-52903770 None:intergenic 45.0%
!! TTTTGTCTTGTCGGTGCTTC+AGG + chr3_3:52903925-52903944 Msa0457770:intron 45.0%
! TTTGTCTTGTCGGTGCTTCA+GGG + chr3_3:52903926-52903945 Msa0457770:intron 45.0%
! GGAAGCATGTGTCTCTTGTT+TGG - chr3_3:52903989-52904008 None:intergenic 45.0%
GAATCATTGCACATGCACTC+AGG - chr3_3:52904142-52904161 None:intergenic 45.0%
GCACTTCAATTTCCTTCCAG+GGG + chr3_3:52904282-52904301 Msa0457770:three_prime_UTR 45.0%
CACTTCAATTTCCTTCCAGG+GGG + chr3_3:52904283-52904302 Msa0457770:three_prime_UTR 45.0%
TCTTATAGTTTCCCCCTGGA+AGG - chr3_3:52904297-52904316 None:intergenic 45.0%
GAACTCTTATAGTTTCCCCC+TGG - chr3_3:52904301-52904320 None:intergenic 45.0%
CTGGAGGGATGAATCAACAG+AGG + chr3_3:52901584-52901603 Msa0457770:CDS 50.0%
! CGAAGCTTCTGAAGATCGGA+AGG - chr3_3:52902628-52902647 None:intergenic 50.0%
GCTGAGCCAATTGAGGAACT+CGG + chr3_3:52902908-52902927 Msa0457770:CDS 50.0%
GATCTCCCAGTCAAGAGTTC+TGG - chr3_3:52903082-52903101 None:intergenic 50.0%
!! TTATGTAGCACCGATGTGGG+TGG + chr3_3:52903254-52903273 Msa0457770:intron 50.0%
AAGGAAGATCTTGCCTTGCC+AGG + chr3_3:52903643-52903662 Msa0457770:CDS 50.0%
AAATATGGGCCGAGCGATTC+AGG + chr3_3:52903762-52903781 Msa0457770:CDS 50.0%
! CAGGGTCATAGGGCATTTAC+TGG + chr3_3:52903944-52903963 Msa0457770:intron 50.0%
!! GACATGTTTGGTGTCCGAGA+CGG + chr3_3:52904356-52904375 Msa0457770:three_prime_UTR 50.0%
AGTAGTGTCAATGCCGGACT+CGG - chr3_3:52904457-52904476 None:intergenic 50.0%
! ACGTGTCACTGTTGTGTGTC+TGG + chr3_3:52904494-52904513 Msa0457770:three_prime_UTR 50.0%
! TTGATGGGAGTACACCTGCA+GGG - chr3_3:52904573-52904592 None:intergenic 50.0%
! ATTGATGGGAGTACACCTGC+AGG - chr3_3:52904574-52904593 None:intergenic 50.0%
TGACAAGGCCAGTGGAAGGA+AGG + chr3_3:52902115-52902134 Msa0457770:CDS 55.0%
CAACTCGGAGTGGCATGAAG+TGG - chr3_3:52902144-52902163 None:intergenic 55.0%
CACTTCATGCCACTCCGAGT+TGG + chr3_3:52902142-52902161 Msa0457770:CDS 55.0%
CTCGTGATGACCACCCACAT+CGG - chr3_3:52903267-52903286 None:intergenic 55.0%
! TTGGGCTGGAGGGAAACCAT+TGG + chr3_3:52903567-52903586 Msa0457770:CDS 55.0%
! TGGGCTGGAGGGAAACCATT+GGG + chr3_3:52903568-52903587 Msa0457770:CDS 55.0%
! TGTCGGTGCTTCAGGGTCAT+AGG + chr3_3:52903933-52903952 Msa0457770:intron 55.0%
! GTCGGTGCTTCAGGGTCATA+GGG + chr3_3:52903934-52903953 Msa0457770:intron 55.0%
CTGGCTGCCCTAATGAGTCT+TGG - chr3_3:52904072-52904091 None:intergenic 55.0%
CATTAGGGCAGCCAGAGCTT+AGG + chr3_3:52904077-52904096 Msa0457770:CDS 55.0%
GCACTCAGGGACAGGATACT+TGG - chr3_3:52904128-52904147 None:intergenic 55.0%
TTGCACATGCACTCAGGGAC+AGG - chr3_3:52904136-52904155 None:intergenic 55.0%
!! TACCGGTGTCATGTCCGTCT+CGG - chr3_3:52904373-52904392 None:intergenic 55.0%
TCGTGTCTAGTGTCCGAGTC+CGG + chr3_3:52904441-52904460 Msa0457770:three_prime_UTR 55.0%
AGTGGAGGCCTTCCTTCCAC+TGG - chr3_3:52902126-52902145 None:intergenic 60.0%
CTCGGAGTGGCATGAAGTGG+AGG - chr3_3:52902141-52902160 None:intergenic 60.0%
TGCACGGATCCAACTCGGAG+TGG - chr3_3:52902154-52902173 None:intergenic 60.0%
GTGAGGACAGGTTGTCTCCC+TGG + chr3_3:52903007-52903026 Msa0457770:CDS 60.0%
GCTGGAGGGAAACCATTGGG+TGG + chr3_3:52903571-52903590 Msa0457770:CDS 60.0%
GTCCGAGACGGACATGACAC+CGG + chr3_3:52904368-52904387 Msa0457770:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 52900950 52904771 52900950 ID=Msa0457770;Name=Msa0457770
chr3_3 mRNA 52900950 52904771 52900950 ID=Msa0457770-mRNA-1;Parent=Msa0457770;Name=Msa0457770-mRNA-1;_AED=0.03;_eAED=0.03;_QI=60|1|1|1|1|1|6|594|573
chr3_3 exon 52900950 52901701 52900950 ID=Msa0457770-mRNA-1:exon:10233;Parent=Msa0457770-mRNA-1
chr3_3 exon 52902023 52902188 52902023 ID=Msa0457770-mRNA-1:exon:10234;Parent=Msa0457770-mRNA-1
chr3_3 exon 52902565 52902654 52902565 ID=Msa0457770-mRNA-1:exon:10235;Parent=Msa0457770-mRNA-1
chr3_3 exon 52902884 52903167 52902884 ID=Msa0457770-mRNA-1:exon:10236;Parent=Msa0457770-mRNA-1
chr3_3 exon 52903525 52903783 52903525 ID=Msa0457770-mRNA-1:exon:10237;Parent=Msa0457770-mRNA-1
chr3_3 exon 52903947 52904771 52903947 ID=Msa0457770-mRNA-1:exon:10238;Parent=Msa0457770-mRNA-1
chr3_3 five_prime_UTR 52900950 52901009 52900950 ID=Msa0457770-mRNA-1:five_prime_utr;Parent=Msa0457770-mRNA-1
chr3_3 CDS 52901010 52901701 52901010 ID=Msa0457770-mRNA-1:cds;Parent=Msa0457770-mRNA-1
chr3_3 CDS 52902023 52902188 52902023 ID=Msa0457770-mRNA-1:cds;Parent=Msa0457770-mRNA-1
chr3_3 CDS 52902565 52902654 52902565 ID=Msa0457770-mRNA-1:cds;Parent=Msa0457770-mRNA-1
chr3_3 CDS 52902884 52903167 52902884 ID=Msa0457770-mRNA-1:cds;Parent=Msa0457770-mRNA-1
chr3_3 CDS 52903525 52903783 52903525 ID=Msa0457770-mRNA-1:cds;Parent=Msa0457770-mRNA-1
chr3_3 CDS 52903947 52904177 52903947 ID=Msa0457770-mRNA-1:cds;Parent=Msa0457770-mRNA-1
chr3_3 three_prime_UTR 52904178 52904771 52904178 ID=Msa0457770-mRNA-1:three_prime_utr;Parent=Msa0457770-mRNA-1
Gene Sequence

>Msa0457770

ATGGCAAAATCCAAGAACAATGCAAAGAAGTTATCATACATATCAGTTCCATCACAAATCATAAACTCCATTTCATCTTCTTCACTTCAATCTCTCATTGATTCTCCAAAGAAGCCTTCAAGAACCACCATCTTCAACAGAAAATCAACACTATGGTTTCTTGCTCTGTTTCTTGTTGGTTTCTTTGGTATGTTCAAGTTTGGTTTCAACCCTGATACCTCATTTCCTCAAAAACCATGCTCCACAAATCTTCAGAAGTTCTCAATATCAAATCATCATTCATTTTCCAAACTTGGTTTTGGTAGTGTTCTCTTGCAGAAAGAGAGAAAAATATCTTTGAAAAATGACCATAATGACGAGTTTTTGCAAGAACAAGAACAAGAAAGAGGAGAGTTTTTGGTTTCTCATGTGCAATTTCAAGCAAATGGGTCTAGTGGATATGAGAAAAAGAGTGAGTTTTGGAAGAAACCAAATGGTTTAGGATATAAACCTTGTTTGAGTTTTAGTAATGATTATATGAGAGAAAGTGAAAGGGTTTTGAAGGATAGAAGAAAGTATCTTATGGTTGTTGTTTCTGGAGGGATGAATCAACAGAGGAATCAGATTGTTGATGCTGTTGTTATTGCTAGGATTCTTGGTGCTGCTTTGGTTGTTCCTGTTTTGCAAGTCAATGTTATTTGGGGTGATGAAAGTGAATTTGGTGACATATTTGATCTAGAACACTTCAAAAGAGTCCTTGCAAATGATGTGAGAGTGGTTTCAGCATTGCCATCAACACACATAATGACAAGGCCAGTGGAAGGAAGGCCTCCACTTCATGCCACTCCGAGTTGGATCCGTGCAAGATATCTCAGAAGATTCAATAGAGAAGGTGTTTTGCTTTTACGCGGCTTGGATTCAAGGCTTTCAAAGGATCTTCCTTCCGATCTTCAGAAGCTTCGTTGCAAGGTTGCTTTTAATGCATTAAGATTTGCTGAGCCAATTGAGGAACTCGGTAACAAGATCGCAGAGAGAATGAAAAGCAAAGGACCTTACCTTGCTCTTCATTTAAGAATGGAAAAAGATGTTTGGGTGAGGACAGGTTGTCTCCCTGGTCTAACTCCTGAGTATGATGAGGTTATTAACAATGAGAGAATACAAAGACCAGAACTCTTGACTGGGAGATCAAACATGACTTACCATCAAAGAAAAATGGCTGGACTTTGTCCTTTGAATGTTATCGAAGTTATGAGGTTACTGAAAGCTCTAGGAGCTCCAAAAGATGCAAAAATTTATTGGGCTGGAGGGAAACCATTGGGTGGAAAAGAAGCCTTACTTCCATTAATTCAAGAGTTTCCTAATTTTTACAATAAGGAAGATCTTGCCTTGCCAGGAGAATTACAACCATTTGTAAGGAAAGCTTCTCTAATGGCTGCCATAGATTACATAGTCTCTGAGAAGAGTGATGTTTTCATGCCATCTCATGGAGGAAATATGGGCCGAGCGATTCAGGGTCATAGGGCATTTACTGGACACAAGAAGTATATAACTCCAAACAAGAGACACATGCTTCCTTATTTTCTAAATTCTTCACTCTCTGAAGAAGAGTTCAATAGGATAATCAAAGAATTGCACCAAGACTCATTAGGGCAGCCAGAGCTTAGGACTAACAAAAATGGGAGAGATGTTACCAAGTATCCTGTCCCTGAGTGCATGTGCAATGATTCACATGCTCCATCCTGA
Protein sequence

>Msa0457770

MAKSKNNAKKLSYISVPSQIINSISSSSLQSLIDSPKKPSRTTIFNRKSTLWFLALFLVGFFGMFKFGFNPDTSFPQKPCSTNLQKFSISNHHSFSKLGFGSVLLQKERKISLKNDHNDEFLQEQEQERGEFLVSHVQFQANGSSGYEKKSEFWKKPNGLGYKPCLSFSNDYMRESERVLKDRRKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPVLQVNVIWGDESEFGDIFDLEHFKRVLANDVRVVSALPSTHIMTRPVEGRPPLHATPSWIRARYLRRFNREGVLLLRGLDSRLSKDLPSDLQKLRCKVAFNALRFAEPIEELGNKIAERMKSKGPYLALHLRMEKDVWVRTGCLPGLTPEYDEVINNERIQRPELLTGRSNMTYHQRKMAGLCPLNVIEVMRLLKALGAPKDAKIYWAGGKPLGGKEALLPLIQEFPNFYNKEDLALPGELQPFVRKASLMAAIDYIVSEKSDVFMPSHGGNMGRAIQGHRAFTGHKKYITPNKRHMLPYFLNSSLSEEEFNRIIKELHQDSLGQPELRTNKNGRDVTKYPVPECMCNDSHAPS*