AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0489420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0489420 MtrunA17_Chr3g0079511 65.714 105 26 3 28 124 3 105 2.83e-39 130
Msa0489420 MtrunA17_Chr3g0079501 78.261 46 10 0 1 46 1 46 3.16e-20 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0489420 AT2G30695.2 40.000 105 54 3 28 124 42 145 2.27e-18 77.8
Msa0489420 AT2G30695.3 40.000 105 54 3 28 124 42 145 2.27e-18 77.8
Msa0489420 AT2G30695.1 40.000 105 54 3 28 124 42 145 2.27e-18 77.8
Msa0489420 AT2G30695.4 46.250 80 41 2 28 106 42 120 4.85e-16 70.1

Find 64 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATGATGTGTTTCAGAAAA+TGG 0.139014 3_4:+9762472 Msa0489420:CDS
ATCAGACTTGCTGGTGATTT+TGG 0.160107 3_4:-9762775 None:intergenic
TAACAGTTATGTTATCTTAC+AGG 0.237471 3_4:+9760182 Msa0489420:five_prime_UTR
TGAGAAACACAAATGTTCTT+TGG 0.265301 3_4:-9764859 None:intergenic
ACCTCTAATAAAATGTCTTT+TGG 0.275489 3_4:-9763310 None:intergenic
CCAGGATTTCGTAGAGTAAA+AGG 0.298327 3_4:+9762515 Msa0489420:CDS
ATGATTATTATCAGACTTGC+TGG 0.318683 3_4:-9762784 None:intergenic
CAGATAAGCATCGAGGTATC+TGG 0.342280 3_4:+9762428 Msa0489420:intron
ATGGAGTGACATTGCTTCTT+CGG 0.375429 3_4:+9764396 Msa0489420:three_prime_UTR
TTTATATGTTAAAATCTTAC+CGG 0.375682 3_4:+9764373 Msa0489420:three_prime_UTR
AAACTTCCGCACGTAATATC+TGG 0.383321 3_4:-9762717 None:intergenic
AATGTCAATCCTTTGATATA+AGG 0.393635 3_4:-9762871 None:intergenic
CTACAAAATGAATTTAACTT+TGG 0.404253 3_4:+9764754 Msa0489420:three_prime_UTR
TCTGTCTTTCTTTGAAGCAT+GGG 0.424747 3_4:+9760524 Msa0489420:CDS
TTCTGTCTTTCTTTGAAGCA+TGG 0.425898 3_4:+9760523 Msa0489420:CDS
TTGCTTAAATCAAGTGTTTG+AGG 0.431774 3_4:+9760247 Msa0489420:five_prime_UTR
CTGTGGTGTTCACCATCATT+AGG 0.438728 3_4:-9764571 None:intergenic
AGATCTTGAAACTACATTTG+AGG 0.477759 3_4:+9764291 Msa0489420:three_prime_UTR
GTGCGGTTATTATCATATAG+AGG 0.480784 3_4:+9760269 Msa0489420:five_prime_UTR
TGGAGTTAGATTTAAGATGA+AGG 0.495200 3_4:-9760356 None:intergenic
CTCAACTGTGGCTGAGTATG+TGG 0.495219 3_4:+9763381 Msa0489420:three_prime_UTR
AAAATCACGGTGGCATTTCG+CGG 0.495444 3_4:+9764498 Msa0489420:three_prime_UTR
CTTTCTTTGAAGCATGGGCA+CGG 0.495628 3_4:+9760529 Msa0489420:CDS
AGAAGCAATGTCACTCCATC+CGG 0.498714 3_4:-9764392 None:intergenic
TAGATATCATGAAAGGTACT+TGG 0.502334 3_4:+9762673 Msa0489420:three_prime_UTR
AGAACATTTGTGTTTCTCAA+AGG 0.503564 3_4:+9764863 Msa0489420:three_prime_UTR
ACATTGTTTCCTCCTAATGA+TGG 0.504607 3_4:+9764559 Msa0489420:three_prime_UTR
AGATATCATGAAAGGTACTT+GGG 0.518504 3_4:+9762674 Msa0489420:three_prime_UTR
GGACATCACTCTACAAACTC+TGG 0.519633 3_4:+9764707 Msa0489420:three_prime_UTR
ACAACACAGAATTTCAAATG+AGG 0.522251 3_4:-9760296 None:intergenic
AAGGAGGTGAAGTATTTATG+TGG 0.522765 3_4:+9762534 Msa0489420:CDS
GCTTCAAAAGTAGAATTGAC+AGG 0.524464 3_4:+9764348 Msa0489420:three_prime_UTR
CATCTTAAATCTAACTCCAC+AGG 0.526554 3_4:+9760360 Msa0489420:CDS
ATTTGGAGAAGAGAAATGCT+AGG 0.530452 3_4:-9764923 None:intergenic
TATGTTAAAATCTTACCGGA+TGG 0.533410 3_4:+9764377 Msa0489420:three_prime_UTR
TGATGATGTTAGAGAACTAA+AGG 0.538789 3_4:+9761562 Msa0489420:CDS
ACTCTACGAAATCCTGGGAT+CGG 0.550289 3_4:-9762509 None:intergenic
CTTGAAACTACATTTGAGGA+AGG 0.550589 3_4:+9764295 Msa0489420:three_prime_UTR
AATATCTGGTTAGTCAAACT+TGG 0.550831 3_4:-9762703 None:intergenic
AACAGTTATGTTATCTTACA+GGG 0.557820 3_4:+9760183 Msa0489420:five_prime_UTR
TGTCATCTGACTCCTCAAAA+CGG 0.574338 3_4:+9762822 Msa0489420:three_prime_UTR
TGCTTCTTCGGTCTGAACCA+AGG 0.577590 3_4:+9764408 Msa0489420:three_prime_UTR
AAAGCTGCACAACCGATCCC+AGG 0.580477 3_4:+9762497 Msa0489420:CDS
ATCTCTTGAAAGCAATGTAG+TGG 0.582364 3_4:+9764785 Msa0489420:three_prime_UTR
TTTGGAGAAGAGAAATGCTA+GGG 0.589324 3_4:-9764922 None:intergenic
TCTCTTAAAATGATTCAAAG+TGG 0.591059 3_4:+9764433 Msa0489420:three_prime_UTR
TACAAACTCTGGAGCAGCCG+CGG 0.613220 3_4:+9764718 Msa0489420:three_prime_UTR
CAGATTGATAGCCTTAGCAA+AGG 0.625867 3_4:+9762583 Msa0489420:CDS
TGTGGCTGAGTATGTGGAGA+AGG 0.628591 3_4:+9763387 Msa0489420:three_prime_UTR
TAAATCAAGTGTTTGAGGTG+CGG 0.632050 3_4:+9760252 Msa0489420:five_prime_UTR
GGAAATAATCAACTCAACTG+TGG 0.634122 3_4:+9763369 Msa0489420:three_prime_UTR
GACTAACCAGATATTACGTG+CGG 0.643491 3_4:+9762711 Msa0489420:three_prime_UTR
TGGTGTTCACCATCATTAGG+AGG 0.649027 3_4:-9764568 None:intergenic
GCTTGCAGATGATAAAACAC+AGG 0.649255 3_4:+9760455 Msa0489420:intron
GATATCATGAAAGGTACTTG+GGG 0.655458 3_4:+9762675 Msa0489420:three_prime_UTR
ATCAATGCGTGAGAAAACAT+AGG 0.655741 3_4:+9764686 Msa0489420:three_prime_UTR
TGTCATCTAAGTGAAAATCA+CGG 0.658079 3_4:+9764485 Msa0489420:three_prime_UTR
ACGTTAGTAGATATCATGAA+AGG 0.664067 3_4:+9762666 Msa0489420:three_prime_UTR
GTAGTTCTCAAAATCAACCG+CGG 0.706727 3_4:-9764735 None:intergenic
TTCTGTCCAGATAAGCATCG+AGG 0.718719 3_4:+9762421 Msa0489420:intron
GGATTTCGTAGAGTAAAAGG+AGG 0.718882 3_4:+9762518 Msa0489420:CDS
AGTTATGTTATCTTACAGGG+TGG 0.719636 3_4:+9760186 Msa0489420:five_prime_UTR
CATCTAAGTGAAAATCACGG+TGG 0.807475 3_4:+9764488 Msa0489420:three_prime_UTR
TATCTGGAAACAGAACACAG+AGG 0.877907 3_4:+9762444 Msa0489420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTATTTTTTTAATTAT+AGG + chr3_4:9763927-9763946 Msa0489420:intron 0.0%
!!! TATTATTATCATTTTCTATT+TGG - chr3_4:9762752-9762771 None:intergenic 10.0%
!! AACTATAAAAACATAAAAAT+AGG - chr3_4:9763005-9763024 None:intergenic 10.0%
!! TAATTTCAACTTAAATGAAA+AGG + chr3_4:9761093-9761112 Msa0489420:intron 15.0%
!!! TTGTTTAATATTTCATTTTC+AGG + chr3_4:9761148-9761167 Msa0489420:intron 15.0%
!! ATAGCTCATAAAATTATAAA+TGG + chr3_4:9761710-9761729 Msa0489420:intron 15.0%
!! TAGCTCATAAAATTATAAAT+GGG + chr3_4:9761711-9761730 Msa0489420:intron 15.0%
!!! TATTTATGTGGATTTTAAAT+TGG + chr3_4:9762546-9762565 Msa0489420:CDS 15.0%
!! CTAAAAAAGTAAATAATTTC+TGG - chr3_4:9762631-9762650 None:intergenic 15.0%
!!! AGAAGATTAATTCATTTTTA+TGG + chr3_4:9763258-9763277 Msa0489420:intron 15.0%
!!! TTTCAAGTATTTTTTTTCAT+TGG + chr3_4:9763960-9763979 Msa0489420:intron 15.0%
!! TTTATATGTTAAAATCTTAC+CGG + chr3_4:9764373-9764392 Msa0489420:three_prime_UTR 15.0%
!!! ATAAATAAGTGTTTTGAATT+TGG - chr3_4:9764943-9764962 None:intergenic 15.0%
!! GGAAGAAAAAAACAATAAAA+AGG - chr3_4:9760405-9760424 None:intergenic 20.0%
!! GAAATAAAATTCACAAAGAA+AGG - chr3_4:9760595-9760614 None:intergenic 20.0%
!! TTTGGATAAACAACTTAATT+AGG + chr3_4:9760697-9760716 Msa0489420:intron 20.0%
!! CACAAAATAATGAGTAAATT+TGG - chr3_4:9761197-9761216 None:intergenic 20.0%
!! TAACATGTAAATTATGATCT+AGG - chr3_4:9761451-9761470 None:intergenic 20.0%
!! TCATAAAATTATAAATGGGA+CGG + chr3_4:9761715-9761734 Msa0489420:intron 20.0%
!! TCTGAAAATGAGTTTATAAA+TGG + chr3_4:9761934-9761953 Msa0489420:intron 20.0%
!! CTGAAAATGAGTTTATAAAT+GGG + chr3_4:9761935-9761954 Msa0489420:intron 20.0%
!! TAAATTCTAAGTAGACTATA+TGG + chr3_4:9762010-9762029 Msa0489420:intron 20.0%
!! TAAATATGATGTGTACATAA+CGG - chr3_4:9762144-9762163 None:intergenic 20.0%
!!! TTTTTTTCTCCTTATATCAA+AGG + chr3_4:9762862-9762881 Msa0489420:three_prime_UTR 20.0%
!! AAAGAAAGAAAACTTACATT+TGG - chr3_4:9762954-9762973 None:intergenic 20.0%
!!! TTAATGTCTCTTTTAAAGTT+TGG + chr3_4:9763089-9763108 Msa0489420:intron 20.0%
!!! ATGATTTTTCTTGAATTGTA+AGG + chr3_4:9763140-9763159 Msa0489420:intron 20.0%
!!! TGATTTTTCTTGAATTGTAA+GGG + chr3_4:9763141-9763160 Msa0489420:intron 20.0%
!!! GATAACTTGTTTAAAAACAT+TGG - chr3_4:9763347-9763366 None:intergenic 20.0%
!!! TGTTTTTAAACAAGTTATCA+AGG + chr3_4:9763348-9763367 Msa0489420:three_prime_UTR 20.0%
!! GAAATACTATAATTAGCAAA+CGG - chr3_4:9763420-9763439 None:intergenic 20.0%
!! AGAACATAAAAGATCATAAA+AGG - chr3_4:9763702-9763721 None:intergenic 20.0%
!!! TTTTATGTTCTGTTATCATT+AGG + chr3_4:9763711-9763730 Msa0489420:intron 20.0%
!!! ACAATGTTTTAGTGTATATT+CGG - chr3_4:9764546-9764565 None:intergenic 20.0%
!!! CTACAAAATGAATTTAACTT+TGG + chr3_4:9764754-9764773 Msa0489420:three_prime_UTR 20.0%
! TAACAGTTATGTTATCTTAC+AGG + chr3_4:9760182-9760201 Msa0489420:five_prime_UTR 25.0%
! AACAGTTATGTTATCTTACA+GGG + chr3_4:9760183-9760202 Msa0489420:five_prime_UTR 25.0%
! AAACAATAAAAAGGAGAAAG+AGG - chr3_4:9760396-9760415 None:intergenic 25.0%
! AGTTATAAAGAACAAGACAA+TGG - chr3_4:9760426-9760445 None:intergenic 25.0%
!!! CTTATTTCTATAAGTTCTGT+AGG - chr3_4:9760801-9760820 None:intergenic 25.0%
!!! GAGAGGTTTTAAATTAAAGT+AGG + chr3_4:9760964-9760983 Msa0489420:intron 25.0%
!! TATAAAGACCTTTTCATAAG+TGG - chr3_4:9761001-9761020 None:intergenic 25.0%
! CTTATGAAAAGGTCTTTATA+AGG + chr3_4:9761001-9761020 Msa0489420:intron 25.0%
! TATAAGGTTATAGATGTTCA+TGG + chr3_4:9761017-9761036 Msa0489420:intron 25.0%
! TCAAATAAAAATCACATGCA+TGG - chr3_4:9761289-9761308 None:intergenic 25.0%
! TGAAATCCGTTTCTATTTAT+AGG + chr3_4:9761337-9761356 Msa0489420:intron 25.0%
! GAAATCCGTTTCTATTTATA+GGG + chr3_4:9761338-9761357 Msa0489420:intron 25.0%
! TTGTTCCCTATAAATAGAAA+CGG - chr3_4:9761346-9761365 None:intergenic 25.0%
! CATAAAATTATAAATGGGAC+GGG + chr3_4:9761716-9761735 Msa0489420:intron 25.0%
! ATAAAATTATAAATGGGACG+GGG + chr3_4:9761717-9761736 Msa0489420:intron 25.0%
!!! TTACATATTCCATTTGGTTT+TGG - chr3_4:9762050-9762069 None:intergenic 25.0%
! TTCAACTTACATATTCCATT+TGG - chr3_4:9762056-9762075 None:intergenic 25.0%
! AATGTCAATCCTTTGATATA+AGG - chr3_4:9762874-9762893 None:intergenic 25.0%
! ATATTGCTGCAAAAAAATTG+AGG + chr3_4:9762912-9762931 Msa0489420:three_prime_UTR 25.0%
! AATGAAGACATGCATAAAAA+AGG + chr3_4:9763062-9763081 Msa0489420:intron 25.0%
! AGAAAAATCATGTCTTAACA+TGG - chr3_4:9763131-9763150 None:intergenic 25.0%
!! GATTTTTCTTGAATTGTAAG+GGG + chr3_4:9763142-9763161 Msa0489420:intron 25.0%
!! AATACACAGTTTAGTTTAAC+AGG + chr3_4:9763204-9763223 Msa0489420:intron 25.0%
!! ACCAAAAGACATTTTATTAG+AGG + chr3_4:9763309-9763328 Msa0489420:three_prime_UTR 25.0%
!! ACCTCTAATAAAATGTCTTT+TGG - chr3_4:9763313-9763332 None:intergenic 25.0%
! ACTTGTTTAAAAACATTGGA+AGG - chr3_4:9763343-9763362 None:intergenic 25.0%
! AACATAGCTATGTAATTTAC+TGG - chr3_4:9763458-9763477 None:intergenic 25.0%
! TGACAAATTTGAATGTGTAT+TGG - chr3_4:9763801-9763820 None:intergenic 25.0%
! AGAAGGATATAAACCAATAA+TGG - chr3_4:9763829-9763848 None:intergenic 25.0%
!!! ATTATTGGTTTATATCCTTC+TGG + chr3_4:9763828-9763847 Msa0489420:intron 25.0%
! ACAGTGAAGATTTAATTGAA+TGG - chr3_4:9763891-9763910 None:intergenic 25.0%
!! TTTTGCTTGTTCTGTTAAAA+CGG + chr3_4:9764196-9764215 Msa0489420:intron 25.0%
!!! AATCATTTTAAGAGATTCCT+TGG - chr3_4:9764428-9764447 None:intergenic 25.0%
! TCTCTTAAAATGATTCAAAG+TGG + chr3_4:9764433-9764452 Msa0489420:three_prime_UTR 25.0%
!! AATGTAGTGGTATTTTCTTT+TGG + chr3_4:9764798-9764817 Msa0489420:three_prime_UTR 25.0%
TTGCTTAAATCAAGTGTTTG+AGG + chr3_4:9760247-9760266 Msa0489420:five_prime_UTR 30.0%
ACAACACAGAATTTCAAATG+AGG - chr3_4:9760299-9760318 None:intergenic 30.0%
! TGGAGTTAGATTTAAGATGA+AGG - chr3_4:9760359-9760378 None:intergenic 30.0%
TAAAAAGGAGAAAGAGGAAA+AGG - chr3_4:9760390-9760409 None:intergenic 30.0%
!!! GTAGAAGATTTTGTTTTGTG+TGG + chr3_4:9760484-9760503 Msa0489420:CDS 30.0%
! TTCTTGACTTAGGACATTTT+TGG + chr3_4:9760679-9760698 Msa0489420:intron 30.0%
AATAAGCAGAAAACAACTCA+TGG + chr3_4:9760815-9760834 Msa0489420:intron 30.0%
GCTTAAAGCTAATCCAAAAA+GGG - chr3_4:9760940-9760959 None:intergenic 30.0%
AGCTTAAAGCTAATCCAAAA+AGG - chr3_4:9760941-9760960 None:intergenic 30.0%
!! ATTAGCTTTAAGCTTATGAG+AGG + chr3_4:9760947-9760966 Msa0489420:intron 30.0%
! GAAGTTTTCCACTTATGAAA+AGG + chr3_4:9760990-9761009 Msa0489420:intron 30.0%
ATGTTCATGGAGAAGTTAAA+CGG + chr3_4:9761030-9761049 Msa0489420:intron 30.0%
GTTCAAAAACTTCATAGTTG+TGG + chr3_4:9761120-9761139 Msa0489420:intron 30.0%
! TGATGATGTTAGAGAACTAA+AGG + chr3_4:9761562-9761581 Msa0489420:CDS 30.0%
ACGTTTAAATAGTAAGAGAG+AGG + chr3_4:9761607-9761626 Msa0489420:intron 30.0%
CGTTTAAATAGTAAGAGAGA+GGG + chr3_4:9761608-9761627 Msa0489420:intron 30.0%
GGAGAAAAATCATAGAAGAT+AGG + chr3_4:9761645-9761664 Msa0489420:intron 30.0%
AATCATAGAAGATAGGCAAA+AGG + chr3_4:9761652-9761671 Msa0489420:intron 30.0%
ATCATAGAAGATAGGCAAAA+GGG + chr3_4:9761653-9761672 Msa0489420:intron 30.0%
AAAACCAACTTCTATAGTGA+GGG - chr3_4:9761754-9761773 None:intergenic 30.0%
!!! ACTATAGAAGTTGGTTTTGT+TGG + chr3_4:9761756-9761775 Msa0489420:intron 30.0%
TTCTAGCCATCTTATAACTT+CGG - chr3_4:9761798-9761817 None:intergenic 30.0%
! ATGTGGATTTTAAATTGGCA+TGG + chr3_4:9762551-9762570 Msa0489420:CDS 30.0%
ACGTTAGTAGATATCATGAA+AGG + chr3_4:9762666-9762685 Msa0489420:three_prime_UTR 30.0%
!! TAGATATCATGAAAGGTACT+TGG + chr3_4:9762673-9762692 Msa0489420:three_prime_UTR 30.0%
!! AGATATCATGAAAGGTACTT+GGG + chr3_4:9762674-9762693 Msa0489420:three_prime_UTR 30.0%
! AATATCTGGTTAGTCAAACT+TGG - chr3_4:9762706-9762725 None:intergenic 30.0%
ATGATTATTATCAGACTTGC+TGG - chr3_4:9762787-9762806 None:intergenic 30.0%
! ATGTAAAGTACAAAGTACTC+TGG - chr3_4:9763556-9763575 None:intergenic 30.0%
! AAGGAAAAACTGACAGATTT+TGG - chr3_4:9763683-9763702 None:intergenic 30.0%
ATTTGTCACATCACCATTAT+TGG + chr3_4:9763813-9763832 Msa0489420:intron 30.0%
ACTGTACTAAAAAAACCAGA+AGG - chr3_4:9763846-9763865 None:intergenic 30.0%
!!! TCTGGTTTTTTTAGTACAGT+TGG + chr3_4:9763846-9763865 Msa0489420:intron 30.0%
!! TTTTTAGTACAGTTGGTGTT+TGG + chr3_4:9763853-9763872 Msa0489420:intron 30.0%
! AAAATACTTGAAAGAGCACA+TGG - chr3_4:9763953-9763972 None:intergenic 30.0%
! TGCTGATTGTAGAAAATAGT+GGG - chr3_4:9764137-9764156 None:intergenic 30.0%
! TTGCTGATTGTAGAAAATAG+TGG - chr3_4:9764138-9764157 None:intergenic 30.0%
AGATCTTGAAACTACATTTG+AGG + chr3_4:9764291-9764310 Msa0489420:three_prime_UTR 30.0%
TATGTTAAAATCTTACCGGA+TGG + chr3_4:9764377-9764396 Msa0489420:three_prime_UTR 30.0%
TGTCATCTAAGTGAAAATCA+CGG + chr3_4:9764485-9764504 Msa0489420:three_prime_UTR 30.0%
! CTTTTCATAAACTGAAACTG+TGG - chr3_4:9764591-9764610 None:intergenic 30.0%
! AAGCTATGACTTCTGATTTT+CGG + chr3_4:9764629-9764648 Msa0489420:three_prime_UTR 30.0%
! TTTTCGGCATGCATATTTTA+AGG + chr3_4:9764645-9764664 Msa0489420:three_prime_UTR 30.0%
TGAGAAACACAAATGTTCTT+TGG - chr3_4:9764862-9764881 None:intergenic 30.0%
AGAACATTTGTGTTTCTCAA+AGG + chr3_4:9764863-9764882 Msa0489420:three_prime_UTR 30.0%
AGTTATGTTATCTTACAGGG+TGG + chr3_4:9760186-9760205 Msa0489420:five_prime_UTR 35.0%
! TAAATCAAGTGTTTGAGGTG+CGG + chr3_4:9760252-9760271 Msa0489420:five_prime_UTR 35.0%
GTGCGGTTATTATCATATAG+AGG + chr3_4:9760269-9760288 Msa0489420:five_prime_UTR 35.0%
CATCTTAAATCTAACTCCAC+AGG + chr3_4:9760360-9760379 Msa0489420:CDS 35.0%
!! TTCTGTCTTTCTTTGAAGCA+TGG + chr3_4:9760523-9760542 Msa0489420:CDS 35.0%
!! TCTGTCTTTCTTTGAAGCAT+GGG + chr3_4:9760524-9760543 Msa0489420:CDS 35.0%
ATACTATGTAACATGACCTG+AGG + chr3_4:9760637-9760656 Msa0489420:intron 35.0%
TACTATGTAACATGACCTGA+GGG + chr3_4:9760638-9760657 Msa0489420:intron 35.0%
GAACGTGATATTCTTGACTT+AGG + chr3_4:9760669-9760688 Msa0489420:intron 35.0%
AGACTATTTGAGAGAGCTTA+AGG - chr3_4:9760859-9760878 None:intergenic 35.0%
TATAATCCAAACAGTTCCGT+AGG + chr3_4:9760914-9760933 Msa0489420:intron 35.0%
TGTTCATGGAGAAGTTAAAC+GGG + chr3_4:9761031-9761050 Msa0489420:intron 35.0%
TCACATTCTTTGATTGCTCA+GGG + chr3_4:9761243-9761262 Msa0489420:intron 35.0%
! AATTTCTTCTGCTCGTTTTG+AGG + chr3_4:9761514-9761533 Msa0489420:CDS 35.0%
TTAAATAGTAAGAGAGAGGG+AGG + chr3_4:9761611-9761630 Msa0489420:intron 35.0%
TAAATAGTAAGAGAGAGGGA+GGG + chr3_4:9761612-9761631 Msa0489420:intron 35.0%
! AGGGAATCAAAGGCATAATA+CGG + chr3_4:9761672-9761691 Msa0489420:intron 35.0%
CAAAACCAACTTCTATAGTG+AGG - chr3_4:9761755-9761774 None:intergenic 35.0%
AAAGTCCATCAAAGATCCAT+TGG + chr3_4:9761816-9761835 Msa0489420:intron 35.0%
AAGTCCATCAAAGATCCATT+GGG + chr3_4:9761817-9761836 Msa0489420:intron 35.0%
! GTGTTTTACGAGTTCTACAT+TGG + chr3_4:9761900-9761919 Msa0489420:intron 35.0%
AATCCTACCAAATAGACTTG+TGG - chr3_4:9761975-9761994 None:intergenic 35.0%
TGAGTTAGACCAAAACCAAA+TGG + chr3_4:9762038-9762057 Msa0489420:intron 35.0%
! TAAGTTGAAGTACAGCTGAT+TGG + chr3_4:9762067-9762086 Msa0489420:intron 35.0%
GTCATCACATCTAACTTCTA+AGG + chr3_4:9762344-9762363 Msa0489420:intron 35.0%
CGATGATGTGTTTCAGAAAA+TGG + chr3_4:9762472-9762491 Msa0489420:CDS 35.0%
! CTTTTACTCTACGAAATCCT+GGG - chr3_4:9762517-9762536 None:intergenic 35.0%
! AAGGAGGTGAAGTATTTATG+TGG + chr3_4:9762534-9762553 Msa0489420:CDS 35.0%
! TGTTCTCTTTTCCTTTGCTA+AGG - chr3_4:9762597-9762616 None:intergenic 35.0%
!! GATATCATGAAAGGTACTTG+GGG + chr3_4:9762675-9762694 Msa0489420:three_prime_UTR 35.0%
! TTCGAAAGAAAACCGTTTTG+AGG - chr3_4:9762837-9762856 None:intergenic 35.0%
CAAAAAAATTGAGGACAACC+AGG + chr3_4:9762921-9762940 Msa0489420:three_prime_UTR 35.0%
!!! GACATTTTATTAGAGGTGCT+TGG + chr3_4:9763316-9763335 Msa0489420:three_prime_UTR 35.0%
GGAAATAATCAACTCAACTG+TGG + chr3_4:9763369-9763388 Msa0489420:three_prime_UTR 35.0%
AGAACATCTTCTGTTGTCTA+AGG + chr3_4:9763652-9763671 Msa0489420:intron 35.0%
TGTGTATTGGAAACCCTATT+CGG - chr3_4:9763788-9763807 None:intergenic 35.0%
!! CACAAAGTGTTGTCAAATAG+TGG + chr3_4:9764067-9764086 Msa0489420:intron 35.0%
TTGTCAAATAGTGGCTATAG+CGG + chr3_4:9764076-9764095 Msa0489420:intron 35.0%
CTGTTAAAACGGATATTTGC+TGG + chr3_4:9764207-9764226 Msa0489420:intron 35.0%
TTTGCTGGAAAATTCTTTGC+AGG + chr3_4:9764222-9764241 Msa0489420:intron 35.0%
CTTGAAACTACATTTGAGGA+AGG + chr3_4:9764295-9764314 Msa0489420:three_prime_UTR 35.0%
GCTTCAAAAGTAGAATTGAC+AGG + chr3_4:9764348-9764367 Msa0489420:three_prime_UTR 35.0%
ACATTGTTTCCTCCTAATGA+TGG + chr3_4:9764559-9764578 Msa0489420:three_prime_UTR 35.0%
! TATGTTTTCTCACGCATTGA+TGG - chr3_4:9764687-9764706 None:intergenic 35.0%
ATCAATGCGTGAGAAAACAT+AGG + chr3_4:9764686-9764705 Msa0489420:three_prime_UTR 35.0%
ATCTCTTGAAAGCAATGTAG+TGG + chr3_4:9764785-9764804 Msa0489420:three_prime_UTR 35.0%
TTTGGAGAAGAGAAATGCTA+GGG - chr3_4:9764925-9764944 None:intergenic 35.0%
ATTTGGAGAAGAGAAATGCT+AGG - chr3_4:9764926-9764945 None:intergenic 35.0%
AAGAGGAAAAGGTTAACCTG+TGG - chr3_4:9760379-9760398 None:intergenic 40.0%
GCTTGCAGATGATAAAACAC+AGG + chr3_4:9760455-9760474 Msa0489420:intron 40.0%
CTCACATTCTTTGATTGCTC+AGG + chr3_4:9761242-9761261 Msa0489420:intron 40.0%
GCAGACAGAGAAAGAAAAAC+TGG - chr3_4:9761406-9761425 None:intergenic 40.0%
GATAGGCAAAAGGGAATCAA+AGG + chr3_4:9761662-9761681 Msa0489420:intron 40.0%
! GAATCAAAGGCATAATACGG+TGG + chr3_4:9761675-9761694 Msa0489420:intron 40.0%
TTATAAATGGGACGGGGTTA+TGG + chr3_4:9761723-9761742 Msa0489420:intron 40.0%
TCAACCCTCACTATAGAAGT+TGG + chr3_4:9761747-9761766 Msa0489420:intron 40.0%
GCCATCTTATAACTTCGGTT+GGG - chr3_4:9761793-9761812 None:intergenic 40.0%
AGCCATCTTATAACTTCGGT+TGG - chr3_4:9761794-9761813 None:intergenic 40.0%
CAAATAGACTTGTGGAGAGA+GGG - chr3_4:9761967-9761986 None:intergenic 40.0%
TCTCCACAAGTCTATTTGGT+AGG + chr3_4:9761969-9761988 Msa0489420:intron 40.0%
TTGAAGTACAGCTGATTGGT+AGG + chr3_4:9762071-9762090 Msa0489420:intron 40.0%
CGACAAAAGATCGAATTGCA+AGG + chr3_4:9762245-9762264 Msa0489420:intron 40.0%
TATCTGGAAACAGAACACAG+AGG + chr3_4:9762444-9762463 Msa0489420:CDS 40.0%
! CCTTTTACTCTACGAAATCC+TGG - chr3_4:9762518-9762537 None:intergenic 40.0%
CCAGGATTTCGTAGAGTAAA+AGG + chr3_4:9762515-9762534 Msa0489420:CDS 40.0%
GGATTTCGTAGAGTAAAAGG+AGG + chr3_4:9762518-9762537 Msa0489420:CDS 40.0%
! CAGATTGATAGCCTTAGCAA+AGG + chr3_4:9762583-9762602 Msa0489420:CDS 40.0%
GACTAACCAGATATTACGTG+CGG + chr3_4:9762711-9762730 Msa0489420:three_prime_UTR 40.0%
AAACTTCCGCACGTAATATC+TGG - chr3_4:9762720-9762739 None:intergenic 40.0%
!!! ATCAGACTTGCTGGTGATTT+TGG - chr3_4:9762778-9762797 None:intergenic 40.0%
TGTCATCTGACTCCTCAAAA+CGG + chr3_4:9762822-9762841 Msa0489420:three_prime_UTR 40.0%
! GACATTTTTCACGTGCTTCT+TGG + chr3_4:9762888-9762907 Msa0489420:three_prime_UTR 40.0%
! CTTACATTTGGCGTTTTTCC+TGG - chr3_4:9762942-9762961 None:intergenic 40.0%
! TTCATTGATACACACCGACT+TGG - chr3_4:9763048-9763067 None:intergenic 40.0%
!! ACCATGCTTGCTATTACACA+AGG + chr3_4:9763523-9763542 Msa0489420:intron 40.0%
TCCTTGTGTAATAGCAAGCA+TGG - chr3_4:9763527-9763546 None:intergenic 40.0%
ATGCTACCTAAAAGGCTTCA+AGG - chr3_4:9763596-9763615 None:intergenic 40.0%
!! GAAGCCTTTTAGGTAGCATT+TGG + chr3_4:9763597-9763616 Msa0489420:intron 40.0%
ACATCTTCTGTTGTCTAAGG+TGG + chr3_4:9763655-9763674 Msa0489420:intron 40.0%
AAATATCCTGCCTACCGAAT+AGG + chr3_4:9763771-9763790 Msa0489420:intron 40.0%
AATATCCTGCCTACCGAATA+GGG + chr3_4:9763772-9763791 Msa0489420:intron 40.0%
TATTGGAAACCCTATTCGGT+AGG - chr3_4:9763784-9763803 None:intergenic 40.0%
TGGTCAGAGTTGTCAAATAG+TGG + chr3_4:9763980-9763999 Msa0489420:intron 40.0%
GCTATAGCACTATAGTACAG+TGG + chr3_4:9764004-9764023 Msa0489420:intron 40.0%
! TTTGTGCTGATGGCATATAG+CGG - chr3_4:9764053-9764072 None:intergenic 40.0%
TTGACAACACTTTGTGCTGA+TGG - chr3_4:9764063-9764082 None:intergenic 40.0%
ATGGAGTGACATTGCTTCTT+CGG + chr3_4:9764396-9764415 Msa0489420:three_prime_UTR 40.0%
CATCTAAGTGAAAATCACGG+TGG + chr3_4:9764488-9764507 Msa0489420:three_prime_UTR 40.0%
GTAGTTCTCAAAATCAACCG+CGG - chr3_4:9764738-9764757 None:intergenic 40.0%
!! CTTTCTTTGAAGCATGGGCA+CGG + chr3_4:9760529-9760548 Msa0489420:CDS 45.0%
!! GTGCAGCGAGGATTTTGAAA+AGG + chr3_4:9760551-9760570 Msa0489420:intron 45.0%
GCTAATCCAAAAAGGGCCTA+CGG - chr3_4:9760933-9760952 None:intergenic 45.0%
GGAGAAGTTAAACGGGACTA+CGG + chr3_4:9761038-9761057 Msa0489420:intron 45.0%
GAGAAGTTAAACGGGACTAC+GGG + chr3_4:9761039-9761058 Msa0489420:intron 45.0%
CCTGATAACACAGAAGCAGT+AGG - chr3_4:9761381-9761400 None:intergenic 45.0%
CCTACTGCTTCTGTGTTATC+AGG + chr3_4:9761378-9761397 Msa0489420:intron 45.0%
GCCCAACCGAAGTTATAAGA+TGG + chr3_4:9761789-9761808 Msa0489420:intron 45.0%
GTGTCCCAATGGATCTTTGA+TGG - chr3_4:9761824-9761843 None:intergenic 45.0%
CCAAATAGACTTGTGGAGAG+AGG - chr3_4:9761968-9761987 None:intergenic 45.0%
CCTCTCTCCACAAGTCTATT+TGG + chr3_4:9761965-9761984 Msa0489420:intron 45.0%
! TTCTGTCCAGATAAGCATCG+AGG + chr3_4:9762421-9762440 Msa0489420:intron 45.0%
CAGATACCTCGATGCTTATC+TGG - chr3_4:9762430-9762449 None:intergenic 45.0%
!! CAGATAAGCATCGAGGTATC+TGG + chr3_4:9762428-9762447 Msa0489420:intron 45.0%
ACTCTACGAAATCCTGGGAT+CGG - chr3_4:9762512-9762531 None:intergenic 45.0%
! ACGTGCGGAAGTTTTCTGTT+TGG + chr3_4:9762726-9762745 Msa0489420:three_prime_UTR 45.0%
AACAACCTTGACCACCAAGT+CGG + chr3_4:9763031-9763050 Msa0489420:intron 45.0%
!! ATTGATACACACCGACTTGG+TGG - chr3_4:9763045-9763064 None:intergenic 45.0%
AAAAGGCTTCAAGGTGGCTA+TGG - chr3_4:9763587-9763606 None:intergenic 45.0%
! TAGCCACCTTGAAGCCTTTT+AGG + chr3_4:9763587-9763606 Msa0489420:intron 45.0%
CTACCTAAAAGGCTTCAAGG+TGG - chr3_4:9763593-9763612 None:intergenic 45.0%
CACGCCAAATGCTACCTAAA+AGG - chr3_4:9763604-9763623 None:intergenic 45.0%
AGAAGCAATGTCACTCCATC+CGG - chr3_4:9764395-9764414 None:intergenic 45.0%
AAAATCACGGTGGCATTTCG+CGG + chr3_4:9764498-9764517 Msa0489420:three_prime_UTR 45.0%
TGGTGTTCACCATCATTAGG+AGG - chr3_4:9764571-9764590 None:intergenic 45.0%
CTGTGGTGTTCACCATCATT+AGG - chr3_4:9764574-9764593 None:intergenic 45.0%
GGACATCACTCTACAAACTC+TGG + chr3_4:9764707-9764726 Msa0489420:three_prime_UTR 45.0%
AAAGGGCCTACGGAACTGTT+TGG - chr3_4:9760923-9760942 None:intergenic 50.0%
! ACAGTTCCGTAGGCCCTTTT+TGG + chr3_4:9760924-9760943 Msa0489420:intron 50.0%
CACATGCATGGTGACATGTG+AGG - chr3_4:9761277-9761296 None:intergenic 50.0%
ATAGTAAGAGAGAGGGAGGG+AGG + chr3_4:9761615-9761634 Msa0489420:intron 50.0%
TAGTAAGAGAGAGGGAGGGA+GGG + chr3_4:9761616-9761635 Msa0489420:intron 50.0%
TCCATTGGGACACCCACTAT+CGG + chr3_4:9761831-9761850 Msa0489420:intron 50.0%
TGCTCCAGATGTCCAATCCT+AGG + chr3_4:9761867-9761886 Msa0489420:intron 50.0%
ATGTCCAATCCTAGGCGTGA+GGG + chr3_4:9761875-9761894 Msa0489420:intron 50.0%
CTCAACTGTGGCTGAGTATG+TGG + chr3_4:9763381-9763400 Msa0489420:three_prime_UTR 50.0%
TGTGGCTGAGTATGTGGAGA+AGG + chr3_4:9763387-9763406 Msa0489420:three_prime_UTR 50.0%
TGCTTCTTCGGTCTGAACCA+AGG + chr3_4:9764408-9764427 Msa0489420:three_prime_UTR 50.0%
GTAACATGACCTGAGGGCTG+AGG + chr3_4:9760644-9760663 Msa0489420:intron 55.0%
CCATTGGGACACCCACTATC+GGG + chr3_4:9761832-9761851 Msa0489420:intron 55.0%
CACGCCTAGGATTGGACATC+TGG - chr3_4:9761874-9761893 None:intergenic 55.0%
GATGTCCAATCCTAGGCGTG+AGG + chr3_4:9761874-9761893 Msa0489420:intron 55.0%
TGTCCAATCCTAGGCGTGAG+GGG + chr3_4:9761876-9761895 Msa0489420:intron 55.0%
AAAACACACCCCTCACGCCT+AGG - chr3_4:9761887-9761906 None:intergenic 55.0%
AAAGCTGCACAACCGATCCC+AGG + chr3_4:9762497-9762516 Msa0489420:CDS 55.0%
!! ACACACCGACTTGGTGGTCA+AGG - chr3_4:9763039-9763058 None:intergenic 55.0%
GGAAACCCTATTCGGTAGGC+AGG - chr3_4:9763780-9763799 None:intergenic 55.0%
!! TACAAACTCTGGAGCAGCCG+CGG + chr3_4:9764718-9764737 Msa0489420:three_prime_UTR 55.0%
TAAGAGAGAGGGAGGGAGGG+AGG + chr3_4:9761619-9761638 Msa0489420:intron 60.0%
AAGAGAGAGGGAGGGAGGGA+GGG + chr3_4:9761620-9761639 Msa0489420:intron 60.0%
!! CCCGATAGTGGGTGTCCCAA+TGG - chr3_4:9761835-9761854 None:intergenic 60.0%
GACCTGAGCAGCCCGATAGT+GGG - chr3_4:9761846-9761865 None:intergenic 60.0%
TGACCTGAGCAGCCCGATAG+TGG - chr3_4:9761847-9761866 None:intergenic 60.0%
ACACCCCTCACGCCTAGGAT+TGG - chr3_4:9761882-9761901 None:intergenic 60.0%
CACGTTCAGCCTCAGCCCTC+AGG - chr3_4:9760656-9760675 None:intergenic 65.0%
CACCCACTATCGGGCTGCTC+AGG + chr3_4:9761841-9761860 Msa0489420:intron 65.0%
AGCATGGGCACGGTGCAGCG+AGG + chr3_4:9760539-9760558 Msa0489420:intron 70.0%
AGAGAGGGAGGGAGGGAGGG+AGG + chr3_4:9761623-9761642 Msa0489420:intron 70.0%
GAGAGGGAGGGAGGGAGGGA+GGG + chr3_4:9761624-9761643 Msa0489420:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 9760175 9764962 9760175 ID=Msa0489420;Name=Msa0489420
chr3_4 mRNA 9760175 9764962 9760175 ID=Msa0489420-mRNA-1;Parent=Msa0489420;Name=Msa0489420-mRNA-1;_AED=0.07;_eAED=0.07;_QI=162|0.8|0.83|1|0.8|0.83|6|1122|144
chr3_4 exon 9760175 9760381 9760175 ID=Msa0489420-mRNA-1:exon:3147;Parent=Msa0489420-mRNA-1
chr3_4 exon 9760463 9760550 9760463 ID=Msa0489420-mRNA-1:exon:3148;Parent=Msa0489420-mRNA-1
chr3_4 exon 9761507 9761583 9761507 ID=Msa0489420-mRNA-1:exon:3149;Parent=Msa0489420-mRNA-1
chr3_4 exon 9762431 9762956 9762431 ID=Msa0489420-mRNA-1:exon:3150;Parent=Msa0489420-mRNA-1
chr3_4 exon 9763307 9763408 9763307 ID=Msa0489420-mRNA-1:exon:3151;Parent=Msa0489420-mRNA-1
chr3_4 exon 9764244 9764962 9764244 ID=Msa0489420-mRNA-1:exon:3152;Parent=Msa0489420-mRNA-1
chr3_4 five_prime_UTR 9760175 9760336 9760175 ID=Msa0489420-mRNA-1:five_prime_utr;Parent=Msa0489420-mRNA-1
chr3_4 CDS 9760337 9760381 9760337 ID=Msa0489420-mRNA-1:cds;Parent=Msa0489420-mRNA-1
chr3_4 CDS 9760463 9760550 9760463 ID=Msa0489420-mRNA-1:cds;Parent=Msa0489420-mRNA-1
chr3_4 CDS 9761507 9761583 9761507 ID=Msa0489420-mRNA-1:cds;Parent=Msa0489420-mRNA-1
chr3_4 CDS 9762431 9762655 9762431 ID=Msa0489420-mRNA-1:cds;Parent=Msa0489420-mRNA-1
chr3_4 three_prime_UTR 9762656 9762956 9762656 ID=Msa0489420-mRNA-1:three_prime_utr;Parent=Msa0489420-mRNA-1
chr3_4 three_prime_UTR 9763307 9763408 9763307 ID=Msa0489420-mRNA-1:three_prime_utr;Parent=Msa0489420-mRNA-1
chr3_4 three_prime_UTR 9764244 9764962 9764244 ID=Msa0489420-mRNA-1:three_prime_utr;Parent=Msa0489420-mRNA-1
Gene Sequence

>Msa0489420

ATGCATACATCATCTCAAACCTTCATCTTAAATCTAACTCCACAGATGATAAAACACAGGCAGAGAGTAGAAGATTTTGTTTTGTGTGGCTCGAATTCTAGACATTTCTGTCTTTCTTTGAAGCATGGGCACGATACTGAAATTTCTTCTGCTCGTTTTGAGGAGTTCTCTGTCTCTACTGTTGACACTGATGATGTTAGAGAACTAAAGATAAGCATCGAGGTATCTGGAAACAGAACACAGAGGACTTTCGATGATGTGTTTCAGAAAATGGTAAAAGCTGCACAACCGATCCCAGGATTTCGTAGAGTAAAAGGAGGTGAAGTATTTATGTGGATTTTAAATTGGCATGGTTTATTCATCAGATTGATAGCCTTAGCAAAGGAAAAGAGAACAGAACAAGAAGTCCAGAAATTATTTACTTTTTTAGCATAA
Protein sequence

>Msa0489420

MHTSSQTFILNLTPQMIKHRQRVEDFVLCGSNSRHFCLSLKHGHDTEISSARFEEFSVSTVDTDDVRELKISIEVSGNRTQRTFDDVFQKMVKAAQPIPGFRRVKGGEVFMWILNWHGLFIRLIALAKEKRTEQEVQKLFTFLA*