AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0476540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0476540 MtrunA17_Chr3g0136301 96.070 229 7 1 46 272 1 229 2.91e-164 454
Msa0476540 MtrunA17_Chr8g0336911 45.026 191 103 2 77 266 30 219 1.28e-53 173
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0476540 AT1G02180.1 42.529 174 96 4 77 248 29 200 1.96e-42 145

Find 79 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAGGGTCTCCTTATTTC+TGG 0.118257 3_3:+80368063 Msa0476540:CDS
TTCATCCTTTCCTTCCTTCT+TGG 0.129768 3_3:+80365721 Msa0476540:CDS
CAAGATGCATTTGGACATTA+TGG 0.174203 3_3:+80368511 Msa0476540:three_prime_UTR
AAGTACCTTCTTAAAGATTC+TGG 0.222866 3_3:-80365516 None:intergenic
TCGTATAACTGGTCGTTTCT+TGG 0.250063 3_3:+80367914 Msa0476540:CDS
GCTTCAGCAAATTGAGATTC+AGG 0.261524 3_3:-80367852 None:intergenic
AGCTCTTCTCGTTTCTTATC+AGG 0.274033 3_3:-80367983 None:intergenic
CGTATAACTGGTCGTTTCTT+GGG 0.276791 3_3:+80367915 Msa0476540:CDS
ATGTACCCTAGCCCTGAAAA+AGG 0.292502 3_3:+80368535 Msa0476540:three_prime_UTR
ATGCCTTAACACCACAGTTT+GGG 0.323486 3_3:-80367881 None:intergenic
AACAACACACACCAGAAATA+AGG 0.333485 3_3:-80368074 None:intergenic
GATGCCTTAACACCACAGTT+TGG 0.357391 3_3:-80367882 None:intergenic
ATTGGAATTCAAGATGCATT+TGG 0.358573 3_3:+80368502 Msa0476540:three_prime_UTR
TCAACTCTTGCTCGTATAAC+TGG 0.364017 3_3:+80367903 Msa0476540:CDS
TCAAAAGTAAATGTGCTGTT+AGG 0.374585 3_3:-80368110 None:intergenic
TGCCCCAAACTGTGGTGTTA+AGG 0.376413 3_3:+80367878 Msa0476540:CDS
GCATATGAAACATGTGATAT+TGG 0.378061 3_3:+80368427 Msa0476540:three_prime_UTR
GCTTCAGTTTCAATTGCAAT+GGG 0.382192 3_3:+80368239 Msa0476540:CDS
CAAGTTGGTGCTGCTGTAAC+CGG 0.391700 3_3:+80368035 Msa0476540:CDS
TTCCTTCTTGGTGCTTCTAC+CGG 0.398930 3_3:+80365733 Msa0476540:CDS
ACAAAACCAGGTTGCTATAG+TGG 0.403030 3_3:+80368152 Msa0476540:CDS
TGCTTCAGTTTCAATTGCAA+TGG 0.413253 3_3:+80368238 Msa0476540:CDS
TGCTTTGATTGTTATACGAT+TGG 0.413715 3_3:+80368484 Msa0476540:three_prime_UTR
TCTCGCCCTACAGTACATTA+AGG 0.413855 3_3:+80367779 Msa0476540:CDS
CCACTCACTCACACGTTTGG+TGG 0.418107 3_3:+80365597 Msa0476540:CDS
ACCCCACTCACTCACACGTT+TGG 0.422230 3_3:+80365594 Msa0476540:CDS
TCATCTTTATTTGACAATCC+CGG 0.454811 3_3:+80367747 Msa0476540:CDS
TGTGCAGTGGTGCTCATGTT+TGG 0.457962 3_3:+80368186 Msa0476540:CDS
ATATCACATGTTTCATATGC+AGG 0.471229 3_3:-80368424 None:intergenic
TAGGGCGAGACATGCGAGCC+CGG 0.474850 3_3:-80367766 None:intergenic
GCAGCTACTAACTTGTCTGC+AGG 0.476484 3_3:-80367693 None:intergenic
CATCTTTATTTGACAATCCC+GGG 0.500468 3_3:+80367748 Msa0476540:CDS
TGGTGTGTGTTGTTTAGTAA+TGG 0.500489 3_3:+80368083 Msa0476540:CDS
GGTGATTGTGGTGCTGTGGG+TGG 0.502206 3_3:+80367810 Msa0476540:CDS
GGTATGCCTTAATGTACTGT+AGG 0.503759 3_3:-80367785 None:intergenic
CAGGGTGATTGTGGTGCTGT+GGG 0.510763 3_3:+80367807 Msa0476540:CDS
CTTCAGCAAATTGAGATTCA+GGG 0.511447 3_3:-80367851 None:intergenic
CGAAAACAGAACTGCTCACA+AGG 0.516931 3_3:+80367722 Msa0476540:CDS
GTGCATGGACATACTTAGTC+TGG 0.524594 3_3:-80367941 None:intergenic
GTATAACTGGTCGTTTCTTG+GGG 0.529253 3_3:+80367916 Msa0476540:CDS
CCAGGGTGATTGTGGTGCTG+TGG 0.539111 3_3:+80367806 Msa0476540:CDS
AATAGCCACATCACACTGAC+TGG 0.539928 3_3:-80368212 None:intergenic
TACTTCCAGAATCTTTAAGA+AGG 0.542813 3_3:+80365511 Msa0476540:five_prime_UTR
AAGGCATACCAGGGTGATTG+TGG 0.548506 3_3:+80367798 Msa0476540:CDS
CCACCAAACGTGTGAGTGAG+TGG 0.548652 3_3:-80365597 None:intergenic
ACAGTACATTAAGGCATACC+AGG 0.557681 3_3:+80367788 Msa0476540:CDS
CAGAAAACGCCTCAGGTGCA+TGG 0.559426 3_3:-80367956 None:intergenic
GCTGCTGTAACCGGTACTGA+TGG 0.569000 3_3:+80368044 Msa0476540:CDS
AGTAAGAATCACACTCAAGT+TGG 0.576187 3_3:+80368020 Msa0476540:CDS
AAGCACCAAGAAGGAAGGAA+AGG 0.578158 3_3:-80365726 None:intergenic
AGGGCGAGACATGCGAGCCC+GGG 0.579446 3_3:-80367765 None:intergenic
TGCCTTAACACCACAGTTTG+GGG 0.580489 3_3:-80367880 None:intergenic
CTTTGGTAGCATGTGAGTGG+TGG 0.580649 3_3:-80365542 None:intergenic
CACCGGTAGAAGCACCAAGA+AGG 0.580761 3_3:-80365735 None:intergenic
GCTGTAACCGGTACTGATGG+AGG 0.581331 3_3:+80368047 Msa0476540:CDS
AGGATTTCAGAAAACGCCTC+AGG 0.582162 3_3:-80367963 None:intergenic
CCTCTGTAGTGACAAAATGA+AGG 0.583962 3_3:-80368307 None:intergenic
GGTAGAAGCACCAAGAAGGA+AGG 0.585888 3_3:-80365731 None:intergenic
CACCAAACGTGTGAGTGAGT+GGG 0.588610 3_3:-80365596 None:intergenic
GTGAAAATTCATGATCACAA+TGG 0.597832 3_3:-80368272 None:intergenic
AGGAGACCCTCCATCAGTAC+CGG 0.597918 3_3:-80368054 None:intergenic
GTATGCCTTAATGTACTGTA+GGG 0.599616 3_3:-80367784 None:intergenic
TTTCTTTGGTAGCATGTGAG+TGG 0.604521 3_3:-80365545 None:intergenic
TTCAGCAAATTGAGATTCAG+GGG 0.606803 3_3:-80367850 None:intergenic
AGACTTACTTTGAGTAGCAC+CGG 0.607278 3_3:-80365752 None:intergenic
GGTCACCAGTCAGTGTGATG+TGG 0.613666 3_3:+80368207 Msa0476540:CDS
CAGCTACTAACTTGTCTGCA+GGG 0.614123 3_3:-80367692 None:intergenic
ACCAAACGTGTGAGTGAGTG+GGG 0.616568 3_3:-80365595 None:intergenic
CTTCAGTTTCAATTGCAATG+GGG 0.618866 3_3:+80368240 Msa0476540:CDS
CTGTAACCGGTACTGATGGA+GGG 0.623210 3_3:+80368048 Msa0476540:CDS
AAATAACAAAATCAAAACAA+AGG 0.645136 3_3:-80368362 None:intergenic
TTAGCTCCACTATAGCAACC+TGG 0.653166 3_3:-80368158 None:intergenic
TCAGCAAATTGAGATTCAGG+GGG 0.657704 3_3:-80367849 None:intergenic
CCACAGCACCACAATCACCC+TGG 0.672377 3_3:-80367806 None:intergenic
GTGGCTAAAGAAACAAAACC+AGG 0.685787 3_3:+80368140 Msa0476540:CDS
TAAGTATGTCCATGCACCTG+AGG 0.686474 3_3:+80367947 Msa0476540:CDS
GTGTCTGCTTCAATGTAGTG+AGG 0.687007 3_3:-80365625 None:intergenic
CAGTACATTAAGGCATACCA+GGG 0.710618 3_3:+80367789 Msa0476540:CDS
GGAGCTAATGATGTGTGCAG+TGG 0.715652 3_3:+80368173 Msa0476540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCATTGATTATATATA+TGG + chr3_3:80367446-80367465 Msa0476540:intron 10.0%
!!! TTTATTTTATATCTCAGATT+TGG + chr3_3:80365791-80365810 Msa0476540:intron 15.0%
!! TTATAACTCATTAAAGTTTA+TGG + chr3_3:80366157-80366176 Msa0476540:intron 15.0%
!! AGAAAATAAATATAACTATG+AGG - chr3_3:80367562-80367581 None:intergenic 15.0%
!! AAATAACAAAATCAAAACAA+AGG - chr3_3:80368365-80368384 None:intergenic 15.0%
!!! TGTTTTTTTCTTTCTTTCTT+TGG - chr3_3:80365562-80365581 None:intergenic 20.0%
!! AACAACTATTTACTAACATT+TGG + chr3_3:80366409-80366428 Msa0476540:intron 20.0%
!! CATTTGGCATTAAAAAAAAT+TGG + chr3_3:80366425-80366444 Msa0476540:intron 20.0%
!! CATTAAAAAAAATTGGTTGT+AGG + chr3_3:80366432-80366451 Msa0476540:intron 20.0%
!! GACAATATAAAATGAAATCA+AGG - chr3_3:80366527-80366546 None:intergenic 20.0%
!! AAAAAAATTGAACTGTTATG+TGG + chr3_3:80366708-80366727 Msa0476540:intron 20.0%
!! AAATATGTAACCTTAATCAA+AGG - chr3_3:80367422-80367441 None:intergenic 20.0%
!! ATAAATATAACTATGAGGTA+GGG - chr3_3:80367557-80367576 None:intergenic 20.0%
!! AATAAATATAACTATGAGGT+AGG - chr3_3:80367558-80367577 None:intergenic 20.0%
!!! ATGAATTTTCACTTGATTTA+GGG + chr3_3:80368283-80368302 Msa0476540:three_prime_UTR 20.0%
! TCTTGCAAGTTGTATTAAAA+TGG + chr3_3:80366001-80366020 Msa0476540:intron 25.0%
!! ATTTGGAACTTTCACTATAA+TGG + chr3_3:80366050-80366069 Msa0476540:intron 25.0%
! TATGTTAGTTAGTCACAAAT+TGG + chr3_3:80366275-80366294 Msa0476540:intron 25.0%
! TTAAACAATAAGCATCAAAC+GGG - chr3_3:80366606-80366625 None:intergenic 25.0%
! CTTAAACAATAAGCATCAAA+CGG - chr3_3:80366607-80366626 None:intergenic 25.0%
! TTGTTTAAGAGCTAATTATG+TGG + chr3_3:80366618-80366637 Msa0476540:intron 25.0%
! TTTCAACAAAATTAATCCAG+GGG - chr3_3:80366647-80366666 None:intergenic 25.0%
! CTTTCAACAAAATTAATCCA+GGG - chr3_3:80366648-80366667 None:intergenic 25.0%
! TCTTTCAACAAAATTAATCC+AGG - chr3_3:80366649-80366668 None:intergenic 25.0%
!! ATGACTTTTGTAGTGAAATT+AGG + chr3_3:80366838-80366857 Msa0476540:intron 25.0%
! TGAAATTAGGACTAAACTTA+TGG + chr3_3:80366851-80366870 Msa0476540:intron 25.0%
!!! TGAATATCTAGCTTTCATTA+TGG + chr3_3:80366981-80367000 Msa0476540:intron 25.0%
! AGACAAACTATAACAAATGT+TGG - chr3_3:80367166-80367185 None:intergenic 25.0%
!!! ACATTTGTTATAGTTTGTCT+AGG + chr3_3:80367166-80367185 Msa0476540:intron 25.0%
! TAATCAAAGGATAAAAGGAA+AGG - chr3_3:80367409-80367428 None:intergenic 25.0%
!! TTCCTTTTATCCTTTGATTA+AGG + chr3_3:80367409-80367428 Msa0476540:intron 25.0%
! AACCTTAATCAAAGGATAAA+AGG - chr3_3:80367414-80367433 None:intergenic 25.0%
!! CATGAATTTTCACTTGATTT+AGG + chr3_3:80368282-80368301 Msa0476540:three_prime_UTR 25.0%
!! TGAATTTTCACTTGATTTAG+GGG + chr3_3:80368284-80368303 Msa0476540:three_prime_UTR 25.0%
!!! TGATTTTGTTATTTGTTGAG+AGG + chr3_3:80368371-80368390 Msa0476540:three_prime_UTR 25.0%
!! AAATTGTTGCATAAGCTTTT+TGG + chr3_3:80368562-80368581 Msa0476540:three_prime_UTR 25.0%
! TACTTCCAGAATCTTTAAGA+AGG + chr3_3:80365511-80365530 Msa0476540:five_prime_UTR 30.0%
AAGTACCTTCTTAAAGATTC+TGG - chr3_3:80365519-80365538 None:intergenic 30.0%
ATTACTGTGAAATGCTACAT+TGG - chr3_3:80365920-80365939 None:intergenic 30.0%
TGAAAGTTCCAAATCAGATA+CGG - chr3_3:80366044-80366063 None:intergenic 30.0%
AGGAAAAAGAGAACAAGTTA+AGG + chr3_3:80366089-80366108 Msa0476540:intron 30.0%
ATTGTCCAAATCAAGAATCA+CGG - chr3_3:80366120-80366139 None:intergenic 30.0%
GAGTTATAAAGACGAAGAAA+TGG - chr3_3:80366146-80366165 None:intergenic 30.0%
!!! TTATGGTCTTTGTTTTAGTG+TGG + chr3_3:80366174-80366193 Msa0476540:intron 30.0%
TTGTGACTAACTAACATAAG+TGG - chr3_3:80366273-80366292 None:intergenic 30.0%
TTTATAACTGTATACGCTTC+CGG + chr3_3:80366481-80366500 Msa0476540:intron 30.0%
! CATTTTATATTGTCTCTGCT+CGG + chr3_3:80366533-80366552 Msa0476540:intron 30.0%
AATTGAACTGTTATGTGGTT+AGG + chr3_3:80366713-80366732 Msa0476540:intron 30.0%
ACAAAAGTCATAACTTTGCA+TGG - chr3_3:80366829-80366848 None:intergenic 30.0%
AAACTTATGGAAGCCTTAAA+TGG + chr3_3:80366864-80366883 Msa0476540:intron 30.0%
! GGATTTAGGAATTTGAGATT+TGG + chr3_3:80366923-80366942 Msa0476540:intron 30.0%
ACAGAGTTTAGTGTATACAA+AGG + chr3_3:80367042-80367061 Msa0476540:intron 30.0%
CAGAGTTTAGTGTATACAAA+GGG + chr3_3:80367043-80367062 Msa0476540:intron 30.0%
AAGATAAATAAAACCGTTGC+TGG + chr3_3:80367076-80367095 Msa0476540:intron 30.0%
ACAATGATAGAAGCAATATC+AGG - chr3_3:80367129-80367148 None:intergenic 30.0%
AATTTAGAGAACACCAAAGT+GGG - chr3_3:80367624-80367643 None:intergenic 30.0%
TAATTTAGAGAACACCAAAG+TGG - chr3_3:80367625-80367644 None:intergenic 30.0%
! TCATCTTTATTTGACAATCC+CGG + chr3_3:80367747-80367766 Msa0476540:CDS 30.0%
! TCAAAAGTAAATGTGCTGTT+AGG - chr3_3:80368113-80368132 None:intergenic 30.0%
!! TAACAGCACATTTACTTTTG+AGG + chr3_3:80368112-80368131 Msa0476540:CDS 30.0%
!! AACAGCACATTTACTTTTGA+GGG + chr3_3:80368113-80368132 Msa0476540:CDS 30.0%
GTGAAAATTCATGATCACAA+TGG - chr3_3:80368275-80368294 None:intergenic 30.0%
ATATCACATGTTTCATATGC+AGG - chr3_3:80368427-80368446 None:intergenic 30.0%
GCATATGAAACATGTGATAT+TGG + chr3_3:80368427-80368446 Msa0476540:three_prime_UTR 30.0%
! CTCTTGTAATTTTCTCATCT+AGG + chr3_3:80368450-80368469 Msa0476540:three_prime_UTR 30.0%
TGCTTTGATTGTTATACGAT+TGG + chr3_3:80368484-80368503 Msa0476540:three_prime_UTR 30.0%
ATTGGAATTCAAGATGCATT+TGG + chr3_3:80368502-80368521 Msa0476540:three_prime_UTR 30.0%
TATGTAGCATGAGTTTGATC+TGG + chr3_3:80365832-80365851 Msa0476540:intron 35.0%
! AATTCTGCTAGCTGTAGTTT+TGG + chr3_3:80365937-80365956 Msa0476540:intron 35.0%
TGGAACTTTCACTATAATGG+TGG + chr3_3:80366053-80366072 Msa0476540:intron 35.0%
ATATCCCGTGATTCTTGATT+TGG + chr3_3:80366112-80366131 Msa0476540:intron 35.0%
TTGTCCAAATCAAGAATCAC+GGG - chr3_3:80366119-80366138 None:intergenic 35.0%
!! GTTTTAGTGTGGAGTTTCAT+TGG + chr3_3:80366185-80366204 Msa0476540:intron 35.0%
!! AGTTCAACTGGTTTGAGTTA+AGG + chr3_3:80366330-80366349 Msa0476540:intron 35.0%
!! GTTCAACTGGTTTGAGTTAA+GGG + chr3_3:80366331-80366350 Msa0476540:intron 35.0%
TGAGTTAAGGGATAAAGAGT+TGG + chr3_3:80366343-80366362 Msa0476540:intron 35.0%
! ACGTGGACATAGTTCATTTT+TGG - chr3_3:80366561-80366580 None:intergenic 35.0%
CTAGAGCTAGAAACATAGAT+AGG - chr3_3:80366687-80366706 None:intergenic 35.0%
!! CTATCTATGTTTCTAGCTCT+AGG + chr3_3:80366685-80366704 Msa0476540:intron 35.0%
AACAGAAGTCTCATCTTCAA+AGG - chr3_3:80366779-80366798 None:intergenic 35.0%
AAAGTCATAACTTTGCATGG+TGG - chr3_3:80366826-80366845 None:intergenic 35.0%
TACAAAGAGGTCACCATTTA+AGG - chr3_3:80366880-80366899 None:intergenic 35.0%
TATCTGTGTCAAGTACAAAG+AGG - chr3_3:80366893-80366912 None:intergenic 35.0%
!! GATAGAGAGTACTAGGATTT+AGG + chr3_3:80366909-80366928 Msa0476540:intron 35.0%
TAAGAGTGGAGAAAATGTTG+TGG + chr3_3:80366953-80366972 Msa0476540:intron 35.0%
TATACACTAAACTCTGTCTC+TGG - chr3_3:80367039-80367058 None:intergenic 35.0%
ATGATAGAAGCAATATCAGG+AGG - chr3_3:80367126-80367145 None:intergenic 35.0%
TGAGTTTCTCAACAGATTCT+CGG - chr3_3:80367232-80367251 None:intergenic 35.0%
AAAGTAGGCTGTTTATTGTG+AGG - chr3_3:80367479-80367498 None:intergenic 35.0%
! CACAATAAACAGCCTACTTT+TGG + chr3_3:80367479-80367498 Msa0476540:intron 35.0%
!! TTTTTCAGTGAGCCAAAAGT+AGG - chr3_3:80367494-80367513 None:intergenic 35.0%
! CATCTTTATTTGACAATCCC+GGG + chr3_3:80367748-80367767 Msa0476540:CDS 35.0%
GTATGCCTTAATGTACTGTA+GGG - chr3_3:80367787-80367806 None:intergenic 35.0%
TTCAGCAAATTGAGATTCAG+GGG - chr3_3:80367853-80367872 None:intergenic 35.0%
CTTCAGCAAATTGAGATTCA+GGG - chr3_3:80367854-80367873 None:intergenic 35.0%
AGTAAGAATCACACTCAAGT+TGG + chr3_3:80368020-80368039 Msa0476540:CDS 35.0%
AACAACACACACCAGAAATA+AGG - chr3_3:80368077-80368096 None:intergenic 35.0%
!! TGGTGTGTGTTGTTTAGTAA+TGG + chr3_3:80368083-80368102 Msa0476540:CDS 35.0%
TGCTTCAGTTTCAATTGCAA+TGG + chr3_3:80368238-80368257 Msa0476540:CDS 35.0%
GCTTCAGTTTCAATTGCAAT+GGG + chr3_3:80368239-80368258 Msa0476540:CDS 35.0%
CTTCAGTTTCAATTGCAATG+GGG + chr3_3:80368240-80368259 Msa0476540:CDS 35.0%
! ATTTTGTCACTACAGAGGAT+TGG + chr3_3:80368312-80368331 Msa0476540:three_prime_UTR 35.0%
CAAGATGCATTTGGACATTA+TGG + chr3_3:80368511-80368530 Msa0476540:three_prime_UTR 35.0%
!! ACAATTTCACCCCTTTTTCA+GGG - chr3_3:80368549-80368568 None:intergenic 35.0%
! AACAATTTCACCCCTTTTTC+AGG - chr3_3:80368550-80368569 None:intergenic 35.0%
! TTTCTTTGGTAGCATGTGAG+TGG - chr3_3:80365548-80365567 None:intergenic 40.0%
! CAGCACAGTGTGAAAGTTTT+AGG - chr3_3:80365694-80365713 None:intergenic 40.0%
TTCATCCTTTCCTTCCTTCT+TGG + chr3_3:80365721-80365740 Msa0476540:CDS 40.0%
AGACTTACTTTGAGTAGCAC+CGG - chr3_3:80365755-80365774 None:intergenic 40.0%
!!! TAGCTGTAGTTTTGGTCACT+TGG + chr3_3:80365945-80365964 Msa0476540:intron 40.0%
! TTTGGTCACTTGGTGGAAAA+TGG + chr3_3:80365955-80365974 Msa0476540:intron 40.0%
AGAACTGTCCGTATCTGATT+TGG + chr3_3:80366033-80366052 Msa0476540:intron 40.0%
! ATGGTGGAGAAAGAAAGTGA+AGG + chr3_3:80366069-80366088 Msa0476540:intron 40.0%
AGTCACAAATTGGCTGTACT+AGG + chr3_3:80366285-80366304 Msa0476540:intron 40.0%
GTTAAGGGATAAAGAGTTGG+AGG + chr3_3:80366346-80366365 Msa0476540:intron 40.0%
! CTGCTAGTTTGAAAAAGTGC+CGG - chr3_3:80366503-80366522 None:intergenic 40.0%
GCTATTATACATGGAACACG+TGG - chr3_3:80366578-80366597 None:intergenic 40.0%
! CATCTTCAAAGGTACATGGT+TGG - chr3_3:80366768-80366787 None:intergenic 40.0%
! GTCTCATCTTCAAAGGTACA+TGG - chr3_3:80366772-80366791 None:intergenic 40.0%
!! TGACACAGATAGAGAGTACT+AGG + chr3_3:80366902-80366921 Msa0476540:intron 40.0%
!! GATTTGGTGTTCGTTAAGAG+TGG + chr3_3:80366939-80366958 Msa0476540:intron 40.0%
CTTCTGCATCAGAGATGAAT+TGG + chr3_3:80367004-80367023 Msa0476540:intron 40.0%
GGTATGCCTTAATGTACTGT+AGG - chr3_3:80367788-80367807 None:intergenic 40.0%
ACAGTACATTAAGGCATACC+AGG + chr3_3:80367788-80367807 Msa0476540:CDS 40.0%
CAGTACATTAAGGCATACCA+GGG + chr3_3:80367789-80367808 Msa0476540:CDS 40.0%
TCAGCAAATTGAGATTCAGG+GGG - chr3_3:80367852-80367871 None:intergenic 40.0%
GCTTCAGCAAATTGAGATTC+AGG - chr3_3:80367855-80367874 None:intergenic 40.0%
ATGCCTTAACACCACAGTTT+GGG - chr3_3:80367884-80367903 None:intergenic 40.0%
TCAACTCTTGCTCGTATAAC+TGG + chr3_3:80367903-80367922 Msa0476540:CDS 40.0%
TCGTATAACTGGTCGTTTCT+TGG + chr3_3:80367914-80367933 Msa0476540:CDS 40.0%
CGTATAACTGGTCGTTTCTT+GGG + chr3_3:80367915-80367934 Msa0476540:CDS 40.0%
GTATAACTGGTCGTTTCTTG+GGG + chr3_3:80367916-80367935 Msa0476540:CDS 40.0%
AGCTCTTCTCGTTTCTTATC+AGG - chr3_3:80367986-80368005 None:intergenic 40.0%
!! AGCACATTTACTTTTGAGGG+TGG + chr3_3:80368116-80368135 Msa0476540:CDS 40.0%
!! ATTTACTTTTGAGGGTGGTG+TGG + chr3_3:80368121-80368140 Msa0476540:CDS 40.0%
GTGGCTAAAGAAACAAAACC+AGG + chr3_3:80368140-80368159 Msa0476540:CDS 40.0%
ACAAAACCAGGTTGCTATAG+TGG + chr3_3:80368152-80368171 Msa0476540:CDS 40.0%
CCTCTGTAGTGACAAAATGA+AGG - chr3_3:80368310-80368329 None:intergenic 40.0%
! CCTTCATTTTGTCACTACAG+AGG + chr3_3:80368307-80368326 Msa0476540:three_prime_UTR 40.0%
GTGTCTGCTTCAATGTAGTG+AGG - chr3_3:80365628-80365647 None:intergenic 45.0%
AAGCACCAAGAAGGAAGGAA+AGG - chr3_3:80365729-80365748 None:intergenic 45.0%
!! TTCCTTCTTGGTGCTTCTAC+CGG + chr3_3:80365733-80365752 Msa0476540:CDS 45.0%
!!! CTGTAGTTTTGGTCACTTGG+TGG + chr3_3:80365948-80365967 Msa0476540:intron 45.0%
CACAAATTGGCTGTACTAGG+AGG + chr3_3:80366288-80366307 Msa0476540:intron 45.0%
ATTCGTCCGAGTTTGAACTC+AGG - chr3_3:80366373-80366392 None:intergenic 45.0%
!!! CGGCACTTTTTCAAACTAGC+AGG + chr3_3:80366501-80366520 Msa0476540:intron 45.0%
! CGGGTCGTTGCTATTATACA+TGG - chr3_3:80366587-80366606 None:intergenic 45.0%
AAAAGAGCAGCAACCAGCAA+CGG - chr3_3:80367092-80367111 None:intergenic 45.0%
TTACATGTGTGCTTCCCAGA+TGG + chr3_3:80367194-80367213 Msa0476540:intron 45.0%
GCATTCACTGTTACCCACTT+TGG + chr3_3:80367608-80367627 Msa0476540:intron 45.0%
! CAGCTACTAACTTGTCTGCA+GGG - chr3_3:80367695-80367714 None:intergenic 45.0%
CGAAAACAGAACTGCTCACA+AGG + chr3_3:80367722-80367741 Msa0476540:CDS 45.0%
TCTCGCCCTACAGTACATTA+AGG + chr3_3:80367779-80367798 Msa0476540:CDS 45.0%
TGCCTTAACACCACAGTTTG+GGG - chr3_3:80367883-80367902 None:intergenic 45.0%
GATGCCTTAACACCACAGTT+TGG - chr3_3:80367885-80367904 None:intergenic 45.0%
GTGCATGGACATACTTAGTC+TGG - chr3_3:80367944-80367963 None:intergenic 45.0%
TAAGTATGTCCATGCACCTG+AGG + chr3_3:80367947-80367966 Msa0476540:CDS 45.0%
AGGATTTCAGAAAACGCCTC+AGG - chr3_3:80367966-80367985 None:intergenic 45.0%
! ATGGAGGGTCTCCTTATTTC+TGG + chr3_3:80368063-80368082 Msa0476540:CDS 45.0%
TTAGCTCCACTATAGCAACC+TGG - chr3_3:80368161-80368180 None:intergenic 45.0%
AATAGCCACATCACACTGAC+TGG - chr3_3:80368215-80368234 None:intergenic 45.0%
ATGTACCCTAGCCCTGAAAA+AGG + chr3_3:80368535-80368554 Msa0476540:three_prime_UTR 45.0%
TGTACCCTAGCCCTGAAAAA+GGG + chr3_3:80368536-80368555 Msa0476540:three_prime_UTR 45.0%
! CTTTGGTAGCATGTGAGTGG+TGG - chr3_3:80365545-80365564 None:intergenic 50.0%
ACCAAACGTGTGAGTGAGTG+GGG - chr3_3:80365598-80365617 None:intergenic 50.0%
CACCAAACGTGTGAGTGAGT+GGG - chr3_3:80365599-80365618 None:intergenic 50.0%
!! GGTAGAAGCACCAAGAAGGA+AGG - chr3_3:80365734-80365753 None:intergenic 50.0%
ATTGGCTGTACTAGGAGGAC+AGG + chr3_3:80366293-80366312 Msa0476540:intron 50.0%
TTGGCTGTACTAGGAGGACA+GGG + chr3_3:80366294-80366313 Msa0476540:intron 50.0%
GGAGGTCCTGAGTTCAAACT+CGG + chr3_3:80366364-80366383 Msa0476540:intron 50.0%
GCTAATTATGTGGTCTCCCC+TGG + chr3_3:80366628-80366647 Msa0476540:intron 50.0%
GGCAGAAGAATCCACCATCT+GGG - chr3_3:80367211-80367230 None:intergenic 50.0%
! GCAGCTACTAACTTGTCTGC+AGG - chr3_3:80367696-80367715 None:intergenic 50.0%
AAGGCATACCAGGGTGATTG+TGG + chr3_3:80367798-80367817 Msa0476540:CDS 50.0%
TTGAGATTCAGGGGGCTTCT+TGG - chr3_3:80367844-80367863 None:intergenic 50.0%
! GAAGCTTTTGCCCCAAACTG+TGG + chr3_3:80367870-80367889 Msa0476540:CDS 50.0%
! TGCCCCAAACTGTGGTGTTA+AGG + chr3_3:80367878-80367897 Msa0476540:CDS 50.0%
!! CAAGTTGGTGCTGCTGTAAC+CGG + chr3_3:80368035-80368054 Msa0476540:CDS 50.0%
!! CTGTAACCGGTACTGATGGA+GGG + chr3_3:80368048-80368067 Msa0476540:CDS 50.0%
GGAGCTAATGATGTGTGCAG+TGG + chr3_3:80368173-80368192 Msa0476540:CDS 50.0%
TGTGCAGTGGTGCTCATGTT+TGG + chr3_3:80368186-80368205 Msa0476540:CDS 50.0%
GTACCCTAGCCCTGAAAAAG+GGG + chr3_3:80368537-80368556 Msa0476540:three_prime_UTR 50.0%
!!! TCACCCCTTTTTCAGGGCTA+GGG - chr3_3:80368543-80368562 None:intergenic 50.0%
!!! TTCACCCCTTTTTCAGGGCT+AGG - chr3_3:80368544-80368563 None:intergenic 50.0%
ACCCCACTCACTCACACGTT+TGG + chr3_3:80365594-80365613 Msa0476540:CDS 55.0%
CCACCAAACGTGTGAGTGAG+TGG - chr3_3:80365600-80365619 None:intergenic 55.0%
CCACTCACTCACACGTTTGG+TGG + chr3_3:80365597-80365616 Msa0476540:CDS 55.0%
!! CACCGGTAGAAGCACCAAGA+AGG - chr3_3:80365738-80365757 None:intergenic 55.0%
GGAGGGTTGGGTAGTTCAAC+TGG + chr3_3:80366318-80366337 Msa0476540:intron 55.0%
CATGTGTGCTTCCCAGATGG+TGG + chr3_3:80367197-80367216 Msa0476540:intron 55.0%
CGGCAGAAGAATCCACCATC+TGG - chr3_3:80367212-80367231 None:intergenic 55.0%
!! CAGGGTGATTGTGGTGCTGT+GGG + chr3_3:80367807-80367826 Msa0476540:CDS 55.0%
CAGAAAACGCCTCAGGTGCA+TGG - chr3_3:80367959-80367978 None:intergenic 55.0%
! GCTGCTGTAACCGGTACTGA+TGG + chr3_3:80368044-80368063 Msa0476540:CDS 55.0%
!! GCTGTAACCGGTACTGATGG+AGG + chr3_3:80368047-80368066 Msa0476540:CDS 55.0%
AGGAGACCCTCCATCAGTAC+CGG - chr3_3:80368057-80368076 None:intergenic 55.0%
GGTCACCAGTCAGTGTGATG+TGG + chr3_3:80368207-80368226 Msa0476540:CDS 55.0%
GCTGTACTAGGAGGACAGGG+CGG + chr3_3:80366297-80366316 Msa0476540:intron 60.0%
TACTAGGAGGACAGGGCGGA+GGG + chr3_3:80366301-80366320 Msa0476540:intron 60.0%
CCACAGCACCACAATCACCC+TGG - chr3_3:80367809-80367828 None:intergenic 60.0%
! CCAGGGTGATTGTGGTGCTG+TGG + chr3_3:80367806-80367825 Msa0476540:CDS 60.0%
!! GGTGATTGTGGTGCTGTGGG+TGG + chr3_3:80367810-80367829 Msa0476540:CDS 60.0%
GTACTAGGAGGACAGGGCGG+AGG + chr3_3:80366300-80366319 Msa0476540:intron 65.0%
TAGGGCGAGACATGCGAGCC+CGG - chr3_3:80367769-80367788 None:intergenic 65.0%
AGGAGGACAGGGCGGAGGGT+TGG + chr3_3:80366305-80366324 Msa0476540:intron 70.0%
GGAGGACAGGGCGGAGGGTT+GGG + chr3_3:80366306-80366325 Msa0476540:intron 70.0%
AGGGCGAGACATGCGAGCCC+GGG - chr3_3:80367768-80367787 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 80365504 80368629 80365504 ID=Msa0476540;Name=Msa0476540
chr3_3 mRNA 80365504 80368629 80365504 ID=Msa0476540-mRNA-1;Parent=Msa0476540;Name=Msa0476540-mRNA-1;_AED=0.00;_eAED=0.00;_QI=49|1|1|1|1|1|2|349|272
chr3_3 exon 80365504 80365766 80365504 ID=Msa0476540-mRNA-1:exon:21935;Parent=Msa0476540-mRNA-1
chr3_3 exon 80367676 80368629 80367676 ID=Msa0476540-mRNA-1:exon:21936;Parent=Msa0476540-mRNA-1
chr3_3 five_prime_UTR 80365504 80365552 80365504 ID=Msa0476540-mRNA-1:five_prime_utr;Parent=Msa0476540-mRNA-1
chr3_3 CDS 80365553 80365766 80365553 ID=Msa0476540-mRNA-1:cds;Parent=Msa0476540-mRNA-1
chr3_3 CDS 80367676 80368280 80367676 ID=Msa0476540-mRNA-1:cds;Parent=Msa0476540-mRNA-1
chr3_3 three_prime_UTR 80368281 80368629 80368281 ID=Msa0476540-mRNA-1:three_prime_utr;Parent=Msa0476540-mRNA-1
Gene Sequence

>Msa0476540

ATGCTACCAAAGAAAGAAAGAAAAAAACACAAGTTTGTTAGACCCCACTCACTCACACGTTTGGTGGATTTTCCTCACTACATTGAAGCAGACACAGATCTGTCTTCAATTTCTGTGTGTTCTGAAACAGTAACAATGCCTAAAACTTTCACACTGTGCTGTCTTTTCTTCATCCTTTCCTTCCTTCTTGGTGCTTCTACCGGTGCTACTCAAATCAAAGTTACTGATAACCCTGCAGACAAGTTAGTAGCTGCTATCAACGAAAACAGAACTGCTCACAAGGATTCATCTTTATTTGACAATCCCGGGCTCGCATGTCTCGCCCTACAGTACATTAAGGCATACCAGGGTGATTGTGGTGCTGTGGGTGGATCTGATGCCAAGAAGCCCCCTGAATCTCAATTTGCTGAAGCTTTTGCCCCAAACTGTGGTGTTAAGGCATCAACTCTTGCTCGTATAACTGGTCGTTTCTTGGGGTGCCAGACTAAGTATGTCCATGCACCTGAGGCGTTTTCTGAAATCCTGATAAGAAACGAGAAGAGCTTAGACATACTCTACAGTAAGAATCACACTCAAGTTGGTGCTGCTGTAACCGGTACTGATGGAGGGTCTCCTTATTTCTGGTGTGTGTTGTTTAGTAATGGCAAACCTAACAGCACATTTACTTTTGAGGGTGGTGTGGCTAAAGAAACAAAACCAGGTTGCTATAGTGGAGCTAATGATGTGTGCAGTGGTGCTCATGTTTGGTCACCAGTCAGTGTGATGTGGCTATTTGTTGCTTCAGTTTCAATTGCAATGGGGTTTGCTTTTCCATTGTGA
Protein sequence

>Msa0476540

MLPKKERKKHKFVRPHSLTRLVDFPHYIEADTDLSSISVCSETVTMPKTFTLCCLFFILSFLLGASTGATQIKVTDNPADKLVAAINENRTAHKDSSLFDNPGLACLALQYIKAYQGDCGAVGGSDAKKPPESQFAEAFAPNCGVKASTLARITGRFLGCQTKYVHAPEAFSEILIRNEKSLDILYSKNHTQVGAAVTGTDGGSPYFWCVLFSNGKPNSTFTFEGGVAKETKPGCYSGANDVCSGAHVWSPVSVMWLFVASVSIAMGFAFPL*