AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0520530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0520530 MtrunA17_Chr3g0136301 95.197 229 9 1 1 227 1 229 2.22e-162 447
Msa0520530 MtrunA17_Chr8g0336911 45.026 191 103 2 32 221 30 219 1.72e-54 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0520530 AT1G02180.1 42.529 174 96 4 32 203 29 200 3.57e-43 145

Find 71 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAGGGTCTCCTTATTTC+TGG 0.118257 3_4:+73089942 Msa0520530:CDS
CAAGATGCATTTGGACATTA+TGG 0.174203 3_4:+73090390 Msa0520530:three_prime_UTR
TCGTATAACTGGTCGTTTCT+TGG 0.250063 3_4:+73089793 Msa0520530:CDS
GCTTCAGCAAATTGAGATTC+AGG 0.261524 3_4:-73089731 None:intergenic
AGCTCTTCTCGTTTCTTATC+AGG 0.274033 3_4:-73089862 None:intergenic
CGTATAACTGGTCGTTTCTT+GGG 0.276791 3_4:+73089794 Msa0520530:CDS
ATGTACCCTAGCCCTGAAAA+AGG 0.292502 3_4:+73090414 Msa0520530:three_prime_UTR
TTCATCCTTTCCTTCCATCT+TGG 0.302387 3_4:+73087606 Msa0520530:CDS
ATGCCTTAACACCACAGTTT+GGG 0.323486 3_4:-73089760 None:intergenic
AACAACACACACCAGAAATA+AGG 0.333485 3_4:-73089953 None:intergenic
GATGCCTTAACACCACAGTT+TGG 0.357391 3_4:-73089761 None:intergenic
ATTGGAATTCAAGATGCATT+TGG 0.358573 3_4:+73090381 Msa0520530:three_prime_UTR
TCAACTCTTGCTCGTATAAC+TGG 0.364017 3_4:+73089782 Msa0520530:CDS
TCAAAAGTAAATGTGCTGTT+AGG 0.374585 3_4:-73089989 None:intergenic
TGCCCCAAACTGTGGTGTTA+AGG 0.376413 3_4:+73089757 Msa0520530:CDS
GCATATGAAACATGTGATAT+TGG 0.378061 3_4:+73090306 Msa0520530:three_prime_UTR
GCTTCAGTTTCAATTGCAAT+GGG 0.382192 3_4:+73090118 Msa0520530:CDS
CAAGTTGGTGCTGCTGTAAC+CGG 0.391700 3_4:+73089914 Msa0520530:CDS
ACAAAACCAGGTTGCTATAG+TGG 0.403030 3_4:+73090031 Msa0520530:CDS
TGCTTCAGTTTCAATTGCAA+TGG 0.413253 3_4:+73090117 Msa0520530:CDS
TGCTTTGATTGTTATACGAT+TGG 0.413715 3_4:+73090363 Msa0520530:three_prime_UTR
TCTCGCCCTACAGTACATTA+AGG 0.413855 3_4:+73089658 Msa0520530:CDS
TTCCATCTTGGTGCTTCTAC+CGG 0.414909 3_4:+73087618 Msa0520530:CDS
AAAAGTTTGTTAGACACGTT+TGG 0.418307 3_4:+73087479 None:intergenic
TCATCTTTATTTGACAATCC+CGG 0.454811 3_4:+73089626 Msa0520530:CDS
TGTGCAGTGGTGCTCATGTT+TGG 0.457962 3_4:+73090065 Msa0520530:CDS
ATATCACATGTTTCATATGC+AGG 0.471229 3_4:-73090303 None:intergenic
TAGGGCGAGACATGCGAGCC+CGG 0.474850 3_4:-73089645 None:intergenic
GCAGCTACTAACTTGTCTGC+AGG 0.476484 3_4:-73089572 None:intergenic
CATCTTTATTTGACAATCCC+GGG 0.500468 3_4:+73089627 Msa0520530:CDS
TGGTGTGTGTTGTTTAGTAA+TGG 0.500489 3_4:+73089962 Msa0520530:CDS
GGTGATTGTGGTGCTGTGGG+TGG 0.502206 3_4:+73089689 Msa0520530:CDS
GGTATGCCTTAATGTACTGT+AGG 0.503759 3_4:-73089664 None:intergenic
CAGGGTGATTGTGGTGCTGT+GGG 0.510763 3_4:+73089686 Msa0520530:CDS
CTTCAGCAAATTGAGATTCA+GGG 0.511447 3_4:-73089730 None:intergenic
CGAAAACAGAACTGCTCACA+AGG 0.516931 3_4:+73089601 Msa0520530:CDS
GTGCATGGACATACTTAGTC+TGG 0.524594 3_4:-73089820 None:intergenic
GTATAACTGGTCGTTTCTTG+GGG 0.529253 3_4:+73089795 Msa0520530:CDS
CCAGGGTGATTGTGGTGCTG+TGG 0.539111 3_4:+73089685 Msa0520530:CDS
AATAGCCACATCACACTGAC+TGG 0.539928 3_4:-73090091 None:intergenic
AAGGCATACCAGGGTGATTG+TGG 0.548506 3_4:+73089677 Msa0520530:CDS
ACAGTACATTAAGGCATACC+AGG 0.557681 3_4:+73089667 Msa0520530:CDS
CAGAAAACGCCTCAGGTGCA+TGG 0.559426 3_4:-73089835 None:intergenic
CACCGGTAGAAGCACCAAGA+TGG 0.565056 3_4:-73087620 None:intergenic
GCTGCTGTAACCGGTACTGA+TGG 0.569000 3_4:+73089923 Msa0520530:CDS
AGTAAGAATCACACTCAAGT+TGG 0.576187 3_4:+73089899 Msa0520530:CDS
AGGGCGAGACATGCGAGCCC+GGG 0.579446 3_4:-73089644 None:intergenic
TGCCTTAACACCACAGTTTG+GGG 0.580489 3_4:-73089759 None:intergenic
GCTGTAACCGGTACTGATGG+AGG 0.581331 3_4:+73089926 Msa0520530:CDS
AGGATTTCAGAAAACGCCTC+AGG 0.582162 3_4:-73089842 None:intergenic
CCTCTGTAGTGACAAAATGA+AGG 0.583962 3_4:-73090186 None:intergenic
AGTTTGTTAGACACGTTTGG+TGG 0.590169 3_4:+73087482 None:intergenic
AAGCACCAAGATGGAAGGAA+AGG 0.596123 3_4:-73087611 None:intergenic
AGGAGACCCTCCATCAGTAC+CGG 0.597918 3_4:-73089933 None:intergenic
GTATGCCTTAATGTACTGTA+GGG 0.599616 3_4:-73089663 None:intergenic
TTCAGCAAATTGAGATTCAG+GGG 0.606803 3_4:-73089729 None:intergenic
GGTAGAAGCACCAAGATGGA+AGG 0.607147 3_4:-73087616 None:intergenic
GGACTTACTTTGAGTAGCAC+CGG 0.613594 3_4:-73087637 None:intergenic
GGTCACCAGTCAGTGTGATG+TGG 0.613666 3_4:+73090086 Msa0520530:CDS
CAGCTACTAACTTGTCTGCA+GGG 0.614123 3_4:-73089571 None:intergenic
CTTCAGTTTCAATTGCAATG+GGG 0.618866 3_4:+73090119 Msa0520530:CDS
CTGTAACCGGTACTGATGGA+GGG 0.623210 3_4:+73089927 Msa0520530:CDS
AAATAACAAAATCAAAACAA+AGG 0.645136 3_4:-73090241 None:intergenic
TTAGCTCCACTATAGCAACC+TGG 0.653166 3_4:-73090037 None:intergenic
TCAGCAAATTGAGATTCAGG+GGG 0.657704 3_4:-73089728 None:intergenic
CCACAGCACCACAATCACCC+TGG 0.672377 3_4:-73089685 None:intergenic
GTGGCTAAAGAAACAAAACC+AGG 0.685787 3_4:+73090019 Msa0520530:CDS
TAAGTATGTCCATGCACCTG+AGG 0.686474 3_4:+73089826 Msa0520530:CDS
GTGTCTGCTTCAATGTAGTG+AGG 0.687007 3_4:-73087510 None:intergenic
CAGTACATTAAGGCATACCA+GGG 0.710618 3_4:+73089668 Msa0520530:CDS
GGAGCTAATGATGTGTGCAG+TGG 0.715652 3_4:+73090052 Msa0520530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCATTGATTATATATA+TGG + chr3_4:73089325-73089344 Msa0520530:intron 10.0%
!!! AATAAAAAAAAGACTTGTTT+TGG - chr3_4:73087661-73087680 None:intergenic 15.0%
!!! TTTATTTTATATCTCAGATT+TGG + chr3_4:73087675-73087694 Msa0520530:intron 15.0%
!! CATTTGTCATTAAAAAAAAT+TGG + chr3_4:73088304-73088323 Msa0520530:intron 15.0%
!! AGAAAATAAATATAACTATG+AGG - chr3_4:73089441-73089460 None:intergenic 15.0%
!! AAATAACAAAATCAAAACAA+AGG - chr3_4:73090244-73090263 None:intergenic 15.0%
!! CTATAACTCATTAAAGTTTA+TGG + chr3_4:73088041-73088060 Msa0520530:intron 20.0%
!! CATTAAAAAAAATTGGTTGT+AGG + chr3_4:73088311-73088330 Msa0520530:intron 20.0%
!! GACAATATAAAATGAAATCA+AGG - chr3_4:73088406-73088425 None:intergenic 20.0%
!! AAAAAAATTGAACTGTTATG+TGG + chr3_4:73088587-73088606 Msa0520530:intron 20.0%
!! AAATATGTAACCTTAATCAA+AGG - chr3_4:73089301-73089320 None:intergenic 20.0%
!! ATAAATATAACTATGAGGTA+GGG - chr3_4:73089436-73089455 None:intergenic 20.0%
!! AATAAATATAACTATGAGGT+AGG - chr3_4:73089437-73089456 None:intergenic 20.0%
!!! TGAATTTTTACTTGATTTAG+TGG + chr3_4:73090163-73090182 Msa0520530:three_prime_UTR 20.0%
! TATGTAAAATGAGTTTGATC+TGG + chr3_4:73087716-73087735 Msa0520530:intron 25.0%
! TCTTGCAAGTTGTATTAAAA+TGG + chr3_4:73087885-73087904 Msa0520530:intron 25.0%
! TGAAAGTTCCAAATAAGATA+CGG - chr3_4:73087928-73087947 None:intergenic 25.0%
!! ATTTGGAACTTTCACTATAA+TGG + chr3_4:73087934-73087953 Msa0520530:intron 25.0%
! TATGTTAGTTAGTCACAAAT+TGG + chr3_4:73088158-73088177 Msa0520530:intron 25.0%
! TTAAACAATAAGCATCAAAC+GGG - chr3_4:73088485-73088504 None:intergenic 25.0%
! CTTAAACAATAAGCATCAAA+CGG - chr3_4:73088486-73088505 None:intergenic 25.0%
! TTGTTTAAGAGCTAATTATG+TGG + chr3_4:73088497-73088516 Msa0520530:intron 25.0%
! TTTCAACAAAATTAATCCAG+GGG - chr3_4:73088526-73088545 None:intergenic 25.0%
! CTTTCAACAAAATTAATCCA+GGG - chr3_4:73088527-73088546 None:intergenic 25.0%
! TCTTTCAACAAAATTAATCC+AGG - chr3_4:73088528-73088547 None:intergenic 25.0%
!! ATGACTTTTGTAGTGAAATT+AGG + chr3_4:73088717-73088736 Msa0520530:intron 25.0%
! TGAAATTAGGACTAAACTTA+TGG + chr3_4:73088730-73088749 Msa0520530:intron 25.0%
!!! TGAATATCTAGCTTTCATTA+TGG + chr3_4:73088860-73088879 Msa0520530:intron 25.0%
! AGACAAACTATAACAAATGT+TGG - chr3_4:73089045-73089064 None:intergenic 25.0%
!!! ACATTTGTTATAGTTTGTCT+AGG + chr3_4:73089045-73089064 Msa0520530:intron 25.0%
! TAATCAAAGGATAAAAGGAA+AGG - chr3_4:73089288-73089307 None:intergenic 25.0%
!! TTCCTTTTATCCTTTGATTA+AGG + chr3_4:73089288-73089307 Msa0520530:intron 25.0%
! AACCTTAATCAAAGGATAAA+AGG - chr3_4:73089293-73089312 None:intergenic 25.0%
! GTAAAAATTCATGATCACAA+TGG - chr3_4:73090154-73090173 None:intergenic 25.0%
!!! TGATTTTGTTATTTGTTGAG+AGG + chr3_4:73090250-73090269 Msa0520530:three_prime_UTR 25.0%
!! AAATTGTTGCATAAGCTTTT+TGG + chr3_4:73090441-73090460 Msa0520530:three_prime_UTR 25.0%
ATTACTGTGAAATGCTACAT+TGG - chr3_4:73087804-73087823 None:intergenic 30.0%
AGGAAAAAGAGAACAAGTTA+AGG + chr3_4:73087973-73087992 Msa0520530:intron 30.0%
ATTGTCCAAATCAAGAATCA+CGG - chr3_4:73088004-73088023 None:intergenic 30.0%
!!! TTATGGTCTTTGTTTTAGTG+TGG + chr3_4:73088058-73088077 Msa0520530:intron 30.0%
TTGTGACTAACTAACATAAG+TGG - chr3_4:73088156-73088175 None:intergenic 30.0%
TTTATAACTGTATACGCTTC+CGG + chr3_4:73088360-73088379 Msa0520530:intron 30.0%
! CATTTTATATTGTCTCTGCT+CGG + chr3_4:73088412-73088431 Msa0520530:intron 30.0%
AATTGAACTGTTATGTGGTT+AGG + chr3_4:73088592-73088611 Msa0520530:intron 30.0%
ACAAAAGTCATAACTTTGCA+TGG - chr3_4:73088708-73088727 None:intergenic 30.0%
AAACTTATGGAAGCCTTAAA+TGG + chr3_4:73088743-73088762 Msa0520530:intron 30.0%
! GGATTTAGGAATTTGAGATT+TGG + chr3_4:73088802-73088821 Msa0520530:intron 30.0%
ACAGAGTTTAGTGTATACAA+AGG + chr3_4:73088921-73088940 Msa0520530:intron 30.0%
CAGAGTTTAGTGTATACAAA+GGG + chr3_4:73088922-73088941 Msa0520530:intron 30.0%
AAGATAAATAAAACCGTTGC+TGG + chr3_4:73088955-73088974 Msa0520530:intron 30.0%
ACAATGATAGAAGCAATATC+AGG - chr3_4:73089008-73089027 None:intergenic 30.0%
AATTTAGAGAACACCAAAGT+GGG - chr3_4:73089503-73089522 None:intergenic 30.0%
TAATTTAGAGAACACCAAAG+TGG - chr3_4:73089504-73089523 None:intergenic 30.0%
! TCATCTTTATTTGACAATCC+CGG + chr3_4:73089626-73089645 Msa0520530:CDS 30.0%
! TCAAAAGTAAATGTGCTGTT+AGG - chr3_4:73089992-73090011 None:intergenic 30.0%
!! TAACAGCACATTTACTTTTG+AGG + chr3_4:73089991-73090010 Msa0520530:CDS 30.0%
!! AACAGCACATTTACTTTTGA+GGG + chr3_4:73089992-73090011 Msa0520530:CDS 30.0%
ATATCACATGTTTCATATGC+AGG - chr3_4:73090306-73090325 None:intergenic 30.0%
GCATATGAAACATGTGATAT+TGG + chr3_4:73090306-73090325 Msa0520530:three_prime_UTR 30.0%
! CTCTTGTAATTTTCTCATCT+AGG + chr3_4:73090329-73090348 Msa0520530:three_prime_UTR 30.0%
TGCTTTGATTGTTATACGAT+TGG + chr3_4:73090363-73090382 Msa0520530:three_prime_UTR 30.0%
ATTGGAATTCAAGATGCATT+TGG + chr3_4:73090381-73090400 Msa0520530:three_prime_UTR 30.0%
! GAACACAGTGTGAAAGTTTT+AGG - chr3_4:73087579-73087598 None:intergenic 35.0%
! AATTCTGCTAGCTGTAGTTT+TGG + chr3_4:73087821-73087840 Msa0520530:intron 35.0%
AGAACTGTCCGTATCTTATT+TGG + chr3_4:73087917-73087936 Msa0520530:intron 35.0%
TGGAACTTTCACTATAATGG+TGG + chr3_4:73087937-73087956 Msa0520530:intron 35.0%
ATATCCCGTGATTCTTGATT+TGG + chr3_4:73087996-73088015 Msa0520530:intron 35.0%
TTGTCCAAATCAAGAATCAC+GGG - chr3_4:73088003-73088022 None:intergenic 35.0%
GAGTTATAGAGACGAAGAAA+TGG - chr3_4:73088030-73088049 None:intergenic 35.0%
!! GTTTTAGTGTGGAGTTTCAT+TGG + chr3_4:73088069-73088088 Msa0520530:intron 35.0%
!! AGTTCAACTGGTTTGAGTTA+AGG + chr3_4:73088210-73088229 Msa0520530:intron 35.0%
!! GTTCAACTGGTTTGAGTTAA+GGG + chr3_4:73088211-73088230 Msa0520530:intron 35.0%
TGAGTTAAGGGATAAAGAGT+TGG + chr3_4:73088223-73088242 Msa0520530:intron 35.0%
! ACGTGGACATAGTTCATTTT+TGG - chr3_4:73088440-73088459 None:intergenic 35.0%
CTAGAGCTAGAAACATAGAT+AGG - chr3_4:73088566-73088585 None:intergenic 35.0%
!! CTATCTATGTTTCTAGCTCT+AGG + chr3_4:73088564-73088583 Msa0520530:intron 35.0%
AACAGAAGTCTCATCTTCAA+AGG - chr3_4:73088658-73088677 None:intergenic 35.0%
AAAGTCATAACTTTGCATGG+TGG - chr3_4:73088705-73088724 None:intergenic 35.0%
TACAAAGAGGTCACCATTTA+AGG - chr3_4:73088759-73088778 None:intergenic 35.0%
TATCTGTGTCAAGTACAAAG+AGG - chr3_4:73088772-73088791 None:intergenic 35.0%
!! GATAGAGAGTACTAGGATTT+AGG + chr3_4:73088788-73088807 Msa0520530:intron 35.0%
TAAGAGTGGAGAAAATGTTG+TGG + chr3_4:73088832-73088851 Msa0520530:intron 35.0%
TATACACTAAACTCTGTCTC+TGG - chr3_4:73088918-73088937 None:intergenic 35.0%
ATGATAGAAGCAATATCAGG+AGG - chr3_4:73089005-73089024 None:intergenic 35.0%
TGAGTTTCTCAACAGATTCT+CGG - chr3_4:73089111-73089130 None:intergenic 35.0%
AAAGTAGGCTGTTTATTGTG+AGG - chr3_4:73089358-73089377 None:intergenic 35.0%
! CACAATAAACAGCCTACTTT+TGG + chr3_4:73089358-73089377 Msa0520530:intron 35.0%
!! TTTTTCAGTGAGCCAAAAGT+AGG - chr3_4:73089373-73089392 None:intergenic 35.0%
! CATCTTTATTTGACAATCCC+GGG + chr3_4:73089627-73089646 Msa0520530:CDS 35.0%
GTATGCCTTAATGTACTGTA+GGG - chr3_4:73089666-73089685 None:intergenic 35.0%
TTCAGCAAATTGAGATTCAG+GGG - chr3_4:73089732-73089751 None:intergenic 35.0%
CTTCAGCAAATTGAGATTCA+GGG - chr3_4:73089733-73089752 None:intergenic 35.0%
AGTAAGAATCACACTCAAGT+TGG + chr3_4:73089899-73089918 Msa0520530:CDS 35.0%
AACAACACACACCAGAAATA+AGG - chr3_4:73089956-73089975 None:intergenic 35.0%
!! TGGTGTGTGTTGTTTAGTAA+TGG + chr3_4:73089962-73089981 Msa0520530:CDS 35.0%
TGCTTCAGTTTCAATTGCAA+TGG + chr3_4:73090117-73090136 Msa0520530:CDS 35.0%
GCTTCAGTTTCAATTGCAAT+GGG + chr3_4:73090118-73090137 Msa0520530:CDS 35.0%
CTTCAGTTTCAATTGCAATG+GGG + chr3_4:73090119-73090138 Msa0520530:CDS 35.0%
! ATTTTGTCACTACAGAGGAT+TGG + chr3_4:73090191-73090210 Msa0520530:three_prime_UTR 35.0%
CAAGATGCATTTGGACATTA+TGG + chr3_4:73090390-73090409 Msa0520530:three_prime_UTR 35.0%
!! ACAATTTCACCCCTTTTTCA+GGG - chr3_4:73090428-73090447 None:intergenic 35.0%
! AACAATTTCACCCCTTTTTC+AGG - chr3_4:73090429-73090448 None:intergenic 35.0%
TTCATCCTTTCCTTCCATCT+TGG + chr3_4:73087606-73087625 Msa0520530:CDS 40.0%
!!! TAGCTGTAGTTTTGGTCACT+TGG + chr3_4:73087829-73087848 Msa0520530:intron 40.0%
! TTTGGTCACTTGGTGGAAAA+TGG + chr3_4:73087839-73087858 Msa0520530:intron 40.0%
! ATGGTGGAGAAAGAAAGTGA+AGG + chr3_4:73087953-73087972 Msa0520530:intron 40.0%
GTTAGTCACAAATTGGCTGT+AGG + chr3_4:73088165-73088184 Msa0520530:intron 40.0%
GTTAAGGGATAAAGAGTTGG+AGG + chr3_4:73088226-73088245 Msa0520530:intron 40.0%
! CTGCTAGTTTGAAAAAGTGC+CGG - chr3_4:73088382-73088401 None:intergenic 40.0%
GCTATTATACATGGAACACG+TGG - chr3_4:73088457-73088476 None:intergenic 40.0%
! CATCTTCAAAGGTACATGGT+TGG - chr3_4:73088647-73088666 None:intergenic 40.0%
! GTCTCATCTTCAAAGGTACA+TGG - chr3_4:73088651-73088670 None:intergenic 40.0%
!! TGACACAGATAGAGAGTACT+AGG + chr3_4:73088781-73088800 Msa0520530:intron 40.0%
!! GATTTGGTGTTCGTTAAGAG+TGG + chr3_4:73088818-73088837 Msa0520530:intron 40.0%
CTTCTGCATCAGAGATGAAT+TGG + chr3_4:73088883-73088902 Msa0520530:intron 40.0%
GGTATGCCTTAATGTACTGT+AGG - chr3_4:73089667-73089686 None:intergenic 40.0%
ACAGTACATTAAGGCATACC+AGG + chr3_4:73089667-73089686 Msa0520530:CDS 40.0%
CAGTACATTAAGGCATACCA+GGG + chr3_4:73089668-73089687 Msa0520530:CDS 40.0%
TCAGCAAATTGAGATTCAGG+GGG - chr3_4:73089731-73089750 None:intergenic 40.0%
GCTTCAGCAAATTGAGATTC+AGG - chr3_4:73089734-73089753 None:intergenic 40.0%
ATGCCTTAACACCACAGTTT+GGG - chr3_4:73089763-73089782 None:intergenic 40.0%
TCAACTCTTGCTCGTATAAC+TGG + chr3_4:73089782-73089801 Msa0520530:CDS 40.0%
TCGTATAACTGGTCGTTTCT+TGG + chr3_4:73089793-73089812 Msa0520530:CDS 40.0%
CGTATAACTGGTCGTTTCTT+GGG + chr3_4:73089794-73089813 Msa0520530:CDS 40.0%
GTATAACTGGTCGTTTCTTG+GGG + chr3_4:73089795-73089814 Msa0520530:CDS 40.0%
AGCTCTTCTCGTTTCTTATC+AGG - chr3_4:73089865-73089884 None:intergenic 40.0%
!! AGCACATTTACTTTTGAGGG+TGG + chr3_4:73089995-73090014 Msa0520530:CDS 40.0%
!! ATTTACTTTTGAGGGTGGTG+TGG + chr3_4:73090000-73090019 Msa0520530:CDS 40.0%
GTGGCTAAAGAAACAAAACC+AGG + chr3_4:73090019-73090038 Msa0520530:CDS 40.0%
ACAAAACCAGGTTGCTATAG+TGG + chr3_4:73090031-73090050 Msa0520530:CDS 40.0%
CCTCTGTAGTGACAAAATGA+AGG - chr3_4:73090189-73090208 None:intergenic 40.0%
! CCTTCATTTTGTCACTACAG+AGG + chr3_4:73090186-73090205 Msa0520530:three_prime_UTR 40.0%
GTGTCTGCTTCAATGTAGTG+AGG - chr3_4:73087513-73087532 None:intergenic 45.0%
AAGCACCAAGATGGAAGGAA+AGG - chr3_4:73087614-73087633 None:intergenic 45.0%
!! TTCCATCTTGGTGCTTCTAC+CGG + chr3_4:73087618-73087637 Msa0520530:CDS 45.0%
GGACTTACTTTGAGTAGCAC+CGG - chr3_4:73087640-73087659 None:intergenic 45.0%
!!! CTGTAGTTTTGGTCACTTGG+TGG + chr3_4:73087832-73087851 Msa0520530:intron 45.0%
AGTCACAAATTGGCTGTAGG+AGG + chr3_4:73088168-73088187 Msa0520530:intron 45.0%
AAATTGGCTGTAGGAGGACA+GGG + chr3_4:73088174-73088193 Msa0520530:intron 45.0%
TGGAGGTCTGAGTTCAAACT+CGG + chr3_4:73088243-73088262 Msa0520530:intron 45.0%
!!! CGGCACTTTTTCAAACTAGC+AGG + chr3_4:73088380-73088399 Msa0520530:intron 45.0%
! CGGGTCGTTGCTATTATACA+TGG - chr3_4:73088466-73088485 None:intergenic 45.0%
AAAAGAGCAGCAACCAGCAA+CGG - chr3_4:73088971-73088990 None:intergenic 45.0%
TTACATGTGTGCTTCCCAGA+TGG + chr3_4:73089073-73089092 Msa0520530:intron 45.0%
GCATTCACTGTTACCCACTT+TGG + chr3_4:73089487-73089506 Msa0520530:intron 45.0%
! CAGCTACTAACTTGTCTGCA+GGG - chr3_4:73089574-73089593 None:intergenic 45.0%
CGAAAACAGAACTGCTCACA+AGG + chr3_4:73089601-73089620 Msa0520530:CDS 45.0%
TCTCGCCCTACAGTACATTA+AGG + chr3_4:73089658-73089677 Msa0520530:CDS 45.0%
TGCCTTAACACCACAGTTTG+GGG - chr3_4:73089762-73089781 None:intergenic 45.0%
GATGCCTTAACACCACAGTT+TGG - chr3_4:73089764-73089783 None:intergenic 45.0%
GTGCATGGACATACTTAGTC+TGG - chr3_4:73089823-73089842 None:intergenic 45.0%
TAAGTATGTCCATGCACCTG+AGG + chr3_4:73089826-73089845 Msa0520530:CDS 45.0%
AGGATTTCAGAAAACGCCTC+AGG - chr3_4:73089845-73089864 None:intergenic 45.0%
! ATGGAGGGTCTCCTTATTTC+TGG + chr3_4:73089942-73089961 Msa0520530:CDS 45.0%
TTAGCTCCACTATAGCAACC+TGG - chr3_4:73090040-73090059 None:intergenic 45.0%
AATAGCCACATCACACTGAC+TGG - chr3_4:73090094-73090113 None:intergenic 45.0%
ATGTACCCTAGCCCTGAAAA+AGG + chr3_4:73090414-73090433 Msa0520530:three_prime_UTR 45.0%
TGTACCCTAGCCCTGAAAAA+GGG + chr3_4:73090415-73090434 Msa0520530:three_prime_UTR 45.0%
!! GGTAGAAGCACCAAGATGGA+AGG - chr3_4:73087619-73087638 None:intergenic 50.0%
CAAATTGGCTGTAGGAGGAC+AGG + chr3_4:73088173-73088192 Msa0520530:intron 50.0%
GCTAATTATGTGGTCTCCCC+TGG + chr3_4:73088507-73088526 Msa0520530:intron 50.0%
GGCAGAAGAATCCACCATCT+GGG - chr3_4:73089090-73089109 None:intergenic 50.0%
! GCAGCTACTAACTTGTCTGC+AGG - chr3_4:73089575-73089594 None:intergenic 50.0%
AAGGCATACCAGGGTGATTG+TGG + chr3_4:73089677-73089696 Msa0520530:CDS 50.0%
TTGAGATTCAGGGGGCTTCT+TGG - chr3_4:73089723-73089742 None:intergenic 50.0%
! GAAGCTTTTGCCCCAAACTG+TGG + chr3_4:73089749-73089768 Msa0520530:CDS 50.0%
! TGCCCCAAACTGTGGTGTTA+AGG + chr3_4:73089757-73089776 Msa0520530:CDS 50.0%
!! CAAGTTGGTGCTGCTGTAAC+CGG + chr3_4:73089914-73089933 Msa0520530:CDS 50.0%
!! CTGTAACCGGTACTGATGGA+GGG + chr3_4:73089927-73089946 Msa0520530:CDS 50.0%
GGAGCTAATGATGTGTGCAG+TGG + chr3_4:73090052-73090071 Msa0520530:CDS 50.0%
TGTGCAGTGGTGCTCATGTT+TGG + chr3_4:73090065-73090084 Msa0520530:CDS 50.0%
GTACCCTAGCCCTGAAAAAG+GGG + chr3_4:73090416-73090435 Msa0520530:three_prime_UTR 50.0%
!!! TCACCCCTTTTTCAGGGCTA+GGG - chr3_4:73090422-73090441 None:intergenic 50.0%
!!! TTCACCCCTTTTTCAGGGCT+AGG - chr3_4:73090423-73090442 None:intergenic 50.0%
!! CACCGGTAGAAGCACCAAGA+TGG - chr3_4:73087623-73087642 None:intergenic 55.0%
GGAGGGTTGGGTAGTTCAAC+TGG + chr3_4:73088198-73088217 Msa0520530:intron 55.0%
CATGTGTGCTTCCCAGATGG+TGG + chr3_4:73089076-73089095 Msa0520530:intron 55.0%
CGGCAGAAGAATCCACCATC+TGG - chr3_4:73089091-73089110 None:intergenic 55.0%
!! CAGGGTGATTGTGGTGCTGT+GGG + chr3_4:73089686-73089705 Msa0520530:CDS 55.0%
CAGAAAACGCCTCAGGTGCA+TGG - chr3_4:73089838-73089857 None:intergenic 55.0%
! GCTGCTGTAACCGGTACTGA+TGG + chr3_4:73089923-73089942 Msa0520530:CDS 55.0%
!! GCTGTAACCGGTACTGATGG+AGG + chr3_4:73089926-73089945 Msa0520530:CDS 55.0%
AGGAGACCCTCCATCAGTAC+CGG - chr3_4:73089936-73089955 None:intergenic 55.0%
GGTCACCAGTCAGTGTGATG+TGG + chr3_4:73090086-73090105 Msa0520530:CDS 55.0%
TTGGCTGTAGGAGGACAGGG+CGG + chr3_4:73088177-73088196 Msa0520530:intron 60.0%
CCACAGCACCACAATCACCC+TGG - chr3_4:73089688-73089707 None:intergenic 60.0%
! CCAGGGTGATTGTGGTGCTG+TGG + chr3_4:73089685-73089704 Msa0520530:CDS 60.0%
!! GGTGATTGTGGTGCTGTGGG+TGG + chr3_4:73089689-73089708 Msa0520530:CDS 60.0%
CTGTAGGAGGACAGGGCGGA+GGG + chr3_4:73088181-73088200 Msa0520530:intron 65.0%
TAGGGCGAGACATGCGAGCC+CGG - chr3_4:73089648-73089667 None:intergenic 65.0%
GCTGTAGGAGGACAGGGCGG+AGG + chr3_4:73088180-73088199 Msa0520530:intron 70.0%
AGGAGGACAGGGCGGAGGGT+TGG + chr3_4:73088185-73088204 Msa0520530:intron 70.0%
GGAGGACAGGGCGGAGGGTT+GGG + chr3_4:73088186-73088205 Msa0520530:intron 70.0%
AGGGCGAGACATGCGAGCCC+GGG - chr3_4:73089647-73089666 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 73087492 73090508 73087492 ID=Msa0520530;Name=Msa0520530
chr3_4 mRNA 73087492 73090508 73087492 ID=Msa0520530-mRNA-1;Parent=Msa0520530;Name=Msa0520530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=81|1|1|1|1|1|2|349|227
chr3_4 exon 73087492 73087651 73087492 ID=Msa0520530-mRNA-1:exon:20370;Parent=Msa0520530-mRNA-1
chr3_4 exon 73089555 73090508 73089555 ID=Msa0520530-mRNA-1:exon:20371;Parent=Msa0520530-mRNA-1
chr3_4 five_prime_UTR 73087492 73087572 73087492 ID=Msa0520530-mRNA-1:five_prime_utr;Parent=Msa0520530-mRNA-1
chr3_4 CDS 73087573 73087651 73087573 ID=Msa0520530-mRNA-1:cds;Parent=Msa0520530-mRNA-1
chr3_4 CDS 73089555 73090159 73089555 ID=Msa0520530-mRNA-1:cds;Parent=Msa0520530-mRNA-1
chr3_4 three_prime_UTR 73090160 73090508 73090160 ID=Msa0520530-mRNA-1:three_prime_utr;Parent=Msa0520530-mRNA-1
Gene Sequence

>Msa0520530

ATGCCTAAAACTTTCACACTGTGTTCTCTTTTCTTCATCCTTTCCTTCCATCTTGGTGCTTCTACCGGTGCTACTCAAATCAAAGTTACTGATAACCCTGCAGACAAGTTAGTAGCTGCTATCAACGAAAACAGAACTGCTCACAAGGATTCATCTTTATTTGACAATCCCGGGCTCGCATGTCTCGCCCTACAGTACATTAAGGCATACCAGGGTGATTGTGGTGCTGTGGGTGGATCTGATGCCAAGAAGCCCCCTGAATCTCAATTTGCTGAAGCTTTTGCCCCAAACTGTGGTGTTAAGGCATCAACTCTTGCTCGTATAACTGGTCGTTTCTTGGGGTGCCAGACTAAGTATGTCCATGCACCTGAGGCGTTTTCTGAAATCCTGATAAGAAACGAGAAGAGCTTAGACATACTCTACAGTAAGAATCACACTCAAGTTGGTGCTGCTGTAACCGGTACTGATGGAGGGTCTCCTTATTTCTGGTGTGTGTTGTTTAGTAATGGCAAACCTAACAGCACATTTACTTTTGAGGGTGGTGTGGCTAAAGAAACAAAACCAGGTTGCTATAGTGGAGCTAATGATGTGTGCAGTGGTGCTCATGTTTGGTCACCAGTCAGTGTGATGTGGCTATTTGTTGCTTCAGTTTCAATTGCAATGGGGTTTGCTTTTCCATTGTGA
Protein sequence

>Msa0520530

MPKTFTLCSLFFILSFHLGASTGATQIKVTDNPADKLVAAINENRTAHKDSSLFDNPGLACLALQYIKAYQGDCGAVGGSDAKKPPESQFAEAFAPNCGVKASTLARITGRFLGCQTKYVHAPEAFSEILIRNEKSLDILYSKNHTQVGAAVTGTDGGSPYFWCVLFSNGKPNSTFTFEGGVAKETKPGCYSGANDVCSGAHVWSPVSVMWLFVASVSIAMGFAFPL*