Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0898370 | RHN52769.1 | 85.768 | 267 | 35 | 1 | 1 | 264 | 1 | 267 | 7.90e-157 | 447 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0898370 | sp|Q8VYG0|LRL13_ARATH | 31.760 | 233 | 129 | 10 | 3 | 225 | 12 | 224 | 9.29e-20 | 92.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0898370 | A0A396HHG2 | 85.768 | 267 | 35 | 1 | 1 | 264 | 1 | 267 | 3.78e-157 | 447 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0007010 | Msa0898370 | 0.812422 | 4.274354e-51 | -8.615850e-47 |
| Msa0011720 | Msa0898370 | 0.810971 | 8.839786e-51 | -8.615850e-47 |
| Msa0022640 | Msa0898370 | 0.808944 | 2.414180e-50 | -8.615850e-47 |
| Msa0032500 | Msa0898370 | 0.808037 | 3.770670e-50 | -8.615850e-47 |
| Msa0033340 | Msa0898370 | 0.810778 | 9.731891e-51 | -8.615850e-47 |
| Msa0044710 | Msa0898370 | 0.806253 | 8.999390e-50 | -8.615850e-47 |
| Msa0055730 | Msa0898370 | 0.806984 | 6.308896e-50 | -8.615850e-47 |
| Msa0091770 | Msa0898370 | 0.804369 | 2.232544e-49 | -8.615850e-47 |
| Msa0134220 | Msa0898370 | 0.812306 | 4.530221e-51 | -8.615850e-47 |
| Msa0149170 | Msa0898370 | 0.819164 | 1.340801e-52 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0898370 | MtrunA17_Chr6g0484061 | 85.768 | 267 | 35 | 1 | 1 | 264 | 1 | 267 | 7.27e-161 | 447 |
| Msa0898370 | MtrunA17_Chr6g0467631 | 57.471 | 261 | 83 | 7 | 5 | 246 | 11 | 262 | 2.58e-87 | 273 |
| Msa0898370 | MtrunA17_Chr6g0484051 | 52.529 | 257 | 95 | 7 | 23 | 256 | 23 | 275 | 3.04e-75 | 241 |
| Msa0898370 | MtrunA17_Chr6g0483931 | 51.321 | 265 | 104 | 8 | 4 | 246 | 10 | 271 | 3.19e-74 | 239 |
| Msa0898370 | MtrunA17_Chr6g0483821 | 51.471 | 272 | 104 | 8 | 1 | 249 | 7 | 273 | 3.85e-72 | 233 |
| Msa0898370 | MtrunA17_Chr6g0483901 | 49.333 | 225 | 91 | 6 | 4 | 212 | 10 | 227 | 3.05e-60 | 191 |
| Msa0898370 | MtrunA17_Chr8g0376061 | 35.102 | 245 | 129 | 10 | 32 | 258 | 24 | 256 | 4.28e-28 | 112 |
| Msa0898370 | MtrunA17_Chr8g0391231 | 32.721 | 272 | 147 | 10 | 8 | 256 | 1 | 259 | 2.53e-27 | 110 |
| Msa0898370 | MtrunA17_Chr6g0484081 | 55.056 | 89 | 33 | 3 | 27 | 110 | 26 | 112 | 3.54e-22 | 88.6 |
| Msa0898370 | MtrunA17_Chr6g0467591 | 56.338 | 71 | 26 | 1 | 5 | 75 | 30 | 95 | 7.46e-21 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0898370 | AT5G38210.1 | 31.760 | 233 | 129 | 10 | 3 | 225 | 12 | 224 | 9.44e-21 | 92.0 |
| Msa0898370 | AT1G67000.1 | 32.444 | 225 | 124 | 11 | 44 | 256 | 38 | 246 | 8.20e-19 | 86.3 |
| Msa0898370 | AT1G66940.2 | 32.796 | 186 | 116 | 7 | 44 | 224 | 37 | 218 | 2.84e-18 | 83.2 |
| Msa0898370 | AT1G66940.3 | 32.796 | 186 | 116 | 7 | 44 | 224 | 37 | 218 | 2.84e-18 | 83.2 |
| Msa0898370 | AT1G66940.1 | 32.796 | 186 | 116 | 7 | 44 | 224 | 37 | 218 | 3.13e-18 | 83.2 |
| Msa0898370 | AT1G66940.4 | 32.796 | 186 | 116 | 7 | 44 | 224 | 37 | 218 | 4.85e-18 | 82.8 |
| Msa0898370 | AT1G66880.1 | 32.663 | 199 | 99 | 9 | 43 | 224 | 23 | 203 | 1.67e-17 | 82.4 |
| Msa0898370 | AT1G66880.1 | 28.704 | 216 | 121 | 9 | 22 | 224 | 400 | 595 | 1.04e-13 | 71.2 |
| Msa0898370 | AT1G66930.2 | 31.980 | 197 | 103 | 10 | 44 | 225 | 37 | 217 | 3.36e-15 | 75.5 |
| Msa0898370 | AT1G66930.2 | 26.766 | 269 | 142 | 13 | 15 | 250 | 231 | 477 | 8.67e-12 | 65.5 |
Find 47 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATATTGAAGTGCGCGT+AGG | 0.643061 | 6_1:-91935191 | Msa0898370:CDS |
| AAAGAGATATATGTAATGTT+TGG | 0.298188 | 6_1:+91935728 | None:intergenic |
| AACATCGAATATCCATTCTG+GGG | 0.702136 | 6_1:-91935584 | Msa0898370:CDS |
| AAGAAAGTATCATTGTATTG+AGG | 0.582725 | 6_1:+91935389 | Msa0898360:intron |
| AAGAGATATATGTAATGTTT+GGG | 0.405412 | 6_1:+91935729 | None:intergenic |
| AATCGTGAACTGGTTGAGAC+TGG | 0.453887 | 6_1:+91935657 | None:intergenic |
| ACATTGGAATAAGAAAAGGA+AGG | 0.711357 | 6_1:+91935251 | Msa0898360:intron |
| ATATTGTTCGAGTGATTATT+TGG | 0.242766 | 6_1:-91935069 | Msa0898370:CDS |
| ATCGTGAACTGGTTGAGACT+GGG | 0.573683 | 6_1:+91935658 | None:intergenic |
| ATGTCTATGATGTGGACACT+TGG | 0.571414 | 6_1:+91935341 | Msa0898360:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGTAGTATATGTCTATGATG+TGG | + | chr6_1:91935366-91935385 | Msa0898360:intron | 30.0% | |
| ACATTGGAATAAGAAAAGGA+AGG | + | chr6_1:91935448-91935467 | Msa0898360:intron | 30.0% | |
| TGAGGATCTTGAAAATGATT+TGG | - | chr6_1:91935540-91935559 | Msa0898370:CDS | 30.0% | |
| TGAGGGATGTTTAAATTGTT+TGG | - | chr6_1:91935594-91935613 | Msa0898370:CDS | 30.0% | |
| GAGGGATGTTTAAATTGTTT+GGG | - | chr6_1:91935595-91935614 | Msa0898370:CDS | 30.0% | |
| ATTATGATAATTGTCCAGAC+GGG | - | chr6_1:91935671-91935690 | Msa0898370:CDS | 30.0% | |
| GAAAACATACTATTGTGAAG+AGG | + | chr6_1:91935718-91935737 | None:intergenic | 30.0% | |
| TGTAGTATATGTCTATGATG+TGG | + | chr6_1:91935366-91935385 | Msa0898360:intron | 30.0% | |
| ACATTGGAATAAGAAAAGGA+AGG | + | chr6_1:91935448-91935467 | Msa0898360:intron | 30.0% | |
| TGAGGATCTTGAAAATGATT+TGG | - | chr6_1:91935540-91935559 | Msa0898370:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 91934962 | 91935756 | 91934962 | ID=Msa0898370;Name=Msa0898370 |
| chr6_1 | mRNA | 91934962 | 91935756 | 91934962 | ID=Msa0898370-mRNA-1;Parent=Msa0898370;Name=Msa0898370-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|264 |
| chr6_1 | exon | 91934962 | 91935756 | 91934962 | ID=Msa0898370-mRNA-1:exon:15591;Parent=Msa0898370-mRNA-1 |
| chr6_1 | CDS | 91934962 | 91935756 | 91934962 | ID=Msa0898370-mRNA-1:cds;Parent=Msa0898370-mRNA-1 |
| Gene Sequence |
>Msa0898370 ATGTTCCCAAACATTACATATATCTCTTTGTTATTTCACTTCTTATCGATAATAATCACTTCTTTTCCAACACAATCCCAGTCTCAACCAGTTCACGATTATTCAAGATGTAATGATGTCGACCATTCCTACAACTGCGGAAATATTTCCAACATCGAATATCCATTCTGGGGACAAAATCGTCCGTTTAACTGCGGCGCGGGCAACCCATTTTACCTTAACTGCCACAGCAACAGTATTACTACCATTCTATTATCATCACAAAATTTCACAGTACTTGATATTAACGCACAAGATCACACCATCAAACTCAAGAGAACAGACCTTTCCCAAAATTTTTGTTCTCCTCAATACAATGATACTTTCTTGTCTCCAAAAATATTTCAGTACCTCCCAAGTGTCCACATCATAGACATATACTACAATTGTACTTACAATGTCTCTCAAAATTTACTTAATAAATCTCTGTGTGGATCTCACAATCCTTCCTTTTCTTATTCCAATGTGCCGTATGACGAAAATCTAGATCAGAAGAGATGTGAGAAACATATTGAAGTGCGCGTAGGAGAAGATAATTCTGAGGATCTTGAAAATGATTTGGACAAGGGATTTGAGGTGGAATACAGTGTGAATGAGGGATGTTTAAATTGTTTGGGAAATGAAGGATATTGTTCGAGTGATTATTTGGATAAAAATGTTGATTTGTGCTATTATGATAATTGTCCAGACGGGTCTATTGATAATAACTGTTCTCCTCTTCACAATAGTATGTTTTCTTATCTCTTTATCTCATAA |
| Protein sequence |
>Msa0898370 MFPNITYISLLFHFLSIIITSFPTQSQSQPVHDYSRCNDVDHSYNCGNISNIEYPFWGQNRPFNCGAGNPFYLNCHSNSITTILLSSQNFTVLDINAQDHTIKLKRTDLSQNFCSPQYNDTFLSPKIFQYLPSVHIIDIYYNCTYNVSQNLLNKSLCGSHNPSFSYSNVPYDENLDQKRCEKHIEVRVGEDNSEDLENDLDKGFEVEYSVNEGCLNCLGNEGYCSSDYLDKNVDLCYYDNCPDGSIDNNCSPLHNSMFSYLFIS* |
