AlfalfaGEDB | Msa0521290

M. sativa cultivar zhongmu-4 / Msa0521290

KEGG: K00759
GO: GO:0003674 GO:0003824 GO:0003999 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005829 GO:0005886 GO:0006139 GO:0006144 GO:0006168 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0008150 GO:0008152 GO:0008509 GO:0009058 GO:0009112 GO:0009113 GO:0009987 GO:0012505 GO:0015075 GO:0015103 GO:0015114 GO:0015318 GO:0015698 GO:0016020 GO:0016740 GO:0016757 GO:0016763 GO:0018130 GO:0019438 GO:0022857 GO:0034220 GO:0034641 GO:0034654 GO:0035435 GO:0042440 GO:0043094 GO:0043096 GO:0043101 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046083 GO:0046084 GO:0046112 GO:0046148 GO:0046483 GO:0051179 GO:0051234 GO:0055085 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0098656 GO:0098660 GO:0098661 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00156.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0521290	XP_003603366.2	94.527	201	1	1	1	201	1	191	3.55e-132	379

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0521290	sp\|Q9LFP0\|APT5_ARATH	73.367	199	43	1	1	199	1	189	1.27e-99	289

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0521290	G7JAH8	94.527	201	1	1	1	201	1	191	1.70e-132	379

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0093850	Msa0521290	0.811172	7.995604e-51	-8.615850e-47
Msa0138360	Msa0521290	0.803585	3.249470e-49	-8.615850e-47
Msa0341690	Msa0521290	0.806671	7.345920e-50	-8.615850e-47
Msa0476920	Msa0521290	0.889325	2.679344e-73	-8.615850e-47
Msa0518030	Msa0521290	0.820585	6.345234e-53	-8.615850e-47
Msa0521290	Msa0713970	0.801819	7.519790e-49	-8.615850e-47
Msa0521290	Msa0829240	0.962515	5.720119e-121	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0521290	MtrunA17_Chr3g0137551	94.527	201	1	1	1	201	1	191	3.27e-136	379
Msa0521290	MtrunA17_Chr3g0134721	71.585	183	42	1	11	193	70	242	3.53e-91	267
Msa0521290	MtrunA17_Chr5g0399031	70.492	183	44	1	11	193	10	182	4.27e-87	254
Msa0521290	MtrunA17_Chr4g0056471	66.667	183	51	1	11	193	8	180	9.44e-82	241

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0521290	AT5G11160.1	73.367	199	43	1	1	199	1	189	1.30e-100	289
Msa0521290	AT1G80050.1	71.357	199	47	1	1	199	1	189	2.58e-98	283
Msa0521290	AT4G12440.3	68.586	191	49	2	5	195	3	182	6.04e-89	259
Msa0521290	AT4G12440.2	68.586	191	49	2	5	195	3	182	6.04e-89	259
Msa0521290	AT4G22570.1	67.368	190	52	1	6	195	4	183	2.49e-86	253
Msa0521290	AT1G27450.2	66.120	183	52	1	11	193	8	180	2.10e-84	248
Msa0521290	AT1G27450.4	66.120	183	52	1	11	193	8	180	2.10e-84	248
Msa0521290	AT1G27450.1	66.120	183	52	1	11	193	68	240	1.54e-83	248
Msa0521290	AT5G11160.2	77.703	148	33	0	1	148	1	148	9.60e-81	238
Msa0521290	AT1G80050.2	70.659	167	39	1	33	199	1	157	3.06e-78	231

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Find 60 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCACTTTATGTGCTTG+TGG	0.574929	3_4:-73950639	Msa0521290:CDS
AACTGCAATGCAATGGCAAT+GGG	0.513149	3_4:+73953933	None:intergenic
AAGCATTCATCTACTTGACG+TGG	0.677392	3_4:+73950614	None:intergenic
ACAGGTGGAACTTTGTCGGC+AGG	0.532728	3_4:-73951585	Msa0521290:CDS
ACGCAAACCTCGCAAGCTAC+CGG	0.479212	3_4:-73953127	Msa0521290:CDS
ACGTGCTAAGGCTGAAGTAG+TGG	0.569286	3_4:-73951422	Msa0521290:CDS
AGAATAAATATAATCAGTTT+TGG	0.254134	3_4:-73953857	Msa0521290:five_prime_UTR
AGAATGTTTGCCGAAGAAAA+TGG	0.424560	3_4:-73953793	Msa0521290:CDS
AGCTTGCGAGGTTTGCGTAA+AGG	0.464262	3_4:+73953132	None:intergenic
AGGGACGACTCTGATGGACT+GGG	0.649098	3_4:+73953738	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATTTATTCTGTGAAGTGTG+AGG	+	chr3_4:73950771-73950790	None:intergenic	30.0%
AGAAAAAAAGGAAGGAAAGA+AGG	+	chr3_4:73950955-73950974	None:intergenic	30.0%
ACTAAGTAATAAAATGGGTC+TGG	-	chr3_4:73951087-73951106	Msa0521290:intron	30.0%
CTAAGTAATAAAATGGGTCT+GGG	-	chr3_4:73951088-73951107	Msa0521290:intron	30.0%
ACAAAAAAAGTAGTGCATGA+GGG	+	chr3_4:73951305-73951324	None:intergenic	30.0%
AACAAAAAAAGTAGTGCATG+AGG	+	chr3_4:73951306-73951325	None:intergenic	30.0%
TTTCTTATATTTAGTACGGG+AGG	+	chr3_4:73951688-73951707	None:intergenic	30.0%
TATATTTATTTAGGAGGGGA+TGG	-	chr3_4:73951791-73951810	Msa0521290:intron	30.0%
TTCCATCACATAAAGTCAAT+TGG	-	chr3_4:73952085-73952104	Msa0521290:intron	30.0%
ACTTTCCTCTTAGATATTGT+GGG	+	chr3_4:73952141-73952160	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	73950610	73954050	73950610	ID=Msa0521290;Name=Msa0521290
chr3_4	mRNA	73950610	73954050	73950610	ID=Msa0521290-mRNA-1;Parent=Msa0521290;Name=Msa0521290-mRNA-1;_AED=0.32;_eAED=0.32;_QI=238\|0.8\|0.66\|1\|0.8\|0.83\|6\|0\|201
chr3_4	exon	73950610	73950695	73950610	ID=Msa0521290-mRNA-1:exon:20909;Parent=Msa0521290-mRNA-1
chr3_4	exon	73951371	73951445	73951371	ID=Msa0521290-mRNA-1:exon:20908;Parent=Msa0521290-mRNA-1
chr3_4	exon	73951571	73951720	73951571	ID=Msa0521290-mRNA-1:exon:20907;Parent=Msa0521290-mRNA-1
chr3_4	exon	73953127	73953219	73953127	ID=Msa0521290-mRNA-1:exon:20906;Parent=Msa0521290-mRNA-1
chr3_4	exon	73953379	73953489	73953379	ID=Msa0521290-mRNA-1:exon:20905;Parent=Msa0521290-mRNA-1
chr3_4	exon	73953722	73954050	73953722	ID=Msa0521290-mRNA-1:exon:20904;Parent=Msa0521290-mRNA-1
chr3_4	five_prime_UTR	73953813	73954050	73953813	ID=Msa0521290-mRNA-1:five_prime_utr;Parent=Msa0521290-mRNA-1
chr3_4	CDS	73953722	73953812	73953722	ID=Msa0521290-mRNA-1:cds;Parent=Msa0521290-mRNA-1
chr3_4	CDS	73953379	73953489	73953379	ID=Msa0521290-mRNA-1:cds;Parent=Msa0521290-mRNA-1
chr3_4	CDS	73953127	73953219	73953127	ID=Msa0521290-mRNA-1:cds;Parent=Msa0521290-mRNA-1
chr3_4	CDS	73951571	73951720	73951571	ID=Msa0521290-mRNA-1:cds;Parent=Msa0521290-mRNA-1
chr3_4	CDS	73951371	73951445	73951371	ID=Msa0521290-mRNA-1:cds;Parent=Msa0521290-mRNA-1
chr3_4	CDS	73950610	73950695	73950610	ID=Msa0521290-mRNA-1:cds;Parent=Msa0521290-mRNA-1

Gene Sequence

>Msa0521290

ATGTTTGCCGAAGAAAATGGTCTTAAGGGTGACCCTAGATTGCAACACATCTCCCAGTCCATCAGAGTCGTCCCTCACTTCCCTAAAAATGGAATAATGTTTCAAGACATAACGACATTGTTGTTAGATCACAAGGCGTTTAAAGATACTGTCGATATCTTTGTCGATCGTTACAGAGACATGCACATTTCAGTTGTTGCTGGAATTGAAGCAAGAGGGTTTGTGTTTGGTTCTGCAGTTGCGTTGGGAATTGGTGCAAAGTTTGTTCCTTTACGCAAACCTCGCAAGCTACCGGGTGAAGTATTTTTCGAAAAATATTCTCTAGAATACGGAACTGATTGTTTAGAAATGCATGTCGGCGCTGTCCAACCTGGTGAACGGGCTATAGTTATCGACGACTTGGTTGCAACAGGTGGAACTTTGTCGGCAGGAATAAAACTTTTAGAACGTGCTAAGGCTGAAGTAGTGGAATGTGCTTGTGTGATTGGTGTGCCTGAGGTTAAGGTATGGCTCAGCTTTTCCTTCTTTTTTCAGGGACGGTGTAAACTTCTTGGAAAGCCACTTTATGTGCTTGTGGAGCCACGTCAAGTAGATGAATGCTTTTGA

Protein sequence

>Msa0521290

MFAEENGLKGDPRLQHISQSIRVVPHFPKNGIMFQDITTLLLDHKAFKDTVDIFVDRYRDMHISVVAGIEARGFVFGSAVALGIGAKFVPLRKPRKLPGEVFFEKYSLEYGTDCLEMHVGAVQPGERAIVIDDLVATGGTLSAGIKLLERAKAEVVECACVIGVPEVKVWLSFSFFFQGRCKLLGKPLYVLVEPRQVDECF*