AlfalfaGEDB | Msa0517840

M. sativa cultivar zhongmu-4 / Msa0517840

KEGG: K01956
GO: GO:0000050 GO:0003674 GO:0003824 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494
PFAM: PF00988.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0517840	XP_024635676.1	91.435	432	6	1	1	401	1	432	0.0	814

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0517840	sp\|Q9LVW7\|CARA_ARATH	67.599	429	99	3	1	390	3	430	0.0	601

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0517840	A0A396IZL0	91.435	432	6	1	1	401	1	432	0.0	814

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0517840	-0.806195	9.256263e-50	-8.615850e-47
Msa0030380	Msa0517840	0.809615	1.733799e-50	-8.615850e-47
Msa0069260	Msa0517840	0.819777	9.719895e-53	-8.615850e-47
Msa0069280	Msa0517840	0.810637	1.044088e-50	-8.615850e-47
Msa0069310	Msa0517840	0.818017	2.440904e-52	-8.615850e-47
Msa0145950	Msa0517840	0.808887	2.482683e-50	-8.615850e-47
Msa0148120	Msa0517840	0.811378	7.215263e-51	-8.615850e-47
Msa0168640	Msa0517840	-0.813167	2.935355e-51	-8.615850e-47
Msa0172350	Msa0517840	0.826400	2.769566e-54	-8.615850e-47
Msa0173740	Msa0517840	0.810827	9.499268e-51	-8.615850e-47
Msa0173820	Msa0517840	0.810864	9.325315e-51	-8.615850e-47
Msa0178990	Msa0517840	0.809102	2.234029e-50	-8.615850e-47
Msa0188890	Msa0517840	0.822026	2.951799e-53	-8.615850e-47
Msa0231380	Msa0517840	0.812944	3.285100e-51	-8.615850e-47
Msa0235120	Msa0517840	0.805231	1.475142e-49	-8.615850e-47
Msa0236270	Msa0517840	0.804525	2.072072e-49	-8.615850e-47
Msa0376040	Msa0517840	0.804979	1.665185e-49	-8.615850e-47
Msa0377530	Msa0517840	0.826545	2.557945e-54	-8.615850e-47
Msa0380040	Msa0517840	0.842975	1.862163e-58	-8.615850e-47
Msa0392940	Msa0517840	0.812404	4.312890e-51	-8.615850e-47
Msa0412440	Msa0517840	0.806539	7.831348e-50	-8.615850e-47
Msa0426810	Msa0517840	0.820821	5.601749e-53	-8.615850e-47
Msa0427220	Msa0517840	0.820595	6.313178e-53	-8.615850e-47
Msa0429290	Msa0517840	0.863888	1.804579e-64	-8.615850e-47
Msa0429950	Msa0517840	0.812104	5.014056e-51	-8.615850e-47
Msa0449820	Msa0517840	0.808851	2.527750e-50	-8.615850e-47
Msa0456910	Msa0517840	0.806910	6.539237e-50	-8.615850e-47
Msa0469790	Msa0517840	0.805114	1.560335e-49	-8.615850e-47
Msa0473240	Msa0517840	0.801094	1.058782e-48	-8.615850e-47
Msa0473720	Msa0517840	0.901531	2.439711e-78	-8.615850e-47
Msa0475580	Msa0517840	0.855667	5.407732e-62	-8.615850e-47
Msa0242680	Msa0517840	0.823299	1.492303e-53	-8.615850e-47
Msa0244270	Msa0517840	0.802179	6.344374e-49	-8.615850e-47
Msa0267760	Msa0517840	0.820021	8.546564e-53	-8.615850e-47
Msa0270660	Msa0517840	0.813308	2.733330e-51	-8.615850e-47
Msa0302650	Msa0517840	0.805294	1.430927e-49	-8.615850e-47
Msa0304290	Msa0517840	0.800042	1.734766e-48	-8.615850e-47
Msa0313740	Msa0517840	0.801649	8.150356e-49	-8.615850e-47
Msa0358170	Msa0517840	0.803923	2.765250e-49	-8.615850e-47
Msa0493920	Msa0517840	0.804639	1.961313e-49	-8.615850e-47
Msa0516040	Msa0517840	0.802201	6.277193e-49	-8.615850e-47
Msa0517490	Msa0517840	0.830914	2.244842e-55	-8.615850e-47
Msa0517770	Msa0517840	0.951967	6.651440e-110	-8.615850e-47
Msa0517840	Msa0519220	0.854662	1.059869e-61	-8.615850e-47
Msa0517840	Msa0526710	0.820207	7.746617e-53	-8.615850e-47
Msa0517840	Msa0530850	0.825773	3.905223e-54	-8.615850e-47
Msa0517840	Msa0535030	0.811149	8.089182e-51	-8.615850e-47
Msa0517840	Msa0535060	0.810296	1.236948e-50	-8.615850e-47
Msa0517840	Msa0553150	0.826465	2.673208e-54	-8.615850e-47
Msa0517840	Msa0587690	0.807307	5.388794e-50	-8.615850e-47
Msa0517840	Msa0607510	0.838608	2.599120e-57	-8.615850e-47
Msa0517840	Msa0614990	0.806798	6.906844e-50	-8.615850e-47
Msa0517840	Msa0669850	0.809156	2.174455e-50	-8.615850e-47
Msa0517840	Msa0690250	0.814872	1.234770e-51	-8.615850e-47
Msa0517840	Msa0690550	0.839002	2.055779e-57	-8.615850e-47
Msa0517840	Msa0711490	0.806464	8.121490e-50	-8.615850e-47
Msa0517840	Msa0743260	0.800575	1.351668e-48	-8.615850e-47
Msa0517840	Msa0790610	0.815451	9.185470e-52	-8.615850e-47
Msa0517840	Msa0824730	0.802056	6.722901e-49	-8.615850e-47
Msa0517840	Msa0874500	0.803119	4.059445e-49	-8.615850e-47
Msa0517840	Msa0922540	0.817993	2.471195e-52	-8.615850e-47
Msa0517840	Msa0984210	0.870515	1.376505e-66	-8.615850e-47
Msa0517840	Msa1001580	0.800209	1.604849e-48	-8.615850e-47
Msa0517840	Msa1077670	0.837574	4.796550e-57	-8.615850e-47
Msa0517840	Msa1109570	0.807177	5.740848e-50	-8.615850e-47
Msa0517840	Msa1260130	0.807743	4.355238e-50	-8.615850e-47
Msa0517840	Msa1306580	0.810564	1.082738e-50	-8.615850e-47
Msa0517840	Msa1343420	0.800969	1.123083e-48	-8.615850e-47
Msa0517840	Msa1362510	0.812456	4.201754e-51	-8.615850e-47
Msa0517840	Msa1370620	0.805081	1.585351e-49	-8.615850e-47
Msa0517840	Msa1390310	0.820087	8.256608e-53	-8.615850e-47
Msa0517840	Msa1406040	0.812820	3.496795e-51	-8.615850e-47
Msa0517840	Msa1427830	0.806139	9.510870e-50	-8.615850e-47
Msa0517840	Msa1452470	0.827480	1.528922e-54	-8.615850e-47
Msa0517840	Msa1453620	0.801020	1.096578e-48	-8.615850e-47
Msa0517840	Msa1454110	0.814785	1.291006e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0517840	MtrunA17_Chr3g0129631	91.435	432	6	1	1	401	1	432	0.0	814
Msa0517840	MtrunA17_Chr4g0057811	88.194	432	19	2	1	401	1	431	0.0	783

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0517840	AT3G27740.1	67.599	429	99	3	1	390	3	430	0.0	601
Msa0517840	AT3G27740.2	62.563	398	99	7	1	390	3	358	1.42e-173	489
Msa0517840	AT1G25220.1	28.571	189	122	5	213	389	86	273	8.79e-13	68.6
Msa0517840	AT1G25220.2	28.079	203	131	6	201	389	85	286	2.60e-12	67.4
Msa0517840	AT1G24909.1	28.042	189	123	5	213	389	32	219	4.79e-12	65.5
Msa0517840	AT1G25083.1	28.042	189	123	5	213	389	32	219	4.79e-12	65.5
Msa0517840	AT1G25155.1	28.042	189	123	5	213	389	32	219	4.79e-12	65.5
Msa0517840	AT5G57890.1	27.513	189	124	5	213	389	83	270	5.15e-12	66.2
Msa0517840	AT1G24807.1	28.342	187	121	5	215	389	47	232	1.70e-11	64.3

Find 144 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGAATACTGGCATCAATTT+TGG	0.168281	3_4:-69563648	Msa0517840:intron
CGGGAAGATGGCAGCCTTAT+TGG	0.173503	3_4:-69563167	Msa0517840:intron
TCAGCACAGTGCTTCCTTTC+CGG	0.240008	3_4:-69563522	Msa0517840:CDS
TAATTAGAAGTTTGAGTATC+AGG	0.243391	3_4:-69563494	Msa0517840:intron
CTTTCTCTCAATGACCTCTT+TGG	0.245128	3_4:-69564182	Msa0517840:CDS
TTCTTAGTAGTTTCTATCTA+TGG	0.264325	3_4:-69561217	Msa0517840:three_prime_UTR
GCTAAATCATTTGGTGCTTC+AGG	0.275622	3_4:-69563886	Msa0517840:CDS
TTCTCTGTCAGATGTTCTTC+TGG	0.292462	3_4:-69564119	Msa0517840:intron
GCTAGCCCTTGTACAGAAAA+TGG	0.293625	3_4:+69561144	None:intergenic
ACAACCTACAAGATGAAATT+TGG	0.299468	3_4:-69562352	Msa0517840:CDS
AGAAGAAAGAAACATATTAA+TGG	0.305952	3_4:-69564228	Msa0517840:exon
TGTTAGAAGATGGTTCAATT+TGG	0.310513	3_4:-69563912	Msa0517840:CDS
CTATGGTTTGTAGATGAATA+AGG	0.315269	3_4:-69561200	Msa0517840:three_prime_UTR
CACAACTTTCTTGCAATTGT+GGG	0.322795	3_4:-69560758	Msa0517840:three_prime_UTR
TGGATTTAACTTCAAAGTTT+CGG	0.329533	3_4:+69562516	None:intergenic
CTGGAGATGTGTGAAGGATT+TGG	0.330063	3_4:-69563354	Msa0517840:CDS
TAGGTAGTTGCATACGATTT+TGG	0.336986	3_4:-69562616	Msa0517840:CDS
ATTTGGAAAGCTAAATCATT+TGG	0.341512	3_4:-69563895	Msa0517840:CDS
CAATTGCTAGGCCAGGCTTT+AGG	0.351638	3_4:-69562379	Msa0517840:CDS
TGAGGGCCAGGAGATGCTTC+AGG	0.357210	3_4:+69561627	None:intergenic
AACTTCAAAGTTTCGGATGC+TGG	0.363527	3_4:+69562523	None:intergenic
GTGACCAAATTTCATCTTGT+AGG	0.368330	3_4:+69562348	None:intergenic
AATCCACATATTGGGAATAC+TGG	0.375932	3_4:-69563660	Msa0517840:CDS
TCATCCAATAGGTTTGGATT+GGG	0.377058	3_4:+69560937	None:intergenic
GGTTGCTGCTAACACTGGTT+TGG	0.380442	3_4:-69564299	Msa0517840:five_prime_UTR
GCACAATGTCTTCATCCAAT+AGG	0.380576	3_4:+69560926	None:intergenic
AATCCCCAATCCAAACCTAT+TGG	0.381071	3_4:-69560941	Msa0517840:three_prime_UTR
GTTCTATCAATCCATTCATA+TGG	0.384320	3_4:+69562780	None:intergenic
ACTCCTTCAGGCAGTGTGGC+AGG	0.385852	3_4:+69561735	None:intergenic
TGCTATCCCACCAGGCTAGA+AGG	0.386176	3_4:-69561273	Msa0517840:three_prime_UTR
TGTTATGTAGTCATCAAGTT+TGG	0.389136	3_4:+69560832	None:intergenic
GGGCAATCACACGTAGATTA+CGG	0.396622	3_4:-69563187	Msa0517840:CDS
TAACACATGCAATTTGCAAT+TGG	0.396704	3_4:-69560814	Msa0517840:three_prime_UTR
CTGATGACAAATCCACATAT+TGG	0.396769	3_4:-69563669	Msa0517840:CDS
ATGTCTTCATCCAATAGGTT+TGG	0.401382	3_4:+69560931	None:intergenic
TGAAAACAACTTCACCAACT+TGG	0.401704	3_4:+69563860	None:intergenic
TCGGATGCTGGCCATGTAGA+TGG	0.404003	3_4:+69562535	None:intergenic
TGAGTAACTTCTACTCCTTC+AGG	0.404731	3_4:+69561723	None:intergenic
TTTACTGTTTCAACGGCATA+AGG	0.405190	3_4:+69562448	None:intergenic
ATTAATGGCAACAAAAGCTT+TGG	0.405497	3_4:-69564213	Msa0517840:exon
TGCATGAATGTATAGGTAAT+GGG	0.406728	3_4:-69563969	Msa0517840:intron
TCACAACTTTCTTGCAATTG+TGG	0.413831	3_4:-69560759	Msa0517840:three_prime_UTR
CAAACTTCTAATTACTAAAC+CGG	0.415332	3_4:+69563503	None:intergenic
GGCAATCACACGTAGATTAC+GGG	0.418709	3_4:-69563186	Msa0517840:CDS
TGGAGATGTGTGAAGGATTT+GGG	0.420135	3_4:-69563353	Msa0517840:CDS
TTCATCCAATAGGTTTGGAT+TGG	0.422242	3_4:+69560936	None:intergenic
TTGATAGAACAAAGGAAGAA+TGG	0.426377	3_4:-69562767	Msa0517840:CDS
TGATGACAAATCCACATATT+GGG	0.432957	3_4:-69563668	Msa0517840:CDS
AGCGATTAGCTAATAAAGTA+AGG	0.433096	3_4:+69560967	None:intergenic
ATTTATTCAGTACCTCAAAC+TGG	0.433137	3_4:-69563373	Msa0517840:intron
CAGTGCTCAAAACACCAATA+AGG	0.434979	3_4:+69563153	None:intergenic
TTAACTGATCCTAGTTATGC+TGG	0.440990	3_4:-69563702	Msa0517840:CDS
CTTCACACATCTCCAGTTTG+AGG	0.444880	3_4:+69563361	None:intergenic
CCGTGTTGAAATTAGTTCTC+AGG	0.452192	3_4:-69562287	Msa0517840:intron
AATACTTTATCCTTCTAGCC+TGG	0.459597	3_4:+69561263	None:intergenic
CTTAGGCGTCTAGCATCATA+TGG	0.460279	3_4:-69562577	Msa0517840:CDS
TGTGACCACTTGCAGCTAAA+GGG	0.464808	3_4:+69560777	None:intergenic
CAAAGAGGTCATTGAGAGAA+AGG	0.466089	3_4:+69564183	None:intergenic
GAAACAGTAAAGAATATCAT+TGG	0.466180	3_4:-69562436	Msa0517840:CDS
GATTTGTCATCAGCACAAAC+TGG	0.471155	3_4:+69563679	None:intergenic
ACATTATCCTTCTTCAATGA+AGG	0.472185	3_4:+69561098	None:intergenic
GCTAGACTTGTGTTAGAAGA+TGG	0.472551	3_4:-69563922	Msa0517840:CDS
TGATAGAACAAAGGAAGAAT+GGG	0.475190	3_4:-69562766	Msa0517840:CDS
TTGCTGAAAAGTACACCATC+TGG	0.479305	3_4:+69562496	None:intergenic
GAAAACAACTTCACCAACTT+GGG	0.481798	3_4:+69563861	None:intergenic
CGTGCAAGTCACCATATGAA+TGG	0.483758	3_4:-69562791	Msa0517840:CDS
GGAACAGCAGAAGGATCTCC+AGG	0.484183	3_4:+69562469	None:intergenic
CAATGACCTCTTTGGTTCCA+AGG	0.486297	3_4:-69564174	Msa0517840:CDS
ATGCCAGTATTCCCAATATG+TGG	0.486638	3_4:+69563657	None:intergenic
TTGTGACCACTTGCAGCTAA+AGG	0.489830	3_4:+69560776	None:intergenic
CCGTGCAGCTTGTAGTTGAA+AGG	0.490653	3_4:-69560723	None:intergenic
AAAGTATGTGATAAAAGCTC+AGG	0.494537	3_4:-69561240	Msa0517840:three_prime_UTR
TAGATGAATAAGGACTTCCT+TGG	0.495299	3_4:-69561190	Msa0517840:three_prime_UTR
GATATTCTTTACTGTTTCAA+CGG	0.495805	3_4:+69562441	None:intergenic
TTGAGCAGGGAAAGCAAGAC+CGG	0.499137	3_4:+69561674	None:intergenic
CTCCTTCAGGCAGTGTGGCA+GGG	0.505914	3_4:+69561736	None:intergenic
GAATCAAGCATAATATACTT+AGG	0.506239	3_4:-69562594	Msa0517840:CDS
TACCACCCTGAAGCATCTCC+TGG	0.507000	3_4:-69561633	Msa0517840:CDS
AGAGGTGTGTGGCGGCGCCT+TGG	0.508323	3_4:+69564157	None:intergenic
ACAACTTTCTTGCAATTGTG+GGG	0.513003	3_4:-69560757	Msa0517840:three_prime_UTR
TTGTCAGAATCATGAGGGCC+AGG	0.515466	3_4:+69561615	None:intergenic
CTCGTAGCCTTCATTGAAGA+AGG	0.515567	3_4:-69561105	Msa0517840:three_prime_UTR
CCAGTTCGAAGGTTACGAAC+AGG	0.516140	3_4:+69562310	None:intergenic
CTCAATGACAGTAGTTGTGC+CGG	0.517106	3_4:-69561693	Msa0517840:CDS
ACAAACTGGCCAGCATAACT+AGG	0.518582	3_4:+69563693	None:intergenic
CTTAACTGAAAGGAATGTCA+TGG	0.522571	3_4:-69563324	Msa0517840:CDS
GGGACATCAATTGCTAGGCC+AGG	0.526918	3_4:-69562386	Msa0517840:CDS
AGTATCTCTAGGCCCTTCAC+TGG	0.527684	3_4:+69562734	None:intergenic
CTTTATCCTTCTAGCCTGGT+GGG	0.529670	3_4:+69561267	None:intergenic
CAACTTTCTTGCAATTGTGG+GGG	0.530199	3_4:-69560756	Msa0517840:three_prime_UTR
GAGGGCCAGGAGATGCTTCA+GGG	0.531645	3_4:+69561628	None:intergenic
CCTGTTCGTAACCTTCGAAC+TGG	0.534549	3_4:-69562310	Msa0517840:CDS
CATCCAATAGGTTTGGATTG+GGG	0.536858	3_4:+69560938	None:intergenic
TGGGCGATTACTTAACTGAA+AGG	0.537172	3_4:-69563334	Msa0517840:CDS
CTTACATGGAAAGTATCTCT+AGG	0.537477	3_4:+69562723	None:intergenic
TAGGTAATGGGGAAAGGCCA+TGG	0.546071	3_4:-69563957	Msa0517840:CDS
GGTGCTTCAGGAACCCAAGT+TGG	0.546817	3_4:-69563874	Msa0517840:CDS
AGGTTGTACCTCCTAAAGCC+TGG	0.548433	3_4:+69562368	None:intergenic
CAACAGATGATGTTGATACA+AGG	0.549921	3_4:-69563208	Msa0517840:intron
ACACTGATTGCAGTTGGTGT+AGG	0.551642	3_4:+69561315	None:intergenic
ACACGTAGATTACGGGAAGA+TGG	0.551699	3_4:-69563179	Msa0517840:CDS
AAAGAGGTCATTGAGAGAAA+GGG	0.551745	3_4:+69564184	None:intergenic
GGCTAGCAGCCGATTGGCAT+AGG	0.554150	3_4:-69561128	Msa0517840:three_prime_UTR
TACAAGGGCTAGCAGCCGAT+TGG	0.556106	3_4:-69561134	Msa0517840:three_prime_UTR
TGCATCCCTTTAGCTGCAAG+TGG	0.560181	3_4:-69560782	Msa0517840:three_prime_UTR
AATGTATAGGTAATGGGGAA+AGG	0.560392	3_4:-69563963	Msa0517840:intron
TCACTGTTGTGCCATCTACA+TGG	0.561762	3_4:-69562546	Msa0517840:CDS
GAACAGGATGATTTCCTCCA+TGG	0.563497	3_4:+69562326	None:intergenic
ACATAGGTTGCTGCTAACAC+TGG	0.565602	3_4:-69564304	Msa0517840:five_prime_UTR
AAGCTAGCTGCTATCCCACC+AGG	0.568493	3_4:-69561281	Msa0517840:three_prime_UTR
TTTCAACACGGCCAGTTCGA+AGG	0.571733	3_4:+69562299	None:intergenic
GCATGAATGTATAGGTAATG+GGG	0.572178	3_4:-69563968	Msa0517840:intron
CAGGGTCAACTGCATAGTTG+TGG	0.573224	3_4:+69561754	None:intergenic
TGCATGGGACATCAATTGCT+AGG	0.573890	3_4:-69562391	Msa0517840:CDS
AGTAAAGAATATCATTGGAA+AGG	0.576011	3_4:-69562431	Msa0517840:CDS
GGCTACGAGCCTATGCCAAT+CGG	0.576216	3_4:+69561119	None:intergenic
TTGCTAGGCCAGGCTTTAGG+AGG	0.580876	3_4:-69562376	Msa0517840:CDS
GCGATTAGCTAATAAAGTAA+GGG	0.585873	3_4:+69560968	None:intergenic
ATTATTACACTGATTGCAGT+TGG	0.586719	3_4:+69561309	None:intergenic
GACCCTGCCACACTGCCTGA+AGG	0.593729	3_4:-69561738	Msa0517840:CDS
GAAAATCTTGATCAACTCCA+AGG	0.593981	3_4:+69561173	None:intergenic
TTAACTGAAAGGAATGTCAT+GGG	0.597258	3_4:-69563323	Msa0517840:CDS
GTCATTGAGAGAAAGGGTGA+AGG	0.598395	3_4:+69564190	None:intergenic
TGGGAGTTTAGCTCCAGTGA+AGG	0.599112	3_4:-69562747	Msa0517840:CDS
ACTTTATCCTTCTAGCCTGG+TGG	0.599459	3_4:+69561266	None:intergenic
GGCCCTCATGATTCTGACAA+CGG	0.603798	3_4:-69561612	Msa0517840:intron
ACGGCATAAGGAACAGCAGA+AGG	0.606024	3_4:+69562460	None:intergenic
CTCAAACTGGAGATGTGTGA+AGG	0.613677	3_4:-69563360	Msa0517840:CDS
AAGATGAAATTTGGTCACCA+TGG	0.620210	3_4:-69562343	Msa0517840:CDS
ACTTTGAAGTTAAATCCAGA+TGG	0.620988	3_4:-69562511	Msa0517840:CDS
ATGAAATTTGGTCACCATGG+AGG	0.624582	3_4:-69562340	Msa0517840:CDS
TTCTAATTACTAAACCGGAA+AGG	0.626092	3_4:+69563508	None:intergenic
TGAATGGATTGATAGAACAA+AGG	0.627214	3_4:-69562775	Msa0517840:CDS
CCTAGGTGAAGAGAAAACAT+AGG	0.629266	3_4:-69564320	Msa0517840:five_prime_UTR
GGGAGTTTAGCTCCAGTGAA+GGG	0.629622	3_4:-69562746	Msa0517840:CDS
AACAGATGATGTTGATACAA+GGG	0.640054	3_4:-69563207	Msa0517840:intron
GCGGCGCCTTGGAACCAAAG+AGG	0.643056	3_4:+69564168	None:intergenic
TCACCGTTGTCAGAATCATG+AGG	0.644668	3_4:+69561609	None:intergenic
CACCGTTGTCAGAATCATGA+GGG	0.645011	3_4:+69561610	None:intergenic
TTCTACTCCTTCAGGCAGTG+TGG	0.658466	3_4:+69561731	None:intergenic
CCTTTCAACTACAAGCTGCA+CGG	0.674886	3_4:+69560723	None:intergenic
TCATTGTCTGACAGATGACG+AGG	0.685177	3_4:-69563547	Msa0517840:intron
CCTGAGAACTAATTTCAACA+CGG	0.701914	3_4:+69562287	None:intergenic
GGCCAGGAGATGCTTCAGGG+TGG	0.722700	3_4:+69561631	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTCATAATTATTTGT+TGG	-	chr3_4:69560990-69561009	Msa0517840:three_prime_UTR	10.0%
!!	ATTAATTGATACATATAAAT+TGG	-	chr3_4:69563283-69563302	Msa0517840:intron	10.0%
!!	ATATCACAAGATTTATAATT+TGG	+	chr3_4:69561252-69561271	None:intergenic	15.0%
!!	AATAAATAAAAAATTGCAAC+TGG	-	chr3_4:69563030-69563049	Msa0517840:intron	15.0%
!!!	ATAGCATAAAATTTTGAATA+TGG	-	chr3_4:69563120-69563139	Msa0517840:intron	15.0%
!!	TGATAATAAATATAACTGTT+TGG	-	chr3_4:69563515-69563534	Msa0517840:CDS	15.0%
!!!	AAATTTTTTAAAGATTGTGA+AGG	+	chr3_4:69563541-69563560	None:intergenic	15.0%
!!	CAAATTGAAAATAAAAACCT+AGG	-	chr3_4:69560728-69560747	Msa0517840:three_prime_UTR	20.0%
!!	AGAAGAAAGAAACATATTAA+TGG	-	chr3_4:69560837-69560856	Msa0517840:three_prime_UTR	20.0%
!!!	AAAAAGACAACATTTTTCAT+GGG	+	chr3_4:69561023-69561042	None:intergenic	20.0%
!!!	AAAAAAGACAACATTTTTCA+TGG	+	chr3_4:69561024-69561043	None:intergenic	20.0%
!!!	AAATGTTGTCTTTTTTGATT+TGG	-	chr3_4:69561028-69561047	Msa0517840:three_prime_UTR	20.0%
!!!	GATATTCTTTTGTTGTTTTA+TGG	-	chr3_4:69561267-69561286	Msa0517840:three_prime_UTR	20.0%
!!	TTAAGTAATTACCATGATTA+CGG	+	chr3_4:69562847-69562866	None:intergenic	20.0%
!!	TAAATCCATTTGAATTCATT+TGG	-	chr3_4:69562864-69562883	Msa0517840:intron	20.0%
!!!	ATATTTTTTTGCTTCATAGA+AGG	-	chr3_4:69562931-69562950	Msa0517840:intron	20.0%
!!!	TATTTTTTTGCTTCATAGAA+GGG	-	chr3_4:69562932-69562951	Msa0517840:intron	20.0%
!!	TTTAAACAAACCACAAATAT+AGG	+	chr3_4:69563183-69563202	None:intergenic	20.0%
!!!	GTATTTTATTCTTTTTGTCA+TGG	-	chr3_4:69563249-69563268	Msa0517840:intron	20.0%
!!	AAAAATTTATATTCAAGAGC+AGG	-	chr3_4:69563553-69563572	Msa0517840:intron	20.0%
!!!	TATTTTTACAACTTGAAATG+TGG	-	chr3_4:69564032-69564051	Msa0517840:intron	20.0%
!!!	ATTTTTACAACTTGAAATGT+GGG	-	chr3_4:69564033-69564052	Msa0517840:intron	20.0%
!!	TTCATTCAAGTAAAAAATGT+TGG	+	chr3_4:69564167-69564186	None:intergenic	20.0%
!!!	CTTGAATGAAATTTTTGTTA+TGG	-	chr3_4:69564177-69564196	Msa0517840:CDS	20.0%
!!!	TTTTTTGATTTGGTGTTGTT+GGG	-	chr3_4:69561038-69561057	Msa0517840:three_prime_UTR	25.0%
!	AAATGAATGCATGAATGTAT+AGG	-	chr3_4:69561089-69561108	Msa0517840:three_prime_UTR	25.0%
!	ATTTGGAAAGCTAAATCATT+TGG	-	chr3_4:69561170-69561189	Msa0517840:three_prime_UTR	25.0%
!!!	TTTTCAATACATCATTGACT+GGG	-	chr3_4:69561222-69561241	Msa0517840:three_prime_UTR	25.0%
!	AATGAAACAGAAACTCAATT+AGG	+	chr3_4:69561304-69561323	None:intergenic	25.0%
!	TTAAAATTTCTTGATACCTG+AGG	+	chr3_4:69561347-69561366	None:intergenic	25.0%
!	CAAACTTCTAATTACTAAAC+CGG	+	chr3_4:69561565-69561584	None:intergenic	25.0%
!	TAATTAGAAGTTTGAGTATC+AGG	-	chr3_4:69561571-69561590	Msa0517840:intron	25.0%
!	TTACCCAAAATTCAACAAAA+GGG	+	chr3_4:69562396-69562415	None:intergenic	25.0%
!	GAATCAAGCATAATATACTT+AGG	-	chr3_4:69562471-69562490	Msa0517840:CDS	25.0%
!!	TGGATTTAACTTCAAAGTTT+CGG	+	chr3_4:69562552-69562571	None:intergenic	25.0%
!	GATATTCTTTACTGTTTCAA+CGG	+	chr3_4:69562627-69562646	None:intergenic	25.0%
!	GAAACAGTAAAGAATATCAT+TGG	-	chr3_4:69562629-69562648	Msa0517840:intron	25.0%
!	AGTAAAGAATATCATTGGAA+AGG	-	chr3_4:69562634-69562653	Msa0517840:intron	25.0%
!!!	TTTTATTTATTTTTGCCACG+AGG	+	chr3_4:69563021-69563040	None:intergenic	25.0%
!!!	AAATTTTATGCTATTTCCAC+AGG	+	chr3_4:69563114-69563133	None:intergenic	25.0%
!!	CTTGATAACATGTAATGATA+TGG	+	chr3_4:69563210-69563229	None:intergenic	25.0%
!	ACGAAATTGTAAAAGCAAAT+TGG	+	chr3_4:69563611-69563630	None:intergenic	25.0%
!	TTCTTAGTAGTTTCTATCTA+TGG	-	chr3_4:69563848-69563867	Msa0517840:CDS	25.0%
!!	TTTTTACAACTTGAAATGTG+GGG	-	chr3_4:69564034-69564053	Msa0517840:intron	25.0%
!!!	TTTTTGTTATGGAAGAATGA+AGG	-	chr3_4:69564188-69564207	Msa0517840:CDS	25.0%
	ATTAATGGCAACAAAAGCTT+TGG	-	chr3_4:69560852-69560871	Msa0517840:three_prime_UTR	30.0%
!!!	CTTTTTTGATTTGGTGTTGT+TGG	-	chr3_4:69561037-69561056	Msa0517840:three_prime_UTR	30.0%
	ATGCATGAATGTATAGGTAA+TGG	-	chr3_4:69561095-69561114	Msa0517840:three_prime_UTR	30.0%
	TGCATGAATGTATAGGTAAT+GGG	-	chr3_4:69561096-69561115	Msa0517840:three_prime_UTR	30.0%
	TGTTAGAAGATGGTTCAATT+TGG	-	chr3_4:69561153-69561172	Msa0517840:three_prime_UTR	30.0%
!	GTTTTCAATACATCATTGAC+TGG	-	chr3_4:69561221-69561240	Msa0517840:three_prime_UTR	30.0%
	TGATGACAAATCCACATATT+GGG	-	chr3_4:69561397-69561416	Msa0517840:intron	30.0%
!!	TCAAGAGATGGTTTTTAAAG+TGG	+	chr3_4:69561497-69561516	None:intergenic	30.0%
	TTCTAATTACTAAACCGGAA+AGG	+	chr3_4:69561560-69561579	None:intergenic	30.0%
	AGAAAGTAAAGCTCAATCAA+CGG	+	chr3_4:69561625-69561644	None:intergenic	30.0%
	ATTTATTCAGTACCTCAAAC+TGG	-	chr3_4:69561692-69561711	Msa0517840:CDS	30.0%
	TTAACTGAAAGGAATGTCAT+GGG	-	chr3_4:69561742-69561761	Msa0517840:CDS	30.0%
	AACAGATGATGTTGATACAA+GGG	-	chr3_4:69561858-69561877	Msa0517840:intron	30.0%
	TACTTCAGATGTCTAAATCA+TGG	-	chr3_4:69561959-69561978	Msa0517840:intron	30.0%
	ACTTCAGATGTCTAAATCAT+GGG	-	chr3_4:69561960-69561979	Msa0517840:intron	30.0%
	TCTAAATCATGGGATATTGT+TGG	-	chr3_4:69561970-69561989	Msa0517840:intron	30.0%
!	TTGTTGGTAAGAATGACTAT+TGG	-	chr3_4:69561986-69562005	Msa0517840:intron	30.0%
	GTTCTATCAATCCATTCATA+TGG	+	chr3_4:69562288-69562307	None:intergenic	30.0%
!!	TGAATGGATTGATAGAACAA+AGG	-	chr3_4:69562290-69562309	Msa0517840:CDS	30.0%
	TTGATAGAACAAAGGAAGAA+TGG	-	chr3_4:69562298-69562317	Msa0517840:CDS	30.0%
	TGATAGAACAAAGGAAGAAT+GGG	-	chr3_4:69562299-69562318	Msa0517840:CDS	30.0%
	TCCATGTAAGTTTATACAGT+AGG	-	chr3_4:69562355-69562374	Msa0517840:CDS	30.0%
!	ACTCCCTTTTGTTGAATTTT+GGG	-	chr3_4:69562390-69562409	Msa0517840:CDS	30.0%
	CTTACCCAAAATTCAACAAA+AGG	+	chr3_4:69562397-69562416	None:intergenic	30.0%
!!	GTACTGATGAATGATCTTTT+AGG	-	chr3_4:69562430-69562449	Msa0517840:CDS	30.0%
!!	ACTTTGAAGTTAAATCCAGA+TGG	-	chr3_4:69562554-69562573	Msa0517840:CDS	30.0%
	ACAACCTACAAGATGAAATT+TGG	-	chr3_4:69562713-69562732	Msa0517840:intron	30.0%
!	TTGAAATTAGTTCTCAGGTA+TGG	-	chr3_4:69562783-69562802	Msa0517840:CDS	30.0%
	TCAGACCAAATGAATTCAAA+TGG	+	chr3_4:69562872-69562891	None:intergenic	30.0%
	TTTGAATTCATTTGGTCTGA+TGG	-	chr3_4:69562872-69562891	Msa0517840:intron	30.0%
	TTGAATTCATTTGGTCTGAT+GGG	-	chr3_4:69562873-69562892	Msa0517840:intron	30.0%
!	TTCTTTTCTTCTCAAGACAA+GGG	+	chr3_4:69562970-69562989	None:intergenic	30.0%
!	ATTCTTTTCTTCTCAAGACA+AGG	+	chr3_4:69562971-69562990	None:intergenic	30.0%
	TATGTGTAGTCCTATATTTG+TGG	-	chr3_4:69563170-69563189	Msa0517840:CDS	30.0%
!	ATTCTTTTTGTCATGGTCTT+TGG	-	chr3_4:69563256-69563275	Msa0517840:intron	30.0%
	GCTCTTTATCAAAAGAAGTT+TGG	-	chr3_4:69563490-69563509	Msa0517840:intron	30.0%
	GTCTTGTAGGATATAATCAT+TGG	-	chr3_4:69563583-69563602	Msa0517840:intron	30.0%
	ATCACAACAGTGGTTATTAT+TGG	+	chr3_4:69563658-69563677	None:intergenic	30.0%
	ATTATCAACAATCACAACAG+TGG	+	chr3_4:69563668-69563687	None:intergenic	30.0%
!!!	TTGTTGATAATGCAGCTTTT+CGG	-	chr3_4:69563677-69563696	Msa0517840:CDS	30.0%
!	ATTATTACACTGATTGCAGT+TGG	+	chr3_4:69563759-69563778	None:intergenic	30.0%
	AAAGTATGTGATAAAAGCTC+AGG	-	chr3_4:69563825-69563844	Msa0517840:intron	30.0%
!	CTATGGTTTGTAGATGAATA+AGG	-	chr3_4:69563865-69563884	Msa0517840:CDS	30.0%
	ACATTATCCTTCTTCAATGA+AGG	+	chr3_4:69563970-69563989	None:intergenic	30.0%
!	GCGATTAGCTAATAAAGTAA+GGG	+	chr3_4:69564100-69564119	None:intergenic	30.0%
!	AGCGATTAGCTAATAAAGTA+AGG	+	chr3_4:69564101-69564120	None:intergenic	30.0%
	TGTTATGTAGTCATCAAGTT+TGG	+	chr3_4:69564236-69564255	None:intergenic	30.0%
	TAACACATGCAATTTGCAAT+TGG	-	chr3_4:69564251-69564270	Msa0517840:five_prime_UTR	30.0%
	AAAGAGGTCATTGAGAGAAA+GGG	+	chr3_4:69560884-69560903	None:intergenic	35.0%
	GCATGAATGTATAGGTAATG+GGG	-	chr3_4:69561097-69561116	Msa0517840:three_prime_UTR	35.0%
!!	AATGTATAGGTAATGGGGAA+AGG	-	chr3_4:69561102-69561121	Msa0517840:three_prime_UTR	35.0%
!	CTAGCATCTGAATTTTTCCA+TGG	+	chr3_4:69561128-69561147	None:intergenic	35.0%
	GAAAACAACTTCACCAACTT+GGG	+	chr3_4:69561207-69561226	None:intergenic	35.0%
	TGAAAACAACTTCACCAACT+TGG	+	chr3_4:69561208-69561227	None:intergenic	35.0%
	ATTTCTTGATACCTGAGGAA+TGG	+	chr3_4:69561342-69561361	None:intergenic	35.0%
	TTAACTGATCCTAGTTATGC+TGG	-	chr3_4:69561363-69561382	Msa0517840:CDS	35.0%
	CTGATGACAAATCCACATAT+TGG	-	chr3_4:69561396-69561415	Msa0517840:intron	35.0%
	AATCCACATATTGGGAATAC+TGG	-	chr3_4:69561405-69561424	Msa0517840:intron	35.0%
!!!	TTTTAAAGTGGACTAGCACA+AGG	+	chr3_4:69561485-69561504	None:intergenic	35.0%
	TCAGACAATGAATCAAGAGA+TGG	+	chr3_4:69561509-69561528	None:intergenic	35.0%
	GCTCAATCAACGGAAAATAT+TGG	+	chr3_4:69561615-69561634	None:intergenic	35.0%
	GAAGAGACAAAGAGTAAGTA+TGG	+	chr3_4:69561653-69561672	None:intergenic	35.0%
	CTTAACTGAAAGGAATGTCA+TGG	-	chr3_4:69561741-69561760	Msa0517840:CDS	35.0%
	AGAACGTTACATGTAAACTC+TGG	-	chr3_4:69561800-69561819	Msa0517840:intron	35.0%
	CAACAGATGATGTTGATACA+AGG	-	chr3_4:69561857-69561876	Msa0517840:intron	35.0%
!	AAGTAATTCCTCGTCTGTTT+TGG	+	chr3_4:69561944-69561963	None:intergenic	35.0%
	TTGGTAAGAATGACTATTGG+AGG	-	chr3_4:69561989-69562008	Msa0517840:intron	35.0%
	AATTCAGTGTTGTCAAACTG+CGG	-	chr3_4:69562040-69562059	Msa0517840:intron	35.0%
!	GGCTTATGTTTCTTATTTGC+AGG	-	chr3_4:69562228-69562247	Msa0517840:intron	35.0%
!!	GCAGGTATTGATCTAATAAG+TGG	-	chr3_4:69562246-69562265	Msa0517840:intron	35.0%
!!	CTTACATGGAAAGTATCTCT+AGG	+	chr3_4:69562345-69562364	None:intergenic	35.0%
	GCCTACTGTATAAACTTACA+TGG	+	chr3_4:69562359-69562378	None:intergenic	35.0%
	AAGTTTATACAGTAGGCTGT+TGG	-	chr3_4:69562362-69562381	Msa0517840:CDS	35.0%
	AATTCAACAAAAGGGAGTCA+GGG	+	chr3_4:69562388-69562407	None:intergenic	35.0%
	AAATTCAACAAAAGGGAGTC+AGG	+	chr3_4:69562389-69562408	None:intergenic	35.0%
!	GACTCCCTTTTGTTGAATTT+TGG	-	chr3_4:69562389-69562408	Msa0517840:CDS	35.0%
!	TAGGTAGTTGCATACGATTT+TGG	-	chr3_4:69562449-69562468	Msa0517840:CDS	35.0%
	TTTACTGTTTCAACGGCATA+AGG	+	chr3_4:69562620-69562639	None:intergenic	35.0%
!!!	CCTGTTTTTGGAATTTGCAT+GGG	-	chr3_4:69562659-69562678	Msa0517840:intron	35.0%
	GTGACCAAATTTCATCTTGT+AGG	+	chr3_4:69562720-69562739	None:intergenic	35.0%
	AAGATGAAATTTGGTCACCA+TGG	-	chr3_4:69562722-69562741	Msa0517840:intron	35.0%
	CCTGAGAACTAATTTCAACA+CGG	+	chr3_4:69562781-69562800	None:intergenic	35.0%
	TCATCATTTCACCGTAATCA+TGG	-	chr3_4:69562833-69562852	Msa0517840:intron	35.0%
	TGAATTCATTTGGTCTGATG+GGG	-	chr3_4:69562874-69562893	Msa0517840:intron	35.0%
	TTCGTTCAACGACTTGAATT+TGG	-	chr3_4:69563626-69563645	Msa0517840:intron	35.0%
!!!	TGTTGATAATGCAGCTTTTC+GGG	-	chr3_4:69563678-69563697	Msa0517840:CDS	35.0%
!!	AGTTGGTGTAGGTTTTCATT+CGG	+	chr3_4:69563742-69563761	None:intergenic	35.0%
	AATACTTTATCCTTCTAGCC+TGG	+	chr3_4:69563805-69563824	None:intergenic	35.0%
	TAGATGAATAAGGACTTCCT+TGG	-	chr3_4:69563875-69563894	Msa0517840:CDS	35.0%
	GAAAATCTTGATCAACTCCA+AGG	+	chr3_4:69563895-69563914	None:intergenic	35.0%
!	TTGTGTCCATTTTCTGTACA+AGG	-	chr3_4:69563915-69563934	Msa0517840:CDS	35.0%
!	TGTGTCCATTTTCTGTACAA+GGG	-	chr3_4:69563916-69563935	Msa0517840:CDS	35.0%
	TAAAAACACAGTTCTCTTCG+CGG	+	chr3_4:69564006-69564025	None:intergenic	35.0%
	TCATCCAATAGGTTTGGATT+GGG	+	chr3_4:69564131-69564150	None:intergenic	35.0%
	TTCATCCAATAGGTTTGGAT+TGG	+	chr3_4:69564132-69564151	None:intergenic	35.0%
	ATGTCTTCATCCAATAGGTT+TGG	+	chr3_4:69564137-69564156	None:intergenic	35.0%
	TCACAACTTTCTTGCAATTG+TGG	-	chr3_4:69564306-69564325	Msa0517840:five_prime_UTR	35.0%
	CACAACTTTCTTGCAATTGT+GGG	-	chr3_4:69564307-69564326	Msa0517840:five_prime_UTR	35.0%
	ACAACTTTCTTGCAATTGTG+GGG	-	chr3_4:69564308-69564327	Msa0517840:five_prime_UTR	35.0%
!	CCTATGTTTTCTCTTCACCT+AGG	+	chr3_4:69560748-69560767	None:intergenic	40.0%
	CCTAGGTGAAGAGAAAACAT+AGG	-	chr3_4:69560745-69560764	Msa0517840:three_prime_UTR	40.0%
	CAAAGAGGTCATTGAGAGAA+AGG	+	chr3_4:69560885-69560904	None:intergenic	40.0%
	CTTTCTCTCAATGACCTCTT+TGG	-	chr3_4:69560883-69560902	Msa0517840:three_prime_UTR	40.0%
!	GAAGACAGAAACTTTTGCAG+AGG	+	chr3_4:69560929-69560948	None:intergenic	40.0%
	TTCTCTGTCAGATGTTCTTC+TGG	-	chr3_4:69560946-69560965	Msa0517840:three_prime_UTR	40.0%
	GCTAGACTTGTGTTAGAAGA+TGG	-	chr3_4:69561143-69561162	Msa0517840:three_prime_UTR	40.0%
!!	GCTAAATCATTTGGTGCTTC+AGG	-	chr3_4:69561179-69561198	Msa0517840:three_prime_UTR	40.0%
	GATTTGTCATCAGCACAAAC+TGG	+	chr3_4:69561389-69561408	None:intergenic	40.0%
	ATGCCAGTATTCCCAATATG+TGG	+	chr3_4:69561411-69561430	None:intergenic	40.0%
!	GGGAATACTGGCATCAATTT+TGG	-	chr3_4:69561417-69561436	Msa0517840:intron	40.0%
	AATATCCATCGCAAAGAGAC+AGG	+	chr3_4:69561675-69561694	None:intergenic	40.0%
	TGGAGATGTGTGAAGGATTT+GGG	-	chr3_4:69561712-69561731	Msa0517840:CDS	40.0%
	TGGGCGATTACTTAACTGAA+AGG	-	chr3_4:69561731-69561750	Msa0517840:CDS	40.0%
	CAGTGCTCAAAACACCAATA+AGG	+	chr3_4:69561915-69561934	None:intergenic	40.0%
	TGACAATTCCAAAACAGACG+AGG	-	chr3_4:69561933-69561952	Msa0517840:intron	40.0%
	ACAACTCTGCTGGAATTACA+TGG	-	chr3_4:69562207-69562226	Msa0517840:intron	40.0%
	AACTTCAAAGTTTCGGATGC+TGG	+	chr3_4:69562545-69562564	None:intergenic	40.0%
!	TTGCTGAAAAGTACACCATC+TGG	+	chr3_4:69562572-69562591	None:intergenic	40.0%
!	GATGGTGTACTTTTCAGCAA+TGG	-	chr3_4:69562572-69562591	Msa0517840:CDS	40.0%
!!!	ATTGGAAAGGTGCCTGTTTT+TGG	-	chr3_4:69562647-69562666	Msa0517840:intron	40.0%
!!!	GCCTGTTTTTGGAATTTGCA+TGG	-	chr3_4:69562658-69562677	Msa0517840:intron	40.0%
	CCCATGCAAATTCCAAAAAC+AGG	+	chr3_4:69562662-69562681	None:intergenic	40.0%
	ATGAAATTTGGTCACCATGG+AGG	-	chr3_4:69562725-69562744	Msa0517840:intron	40.0%
!	CCGTGTTGAAATTAGTTCTC+AGG	-	chr3_4:69562778-69562797	Msa0517840:CDS	40.0%
	GAATTCATTTGGTCTGATGG+GGG	-	chr3_4:69562875-69562894	Msa0517840:intron	40.0%
!	TGTCCATGAGTTGACACAAT+GGG	+	chr3_4:69563083-69563102	None:intergenic	40.0%
!	ATGTCCATGAGTTGACACAA+TGG	+	chr3_4:69563084-69563103	None:intergenic	40.0%
	CATGGACATTATTAGACCTG+TGG	-	chr3_4:69563095-69563114	Msa0517840:intron	40.0%
	TGAGTAACTTCTACTCCTTC+AGG	+	chr3_4:69563345-69563364	None:intergenic	40.0%
!	GAGACACAATCTTTTGAGCA+GGG	+	chr3_4:69563407-69563426	None:intergenic	40.0%
!	AGAGACACAATCTTTTGAGC+AGG	+	chr3_4:69563408-69563427	None:intergenic	40.0%
	AGCAGGATTATGTGTCTTGT+AGG	-	chr3_4:69563570-69563589	Msa0517840:intron	40.0%
!!!	GTAGGTTTTCATTCGGTGTT+TGG	+	chr3_4:69563735-69563754	None:intergenic	40.0%
	AATCCCCAATCCAAACCTAT+TGG	-	chr3_4:69564124-69564143	Msa0517840:CDS	40.0%
	CATCCAATAGGTTTGGATTG+GGG	+	chr3_4:69564130-69564149	None:intergenic	40.0%
	GCACAATGTCTTCATCCAAT+AGG	+	chr3_4:69564142-69564161	None:intergenic	40.0%
	CAACTTTCTTGCAATTGTGG+GGG	-	chr3_4:69564309-69564328	Msa0517840:five_prime_UTR	40.0%
	ACATAGGTTGCTGCTAACAC+TGG	-	chr3_4:69560761-69560780	Msa0517840:three_prime_UTR	45.0%
	GTCATTGAGAGAAAGGGTGA+AGG	+	chr3_4:69560878-69560897	None:intergenic	45.0%
!	CAATGACCTCTTTGGTTCCA+AGG	-	chr3_4:69560891-69560910	Msa0517840:three_prime_UTR	45.0%
!!	GAAACTTTTGCAGAGGTGTG+TGG	+	chr3_4:69560922-69560941	None:intergenic	45.0%
	ATGTGTGTGTTCCATTCCTC+AGG	-	chr3_4:69561328-69561347	Msa0517840:CDS	45.0%
	ACAAACTGGCCAGCATAACT+AGG	+	chr3_4:69561375-69561394	None:intergenic	45.0%
	TCATTGTCTGACAGATGACG+AGG	-	chr3_4:69561518-69561537	Msa0517840:intron	45.0%
	CTTCACACATCTCCAGTTTG+AGG	+	chr3_4:69561707-69561726	None:intergenic	45.0%
	CTCAAACTGGAGATGTGTGA+AGG	-	chr3_4:69561705-69561724	Msa0517840:CDS	45.0%
	CTGGAGATGTGTGAAGGATT+TGG	-	chr3_4:69561711-69561730	Msa0517840:CDS	45.0%
	GGGCAATCACACGTAGATTA+CGG	-	chr3_4:69561878-69561897	Msa0517840:intron	45.0%
	GGCAATCACACGTAGATTAC+GGG	-	chr3_4:69561879-69561898	Msa0517840:intron	45.0%
	ACACGTAGATTACGGGAAGA+TGG	-	chr3_4:69561886-69561905	Msa0517840:intron	45.0%
	TCTGTGATTGACAACTCTGC+TGG	-	chr3_4:69562197-69562216	Msa0517840:intron	45.0%
	CGTGCAAGTCACCATATGAA+TGG	-	chr3_4:69562274-69562293	Msa0517840:intron	45.0%
	CTTAGGCGTCTAGCATCATA+TGG	-	chr3_4:69562488-69562507	Msa0517840:CDS	45.0%
	TCACTGTTGTGCCATCTACA+TGG	-	chr3_4:69562519-69562538	Msa0517840:CDS	45.0%
!	GTACTTTTCAGCAATGGTCC+TGG	-	chr3_4:69562578-69562597	Msa0517840:CDS	45.0%
	TGCATGGGACATCAATTGCT+AGG	-	chr3_4:69562674-69562693	Msa0517840:intron	45.0%
	GAACAGGATGATTTCCTCCA+TGG	+	chr3_4:69562742-69562761	None:intergenic	45.0%
	TTCATAGAAGGGTCAGAACC+TGG	-	chr3_4:69562943-69562962	Msa0517840:intron	45.0%
	GGCACTATCATAGTGAGTTC+TGG	+	chr3_4:69563000-69563019	None:intergenic	45.0%
!	TCCATGAGTTGACACAATGG+GGG	+	chr3_4:69563081-69563100	None:intergenic	45.0%
!	GTCCATGAGTTGACACAATG+GGG	+	chr3_4:69563082-69563101	None:intergenic	45.0%
	CTCAATGACAGTAGTTGTGC+CGG	-	chr3_4:69563372-69563391	Msa0517840:intron	45.0%
	CACCGTTGTCAGAATCATGA+GGG	+	chr3_4:69563458-69563477	None:intergenic	45.0%
	TCACCGTTGTCAGAATCATG+AGG	+	chr3_4:69563459-69563478	None:intergenic	45.0%
!	ACACTGATTGCAGTTGGTGT+AGG	+	chr3_4:69563753-69563772	None:intergenic	45.0%
	CTTTATCCTTCTAGCCTGGT+GGG	+	chr3_4:69563801-69563820	None:intergenic	45.0%
	ACTTTATCCTTCTAGCCTGG+TGG	+	chr3_4:69563802-69563821	None:intergenic	45.0%
	GCTAGCCCTTGTACAGAAAA+TGG	+	chr3_4:69563924-69563943	None:intergenic	45.0%
	CTCGTAGCCTTCATTGAAGA+AGG	-	chr3_4:69563960-69563979	Msa0517840:intron	45.0%
	TGTGACCACTTGCAGCTAAA+GGG	+	chr3_4:69564291-69564310	None:intergenic	45.0%
	TTGTGACCACTTGCAGCTAA+AGG	+	chr3_4:69564292-69564311	None:intergenic	45.0%
	TTTCTTGCAATTGTGGGGGT+TGG	-	chr3_4:69564313-69564332	Msa0517840:five_prime_UTR	45.0%
!	GGTTGCTGCTAACACTGGTT+TGG	-	chr3_4:69560766-69560785	Msa0517840:three_prime_UTR	50.0%
!!	ACTTTTGCAGAGGTGTGTGG+CGG	+	chr3_4:69560919-69560938	None:intergenic	50.0%
!!	TAGGTAATGGGGAAAGGCCA+TGG	-	chr3_4:69561108-69561127	Msa0517840:three_prime_UTR	50.0%
	TCAGCACAGTGCTTCCTTTC+CGG	-	chr3_4:69561543-69561562	Msa0517840:intron	50.0%
	CTCTTCCTGTCTCTTTGCGA+TGG	-	chr3_4:69561667-69561686	Msa0517840:CDS	50.0%
!	TGGGAGTTTAGCTCCAGTGA+AGG	-	chr3_4:69562318-69562337	Msa0517840:CDS	50.0%
!	GGGAGTTTAGCTCCAGTGAA+GGG	-	chr3_4:69562319-69562338	Msa0517840:CDS	50.0%
	AGTATCTCTAGGCCCTTCAC+TGG	+	chr3_4:69562334-69562353	None:intergenic	50.0%
	ACGGCATAAGGAACAGCAGA+AGG	+	chr3_4:69562608-69562627	None:intergenic	50.0%
	CAATTGCTAGGCCAGGCTTT+AGG	-	chr3_4:69562686-69562705	Msa0517840:intron	50.0%
	AGGTTGTACCTCCTAAAGCC+TGG	+	chr3_4:69562700-69562719	None:intergenic	50.0%
	CCAGTTCGAAGGTTACGAAC+AGG	+	chr3_4:69562758-69562777	None:intergenic	50.0%
	CCTGTTCGTAACCTTCGAAC+TGG	-	chr3_4:69562755-69562774	Msa0517840:CDS	50.0%
	TTTCAACACGGCCAGTTCGA+AGG	+	chr3_4:69562769-69562788	None:intergenic	50.0%
	TTCATTTGGTCTGATGGGGG+AGG	-	chr3_4:69562878-69562897	Msa0517840:intron	50.0%
	TCATTTGGTCTGATGGGGGA+GGG	-	chr3_4:69562879-69562898	Msa0517840:intron	50.0%
	TCTTCTCAAGACAAGGGACC+AGG	+	chr3_4:69562964-69562983	None:intergenic	50.0%
!	CTCACTATGATAGTGCCTCG+TGG	-	chr3_4:69563003-69563022	Msa0517840:intron	50.0%
!	TGGGGGCATAACAGAACTGA+GGG	+	chr3_4:69563064-69563083	None:intergenic	50.0%
!	ATGGGGGCATAACAGAACTG+AGG	+	chr3_4:69563065-69563084	None:intergenic	50.0%
	CAGGGTCAACTGCATAGTTG+TGG	+	chr3_4:69563314-69563333	None:intergenic	50.0%
	TTCTACTCCTTCAGGCAGTG+TGG	+	chr3_4:69563337-69563356	None:intergenic	50.0%
	TTGAGCAGGGAAAGCAAGAC+CGG	+	chr3_4:69563394-69563413	None:intergenic	50.0%
	TTGTCAGAATCATGAGGGCC+AGG	+	chr3_4:69563453-69563472	None:intergenic	50.0%
	GGCCCTCATGATTCTGACAA+CGG	-	chr3_4:69563453-69563472	Msa0517840:intron	50.0%
	TGCATCCCTTTAGCTGCAAG+TGG	-	chr3_4:69564283-69564302	Msa0517840:five_prime_UTR	50.0%
	TTGCAATTGTGGGGGTTGGA+TGG	-	chr3_4:69564317-69564336	Msa0517840:five_prime_UTR	50.0%
	TGCAATTGTGGGGGTTGGAT+GGG	-	chr3_4:69564318-69564337	Msa0517840:five_prime_UTR	50.0%
	GGTGCTTCAGGAACCCAAGT+TGG	-	chr3_4:69561191-69561210	Msa0517840:three_prime_UTR	55.0%
!	CGGGAAGATGGCAGCCTTAT+TGG	-	chr3_4:69561898-69561917	Msa0517840:intron	55.0%
	TCGGATGCTGGCCATGTAGA+TGG	+	chr3_4:69562533-69562552	None:intergenic	55.0%
	GGAACAGCAGAAGGATCTCC+AGG	+	chr3_4:69562599-69562618	None:intergenic	55.0%
	GGGACATCAATTGCTAGGCC+AGG	-	chr3_4:69562679-69562698	Msa0517840:intron	55.0%
!!	TTGCTAGGCCAGGCTTTAGG+AGG	-	chr3_4:69562689-69562708	Msa0517840:intron	55.0%
	GCCCCCATTGTGTCAACTCA+TGG	-	chr3_4:69563077-69563096	Msa0517840:intron	55.0%
!	TACCACCCTGAAGCATCTCC+TGG	-	chr3_4:69563432-69563451	Msa0517840:intron	55.0%
	AAGCTAGCTGCTATCCCACC+AGG	-	chr3_4:69563784-69563803	Msa0517840:intron	55.0%
	TGCTATCCCACCAGGCTAGA+AGG	-	chr3_4:69563792-69563811	Msa0517840:intron	55.0%
!	TACAAGGGCTAGCAGCCGAT+TGG	-	chr3_4:69563931-69563950	Msa0517840:CDS	55.0%
	GGCTACGAGCCTATGCCAAT+CGG	+	chr3_4:69563949-69563968	None:intergenic	55.0%
	GCAATTGTGGGGGTTGGATG+GGG	-	chr3_4:69564319-69564338	Msa0517840:five_prime_UTR	55.0%
	ACTGCGGCACTATAGCGCAG+CGG	-	chr3_4:69562056-69562075	Msa0517840:intron	60.0%
	CTCCTTCAGGCAGTGTGGCA+GGG	+	chr3_4:69563332-69563351	None:intergenic	60.0%
	ACTCCTTCAGGCAGTGTGGC+AGG	+	chr3_4:69563333-69563352	None:intergenic	60.0%
	GAGGGCCAGGAGATGCTTCA+GGG	+	chr3_4:69563440-69563459	None:intergenic	60.0%
	TGAGGGCCAGGAGATGCTTC+AGG	+	chr3_4:69563441-69563460	None:intergenic	60.0%
	GGCTAGCAGCCGATTGGCAT+AGG	-	chr3_4:69563937-69563956	Msa0517840:CDS	60.0%
	GCGGCGCCTTGGAACCAAAG+AGG	+	chr3_4:69560900-69560919	None:intergenic	65.0%
	GACCCTGCCACACTGCCTGA+AGG	-	chr3_4:69563327-69563346	Msa0517840:CDS	65.0%
	GGCCAGGAGATGCTTCAGGG+TGG	+	chr3_4:69563437-69563456	None:intergenic	65.0%
!	AGAGGTGTGTGGCGGCGCCT+TGG	+	chr3_4:69560911-69560930	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	69560726	69564361	69560726	ID=Msa0517840;Name=Msa0517840
chr3_4	mRNA	69560726	69564361	69560726	ID=Msa0517840-mRNA-1;Parent=Msa0517840;Name=Msa0517840-mRNA-1;_AED=0.11;_eAED=0.11;_QI=130\|0.88\|0.8\|1\|0.88\|0.9\|10\|587\|401
chr3_4	exon	69560726	69561395	69560726	ID=Msa0517840-mRNA-1:exon:18536;Parent=Msa0517840-mRNA-1
chr3_4	exon	69561613	69561778	69561613	ID=Msa0517840-mRNA-1:exon:18535;Parent=Msa0517840-mRNA-1
chr3_4	exon	69562288	69562635	69562288	ID=Msa0517840-mRNA-1:exon:18534;Parent=Msa0517840-mRNA-1
chr3_4	exon	69562728	69562816	69562728	ID=Msa0517840-mRNA-1:exon:18533;Parent=Msa0517840-mRNA-1
chr3_4	exon	69563168	69563224	69563168	ID=Msa0517840-mRNA-1:exon:18532;Parent=Msa0517840-mRNA-1
chr3_4	exon	69563318	69563385	69563318	ID=Msa0517840-mRNA-1:exon:18531;Parent=Msa0517840-mRNA-1
chr3_4	exon	69563495	69563555	69563495	ID=Msa0517840-mRNA-1:exon:18530;Parent=Msa0517840-mRNA-1
chr3_4	exon	69563649	69563737	69563649	ID=Msa0517840-mRNA-1:exon:18529;Parent=Msa0517840-mRNA-1
chr3_4	exon	69563844	69563976	69563844	ID=Msa0517840-mRNA-1:exon:18528;Parent=Msa0517840-mRNA-1
chr3_4	exon	69564120	69564361	69564120	ID=Msa0517840-mRNA-1:exon:18527;Parent=Msa0517840-mRNA-1
chr3_4	five_prime_UTR	69564232	69564361	69564232	ID=Msa0517840-mRNA-1:five_prime_utr;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69564120	69564231	69564120	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69563844	69563976	69563844	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69563649	69563737	69563649	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69563495	69563555	69563495	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69563318	69563385	69563318	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69563168	69563224	69563168	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69562728	69562816	69562728	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69562288	69562635	69562288	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69561613	69561778	69561613	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	CDS	69561313	69561395	69561313	ID=Msa0517840-mRNA-1:cds;Parent=Msa0517840-mRNA-1
chr3_4	three_prime_UTR	69560726	69561312	69560726	ID=Msa0517840-mRNA-1:three_prime_utr;Parent=Msa0517840-mRNA-1

Gene Sequence

>Msa0517840

ATGGCAACAAAAGCTTTGGCCTTCACCCTTTCTCTCAATGACCTCTTTGGTTCCAAGGCGCCGCCACACACCTCTGCAAAAGTTTCTGTCTTCTCTGTCAGATGTTCTTCTGGTAATGGGGAAAGGCCATGGAAAAATTCAGATGCTAGACTTGTGTTAGAAGATGGTTCAATTTGGAAAGCTAAATCATTTGGTGCTTCAGGAACCCAAGTTGGTGAAGTTGTTTTCAATACATCATTGACTGGGTATCAAGAAATTTTAACTGATCCTAGTTATGCTGGCCAGTTTGTGCTGATGACAAATCCACATATTGGGAATACTGGCATCAATTTTGATGACGAGGAATCAGCACAGTGCTTCCTTTCCGGTTTAGTAATTAGAAGTTTGAGTATCAGTACCTCAAACTGGAGATGTGTGAAGGATTTGGGCGATTACTTAACTGAAAGGAATGTCATGGGAATTTATGATGTTGATACAAGGGCAATCACACGTAGATTACGGGAAGATGGCAGCCTTATTGTATCGTGCAAGTCACCATATGAATGGATTGATAGAACAAAGGAAGAATGGGAGTTTAGCTCCAGTGAAGGGCCTAGAGATACTTTCCATGTAGTTGCATACGATTTTGGAATCAAGCATAATATACTTAGGCGTCTAGCATCATATGGCTGTAAAATCACTGTTGTGCCATCTACATGGCCAGCATCCGAAACTTTGAAGTTAAATCCAGATGGTGTACTTTTCAGCAATGGTCCTGGAGATCCTTCTGCTGTTCCTTATGCCGTTGAAACAGTAAAGAATATCATTGGAAAGGTGCCTGTTTTTGGAATTTGCATGGGACATCAATTGCTAGGCCAGGCTTTAGGAGGTACAACCTACAAGATGAAATTTGGTCACCATGGAGGAAATCATCCTGTTCGTAACCTTCGAACTGGCCGTGTTGAAATTAGTTCTCAGAACCACAACTATGCAGTTGACCCTGCCACACTGCCTGAAGGAGTAGAAGTTACTCATATCAATCTCAATGACAGTAGTTGTGCCGGTCTTGCTTTCCCTGCTCAAAAGATTGTGTCTCTTCAATACCACCCTGAAGCATCTCCTGGCCCTCATGATTCTGACAACGCTTTTCGGGAGTTTATAGAGCTAATGAAGCATGAGAAAAACCAAACACCGAATGAAAACCTACACCAACTGCAATCAGTGTAA

Protein sequence

>Msa0517840

MATKALAFTLSLNDLFGSKAPPHTSAKVSVFSVRCSSGNGERPWKNSDARLVLEDGSIWKAKSFGASGTQVGEVVFNTSLTGYQEILTDPSYAGQFVLMTNPHIGNTGINFDDEESAQCFLSGLVIRSLSISTSNWRCVKDLGDYLTERNVMGIYDVDTRAITRRLREDGSLIVSCKSPYEWIDRTKEEWEFSSSEGPRDTFHVVAYDFGIKHNILRRLASYGCKITVVPSTWPASETLKLNPDGVLFSNGPGDPSAVPYAVETVKNIIGKVPVFGICMGHQLLGQALGGTTYKMKFGHHGGNHPVRNLRTGRVEISSQNHNYAVDPATLPEGVEVTHINLNDSSCAGLAFPAQKIVSLQYHPEASPGPHDSDNAFREFIELMKHEKNQTPNENLHQLQSV*