AlfalfaGEDB | Msa0495830

M. sativa cultivar zhongmu-4 / Msa0495830

KEGG: K03086
GO: GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141
PFAM: PF04539.19

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0495830	XP_039688195.1	90.909	110	8	2	1	108	1	110	9.84e-53	173

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0495830	sp\|Q9ZSL6\|SIGD_ARATH	51.351	111	52	2	1	109	263	373	1.11e-27	108

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0495830	G7J0W0	90.909	110	8	2	1	108	1	110	4.70e-53	173

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0436460	Msa0495830	0.825838	3.768585e-54	-8.615850e-47
Msa0280490	Msa0495830	0.813021	3.159995e-51	-8.615850e-47
Msa0354370	Msa0495830	0.847338	1.232119e-59	-8.615850e-47
Msa0495830	Msa0496000	0.897554	1.256407e-76	-8.615850e-47
Msa0495830	Msa0706370	0.812600	3.906581e-51	-8.615850e-47
Msa0495830	Msa0898370	0.801535	8.600398e-49	-8.615850e-47
Msa0495830	Msa1020720	0.809442	1.888003e-50	-8.615850e-47
Msa0495830	Msa1139410	0.801579	8.425087e-49	-8.615850e-47
Msa0495830	Msa1379990	0.831781	1.374293e-55	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0495830	MtrunA17_Chr3g0090001	90.909	110	8	2	1	108	250	359	7.34e-54	173
Msa0495830	MtrunA17_Chr3g0089951	90.816	98	8	1	1	97	1	98	1.26e-49	153

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0495830	AT5G13730.1	51.351	111	52	2	1	109	263	373	1.13e-28	108

Find 19 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATAGCTCTGTCCAATGAAAT+TGG	0.360695	3_4:-25484169	None:intergenic
TCCGGAATCGTTTCAGATCC+TGG	0.382220	3_4:-25484345	None:intergenic
ACCAGGATCTGAAACGATTC+CGG	0.390207	3_4:+25484344	Msa0495830:CDS
AGATTATACTTTGGACTCAA+TGG	0.428294	3_4:+25484447	Msa0495830:CDS
AAGTAGACAACCAATTTCAT+TGG	0.441740	3_4:+25484159	Msa0495830:CDS
GAGAGAATCTGACAGATTCA+TGG	0.482979	3_4:+25484469	Msa0495830:CDS
GAGATAGTAAGATTATACTT+TGG	0.511798	3_4:+25484438	Msa0495830:CDS
TGCCTCTCAACCATCTTCTC+CGG	0.517628	3_4:-25484363	None:intergenic
TGAAACGATTCCGGAGAAGA+TGG	0.534729	3_4:+25484353	Msa0495830:CDS
TCAGCAATTTCATTATATGT+CGG	0.554776	3_4:-25484091	None:intergenic
GAGAGGCAGTTAGTGACGCA+AGG	0.574565	3_4:+25484378	Msa0495830:CDS
TCAAAGCAACTTAATACTGA+AGG	0.589123	3_4:+25484198	Msa0495830:CDS
ATAATGCAGGAGATTATACC+AGG	0.599984	3_4:+25484327	Msa0495830:intron
AGAGGCAGTTAGTGACGCAA+GGG	0.600916	3_4:+25484379	Msa0495830:CDS
TTCCGGAGAAGATGGTTGAG+AGG	0.609116	3_4:+25484361	Msa0495830:CDS
GACATATAATGAAATTGCTG+AGG	0.644481	3_4:+25484093	Msa0495830:CDS
TCTTAAGACGAAGACTGAGG+CGG	0.681542	3_4:+25484065	Msa0495830:CDS
GAGGCAGTTAGTGACGCAAG+GGG	0.690313	3_4:+25484380	Msa0495830:CDS
ATGTCTTAAGACGAAGACTG+AGG	0.725315	3_4:+25484062	Msa0495830:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAGCAATTTCATTATATGT+CGG	-	chr3_4:25484094-25484113	None:intergenic	25.0%
!	TCTTTACTATGATATAATGC+AGG	+	chr3_4:25484314-25484333	Msa0495830:intron	25.0%
!	GAGATAGTAAGATTATACTT+TGG	+	chr3_4:25484438-25484457	Msa0495830:CDS	25.0%
	GACATATAATGAAATTGCTG+AGG	+	chr3_4:25484093-25484112	Msa0495830:CDS	30.0%
	AAGTAGACAACCAATTTCAT+TGG	+	chr3_4:25484159-25484178	Msa0495830:CDS	30.0%
	TCAAAGCAACTTAATACTGA+AGG	+	chr3_4:25484198-25484217	Msa0495830:CDS	30.0%
	AGATTATACTTTGGACTCAA+TGG	+	chr3_4:25484447-25484466	Msa0495830:CDS	30.0%
!	ATAGCTCTGTCCAATGAAAT+TGG	-	chr3_4:25484172-25484191	None:intergenic	35.0%
	ATAATGCAGGAGATTATACC+AGG	+	chr3_4:25484327-25484346	Msa0495830:intron	35.0%
	ATGTCTTAAGACGAAGACTG+AGG	+	chr3_4:25484062-25484081	Msa0495830:CDS	40.0%
	GAGAGAATCTGACAGATTCA+TGG	+	chr3_4:25484469-25484488	Msa0495830:CDS	40.0%
	TCTTAAGACGAAGACTGAGG+CGG	+	chr3_4:25484065-25484084	Msa0495830:CDS	45.0%
!	ACCAGGATCTGAAACGATTC+CGG	+	chr3_4:25484344-25484363	Msa0495830:CDS	45.0%
	TGAAACGATTCCGGAGAAGA+TGG	+	chr3_4:25484353-25484372	Msa0495830:CDS	45.0%
	TCCGGAATCGTTTCAGATCC+TGG	-	chr3_4:25484348-25484367	None:intergenic	50.0%
	TTCCGGAGAAGATGGTTGAG+AGG	+	chr3_4:25484361-25484380	Msa0495830:CDS	50.0%
	TGCCTCTCAACCATCTTCTC+CGG	-	chr3_4:25484366-25484385	None:intergenic	50.0%
	AGAGGCAGTTAGTGACGCAA+GGG	+	chr3_4:25484379-25484398	Msa0495830:CDS	50.0%
	GAGAGGCAGTTAGTGACGCA+AGG	+	chr3_4:25484378-25484397	Msa0495830:CDS	55.0%
	GAGGCAGTTAGTGACGCAAG+GGG	+	chr3_4:25484380-25484399	Msa0495830:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	25484034	25484533	25484034	ID=Msa0495830;Name=Msa0495830
chr3_4	mRNA	25484034	25484533	25484034	ID=Msa0495830-mRNA-1;Parent=Msa0495830;Name=Msa0495830-mRNA-1;_AED=0.03;_eAED=0.18;_QI=0\|0\|0\|1\|1\|1\|2\|0\|127
chr3_4	exon	25484034	25484219	25484034	ID=Msa0495830-mRNA-1:exon:6134;Parent=Msa0495830-mRNA-1
chr3_4	exon	25484336	25484533	25484336	ID=Msa0495830-mRNA-1:exon:6135;Parent=Msa0495830-mRNA-1
chr3_4	CDS	25484034	25484219	25484034	ID=Msa0495830-mRNA-1:cds;Parent=Msa0495830-mRNA-1
chr3_4	CDS	25484336	25484533	25484336	ID=Msa0495830-mRNA-1:cds;Parent=Msa0495830-mRNA-1

Gene Sequence

>Msa0495830

ATGGTTGCAAAAATCGCAGAGGCTAACAATGTCTTAAGACGAAGACTGAGGCGGAAGCCGACATATAATGAAATTGCTGAGGTTCTCAATGTGAATGTTTCAACTGTAAAACTTGTTTCTGAAAGAAGTAGACAACCAATTTCATTGGACAGAGCTATCGCTGATCAAAGCAACTTAATACTGAAGGAGATTATACCAGGATCTGAAACGATTCCGGAGAAGATGGTTGAGAGGCAGTTAGTGACGCAAGGGGTTGTGAATCTTCTTAACACACTTAACAAGAGAGAAGAGATAGTAAGATTATACTTTGGACTCAATGGAGAGAATCTGACAGATTCATGGCATTGCATTGTCAAAATTGCAAACAAATTCACTTGTAGATAG

Protein sequence

>Msa0495830

MVAKIAEANNVLRRRLRRKPTYNEIAEVLNVNVSTVKLVSERSRQPISLDRAIADQSNLILKEIIPGSETIPEKMVERQLVTQGVVNLLNTLNKREEIVRLYFGLNGENLTDSWHCIVKIANKFTCR*