AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0471020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0471020 MtrunA17_Chr3g0125811 86.567 201 27 0 18 218 4 204 3.33e-109 325
Msa0471020 MtrunA17_Chr1g0159461 46.305 203 70 5 20 189 1 197 2.50e-32 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 128 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTATGAGAATAAGAAATT+TGG 0.178504 3_3:-73231468 None:intergenic
GGTATTGTCCATAGTTAAAA+TGG 0.237581 3_3:+73233321 Msa0471020:three_prime_UTR
GGTACAACCATGTCATTAAT+TGG 0.248675 3_3:+73231361 Msa0471020:intron
CCATGTAGGCTGCAATTCTT+TGG 0.255636 3_3:-73232063 None:intergenic
GAAAAGGGTGCACTTCATTT+TGG 0.257256 3_3:+73233000 Msa0471020:three_prime_UTR
CACCAAATCTGATCCGAAAT+TGG 0.280682 3_3:-73231638 None:intergenic
GGTATGAAGTCGCGGGAAAA+TGG 0.287860 3_3:+73232866 Msa0471020:three_prime_UTR
GCTACTGATGCTGCTAATAT+TGG 0.307471 3_3:-73231896 None:intergenic
TCCACGACCGTTACAAGTTT+TGG 0.308347 3_3:-73232649 None:intergenic
AAGAAATAACGAAAATTTCT+AGG 0.316566 3_3:+73231926 Msa0471020:CDS
GATATGAATTATTATTATCT+TGG 0.320025 3_3:-73233064 None:intergenic
GTCCAATTTCGGATCAGATT+TGG 0.328151 3_3:+73231636 Msa0471020:CDS
TCGAAGCAAGTGTTTGAATC+AGG 0.332729 3_3:-73232321 None:intergenic
TTGATCCTAACATTATTGAT+TGG 0.340862 3_3:+73231797 Msa0471020:CDS
CTTATGAGAATAAGAAATTT+GGG 0.359390 3_3:-73231467 None:intergenic
TAACATAACCAGGAGGAAAA+TGG 0.364318 3_3:+73233228 Msa0471020:three_prime_UTR
TCAGCACCACGTTGCCTTGT+TGG 0.374411 3_3:-73231610 None:intergenic
GGTGCTGATCAGTCCAATTT+CGG 0.378562 3_3:+73231625 Msa0471020:CDS
TCACTCACATTTGTGCTCAT+TGG 0.383131 3_3:-73232925 None:intergenic
GTACAAGATAAAGGAGGAAA+AGG 0.383813 3_3:+73232984 Msa0471020:three_prime_UTR
GCCGCTCGCCTCGCTGAATC+CGG 0.387906 3_3:+73232097 Msa0471020:three_prime_UTR
ATTGAGGAAGAAGAGGAATA+TGG 0.391911 3_3:+73231754 Msa0471020:CDS
TGATCCTAACATTATTGATT+GGG 0.392056 3_3:+73231798 Msa0471020:CDS
AGATAATAATAATTCATATC+AGG 0.400968 3_3:+73233067 Msa0471020:three_prime_UTR
GGCAAGGGATATTGTTAATA+CGG 0.403210 3_3:+73232813 Msa0471020:three_prime_UTR
CATTCATAAAGAAGTTGCTT+GGG 0.414263 3_3:-73231951 None:intergenic
AACAGCAGCATATTCTGAAT+TGG 0.421315 3_3:-73231832 None:intergenic
CTGTGATAGGTACAAGATAA+AGG 0.425096 3_3:+73232975 Msa0471020:three_prime_UTR
GGGTTAACATCGATTTCCTT+TGG 0.428305 3_3:-73231433 None:intergenic
GTGAATTCAATTGAAGTTGA+TGG 0.432801 3_3:+73231676 Msa0471020:CDS
TCAGCCCAATCAATAATGTT+AGG 0.434921 3_3:-73231802 None:intergenic
ACCGTTGAAACGCTCTCGTC+AGG 0.440496 3_3:-73232583 None:intergenic
CCATCACATGTTAGATTGAC+CGG 0.440788 3_3:+73232355 Msa0471020:three_prime_UTR
ATGCTGCTAATATTGGAATC+TGG 0.446519 3_3:-73231889 None:intergenic
CATGTAGGCTGCAATTCTTT+GGG 0.448246 3_3:-73232062 None:intergenic
ATGCAAGGAACCTCCTTCTT+CGG 0.450058 3_3:+73232144 Msa0471020:three_prime_UTR
AAGTTAAGTTGTACTGTGAT+AGG 0.450787 3_3:+73232962 Msa0471020:three_prime_UTR
CCAAAGAATTGCAGCCTACA+TGG 0.451697 3_3:+73232063 Msa0471020:three_prime_UTR
GAGATCAAAACTAGAAGCAT+TGG 0.459866 3_3:+73231705 Msa0471020:CDS
TCTGGAGACGAATACTCCTT+TGG 0.473491 3_3:-73231871 None:intergenic
TTCGGATCAGATTTGGTGAT+GGG 0.474390 3_3:+73231643 Msa0471020:CDS
TGATTCAAACACTTGCTTCG+AGG 0.476019 3_3:+73232323 Msa0471020:three_prime_UTR
TACTCTTCCTAGAGAGAGCC+AGG 0.477900 3_3:+73232762 Msa0471020:three_prime_UTR
AGCATATTCTGAATTGGATC+AGG 0.480387 3_3:-73231826 None:intergenic
CGTCAGGCATGTGATGAAGC+TGG 0.484030 3_3:-73232567 None:intergenic
TTGCATGTGAAGGTGAAGAT+AGG 0.487421 3_3:+73232836 Msa0471020:three_prime_UTR
CACACTGCTTGATTCAGAGA+AGG 0.488605 3_3:-73230968 None:intergenic
TCTCAACATTCATCCTATCC+TGG 0.491515 3_3:-73232780 None:intergenic
GTTGGAGGACTAAAGATCAT+AGG 0.492091 3_3:+73232409 Msa0471020:three_prime_UTR
AGCGATGCAGATACTGTTCG+AGG 0.503528 3_3:+73232177 Msa0471020:three_prime_UTR
ACGAGACCAACTTCTTCGCT+TGG 0.503960 3_3:+73232612 Msa0471020:three_prime_UTR
ACATTCATAAAGAAGTTGCT+TGG 0.509000 3_3:-73231952 None:intergenic
ATCCTATCCTGGCTCTCTCT+AGG 0.512100 3_3:-73232769 None:intergenic
TCTTTACCAAGCGAAGAAGT+TGG 0.520115 3_3:-73232618 None:intergenic
TCGCGGGAAAATGGAGAGCT+AGG 0.521293 3_3:+73232875 Msa0471020:three_prime_UTR
GCTGCTAGACGGTCCGAAGA+AGG 0.532544 3_3:-73232157 None:intergenic
TCTGTGCAGCGATCCGTTGG+AGG 0.533052 3_3:+73232394 Msa0471020:three_prime_UTR
CGAAGCAAGTGTTTGAATCA+GGG 0.537450 3_3:-73232320 None:intergenic
GTAGAGATGTCAGAACTAGA+TGG 0.537559 3_3:-73231577 None:intergenic
GAGTCTGTGCAGCGATCCGT+TGG 0.538684 3_3:+73232391 Msa0471020:three_prime_UTR
TACCTTAAAACGCGATGTAG+CGG 0.540118 3_3:-73233302 None:intergenic
TCCAAAACTTGTAACGGTCG+TGG 0.541218 3_3:+73232648 Msa0471020:three_prime_UTR
TTTAAGTTGTTAACATAACC+AGG 0.541846 3_3:+73233218 Msa0471020:three_prime_UTR
TAAAATGGCCATCAGTGGGC+TGG 0.545429 3_3:+73233336 Msa0471020:three_prime_UTR
TTATAGAGCTTTGAAATGCA+AGG 0.546542 3_3:+73232129 Msa0471020:three_prime_UTR
TACTGTCGCCAGCCCACTGA+TGG 0.547247 3_3:-73233344 None:intergenic
TACAAGATAAAGGAGGAAAA+GGG 0.547340 3_3:+73232985 Msa0471020:three_prime_UTR
AGAGCTAGGATGACAATGGC+TGG 0.548519 3_3:+73232889 Msa0471020:three_prime_UTR
TTTGAATCCAAAACTTGTAA+CGG 0.552516 3_3:+73232642 Msa0471020:three_prime_UTR
AACGCGATGTAGCGGACATG+AGG 0.554890 3_3:-73233294 None:intergenic
TAGTGACATTGAGGAAGAAG+AGG 0.556415 3_3:+73231747 Msa0471020:CDS
ACGGATCGCTGCACAGACTC+AGG 0.557758 3_3:-73232388 None:intergenic
GAACAACTAGCAGAAACACT+TGG 0.558635 3_3:+73232442 Msa0471020:three_prime_UTR
ACTTGATGATAGTGACATTG+AGG 0.558721 3_3:+73231738 Msa0471020:CDS
CCTTTGGTGAGTCATGATCA+TGG 0.558992 3_3:-73231855 None:intergenic
TTTCGGATCAGATTTGGTGA+TGG 0.559466 3_3:+73231642 Msa0471020:CDS
ATATGGTAATTCTCAAAGCA+TGG 0.562265 3_3:+73231771 Msa0471020:CDS
AAATGATTGTTGCTTCAGCA+TGG 0.566279 3_3:+73233035 Msa0471020:three_prime_UTR
CCGGTCAATCTAACATGTGA+TGG 0.566500 3_3:-73232355 None:intergenic
TAGTTAAAATGGCCATCAGT+GGG 0.567643 3_3:+73233332 Msa0471020:three_prime_UTR
CATCACATGTTAGATTGACC+GGG 0.567991 3_3:+73232356 Msa0471020:three_prime_UTR
CTCGTAACTCTCGCTCGAGT+AGG 0.572630 3_3:-73231515 None:intergenic
TTGAGAGGCAATGCTTGGCA+AGG 0.577561 3_3:+73232797 Msa0471020:three_prime_UTR
AATTGCAGCCTACATGGTGG+AGG 0.577669 3_3:+73232069 Msa0471020:three_prime_UTR
TAGGCTGCAATTCTTTGGGA+AGG 0.583102 3_3:-73232058 None:intergenic
CAAAACTAGAAGCATTGGAG+AGG 0.583413 3_3:+73231710 Msa0471020:CDS
GAATGTTGAGAGGCAATGCT+TGG 0.583862 3_3:+73232792 Msa0471020:three_prime_UTR
CTACAAGTTTCATGCCAACA+AGG 0.584293 3_3:+73231596 Msa0471020:CDS
TGTGCTACTGCAATTTCAGA+AGG 0.584377 3_3:+73231972 Msa0471020:CDS
GCCTGACGAGAGCGTTTCAA+CGG 0.590858 3_3:+73232582 Msa0471020:three_prime_UTR
TCCGGATTCAGCGAGGCGAG+CGG 0.592435 3_3:-73232098 None:intergenic
CACTAAGCTCAAATCGCCAA+AGG 0.608012 3_3:+73231417 Msa0471020:CDS
TATGATCTTTAGTCCTCCAA+CGG 0.609253 3_3:-73232407 None:intergenic
GTATCTGCATCGCTGCTAGA+CGG 0.609923 3_3:-73232168 None:intergenic
GTTAATACGGTTGCATGTGA+AGG 0.612068 3_3:+73232826 Msa0471020:three_prime_UTR
ATAGAACGGTATGAAGTCGC+GGG 0.612894 3_3:+73232859 Msa0471020:three_prime_UTR
AGCATGAAACAGCTATGCTT+AGG 0.613918 3_3:+73233251 Msa0471020:three_prime_UTR
AGACTCAGGATCATCAATCC+CGG 0.614792 3_3:-73232374 None:intergenic
TAACTATGCAATAACAGAAG+CGG 0.616342 3_3:+73232234 Msa0471020:three_prime_UTR
GATAGAACGGTATGAAGTCG+CGG 0.616637 3_3:+73232858 Msa0471020:three_prime_UTR
TCGTCGTGGAGAAGCTCTTG+TGG 0.619304 3_3:+73232531 Msa0471020:three_prime_UTR
AAGGTGAAGATAGGATAGAA+CGG 0.625100 3_3:+73232845 Msa0471020:three_prime_UTR
TTCCTAGAGAGAGCCAGGAT+AGG 0.625909 3_3:+73232767 Msa0471020:three_prime_UTR
CTTGAATTGAGACAATTCGT+CGG 0.634697 3_3:-73232031 None:intergenic
TGAGAGGCAATGCTTGGCAA+GGG 0.635494 3_3:+73232798 Msa0471020:three_prime_UTR
TCGATGCTTGATTGTCGTCG+TGG 0.642078 3_3:+73232517 Msa0471020:three_prime_UTR
ATAGTTAAAATGGCCATCAG+TGG 0.643912 3_3:+73233331 Msa0471020:three_prime_UTR
AAAACTAGAAGCATTGGAGA+GGG 0.649054 3_3:+73231711 Msa0471020:CDS
AGAAGATCCAATTAATGACA+TGG 0.650375 3_3:-73231368 None:intergenic
ATTGCAGCCTACATGGTGGA+GGG 0.650477 3_3:+73232070 Msa0471020:three_prime_UTR
GGCGAGTCCCTCCACCATGT+AGG 0.652264 3_3:-73232077 None:intergenic
GCTAGACGGTCCGAAGAAGG+AGG 0.655079 3_3:-73232154 None:intergenic
ATTCATAAAGAAGTTGCTTG+GGG 0.655928 3_3:-73231950 None:intergenic
CCATGATCATGACTCACCAA+AGG 0.657257 3_3:+73231855 Msa0471020:CDS
ATGAGAATAAGAAATTTGGG+AGG 0.658872 3_3:-73231464 None:intergenic
AGGATAGGATGAATGTTGAG+AGG 0.660140 3_3:+73232782 Msa0471020:three_prime_UTR
AATTTCAGAAGGAAATGAAG+AGG 0.662867 3_3:+73231983 Msa0471020:CDS
ATGGAGAGCTAGGATGACAA+TGG 0.667953 3_3:+73232885 Msa0471020:three_prime_UTR
GTGTACAGAACTATTAAACA+AGG 0.668012 3_3:-73233137 None:intergenic
AATTTCGAGCGGTCTCATCG+AGG 0.679065 3_3:+73232476 Msa0471020:three_prime_UTR
TTTGTCATTTGAATTTCGAG+CGG 0.680630 3_3:+73232465 Msa0471020:three_prime_UTR
AAGAATTGCAGCCTACATGG+TGG 0.682524 3_3:+73232066 Msa0471020:three_prime_UTR
TGATAGGTACAAGATAAAGG+AGG 0.692576 3_3:+73232978 Msa0471020:three_prime_UTR
ACGACAATCAAGCATCGATG+AGG 0.692866 3_3:-73232512 None:intergenic
ATAAACCCGAATTTAAAACA+CGG 0.716869 3_3:-73232205 None:intergenic
TTCATGCCAACAAGGCAACG+TGG 0.722675 3_3:+73231604 Msa0471020:CDS
AAGTTGTTAACATAACCAGG+AGG 0.731266 3_3:+73233221 Msa0471020:three_prime_UTR
TGATTGTTGCTTCAGCATGG+AGG 0.760816 3_3:+73233038 Msa0471020:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAGAAAAAAAATTAAAATT+TGG - chr3_3:73230498-73230517 None:intergenic 10.0%
!! AATAAGAAGAAGAATAAATT+TGG + chr3_3:73230669-73230688 Msa0471020:intron 15.0%
!! ATCATTACAAAATATTATAG+TGG - chr3_3:73230806-73230825 None:intergenic 15.0%
!!! AATATTTTGTAATGATAAAG+TGG + chr3_3:73230810-73230829 Msa0471020:intron 15.0%
!! GATATGAATTATTATTATCT+TGG - chr3_3:73233067-73233086 None:intergenic 15.0%
!! AGATAATAATAATTCATATC+AGG + chr3_3:73233067-73233086 Msa0471020:three_prime_UTR 15.0%
!!! TCAATGTTGATTTATTTGTA+AGG + chr3_3:73230780-73230799 Msa0471020:intron 20.0%
!! ATGAATATGACAAAATATAC+TGG - chr3_3:73231133-73231152 None:intergenic 20.0%
!! CTTATGAGAATAAGAAATTT+GGG - chr3_3:73231470-73231489 None:intergenic 20.0%
!!! AGAAATTTTCGTTATTTCTT+TGG - chr3_3:73231926-73231945 None:intergenic 20.0%
!! AAGAAATAACGAAAATTTCT+AGG + chr3_3:73231926-73231945 Msa0471020:CDS 20.0%
!!! ATAGATTTTGATATCAATCA+AGG + chr3_3:73232283-73232302 Msa0471020:three_prime_UTR 20.0%
!! TAAATTTGGTTTCTTCATAG+TGG + chr3_3:73230683-73230702 Msa0471020:intron 25.0%
!!! TAGTCAAACATAAGTTGATT+TGG - chr3_3:73230847-73230866 None:intergenic 25.0%
!! AATCAGCTTTTGTTAGAAAA+AGG + chr3_3:73230875-73230894 Msa0471020:intron 25.0%
! TGTGAAAATCATTATTCTCT+TGG + chr3_3:73230898-73230917 Msa0471020:intron 25.0%
! ACAATTACTATGATATCACT+TGG - chr3_3:73230928-73230947 None:intergenic 25.0%
!! TCATAGAATCTTTTCAGAAT+TGG + chr3_3:73231021-73231040 Msa0471020:intron 25.0%
!! AAACTTAGAAAATCCTTTTC+AGG - chr3_3:73231160-73231179 None:intergenic 25.0%
! GCTTATGAGAATAAGAAATT+TGG - chr3_3:73231471-73231490 None:intergenic 25.0%
! TTGATCCTAACATTATTGAT+TGG + chr3_3:73231797-73231816 Msa0471020:CDS 25.0%
!! TGATCCTAACATTATTGATT+GGG + chr3_3:73231798-73231817 Msa0471020:CDS 25.0%
!!! AATTTTCGTTATTTCTTTGG+TGG - chr3_3:73231923-73231942 None:intergenic 25.0%
!!! AGCTCTATAAATACTTTTTC+CGG - chr3_3:73232119-73232138 None:intergenic 25.0%
!! ATAAACCCGAATTTAAAACA+CGG - chr3_3:73232208-73232227 None:intergenic 25.0%
! TTTGAATCCAAAACTTGTAA+CGG + chr3_3:73232642-73232661 Msa0471020:three_prime_UTR 25.0%
!!! AGTACAACTTAACTTGTTTT+CGG - chr3_3:73232957-73232976 None:intergenic 25.0%
!!! TTTAAGTTGTTAACATAACC+AGG + chr3_3:73233218-73233237 Msa0471020:three_prime_UTR 25.0%
AAAGTGATAAAACCAGGAAA+AGG - chr3_3:73230554-73230573 None:intergenic 30.0%
TAGTTCAAAGTGATAAAACC+AGG - chr3_3:73230560-73230579 None:intergenic 30.0%
!! TATGTACTCATCTTTTGAGT+TGG + chr3_3:73230578-73230597 Msa0471020:intron 30.0%
TTGGAATTTCAGACAAGAAT+AGG + chr3_3:73230597-73230616 Msa0471020:intron 30.0%
!!! AAATTTTTTCCCTTGTTAGC+TGG - chr3_3:73230728-73230747 None:intergenic 30.0%
TGTATAGTTTCAATCTTAGC+AGG + chr3_3:73230945-73230964 Msa0471020:intron 30.0%
! AATCTTTTCAGAATTGGAGT+TGG + chr3_3:73231027-73231046 Msa0471020:intron 30.0%
CATATTCATCATACCTGAAA+AGG + chr3_3:73231144-73231163 Msa0471020:intron 30.0%
GTGAGAAAGCAAAAACTTTA+TGG + chr3_3:73231280-73231299 Msa0471020:intron 30.0%
AGAAGATCCAATTAATGACA+TGG - chr3_3:73231371-73231390 None:intergenic 30.0%
AAATTTGGGAGGAAAAATCA+GGG - chr3_3:73231456-73231475 None:intergenic 30.0%
ATGAGAATAAGAAATTTGGG+AGG - chr3_3:73231467-73231486 None:intergenic 30.0%
GTGAATTCAATTGAAGTTGA+TGG + chr3_3:73231676-73231695 Msa0471020:CDS 30.0%
ATATGGTAATTCTCAAAGCA+TGG + chr3_3:73231771-73231790 Msa0471020:CDS 30.0%
ATTCATAAAGAAGTTGCTTG+GGG - chr3_3:73231953-73231972 None:intergenic 30.0%
CATTCATAAAGAAGTTGCTT+GGG - chr3_3:73231954-73231973 None:intergenic 30.0%
ACATTCATAAAGAAGTTGCT+TGG - chr3_3:73231955-73231974 None:intergenic 30.0%
AATTTCAGAAGGAAATGAAG+AGG + chr3_3:73231983-73232002 Msa0471020:CDS 30.0%
!! TTATAGAGCTTTGAAATGCA+AGG + chr3_3:73232129-73232148 Msa0471020:three_prime_UTR 30.0%
!! GTTTGTCCGTGTTTTAAATT+CGG + chr3_3:73232199-73232218 Msa0471020:three_prime_UTR 30.0%
!! TTTGTCCGTGTTTTAAATTC+GGG + chr3_3:73232200-73232219 Msa0471020:three_prime_UTR 30.0%
TAACTATGCAATAACAGAAG+CGG + chr3_3:73232234-73232253 Msa0471020:three_prime_UTR 30.0%
! TTTGTCATTTGAATTTCGAG+CGG + chr3_3:73232465-73232484 Msa0471020:three_prime_UTR 30.0%
TCAACAAATCTCTGCAAAAA+CGG - chr3_3:73232700-73232719 None:intergenic 30.0%
! AAGTTAAGTTGTACTGTGAT+AGG + chr3_3:73232962-73232981 Msa0471020:three_prime_UTR 30.0%
TACAAGATAAAGGAGGAAAA+GGG + chr3_3:73232985-73233004 Msa0471020:three_prime_UTR 30.0%
GTGTACAGAACTATTAAACA+AGG - chr3_3:73233140-73233159 None:intergenic 30.0%
GGTATTGTCCATAGTTAAAA+TGG + chr3_3:73233321-73233340 Msa0471020:three_prime_UTR 30.0%
TATATGTGTCTGCTTCCAAA+GGG + chr3_3:73230518-73230537 Msa0471020:intron 35.0%
ACAAGAATAGGTTCAAAGCA+GGG + chr3_3:73230609-73230628 Msa0471020:intron 35.0%
GATATTCTTCCAGCTAACAA+GGG + chr3_3:73230716-73230735 Msa0471020:intron 35.0%
AGAATTGGAGTTGGACATAA+TGG + chr3_3:73231036-73231055 Msa0471020:intron 35.0%
TCAAATCATCATGAATCAGC+TGG - chr3_3:73231070-73231089 None:intergenic 35.0%
!! TTCTTTTGTTTCTCCCATCA+TGG + chr3_3:73231186-73231205 Msa0471020:intron 35.0%
TTATGTGTGAACTGAATGAC+AGG + chr3_3:73231340-73231359 Msa0471020:intron 35.0%
GGTACAACCATGTCATTAAT+TGG + chr3_3:73231361-73231380 Msa0471020:intron 35.0%
! TTAGTGCTTTTATGTGATGG+TGG - chr3_3:73231403-73231422 None:intergenic 35.0%
! AGCTTAGTGCTTTTATGTGA+TGG - chr3_3:73231406-73231425 None:intergenic 35.0%
GAAATTTGGGAGGAAAAATC+AGG - chr3_3:73231457-73231476 None:intergenic 35.0%
! GAGATCAAAACTAGAAGCAT+TGG + chr3_3:73231705-73231724 Msa0471020:CDS 35.0%
!! AAAACTAGAAGCATTGGAGA+GGG + chr3_3:73231711-73231730 Msa0471020:CDS 35.0%
! ACTTGATGATAGTGACATTG+AGG + chr3_3:73231738-73231757 Msa0471020:CDS 35.0%
ATTGAGGAAGAAGAGGAATA+TGG + chr3_3:73231754-73231773 Msa0471020:CDS 35.0%
TCAGCCCAATCAATAATGTT+AGG - chr3_3:73231805-73231824 None:intergenic 35.0%
AGCATATTCTGAATTGGATC+AGG - chr3_3:73231829-73231848 None:intergenic 35.0%
AACAGCAGCATATTCTGAAT+TGG - chr3_3:73231835-73231854 None:intergenic 35.0%
ATGCTGCTAATATTGGAATC+TGG - chr3_3:73231892-73231911 None:intergenic 35.0%
CTTGAATTGAGACAATTCGT+CGG - chr3_3:73232034-73232053 None:intergenic 35.0%
TATGATCTTTAGTCCTCCAA+CGG - chr3_3:73232410-73232429 None:intergenic 35.0%
AACAAATCTCTGCAAAAACG+GGG - chr3_3:73232698-73232717 None:intergenic 35.0%
CAACAAATCTCTGCAAAAAC+GGG - chr3_3:73232699-73232718 None:intergenic 35.0%
GGCAAGGGATATTGTTAATA+CGG + chr3_3:73232813-73232832 Msa0471020:three_prime_UTR 35.0%
AAGGTGAAGATAGGATAGAA+CGG + chr3_3:73232845-73232864 Msa0471020:three_prime_UTR 35.0%
CTGTGATAGGTACAAGATAA+AGG + chr3_3:73232975-73232994 Msa0471020:three_prime_UTR 35.0%
TGATAGGTACAAGATAAAGG+AGG + chr3_3:73232978-73232997 Msa0471020:three_prime_UTR 35.0%
GTACAAGATAAAGGAGGAAA+AGG + chr3_3:73232984-73233003 Msa0471020:three_prime_UTR 35.0%
! AAATGATTGTTGCTTCAGCA+TGG + chr3_3:73233035-73233054 Msa0471020:three_prime_UTR 35.0%
AAGTTGTTAACATAACCAGG+AGG + chr3_3:73233221-73233240 Msa0471020:three_prime_UTR 35.0%
TAACATAACCAGGAGGAAAA+TGG + chr3_3:73233228-73233247 Msa0471020:three_prime_UTR 35.0%
!!! ACTGATGGCCATTTTAACTA+TGG - chr3_3:73233332-73233351 None:intergenic 35.0%
ATAGTTAAAATGGCCATCAG+TGG + chr3_3:73233331-73233350 Msa0471020:three_prime_UTR 35.0%
TAGTTAAAATGGCCATCAGT+GGG + chr3_3:73233332-73233351 Msa0471020:three_prime_UTR 35.0%
GTATATGTGTCTGCTTCCAA+AGG + chr3_3:73230517-73230536 Msa0471020:intron 40.0%
GACAAGAATAGGTTCAAAGC+AGG + chr3_3:73230608-73230627 Msa0471020:intron 40.0%
TGGTTTCTTCATAGTGGTTG+TGG + chr3_3:73230689-73230708 Msa0471020:intron 40.0%
GGATATTCTTCCAGCTAACA+AGG + chr3_3:73230715-73230734 Msa0471020:intron 40.0%
TCTTGTTGACCGTATTCTCA+AGG + chr3_3:73231258-73231277 Msa0471020:intron 40.0%
GCTTTCTCACCTTGAGAATA+CGG - chr3_3:73231270-73231289 None:intergenic 40.0%
!! GGGTTAACATCGATTTCCTT+TGG - chr3_3:73231436-73231455 None:intergenic 40.0%
GTAGAGATGTCAGAACTAGA+TGG - chr3_3:73231580-73231599 None:intergenic 40.0%
CTACAAGTTTCATGCCAACA+AGG + chr3_3:73231596-73231615 Msa0471020:CDS 40.0%
GTCCAATTTCGGATCAGATT+TGG + chr3_3:73231636-73231655 Msa0471020:CDS 40.0%
CACCAAATCTGATCCGAAAT+TGG - chr3_3:73231641-73231660 None:intergenic 40.0%
!! TTTCGGATCAGATTTGGTGA+TGG + chr3_3:73231642-73231661 Msa0471020:CDS 40.0%
!! TTCGGATCAGATTTGGTGAT+GGG + chr3_3:73231643-73231662 Msa0471020:CDS 40.0%
!! CAAAACTAGAAGCATTGGAG+AGG + chr3_3:73231710-73231729 Msa0471020:CDS 40.0%
TAGTGACATTGAGGAAGAAG+AGG + chr3_3:73231747-73231766 Msa0471020:CDS 40.0%
GCTACTGATGCTGCTAATAT+TGG - chr3_3:73231899-73231918 None:intergenic 40.0%
! TGTGCTACTGCAATTTCAGA+AGG + chr3_3:73231972-73231991 Msa0471020:CDS 40.0%
CATGTAGGCTGCAATTCTTT+GGG - chr3_3:73232065-73232084 None:intergenic 40.0%
CGAAGCAAGTGTTTGAATCA+GGG - chr3_3:73232323-73232342 None:intergenic 40.0%
TCGAAGCAAGTGTTTGAATC+AGG - chr3_3:73232324-73232343 None:intergenic 40.0%
TGATTCAAACACTTGCTTCG+AGG + chr3_3:73232323-73232342 Msa0471020:three_prime_UTR 40.0%
CCATCACATGTTAGATTGAC+CGG + chr3_3:73232355-73232374 Msa0471020:three_prime_UTR 40.0%
CATCACATGTTAGATTGACC+GGG + chr3_3:73232356-73232375 Msa0471020:three_prime_UTR 40.0%
GTTGGAGGACTAAAGATCAT+AGG + chr3_3:73232409-73232428 Msa0471020:three_prime_UTR 40.0%
GAACAACTAGCAGAAACACT+TGG + chr3_3:73232442-73232461 Msa0471020:three_prime_UTR 40.0%
TCTTTACCAAGCGAAGAAGT+TGG - chr3_3:73232621-73232640 None:intergenic 40.0%
TCTCAACATTCATCCTATCC+TGG - chr3_3:73232783-73232802 None:intergenic 40.0%
AGGATAGGATGAATGTTGAG+AGG + chr3_3:73232782-73232801 Msa0471020:three_prime_UTR 40.0%
GTTAATACGGTTGCATGTGA+AGG + chr3_3:73232826-73232845 Msa0471020:three_prime_UTR 40.0%
TTGCATGTGAAGGTGAAGAT+AGG + chr3_3:73232836-73232855 Msa0471020:three_prime_UTR 40.0%
TCACTCACATTTGTGCTCAT+TGG - chr3_3:73232928-73232947 None:intergenic 40.0%
!! GAAAAGGGTGCACTTCATTT+TGG + chr3_3:73233000-73233019 Msa0471020:three_prime_UTR 40.0%
AGCATGAAACAGCTATGCTT+AGG + chr3_3:73233251-73233270 Msa0471020:three_prime_UTR 40.0%
TACCTTAAAACGCGATGTAG+CGG - chr3_3:73233305-73233324 None:intergenic 40.0%
AAAGGCTACACAACACCCTT+TGG - chr3_3:73230536-73230555 None:intergenic 45.0%
AGAATAGGTTCAAAGCAGGG+AGG + chr3_3:73230612-73230631 Msa0471020:intron 45.0%
TCTTCATAGTGGTTGTGGCT+AGG + chr3_3:73230694-73230713 Msa0471020:intron 45.0%
CACACTGCTTGATTCAGAGA+AGG - chr3_3:73230971-73230990 None:intergenic 45.0%
TAGCATGACTGTGCCATGAT+GGG - chr3_3:73231202-73231221 None:intergenic 45.0%
CACTAAGCTCAAATCGCCAA+AGG + chr3_3:73231417-73231436 Msa0471020:CDS 45.0%
!! GGTGCTGATCAGTCCAATTT+CGG + chr3_3:73231625-73231644 Msa0471020:CDS 45.0%
CCTTTGGTGAGTCATGATCA+TGG - chr3_3:73231858-73231877 None:intergenic 45.0%
CCATGATCATGACTCACCAA+AGG + chr3_3:73231855-73231874 Msa0471020:CDS 45.0%
TCTGGAGACGAATACTCCTT+TGG - chr3_3:73231874-73231893 None:intergenic 45.0%
! TAGGCTGCAATTCTTTGGGA+AGG - chr3_3:73232061-73232080 None:intergenic 45.0%
CCATGTAGGCTGCAATTCTT+TGG - chr3_3:73232066-73232085 None:intergenic 45.0%
CCAAAGAATTGCAGCCTACA+TGG + chr3_3:73232063-73232082 Msa0471020:three_prime_UTR 45.0%
AAGAATTGCAGCCTACATGG+TGG + chr3_3:73232066-73232085 Msa0471020:three_prime_UTR 45.0%
!!! TACTTTTTCCGGATTCAGCG+AGG - chr3_3:73232108-73232127 None:intergenic 45.0%
ATGCAAGGAACCTCCTTCTT+CGG + chr3_3:73232144-73232163 Msa0471020:three_prime_UTR 45.0%
CCGGTCAATCTAACATGTGA+TGG - chr3_3:73232358-73232377 None:intergenic 45.0%
AGACTCAGGATCATCAATCC+CGG - chr3_3:73232377-73232396 None:intergenic 45.0%
!! ACGACAATCAAGCATCGATG+AGG - chr3_3:73232515-73232534 None:intergenic 45.0%
TCCAAAACTTGTAACGGTCG+TGG + chr3_3:73232648-73232667 Msa0471020:three_prime_UTR 45.0%
! TCCACGACCGTTACAAGTTT+TGG - chr3_3:73232652-73232671 None:intergenic 45.0%
AAATCTCTGCAAAAACGGGG+CGG - chr3_3:73232695-73232714 None:intergenic 45.0%
GAATGTTGAGAGGCAATGCT+TGG + chr3_3:73232792-73232811 Msa0471020:three_prime_UTR 45.0%
GATAGAACGGTATGAAGTCG+CGG + chr3_3:73232858-73232877 Msa0471020:three_prime_UTR 45.0%
ATAGAACGGTATGAAGTCGC+GGG + chr3_3:73232859-73232878 Msa0471020:three_prime_UTR 45.0%
ATGGAGAGCTAGGATGACAA+TGG + chr3_3:73232885-73232904 Msa0471020:three_prime_UTR 45.0%
!! AGGGTGCACTTCATTTTGGT+TGG + chr3_3:73233004-73233023 Msa0471020:three_prime_UTR 45.0%
!!! GGGTGCACTTCATTTTGGTT+GGG + chr3_3:73233005-73233024 Msa0471020:three_prime_UTR 45.0%
! TGATTGTTGCTTCAGCATGG+AGG + chr3_3:73233038-73233057 Msa0471020:three_prime_UTR 45.0%
! TTCATGCTCCATTTTCCTCC+TGG - chr3_3:73233239-73233258 None:intergenic 45.0%
!! GGTGTTGTGTAGCCTTTTCC+TGG + chr3_3:73230539-73230558 Msa0471020:intron 50.0%
CTAGCATGACTGTGCCATGA+TGG - chr3_3:73231203-73231222 None:intergenic 50.0%
TTCATGCCAACAAGGCAACG+TGG + chr3_3:73231604-73231623 Msa0471020:CDS 50.0%
AATTGCAGCCTACATGGTGG+AGG + chr3_3:73232069-73232088 Msa0471020:three_prime_UTR 50.0%
! ATTGCAGCCTACATGGTGGA+GGG + chr3_3:73232070-73232089 Msa0471020:three_prime_UTR 50.0%
GTATCTGCATCGCTGCTAGA+CGG - chr3_3:73232171-73232190 None:intergenic 50.0%
AGCGATGCAGATACTGTTCG+AGG + chr3_3:73232177-73232196 Msa0471020:three_prime_UTR 50.0%
AATTTCGAGCGGTCTCATCG+AGG + chr3_3:73232476-73232495 Msa0471020:three_prime_UTR 50.0%
TCGATGCTTGATTGTCGTCG+TGG + chr3_3:73232517-73232536 Msa0471020:three_prime_UTR 50.0%
ACGAGACCAACTTCTTCGCT+TGG + chr3_3:73232612-73232631 Msa0471020:three_prime_UTR 50.0%
TACTCTTCCTAGAGAGAGCC+AGG + chr3_3:73232762-73232781 Msa0471020:three_prime_UTR 50.0%
TTCCTAGAGAGAGCCAGGAT+AGG + chr3_3:73232767-73232786 Msa0471020:three_prime_UTR 50.0%
! ATCCTATCCTGGCTCTCTCT+AGG - chr3_3:73232772-73232791 None:intergenic 50.0%
TTGAGAGGCAATGCTTGGCA+AGG + chr3_3:73232797-73232816 Msa0471020:three_prime_UTR 50.0%
TGAGAGGCAATGCTTGGCAA+GGG + chr3_3:73232798-73232817 Msa0471020:three_prime_UTR 50.0%
GGTATGAAGTCGCGGGAAAA+TGG + chr3_3:73232866-73232885 Msa0471020:three_prime_UTR 50.0%
AGAGCTAGGATGACAATGGC+TGG + chr3_3:73232889-73232908 Msa0471020:three_prime_UTR 50.0%
! GTCCGCTACATCGCGTTTTA+AGG + chr3_3:73233300-73233319 Msa0471020:three_prime_UTR 50.0%
TAAAATGGCCATCAGTGGGC+TGG + chr3_3:73233336-73233355 Msa0471020:three_prime_UTR 50.0%
CTCGTAACTCTCGCTCGAGT+AGG - chr3_3:73231518-73231537 None:intergenic 55.0%
TCAGCACCACGTTGCCTTGT+TGG - chr3_3:73231613-73231632 None:intergenic 55.0%
TCGTCGTGGAGAAGCTCTTG+TGG + chr3_3:73232531-73232550 Msa0471020:three_prime_UTR 55.0%
CGTCAGGCATGTGATGAAGC+TGG - chr3_3:73232570-73232589 None:intergenic 55.0%
!! GCCTGACGAGAGCGTTTCAA+CGG + chr3_3:73232582-73232601 Msa0471020:three_prime_UTR 55.0%
ACCGTTGAAACGCTCTCGTC+AGG - chr3_3:73232586-73232605 None:intergenic 55.0%
CTGCAAAAACGGGGCGGTAT+TGG - chr3_3:73232689-73232708 None:intergenic 55.0%
TCGCGGGAAAATGGAGAGCT+AGG + chr3_3:73232875-73232894 Msa0471020:three_prime_UTR 55.0%
!! AACGCGATGTAGCGGACATG+AGG - chr3_3:73233297-73233316 None:intergenic 55.0%
GCTAGACGGTCCGAAGAAGG+AGG - chr3_3:73232157-73232176 None:intergenic 60.0%
GCTGCTAGACGGTCCGAAGA+AGG - chr3_3:73232160-73232179 None:intergenic 60.0%
ACGGATCGCTGCACAGACTC+AGG - chr3_3:73232391-73232410 None:intergenic 60.0%
GAGTCTGTGCAGCGATCCGT+TGG + chr3_3:73232391-73232410 Msa0471020:three_prime_UTR 60.0%
TCTGTGCAGCGATCCGTTGG+AGG + chr3_3:73232394-73232413 Msa0471020:three_prime_UTR 60.0%
TACTGTCGCCAGCCCACTGA+TGG - chr3_3:73233347-73233366 None:intergenic 60.0%
GGCGAGTCCCTCCACCATGT+AGG - chr3_3:73232080-73232099 None:intergenic 65.0%
TCCGGATTCAGCGAGGCGAG+CGG - chr3_3:73232101-73232120 None:intergenic 65.0%
GCCGCTCGCCTCGCTGAATC+CGG + chr3_3:73232097-73232116 Msa0471020:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 73230474 73233375 73230474 ID=Msa0471020;Name=Msa0471020
chr3_3 mRNA 73230474 73233375 73230474 ID=Msa0471020-mRNA-1;Parent=Msa0471020;Name=Msa0471020-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0.25|0.25|1|1|4|1355|235
chr3_3 exon 73230474 73230479 73230474 ID=Msa0471020-mRNA-1:exon:18523;Parent=Msa0471020-mRNA-1
chr3_3 exon 73230891 73230896 73230891 ID=Msa0471020-mRNA-1:exon:18524;Parent=Msa0471020-mRNA-1
chr3_3 exon 73230967 73231003 73230967 ID=Msa0471020-mRNA-1:exon:18525;Parent=Msa0471020-mRNA-1
chr3_3 exon 73231362 73233375 73231362 ID=Msa0471020-mRNA-1:exon:18526;Parent=Msa0471020-mRNA-1
chr3_3 CDS 73230474 73230479 73230474 ID=Msa0471020-mRNA-1:cds;Parent=Msa0471020-mRNA-1
chr3_3 CDS 73230891 73230896 73230891 ID=Msa0471020-mRNA-1:cds;Parent=Msa0471020-mRNA-1
chr3_3 CDS 73230967 73231003 73230967 ID=Msa0471020-mRNA-1:cds;Parent=Msa0471020-mRNA-1
chr3_3 CDS 73231362 73232020 73231362 ID=Msa0471020-mRNA-1:cds;Parent=Msa0471020-mRNA-1
chr3_3 three_prime_UTR 73232021 73233375 73232021 ID=Msa0471020-mRNA-1:three_prime_utr;Parent=Msa0471020-mRNA-1
Gene Sequence

>Msa0471020

ATGCATAAAAAGGCCTTCTCTGAATCAAGCAGTGTGAAAGTTTTTGCTAGTACAACCATGTCATTAATTGGATCTTCTGAGCTAGCTCCACCATCACATAAAAGCACTAAGCTCAAATCGCCAAAGGAAATCGATGTTAACCCTGATTTTTCCTCCCAAATTTCTTATTCTCATAAGCATAGAAGCATGTATATCACAGATACCTACTCGAGCGAGAGTTACGAGAAGTACTTCAATGACTCACAGACAGAAGAACTAATAGAACCATCTAGTTCTGACATCTCTACAAGTTTCATGCCAACAAGGCAACGTGGTGCTGATCAGTCCAATTTCGGATCAGATTTGGTGATGGGAAATCGAAGCGTGAATTCAATTGAAGTTGATGGTGCAATGAGATCAAAACTAGAAGCATTGGAGAGGGCATTACTTGATGATAGTGACATTGAGGAAGAAGAGGAATATGGTAATTCTCAAAGCATGGAAATTGATCCTAACATTATTGATTGGGCTGATCCTGATCCAATTCAGAATATGCTGCTGTTCCATGATCATGACTCACCAAAGGAGTATTCGTCTCCAGATTCCAATATTAGCAGCATCAGTAGCACCACCAAAGAAATAACGAAAATTTCTAGGACCCCAAGCAACTTCTTTATGAATGTGCTACTGCAATTTCAGAAGGAAATGAAGAGGAAGCATCGTCAATGA
Protein sequence

>Msa0471020

MHKKAFSESSSVKVFASTTMSLIGSSELAPPSHKSTKLKSPKEIDVNPDFSSQISYSHKHRSMYITDTYSSESYEKYFNDSQTEELIEPSSSDISTSFMPTRQRGADQSNFGSDLVMGNRSVNSIEVDGAMRSKLEALERALLDDSDIEEEEEYGNSQSMEIDPNIIDWADPDPIQNMLLFHDHDSPKEYSSPDSNISSISSTTKEITKISRTPSNFFMNVLLQFQKEMKRKHRQ*