AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0517770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517770 MtrunA17_Chr3g0129631 89.073 302 2 1 9 279 131 432 0.0 553
Msa0517770 MtrunA17_Chr4g0057811 85.099 302 14 1 9 279 130 431 0.0 532
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517770 AT3G27740.1 69.759 291 57 1 9 268 140 430 2.63e-154 437
Msa0517770 AT3G27740.2 77.419 217 49 0 52 268 142 358 6.95e-131 375
Msa0517770 AT1G25220.1 28.571 189 122 5 91 267 86 273 5.44e-13 67.8
Msa0517770 AT1G25220.2 28.877 187 120 5 93 267 101 286 1.41e-12 67.0
Msa0517770 AT1G24909.1 28.042 189 123 5 91 267 32 219 2.43e-12 65.5
Msa0517770 AT1G25083.1 28.042 189 123 5 91 267 32 219 2.43e-12 65.5
Msa0517770 AT1G25155.1 28.042 189 123 5 91 267 32 219 2.43e-12 65.5
Msa0517770 AT5G57890.1 27.513 189 124 5 91 267 83 270 2.80e-12 65.9
Msa0517770 AT1G24807.1 28.342 187 121 5 93 267 47 232 7.72e-12 63.9

Find 97 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGGAAGATGGCAGCCTTAT+TGG 0.173503 3_4:-69491926 Msa0517770:intron
TTCTTAGTAGTTTCTATCTA+TGG 0.264325 3_4:-69489976 Msa0517770:three_prime_UTR
GCTAGCCCTTGTACAGAAAA+TGG 0.293625 3_4:+69489903 None:intergenic
ACAACCTACAAGATGAAATT+TGG 0.299468 3_4:-69491111 Msa0517770:CDS
CTATGGTTTGTAGATGAATA+AGG 0.315269 3_4:-69489959 Msa0517770:three_prime_UTR
TGGATTTAACTTCAAAGTTT+CGG 0.329533 3_4:+69491275 None:intergenic
CTGGAGATGTGTGAAGGATT+TGG 0.330063 3_4:-69492113 Msa0517770:CDS
TAGGTAGTTGCATACGATTT+TGG 0.336986 3_4:-69491375 Msa0517770:CDS
CAATTGCTAGGCCAGGCTTT+AGG 0.351638 3_4:-69491138 Msa0517770:CDS
TGAGGTCCAGGAGATGCTTC+AGG 0.357210 3_4:+69490386 None:intergenic
AACTTCAAAGTTTCGGATGC+TGG 0.363527 3_4:+69491282 None:intergenic
GTGACCAAATTTCATCTTGT+AGG 0.368330 3_4:+69491107 None:intergenic
TCATCCAATAGGTTTGGATT+GGG 0.377058 3_4:+69489696 None:intergenic
GCACAATGTCTTCATCCAAT+AGG 0.380576 3_4:+69489685 None:intergenic
AATCCCCAATCCAAACCTAT+TGG 0.381071 3_4:-69489700 Msa0517770:three_prime_UTR
GTTCTATCAATCCATTCATA+TGG 0.384320 3_4:+69491539 None:intergenic
ACTCCTTCAGGCAGTGTGGC+AGG 0.385852 3_4:+69490494 None:intergenic
TGCTATCCCACCAGGCTAGA+AGG 0.386176 3_4:-69490032 Msa0517770:three_prime_UTR
GGGCAATCACACGTAGATTA+CGG 0.396622 3_4:-69491946 Msa0517770:CDS
ATGTCTTCATCCAATAGGTT+TGG 0.401382 3_4:+69489690 None:intergenic
TCGGATGCTGGCCATGTAGA+TGG 0.404003 3_4:+69491294 None:intergenic
TGAGTAACTTCTACTCCTTC+AGG 0.404731 3_4:+69490482 None:intergenic
TTTACTGTTTCAACGGCATA+AGG 0.405190 3_4:+69491207 None:intergenic
GGCAATCACACGTAGATTAC+GGG 0.418709 3_4:-69491945 Msa0517770:CDS
TGGAGATGTGTGAAGGATTT+GGG 0.420135 3_4:-69492112 Msa0517770:CDS
TTCATCCAATAGGTTTGGAT+TGG 0.422242 3_4:+69489695 None:intergenic
TTGATAGAACAAAGGAAGAA+TGG 0.426377 3_4:-69491526 Msa0517770:CDS
AGCGATTAGCTAATAAAGTA+AGG 0.433096 3_4:+69489726 None:intergenic
ATTTATTCAGTACCTCAAAC+TGG 0.433137 3_4:-69492132 Msa0517770:CDS
CAGTGCTCAAAACACCAATA+AGG 0.434979 3_4:+69491912 None:intergenic
CTTCACACATCTCCAGTTTG+AGG 0.444880 3_4:+69492120 None:intergenic
CTCTTCCTGTCTCTTTGCGA+TGG 0.451577 3_4:-69492157 None:intergenic
CCGTGTTGAAATTAGTTCTC+AGG 0.452192 3_4:-69491046 Msa0517770:intron
AATACTTTATCCTTCTAGCC+TGG 0.459597 3_4:+69490022 None:intergenic
CTTAGGCGTCTAGCATCATA+TGG 0.460279 3_4:-69491336 Msa0517770:CDS
GAAACAGTAAAGAATATCAT+TGG 0.466180 3_4:-69491195 Msa0517770:CDS
ACATTATCCTTCTTCAATGA+AGG 0.472185 3_4:+69489857 None:intergenic
TACCACCCTGAAGCATCTCC+TGG 0.473772 3_4:-69490392 Msa0517770:CDS
TGATAGAACAAAGGAAGAAT+GGG 0.475190 3_4:-69491525 Msa0517770:CDS
TTGCTGAAAAGTACACCATC+TGG 0.479305 3_4:+69491255 None:intergenic
CGTGCAAGTCACCATATGAA+TGG 0.483758 3_4:-69491550 Msa0517770:CDS
GGAACAGCAGAAGGATCTCC+AGG 0.484183 3_4:+69491228 None:intergenic
AAAGTATGTGATAAAAGCTC+AGG 0.494537 3_4:-69489999 Msa0517770:three_prime_UTR
TAGATGAATAAGGACTTCCT+TGG 0.495299 3_4:-69489949 Msa0517770:three_prime_UTR
AATATCCATCGCAAAGAGAC+AGG 0.495642 3_4:+69492152 None:intergenic
GATATTCTTTACTGTTTCAA+CGG 0.495805 3_4:+69491200 None:intergenic
TTGAGCAGGGAAAGCAAGAC+CGG 0.499137 3_4:+69490433 None:intergenic
CTCCTTCAGGCAGTGTGGCA+GGG 0.505914 3_4:+69490495 None:intergenic
GAATCAAGCATAATATACTT+AGG 0.506239 3_4:-69491353 Msa0517770:CDS
CTCGTAGCCTTCATTGAAGA+AGG 0.515567 3_4:-69489864 Msa0517770:three_prime_UTR
CCAGTTCGAAGGTTACGAAC+AGG 0.516140 3_4:+69491069 None:intergenic
CTCAATGACAGTAGTTGTGC+CGG 0.517106 3_4:-69490452 Msa0517770:CDS
CTTAACTGAAAGGAATGTCA+TGG 0.522571 3_4:-69492083 Msa0517770:CDS
TTGTCAGAATCATGAGGTCC+AGG 0.525671 3_4:+69490374 None:intergenic
GGGACATCAATTGCTAGGCC+AGG 0.526918 3_4:-69491145 Msa0517770:CDS
GAGGTCCAGGAGATGCTTCA+GGG 0.527220 3_4:+69490387 None:intergenic
AGTATCTCTAGGCCCTTCAC+TGG 0.527684 3_4:+69491493 None:intergenic
CTTTATCCTTCTAGCCTGGT+GGG 0.529670 3_4:+69490026 None:intergenic
CCTGTTCGTAACCTTCGAAC+TGG 0.534549 3_4:-69491069 Msa0517770:CDS
CATCCAATAGGTTTGGATTG+GGG 0.536858 3_4:+69489697 None:intergenic
TGGGCGATTACTTAACTGAA+AGG 0.537172 3_4:-69492093 Msa0517770:CDS
CTTACATGGAAAGTATCTCT+AGG 0.537477 3_4:+69491482 None:intergenic
AGGTTGTACCTCCTAAAGCC+TGG 0.548433 3_4:+69491127 None:intergenic
CAACAGATGATGTTGATACA+AGG 0.549921 3_4:-69491967 Msa0517770:intron
ACACTGATTGCAGTTGGTGT+AGG 0.551642 3_4:+69490074 None:intergenic
ACACGTAGATTACGGGAAGA+TGG 0.551699 3_4:-69491938 Msa0517770:CDS
GGCTAGCAGCCGATTGGCAT+AGG 0.554150 3_4:-69489887 Msa0517770:three_prime_UTR
TACAAGGGCTAGCAGCCGAT+TGG 0.556106 3_4:-69489893 Msa0517770:three_prime_UTR
TCACTGTTGTGCCATCTACA+TGG 0.561762 3_4:-69491305 Msa0517770:CDS
GAACAGGATGATTTCCTCCA+TGG 0.563497 3_4:+69491085 None:intergenic
AAGCTAGCTGCTATCCCACC+AGG 0.568493 3_4:-69490040 Msa0517770:three_prime_UTR
TTTCAACACGGCCAGTTCGA+AGG 0.571733 3_4:+69491058 None:intergenic
CAGGGTCAACTGCATAGTTG+TGG 0.573224 3_4:+69490513 None:intergenic
TGCATGGGACATCAATTGCT+AGG 0.573890 3_4:-69491150 Msa0517770:CDS
AGTAAAGAATATCATTGGAA+AGG 0.576011 3_4:-69491190 Msa0517770:CDS
GGCTACGAGCCTATGCCAAT+CGG 0.576216 3_4:+69489878 None:intergenic
TTGCTAGGCCAGGCTTTAGG+AGG 0.580876 3_4:-69491135 Msa0517770:CDS
GCGATTAGCTAATAAAGTAA+GGG 0.585873 3_4:+69489727 None:intergenic
ATTATTACACTGATTGCAGT+TGG 0.586719 3_4:+69490068 None:intergenic
GACCCTGCCACACTGCCTGA+AGG 0.593729 3_4:-69490497 Msa0517770:CDS
GAAAATCTTGATCAACTCCA+AGG 0.593981 3_4:+69489932 None:intergenic
TTAACTGAAAGGAATGTCAT+GGG 0.597258 3_4:-69492082 Msa0517770:CDS
TGGGAGTTTAGCTCCAGTGA+AGG 0.599112 3_4:-69491506 Msa0517770:CDS
ACTTTATCCTTCTAGCCTGG+TGG 0.599459 3_4:+69490025 None:intergenic
ACGGCATAAGGAACAGCAGA+AGG 0.606024 3_4:+69491219 None:intergenic
CTCAAACTGGAGATGTGTGA+AGG 0.613677 3_4:-69492119 Msa0517770:CDS
AAGATGAAATTTGGTCACCA+TGG 0.620210 3_4:-69491102 Msa0517770:CDS
ACTTTGAAGTTAAATCCAGA+TGG 0.620988 3_4:-69491270 Msa0517770:CDS
ATGAAATTTGGTCACCATGG+AGG 0.624582 3_4:-69491099 Msa0517770:CDS
TGAATGGATTGATAGAACAA+AGG 0.627214 3_4:-69491534 Msa0517770:CDS
GGGAGTTTAGCTCCAGTGAA+GGG 0.629622 3_4:-69491505 Msa0517770:CDS
AACAGATGATGTTGATACAA+GGG 0.640054 3_4:-69491966 Msa0517770:intron
GGACCTCATGATTCTGACAA+CGG 0.640994 3_4:-69490371 Msa0517770:intron
TTCTACTCCTTCAGGCAGTG+TGG 0.658466 3_4:+69490490 None:intergenic
TCACCGTTGTCAGAATCATG+AGG 0.688390 3_4:+69490368 None:intergenic
CCTGAGAACTAATTTCAACA+CGG 0.701914 3_4:+69491046 None:intergenic
GTCCAGGAGATGCTTCAGGG+TGG 0.722700 3_4:+69490390 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTGATACATATAAAT+TGG - chr3_4:69491275-69491294 Msa0517770:CDS 10.0%
!! AATAAATAAAAAATTGCAAC+TGG - chr3_4:69491022-69491041 Msa0517770:intron 15.0%
!!! ATAGCATAAAATTTTGAATA+TGG - chr3_4:69491112-69491131 Msa0517770:CDS 15.0%
!! TGATAATAAATATAACTGTT+TGG - chr3_4:69491507-69491526 Msa0517770:CDS 15.0%
!!! AAATTTTTTAAAGATTGTGA+AGG + chr3_4:69491533-69491552 None:intergenic 15.0%
!! TTAAGTAATTACCATGATTA+CGG + chr3_4:69490839-69490858 None:intergenic 20.0%
!! TAAATCCATTTGAATTCATT+TGG - chr3_4:69490856-69490875 Msa0517770:intron 20.0%
!!! ATATTTTTTTGCTTCATAGA+AGG - chr3_4:69490923-69490942 Msa0517770:intron 20.0%
!!! TATTTTTTTGCTTCATAGAA+GGG - chr3_4:69490924-69490943 Msa0517770:intron 20.0%
!! TTTAAACAAACCACAAATAT+AGG + chr3_4:69491175-69491194 None:intergenic 20.0%
!!! GTATTTTATTCTTTTTGTCA+TGG - chr3_4:69491241-69491260 Msa0517770:CDS 20.0%
!! AAAAATTTATATTCAAGAGC+AGG - chr3_4:69491545-69491564 Msa0517770:CDS 20.0%
!!! TATTTTTACAACTTGAAATG+TGG - chr3_4:69492024-69492043 Msa0517770:intron 20.0%
!!! ATTTTTACAACTTGAAATGT+GGG - chr3_4:69492025-69492044 Msa0517770:intron 20.0%
! TTACCCAAAATTCAACAAAA+GGG + chr3_4:69490388-69490407 None:intergenic 25.0%
! GAATCAAGCATAATATACTT+AGG - chr3_4:69490463-69490482 Msa0517770:CDS 25.0%
!! TGGATTTAACTTCAAAGTTT+CGG + chr3_4:69490544-69490563 None:intergenic 25.0%
! GATATTCTTTACTGTTTCAA+CGG + chr3_4:69490619-69490638 None:intergenic 25.0%
! GAAACAGTAAAGAATATCAT+TGG - chr3_4:69490621-69490640 Msa0517770:intron 25.0%
! AGTAAAGAATATCATTGGAA+AGG - chr3_4:69490626-69490645 Msa0517770:intron 25.0%
!!! TTTTATTTATTTTTGCCACG+AGG + chr3_4:69491013-69491032 None:intergenic 25.0%
!!! AAATTTTATGCTATTTCCAC+AGG + chr3_4:69491106-69491125 None:intergenic 25.0%
!! CTTGATAACATGTAATGATA+TGG + chr3_4:69491202-69491221 None:intergenic 25.0%
! ACGAAATTGTAAAAGCAAAT+TGG + chr3_4:69491603-69491622 None:intergenic 25.0%
! TTCTTAGTAGTTTCTATCTA+TGG - chr3_4:69491840-69491859 Msa0517770:intron 25.0%
!! TTTTTACAACTTGAAATGTG+GGG - chr3_4:69492026-69492045 Msa0517770:intron 25.0%
ATTTATTCAGTACCTCAAAC+TGG - chr3_4:69489684-69489703 Msa0517770:three_prime_UTR 30.0%
TTAACTGAAAGGAATGTCAT+GGG - chr3_4:69489734-69489753 Msa0517770:three_prime_UTR 30.0%
AACAGATGATGTTGATACAA+GGG - chr3_4:69489850-69489869 Msa0517770:three_prime_UTR 30.0%
TACTTCAGATGTCTAAATCA+TGG - chr3_4:69489951-69489970 Msa0517770:three_prime_UTR 30.0%
ACTTCAGATGTCTAAATCAT+GGG - chr3_4:69489952-69489971 Msa0517770:three_prime_UTR 30.0%
TCTAAATCATGGGATATTGT+TGG - chr3_4:69489962-69489981 Msa0517770:three_prime_UTR 30.0%
! TTGTTGGTAAGAATGACTAT+TGG - chr3_4:69489978-69489997 Msa0517770:three_prime_UTR 30.0%
GTTCTATCAATCCATTCATA+TGG + chr3_4:69490280-69490299 None:intergenic 30.0%
!! TGAATGGATTGATAGAACAA+AGG - chr3_4:69490282-69490301 Msa0517770:intron 30.0%
TTGATAGAACAAAGGAAGAA+TGG - chr3_4:69490290-69490309 Msa0517770:intron 30.0%
TGATAGAACAAAGGAAGAAT+GGG - chr3_4:69490291-69490310 Msa0517770:intron 30.0%
TCCATGTAAGTTTATACAGT+AGG - chr3_4:69490347-69490366 Msa0517770:intron 30.0%
! ACTCCCTTTTGTTGAATTTT+GGG - chr3_4:69490382-69490401 Msa0517770:CDS 30.0%
CTTACCCAAAATTCAACAAA+AGG + chr3_4:69490389-69490408 None:intergenic 30.0%
!! GTACTGATGAATGATCTTTT+AGG - chr3_4:69490422-69490441 Msa0517770:CDS 30.0%
!! ACTTTGAAGTTAAATCCAGA+TGG - chr3_4:69490546-69490565 Msa0517770:intron 30.0%
ACAACCTACAAGATGAAATT+TGG - chr3_4:69490705-69490724 Msa0517770:intron 30.0%
! TTGAAATTAGTTCTCAGGTA+TGG - chr3_4:69490775-69490794 Msa0517770:intron 30.0%
TCAGACCAAATGAATTCAAA+TGG + chr3_4:69490864-69490883 None:intergenic 30.0%
TTTGAATTCATTTGGTCTGA+TGG - chr3_4:69490864-69490883 Msa0517770:intron 30.0%
TTGAATTCATTTGGTCTGAT+GGG - chr3_4:69490865-69490884 Msa0517770:intron 30.0%
! TTCTTTTCTTCTCAAGACAA+GGG + chr3_4:69490962-69490981 None:intergenic 30.0%
! ATTCTTTTCTTCTCAAGACA+AGG + chr3_4:69490963-69490982 None:intergenic 30.0%
TATGTGTAGTCCTATATTTG+TGG - chr3_4:69491162-69491181 Msa0517770:CDS 30.0%
! ATTCTTTTTGTCATGGTCTT+TGG - chr3_4:69491248-69491267 Msa0517770:CDS 30.0%
GCTCTTTATCAAAAGAAGTT+TGG - chr3_4:69491482-69491501 Msa0517770:intron 30.0%
GTCTTGTAGGATATAATCAT+TGG - chr3_4:69491575-69491594 Msa0517770:intron 30.0%
ATCACAACAGTGGTTATTAT+TGG + chr3_4:69491650-69491669 None:intergenic 30.0%
ATTATCAACAATCACAACAG+TGG + chr3_4:69491660-69491679 None:intergenic 30.0%
!!! TTGTTGATAATGCAGCTTTT+CGG - chr3_4:69491669-69491688 Msa0517770:intron 30.0%
! ATTATTACACTGATTGCAGT+TGG + chr3_4:69491751-69491770 None:intergenic 30.0%
AAAGTATGTGATAAAAGCTC+AGG - chr3_4:69491817-69491836 Msa0517770:intron 30.0%
! CTATGGTTTGTAGATGAATA+AGG - chr3_4:69491857-69491876 Msa0517770:intron 30.0%
ACATTATCCTTCTTCAATGA+AGG + chr3_4:69491962-69491981 None:intergenic 30.0%
! GCGATTAGCTAATAAAGTAA+GGG + chr3_4:69492092-69492111 None:intergenic 30.0%
! AGCGATTAGCTAATAAAGTA+AGG + chr3_4:69492093-69492112 None:intergenic 30.0%
CTTAACTGAAAGGAATGTCA+TGG - chr3_4:69489733-69489752 Msa0517770:three_prime_UTR 35.0%
AGAACGTTACATGTAAACTC+TGG - chr3_4:69489792-69489811 Msa0517770:three_prime_UTR 35.0%
CAACAGATGATGTTGATACA+AGG - chr3_4:69489849-69489868 Msa0517770:three_prime_UTR 35.0%
! AAGTAATTCCTCGTCTGTTT+TGG + chr3_4:69489936-69489955 None:intergenic 35.0%
TTGGTAAGAATGACTATTGG+AGG - chr3_4:69489981-69490000 Msa0517770:three_prime_UTR 35.0%
AATTCAGTGTTGTCAAACTG+CGG - chr3_4:69490032-69490051 Msa0517770:three_prime_UTR 35.0%
! GGCTTATGTTTCTTATTTGC+AGG - chr3_4:69490220-69490239 Msa0517770:intron 35.0%
!! GCAGGTATTGATCTAATAAG+TGG - chr3_4:69490238-69490257 Msa0517770:intron 35.0%
!! CTTACATGGAAAGTATCTCT+AGG + chr3_4:69490337-69490356 None:intergenic 35.0%
GCCTACTGTATAAACTTACA+TGG + chr3_4:69490351-69490370 None:intergenic 35.0%
AAGTTTATACAGTAGGCTGT+TGG - chr3_4:69490354-69490373 Msa0517770:intron 35.0%
AATTCAACAAAAGGGAGTCA+GGG + chr3_4:69490380-69490399 None:intergenic 35.0%
AAATTCAACAAAAGGGAGTC+AGG + chr3_4:69490381-69490400 None:intergenic 35.0%
! GACTCCCTTTTGTTGAATTT+TGG - chr3_4:69490381-69490400 Msa0517770:CDS 35.0%
! TAGGTAGTTGCATACGATTT+TGG - chr3_4:69490441-69490460 Msa0517770:CDS 35.0%
TTTACTGTTTCAACGGCATA+AGG + chr3_4:69490612-69490631 None:intergenic 35.0%
!!! CCTGTTTTTGGAATTTGCAT+GGG - chr3_4:69490651-69490670 Msa0517770:intron 35.0%
GTGACCAAATTTCATCTTGT+AGG + chr3_4:69490712-69490731 None:intergenic 35.0%
AAGATGAAATTTGGTCACCA+TGG - chr3_4:69490714-69490733 Msa0517770:intron 35.0%
CCTGAGAACTAATTTCAACA+CGG + chr3_4:69490773-69490792 None:intergenic 35.0%
TCATCATTTCACCGTAATCA+TGG - chr3_4:69490825-69490844 Msa0517770:intron 35.0%
TGAATTCATTTGGTCTGATG+GGG - chr3_4:69490866-69490885 Msa0517770:intron 35.0%
TTCGTTCAACGACTTGAATT+TGG - chr3_4:69491618-69491637 Msa0517770:intron 35.0%
!!! TGTTGATAATGCAGCTTTTC+GGG - chr3_4:69491670-69491689 Msa0517770:intron 35.0%
!! AGTTGGTGTAGGTTTTCATT+CGG + chr3_4:69491734-69491753 None:intergenic 35.0%
AATACTTTATCCTTCTAGCC+TGG + chr3_4:69491797-69491816 None:intergenic 35.0%
TAGATGAATAAGGACTTCCT+TGG - chr3_4:69491867-69491886 Msa0517770:intron 35.0%
GAAAATCTTGATCAACTCCA+AGG + chr3_4:69491887-69491906 None:intergenic 35.0%
! TTGTGTCCATTTTCTGTACA+AGG - chr3_4:69491907-69491926 Msa0517770:intron 35.0%
! TGTGTCCATTTTCTGTACAA+GGG - chr3_4:69491908-69491927 Msa0517770:intron 35.0%
TAAAAACACAGTTCTCTTCG+CGG + chr3_4:69491998-69492017 None:intergenic 35.0%
TCATCCAATAGGTTTGGATT+GGG + chr3_4:69492123-69492142 None:intergenic 35.0%
TTCATCCAATAGGTTTGGAT+TGG + chr3_4:69492124-69492143 None:intergenic 35.0%
ATGTCTTCATCCAATAGGTT+TGG + chr3_4:69492129-69492148 None:intergenic 35.0%
TGGAGATGTGTGAAGGATTT+GGG - chr3_4:69489704-69489723 Msa0517770:three_prime_UTR 40.0%
TGGGCGATTACTTAACTGAA+AGG - chr3_4:69489723-69489742 Msa0517770:three_prime_UTR 40.0%
CAGTGCTCAAAACACCAATA+AGG + chr3_4:69489907-69489926 None:intergenic 40.0%
TGACAATTCCAAAACAGACG+AGG - chr3_4:69489925-69489944 Msa0517770:three_prime_UTR 40.0%
ACAACTCTGCTGGAATTACA+TGG - chr3_4:69490199-69490218 Msa0517770:intron 40.0%
AACTTCAAAGTTTCGGATGC+TGG + chr3_4:69490537-69490556 None:intergenic 40.0%
! TTGCTGAAAAGTACACCATC+TGG + chr3_4:69490564-69490583 None:intergenic 40.0%
! GATGGTGTACTTTTCAGCAA+TGG - chr3_4:69490564-69490583 Msa0517770:intron 40.0%
!!! ATTGGAAAGGTGCCTGTTTT+TGG - chr3_4:69490639-69490658 Msa0517770:intron 40.0%
!!! GCCTGTTTTTGGAATTTGCA+TGG - chr3_4:69490650-69490669 Msa0517770:intron 40.0%
CCCATGCAAATTCCAAAAAC+AGG + chr3_4:69490654-69490673 None:intergenic 40.0%
ATGAAATTTGGTCACCATGG+AGG - chr3_4:69490717-69490736 Msa0517770:intron 40.0%
! CCGTGTTGAAATTAGTTCTC+AGG - chr3_4:69490770-69490789 Msa0517770:intron 40.0%
GAATTCATTTGGTCTGATGG+GGG - chr3_4:69490867-69490886 Msa0517770:intron 40.0%
! TGTCCATGAGTTGACACAAT+GGG + chr3_4:69491075-69491094 None:intergenic 40.0%
! ATGTCCATGAGTTGACACAA+TGG + chr3_4:69491076-69491095 None:intergenic 40.0%
CATGGACATTATTAGACCTG+TGG - chr3_4:69491087-69491106 Msa0517770:CDS 40.0%
TGAGTAACTTCTACTCCTTC+AGG + chr3_4:69491337-69491356 None:intergenic 40.0%
! GAGACACAATCTTTTGAGCA+GGG + chr3_4:69491399-69491418 None:intergenic 40.0%
! AGAGACACAATCTTTTGAGC+AGG + chr3_4:69491400-69491419 None:intergenic 40.0%
AGCAGGATTATGTGTCTTGT+AGG - chr3_4:69491562-69491581 Msa0517770:intron 40.0%
!!! GTAGGTTTTCATTCGGTGTT+TGG + chr3_4:69491727-69491746 None:intergenic 40.0%
AATCCCCAATCCAAACCTAT+TGG - chr3_4:69492116-69492135 Msa0517770:CDS 40.0%
CATCCAATAGGTTTGGATTG+GGG + chr3_4:69492122-69492141 None:intergenic 40.0%
GCACAATGTCTTCATCCAAT+AGG + chr3_4:69492134-69492153 None:intergenic 40.0%
CTTCACACATCTCCAGTTTG+AGG + chr3_4:69489699-69489718 None:intergenic 45.0%
CTCAAACTGGAGATGTGTGA+AGG - chr3_4:69489697-69489716 Msa0517770:three_prime_UTR 45.0%
CTGGAGATGTGTGAAGGATT+TGG - chr3_4:69489703-69489722 Msa0517770:three_prime_UTR 45.0%
GGGCAATCACACGTAGATTA+CGG - chr3_4:69489870-69489889 Msa0517770:three_prime_UTR 45.0%
GGCAATCACACGTAGATTAC+GGG - chr3_4:69489871-69489890 Msa0517770:three_prime_UTR 45.0%
ACACGTAGATTACGGGAAGA+TGG - chr3_4:69489878-69489897 Msa0517770:three_prime_UTR 45.0%
TCTGTGATTGACAACTCTGC+TGG - chr3_4:69490189-69490208 Msa0517770:intron 45.0%
CGTGCAAGTCACCATATGAA+TGG - chr3_4:69490266-69490285 Msa0517770:intron 45.0%
CTTAGGCGTCTAGCATCATA+TGG - chr3_4:69490480-69490499 Msa0517770:CDS 45.0%
TCACTGTTGTGCCATCTACA+TGG - chr3_4:69490511-69490530 Msa0517770:CDS 45.0%
! GTACTTTTCAGCAATGGTCC+TGG - chr3_4:69490570-69490589 Msa0517770:intron 45.0%
TGCATGGGACATCAATTGCT+AGG - chr3_4:69490666-69490685 Msa0517770:intron 45.0%
GAACAGGATGATTTCCTCCA+TGG + chr3_4:69490734-69490753 None:intergenic 45.0%
TTCATAGAAGGGTCAGAACC+TGG - chr3_4:69490935-69490954 Msa0517770:intron 45.0%
GGCACTATCATAGTGAGTTC+TGG + chr3_4:69490992-69491011 None:intergenic 45.0%
! TCCATGAGTTGACACAATGG+GGG + chr3_4:69491073-69491092 None:intergenic 45.0%
! GTCCATGAGTTGACACAATG+GGG + chr3_4:69491074-69491093 None:intergenic 45.0%
CTCAATGACAGTAGTTGTGC+CGG - chr3_4:69491364-69491383 Msa0517770:CDS 45.0%
TTGTCAGAATCATGAGGTCC+AGG + chr3_4:69491445-69491464 None:intergenic 45.0%
GGACCTCATGATTCTGACAA+CGG - chr3_4:69491445-69491464 Msa0517770:intron 45.0%
TCACCGTTGTCAGAATCATG+AGG + chr3_4:69491451-69491470 None:intergenic 45.0%
! ACACTGATTGCAGTTGGTGT+AGG + chr3_4:69491745-69491764 None:intergenic 45.0%
CTTTATCCTTCTAGCCTGGT+GGG + chr3_4:69491793-69491812 None:intergenic 45.0%
ACTTTATCCTTCTAGCCTGG+TGG + chr3_4:69491794-69491813 None:intergenic 45.0%
GCTAGCCCTTGTACAGAAAA+TGG + chr3_4:69491916-69491935 None:intergenic 45.0%
CTCGTAGCCTTCATTGAAGA+AGG - chr3_4:69491952-69491971 Msa0517770:CDS 45.0%
! TGGGAGTTTAGCTCCAGTGA+AGG - chr3_4:69490310-69490329 Msa0517770:intron 50.0%
! GGGAGTTTAGCTCCAGTGAA+GGG - chr3_4:69490311-69490330 Msa0517770:intron 50.0%
AGTATCTCTAGGCCCTTCAC+TGG + chr3_4:69490326-69490345 None:intergenic 50.0%
ACGGCATAAGGAACAGCAGA+AGG + chr3_4:69490600-69490619 None:intergenic 50.0%
CAATTGCTAGGCCAGGCTTT+AGG - chr3_4:69490678-69490697 Msa0517770:intron 50.0%
AGGTTGTACCTCCTAAAGCC+TGG + chr3_4:69490692-69490711 None:intergenic 50.0%
CCAGTTCGAAGGTTACGAAC+AGG + chr3_4:69490750-69490769 None:intergenic 50.0%
CCTGTTCGTAACCTTCGAAC+TGG - chr3_4:69490747-69490766 Msa0517770:intron 50.0%
TTTCAACACGGCCAGTTCGA+AGG + chr3_4:69490761-69490780 None:intergenic 50.0%
TTCATTTGGTCTGATGGGGG+AGG - chr3_4:69490870-69490889 Msa0517770:intron 50.0%
TCATTTGGTCTGATGGGGGA+GGG - chr3_4:69490871-69490890 Msa0517770:intron 50.0%
TCTTCTCAAGACAAGGGACC+AGG + chr3_4:69490956-69490975 None:intergenic 50.0%
! CTCACTATGATAGTGCCTCG+TGG - chr3_4:69490995-69491014 Msa0517770:intron 50.0%
! TGGGGGCATAACAGAACTGA+GGG + chr3_4:69491056-69491075 None:intergenic 50.0%
! ATGGGGGCATAACAGAACTG+AGG + chr3_4:69491057-69491076 None:intergenic 50.0%
CAGGGTCAACTGCATAGTTG+TGG + chr3_4:69491306-69491325 None:intergenic 50.0%
TTCTACTCCTTCAGGCAGTG+TGG + chr3_4:69491329-69491348 None:intergenic 50.0%
TTGAGCAGGGAAAGCAAGAC+CGG + chr3_4:69491386-69491405 None:intergenic 50.0%
! CGGGAAGATGGCAGCCTTAT+TGG - chr3_4:69489890-69489909 Msa0517770:three_prime_UTR 55.0%
TCGGATGCTGGCCATGTAGA+TGG + chr3_4:69490525-69490544 None:intergenic 55.0%
GGAACAGCAGAAGGATCTCC+AGG + chr3_4:69490591-69490610 None:intergenic 55.0%
GGGACATCAATTGCTAGGCC+AGG - chr3_4:69490671-69490690 Msa0517770:intron 55.0%
!! TTGCTAGGCCAGGCTTTAGG+AGG - chr3_4:69490681-69490700 Msa0517770:intron 55.0%
GCCCCCATTGTGTCAACTCA+TGG - chr3_4:69491069-69491088 Msa0517770:CDS 55.0%
! TACCACCCTGAAGCATCTCC+TGG - chr3_4:69491424-69491443 Msa0517770:intron 55.0%
GAGGTCCAGGAGATGCTTCA+GGG + chr3_4:69491432-69491451 None:intergenic 55.0%
TGAGGTCCAGGAGATGCTTC+AGG + chr3_4:69491433-69491452 None:intergenic 55.0%
AAGCTAGCTGCTATCCCACC+AGG - chr3_4:69491776-69491795 Msa0517770:intron 55.0%
TGCTATCCCACCAGGCTAGA+AGG - chr3_4:69491784-69491803 Msa0517770:intron 55.0%
! TACAAGGGCTAGCAGCCGAT+TGG - chr3_4:69491923-69491942 Msa0517770:intron 55.0%
GGCTACGAGCCTATGCCAAT+CGG + chr3_4:69491941-69491960 None:intergenic 55.0%
ACTGCGGCACTATAGCGCAG+CGG - chr3_4:69490048-69490067 Msa0517770:three_prime_UTR 60.0%
CTCCTTCAGGCAGTGTGGCA+GGG + chr3_4:69491324-69491343 None:intergenic 60.0%
ACTCCTTCAGGCAGTGTGGC+AGG + chr3_4:69491325-69491344 None:intergenic 60.0%
GTCCAGGAGATGCTTCAGGG+TGG + chr3_4:69491429-69491448 None:intergenic 60.0%
GGCTAGCAGCCGATTGGCAT+AGG - chr3_4:69491929-69491948 Msa0517770:CDS 60.0%
GACCCTGCCACACTGCCTGA+AGG - chr3_4:69491319-69491338 Msa0517770:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 69489665 69492173 69489665 ID=Msa0517770;Name=Msa0517770
chr3_4 mRNA 69489665 69492173 69489665 ID=Msa0517770-mRNA-1;Parent=Msa0517770;Name=Msa0517770-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.6|0.5|1|0.8|0.83|6|407|279
chr3_4 exon 69489665 69490154 69489665 ID=Msa0517770-mRNA-1:exon:18544;Parent=Msa0517770-mRNA-1
chr3_4 exon 69490372 69490537 69490372 ID=Msa0517770-mRNA-1:exon:18543;Parent=Msa0517770-mRNA-1
chr3_4 exon 69491047 69491394 69491047 ID=Msa0517770-mRNA-1:exon:18542;Parent=Msa0517770-mRNA-1
chr3_4 exon 69491487 69491575 69491487 ID=Msa0517770-mRNA-1:exon:18541;Parent=Msa0517770-mRNA-1
chr3_4 exon 69491927 69491983 69491927 ID=Msa0517770-mRNA-1:exon:18540;Parent=Msa0517770-mRNA-1
chr3_4 exon 69492077 69492173 69492077 ID=Msa0517770-mRNA-1:exon:18539;Parent=Msa0517770-mRNA-1
chr3_4 CDS 69492077 69492173 69492077 ID=Msa0517770-mRNA-1:cds;Parent=Msa0517770-mRNA-1
chr3_4 CDS 69491927 69491983 69491927 ID=Msa0517770-mRNA-1:cds;Parent=Msa0517770-mRNA-1
chr3_4 CDS 69491487 69491575 69491487 ID=Msa0517770-mRNA-1:cds;Parent=Msa0517770-mRNA-1
chr3_4 CDS 69491047 69491394 69491047 ID=Msa0517770-mRNA-1:cds;Parent=Msa0517770-mRNA-1
chr3_4 CDS 69490372 69490537 69490372 ID=Msa0517770-mRNA-1:cds;Parent=Msa0517770-mRNA-1
chr3_4 CDS 69490072 69490154 69490072 ID=Msa0517770-mRNA-1:cds;Parent=Msa0517770-mRNA-1
chr3_4 three_prime_UTR 69489665 69490071 69489665 ID=Msa0517770-mRNA-1:three_prime_utr;Parent=Msa0517770-mRNA-1
Gene Sequence

>Msa0517770

CTGTCTCTTTGCGATGGATATTTATTCAGTACCTCAAACTGGAGATGTGTGAAGGATTTGGGCGATTACTTAACTGAAAGGAATGTCATGGGAATTTATGATGTTGATACAAGGGCAATCACACGTAGATTACGGGAAGATGGCAGCCTTATTGTATCGTGCAAGTCACCATATGAATGGATTGATAGAACAAAGGAAGAATGGGAGTTTAGCTCCAGTGAAGGGCCTAGAGATACTTTCCATGTAGTTGCATACGATTTTGGAATCAAGCATAATATACTTAGGCGTCTAGCATCATATGGCTGTAAAATCACTGTTGTGCCATCTACATGGCCAGCATCCGAAACTTTGAAGTTAAATCCAGATGGTGTACTTTTCAGCAATGGTCCTGGAGATCCTTCTGCTGTTCCTTATGCCGTTGAAACAGTAAAGAATATCATTGGAAAGGTGCCTGTTTTTGGAATTTGCATGGGACATCAATTGCTAGGCCAGGCTTTAGGAGGTACAACCTACAAGATGAAATTTGGTCACCATGGAGGAAATCATCCTGTTCGTAACCTTCGAACTGGCCGTGTTGAAATTAGTTCTCAGAACCACAACTATGCAGTTGACCCTGCCACACTGCCTGAAGGAGTAGAAGTTACTCATATCAATCTCAATGACAGTAGTTGTGCCGGTCTTGCTTTCCCTGCTCAAAAGATTGTGTCTCTTCAATACCACCCTGAAGCATCTCCTGGACCTCATGATTCTGACAACGCTTTTCGGGAGTTTATAGAGCTAATGAAGCATGAGAAAAACCAAACACCGAATGAAAACCTACACCAACTGCAATCAGTGTAA
Protein sequence

>Msa0517770

LSLCDGYLFSTSNWRCVKDLGDYLTERNVMGIYDVDTRAITRRLREDGSLIVSCKSPYEWIDRTKEEWEFSSSEGPRDTFHVVAYDFGIKHNILRRLASYGCKITVVPSTWPASETLKLNPDGVLFSNGPGDPSAVPYAVETVKNIIGKVPVFGICMGHQLLGQALGGTTYKMKFGHHGGNHPVRNLRTGRVEISSQNHNYAVDPATLPEGVEVTHINLNDSSCAGLAFPAQKIVSLQYHPEASPGPHDSDNAFREFIELMKHEKNQTPNENLHQLQSV*