AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0435550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0435550 MtrunA17_Chr3g0142591 97.235 217 3 1 1 217 1 214 4.70e-153 423
Msa0435550 MtrunA17_Chr5g0405371 45.946 222 102 10 1 216 1 210 3.11e-46 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0435550 AT1G75720.2 43.519 216 83 8 8 210 1 190 3.34e-36 126
Msa0435550 AT1G75720.1 43.981 216 84 9 8 210 1 192 9.30e-36 125
Msa0435550 AT3G51220.1 34.951 206 103 6 15 212 4 186 4.12e-27 102
Msa0435550 AT2G17940.1 33.673 196 119 4 16 205 13 203 2.06e-23 93.6
Msa0435550 AT1G75720.3 43.791 153 57 6 71 210 12 148 2.13e-20 84.3

Find 59 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGTCTATCTCTGTATTTC+TGG 0.104808 3_2:+88735327 None:intergenic
CATAATGGAGCAAATGAAAA+TGG 0.193439 3_2:-88734335 Msa0435550:CDS
GAGAGAAGAATGAGATAATT+AGG 0.261473 3_2:+88735403 None:intergenic
TAAAGAAGAAAGCATGTTAA+TGG 0.275611 3_2:-88734240 Msa0435550:CDS
TCATTCATCATGGAACTTCT+TGG 0.313646 3_2:+88733856 None:intergenic
ACCCGGTTAGGTAATTATTC+TGG 0.316380 3_2:+88733782 None:intergenic
TGGAAAGGCACCCTTCTCTT+AGG 0.324014 3_2:-88733944 Msa0435550:CDS
AAAGAGGCTGTTACATTATT+CGG 0.326753 3_2:-88735283 Msa0435550:CDS
TAGACACAAGGGCGCCATTC+AGG 0.343210 3_2:-88735312 Msa0435550:CDS
GAGACAATTGGCATAATATA+TGG 0.345266 3_2:+88735465 None:intergenic
TACCAGAATAATTACCTAAC+CGG 0.350792 3_2:-88733784 Msa0435550:three_prime_UTR
TTAGTAATGAACAATATTAA+AGG 0.352027 3_2:+88735443 None:intergenic
TCGTGCGTTCGAGTTCTTCC+TGG 0.369083 3_2:+88734198 None:intergenic
AGTACAGCAGTAGCAGAGTA+TGG 0.387211 3_2:-88733635 Msa0435550:three_prime_UTR
AAGTCATTAATTCCACTTAT+CGG 0.388786 3_2:-88733909 Msa0435550:CDS
ATAATGGAGCAAATGAAAAT+GGG 0.406872 3_2:-88734334 Msa0435550:CDS
TTCTTCTTCTTCCTAAGAGA+AGG 0.423924 3_2:+88733933 None:intergenic
AGCATATCCACCCACCGCTT+TGG 0.425781 3_2:-88735563 Msa0435550:five_prime_UTR
CTTGAATACAAAACCCGGTT+AGG 0.438590 3_2:+88733770 None:intergenic
GAAGAATGAGATAATTAGGT+TGG 0.441692 3_2:+88735407 None:intergenic
TGATGGAGTTTCAAAAGAAA+AGG 0.446231 3_2:-88734040 Msa0435550:CDS
TCATCAAGGTGTTGAGAAGT+TGG 0.449442 3_2:-88733964 Msa0435550:CDS
GGTGGATATGCTATTTGTCT+TGG 0.454026 3_2:+88735574 None:intergenic
AGGTAACATGACATGAGAAA+TGG 0.454194 3_2:+88733992 None:intergenic
ATAATATATGGTATGAAGAT+TGG 0.459765 3_2:+88735477 None:intergenic
ATTTGATGAAGAATTAATGA+TGG 0.459950 3_2:-88734057 Msa0435550:CDS
TCTAATCTATAGATTCATAA+TGG 0.462589 3_2:-88734350 Msa0435550:intron
TCTTCTTCTTCCTAAGAGAA+GGG 0.487269 3_2:+88733934 None:intergenic
ACAATATTAAAGGAGACAAT+TGG 0.488865 3_2:+88735453 None:intergenic
ATGGGGTTGATCAGTCTTCA+AGG 0.494065 3_2:-88734316 Msa0435550:CDS
TAATGTAACAGCCTCTTTGA+CGG 0.499377 3_2:+88735288 None:intergenic
TGAAAACGTTACAGCTGAGC+TGG 0.505652 3_2:-88734291 Msa0435550:CDS
ACATTGTATGTCATCTCTCC+AGG 0.522311 3_2:-88734216 Msa0435550:CDS
TACAAACTCATCATTCATCA+TGG 0.529625 3_2:+88733846 None:intergenic
ACAGAATTGTTCTAGTGAAG+TGG 0.530563 3_2:-88735519 Msa0435550:five_prime_UTR
AAAGATGAATAAGAAGAAAG+AGG 0.535423 3_2:-88735365 Msa0435550:exon
GCCTCTTTGACGGACCTGAA+TGG 0.535948 3_2:+88735298 None:intergenic
AAGATGAATAAGAAGAAAGA+GGG 0.553271 3_2:-88735364 Msa0435550:CDS
ACCAGAATAATTACCTAACC+GGG 0.556561 3_2:-88733783 Msa0435550:three_prime_UTR
GCCATTCAGGTCCGTCAAAG+AGG 0.580083 3_2:-88735299 Msa0435550:CDS
ATGTTACCTCAAGGTCATCA+AGG 0.584789 3_2:-88733978 Msa0435550:CDS
TCTCATGTCATGTTACCTCA+AGG 0.592396 3_2:-88733987 Msa0435550:CDS
TGGGGTTGATCAGTCTTCAA+GGG 0.595004 3_2:-88734315 Msa0435550:CDS
CAGAAATAGAGCCAAAGCGG+TGG 0.595909 3_2:+88735552 None:intergenic
AATAGAGCCAAAGCGGTGGG+TGG 0.603252 3_2:+88735556 None:intergenic
GTATGGATACTTGCATATTG+TGG 0.609698 3_2:-88733618 Msa0435550:three_prime_UTR
TCAACACCTTGATGACCTTG+AGG 0.616815 3_2:+88733972 None:intergenic
AGAAATAGAGCCAAAGCGGT+GGG 0.632622 3_2:+88735553 None:intergenic
AAGGTGTTGAGAAGTTGGAA+AGG 0.636189 3_2:-88733959 Msa0435550:CDS
TAATGGAGCAAATGAAAATG+GGG 0.636381 3_2:-88734333 Msa0435550:CDS
TCATTAATTCCACTTATCGG+AGG 0.648652 3_2:-88733906 Msa0435550:CDS
TGCAACTAAACTCAAACACC+AGG 0.653524 3_2:-88735227 Msa0435550:intron
AGAAACTTGAATACAAAACC+CGG 0.657488 3_2:+88733765 None:intergenic
AAACGTTACAGCTGAGCTGG+AGG 0.666569 3_2:-88734288 Msa0435550:CDS
ATGACATGAGAAATGGACGG+TGG 0.709324 3_2:+88733999 None:intergenic
TCTCAGAAATAGAGCCAAAG+CGG 0.715352 3_2:+88735549 None:intergenic
GAAATACAGAGATAGACACA+AGG 0.731685 3_2:-88735324 Msa0435550:CDS
AAATACAGAGATAGACACAA+GGG 0.731958 3_2:-88735323 Msa0435550:CDS
AACATGACATGAGAAATGGA+CGG 0.745302 3_2:+88733996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATTATTAATAATGTG+TGG + chr3_2:88734788-88734807 None:intergenic 10.0%
!! AATAAATTATTAATAATGTG+TGG + chr3_2:88734788-88734807 None:intergenic 10.0%
!! TTAGTAATGAACAATATTAA+AGG + chr3_2:88733737-88733756 None:intergenic 15.0%
!!! ATTTTATTAGAGTATCAAAA+TGG - chr3_2:88733997-88734016 Msa0435550:CDS 15.0%
!! ATTATAATATGTTTGTAAGT+GGG - chr3_2:88734132-88734151 Msa0435550:CDS 15.0%
!! TATAAAATATAACCATCTAA+TGG + chr3_2:88734218-88734237 None:intergenic 15.0%
!! TTAGTAATGAACAATATTAA+AGG + chr3_2:88733737-88733756 None:intergenic 15.0%
!!! ATTTTATTAGAGTATCAAAA+TGG - chr3_2:88733997-88734016 Msa0435550:CDS 15.0%
!! ATTATAATATGTTTGTAAGT+GGG - chr3_2:88734132-88734151 Msa0435550:CDS 15.0%
!! TATAAAATATAACCATCTAA+TGG + chr3_2:88734218-88734237 None:intergenic 15.0%
!! ATAATATATGGTATGAAGAT+TGG + chr3_2:88733703-88733722 None:intergenic 20.0%
!! AATAAAATTGAATGATGTGA+TGG + chr3_2:88733985-88734004 None:intergenic 20.0%
!! TTTAAATCACCAATTAAACT+TGG - chr3_2:88734041-88734060 Msa0435550:CDS 20.0%
!! CATTATAATATGTTTGTAAG+TGG - chr3_2:88734131-88734150 Msa0435550:CDS 20.0%
!! TTATAATATGTTTGTAAGTG+GGG - chr3_2:88734133-88734152 Msa0435550:CDS 20.0%
!! TACTCAAATTCTTAATCATT+TGG - chr3_2:88734563-88734582 Msa0435550:intron 20.0%
!! AAATTTAATTATTGATCCGT+AGG - chr3_2:88734590-88734609 Msa0435550:intron 20.0%
!!! TTTTACTTTTTACATACCTA+CGG + chr3_2:88734609-88734628 None:intergenic 20.0%
!!! TAAGATGCACAAAATAATTT+TGG + chr3_2:88734687-88734706 None:intergenic 20.0%
!! TTAGAGAATAGAGATTAATA+AGG - chr3_2:88734704-88734723 Msa0435550:intron 20.0%
!! TCTAATCTATAGATTCATAA+TGG - chr3_2:88734827-88734846 Msa0435550:intron 20.0%
!! ATTTGATGAAGAATTAATGA+TGG - chr3_2:88735120-88735139 Msa0435550:intron 20.0%
!! ATAATATATGGTATGAAGAT+TGG + chr3_2:88733703-88733722 None:intergenic 20.0%
!! AATAAAATTGAATGATGTGA+TGG + chr3_2:88733985-88734004 None:intergenic 20.0%
!! TTTAAATCACCAATTAAACT+TGG - chr3_2:88734041-88734060 Msa0435550:CDS 20.0%
!! CATTATAATATGTTTGTAAG+TGG - chr3_2:88734131-88734150 Msa0435550:CDS 20.0%
!! TTATAATATGTTTGTAAGTG+GGG - chr3_2:88734133-88734152 Msa0435550:CDS 20.0%
!! TACTCAAATTCTTAATCATT+TGG - chr3_2:88734563-88734582 Msa0435550:intron 20.0%
!! AAATTTAATTATTGATCCGT+AGG - chr3_2:88734590-88734609 Msa0435550:intron 20.0%
!!! TTTTACTTTTTACATACCTA+CGG + chr3_2:88734609-88734628 None:intergenic 20.0%
!!! TAAGATGCACAAAATAATTT+TGG + chr3_2:88734687-88734706 None:intergenic 20.0%
!! TTAGAGAATAGAGATTAATA+AGG - chr3_2:88734704-88734723 Msa0435550:intron 20.0%
!! TCTAATCTATAGATTCATAA+TGG - chr3_2:88734827-88734846 Msa0435550:intron 20.0%
!! ATTTGATGAAGAATTAATGA+TGG - chr3_2:88735120-88735139 Msa0435550:intron 20.0%
! ACAATATTAAAGGAGACAAT+TGG + chr3_2:88733727-88733746 None:intergenic 25.0%
! AAAGATGAATAAGAAGAAAG+AGG - chr3_2:88733812-88733831 Msa0435550:three_prime_UTR 25.0%
! AAGATGAATAAGAAGAAAGA+GGG - chr3_2:88733813-88733832 Msa0435550:three_prime_UTR 25.0%
!! ATTAGAGTATCAAAATGGTT+AGG - chr3_2:88734002-88734021 Msa0435550:CDS 25.0%
! GTAACTCAATCTTACAAAAT+CGG + chr3_2:88734177-88734196 None:intergenic 25.0%
! TCCAAATATTATCCATTAGA+TGG - chr3_2:88734203-88734222 Msa0435550:CDS 25.0%
!! ACCATCTAATGGATAATATT+TGG + chr3_2:88734207-88734226 None:intergenic 25.0%
! TTAAAAAAATAATGGCCAAG+AGG - chr3_2:88734244-88734263 Msa0435550:CDS 25.0%
! ACTTCCTTTATTCAATTAGA+CGG + chr3_2:88734423-88734442 None:intergenic 25.0%
!! TTCAATGTAGTCTTACAATA+TGG - chr3_2:88734520-88734539 Msa0435550:intron 25.0%
! TTACTGTGTAAGATTAGTAT+CGG - chr3_2:88734637-88734656 Msa0435550:intron 25.0%
!! ATAATGGAGCAAATGAAAAT+GGG - chr3_2:88734843-88734862 Msa0435550:intron 25.0%
!! TAAAGAAGAAAGCATGTTAA+TGG - chr3_2:88734937-88734956 Msa0435550:intron 25.0%
! AAGTCATTAATTCCACTTAT+CGG - chr3_2:88735268-88735287 Msa0435550:CDS 25.0%
!!! AGGTATTTTTTCTAGGAAAA+AGG - chr3_2:88735291-88735310 Msa0435550:CDS 25.0%
!!! GGTATTTTTTCTAGGAAAAA+GGG - chr3_2:88735292-88735311 Msa0435550:CDS 25.0%
! ACAATATTAAAGGAGACAAT+TGG + chr3_2:88733727-88733746 None:intergenic 25.0%
! AAAGATGAATAAGAAGAAAG+AGG - chr3_2:88733812-88733831 Msa0435550:three_prime_UTR 25.0%
! AAGATGAATAAGAAGAAAGA+GGG - chr3_2:88733813-88733832 Msa0435550:three_prime_UTR 25.0%
!! ATTAGAGTATCAAAATGGTT+AGG - chr3_2:88734002-88734021 Msa0435550:CDS 25.0%
! GTAACTCAATCTTACAAAAT+CGG + chr3_2:88734177-88734196 None:intergenic 25.0%
! TCCAAATATTATCCATTAGA+TGG - chr3_2:88734203-88734222 Msa0435550:CDS 25.0%
!! ACCATCTAATGGATAATATT+TGG + chr3_2:88734207-88734226 None:intergenic 25.0%
! TTAAAAAAATAATGGCCAAG+AGG - chr3_2:88734244-88734263 Msa0435550:CDS 25.0%
! ACTTCCTTTATTCAATTAGA+CGG + chr3_2:88734423-88734442 None:intergenic 25.0%
!! TTCAATGTAGTCTTACAATA+TGG - chr3_2:88734520-88734539 Msa0435550:intron 25.0%
! TTACTGTGTAAGATTAGTAT+CGG - chr3_2:88734637-88734656 Msa0435550:intron 25.0%
!! ATAATGGAGCAAATGAAAAT+GGG - chr3_2:88734843-88734862 Msa0435550:intron 25.0%
!! TAAAGAAGAAAGCATGTTAA+TGG - chr3_2:88734937-88734956 Msa0435550:intron 25.0%
! AAGTCATTAATTCCACTTAT+CGG - chr3_2:88735268-88735287 Msa0435550:CDS 25.0%
!!! AGGTATTTTTTCTAGGAAAA+AGG - chr3_2:88735291-88735310 Msa0435550:CDS 25.0%
!!! GGTATTTTTTCTAGGAAAAA+GGG - chr3_2:88735292-88735311 Msa0435550:CDS 25.0%
GAGACAATTGGCATAATATA+TGG + chr3_2:88733715-88733734 None:intergenic 30.0%
GAAGAATGAGATAATTAGGT+TGG + chr3_2:88733773-88733792 None:intergenic 30.0%
GAGAGAAGAATGAGATAATT+AGG + chr3_2:88733777-88733796 None:intergenic 30.0%
AAATACAGAGATAGACACAA+GGG - chr3_2:88733854-88733873 Msa0435550:exon 30.0%
AAAGAGGCTGTTACATTATT+CGG - chr3_2:88733894-88733913 Msa0435550:CDS 30.0%
!! TCTTACAAAATCGGTTTGTA+AGG + chr3_2:88734168-88734187 None:intergenic 30.0%
GTTTCAAAGTAACATAGGAT+AGG + chr3_2:88734315-88734334 None:intergenic 30.0%
!! ATCCTATGTTACTTTGAAAC+AGG - chr3_2:88734315-88734334 Msa0435550:CDS 30.0%
!! TCCTATGTTACTTTGAAACA+GGG - chr3_2:88734316-88734335 Msa0435550:CDS 30.0%
TTCACCGTCTAATTGAATAA+AGG - chr3_2:88734416-88734435 Msa0435550:intron 30.0%
! CATAATGGAGCAAATGAAAA+TGG - chr3_2:88734842-88734861 Msa0435550:intron 30.0%
TAATGGAGCAAATGAAAATG+GGG - chr3_2:88734844-88734863 Msa0435550:intron 30.0%
CGAAACTGAAAAAAATCAAG+TGG - chr3_2:88735030-88735049 Msa0435550:intron 30.0%
! TCTTGAACTTTTCACTTCAA+AGG + chr3_2:88735101-88735120 None:intergenic 30.0%
TGATGGAGTTTCAAAAGAAA+AGG - chr3_2:88735137-88735156 Msa0435550:intron 30.0%
!!! TTATCGGAGGTATTTTTTCT+AGG - chr3_2:88735284-88735303 Msa0435550:CDS 30.0%
TACAAACTCATCATTCATCA+TGG + chr3_2:88735334-88735353 None:intergenic 30.0%
TACCAGAATAATTACCTAAC+CGG - chr3_2:88735393-88735412 Msa0435550:five_prime_UTR 30.0%
AGAAACTTGAATACAAAACC+CGG + chr3_2:88735415-88735434 None:intergenic 30.0%
GAGACAATTGGCATAATATA+TGG + chr3_2:88733715-88733734 None:intergenic 30.0%
GAAGAATGAGATAATTAGGT+TGG + chr3_2:88733773-88733792 None:intergenic 30.0%
GAGAGAAGAATGAGATAATT+AGG + chr3_2:88733777-88733796 None:intergenic 30.0%
AAATACAGAGATAGACACAA+GGG - chr3_2:88733854-88733873 Msa0435550:exon 30.0%
AAAGAGGCTGTTACATTATT+CGG - chr3_2:88733894-88733913 Msa0435550:CDS 30.0%
!! TCTTACAAAATCGGTTTGTA+AGG + chr3_2:88734168-88734187 None:intergenic 30.0%
GTTTCAAAGTAACATAGGAT+AGG + chr3_2:88734315-88734334 None:intergenic 30.0%
!! ATCCTATGTTACTTTGAAAC+AGG - chr3_2:88734315-88734334 Msa0435550:CDS 30.0%
!! TCCTATGTTACTTTGAAACA+GGG - chr3_2:88734316-88734335 Msa0435550:CDS 30.0%
TTCACCGTCTAATTGAATAA+AGG - chr3_2:88734416-88734435 Msa0435550:intron 30.0%
! CATAATGGAGCAAATGAAAA+TGG - chr3_2:88734842-88734861 Msa0435550:intron 30.0%
TAATGGAGCAAATGAAAATG+GGG - chr3_2:88734844-88734863 Msa0435550:intron 30.0%
CGAAACTGAAAAAAATCAAG+TGG - chr3_2:88735030-88735049 Msa0435550:intron 30.0%
! TCTTGAACTTTTCACTTCAA+AGG + chr3_2:88735101-88735120 None:intergenic 30.0%
TGATGGAGTTTCAAAAGAAA+AGG - chr3_2:88735137-88735156 Msa0435550:intron 30.0%
!!! TTATCGGAGGTATTTTTTCT+AGG - chr3_2:88735284-88735303 Msa0435550:CDS 30.0%
TACAAACTCATCATTCATCA+TGG + chr3_2:88735334-88735353 None:intergenic 30.0%
TACCAGAATAATTACCTAAC+CGG - chr3_2:88735393-88735412 Msa0435550:five_prime_UTR 30.0%
AGAAACTTGAATACAAAACC+CGG + chr3_2:88735415-88735434 None:intergenic 30.0%
ACAGAATTGTTCTAGTGAAG+TGG - chr3_2:88733658-88733677 Msa0435550:three_prime_UTR 35.0%
TGTGTCTATCTCTGTATTTC+TGG + chr3_2:88733853-88733872 None:intergenic 35.0%
GAAATACAGAGATAGACACA+AGG - chr3_2:88733853-88733872 Msa0435550:exon 35.0%
TAATGTAACAGCCTCTTTGA+CGG + chr3_2:88733892-88733911 None:intergenic 35.0%
!! TTCGGTGATAAAGTTTTAGC+TGG - chr3_2:88733912-88733931 Msa0435550:CDS 35.0%
GTGACACATCCAAGTTTAAT+TGG + chr3_2:88734053-88734072 None:intergenic 35.0%
AAATGTCCAAGAATTCCTCT+TGG + chr3_2:88734262-88734281 None:intergenic 35.0%
AGGAGAATCTTATTGATGGA+TGG + chr3_2:88734295-88734314 None:intergenic 35.0%
GGATAGGAGAATCTTATTGA+TGG + chr3_2:88734299-88734318 None:intergenic 35.0%
ACCCTGTTTCAAAGTAACAT+AGG + chr3_2:88734320-88734339 None:intergenic 35.0%
TCCTCATCATCACTTGTTTA+TGG - chr3_2:88734746-88734765 Msa0435550:intron 35.0%
! ACCATAAACAAGTGATGATG+AGG + chr3_2:88734750-88734769 None:intergenic 35.0%
AACATGACATGAGAAATGGA+CGG + chr3_2:88735184-88735203 None:intergenic 35.0%
AGGTAACATGACATGAGAAA+TGG + chr3_2:88735188-88735207 None:intergenic 35.0%
TCTTCTTCTTCCTAAGAGAA+GGG + chr3_2:88735246-88735265 None:intergenic 35.0%
TTCTTCTTCTTCCTAAGAGA+AGG + chr3_2:88735247-88735266 None:intergenic 35.0%
TCATTAATTCCACTTATCGG+AGG - chr3_2:88735271-88735290 Msa0435550:CDS 35.0%
GAAAAAATACCTCCGATAAG+TGG + chr3_2:88735283-88735302 None:intergenic 35.0%
TCATTCATCATGGAACTTCT+TGG + chr3_2:88735324-88735343 None:intergenic 35.0%
ACCAGAATAATTACCTAACC+GGG - chr3_2:88735394-88735413 Msa0435550:five_prime_UTR 35.0%
GTATGGATACTTGCATATTG+TGG - chr3_2:88735559-88735578 Msa0435550:five_prime_UTR 35.0%
ACAGAATTGTTCTAGTGAAG+TGG - chr3_2:88733658-88733677 Msa0435550:three_prime_UTR 35.0%
TGTGTCTATCTCTGTATTTC+TGG + chr3_2:88733853-88733872 None:intergenic 35.0%
GAAATACAGAGATAGACACA+AGG - chr3_2:88733853-88733872 Msa0435550:exon 35.0%
TAATGTAACAGCCTCTTTGA+CGG + chr3_2:88733892-88733911 None:intergenic 35.0%
!! TTCGGTGATAAAGTTTTAGC+TGG - chr3_2:88733912-88733931 Msa0435550:CDS 35.0%
GTGACACATCCAAGTTTAAT+TGG + chr3_2:88734053-88734072 None:intergenic 35.0%
AAATGTCCAAGAATTCCTCT+TGG + chr3_2:88734262-88734281 None:intergenic 35.0%
AGGAGAATCTTATTGATGGA+TGG + chr3_2:88734295-88734314 None:intergenic 35.0%
GGATAGGAGAATCTTATTGA+TGG + chr3_2:88734299-88734318 None:intergenic 35.0%
ACCCTGTTTCAAAGTAACAT+AGG + chr3_2:88734320-88734339 None:intergenic 35.0%
TCCTCATCATCACTTGTTTA+TGG - chr3_2:88734746-88734765 Msa0435550:intron 35.0%
! ACCATAAACAAGTGATGATG+AGG + chr3_2:88734750-88734769 None:intergenic 35.0%
AACATGACATGAGAAATGGA+CGG + chr3_2:88735184-88735203 None:intergenic 35.0%
AGGTAACATGACATGAGAAA+TGG + chr3_2:88735188-88735207 None:intergenic 35.0%
TCTTCTTCTTCCTAAGAGAA+GGG + chr3_2:88735246-88735265 None:intergenic 35.0%
TTCTTCTTCTTCCTAAGAGA+AGG + chr3_2:88735247-88735266 None:intergenic 35.0%
TCATTAATTCCACTTATCGG+AGG - chr3_2:88735271-88735290 Msa0435550:CDS 35.0%
GAAAAAATACCTCCGATAAG+TGG + chr3_2:88735283-88735302 None:intergenic 35.0%
TCATTCATCATGGAACTTCT+TGG + chr3_2:88735324-88735343 None:intergenic 35.0%
ACCAGAATAATTACCTAACC+GGG - chr3_2:88735394-88735413 Msa0435550:five_prime_UTR 35.0%
GTATGGATACTTGCATATTG+TGG - chr3_2:88735559-88735578 Msa0435550:five_prime_UTR 35.0%
! GGTGGATATGCTATTTGTCT+TGG + chr3_2:88733606-88733625 None:intergenic 40.0%
TCTCAGAAATAGAGCCAAAG+CGG + chr3_2:88733631-88733650 None:intergenic 40.0%
TGCAACTAAACTCAAACACC+AGG - chr3_2:88733950-88733969 Msa0435550:CDS 40.0%
! ATGTGATGGTGTATAGAACC+TGG + chr3_2:88733971-88733990 None:intergenic 40.0%
TGTCGTATATCACAGAACGT+AGG + chr3_2:88734113-88734132 None:intergenic 40.0%
TAATGGCCAAGAGGAATTCT+TGG - chr3_2:88734253-88734272 Msa0435550:CDS 40.0%
ACATTGTATGTCATCTCTCC+AGG - chr3_2:88734961-88734980 Msa0435550:intron 40.0%
TCTCATGTCATGTTACCTCA+AGG - chr3_2:88735190-88735209 Msa0435550:intron 40.0%
ATGTTACCTCAAGGTCATCA+AGG - chr3_2:88735199-88735218 Msa0435550:intron 40.0%
!! TCATCAAGGTGTTGAGAAGT+TGG - chr3_2:88735213-88735232 Msa0435550:intron 40.0%
!! AAGGTGTTGAGAAGTTGGAA+AGG - chr3_2:88735218-88735237 Msa0435550:intron 40.0%
ACCCGGTTAGGTAATTATTC+TGG + chr3_2:88735398-88735417 None:intergenic 40.0%
CTTGAATACAAAACCCGGTT+AGG + chr3_2:88735410-88735429 None:intergenic 40.0%
! GGTGGATATGCTATTTGTCT+TGG + chr3_2:88733606-88733625 None:intergenic 40.0%
TCTCAGAAATAGAGCCAAAG+CGG + chr3_2:88733631-88733650 None:intergenic 40.0%
TGCAACTAAACTCAAACACC+AGG - chr3_2:88733950-88733969 Msa0435550:CDS 40.0%
! ATGTGATGGTGTATAGAACC+TGG + chr3_2:88733971-88733990 None:intergenic 40.0%
TGTCGTATATCACAGAACGT+AGG + chr3_2:88734113-88734132 None:intergenic 40.0%
TAATGGCCAAGAGGAATTCT+TGG - chr3_2:88734253-88734272 Msa0435550:CDS 40.0%
ACATTGTATGTCATCTCTCC+AGG - chr3_2:88734961-88734980 Msa0435550:intron 40.0%
TCTCATGTCATGTTACCTCA+AGG - chr3_2:88735190-88735209 Msa0435550:intron 40.0%
ATGTTACCTCAAGGTCATCA+AGG - chr3_2:88735199-88735218 Msa0435550:intron 40.0%
!! TCATCAAGGTGTTGAGAAGT+TGG - chr3_2:88735213-88735232 Msa0435550:intron 40.0%
!! AAGGTGTTGAGAAGTTGGAA+AGG - chr3_2:88735218-88735237 Msa0435550:intron 40.0%
ACCCGGTTAGGTAATTATTC+TGG + chr3_2:88735398-88735417 None:intergenic 40.0%
CTTGAATACAAAACCCGGTT+AGG + chr3_2:88735410-88735429 None:intergenic 40.0%
AGAAATAGAGCCAAAGCGGT+GGG + chr3_2:88733627-88733646 None:intergenic 45.0%
!! CAAAATCGGTTTGTAAGGCG+AGG + chr3_2:88734163-88734182 None:intergenic 45.0%
! ATGGGGTTGATCAGTCTTCA+AGG - chr3_2:88734861-88734880 Msa0435550:intron 45.0%
! TGGGGTTGATCAGTCTTCAA+GGG - chr3_2:88734862-88734881 Msa0435550:intron 45.0%
TGAAAACGTTACAGCTGAGC+TGG - chr3_2:88734886-88734905 Msa0435550:intron 45.0%
ATGACATGAGAAATGGACGG+TGG + chr3_2:88735181-88735200 None:intergenic 45.0%
! TCAACACCTTGATGACCTTG+AGG + chr3_2:88735208-88735227 None:intergenic 45.0%
AGTACAGCAGTAGCAGAGTA+TGG - chr3_2:88735542-88735561 Msa0435550:five_prime_UTR 45.0%
AGAAATAGAGCCAAAGCGGT+GGG + chr3_2:88733627-88733646 None:intergenic 45.0%
!! CAAAATCGGTTTGTAAGGCG+AGG + chr3_2:88734163-88734182 None:intergenic 45.0%
! ATGGGGTTGATCAGTCTTCA+AGG - chr3_2:88734861-88734880 Msa0435550:intron 45.0%
! TGGGGTTGATCAGTCTTCAA+GGG - chr3_2:88734862-88734881 Msa0435550:intron 45.0%
TGAAAACGTTACAGCTGAGC+TGG - chr3_2:88734886-88734905 Msa0435550:intron 45.0%
ATGACATGAGAAATGGACGG+TGG + chr3_2:88735181-88735200 None:intergenic 45.0%
! TCAACACCTTGATGACCTTG+AGG + chr3_2:88735208-88735227 None:intergenic 45.0%
AGTACAGCAGTAGCAGAGTA+TGG - chr3_2:88735542-88735561 Msa0435550:five_prime_UTR 45.0%
!! TATACTTATTAAAAAAATAA+TGG - chr3_2:88734236-88734255 Msa0435550:CDS 5.0%
!! TATACTTATTAAAAAAATAA+TGG - chr3_2:88734236-88734255 Msa0435550:CDS 5.0%
CAGAAATAGAGCCAAAGCGG+TGG + chr3_2:88733628-88733647 None:intergenic 50.0%
AAACGTTACAGCTGAGCTGG+AGG - chr3_2:88734889-88734908 Msa0435550:intron 50.0%
!! TGGAAAGGCACCCTTCTCTT+AGG - chr3_2:88735233-88735252 Msa0435550:CDS 50.0%
CAGAAATAGAGCCAAAGCGG+TGG + chr3_2:88733628-88733647 None:intergenic 50.0%
AAACGTTACAGCTGAGCTGG+AGG - chr3_2:88734889-88734908 Msa0435550:intron 50.0%
!! TGGAAAGGCACCCTTCTCTT+AGG - chr3_2:88735233-88735252 Msa0435550:CDS 50.0%
AGCATATCCACCCACCGCTT+TGG - chr3_2:88733614-88733633 Msa0435550:three_prime_UTR 55.0%
AATAGAGCCAAAGCGGTGGG+TGG + chr3_2:88733624-88733643 None:intergenic 55.0%
!! TAGACACAAGGGCGCCATTC+AGG - chr3_2:88733865-88733884 Msa0435550:CDS 55.0%
GCCATTCAGGTCCGTCAAAG+AGG - chr3_2:88733878-88733897 Msa0435550:CDS 55.0%
! GCCTCTTTGACGGACCTGAA+TGG + chr3_2:88733882-88733901 None:intergenic 55.0%
! TCGTGCGTTCGAGTTCTTCC+TGG + chr3_2:88734982-88735001 None:intergenic 55.0%
AGCATATCCACCCACCGCTT+TGG - chr3_2:88733614-88733633 Msa0435550:three_prime_UTR 55.0%
AATAGAGCCAAAGCGGTGGG+TGG + chr3_2:88733624-88733643 None:intergenic 55.0%
!! TAGACACAAGGGCGCCATTC+AGG - chr3_2:88733865-88733884 Msa0435550:CDS 55.0%
GCCATTCAGGTCCGTCAAAG+AGG - chr3_2:88733878-88733897 Msa0435550:CDS 55.0%
! GCCTCTTTGACGGACCTGAA+TGG + chr3_2:88733882-88733901 None:intergenic 55.0%
! TCGTGCGTTCGAGTTCTTCC+TGG + chr3_2:88734982-88735001 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 88733596 88735603 88733596 ID=Msa0435550;Name=Msa0435550
chr3_2 mRNA 88733596 88735603 88733596 ID=Msa0435550-mRNA-1;Parent=Msa0435550;Name=Msa0435550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=220|1|1|1|1|1|2|267|217
chr3_2 exon 88733596 88734360 88733596 ID=Msa0435550-mRNA-1:exon:26196;Parent=Msa0435550-mRNA-1
chr3_2 exon 88735228 88735603 88735228 ID=Msa0435550-mRNA-1:exon:26195;Parent=Msa0435550-mRNA-1
chr3_2 five_prime_UTR 88735384 88735603 88735384 ID=Msa0435550-mRNA-1:five_prime_utr;Parent=Msa0435550-mRNA-1
chr3_2 CDS 88735228 88735383 88735228 ID=Msa0435550-mRNA-1:cds;Parent=Msa0435550-mRNA-1
chr3_2 CDS 88733863 88734360 88733863 ID=Msa0435550-mRNA-1:cds;Parent=Msa0435550-mRNA-1
chr3_2 three_prime_UTR 88733596 88733862 88733596 ID=Msa0435550-mRNA-1:three_prime_utr;Parent=Msa0435550-mRNA-1
Gene Sequence

>Msa0435550

ATGAATAAGAAGAAAGAGGGTGTGATGCTGCTCACCAGAAATACAGAGATAGACACAAGGGCGCCATTCAGGTCCGTCAAAGAGGCTGTTACATTATTCGGTGATAAAGTTTTAGCTGGAGAGCTCTATGCAACTGCAACTAAACTCAAACACCAGATTCATAATGGAGCAAATGAAAATGGGGTTGATCAGTCTTCAAGGGTTGAAAACGTTACAGCTGAGCTGGAGGAGACACGAGAGAATCTAGAGAAAGCTAAAGAAGAAAGCATGTTAATGGCACATTGTATGTCATCTCTCCAGGAAGAACTCGAACGCACGAAGCAAGAGCTCGAACAATTGAAGCAACGCGAAACTGAAAAAAATCAAGTGGAATCATCAGAAACTGAAGATGTGAAGTTTGTTGAGAATTTAACAACCTTTGAAGTGAAAAGTTCAAGATTTGATGAAGAATTAATGATGGAGTTTCAAAAGAAAAGGTATGTGACGTTCGCGAATCCACCGTCCATTTCTCATGTCATGTTACCTCAAGGTCATCAAGGTGTTGAGAAGTTGGAAAGGCACCCTTCTCTTAGGAAGAAGAAGAAGAAGTCATTAATTCCACTTATCGGAGGTATTTTTTCTAGGAAAAAGGGAACTAGCCAAGAAGTTCCATGA
Protein sequence

>Msa0435550

MNKKKEGVMLLTRNTEIDTRAPFRSVKEAVTLFGDKVLAGELYATATKLKHQIHNGANENGVDQSSRVENVTAELEETRENLEKAKEESMLMAHCMSSLQEELERTKQELEQLKQRETEKNQVESSETEDVKFVENLTTFEVKSSRFDEELMMEFQKKRYVTFANPPSISHVMLPQGHQGVEKLERHPSLRKKKKKSLIPLIGGIFSRKKGTSQEVP*