Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139410 | XP_003607297.1 | 97.059 | 68 | 2 | 0 | 1 | 68 | 1 | 68 | 1.49e-40 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139410 | G7JPM7 | 97.059 | 68 | 2 | 0 | 1 | 68 | 1 | 68 | 7.14e-41 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0012440 | Msa1139410 | 0.832789 | 7.736645e-56 | -8.615850e-47 |
| Msa0022640 | Msa1139410 | 0.835594 | 1.532068e-56 | -8.615850e-47 |
| Msa0032500 | Msa1139410 | 0.805937 | 1.048675e-49 | -8.615850e-47 |
| Msa0033340 | Msa1139410 | 0.804824 | 1.794725e-49 | -8.615850e-47 |
| Msa0036430 | Msa1139410 | 0.804202 | 2.419374e-49 | -8.615850e-47 |
| Msa0057240 | Msa1139410 | 0.820137 | 8.040718e-53 | -8.615850e-47 |
| Msa0118400 | Msa1139410 | 0.810008 | 1.426661e-50 | -8.615850e-47 |
| Msa0607200 | Msa1139410 | 0.802031 | 6.803588e-49 | -8.615850e-47 |
| Msa0610370 | Msa1139410 | 0.802952 | 4.395101e-49 | -8.615850e-47 |
| Msa0611380 | Msa1139410 | 0.807011 | 6.226384e-50 | -8.615850e-47 |
| Msa0618290 | Msa1139410 | 0.803462 | 3.445940e-49 | -8.615850e-47 |
| Msa0631120 | Msa1139410 | 0.803514 | 3.361322e-49 | -8.615850e-47 |
| Msa0641900 | Msa1139410 | 0.821409 | 4.100609e-53 | -8.615850e-47 |
| Msa0649970 | Msa1139410 | 0.831035 | 2.097464e-55 | -8.615850e-47 |
| Msa0650900 | Msa1139410 | 0.808878 | 2.494853e-50 | -8.615850e-47 |
| Msa0661870 | Msa1139410 | 0.802042 | 6.767654e-49 | -8.615850e-47 |
| Msa0663090 | Msa1139410 | 0.802112 | 6.548436e-49 | -8.615850e-47 |
| Msa0666030 | Msa1139410 | 0.829747 | 4.330640e-55 | -8.615850e-47 |
| Msa0716450 | Msa1139410 | 0.810059 | 1.391026e-50 | -8.615850e-47 |
| Msa0717260 | Msa1139410 | 0.814533 | 1.467556e-51 | -8.615850e-47 |
| Msa0734660 | Msa1139410 | 0.808737 | 2.673233e-50 | -8.615850e-47 |
| Msa0758320 | Msa1139410 | 0.812730 | 3.660667e-51 | -8.615850e-47 |
| Msa0776220 | Msa1139410 | 0.805680 | 1.187371e-49 | -8.615850e-47 |
| Msa0837980 | Msa1139410 | 0.805601 | 1.233663e-49 | -8.615850e-47 |
| Msa0130570 | Msa1139410 | 0.805337 | 1.401490e-49 | -8.615850e-47 |
| Msa0149560 | Msa1139410 | 0.824061 | 9.895345e-54 | -8.615850e-47 |
| Msa0161680 | Msa1139410 | 0.833845 | 4.220472e-56 | -8.615850e-47 |
| Msa0203030 | Msa1139410 | 0.819992 | 8.678674e-53 | -8.615850e-47 |
| Msa0213080 | Msa1139410 | 0.807932 | 3.969301e-50 | -8.615850e-47 |
| Msa0219190 | Msa1139410 | 0.814195 | 1.743823e-51 | -8.615850e-47 |
| Msa0235190 | Msa1139410 | 0.805486 | 1.304494e-49 | -8.615850e-47 |
| Msa0371540 | Msa1139410 | 0.807041 | 6.134283e-50 | -8.615850e-47 |
| Msa0408630 | Msa1139410 | 0.819925 | 8.988241e-53 | -8.615850e-47 |
| Msa0419820 | Msa1139410 | 0.813077 | 3.072413e-51 | -8.615850e-47 |
| Msa0419970 | Msa1139410 | 0.807945 | 3.945124e-50 | -8.615850e-47 |
| Msa0436460 | Msa1139410 | 0.828848 | 7.156096e-55 | -8.615850e-47 |
| Msa0452410 | Msa1139410 | 0.815687 | 8.138600e-52 | -8.615850e-47 |
| Msa1019020 | Msa1139410 | 0.800309 | 1.531087e-48 | -8.615850e-47 |
| Msa1020720 | Msa1139410 | 0.979971 | 3.742010e-149 | -8.615850e-47 |
| Msa1027240 | Msa1139410 | 0.809997 | 1.434682e-50 | -8.615850e-47 |
| Msa1033560 | Msa1139410 | 0.814512 | 1.483354e-51 | -8.615850e-47 |
| Msa1034680 | Msa1139410 | 0.815835 | 7.541823e-52 | -8.615850e-47 |
| Msa1060530 | Msa1139410 | 0.805034 | 1.621663e-49 | -8.615850e-47 |
| Msa1104000 | Msa1139410 | 0.804145 | 2.486181e-49 | -8.615850e-47 |
| Msa0251240 | Msa1139410 | 0.809591 | 1.754230e-50 | -8.615850e-47 |
| Msa0257680 | Msa1139410 | 0.807221 | 5.619656e-50 | -8.615850e-47 |
| Msa0273570 | Msa1139410 | 0.821382 | 4.159522e-53 | -8.615850e-47 |
| Msa0313920 | Msa1139410 | 0.806641 | 7.452451e-50 | -8.615850e-47 |
| Msa0322830 | Msa1139410 | 0.802789 | 4.748353e-49 | -8.615850e-47 |
| Msa0322840 | Msa1139410 | 0.808740 | 2.670121e-50 | -8.615850e-47 |
| Msa0335270 | Msa1139410 | 0.801030 | 1.091056e-48 | -8.615850e-47 |
| Msa1113850 | Msa1139410 | 0.807038 | 6.143803e-50 | -8.615850e-47 |
| Msa1139410 | Msa1147570 | 0.803333 | 3.665197e-49 | -8.615850e-47 |
| Msa1139410 | Msa1160400 | 0.804554 | 2.043152e-49 | -8.615850e-47 |
| Msa1139410 | Msa1218480 | 0.823742 | 1.175832e-53 | -8.615850e-47 |
| Msa1139410 | Msa1275770 | 0.801433 | 9.024231e-49 | -8.615850e-47 |
| Msa1139410 | Msa1287560 | 0.813791 | 2.140827e-51 | -8.615850e-47 |
| Msa1139410 | Msa1288000 | 0.802006 | 6.884344e-49 | -8.615850e-47 |
| Msa1139410 | Msa1289280 | 0.800412 | 1.458888e-48 | -8.615850e-47 |
| Msa1139410 | Msa1324780 | 0.828514 | 8.620322e-55 | -8.615850e-47 |
| Msa1139410 | Msa1359820 | 0.804942 | 1.695561e-49 | -8.615850e-47 |
| Msa1139410 | Msa1374440 | 0.832476 | 9.249451e-56 | -8.615850e-47 |
| Msa1139410 | Msa1379990 | 0.840696 | 7.441921e-58 | -8.615850e-47 |
| Msa1139410 | Msa1419010 | 0.811110 | 8.245651e-51 | -8.615850e-47 |
| Msa1139410 | Msa1420060 | 0.828755 | 7.534869e-55 | -8.615850e-47 |
| Msa1139410 | Msa1422270 | 0.820957 | 5.212989e-53 | -8.615850e-47 |
| Msa1139410 | Msa1423990 | 0.833937 | 4.002102e-56 | -8.615850e-47 |
| Msa1139410 | Msa1429420 | 0.826819 | 2.200666e-54 | -8.615850e-47 |
| Msa1139410 | Msa1440340 | 0.823005 | 1.747879e-53 | -8.615850e-47 |
| Msa1139410 | Msa1453710 | 0.823738 | 1.178128e-53 | -8.615850e-47 |
| Msa0484690 | Msa1139410 | 0.830833 | 2.350510e-55 | -8.615850e-47 |
| Msa0495830 | Msa1139410 | 0.801579 | 8.425087e-49 | -8.615850e-47 |
| Msa0496000 | Msa1139410 | 0.811471 | 6.887773e-51 | -8.615850e-47 |
| Msa0517340 | Msa1139410 | 0.803995 | 2.670733e-49 | -8.615850e-47 |
| Msa0534590 | Msa1139410 | 0.818750 | 1.665667e-52 | -8.615850e-47 |
| Msa0555950 | Msa1139410 | 0.810989 | 8.760300e-51 | -8.615850e-47 |
| Msa0584130 | Msa1139410 | 0.853347 | 2.538352e-61 | -8.615850e-47 |
| Msa0590800 | Msa1139410 | 0.805071 | 1.593651e-49 | -8.615850e-47 |
| Msa0870620 | Msa1139410 | 0.818833 | 1.594680e-52 | -8.615850e-47 |
| Msa0880140 | Msa1139410 | 0.800384 | 1.478345e-48 | -8.615850e-47 |
| Msa0880710 | Msa1139410 | 0.810913 | 9.100891e-51 | -8.615850e-47 |
| Msa0884920 | Msa1139410 | 0.801535 | 8.599215e-49 | -8.615850e-47 |
| Msa0898370 | Msa1139410 | 0.811717 | 6.087756e-51 | -8.615850e-47 |
| Msa0936270 | Msa1139410 | 0.836695 | 8.047666e-57 | -8.615850e-47 |
| Msa0946320 | Msa1139410 | 0.825686 | 4.094414e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139410 | MtrunA17_Chr4g0038821 | 97.059 | 68 | 2 | 0 | 1 | 68 | 1 | 68 | 1.37e-44 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1139410 | AT3G22210.1 | 62.500 | 64 | 23 | 1 | 5 | 68 | 6 | 68 | 1.55e-20 | 77.0 |
Find 23 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGAACATGCAACTATTT+TGG | 0.167660 | 7_4:+9652594 | None:intergenic |
| CAATTCGCTCTTCCAGTATT+GGG | 0.341545 | 7_4:-9653158 | Msa1139410:CDS |
| GTCTTCTAGCTCGCCTCTCT+CGG | 0.346161 | 7_4:+9652925 | None:intergenic |
| GACAGAAATCACTTCAAGAT+TGG | 0.349085 | 7_4:-9652995 | Msa1139410:intron |
| GCCGCAACAATGCCCAATAC+TGG | 0.366936 | 7_4:+9653146 | None:intergenic |
| AGCTCGCCTCTCTCGGGAGC+TGG | 0.374844 | 7_4:+9652932 | None:intergenic |
| GCAATTCGCTCTTCCAGTAT+TGG | 0.399436 | 7_4:-9653159 | Msa1139410:CDS |
| CTCTCTCGGGAGCTGGATGT+TGG | 0.452110 | 7_4:+9652939 | None:intergenic |
| AGTAGCAAATTGTTTGTACT+AGG | 0.459932 | 7_4:-9652790 | Msa1139410:three_prime_UTR |
| TCCAGTATTGGGCATTGTTG+CGG | 0.480204 | 7_4:-9653147 | Msa1139410:CDS |
| ACAGAAATCACTTCAAGATT+GGG | 0.482353 | 7_4:-9652994 | Msa1139410:intron |
| ATTGAAACTCACTGCATAGA+AGG | 0.485260 | 7_4:+9653112 | None:intergenic |
| TCTTCTAGCTCGCCTCTCTC+GGG | 0.500761 | 7_4:+9652926 | None:intergenic |
| CGAGGGAGAAAAGTCATTCA+AGG | 0.507786 | 7_4:+9652560 | None:intergenic |
| ACTAACAAGAACTATTGAAA+AGG | 0.512776 | 7_4:+9652759 | None:intergenic |
| GATGATTCTGAATCAGAGAA+TGG | 0.538128 | 7_4:-9652972 | Msa1139410:CDS |
| TCCAATAGAAAATGGAACAC+GGG | 0.570237 | 7_4:+9652721 | None:intergenic |
| CAACATCCAGCTCCCGAGAG+AGG | 0.612999 | 7_4:-9652938 | Msa1139410:CDS |
| TTCCAATAGAAAATGGAACA+CGG | 0.614078 | 7_4:+9652720 | None:intergenic |
| GAGTTTCAATGAGATTAGAG+AGG | 0.641117 | 7_4:-9653099 | Msa1139410:intron |
| GATTCTGAATCAGAGAATGG+AGG | 0.645594 | 7_4:-9652969 | Msa1139410:CDS |
| ACAACAAGTTGCACATCCAG+AGG | 0.652049 | 7_4:-9652617 | Msa1139410:three_prime_UTR |
| GTGAGACTAGTCACTGACCC+AGG | 0.670217 | 7_4:+9652638 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTGTCATAATTCAATTTTAT+AGG | + | chr7_4:9652726-9652745 | None:intergenic | 20.0% |
| !!! | ATTTCTTAATTTTTGATGCT+TGG | - | chr7_4:9652906-9652925 | Msa1139410:CDS | 20.0% |
| !!! | TACATTTTTCCAATAGAAAA+TGG | + | chr7_4:9653026-9653045 | None:intergenic | 20.0% |
| !!! | CATTTGTCGAAGTTATTTTT+TGG | + | chr7_4:9652852-9652871 | None:intergenic | 25.0% |
| ! | ACTAACAAGAACTATTGAAA+AGG | + | chr7_4:9652980-9652999 | None:intergenic | 25.0% |
| !! | AAATGAACATGCAACTATTT+TGG | + | chr7_4:9653145-9653164 | None:intergenic | 25.0% |
| ACAGAAATCACTTCAAGATT+GGG | - | chr7_4:9652742-9652761 | Msa1139410:three_prime_UTR | 30.0% | |
| AGTAGCAAATTGTTTGTACT+AGG | - | chr7_4:9652946-9652965 | Msa1139410:CDS | 30.0% | |
| TTCCAATAGAAAATGGAACA+CGG | + | chr7_4:9653019-9653038 | None:intergenic | 30.0% | |
| !!! | ACATTTTTTGTGTTTTCTCC+TGG | - | chr7_4:9653080-9653099 | Msa1139410:intron | 30.0% |
| !!! | CATTTTTTGTGTTTTCTCCT+GGG | - | chr7_4:9653081-9653100 | Msa1139410:intron | 30.0% |
| ATTGAAACTCACTGCATAGA+AGG | + | chr7_4:9652627-9652646 | None:intergenic | 35.0% | |
| GAGTTTCAATGAGATTAGAG+AGG | - | chr7_4:9652637-9652656 | Msa1139410:three_prime_UTR | 35.0% | |
| GACAGAAATCACTTCAAGAT+TGG | - | chr7_4:9652741-9652760 | Msa1139410:three_prime_UTR | 35.0% | |
| GATGATTCTGAATCAGAGAA+TGG | - | chr7_4:9652764-9652783 | Msa1139410:three_prime_UTR | 35.0% | |
| TCCAATAGAAAATGGAACAC+GGG | + | chr7_4:9653018-9653037 | None:intergenic | 35.0% | |
| CAATTCGCTCTTCCAGTATT+GGG | - | chr7_4:9652578-9652597 | Msa1139410:three_prime_UTR | 40.0% | |
| GATTCTGAATCAGAGAATGG+AGG | - | chr7_4:9652767-9652786 | Msa1139410:three_prime_UTR | 40.0% | |
| GCAATTCGCTCTTCCAGTAT+TGG | - | chr7_4:9652577-9652596 | Msa1139410:three_prime_UTR | 45.0% | |
| ! | TCCAGTATTGGGCATTGTTG+CGG | - | chr7_4:9652589-9652608 | Msa1139410:three_prime_UTR | 45.0% |
| ! | GCCCGTGTTCCATTTTCTAT+TGG | - | chr7_4:9653014-9653033 | Msa1139410:intron | 45.0% |
| ACAACAAGTTGCACATCCAG+AGG | - | chr7_4:9653119-9653138 | Msa1139410:CDS | 45.0% | |
| !!! | CATGCAACTATTTTGGCCTC+TGG | + | chr7_4:9653138-9653157 | None:intergenic | 45.0% |
| GCCGCAACAATGCCCAATAC+TGG | + | chr7_4:9652593-9652612 | None:intergenic | 55.0% | |
| !! | TCTTCTAGCTCGCCTCTCTC+GGG | + | chr7_4:9652813-9652832 | None:intergenic | 55.0% |
| !! | GTCTTCTAGCTCGCCTCTCT+CGG | + | chr7_4:9652814-9652833 | None:intergenic | 55.0% |
| ! | GTGAGACTAGTCACTGACCC+AGG | + | chr7_4:9653101-9653120 | None:intergenic | 55.0% |
| CTCTCTCGGGAGCTGGATGT+TGG | + | chr7_4:9652800-9652819 | None:intergenic | 60.0% | |
| CAACATCCAGCTCCCGAGAG+AGG | - | chr7_4:9652798-9652817 | Msa1139410:three_prime_UTR | 60.0% | |
| AGCTCGCCTCTCTCGGGAGC+TGG | + | chr7_4:9652807-9652826 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 9652566 | 9653192 | 9652566 | ID=Msa1139410;Name=Msa1139410 |
| chr7_4 | mRNA | 9652566 | 9653192 | 9652566 | ID=Msa1139410-mRNA-1;Parent=Msa1139410;Name=Msa1139410-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|1|0.5|1|1|1|2|333|68 |
| chr7_4 | exon | 9652566 | 9653012 | 9652566 | ID=Msa1139410-mRNA-1:exon:2476;Parent=Msa1139410-mRNA-1 |
| chr7_4 | exon | 9653100 | 9653192 | 9653100 | ID=Msa1139410-mRNA-1:exon:2475;Parent=Msa1139410-mRNA-1 |
| chr7_4 | CDS | 9653100 | 9653192 | 9653100 | ID=Msa1139410-mRNA-1:cds;Parent=Msa1139410-mRNA-1 |
| chr7_4 | CDS | 9652899 | 9653012 | 9652899 | ID=Msa1139410-mRNA-1:cds;Parent=Msa1139410-mRNA-1 |
| chr7_4 | three_prime_UTR | 9652566 | 9652898 | 9652566 | ID=Msa1139410-mRNA-1:three_prime_utr;Parent=Msa1139410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
