AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0479450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0479450 MtrunA17_Chr3g0142591 96.774 217 4 1 1 217 1 214 1.16e-152 422
Msa0479450 MtrunA17_Chr5g0405371 45.946 222 102 10 1 216 1 210 2.68e-46 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0479450 AT1G75720.2 43.519 216 83 8 8 210 1 190 2.87e-36 126
Msa0479450 AT1G75720.1 43.981 216 84 9 8 210 1 192 7.58e-36 125
Msa0479450 AT3G51220.1 35.096 208 100 7 15 212 4 186 4.21e-27 102
Msa0479450 AT2G17940.1 33.163 196 120 4 16 205 13 203 6.03e-23 92.4
Msa0479450 AT1G75720.3 43.791 153 57 6 71 210 12 148 1.74e-20 84.3

Find 65 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGTCTATCTCTGTATTTC+TGG 0.104808 3_3:+84479020 None:intergenic
CATAATGGAGCAAATGAAAA+TGG 0.193439 3_3:-84478025 Msa0479450:CDS
GAGAGAAGAATGAGATAATT+AGG 0.252145 3_3:+84479088 None:intergenic
TAAAGAAGAAAGCATGTTAA+TGG 0.275611 3_3:-84477930 Msa0479450:CDS
TCATTCATCATGGAACTTCT+TGG 0.313646 3_3:+84477366 None:intergenic
TCATTCATCATGGAACTTCT+TGG 0.313646 3_3:+84477546 None:intergenic
ACCCGGTTAGGTAATTATTC+TGG 0.316380 3_3:+84477292 None:intergenic
ACCCGGTTAGGTAATTATTC+TGG 0.316380 3_3:+84477472 None:intergenic
TGGAAAGGCACCCTTCTCTT+AGG 0.324014 3_3:-84477634 Msa0479450:CDS
AAAGAGGCTGTTACATTATT+CGG 0.326753 3_3:-84478976 Msa0479450:CDS
TAGACACAAGGGCGCCATTC+AGG 0.343210 3_3:-84479005 Msa0479450:CDS
GAGACAATTGGCATAATATA+TGG 0.345266 3_3:+84479150 None:intergenic
TACCAGAATAATTACCTAAC+CGG 0.350792 3_3:-84477294 Msa0479450:three_prime_UTR
TACCAGAATAATTACCTAAC+CGG 0.350792 3_3:-84477474 Msa0479450:three_prime_UTR
TTAGTAATGAACAATATTAA+AGG 0.352027 3_3:+84479128 None:intergenic
TCGTGCGTTCGAGTTCTTCC+TGG 0.369083 3_3:+84477888 None:intergenic
GGTGGATATGCTATTGGTCT+TGG 0.382415 3_3:+84479259 None:intergenic
AAGTCATTAATTCCACTTAT+CGG 0.388786 3_3:-84477599 Msa0479450:CDS
ATAATGGAGCAAATGAAAAT+GGG 0.406872 3_3:-84478024 Msa0479450:CDS
TTCTTCTTCTTCCTAAGAGA+AGG 0.423924 3_3:+84477623 None:intergenic
AGCATATCCACCCACCGCTT+TGG 0.425781 3_3:-84479248 Msa0479450:five_prime_UTR
CTTGAATACAAAACCCGGTT+AGG 0.438590 3_3:+84477280 None:intergenic
CTTGAATACAAAACCCGGTT+AGG 0.438590 3_3:+84477460 None:intergenic
GAAGAATGAGATAATTAGGT+TGG 0.441692 3_3:+84479092 None:intergenic
TGATGGAGTTTCAAAAGAAA+AGG 0.446231 3_3:-84477730 Msa0479450:CDS
TCATCAAGGTGTTGAGAAGT+TGG 0.449442 3_3:-84477654 Msa0479450:CDS
AGGTAACATGACATGAGAAA+TGG 0.454194 3_3:+84477682 None:intergenic
ATAATATATGGTATGAAGAT+TGG 0.459765 3_3:+84479162 None:intergenic
ATTTGATGAAGAATTAATGA+TGG 0.459950 3_3:-84477747 Msa0479450:CDS
TCTAATCTATAGATTCATAA+TGG 0.462589 3_3:-84478040 Msa0479450:intron
TCTTCTTCTTCCTAAGAGAA+GGG 0.487269 3_3:+84477624 None:intergenic
ACAATATTAAAGGAGACAAT+TGG 0.488865 3_3:+84479138 None:intergenic
ATGGGGTTGATCAGTCTTCA+AGG 0.494065 3_3:-84478006 Msa0479450:CDS
TAATGTAACAGCCTCTTTGA+CGG 0.499377 3_3:+84478981 None:intergenic
TGAAAACGTTACAGCTGAGC+TGG 0.505652 3_3:-84477981 Msa0479450:CDS
GCGGTGGGTGGATATGCTAT+TGG 0.509078 3_3:+84479253 None:intergenic
ACATTGTATGTCATCTCTCC+AGG 0.522311 3_3:-84477906 Msa0479450:CDS
TACAAACTCATCATTCATCA+TGG 0.529625 3_3:+84477356 None:intergenic
TACAAACTCATCATTCATCA+TGG 0.529625 3_3:+84477536 None:intergenic
ACAGAATTGTTCTAGTGAAG+TGG 0.530563 3_3:-84479204 Msa0479450:five_prime_UTR
AAAGATGAATAAGAAGAAAG+AGG 0.535423 3_3:-84479058 Msa0479450:exon
GCCTCTTTGACGGACCTGAA+TGG 0.535948 3_3:+84478991 None:intergenic
AAGATGAATAAGAAGAAAGA+GGG 0.553271 3_3:-84479057 Msa0479450:CDS
ACCAGAATAATTACCTAACC+GGG 0.556561 3_3:-84477293 Msa0479450:three_prime_UTR
ACCAGAATAATTACCTAACC+GGG 0.556561 3_3:-84477473 Msa0479450:three_prime_UTR
GCCATTCAGGTCCGTCAAAG+AGG 0.580083 3_3:-84478992 Msa0479450:CDS
ATGTTACCTCAAGGTCATCA+AGG 0.584789 3_3:-84477668 Msa0479450:CDS
TCTCATGTCATGTTACCTCA+AGG 0.592396 3_3:-84477677 Msa0479450:CDS
TGGGGTTGATCAGTCTTCAA+GGG 0.595004 3_3:-84478005 Msa0479450:CDS
AAGAGAGCCAAAGCGGTGGG+TGG 0.599926 3_3:+84479241 None:intergenic
TCAACACCTTGATGACCTTG+AGG 0.616815 3_3:+84477662 None:intergenic
GAGAAAGAGAGCCAAAGCGG+TGG 0.623143 3_3:+84479237 None:intergenic
AGAAAGAGAGCCAAAGCGGT+GGG 0.625444 3_3:+84479238 None:intergenic
AAGGTGTTGAGAAGTTGGAA+AGG 0.636189 3_3:-84477649 Msa0479450:CDS
TAATGGAGCAAATGAAAATG+GGG 0.636381 3_3:-84478023 Msa0479450:CDS
TCATTAATTCCACTTATCGG+AGG 0.648652 3_3:-84477596 Msa0479450:CDS
TGCAACTAAACTCAAACACC+AGG 0.653524 3_3:-84478920 Msa0479450:intron
AGAAACTTGAATACAAAACC+CGG 0.657488 3_3:+84477275 None:intergenic
AGAAACTTGAATACAAAACC+CGG 0.657488 3_3:+84477455 None:intergenic
AAACGTTACAGCTGAGCTGG+AGG 0.666569 3_3:-84477978 Msa0479450:CDS
ATGACATGAGAAATGGACGG+TGG 0.709324 3_3:+84477689 None:intergenic
TCTGAGAAAGAGAGCCAAAG+CGG 0.717444 3_3:+84479234 None:intergenic
GAAATACAGAGATAGACACA+AGG 0.731685 3_3:-84479017 Msa0479450:CDS
AAATACAGAGATAGACACAA+GGG 0.731958 3_3:-84479016 Msa0479450:CDS
AACATGACATGAGAAATGGA+CGG 0.745302 3_3:+84477686 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATATAATCATCTAA+TGG + chr3_3:84477852-84477871 None:intergenic 10.0%
!! AATAAATTATTAATAATGTG+TGG + chr3_3:84478425-84478444 None:intergenic 10.0%
!! ATAAAAATATAATCATCTAA+TGG + chr3_3:84477852-84477871 None:intergenic 10.0%
!! AATAAATTATTAATAATGTG+TGG + chr3_3:84478425-84478444 None:intergenic 10.0%
!! TTAGTAATGAACAATATTAA+AGG + chr3_3:84477379-84477398 None:intergenic 15.0%
!!! ATTTTATTAGAGTATCAAAA+TGG - chr3_3:84477631-84477650 Msa0479450:CDS 15.0%
!! ATTATAATATGTTTGTAAGT+GGG - chr3_3:84477766-84477785 Msa0479450:CDS 15.0%
!! TTAGTAATGAACAATATTAA+AGG + chr3_3:84477379-84477398 None:intergenic 15.0%
!!! ATTTTATTAGAGTATCAAAA+TGG - chr3_3:84477631-84477650 Msa0479450:CDS 15.0%
!! ATTATAATATGTTTGTAAGT+GGG - chr3_3:84477766-84477785 Msa0479450:CDS 15.0%
!! ATAATATATGGTATGAAGAT+TGG + chr3_3:84477345-84477364 None:intergenic 20.0%
!! AATAAAATTGAATGATGTGA+TGG + chr3_3:84477619-84477638 None:intergenic 20.0%
!! TTTAAATCACCAATTAAACT+TGG - chr3_3:84477675-84477694 Msa0479450:CDS 20.0%
!! CATTATAATATGTTTGTAAG+TGG - chr3_3:84477765-84477784 Msa0479450:CDS 20.0%
!! TTATAATATGTTTGTAAGTG+GGG - chr3_3:84477767-84477786 Msa0479450:CDS 20.0%
!!! ATCATCTAATGGATAAAATT+TGG + chr3_3:84477841-84477860 None:intergenic 20.0%
!!! TTCAATATAGTCTTACAATA+TGG - chr3_3:84478157-84478176 Msa0479450:intron 20.0%
!! TACTCAAATTCTTAATCATT+TGG - chr3_3:84478200-84478219 Msa0479450:intron 20.0%
!! AAATTTAATTATTGATCCGT+AGG - chr3_3:84478227-84478246 Msa0479450:intron 20.0%
!!! TTTTACTTTTTACATACCTA+CGG + chr3_3:84478246-84478265 None:intergenic 20.0%
!!! TAAGATGCACAAAATAATTT+TGG + chr3_3:84478324-84478343 None:intergenic 20.0%
!! TTAGAGAATAGAGATTAATA+AGG - chr3_3:84478341-84478360 Msa0479450:intron 20.0%
!! TCTAATCTATAGATTCATAA+TGG - chr3_3:84478464-84478483 Msa0479450:intron 20.0%
!! ATTTGATGAAGAATTAATGA+TGG - chr3_3:84478757-84478776 Msa0479450:intron 20.0%
!! ATAATATATGGTATGAAGAT+TGG + chr3_3:84477345-84477364 None:intergenic 20.0%
!! AATAAAATTGAATGATGTGA+TGG + chr3_3:84477619-84477638 None:intergenic 20.0%
!! TTTAAATCACCAATTAAACT+TGG - chr3_3:84477675-84477694 Msa0479450:CDS 20.0%
!! CATTATAATATGTTTGTAAG+TGG - chr3_3:84477765-84477784 Msa0479450:CDS 20.0%
!! TTATAATATGTTTGTAAGTG+GGG - chr3_3:84477767-84477786 Msa0479450:CDS 20.0%
!!! ATCATCTAATGGATAAAATT+TGG + chr3_3:84477841-84477860 None:intergenic 20.0%
!!! TTCAATATAGTCTTACAATA+TGG - chr3_3:84478157-84478176 Msa0479450:intron 20.0%
!! TACTCAAATTCTTAATCATT+TGG - chr3_3:84478200-84478219 Msa0479450:intron 20.0%
!! AAATTTAATTATTGATCCGT+AGG - chr3_3:84478227-84478246 Msa0479450:intron 20.0%
!!! TTTTACTTTTTACATACCTA+CGG + chr3_3:84478246-84478265 None:intergenic 20.0%
!!! TAAGATGCACAAAATAATTT+TGG + chr3_3:84478324-84478343 None:intergenic 20.0%
!! TTAGAGAATAGAGATTAATA+AGG - chr3_3:84478341-84478360 Msa0479450:intron 20.0%
!! TCTAATCTATAGATTCATAA+TGG - chr3_3:84478464-84478483 Msa0479450:intron 20.0%
!! ATTTGATGAAGAATTAATGA+TGG - chr3_3:84478757-84478776 Msa0479450:intron 20.0%
! ACAATATTAAAGGAGACAAT+TGG + chr3_3:84477369-84477388 None:intergenic 25.0%
! AAAGATGAATAAGAAGAAAG+AGG - chr3_3:84477446-84477465 Msa0479450:three_prime_UTR 25.0%
! AAGATGAATAAGAAGAAAGA+GGG - chr3_3:84477447-84477466 Msa0479450:three_prime_UTR 25.0%
!! ATTAGAGTATCAAAATGGTT+AGG - chr3_3:84477636-84477655 Msa0479450:CDS 25.0%
! GTAACTCAATCTTACAAAAT+CGG + chr3_3:84477811-84477830 None:intergenic 25.0%
! TTAAAAAAATATTGGCCAAG+AGG - chr3_3:84477879-84477898 Msa0479450:CDS 25.0%
! GTTTCAAAATAACATAGGAT+AGG + chr3_3:84477950-84477969 None:intergenic 25.0%
!!! ATCCTATGTTATTTTGAAAC+AGG - chr3_3:84477950-84477969 Msa0479450:CDS 25.0%
!!! TCCTATGTTATTTTGAAACA+GGG - chr3_3:84477951-84477970 Msa0479450:CDS 25.0%
! ACTTCCTTTATTCAATTAGA+CGG + chr3_3:84478058-84478077 None:intergenic 25.0%
! TTACTGTGTAAGATTAGTAT+CGG - chr3_3:84478274-84478293 Msa0479450:intron 25.0%
! AATCTATAGATTAGAAGACA+TGG + chr3_3:84478459-84478478 None:intergenic 25.0%
!! ATAATGGAGCAAATGAAAAT+GGG - chr3_3:84478480-84478499 Msa0479450:intron 25.0%
!! TAAAGAAGAAAGCATGTTAA+TGG - chr3_3:84478574-84478593 Msa0479450:intron 25.0%
! AAGTCATTAATTCCACTTAT+CGG - chr3_3:84478905-84478924 Msa0479450:intron 25.0%
!!! AGGTATTTTTTCTAGGAAAA+AGG - chr3_3:84478928-84478947 Msa0479450:CDS 25.0%
!!! GGTATTTTTTCTAGGAAAAA+GGG - chr3_3:84478929-84478948 Msa0479450:CDS 25.0%
!!! GTATTTTTGTTTTGTTTTCG+TGG - chr3_3:84479088-84479107 Msa0479450:five_prime_UTR 25.0%
!!! TGGTATTTTTTCTAGGAAAA+AGG - chr3_3:84479108-84479127 Msa0479450:five_prime_UTR 25.0%
!!! GGTATTTTTTCTAGGAAAAA+GGG - chr3_3:84478929-84478948 Msa0479450:CDS 25.0%
! ACAATATTAAAGGAGACAAT+TGG + chr3_3:84477369-84477388 None:intergenic 25.0%
! AAAGATGAATAAGAAGAAAG+AGG - chr3_3:84477446-84477465 Msa0479450:three_prime_UTR 25.0%
! AAGATGAATAAGAAGAAAGA+GGG - chr3_3:84477447-84477466 Msa0479450:three_prime_UTR 25.0%
!! ATTAGAGTATCAAAATGGTT+AGG - chr3_3:84477636-84477655 Msa0479450:CDS 25.0%
! GTAACTCAATCTTACAAAAT+CGG + chr3_3:84477811-84477830 None:intergenic 25.0%
! TTAAAAAAATATTGGCCAAG+AGG - chr3_3:84477879-84477898 Msa0479450:CDS 25.0%
! GTTTCAAAATAACATAGGAT+AGG + chr3_3:84477950-84477969 None:intergenic 25.0%
!!! ATCCTATGTTATTTTGAAAC+AGG - chr3_3:84477950-84477969 Msa0479450:CDS 25.0%
!!! TCCTATGTTATTTTGAAACA+GGG - chr3_3:84477951-84477970 Msa0479450:CDS 25.0%
! ACTTCCTTTATTCAATTAGA+CGG + chr3_3:84478058-84478077 None:intergenic 25.0%
! TTACTGTGTAAGATTAGTAT+CGG - chr3_3:84478274-84478293 Msa0479450:intron 25.0%
! AATCTATAGATTAGAAGACA+TGG + chr3_3:84478459-84478478 None:intergenic 25.0%
!! ATAATGGAGCAAATGAAAAT+GGG - chr3_3:84478480-84478499 Msa0479450:intron 25.0%
!! TAAAGAAGAAAGCATGTTAA+TGG - chr3_3:84478574-84478593 Msa0479450:intron 25.0%
! AAGTCATTAATTCCACTTAT+CGG - chr3_3:84478905-84478924 Msa0479450:intron 25.0%
!!! AGGTATTTTTTCTAGGAAAA+AGG - chr3_3:84478928-84478947 Msa0479450:CDS 25.0%
!!! GGTATTTTTTCTAGGAAAAA+GGG - chr3_3:84478929-84478948 Msa0479450:CDS 25.0%
!!! GTATTTTTGTTTTGTTTTCG+TGG - chr3_3:84479088-84479107 Msa0479450:five_prime_UTR 25.0%
!!! TGGTATTTTTTCTAGGAAAA+AGG - chr3_3:84479108-84479127 Msa0479450:five_prime_UTR 25.0%
!!! GGTATTTTTTCTAGGAAAAA+GGG - chr3_3:84478929-84478948 Msa0479450:CDS 25.0%
GAGACAATTGGCATAATATA+TGG + chr3_3:84477357-84477376 None:intergenic 30.0%
GAAGAATGAGATAATTAGGT+TGG + chr3_3:84477415-84477434 None:intergenic 30.0%
GAGAGAAGAATGAGATAATT+AGG + chr3_3:84477419-84477438 None:intergenic 30.0%
AAATACAGAGATAGACACAA+GGG - chr3_3:84477488-84477507 Msa0479450:three_prime_UTR 30.0%
AAAGAGGCTGTTACATTATT+CGG - chr3_3:84477528-84477547 Msa0479450:three_prime_UTR 30.0%
!! TCTTACAAAATCGGTTTGTA+AGG + chr3_3:84477802-84477821 None:intergenic 30.0%
ACCCTGTTTCAAAATAACAT+AGG + chr3_3:84477955-84477974 None:intergenic 30.0%
TTCACCGTCTAATTGAATAA+AGG - chr3_3:84478051-84478070 Msa0479450:intron 30.0%
! CATAATGGAGCAAATGAAAA+TGG - chr3_3:84478479-84478498 Msa0479450:intron 30.0%
TAATGGAGCAAATGAAAATG+GGG - chr3_3:84478481-84478500 Msa0479450:intron 30.0%
CGAAACTGAAAAAAATCAAG+TGG - chr3_3:84478667-84478686 Msa0479450:intron 30.0%
! TCTTGAACTTTTCACTTCAA+AGG + chr3_3:84478738-84478757 None:intergenic 30.0%
TGATGGAGTTTCAAAAGAAA+AGG - chr3_3:84478774-84478793 Msa0479450:intron 30.0%
!!! TTATCGGAGGTATTTTTTCT+AGG - chr3_3:84478921-84478940 Msa0479450:CDS 30.0%
TACAAACTCATCATTCATCA+TGG + chr3_3:84478971-84478990 None:intergenic 30.0%
TACCAGAATAATTACCTAAC+CGG - chr3_3:84479030-84479049 Msa0479450:CDS 30.0%
AGAAACTTGAATACAAAACC+CGG + chr3_3:84479052-84479071 None:intergenic 30.0%
!!! GTTTTCGTGGTATTTTTTCT+AGG - chr3_3:84479101-84479120 Msa0479450:five_prime_UTR 30.0%
TACAAACTCATCATTCATCA+TGG + chr3_3:84478971-84478990 None:intergenic 30.0%
TACCAGAATAATTACCTAAC+CGG - chr3_3:84479030-84479049 Msa0479450:CDS 30.0%
AGAAACTTGAATACAAAACC+CGG + chr3_3:84479052-84479071 None:intergenic 30.0%
GAGACAATTGGCATAATATA+TGG + chr3_3:84477357-84477376 None:intergenic 30.0%
GAAGAATGAGATAATTAGGT+TGG + chr3_3:84477415-84477434 None:intergenic 30.0%
GAGAGAAGAATGAGATAATT+AGG + chr3_3:84477419-84477438 None:intergenic 30.0%
AAATACAGAGATAGACACAA+GGG - chr3_3:84477488-84477507 Msa0479450:three_prime_UTR 30.0%
AAAGAGGCTGTTACATTATT+CGG - chr3_3:84477528-84477547 Msa0479450:three_prime_UTR 30.0%
!! TCTTACAAAATCGGTTTGTA+AGG + chr3_3:84477802-84477821 None:intergenic 30.0%
ACCCTGTTTCAAAATAACAT+AGG + chr3_3:84477955-84477974 None:intergenic 30.0%
TTCACCGTCTAATTGAATAA+AGG - chr3_3:84478051-84478070 Msa0479450:intron 30.0%
! CATAATGGAGCAAATGAAAA+TGG - chr3_3:84478479-84478498 Msa0479450:intron 30.0%
TAATGGAGCAAATGAAAATG+GGG - chr3_3:84478481-84478500 Msa0479450:intron 30.0%
CGAAACTGAAAAAAATCAAG+TGG - chr3_3:84478667-84478686 Msa0479450:intron 30.0%
! TCTTGAACTTTTCACTTCAA+AGG + chr3_3:84478738-84478757 None:intergenic 30.0%
TGATGGAGTTTCAAAAGAAA+AGG - chr3_3:84478774-84478793 Msa0479450:intron 30.0%
!!! TTATCGGAGGTATTTTTTCT+AGG - chr3_3:84478921-84478940 Msa0479450:CDS 30.0%
TACAAACTCATCATTCATCA+TGG + chr3_3:84478971-84478990 None:intergenic 30.0%
TACCAGAATAATTACCTAAC+CGG - chr3_3:84479030-84479049 Msa0479450:CDS 30.0%
AGAAACTTGAATACAAAACC+CGG + chr3_3:84479052-84479071 None:intergenic 30.0%
!!! GTTTTCGTGGTATTTTTTCT+AGG - chr3_3:84479101-84479120 Msa0479450:five_prime_UTR 30.0%
TACAAACTCATCATTCATCA+TGG + chr3_3:84478971-84478990 None:intergenic 30.0%
TACCAGAATAATTACCTAAC+CGG - chr3_3:84479030-84479049 Msa0479450:CDS 30.0%
AGAAACTTGAATACAAAACC+CGG + chr3_3:84479052-84479071 None:intergenic 30.0%
ACAGAATTGTTCTAGTGAAG+TGG - chr3_3:84477300-84477319 Msa0479450:three_prime_UTR 35.0%
TGTGTCTATCTCTGTATTTC+TGG + chr3_3:84477487-84477506 None:intergenic 35.0%
GAAATACAGAGATAGACACA+AGG - chr3_3:84477487-84477506 Msa0479450:three_prime_UTR 35.0%
TAATGTAACAGCCTCTTTGA+CGG + chr3_3:84477526-84477545 None:intergenic 35.0%
!! TTCGGTGATAAAGTTTTAGC+TGG - chr3_3:84477546-84477565 Msa0479450:exon 35.0%
GTGACACATCCAAGTTTAAT+TGG + chr3_3:84477687-84477706 None:intergenic 35.0%
AAATGTCCAAGAATTCCTCT+TGG + chr3_3:84477897-84477916 None:intergenic 35.0%
AGGAGAATCTTATTGATGGA+TGG + chr3_3:84477930-84477949 None:intergenic 35.0%
GGATAGGAGAATCTTATTGA+TGG + chr3_3:84477934-84477953 None:intergenic 35.0%
TCCTCATCATCACTTGTTTA+TGG - chr3_3:84478383-84478402 Msa0479450:intron 35.0%
! ACCATAAACAAGTGATGATG+AGG + chr3_3:84478387-84478406 None:intergenic 35.0%
AACATGACATGAGAAATGGA+CGG + chr3_3:84478821-84478840 None:intergenic 35.0%
AGGTAACATGACATGAGAAA+TGG + chr3_3:84478825-84478844 None:intergenic 35.0%
TCTTCTTCTTCCTAAGAGAA+GGG + chr3_3:84478883-84478902 None:intergenic 35.0%
TTCTTCTTCTTCCTAAGAGA+AGG + chr3_3:84478884-84478903 None:intergenic 35.0%
TCATTAATTCCACTTATCGG+AGG - chr3_3:84478908-84478927 Msa0479450:intron 35.0%
GAAAAAATACCTCCGATAAG+TGG + chr3_3:84478920-84478939 None:intergenic 35.0%
TCATTCATCATGGAACTTCT+TGG + chr3_3:84478961-84478980 None:intergenic 35.0%
ACCAGAATAATTACCTAACC+GGG - chr3_3:84479031-84479050 Msa0479450:CDS 35.0%
TCATTCATCATGGAACTTCT+TGG + chr3_3:84478961-84478980 None:intergenic 35.0%
ACCAGAATAATTACCTAACC+GGG - chr3_3:84479031-84479050 Msa0479450:CDS 35.0%
ACAGAATTGTTCTAGTGAAG+TGG - chr3_3:84477300-84477319 Msa0479450:three_prime_UTR 35.0%
TGTGTCTATCTCTGTATTTC+TGG + chr3_3:84477487-84477506 None:intergenic 35.0%
GAAATACAGAGATAGACACA+AGG - chr3_3:84477487-84477506 Msa0479450:three_prime_UTR 35.0%
TAATGTAACAGCCTCTTTGA+CGG + chr3_3:84477526-84477545 None:intergenic 35.0%
!! TTCGGTGATAAAGTTTTAGC+TGG - chr3_3:84477546-84477565 Msa0479450:exon 35.0%
GTGACACATCCAAGTTTAAT+TGG + chr3_3:84477687-84477706 None:intergenic 35.0%
AAATGTCCAAGAATTCCTCT+TGG + chr3_3:84477897-84477916 None:intergenic 35.0%
AGGAGAATCTTATTGATGGA+TGG + chr3_3:84477930-84477949 None:intergenic 35.0%
GGATAGGAGAATCTTATTGA+TGG + chr3_3:84477934-84477953 None:intergenic 35.0%
TCCTCATCATCACTTGTTTA+TGG - chr3_3:84478383-84478402 Msa0479450:intron 35.0%
! ACCATAAACAAGTGATGATG+AGG + chr3_3:84478387-84478406 None:intergenic 35.0%
AACATGACATGAGAAATGGA+CGG + chr3_3:84478821-84478840 None:intergenic 35.0%
AGGTAACATGACATGAGAAA+TGG + chr3_3:84478825-84478844 None:intergenic 35.0%
TCTTCTTCTTCCTAAGAGAA+GGG + chr3_3:84478883-84478902 None:intergenic 35.0%
TTCTTCTTCTTCCTAAGAGA+AGG + chr3_3:84478884-84478903 None:intergenic 35.0%
TCATTAATTCCACTTATCGG+AGG - chr3_3:84478908-84478927 Msa0479450:intron 35.0%
GAAAAAATACCTCCGATAAG+TGG + chr3_3:84478920-84478939 None:intergenic 35.0%
TCATTCATCATGGAACTTCT+TGG + chr3_3:84478961-84478980 None:intergenic 35.0%
ACCAGAATAATTACCTAACC+GGG - chr3_3:84479031-84479050 Msa0479450:CDS 35.0%
TCATTCATCATGGAACTTCT+TGG + chr3_3:84478961-84478980 None:intergenic 35.0%
ACCAGAATAATTACCTAACC+GGG - chr3_3:84479031-84479050 Msa0479450:CDS 35.0%
TGCAACTAAACTCAAACACC+AGG - chr3_3:84477584-84477603 Msa0479450:CDS 40.0%
! ATGTGATGGTGTATAGAACC+TGG + chr3_3:84477605-84477624 None:intergenic 40.0%
TGTCGTATATCATAGAACGC+AGG + chr3_3:84477747-84477766 None:intergenic 40.0%
TATTGGCCAAGAGGAATTCT+TGG - chr3_3:84477888-84477907 Msa0479450:CDS 40.0%
ACATTGTATGTCATCTCTCC+AGG - chr3_3:84478598-84478617 Msa0479450:intron 40.0%
TCTCATGTCATGTTACCTCA+AGG - chr3_3:84478827-84478846 Msa0479450:intron 40.0%
ATGTTACCTCAAGGTCATCA+AGG - chr3_3:84478836-84478855 Msa0479450:intron 40.0%
!! TCATCAAGGTGTTGAGAAGT+TGG - chr3_3:84478850-84478869 Msa0479450:intron 40.0%
!! AAGGTGTTGAGAAGTTGGAA+AGG - chr3_3:84478855-84478874 Msa0479450:intron 40.0%
ACCCGGTTAGGTAATTATTC+TGG + chr3_3:84479035-84479054 None:intergenic 40.0%
CTTGAATACAAAACCCGGTT+AGG + chr3_3:84479047-84479066 None:intergenic 40.0%
ACCCGGTTAGGTAATTATTC+TGG + chr3_3:84479035-84479054 None:intergenic 40.0%
CTTGAATACAAAACCCGGTT+AGG + chr3_3:84479047-84479066 None:intergenic 40.0%
TGCAACTAAACTCAAACACC+AGG - chr3_3:84477584-84477603 Msa0479450:CDS 40.0%
! ATGTGATGGTGTATAGAACC+TGG + chr3_3:84477605-84477624 None:intergenic 40.0%
TGTCGTATATCATAGAACGC+AGG + chr3_3:84477747-84477766 None:intergenic 40.0%
TATTGGCCAAGAGGAATTCT+TGG - chr3_3:84477888-84477907 Msa0479450:CDS 40.0%
ACATTGTATGTCATCTCTCC+AGG - chr3_3:84478598-84478617 Msa0479450:intron 40.0%
TCTCATGTCATGTTACCTCA+AGG - chr3_3:84478827-84478846 Msa0479450:intron 40.0%
ATGTTACCTCAAGGTCATCA+AGG - chr3_3:84478836-84478855 Msa0479450:intron 40.0%
!! TCATCAAGGTGTTGAGAAGT+TGG - chr3_3:84478850-84478869 Msa0479450:intron 40.0%
!! AAGGTGTTGAGAAGTTGGAA+AGG - chr3_3:84478855-84478874 Msa0479450:intron 40.0%
ACCCGGTTAGGTAATTATTC+TGG + chr3_3:84479035-84479054 None:intergenic 40.0%
CTTGAATACAAAACCCGGTT+AGG + chr3_3:84479047-84479066 None:intergenic 40.0%
ACCCGGTTAGGTAATTATTC+TGG + chr3_3:84479035-84479054 None:intergenic 40.0%
CTTGAATACAAAACCCGGTT+AGG + chr3_3:84479047-84479066 None:intergenic 40.0%
! GGTGGATATGCTATTGGTCT+TGG + chr3_3:84477248-84477267 None:intergenic 45.0%
TCTGAGAAAGAGAGCCAAAG+CGG + chr3_3:84477273-84477292 None:intergenic 45.0%
!! CAAAATCGGTTTGTAAGGCG+AGG + chr3_3:84477797-84477816 None:intergenic 45.0%
! ATGGGGTTGATCAGTCTTCA+AGG - chr3_3:84478498-84478517 Msa0479450:intron 45.0%
! TGGGGTTGATCAGTCTTCAA+GGG - chr3_3:84478499-84478518 Msa0479450:intron 45.0%
TGAAAACGTTACAGCTGAGC+TGG - chr3_3:84478523-84478542 Msa0479450:intron 45.0%
ATGACATGAGAAATGGACGG+TGG + chr3_3:84478818-84478837 None:intergenic 45.0%
! TCAACACCTTGATGACCTTG+AGG + chr3_3:84478845-84478864 None:intergenic 45.0%
! GGTGGATATGCTATTGGTCT+TGG + chr3_3:84477248-84477267 None:intergenic 45.0%
TCTGAGAAAGAGAGCCAAAG+CGG + chr3_3:84477273-84477292 None:intergenic 45.0%
!! CAAAATCGGTTTGTAAGGCG+AGG + chr3_3:84477797-84477816 None:intergenic 45.0%
! ATGGGGTTGATCAGTCTTCA+AGG - chr3_3:84478498-84478517 Msa0479450:intron 45.0%
! TGGGGTTGATCAGTCTTCAA+GGG - chr3_3:84478499-84478518 Msa0479450:intron 45.0%
TGAAAACGTTACAGCTGAGC+TGG - chr3_3:84478523-84478542 Msa0479450:intron 45.0%
ATGACATGAGAAATGGACGG+TGG + chr3_3:84478818-84478837 None:intergenic 45.0%
! TCAACACCTTGATGACCTTG+AGG + chr3_3:84478845-84478864 None:intergenic 45.0%
!! TATACTTATTAAAAAAATAT+TGG - chr3_3:84477871-84477890 Msa0479450:CDS 5.0%
!! TATACTTATTAAAAAAATAT+TGG - chr3_3:84477871-84477890 Msa0479450:CDS 5.0%
AGAAAGAGAGCCAAAGCGGT+GGG + chr3_3:84477269-84477288 None:intergenic 50.0%
AAACGTTACAGCTGAGCTGG+AGG - chr3_3:84478526-84478545 Msa0479450:intron 50.0%
!! TGGAAAGGCACCCTTCTCTT+AGG - chr3_3:84478870-84478889 Msa0479450:intron 50.0%
AGAAAGAGAGCCAAAGCGGT+GGG + chr3_3:84477269-84477288 None:intergenic 50.0%
AAACGTTACAGCTGAGCTGG+AGG - chr3_3:84478526-84478545 Msa0479450:intron 50.0%
!! TGGAAAGGCACCCTTCTCTT+AGG - chr3_3:84478870-84478889 Msa0479450:intron 50.0%
GCGGTGGGTGGATATGCTAT+TGG + chr3_3:84477254-84477273 None:intergenic 55.0%
AGCATATCCACCCACCGCTT+TGG - chr3_3:84477256-84477275 Msa0479450:three_prime_UTR 55.0%
GAGAAAGAGAGCCAAAGCGG+TGG + chr3_3:84477270-84477289 None:intergenic 55.0%
!! TAGACACAAGGGCGCCATTC+AGG - chr3_3:84477499-84477518 Msa0479450:three_prime_UTR 55.0%
GCCATTCAGGTCCGTCAAAG+AGG - chr3_3:84477512-84477531 Msa0479450:three_prime_UTR 55.0%
! GCCTCTTTGACGGACCTGAA+TGG + chr3_3:84477516-84477535 None:intergenic 55.0%
! TCGTGCGTTCGAGTTCTTCC+TGG + chr3_3:84478619-84478638 None:intergenic 55.0%
GCGGTGGGTGGATATGCTAT+TGG + chr3_3:84477254-84477273 None:intergenic 55.0%
AGCATATCCACCCACCGCTT+TGG - chr3_3:84477256-84477275 Msa0479450:three_prime_UTR 55.0%
GAGAAAGAGAGCCAAAGCGG+TGG + chr3_3:84477270-84477289 None:intergenic 55.0%
!! TAGACACAAGGGCGCCATTC+AGG - chr3_3:84477499-84477518 Msa0479450:three_prime_UTR 55.0%
GCCATTCAGGTCCGTCAAAG+AGG - chr3_3:84477512-84477531 Msa0479450:three_prime_UTR 55.0%
! GCCTCTTTGACGGACCTGAA+TGG + chr3_3:84477516-84477535 None:intergenic 55.0%
! TCGTGCGTTCGAGTTCTTCC+TGG + chr3_3:84478619-84478638 None:intergenic 55.0%
AAGAGAGCCAAAGCGGTGGG+TGG + chr3_3:84477266-84477285 None:intergenic 60.0%
AAGAGAGCCAAAGCGGTGGG+TGG + chr3_3:84477266-84477285 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 84477238 84479288 84477238 ID=Msa0479450;Name=Msa0479450
chr3_3 mRNA 84477238 84479288 84477238 ID=Msa0479450-mRNA-1;Parent=Msa0479450;Name=Msa0479450-mRNA-1;_AED=0.00;_eAED=0.00;_QI=212|1|1|1|1|1|2|315|217
chr3_3 exon 84477238 84478050 84477238 ID=Msa0479450-mRNA-1:exon:23957;Parent=Msa0479450-mRNA-1
chr3_3 exon 84478921 84479288 84478921 ID=Msa0479450-mRNA-1:exon:23956;Parent=Msa0479450-mRNA-1
chr3_3 five_prime_UTR 84479077 84479288 84479077 ID=Msa0479450-mRNA-1:five_prime_utr;Parent=Msa0479450-mRNA-1
chr3_3 CDS 84478921 84479076 84478921 ID=Msa0479450-mRNA-1:cds;Parent=Msa0479450-mRNA-1
chr3_3 CDS 84477553 84478050 84477553 ID=Msa0479450-mRNA-1:cds;Parent=Msa0479450-mRNA-1
chr3_3 three_prime_UTR 84477238 84477552 84477238 ID=Msa0479450-mRNA-1:three_prime_utr;Parent=Msa0479450-mRNA-1
Gene Sequence

>Msa0479450

ATGAATAAGAAGAAAGAGGGTGTGATGCTGCTCACCAGAAATACAGAGATAGACACAAGGGCGCCATTCAGGTCCGTCAAAGAGGCTGTTACATTATTCGGTGATAAAGTTTTAGCTGGAGAGCTCTATGCAACTGCAACTAAACTCAAACACCAGATTCATAATGGAGCAAATGAAAATGGGGTTGATCAGTCTTCAAGGGTTGAAAACGTTACAGCTGAGCTGGAGGAGACACGAGAGAATCTAGAGAAAGCTAAAGAAGAAAGCATGTTAATGGCACATTGTATGTCATCTCTCCAGGAAGAACTCGAACGCACGAAGCAAGAGCTCGAACAATTGAAGCAACGCGAAACTGAAAAAAATCAAGTGGAATCATCAGAAACTGAAGATGTGAAGTTTGTTGAGAATTTAACATCCTTTGAAGTGAAAAGTTCAAGATTTGATGAAGAATTAATGATGGAGTTTCAAAAGAAAAGGTATGTGACGTTCGCGAATCCACCGTCCATTTCTCATGTCATGTTACCTCAAGGTCATCAAGGTGTTGAGAAGTTGGAAAGGCACCCTTCTCTTAGGAAGAAGAAGAAGAAGTCATTAATTCCACTTATCGGAGGTATTTTTTCTAGGAAAAAGGGAACTAGCCAAGAAGTTCCATGA
Protein sequence

>Msa0479450

MNKKKEGVMLLTRNTEIDTRAPFRSVKEAVTLFGDKVLAGELYATATKLKHQIHNGANENGVDQSSRVENVTAELEETRENLEKAKEESMLMAHCMSSLQEELERTKQELEQLKQRETEKNQVESSETEDVKFVENLTSFEVKSSRFDEELMMEFQKKRYVTFANPPSISHVMLPQGHQGVEKLERHPSLRKKKKKSLIPLIGGIFSRKKGTSQEVP*