AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0515820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515820 MtrunA17_Chr3g0125811 94.690 565 30 0 1 565 6 570 0.0 1080
Msa0515820 MtrunA17_Chr1g0159461 69.950 599 139 7 1 565 1 592 0.0 794
Msa0515820 MtrunA17_Chr5g0447691 50.962 416 198 1 156 565 129 544 6.35e-153 449
Msa0515820 MtrunA17_Chr2g0319091 54.324 370 169 0 196 565 210 579 3.04e-145 431
Msa0515820 MtrunA17_Chr3g0101141 49.606 381 191 1 186 565 162 542 1.67e-139 415
Msa0515820 MtrunA17_Chr5g0445331 51.036 386 184 3 185 565 147 532 1.29e-137 410
Msa0515820 MtrunA17_Chr4g0076091 47.568 370 193 1 196 564 173 542 2.47e-126 381
Msa0515820 MtrunA17_Chr2g0290241 47.170 371 195 1 196 565 55 425 2.51e-124 372
Msa0515820 MtrunA17_Chr7g0236851 38.767 454 255 10 119 564 218 656 7.57e-87 282
Msa0515820 MtrunA17_Chr6g0468561 40.409 391 191 10 196 565 293 662 1.82e-80 265
Msa0515820 MtrunA17_Chr7g0224271 37.726 387 211 5 197 565 299 673 1.08e-76 256
Msa0515820 MtrunA17_Chr3g0110971 34.987 383 218 8 199 565 220 587 1.43e-66 227
Msa0515820 MtrunA17_Chr8g0376381 35.657 373 214 10 199 565 170 522 2.19e-65 222
Msa0515820 MtrunA17_Chr8g0357601 34.908 381 223 7 199 565 183 552 1.71e-64 220
Msa0515820 MtrunA17_Chr2g0306641 33.830 470 272 18 115 565 38 487 1.70e-63 216
Msa0515820 MtrunA17_Chr4g0031721 31.892 370 251 1 196 564 314 683 4.46e-63 219
Msa0515820 MtrunA17_Chr7g0245601 36.339 366 218 6 201 565 441 792 3.86e-62 219
Msa0515820 MtrunA17_Chr4g0057891 32.723 382 235 5 197 565 148 520 4.56e-61 210
Msa0515820 MtrunA17_Chr7g0238891 30.479 397 269 2 176 565 344 740 1.92e-59 210
Msa0515820 MtrunA17_Chr4g0031741 31.707 369 247 4 198 564 284 649 7.73e-58 204
Msa0515820 MtrunA17_Chr2g0328381 30.108 372 253 3 196 564 273 640 2.04e-56 200
Msa0515820 MtrunA17_Chr2g0328341 30.376 372 252 3 196 564 270 637 3.20e-56 199
Msa0515820 MtrunA17_Chr4g0038851 28.791 455 253 8 171 565 26 469 3.76e-56 196
Msa0515820 MtrunA17_Chr4g0038761 35.310 371 219 10 201 565 87 442 3.51e-55 192
Msa0515820 MtrunA17_Chr4g0031711 29.144 374 260 3 196 564 359 732 3.95e-55 198
Msa0515820 MtrunA17_Chr4g0031701 28.054 442 303 5 130 562 188 623 1.18e-54 195
Msa0515820 MtrunA17_Chr4g0057291 35.602 382 219 14 198 564 292 661 1.62e-54 196
Msa0515820 MtrunA17_Chr2g0328391 30.585 376 250 7 196 564 297 668 2.89e-54 195
Msa0515820 MtrunA17_Chr4g0031731 28.217 443 303 5 130 563 188 624 3.83e-54 194
Msa0515820 MtrunA17_Chr5g0396491 28.605 430 240 7 197 565 55 478 4.07e-54 191
Msa0515820 MtrunA17_Chr3g0085681 28.504 421 245 5 195 565 22 436 9.78e-52 183
Msa0515820 MtrunA17_Chr4g0039661 32.181 376 232 8 199 565 194 555 9.84e-51 183
Msa0515820 MtrunA17_Chr2g0328411 28.385 384 269 4 182 564 275 653 3.11e-50 184
Msa0515820 MtrunA17_Chr5g0401131 31.486 397 247 10 185 565 103 490 3.79e-50 180
Msa0515820 MtrunA17_Chr2g0328431 27.097 465 311 9 117 564 200 653 1.35e-48 179
Msa0515820 MtrunA17_Chr3g0114841 31.863 408 237 12 185 564 105 499 6.68e-48 174
Msa0515820 MtrunA17_Chr2g0328401 30.000 340 229 5 198 534 290 623 1.29e-46 173
Msa0515820 MtrunA17_Chr1g0200401 29.471 397 236 12 197 565 79 459 9.30e-46 167
Msa0515820 MtrunA17_Chr2g0328361 30.295 373 226 8 196 564 272 614 2.20e-45 169
Msa0515820 MtrunA17_Chr1g0183641 32.181 376 224 11 198 564 123 476 9.15e-45 165
Msa0515820 MtrunA17_Chr5g0401421 27.962 422 242 8 196 564 33 445 2.63e-43 160
Msa0515820 MtrunA17_Chr2g0329591 29.049 389 246 8 197 565 73 451 3.10e-41 154
Msa0515820 MtrunA17_Chr4g0053901 26.835 436 273 7 174 564 33 467 8.04e-39 148
Msa0515820 MtrunA17_Chr5g0424131 30.548 383 247 10 197 564 120 498 1.59e-38 148
Msa0515820 MtrunA17_Chr3g0085361 26.934 349 199 4 266 565 9 350 1.01e-37 142
Msa0515820 MtrunA17_Chr2g0328371 31.474 251 167 2 269 518 2 248 1.19e-37 139
Msa0515820 MtrunA17_Chr2g0294461 26.098 410 263 10 178 565 194 585 1.63e-30 125
Msa0515820 MtrunA17_Chr7g0230731 27.688 372 248 8 199 565 373 728 4.93e-29 122
Msa0515820 MtrunA17_Chr7g0230771 27.957 372 247 8 199 565 376 731 1.72e-28 120
Msa0515820 MtrunA17_Chr7g0270031 26.649 379 256 10 197 565 188 554 6.75e-27 114
Msa0515820 MtrunA17_Chr2g0294471 25.455 385 256 10 199 565 205 576 9.98e-27 114
Msa0515820 MtrunA17_Chr8g0383981 27.441 379 241 12 198 564 139 495 5.00e-25 108
Msa0515820 MtrunA17_Chr4g0012071 25.995 377 247 11 199 565 197 551 7.11e-24 105
Msa0515820 MtrunA17_Chr2g0323111 29.310 174 121 2 392 564 133 305 1.07e-20 93.2
Msa0515820 MtrunA17_Chr1g0194621 27.875 287 185 9 287 565 246 518 6.79e-20 93.2
Msa0515820 MtrunA17_Chr8g0344101 23.077 403 266 15 190 565 215 600 1.03e-19 92.8
Msa0515820 MtrunA17_Chr8g0373561 21.891 402 236 14 199 565 183 541 6.65e-13 71.2
Msa0515820 MtrunA17_Chr4g0037781 32.558 172 89 6 171 337 39 188 4.36e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515820 AT1G21450.1 59.496 595 194 16 1 565 16 593 0.0 644
Msa0515820 AT5G48150.1 52.911 395 183 2 172 565 98 490 2.75e-146 431
Msa0515820 AT5G48150.4 52.911 395 183 2 172 565 98 490 2.75e-146 431
Msa0515820 AT5G48150.2 52.911 395 183 2 172 565 98 490 2.75e-146 431
Msa0515820 AT5G48150.3 52.911 395 183 2 172 565 98 490 2.75e-146 431
Msa0515820 AT1G50600.3 51.948 385 184 1 182 565 142 526 7.19e-142 421
Msa0515820 AT1G50600.1 51.948 385 184 1 182 565 213 597 5.98e-141 421
Msa0515820 AT1G50600.2 51.948 385 184 1 182 565 217 601 9.98e-141 421
Msa0515820 AT2G04890.1 51.892 370 171 1 196 565 51 413 4.65e-135 399
Msa0515820 AT4G17230.1 47.027 370 196 0 196 565 156 525 3.17e-124 375
Msa0515820 AT4G17230.2 47.027 370 196 0 196 565 156 525 3.17e-124 375
Msa0515820 AT5G52510.1 39.481 385 208 5 196 565 266 640 4.10e-85 277
Msa0515820 AT1G66350.1 37.097 372 208 9 199 565 156 506 2.86e-69 232
Msa0515820 AT3G03450.1 36.340 377 215 9 199 565 184 545 9.00e-68 229
Msa0515820 AT5G17490.1 35.242 454 246 13 136 565 87 516 2.50e-67 227
Msa0515820 AT3G54220.1 36.856 369 213 8 201 564 296 649 4.47e-66 227
Msa0515820 AT1G07530.1 33.760 391 254 3 176 564 377 764 2.22e-64 224
Msa0515820 AT5G66770.1 36.340 377 217 9 199 565 221 584 1.99e-60 210
Msa0515820 AT1G14920.1 33.780 373 225 10 199 565 173 529 1.68e-59 207
Msa0515820 AT2G01570.1 34.301 379 215 12 199 565 225 581 2.98e-58 204
Msa0515820 AT2G29060.2 31.980 394 252 5 179 564 269 654 2.09e-57 204
Msa0515820 AT2G29060.1 31.980 394 252 5 179 564 306 691 3.01e-57 204
Msa0515820 AT2G37650.2 29.919 371 257 2 196 565 333 701 1.78e-56 202
Msa0515820 AT2G37650.1 29.919 371 257 2 196 565 345 713 1.94e-56 202
Msa0515820 AT5G59450.1 29.777 403 271 5 172 565 199 598 5.42e-56 199
Msa0515820 AT2G29065.1 30.423 378 254 5 196 564 250 627 9.86e-54 193
Msa0515820 AT3G46600.2 29.919 371 258 2 196 565 2 371 1.17e-53 187
Msa0515820 AT3G46600.1 29.919 371 258 2 196 565 210 579 1.95e-52 189
Msa0515820 AT3G46600.3 29.919 371 258 2 196 565 178 547 2.07e-52 188
Msa0515820 AT5G41920.1 32.718 379 223 10 198 565 43 400 1.96e-51 182
Msa0515820 AT3G50650.1 33.983 359 209 7 221 565 198 542 1.80e-50 182
Msa0515820 AT1G07520.2 27.820 399 272 5 182 564 130 528 7.10e-50 181
Msa0515820 AT1G07520.4 27.820 399 272 5 182 564 130 528 7.10e-50 181
Msa0515820 AT1G07520.1 27.820 399 272 5 182 564 294 692 1.32e-49 182
Msa0515820 AT1G07520.3 27.820 399 272 5 182 564 295 693 1.32e-49 182
Msa0515820 AT4G37650.1 29.206 428 259 11 165 565 119 529 1.60e-48 177
Msa0515820 AT1G63100.1 31.362 389 233 8 198 564 252 628 1.46e-43 164
Msa0515820 AT1G63100.2 33.141 347 212 7 198 536 277 611 1.64e-43 165
Msa0515820 AT4G08250.1 29.825 342 220 7 233 564 145 476 4.97e-42 158
Msa0515820 AT1G55580.1 27.119 413 249 9 196 565 42 445 1.99e-40 152
Msa0515820 AT3G49950.1 27.720 386 256 9 196 564 28 407 8.57e-39 147
Msa0515820 AT1G50420.1 32.857 210 132 2 197 403 56 259 1.17e-30 125
Msa0515820 AT1G50420.1 37.748 151 91 2 417 565 330 479 9.52e-28 117
Msa0515820 AT3G13840.1 26.000 400 267 11 178 565 128 510 4.61e-22 100
Msa0515820 AT3G60630.1 25.501 349 229 10 231 565 291 622 3.74e-21 97.8
Msa0515820 AT2G45160.1 26.214 309 202 8 268 565 346 639 5.74e-21 97.4
Msa0515820 AT4G00150.1 24.069 349 234 10 222 565 232 554 9.99e-19 90.1
Msa0515820 AT5G67411.1 33.668 199 119 6 315 502 2 198 6.00e-18 82.8
Msa0515820 AT4G36710.1 23.864 352 244 9 222 565 150 485 3.38e-16 82.0

Find 116 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTATGAGAATAAGAAATT+TGG 0.178504 3_4:-67190626 None:intergenic
GGTACAACCATGTCATTAAT+TGG 0.248675 3_4:+67190519 None:intergenic
CCATGTAGGCTGCAATTCTT+TGG 0.255636 3_4:-67191223 None:intergenic
GAAAAGGGTGCACTTCATTT+TGG 0.257256 3_4:+67192160 Msa0515820:CDS
CACCAAATCTGATCCGAAAT+TGG 0.280682 3_4:-67190796 None:intergenic
GGTATGAAGTCGCGGGAAAA+TGG 0.287860 3_4:+67192026 Msa0515820:CDS
GCTACTGATGCTGCTAATAT+TGG 0.307471 3_4:-67191054 None:intergenic
TCCACGACCGTTACAAGTTT+TGG 0.308347 3_4:-67191809 None:intergenic
AAGAAATAACGAAAATTTCT+AGG 0.316566 3_4:+67191084 Msa0515820:CDS
GTCCAATTTCGGATCAGATT+TGG 0.328151 3_4:+67190794 Msa0515820:CDS
TCGAAGCAAGTGTTTGAATC+AGG 0.332729 3_4:-67191481 None:intergenic
TTGATCCTAACATTATTGAT+TGG 0.340862 3_4:+67190955 Msa0515820:CDS
CTTATGAGAATAAGAAATTT+GGG 0.359390 3_4:-67190625 None:intergenic
TCAGCACCACGTTGCCTTGT+TGG 0.374411 3_4:-67190768 None:intergenic
GGTGCTGATCAGTCCAATTT+CGG 0.378562 3_4:+67190783 Msa0515820:CDS
TCACTCACATTTGTGCTCAT+TGG 0.383131 3_4:-67192085 None:intergenic
GTACAAGATAAAGGAGGAAA+AGG 0.383813 3_4:+67192144 Msa0515820:CDS
GCCGCTCGCCTCGCTGAATC+CGG 0.387906 3_4:+67191257 Msa0515820:CDS
ATTGAGGAAGAAGAGGAATA+TGG 0.391911 3_4:+67190912 Msa0515820:CDS
TGATCCTAACATTATTGATT+GGG 0.392056 3_4:+67190956 Msa0515820:CDS
CGACGAATTGTCTCAATTCA+AGG 0.402603 3_4:+67191191 Msa0515820:CDS
GGCAAGGGATATTGTTAATA+CGG 0.403210 3_4:+67191973 Msa0515820:CDS
CATTCATAAAGAAGTTGCTT+GGG 0.414263 3_4:-67191110 None:intergenic
AACAGCAGCATATTCTGAAT+TGG 0.421315 3_4:-67190990 None:intergenic
CTGTGATAGGTACAAGATAA+AGG 0.425096 3_4:+67192135 Msa0515820:CDS
GGGTTAACATCGATTTCCTT+TGG 0.428305 3_4:-67190591 None:intergenic
GTGAATTCAATTGAAGTTGA+TGG 0.432801 3_4:+67190834 Msa0515820:CDS
TCAGCCCAATCAATAATGTT+AGG 0.434921 3_4:-67190960 None:intergenic
ACCGTTGAAACGCTCTCGTC+AGG 0.440496 3_4:-67191743 None:intergenic
CCATCACATGTTAGATTGAC+CGG 0.440788 3_4:+67191515 Msa0515820:CDS
ATGCTGCTAATATTGGAATC+TGG 0.446519 3_4:-67191047 None:intergenic
CATGTAGGCTGCAATTCTTT+GGG 0.448246 3_4:-67191222 None:intergenic
ATGCAAGGAACCTCCTTCTT+CGG 0.450058 3_4:+67191304 Msa0515820:CDS
AAGTTAAGTTGTACTGTGAT+AGG 0.450787 3_4:+67192122 Msa0515820:CDS
CCAAAGAATTGCAGCCTACA+TGG 0.451697 3_4:+67191223 Msa0515820:CDS
GAGATCAAAACTAGAAGCAT+TGG 0.459866 3_4:+67190863 Msa0515820:CDS
ACTTGATTGTTGCTTCAGCT+TGG 0.468732 3_4:+67192197 Msa0515820:CDS
TCTGGAGACGAATACTCCTT+TGG 0.473491 3_4:-67191029 None:intergenic
TTCGGATCAGATTTGGTGAT+GGG 0.474390 3_4:+67190801 Msa0515820:CDS
TGATTCAAACACTTGCTTCG+AGG 0.476019 3_4:+67191483 Msa0515820:CDS
TACTCTTCCTAGAGAGAGCC+AGG 0.477900 3_4:+67191922 Msa0515820:CDS
AGCATATTCTGAATTGGATC+AGG 0.480387 3_4:-67190984 None:intergenic
CGTCAGGCATGTGATGAAGC+TGG 0.484030 3_4:-67191727 None:intergenic
TTGCATGTGAAGGTGAAGAT+AGG 0.487421 3_4:+67191996 Msa0515820:CDS
TCTCAACATTCATCCTATCC+TGG 0.491515 3_4:-67191940 None:intergenic
GTTGGAGGACTAAAGATCAT+AGG 0.492091 3_4:+67191569 Msa0515820:CDS
AGCGATGCAGATACTGTTCG+AGG 0.503528 3_4:+67191337 Msa0515820:CDS
ACGAGACCAACTTCTTCGCT+TGG 0.503960 3_4:+67191772 Msa0515820:CDS
TGATTGTTGCTTCAGCTTGG+AGG 0.503995 3_4:+67192200 Msa0515820:CDS
ACATTCATAAAGAAGTTGCT+TGG 0.509000 3_4:-67191111 None:intergenic
ATCCTATCCTGGCTCTCTCT+AGG 0.512100 3_4:-67191929 None:intergenic
TCTTTACCAAGCGAAGAAGT+TGG 0.520115 3_4:-67191778 None:intergenic
TCGCGGGAAAATGGAGAGCT+AGG 0.521293 3_4:+67192035 Msa0515820:CDS
GCTGCTAGACGGTCCGAAGA+AGG 0.532544 3_4:-67191317 None:intergenic
TCTGTGCAGCGATCCGTTGG+AGG 0.533052 3_4:+67191554 Msa0515820:CDS
CGAAGCAAGTGTTTGAATCA+GGG 0.537450 3_4:-67191480 None:intergenic
GTAGAGATGTCAGAACTAGA+TGG 0.537559 3_4:-67190735 None:intergenic
GAGTCTGTGCAGCGATCCGT+TGG 0.538684 3_4:+67191551 Msa0515820:CDS
TCCAAAACTTGTAACGGTCG+TGG 0.541218 3_4:+67191808 Msa0515820:CDS
TTATAGAGCTTTGAAATGCA+AGG 0.546542 3_4:+67191289 Msa0515820:CDS
TACAAGATAAAGGAGGAAAA+GGG 0.547340 3_4:+67192145 Msa0515820:CDS
AGAGCTAGGATGACAATGGC+TGG 0.548519 3_4:+67192049 Msa0515820:CDS
TTTGAATCCAAAACTTGTAA+CGG 0.552516 3_4:+67191802 Msa0515820:CDS
TAGTGACATTGAGGAAGAAG+AGG 0.556415 3_4:+67190905 Msa0515820:CDS
ACGGATCGCTGCACAGACTC+AGG 0.557758 3_4:-67191548 None:intergenic
GAACAACTAGCAGAAACACT+TGG 0.558635 3_4:+67191602 Msa0515820:CDS
ACTTGATGATAGTGACATTG+AGG 0.558721 3_4:+67190896 Msa0515820:CDS
TTTCGGATCAGATTTGGTGA+TGG 0.559466 3_4:+67190800 Msa0515820:CDS
ATATGGTAATTCTCAAAGCA+TGG 0.562265 3_4:+67190929 Msa0515820:CDS
CCGGTCAATCTAACATGTGA+TGG 0.566500 3_4:-67191515 None:intergenic
CATCACATGTTAGATTGACC+GGG 0.567991 3_4:+67191516 Msa0515820:CDS
CTCGTAACTCTCGCTCGAGT+AGG 0.572630 3_4:-67190673 None:intergenic
TTGAGAGGCAATGCTTGGCA+AGG 0.577561 3_4:+67191957 Msa0515820:CDS
AATTGCAGCCTACATGGTGG+AGG 0.577669 3_4:+67191229 Msa0515820:CDS
TAGGCTGCAATTCTTTGGGA+AGG 0.583102 3_4:-67191218 None:intergenic
CAAAACTAGAAGCATTGGAG+AGG 0.583413 3_4:+67190868 Msa0515820:CDS
GAATGTTGAGAGGCAATGCT+TGG 0.583862 3_4:+67191952 Msa0515820:CDS
CTACAAGTTTCATGCCAACA+AGG 0.584293 3_4:+67190754 Msa0515820:CDS
TGTGCTACTGCAATTTCAGA+AGG 0.584377 3_4:+67191131 Msa0515820:CDS
GCCTGACGAGAGCGTTTCAA+CGG 0.590858 3_4:+67191742 Msa0515820:CDS
TCCGGATTCAGCGAGGCGAG+CGG 0.592435 3_4:-67191258 None:intergenic
AATACTCCTTTGGTGAGTCA+TGG 0.604923 3_4:-67191019 None:intergenic
CACTAAGCTCAAATCGCCAA+AGG 0.608012 3_4:+67190575 Msa0515820:CDS
TATGATCTTTAGTCCTCCAA+CGG 0.609253 3_4:-67191567 None:intergenic
GTATCTGCATCGCTGCTAGA+CGG 0.609923 3_4:-67191328 None:intergenic
ATTCATAAAGAAGTTGCTTG+GGG 0.610084 3_4:-67191109 None:intergenic
GTTAATACGGTTGCATGTGA+AGG 0.612068 3_4:+67191986 Msa0515820:CDS
ATAGAACGGTATGAAGTCGC+GGG 0.612894 3_4:+67192019 Msa0515820:CDS
AGACTCAGGATCATCAATCC+CGG 0.614792 3_4:-67191534 None:intergenic
TAACTATGCAATAACAGAAG+CGG 0.616342 3_4:+67191394 Msa0515820:CDS
GATAGAACGGTATGAAGTCG+CGG 0.616637 3_4:+67192018 Msa0515820:CDS
TCGTCGTGGAGAAGCTCTTG+TGG 0.619304 3_4:+67191691 Msa0515820:CDS
CCTTTGGTGAGTCATGGTCA+TGG 0.621918 3_4:-67191013 None:intergenic
AAGGTGAAGATAGGATAGAA+CGG 0.625100 3_4:+67192005 Msa0515820:CDS
TTCCTAGAGAGAGCCAGGAT+AGG 0.625909 3_4:+67191927 Msa0515820:CDS
TGAGAGGCAATGCTTGGCAA+GGG 0.635494 3_4:+67191958 Msa0515820:CDS
TCGATGCTTGATTGTCGTCG+TGG 0.642078 3_4:+67191677 Msa0515820:CDS
AAAACTAGAAGCATTGGAGA+GGG 0.649054 3_4:+67190869 Msa0515820:CDS
AGAAGATCCAATTAATGACA+TGG 0.650375 3_4:-67190526 None:intergenic
ATTGCAGCCTACATGGTGGA+GGG 0.650477 3_4:+67191230 Msa0515820:CDS
GGCGAGTCCCTCCACCATGT+AGG 0.652264 3_4:-67191237 None:intergenic
GCTAGACGGTCCGAAGAAGG+AGG 0.655079 3_4:-67191314 None:intergenic
TTCATAAAGAAGTTGCTTGG+GGG 0.656482 3_4:-67191108 None:intergenic
ATGAGAATAAGAAATTTGGG+AGG 0.658872 3_4:-67190622 None:intergenic
AGGATAGGATGAATGTTGAG+AGG 0.660140 3_4:+67191942 Msa0515820:CDS
AATTTCAGAAGGAAATGAAG+AGG 0.662867 3_4:+67191142 Msa0515820:CDS
ATGGAGAGCTAGGATGACAA+TGG 0.667953 3_4:+67192045 Msa0515820:CDS
CCATGACCATGACTCACCAA+AGG 0.671972 3_4:+67191013 Msa0515820:CDS
CTTGAATTGAGACAATTCGT+CGG 0.672967 3_4:-67191190 None:intergenic
AATTTCGAGCGGTCTCATCG+AGG 0.679065 3_4:+67191636 Msa0515820:CDS
TTTGTCATTTGAATTTCGAG+CGG 0.680630 3_4:+67191625 Msa0515820:CDS
AAGAATTGCAGCCTACATGG+TGG 0.682524 3_4:+67191226 Msa0515820:CDS
TGATAGGTACAAGATAAAGG+AGG 0.692576 3_4:+67192138 Msa0515820:CDS
ACGACAATCAAGCATCGATG+AGG 0.692866 3_4:-67191672 None:intergenic
ATAAACCCGAATTTAAAACA+CGG 0.716869 3_4:-67191365 None:intergenic
TTCATGCCAACAAGGCAACG+TGG 0.722675 3_4:+67190762 Msa0515820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTATGAGAATAAGAAATTT+GGG - chr3_4:67190628-67190647 None:intergenic 20.0%
!!! AGAAATTTTCGTTATTTCTT+TGG - chr3_4:67191084-67191103 None:intergenic 20.0%
!! AAGAAATAACGAAAATTTCT+AGG + chr3_4:67191084-67191103 Msa0515820:CDS 20.0%
!!! ATAGATTTTGATATCAATCA+AGG + chr3_4:67191443-67191462 Msa0515820:CDS 20.0%
!! CTTATGAGAATAAGAAATTT+GGG - chr3_4:67190628-67190647 None:intergenic 20.0%
!!! AGAAATTTTCGTTATTTCTT+TGG - chr3_4:67191084-67191103 None:intergenic 20.0%
!! AAGAAATAACGAAAATTTCT+AGG + chr3_4:67191084-67191103 Msa0515820:CDS 20.0%
!!! ATAGATTTTGATATCAATCA+AGG + chr3_4:67191443-67191462 Msa0515820:CDS 20.0%
! GCTTATGAGAATAAGAAATT+TGG - chr3_4:67190629-67190648 None:intergenic 25.0%
! TTGATCCTAACATTATTGAT+TGG + chr3_4:67190955-67190974 Msa0515820:CDS 25.0%
!! TGATCCTAACATTATTGATT+GGG + chr3_4:67190956-67190975 Msa0515820:CDS 25.0%
!!! AATTTTCGTTATTTCTTTGG+TGG - chr3_4:67191081-67191100 None:intergenic 25.0%
!!! AGCTCTATAAATACTTTTTC+CGG - chr3_4:67191279-67191298 None:intergenic 25.0%
!! ATAAACCCGAATTTAAAACA+CGG - chr3_4:67191368-67191387 None:intergenic 25.0%
! TTTGAATCCAAAACTTGTAA+CGG + chr3_4:67191802-67191821 Msa0515820:CDS 25.0%
!!! AGTACAACTTAACTTGTTTT+CGG - chr3_4:67192117-67192136 None:intergenic 25.0%
! GCTTATGAGAATAAGAAATT+TGG - chr3_4:67190629-67190648 None:intergenic 25.0%
! TTGATCCTAACATTATTGAT+TGG + chr3_4:67190955-67190974 Msa0515820:CDS 25.0%
!! TGATCCTAACATTATTGATT+GGG + chr3_4:67190956-67190975 Msa0515820:CDS 25.0%
!!! AATTTTCGTTATTTCTTTGG+TGG - chr3_4:67191081-67191100 None:intergenic 25.0%
!!! AGCTCTATAAATACTTTTTC+CGG - chr3_4:67191279-67191298 None:intergenic 25.0%
!! ATAAACCCGAATTTAAAACA+CGG - chr3_4:67191368-67191387 None:intergenic 25.0%
! TTTGAATCCAAAACTTGTAA+CGG + chr3_4:67191802-67191821 Msa0515820:CDS 25.0%
!!! AGTACAACTTAACTTGTTTT+CGG - chr3_4:67192117-67192136 None:intergenic 25.0%
AAATTTGGGAGGAAAAATCA+GGG - chr3_4:67190614-67190633 None:intergenic 30.0%
ATGAGAATAAGAAATTTGGG+AGG - chr3_4:67190625-67190644 None:intergenic 30.0%
GTGAATTCAATTGAAGTTGA+TGG + chr3_4:67190834-67190853 Msa0515820:CDS 30.0%
ATATGGTAATTCTCAAAGCA+TGG + chr3_4:67190929-67190948 Msa0515820:CDS 30.0%
ATTCATAAAGAAGTTGCTTG+GGG - chr3_4:67191112-67191131 None:intergenic 30.0%
CATTCATAAAGAAGTTGCTT+GGG - chr3_4:67191113-67191132 None:intergenic 30.0%
ACATTCATAAAGAAGTTGCT+TGG - chr3_4:67191114-67191133 None:intergenic 30.0%
AATTTCAGAAGGAAATGAAG+AGG + chr3_4:67191142-67191161 Msa0515820:CDS 30.0%
!! TTATAGAGCTTTGAAATGCA+AGG + chr3_4:67191289-67191308 Msa0515820:CDS 30.0%
!! GTTTGTCCGTGTTTTAAATT+CGG + chr3_4:67191359-67191378 Msa0515820:CDS 30.0%
!! TTTGTCCGTGTTTTAAATTC+GGG + chr3_4:67191360-67191379 Msa0515820:CDS 30.0%
TAACTATGCAATAACAGAAG+CGG + chr3_4:67191394-67191413 Msa0515820:CDS 30.0%
! TTTGTCATTTGAATTTCGAG+CGG + chr3_4:67191625-67191644 Msa0515820:CDS 30.0%
TCAACAAATCTCTGCAAAAA+CGG - chr3_4:67191860-67191879 None:intergenic 30.0%
! AAGTTAAGTTGTACTGTGAT+AGG + chr3_4:67192122-67192141 Msa0515820:CDS 30.0%
TACAAGATAAAGGAGGAAAA+GGG + chr3_4:67192145-67192164 Msa0515820:CDS 30.0%
AAATTTGGGAGGAAAAATCA+GGG - chr3_4:67190614-67190633 None:intergenic 30.0%
ATGAGAATAAGAAATTTGGG+AGG - chr3_4:67190625-67190644 None:intergenic 30.0%
GTGAATTCAATTGAAGTTGA+TGG + chr3_4:67190834-67190853 Msa0515820:CDS 30.0%
ATATGGTAATTCTCAAAGCA+TGG + chr3_4:67190929-67190948 Msa0515820:CDS 30.0%
ATTCATAAAGAAGTTGCTTG+GGG - chr3_4:67191112-67191131 None:intergenic 30.0%
CATTCATAAAGAAGTTGCTT+GGG - chr3_4:67191113-67191132 None:intergenic 30.0%
ACATTCATAAAGAAGTTGCT+TGG - chr3_4:67191114-67191133 None:intergenic 30.0%
AATTTCAGAAGGAAATGAAG+AGG + chr3_4:67191142-67191161 Msa0515820:CDS 30.0%
!! TTATAGAGCTTTGAAATGCA+AGG + chr3_4:67191289-67191308 Msa0515820:CDS 30.0%
!! GTTTGTCCGTGTTTTAAATT+CGG + chr3_4:67191359-67191378 Msa0515820:CDS 30.0%
!! TTTGTCCGTGTTTTAAATTC+GGG + chr3_4:67191360-67191379 Msa0515820:CDS 30.0%
TAACTATGCAATAACAGAAG+CGG + chr3_4:67191394-67191413 Msa0515820:CDS 30.0%
! TTTGTCATTTGAATTTCGAG+CGG + chr3_4:67191625-67191644 Msa0515820:CDS 30.0%
TCAACAAATCTCTGCAAAAA+CGG - chr3_4:67191860-67191879 None:intergenic 30.0%
! AAGTTAAGTTGTACTGTGAT+AGG + chr3_4:67192122-67192141 Msa0515820:CDS 30.0%
TACAAGATAAAGGAGGAAAA+GGG + chr3_4:67192145-67192164 Msa0515820:CDS 30.0%
! TTAGTGCTTTTATGTGATGG+TGG - chr3_4:67190561-67190580 None:intergenic 35.0%
! AGCTTAGTGCTTTTATGTGA+TGG - chr3_4:67190564-67190583 None:intergenic 35.0%
GAAATTTGGGAGGAAAAATC+AGG - chr3_4:67190615-67190634 None:intergenic 35.0%
! GAGATCAAAACTAGAAGCAT+TGG + chr3_4:67190863-67190882 Msa0515820:CDS 35.0%
!! AAAACTAGAAGCATTGGAGA+GGG + chr3_4:67190869-67190888 Msa0515820:CDS 35.0%
! ACTTGATGATAGTGACATTG+AGG + chr3_4:67190896-67190915 Msa0515820:CDS 35.0%
ATTGAGGAAGAAGAGGAATA+TGG + chr3_4:67190912-67190931 Msa0515820:CDS 35.0%
TCAGCCCAATCAATAATGTT+AGG - chr3_4:67190963-67190982 None:intergenic 35.0%
AGCATATTCTGAATTGGATC+AGG - chr3_4:67190987-67191006 None:intergenic 35.0%
AACAGCAGCATATTCTGAAT+TGG - chr3_4:67190993-67191012 None:intergenic 35.0%
ATGCTGCTAATATTGGAATC+TGG - chr3_4:67191050-67191069 None:intergenic 35.0%
TTCATAAAGAAGTTGCTTGG+GGG - chr3_4:67191111-67191130 None:intergenic 35.0%
CTTGAATTGAGACAATTCGT+CGG - chr3_4:67191193-67191212 None:intergenic 35.0%
TATGATCTTTAGTCCTCCAA+CGG - chr3_4:67191570-67191589 None:intergenic 35.0%
AACAAATCTCTGCAAAAACG+GGG - chr3_4:67191858-67191877 None:intergenic 35.0%
CAACAAATCTCTGCAAAAAC+GGG - chr3_4:67191859-67191878 None:intergenic 35.0%
GGCAAGGGATATTGTTAATA+CGG + chr3_4:67191973-67191992 Msa0515820:CDS 35.0%
AAGGTGAAGATAGGATAGAA+CGG + chr3_4:67192005-67192024 Msa0515820:CDS 35.0%
CTGTGATAGGTACAAGATAA+AGG + chr3_4:67192135-67192154 Msa0515820:CDS 35.0%
TGATAGGTACAAGATAAAGG+AGG + chr3_4:67192138-67192157 Msa0515820:CDS 35.0%
GTACAAGATAAAGGAGGAAA+AGG + chr3_4:67192144-67192163 Msa0515820:CDS 35.0%
! TTAGTGCTTTTATGTGATGG+TGG - chr3_4:67190561-67190580 None:intergenic 35.0%
! AGCTTAGTGCTTTTATGTGA+TGG - chr3_4:67190564-67190583 None:intergenic 35.0%
GAAATTTGGGAGGAAAAATC+AGG - chr3_4:67190615-67190634 None:intergenic 35.0%
! GAGATCAAAACTAGAAGCAT+TGG + chr3_4:67190863-67190882 Msa0515820:CDS 35.0%
!! AAAACTAGAAGCATTGGAGA+GGG + chr3_4:67190869-67190888 Msa0515820:CDS 35.0%
! ACTTGATGATAGTGACATTG+AGG + chr3_4:67190896-67190915 Msa0515820:CDS 35.0%
ATTGAGGAAGAAGAGGAATA+TGG + chr3_4:67190912-67190931 Msa0515820:CDS 35.0%
TCAGCCCAATCAATAATGTT+AGG - chr3_4:67190963-67190982 None:intergenic 35.0%
AGCATATTCTGAATTGGATC+AGG - chr3_4:67190987-67191006 None:intergenic 35.0%
AACAGCAGCATATTCTGAAT+TGG - chr3_4:67190993-67191012 None:intergenic 35.0%
ATGCTGCTAATATTGGAATC+TGG - chr3_4:67191050-67191069 None:intergenic 35.0%
TTCATAAAGAAGTTGCTTGG+GGG - chr3_4:67191111-67191130 None:intergenic 35.0%
CTTGAATTGAGACAATTCGT+CGG - chr3_4:67191193-67191212 None:intergenic 35.0%
TATGATCTTTAGTCCTCCAA+CGG - chr3_4:67191570-67191589 None:intergenic 35.0%
AACAAATCTCTGCAAAAACG+GGG - chr3_4:67191858-67191877 None:intergenic 35.0%
CAACAAATCTCTGCAAAAAC+GGG - chr3_4:67191859-67191878 None:intergenic 35.0%
GGCAAGGGATATTGTTAATA+CGG + chr3_4:67191973-67191992 Msa0515820:CDS 35.0%
AAGGTGAAGATAGGATAGAA+CGG + chr3_4:67192005-67192024 Msa0515820:CDS 35.0%
CTGTGATAGGTACAAGATAA+AGG + chr3_4:67192135-67192154 Msa0515820:CDS 35.0%
TGATAGGTACAAGATAAAGG+AGG + chr3_4:67192138-67192157 Msa0515820:CDS 35.0%
GTACAAGATAAAGGAGGAAA+AGG + chr3_4:67192144-67192163 Msa0515820:CDS 35.0%
!! GGGTTAACATCGATTTCCTT+TGG - chr3_4:67190594-67190613 None:intergenic 40.0%
GTAGAGATGTCAGAACTAGA+TGG - chr3_4:67190738-67190757 None:intergenic 40.0%
CTACAAGTTTCATGCCAACA+AGG + chr3_4:67190754-67190773 Msa0515820:CDS 40.0%
GTCCAATTTCGGATCAGATT+TGG + chr3_4:67190794-67190813 Msa0515820:CDS 40.0%
CACCAAATCTGATCCGAAAT+TGG - chr3_4:67190799-67190818 None:intergenic 40.0%
!! TTTCGGATCAGATTTGGTGA+TGG + chr3_4:67190800-67190819 Msa0515820:CDS 40.0%
!! TTCGGATCAGATTTGGTGAT+GGG + chr3_4:67190801-67190820 Msa0515820:CDS 40.0%
!! CAAAACTAGAAGCATTGGAG+AGG + chr3_4:67190868-67190887 Msa0515820:CDS 40.0%
TAGTGACATTGAGGAAGAAG+AGG + chr3_4:67190905-67190924 Msa0515820:CDS 40.0%
AATACTCCTTTGGTGAGTCA+TGG - chr3_4:67191022-67191041 None:intergenic 40.0%
GCTACTGATGCTGCTAATAT+TGG - chr3_4:67191057-67191076 None:intergenic 40.0%
! TGTGCTACTGCAATTTCAGA+AGG + chr3_4:67191131-67191150 Msa0515820:CDS 40.0%
CGACGAATTGTCTCAATTCA+AGG + chr3_4:67191191-67191210 Msa0515820:CDS 40.0%
CATGTAGGCTGCAATTCTTT+GGG - chr3_4:67191225-67191244 None:intergenic 40.0%
CGAAGCAAGTGTTTGAATCA+GGG - chr3_4:67191483-67191502 None:intergenic 40.0%
TCGAAGCAAGTGTTTGAATC+AGG - chr3_4:67191484-67191503 None:intergenic 40.0%
TGATTCAAACACTTGCTTCG+AGG + chr3_4:67191483-67191502 Msa0515820:CDS 40.0%
CCATCACATGTTAGATTGAC+CGG + chr3_4:67191515-67191534 Msa0515820:CDS 40.0%
CATCACATGTTAGATTGACC+GGG + chr3_4:67191516-67191535 Msa0515820:CDS 40.0%
GTTGGAGGACTAAAGATCAT+AGG + chr3_4:67191569-67191588 Msa0515820:CDS 40.0%
GAACAACTAGCAGAAACACT+TGG + chr3_4:67191602-67191621 Msa0515820:CDS 40.0%
TCTTTACCAAGCGAAGAAGT+TGG - chr3_4:67191781-67191800 None:intergenic 40.0%
TCTCAACATTCATCCTATCC+TGG - chr3_4:67191943-67191962 None:intergenic 40.0%
AGGATAGGATGAATGTTGAG+AGG + chr3_4:67191942-67191961 Msa0515820:CDS 40.0%
GTTAATACGGTTGCATGTGA+AGG + chr3_4:67191986-67192005 Msa0515820:CDS 40.0%
TTGCATGTGAAGGTGAAGAT+AGG + chr3_4:67191996-67192015 Msa0515820:CDS 40.0%
TCACTCACATTTGTGCTCAT+TGG - chr3_4:67192088-67192107 None:intergenic 40.0%
!! GAAAAGGGTGCACTTCATTT+TGG + chr3_4:67192160-67192179 Msa0515820:CDS 40.0%
! ACTTGATTGTTGCTTCAGCT+TGG + chr3_4:67192197-67192216 Msa0515820:CDS 40.0%
!! GGGTTAACATCGATTTCCTT+TGG - chr3_4:67190594-67190613 None:intergenic 40.0%
GTAGAGATGTCAGAACTAGA+TGG - chr3_4:67190738-67190757 None:intergenic 40.0%
CTACAAGTTTCATGCCAACA+AGG + chr3_4:67190754-67190773 Msa0515820:CDS 40.0%
GTCCAATTTCGGATCAGATT+TGG + chr3_4:67190794-67190813 Msa0515820:CDS 40.0%
CACCAAATCTGATCCGAAAT+TGG - chr3_4:67190799-67190818 None:intergenic 40.0%
!! TTTCGGATCAGATTTGGTGA+TGG + chr3_4:67190800-67190819 Msa0515820:CDS 40.0%
!! TTCGGATCAGATTTGGTGAT+GGG + chr3_4:67190801-67190820 Msa0515820:CDS 40.0%
!! CAAAACTAGAAGCATTGGAG+AGG + chr3_4:67190868-67190887 Msa0515820:CDS 40.0%
TAGTGACATTGAGGAAGAAG+AGG + chr3_4:67190905-67190924 Msa0515820:CDS 40.0%
AATACTCCTTTGGTGAGTCA+TGG - chr3_4:67191022-67191041 None:intergenic 40.0%
GCTACTGATGCTGCTAATAT+TGG - chr3_4:67191057-67191076 None:intergenic 40.0%
! TGTGCTACTGCAATTTCAGA+AGG + chr3_4:67191131-67191150 Msa0515820:CDS 40.0%
CGACGAATTGTCTCAATTCA+AGG + chr3_4:67191191-67191210 Msa0515820:CDS 40.0%
CATGTAGGCTGCAATTCTTT+GGG - chr3_4:67191225-67191244 None:intergenic 40.0%
CGAAGCAAGTGTTTGAATCA+GGG - chr3_4:67191483-67191502 None:intergenic 40.0%
TCGAAGCAAGTGTTTGAATC+AGG - chr3_4:67191484-67191503 None:intergenic 40.0%
TGATTCAAACACTTGCTTCG+AGG + chr3_4:67191483-67191502 Msa0515820:CDS 40.0%
CCATCACATGTTAGATTGAC+CGG + chr3_4:67191515-67191534 Msa0515820:CDS 40.0%
CATCACATGTTAGATTGACC+GGG + chr3_4:67191516-67191535 Msa0515820:CDS 40.0%
GTTGGAGGACTAAAGATCAT+AGG + chr3_4:67191569-67191588 Msa0515820:CDS 40.0%
GAACAACTAGCAGAAACACT+TGG + chr3_4:67191602-67191621 Msa0515820:CDS 40.0%
TCTTTACCAAGCGAAGAAGT+TGG - chr3_4:67191781-67191800 None:intergenic 40.0%
TCTCAACATTCATCCTATCC+TGG - chr3_4:67191943-67191962 None:intergenic 40.0%
AGGATAGGATGAATGTTGAG+AGG + chr3_4:67191942-67191961 Msa0515820:CDS 40.0%
GTTAATACGGTTGCATGTGA+AGG + chr3_4:67191986-67192005 Msa0515820:CDS 40.0%
TTGCATGTGAAGGTGAAGAT+AGG + chr3_4:67191996-67192015 Msa0515820:CDS 40.0%
TCACTCACATTTGTGCTCAT+TGG - chr3_4:67192088-67192107 None:intergenic 40.0%
!! GAAAAGGGTGCACTTCATTT+TGG + chr3_4:67192160-67192179 Msa0515820:CDS 40.0%
! ACTTGATTGTTGCTTCAGCT+TGG + chr3_4:67192197-67192216 Msa0515820:CDS 40.0%
CACTAAGCTCAAATCGCCAA+AGG + chr3_4:67190575-67190594 Msa0515820:CDS 45.0%
!! GGTGCTGATCAGTCCAATTT+CGG + chr3_4:67190783-67190802 Msa0515820:CDS 45.0%
TCTGGAGACGAATACTCCTT+TGG - chr3_4:67191032-67191051 None:intergenic 45.0%
! TAGGCTGCAATTCTTTGGGA+AGG - chr3_4:67191221-67191240 None:intergenic 45.0%
CCATGTAGGCTGCAATTCTT+TGG - chr3_4:67191226-67191245 None:intergenic 45.0%
CCAAAGAATTGCAGCCTACA+TGG + chr3_4:67191223-67191242 Msa0515820:CDS 45.0%
AAGAATTGCAGCCTACATGG+TGG + chr3_4:67191226-67191245 Msa0515820:CDS 45.0%
!!! TACTTTTTCCGGATTCAGCG+AGG - chr3_4:67191268-67191287 None:intergenic 45.0%
ATGCAAGGAACCTCCTTCTT+CGG + chr3_4:67191304-67191323 Msa0515820:CDS 45.0%
CCGGTCAATCTAACATGTGA+TGG - chr3_4:67191518-67191537 None:intergenic 45.0%
AGACTCAGGATCATCAATCC+CGG - chr3_4:67191537-67191556 None:intergenic 45.0%
!! ACGACAATCAAGCATCGATG+AGG - chr3_4:67191675-67191694 None:intergenic 45.0%
TCCAAAACTTGTAACGGTCG+TGG + chr3_4:67191808-67191827 Msa0515820:CDS 45.0%
! TCCACGACCGTTACAAGTTT+TGG - chr3_4:67191812-67191831 None:intergenic 45.0%
AAATCTCTGCAAAAACGGGG+CGG - chr3_4:67191855-67191874 None:intergenic 45.0%
GAATGTTGAGAGGCAATGCT+TGG + chr3_4:67191952-67191971 Msa0515820:CDS 45.0%
GATAGAACGGTATGAAGTCG+CGG + chr3_4:67192018-67192037 Msa0515820:CDS 45.0%
ATAGAACGGTATGAAGTCGC+GGG + chr3_4:67192019-67192038 Msa0515820:CDS 45.0%
ATGGAGAGCTAGGATGACAA+TGG + chr3_4:67192045-67192064 Msa0515820:CDS 45.0%
!! AGGGTGCACTTCATTTTGGT+TGG + chr3_4:67192164-67192183 Msa0515820:CDS 45.0%
!!! GGGTGCACTTCATTTTGGTT+GGG + chr3_4:67192165-67192184 Msa0515820:CDS 45.0%
! TGATTGTTGCTTCAGCTTGG+AGG + chr3_4:67192200-67192219 Msa0515820:CDS 45.0%
CACTAAGCTCAAATCGCCAA+AGG + chr3_4:67190575-67190594 Msa0515820:CDS 45.0%
!! GGTGCTGATCAGTCCAATTT+CGG + chr3_4:67190783-67190802 Msa0515820:CDS 45.0%
TCTGGAGACGAATACTCCTT+TGG - chr3_4:67191032-67191051 None:intergenic 45.0%
! TAGGCTGCAATTCTTTGGGA+AGG - chr3_4:67191221-67191240 None:intergenic 45.0%
CCATGTAGGCTGCAATTCTT+TGG - chr3_4:67191226-67191245 None:intergenic 45.0%
CCAAAGAATTGCAGCCTACA+TGG + chr3_4:67191223-67191242 Msa0515820:CDS 45.0%
AAGAATTGCAGCCTACATGG+TGG + chr3_4:67191226-67191245 Msa0515820:CDS 45.0%
!!! TACTTTTTCCGGATTCAGCG+AGG - chr3_4:67191268-67191287 None:intergenic 45.0%
ATGCAAGGAACCTCCTTCTT+CGG + chr3_4:67191304-67191323 Msa0515820:CDS 45.0%
CCGGTCAATCTAACATGTGA+TGG - chr3_4:67191518-67191537 None:intergenic 45.0%
AGACTCAGGATCATCAATCC+CGG - chr3_4:67191537-67191556 None:intergenic 45.0%
!! ACGACAATCAAGCATCGATG+AGG - chr3_4:67191675-67191694 None:intergenic 45.0%
TCCAAAACTTGTAACGGTCG+TGG + chr3_4:67191808-67191827 Msa0515820:CDS 45.0%
! TCCACGACCGTTACAAGTTT+TGG - chr3_4:67191812-67191831 None:intergenic 45.0%
AAATCTCTGCAAAAACGGGG+CGG - chr3_4:67191855-67191874 None:intergenic 45.0%
GAATGTTGAGAGGCAATGCT+TGG + chr3_4:67191952-67191971 Msa0515820:CDS 45.0%
GATAGAACGGTATGAAGTCG+CGG + chr3_4:67192018-67192037 Msa0515820:CDS 45.0%
ATAGAACGGTATGAAGTCGC+GGG + chr3_4:67192019-67192038 Msa0515820:CDS 45.0%
ATGGAGAGCTAGGATGACAA+TGG + chr3_4:67192045-67192064 Msa0515820:CDS 45.0%
!! AGGGTGCACTTCATTTTGGT+TGG + chr3_4:67192164-67192183 Msa0515820:CDS 45.0%
!!! GGGTGCACTTCATTTTGGTT+GGG + chr3_4:67192165-67192184 Msa0515820:CDS 45.0%
! TGATTGTTGCTTCAGCTTGG+AGG + chr3_4:67192200-67192219 Msa0515820:CDS 45.0%
TTCATGCCAACAAGGCAACG+TGG + chr3_4:67190762-67190781 Msa0515820:CDS 50.0%
CCTTTGGTGAGTCATGGTCA+TGG - chr3_4:67191016-67191035 None:intergenic 50.0%
CCATGACCATGACTCACCAA+AGG + chr3_4:67191013-67191032 Msa0515820:CDS 50.0%
AATTGCAGCCTACATGGTGG+AGG + chr3_4:67191229-67191248 Msa0515820:CDS 50.0%
! ATTGCAGCCTACATGGTGGA+GGG + chr3_4:67191230-67191249 Msa0515820:CDS 50.0%
GTATCTGCATCGCTGCTAGA+CGG - chr3_4:67191331-67191350 None:intergenic 50.0%
AGCGATGCAGATACTGTTCG+AGG + chr3_4:67191337-67191356 Msa0515820:CDS 50.0%
AATTTCGAGCGGTCTCATCG+AGG + chr3_4:67191636-67191655 Msa0515820:CDS 50.0%
TCGATGCTTGATTGTCGTCG+TGG + chr3_4:67191677-67191696 Msa0515820:CDS 50.0%
ACGAGACCAACTTCTTCGCT+TGG + chr3_4:67191772-67191791 Msa0515820:CDS 50.0%
TACTCTTCCTAGAGAGAGCC+AGG + chr3_4:67191922-67191941 Msa0515820:CDS 50.0%
TTCCTAGAGAGAGCCAGGAT+AGG + chr3_4:67191927-67191946 Msa0515820:CDS 50.0%
! ATCCTATCCTGGCTCTCTCT+AGG - chr3_4:67191932-67191951 None:intergenic 50.0%
TTGAGAGGCAATGCTTGGCA+AGG + chr3_4:67191957-67191976 Msa0515820:CDS 50.0%
TGAGAGGCAATGCTTGGCAA+GGG + chr3_4:67191958-67191977 Msa0515820:CDS 50.0%
GGTATGAAGTCGCGGGAAAA+TGG + chr3_4:67192026-67192045 Msa0515820:CDS 50.0%
AGAGCTAGGATGACAATGGC+TGG + chr3_4:67192049-67192068 Msa0515820:CDS 50.0%
TTCATGCCAACAAGGCAACG+TGG + chr3_4:67190762-67190781 Msa0515820:CDS 50.0%
CCTTTGGTGAGTCATGGTCA+TGG - chr3_4:67191016-67191035 None:intergenic 50.0%
CCATGACCATGACTCACCAA+AGG + chr3_4:67191013-67191032 Msa0515820:CDS 50.0%
AATTGCAGCCTACATGGTGG+AGG + chr3_4:67191229-67191248 Msa0515820:CDS 50.0%
! ATTGCAGCCTACATGGTGGA+GGG + chr3_4:67191230-67191249 Msa0515820:CDS 50.0%
GTATCTGCATCGCTGCTAGA+CGG - chr3_4:67191331-67191350 None:intergenic 50.0%
AGCGATGCAGATACTGTTCG+AGG + chr3_4:67191337-67191356 Msa0515820:CDS 50.0%
AATTTCGAGCGGTCTCATCG+AGG + chr3_4:67191636-67191655 Msa0515820:CDS 50.0%
TCGATGCTTGATTGTCGTCG+TGG + chr3_4:67191677-67191696 Msa0515820:CDS 50.0%
ACGAGACCAACTTCTTCGCT+TGG + chr3_4:67191772-67191791 Msa0515820:CDS 50.0%
TACTCTTCCTAGAGAGAGCC+AGG + chr3_4:67191922-67191941 Msa0515820:CDS 50.0%
TTCCTAGAGAGAGCCAGGAT+AGG + chr3_4:67191927-67191946 Msa0515820:CDS 50.0%
! ATCCTATCCTGGCTCTCTCT+AGG - chr3_4:67191932-67191951 None:intergenic 50.0%
TTGAGAGGCAATGCTTGGCA+AGG + chr3_4:67191957-67191976 Msa0515820:CDS 50.0%
TGAGAGGCAATGCTTGGCAA+GGG + chr3_4:67191958-67191977 Msa0515820:CDS 50.0%
GGTATGAAGTCGCGGGAAAA+TGG + chr3_4:67192026-67192045 Msa0515820:CDS 50.0%
AGAGCTAGGATGACAATGGC+TGG + chr3_4:67192049-67192068 Msa0515820:CDS 50.0%
CTCGTAACTCTCGCTCGAGT+AGG - chr3_4:67190676-67190695 None:intergenic 55.0%
TCAGCACCACGTTGCCTTGT+TGG - chr3_4:67190771-67190790 None:intergenic 55.0%
TCGTCGTGGAGAAGCTCTTG+TGG + chr3_4:67191691-67191710 Msa0515820:CDS 55.0%
CGTCAGGCATGTGATGAAGC+TGG - chr3_4:67191730-67191749 None:intergenic 55.0%
!! GCCTGACGAGAGCGTTTCAA+CGG + chr3_4:67191742-67191761 Msa0515820:CDS 55.0%
ACCGTTGAAACGCTCTCGTC+AGG - chr3_4:67191746-67191765 None:intergenic 55.0%
CTGCAAAAACGGGGCGGTAT+TGG - chr3_4:67191849-67191868 None:intergenic 55.0%
TCGCGGGAAAATGGAGAGCT+AGG + chr3_4:67192035-67192054 Msa0515820:CDS 55.0%
CTCGTAACTCTCGCTCGAGT+AGG - chr3_4:67190676-67190695 None:intergenic 55.0%
TCAGCACCACGTTGCCTTGT+TGG - chr3_4:67190771-67190790 None:intergenic 55.0%
TCGTCGTGGAGAAGCTCTTG+TGG + chr3_4:67191691-67191710 Msa0515820:CDS 55.0%
CGTCAGGCATGTGATGAAGC+TGG - chr3_4:67191730-67191749 None:intergenic 55.0%
!! GCCTGACGAGAGCGTTTCAA+CGG + chr3_4:67191742-67191761 Msa0515820:CDS 55.0%
ACCGTTGAAACGCTCTCGTC+AGG - chr3_4:67191746-67191765 None:intergenic 55.0%
CTGCAAAAACGGGGCGGTAT+TGG - chr3_4:67191849-67191868 None:intergenic 55.0%
TCGCGGGAAAATGGAGAGCT+AGG + chr3_4:67192035-67192054 Msa0515820:CDS 55.0%
GCTAGACGGTCCGAAGAAGG+AGG - chr3_4:67191317-67191336 None:intergenic 60.0%
GCTGCTAGACGGTCCGAAGA+AGG - chr3_4:67191320-67191339 None:intergenic 60.0%
ACGGATCGCTGCACAGACTC+AGG - chr3_4:67191551-67191570 None:intergenic 60.0%
GAGTCTGTGCAGCGATCCGT+TGG + chr3_4:67191551-67191570 Msa0515820:CDS 60.0%
TCTGTGCAGCGATCCGTTGG+AGG + chr3_4:67191554-67191573 Msa0515820:CDS 60.0%
GCTAGACGGTCCGAAGAAGG+AGG - chr3_4:67191317-67191336 None:intergenic 60.0%
GCTGCTAGACGGTCCGAAGA+AGG - chr3_4:67191320-67191339 None:intergenic 60.0%
ACGGATCGCTGCACAGACTC+AGG - chr3_4:67191551-67191570 None:intergenic 60.0%
GAGTCTGTGCAGCGATCCGT+TGG + chr3_4:67191551-67191570 Msa0515820:CDS 60.0%
TCTGTGCAGCGATCCGTTGG+AGG + chr3_4:67191554-67191573 Msa0515820:CDS 60.0%
GGCGAGTCCCTCCACCATGT+AGG - chr3_4:67191240-67191259 None:intergenic 65.0%
TCCGGATTCAGCGAGGCGAG+CGG - chr3_4:67191261-67191280 None:intergenic 65.0%
GGCGAGTCCCTCCACCATGT+AGG - chr3_4:67191240-67191259 None:intergenic 65.0%
TCCGGATTCAGCGAGGCGAG+CGG - chr3_4:67191261-67191280 None:intergenic 65.0%
GCCGCTCGCCTCGCTGAATC+CGG + chr3_4:67191257-67191276 Msa0515820:CDS 70.0%
GCCGCTCGCCTCGCTGAATC+CGG + chr3_4:67191257-67191276 Msa0515820:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 67190528 67192225 67190528 ID=Msa0515820;Name=Msa0515820
chr3_4 mRNA 67190528 67192225 67190528 ID=Msa0515820-mRNA-1;Parent=Msa0515820;Name=Msa0515820-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|1|1|0|565
chr3_4 exon 67190528 67192225 67190528 ID=Msa0515820-mRNA-1:exon:17479;Parent=Msa0515820-mRNA-1
chr3_4 CDS 67190528 67192225 67190528 ID=Msa0515820-mRNA-1:cds;Parent=Msa0515820-mRNA-1
Gene Sequence

>Msa0515820

ATGTCATTAATTGGATCTTCTGAGCTAGCTCCACCATCACATAAAAGCACTAAGCTCAAATCGCCAAAGGAAATCGATGTTAACCCTGATTTTTCCTCCCAAATTTCTTATTCTCATAAGCATAGAAGCATGTATATCACAGATACCTACTCGAGCGAGAGTTACGAGAAGTACTTCAATGACTCACAGACAGAAGAACTAATAGAACCATCTAGTTCTGACATCTCTACAAGTTTCATGCCAACAAGGCAACGTGGTGCTGATCAGTCCAATTTCGGATCAGATTTGGTGATGGGAAATCGAAGCGTGAATTCAATTGAAGTTGATGGTGCAATGAGATCAAAACTAGAAGCATTGGAGAGGGCATTACTTGATGATAGTGACATTGAGGAAGAAGAGGAATATGGTAATTCTCAAAGCATGGAAATTGATCCTAACATTATTGATTGGGCTGATCCTGATCCAATTCAGAATATGCTGCTGTTCCATGACCATGACTCACCAAAGGAGTATTCGTCTCCAGATTCCAATATTAGCAGCATCAGTAGCACCACCAAAGAAATAACGAAAATTTCTAGGACCCCCAAGCAACTTCTTTATGAATGTGCTACTGCAATTTCAGAAGGAAATGAAGAGGAAGCATCGTCGATGATAAACAATCTCCGACGAATTGTCTCAATTCAAGGAGAGCCTTCCCAAAGAATTGCAGCCTACATGGTGGAGGGACTCGCCGCTCGCCTCGCTGAATCCGGAAAAAGTATTTATAGAGCTTTGAAATGCAAGGAACCTCCTTCTTCGGACCGTCTAGCAGCGATGCAGATACTGTTCGAGGTTTGTCCGTGTTTTAAATTCGGGTTTATTGCAGCTAACTATGCAATAACAGAAGCGGTTAAAGATGAAATGAAGATTCATATAATAGATTTTGATATCAATCAAGGAAGTCAGTACATAACCCTGATTCAAACACTTGCTTCGAGGTCTTGTAAGCCATCACATGTTAGATTGACCGGGATTGATGATCCTGAGTCTGTGCAGCGATCCGTTGGAGGACTAAAGATCATAGGACAAAGACTTGAACAACTAGCAGAAACACTTGGTTTGTCATTTGAATTTCGAGCGGTCTCATCGAGGACTTCAATTGTCACCTCATCGATGCTTGATTGTCGTCGTGGAGAAGCTCTTGTGGTGAACTTTGCATTCCAGCTTCATCACATGCCTGACGAGAGCGTTTCAACGGTGAACGAACGAGACCAACTTCTTCGCTTGGTAAAGAGTTTGAATCCAAAACTTGTAACGGTCGTGGAGCAAGATGTAAACACCAATACCGCCCCGTTTTTGCAGAGATTTGTTGAAGCTTACAATTACTACTCTGCTGTGTTTGAGTCACTTGATGTTACTCTTCCTAGAGAGAGCCAGGATAGGATGAATGTTGAGAGGCAATGCTTGGCAAGGGATATTGTTAATACGGTTGCATGTGAAGGTGAAGATAGGATAGAACGGTATGAAGTCGCGGGAAAATGGAGAGCTAGGATGACAATGGCTGGATTCAGTTCATCACCAATGAGCACAAATGTGAGTGATGAAATCCGAAAACAAGTTAAGTTGTACTGTGATAGGTACAAGATAAAGGAGGAAAAGGGTGCACTTCATTTTGGTTGGGAAGACAAAAACTTGATTGTTGCTTCAGCTTGGAGGTGA
Protein sequence

>Msa0515820

MSLIGSSELAPPSHKSTKLKSPKEIDVNPDFSSQISYSHKHRSMYITDTYSSESYEKYFNDSQTEELIEPSSSDISTSFMPTRQRGADQSNFGSDLVMGNRSVNSIEVDGAMRSKLEALERALLDDSDIEEEEEYGNSQSMEIDPNIIDWADPDPIQNMLLFHDHDSPKEYSSPDSNISSISSTTKEITKISRTPKQLLYECATAISEGNEEEASSMINNLRRIVSIQGEPSQRIAAYMVEGLAARLAESGKSIYRALKCKEPPSSDRLAAMQILFEVCPCFKFGFIAANYAITEAVKDEMKIHIIDFDINQGSQYITLIQTLASRSCKPSHVRLTGIDDPESVQRSVGGLKIIGQRLEQLAETLGLSFEFRAVSSRTSIVTSSMLDCRRGEALVVNFAFQLHHMPDESVSTVNERDQLLRLVKSLNPKLVTVVEQDVNTNTAPFLQRFVEAYNYYSAVFESLDVTLPRESQDRMNVERQCLARDIVNTVACEGEDRIERYEVAGKWRARMTMAGFSSSPMSTNVSDEIRKQVKLYCDRYKIKEEKGALHFGWEDKNLIVASAWR*