AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0538170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0538170 MtrunA17_Chr4g0012821 97.500 80 2 0 1 80 1 80 1.36e-50 153
Msa0538170 MtrunA17_Chr8g0379051 84.146 82 11 1 1 80 1 82 2.95e-36 117
Msa0538170 MtrunA17_Chr1g0191471 59.494 79 24 1 10 80 26 104 1.26e-24 89.0
Msa0538170 MtrunA17_Chr2g0326321 60.241 83 27 1 4 80 21 103 1.40e-23 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0538170 AT3G60540.2 84.211 76 11 1 3 77 2 77 7.18e-35 114
Msa0538170 AT3G60540.3 84.211 76 11 1 3 77 2 77 7.18e-35 114
Msa0538170 AT3G60540.1 84.211 76 11 1 3 77 2 77 7.18e-35 114
Msa0538170 AT2G45070.1 78.481 79 13 1 3 77 2 80 5.82e-33 109
Msa0538170 AT2G45070.4 78.481 79 13 1 3 77 2 80 5.82e-33 109
Msa0538170 AT2G45070.2 78.481 79 13 1 3 77 2 80 5.82e-33 109
Msa0538170 AT2G45070.3 78.481 79 13 1 3 77 2 80 5.82e-33 109
Msa0538170 AT5G60460.2 58.333 84 27 1 5 80 22 105 1.92e-22 83.6
Msa0538170 AT5G60460.1 58.333 84 27 1 5 80 22 105 1.92e-22 83.6

Find 43 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGCAGCACTTCCTCTTT+GGG 0.197645 4_1:-17309010 None:intergenic
TAGCTGCAGCACTTCCTCTT+TGG 0.251750 4_1:-17309011 None:intergenic
CTTTGTCAGTACTTAGTAAT+AGG 0.295433 4_1:+17309431 Msa0538170:three_prime_UTR
ACATATCAGGTTGTCATATA+TGG 0.297992 4_1:-17309490 None:intergenic
TGTACCTGAATGAGAAATTC+TGG 0.330483 4_1:-17308017 None:intergenic
GTAACCAGAATTTCTCATTC+AGG 0.341724 4_1:+17308013 Msa0538170:five_prime_UTR
AAACCGGGTGGTGGCGCCTC+TGG 0.346152 4_1:+17309057 Msa0538170:CDS
TCATTTCAGAAAATGGCTTT+AGG 0.348674 4_1:+17308976 Msa0538170:intron
GGCGCCTCTGGAGGAGCAGC+TGG 0.387947 4_1:+17309069 Msa0538170:CDS
TGTACTTGAGGGAGGCTTAA+GGG 0.407052 4_1:+17309211 Msa0538170:CDS
CTGTACTTGAGGGAGGCTTA+AGG 0.429114 4_1:+17309210 Msa0538170:CDS
CAGAAAATGGCTTTAGGTGG+AGG 0.431685 4_1:+17308982 Msa0538170:intron
CCTCCAGAGGCGCCACCACC+CGG 0.444618 4_1:-17309060 None:intergenic
TTCTGGTTACCGAGATTTGT+AGG 0.450826 4_1:-17308000 None:intergenic
TACAATATCAAAGGGATTGG+TGG 0.460120 4_1:-17309319 None:intergenic
GGGATGCAAGGCAAGAAGTT+GGG 0.467110 4_1:+17309231 Msa0538170:three_prime_UTR
ATAAAGTATAACGACATATC+AGG 0.468466 4_1:-17309503 None:intergenic
CGTGGGAAAGCTGTACTTGA+GGG 0.469860 4_1:+17309200 Msa0538170:CDS
CTCATCACAAGAACAACATT+AGG 0.475458 4_1:-17309141 None:intergenic
GTTGTTCTTGTGATGAGCAT+TGG 0.493707 4_1:+17309147 Msa0538170:CDS
AAATACAATATCAAAGGGAT+TGG 0.498381 4_1:-17309322 None:intergenic
TGTTGCCGTCCTTCATGTCG+TGG 0.499572 4_1:+17309182 Msa0538170:CDS
AGGGATGCAAGGCAAGAAGT+TGG 0.502672 4_1:+17309230 Msa0538170:exon
TTTGAGCCCAGGTGCGTCAT+CGG 0.508967 4_1:-17309112 None:intergenic
GCTGCAGCACTTCCTCTTTG+GGG 0.514244 4_1:-17309009 None:intergenic
CTACTGCAAGCATGAGAAGA+AGG 0.514914 4_1:+17309031 Msa0538170:CDS
TTACCGAGATTTGTAGGAGT+TGG 0.523707 4_1:-17307994 None:intergenic
AAAACAAATACAATATCAAA+GGG 0.529969 4_1:-17309327 None:intergenic
GTTGCCGTCCTTCATGTCGT+GGG 0.536584 4_1:+17309183 Msa0538170:CDS
CCGGGTGGTGGCGCCTCTGG+AGG 0.540424 4_1:+17309060 Msa0538170:CDS
TCACCAACTCCTACAAATCT+CGG 0.543845 4_1:+17307991 Msa0538170:five_prime_UTR
TTTCAGAAAATGGCTTTAGG+TGG 0.554792 4_1:+17308979 Msa0538170:intron
AGTTCCAGCTGCTCCTCCAG+AGG 0.584930 4_1:-17309073 None:intergenic
TTCAACAAAAGCAGAAACAT+AGG 0.598294 4_1:-17309392 None:intergenic
TGTCGTTATACTTTATACAC+AGG 0.604631 4_1:+17309510 Msa0538170:three_prime_UTR
AGGGAGGCTTAAGGGATGCA+AGG 0.606445 4_1:+17309219 Msa0538170:exon
GGTGGAGGAGCTCCCCAAAG+AGG 0.606488 4_1:+17308997 Msa0538170:CDS
ACAGCTTTCCCACGACATGA+AGG 0.608814 4_1:-17309191 None:intergenic
TCGTGGGAAAGCTGTACTTG+AGG 0.624214 4_1:+17309199 Msa0538170:CDS
TTTACACCGATGACGCACCT+GGG 0.626430 4_1:+17309106 Msa0538170:CDS
TTGTCATATATGGTTAGCAA+CGG 0.633828 4_1:-17309480 None:intergenic
CTTTCCCACGACATGAAGGA+CGG 0.643668 4_1:-17309187 None:intergenic
GGGAAAGCTGTACTTGAGGG+AGG 0.648270 4_1:+17309203 Msa0538170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAATTATCTAATTTTTAAT+CGG + chr4_1:17308142-17308161 Msa0538170:intron 10.0%
!!! CAAATATGTTATTTTTTATA+TGG + chr4_1:17308633-17308652 Msa0538170:intron 10.0%
!!! ATTAAGAAACTTTTATAAAT+TGG + chr4_1:17308907-17308926 Msa0538170:intron 10.0%
!!! GTAATTATCTAATTTTTAAT+CGG + chr4_1:17308142-17308161 Msa0538170:intron 10.0%
!!! CAAATATGTTATTTTTTATA+TGG + chr4_1:17308633-17308652 Msa0538170:intron 10.0%
!!! ATTAAGAAACTTTTATAAAT+TGG + chr4_1:17308907-17308926 Msa0538170:intron 10.0%
!! TCATAAAAATATCATAAACT+TGG - chr4_1:17308170-17308189 None:intergenic 15.0%
!! TAATAATTTGAATGTCAATT+TGG + chr4_1:17308193-17308212 Msa0538170:intron 15.0%
!! TTTGAGAATATAATGTTTAA+AGG - chr4_1:17308817-17308836 None:intergenic 15.0%
!! AAAACAAATACAATATCAAA+GGG - chr4_1:17309330-17309349 None:intergenic 15.0%
!! AAAAACAAATACAATATCAA+AGG - chr4_1:17309331-17309350 None:intergenic 15.0%
!! TCATAAAAATATCATAAACT+TGG - chr4_1:17308170-17308189 None:intergenic 15.0%
!! TAATAATTTGAATGTCAATT+TGG + chr4_1:17308193-17308212 Msa0538170:intron 15.0%
!! TTTGAGAATATAATGTTTAA+AGG - chr4_1:17308817-17308836 None:intergenic 15.0%
!! AAAACAAATACAATATCAAA+GGG - chr4_1:17309330-17309349 None:intergenic 15.0%
!! AAAAACAAATACAATATCAA+AGG - chr4_1:17309331-17309350 None:intergenic 15.0%
!! AAAACTGTTATAATACTCTT+CGG + chr4_1:17308247-17308266 Msa0538170:intron 20.0%
!!! ATTGGAAGTTAATTTGAATA+AGG + chr4_1:17308925-17308944 Msa0538170:intron 20.0%
!!! TTAATTTGAATAAGGTTTTG+TGG + chr4_1:17308933-17308952 Msa0538170:intron 20.0%
!!! TGTATTTGTTTTTGTTCTTT+CGG + chr4_1:17309338-17309357 Msa0538170:three_prime_UTR 20.0%
!! AAAACTGTTATAATACTCTT+CGG + chr4_1:17308247-17308266 Msa0538170:intron 20.0%
!!! ATTGGAAGTTAATTTGAATA+AGG + chr4_1:17308925-17308944 Msa0538170:intron 20.0%
!!! TTAATTTGAATAAGGTTTTG+TGG + chr4_1:17308933-17308952 Msa0538170:intron 20.0%
!!! TGTATTTGTTTTTGTTCTTT+CGG + chr4_1:17309338-17309357 Msa0538170:three_prime_UTR 20.0%
!! ATCTAAAACTCTTAATTGCA+TGG - chr4_1:17308108-17308127 None:intergenic 25.0%
!! ACAATTCATATCAAGTGTTT+TGG - chr4_1:17308451-17308470 None:intergenic 25.0%
!! TGATATGAATTGTTGCTTAT+TGG + chr4_1:17308458-17308477 Msa0538170:intron 25.0%
! TCTCATAACAATTTGAAAAC+AGG - chr4_1:17308522-17308541 None:intergenic 25.0%
! CTTAATATTGTTGAGAATCA+TGG + chr4_1:17308609-17308628 Msa0538170:intron 25.0%
! TCGAATATGTCTTTAATTGT+CGG + chr4_1:17308659-17308678 Msa0538170:intron 25.0%
!! TGAGTTTTCATTTCAGAAAA+TGG + chr4_1:17308969-17308988 Msa0538170:intron 25.0%
! AAATACAATATCAAAGGGAT+TGG - chr4_1:17309325-17309344 None:intergenic 25.0%
! AAAGTGAAAAACAGAAATCT+TGG + chr4_1:17309457-17309476 Msa0538170:three_prime_UTR 25.0%
! ATAAAGTATAACGACATATC+AGG - chr4_1:17309506-17309525 None:intergenic 25.0%
!! ATCTAAAACTCTTAATTGCA+TGG - chr4_1:17308108-17308127 None:intergenic 25.0%
!! ACAATTCATATCAAGTGTTT+TGG - chr4_1:17308451-17308470 None:intergenic 25.0%
!! TGATATGAATTGTTGCTTAT+TGG + chr4_1:17308458-17308477 Msa0538170:intron 25.0%
! TCTCATAACAATTTGAAAAC+AGG - chr4_1:17308522-17308541 None:intergenic 25.0%
! CTTAATATTGTTGAGAATCA+TGG + chr4_1:17308609-17308628 Msa0538170:intron 25.0%
! TCGAATATGTCTTTAATTGT+CGG + chr4_1:17308659-17308678 Msa0538170:intron 25.0%
!! TGAGTTTTCATTTCAGAAAA+TGG + chr4_1:17308969-17308988 Msa0538170:intron 25.0%
! AAATACAATATCAAAGGGAT+TGG - chr4_1:17309325-17309344 None:intergenic 25.0%
! AAAGTGAAAAACAGAAATCT+TGG + chr4_1:17309457-17309476 Msa0538170:three_prime_UTR 25.0%
! ATAAAGTATAACGACATATC+AGG - chr4_1:17309506-17309525 None:intergenic 25.0%
GTTATAATACTCTTCGGATT+AGG + chr4_1:17308253-17308272 Msa0538170:intron 30.0%
TTCAATATAATCAGACGTGT+CGG - chr4_1:17308328-17308347 None:intergenic 30.0%
ATATGTCTTTAATTGTCGGT+TGG + chr4_1:17308663-17308682 Msa0538170:intron 30.0%
TCATTTCAGAAAATGGCTTT+AGG + chr4_1:17308976-17308995 Msa0538170:intron 30.0%
!! AAGAAGTTGGGTTCATTTTT+GGG + chr4_1:17309243-17309262 Msa0538170:three_prime_UTR 30.0%
TTCAACAAAAGCAGAAACAT+AGG - chr4_1:17309395-17309414 None:intergenic 30.0%
CTTTGTCAGTACTTAGTAAT+AGG + chr4_1:17309431-17309450 Msa0538170:three_prime_UTR 30.0%
TTGTCATATATGGTTAGCAA+CGG - chr4_1:17309483-17309502 None:intergenic 30.0%
! ACATATCAGGTTGTCATATA+TGG - chr4_1:17309493-17309512 None:intergenic 30.0%
TGTCGTTATACTTTATACAC+AGG + chr4_1:17309510-17309529 Msa0538170:three_prime_UTR 30.0%
GTTATAATACTCTTCGGATT+AGG + chr4_1:17308253-17308272 Msa0538170:intron 30.0%
TTCAATATAATCAGACGTGT+CGG - chr4_1:17308328-17308347 None:intergenic 30.0%
ATATGTCTTTAATTGTCGGT+TGG + chr4_1:17308663-17308682 Msa0538170:intron 30.0%
TCATTTCAGAAAATGGCTTT+AGG + chr4_1:17308976-17308995 Msa0538170:intron 30.0%
!! AAGAAGTTGGGTTCATTTTT+GGG + chr4_1:17309243-17309262 Msa0538170:three_prime_UTR 30.0%
TTCAACAAAAGCAGAAACAT+AGG - chr4_1:17309395-17309414 None:intergenic 30.0%
CTTTGTCAGTACTTAGTAAT+AGG + chr4_1:17309431-17309450 Msa0538170:three_prime_UTR 30.0%
TTGTCATATATGGTTAGCAA+CGG - chr4_1:17309483-17309502 None:intergenic 30.0%
! ACATATCAGGTTGTCATATA+TGG - chr4_1:17309493-17309512 None:intergenic 30.0%
TGTCGTTATACTTTATACAC+AGG + chr4_1:17309510-17309529 Msa0538170:three_prime_UTR 30.0%
GTAACCAGAATTTCTCATTC+AGG + chr4_1:17308013-17308032 Msa0538170:five_prime_UTR 35.0%
TGTACCTGAATGAGAAATTC+TGG - chr4_1:17308020-17308039 None:intergenic 35.0%
GATGATAAGTATCTGACACA+AGG - chr4_1:17308284-17308303 None:intergenic 35.0%
!! TTTCAGAAAATGGCTTTAGG+TGG + chr4_1:17308979-17308998 Msa0538170:intron 35.0%
CATGAGAAGAAGGAAAAAAC+CGG + chr4_1:17309041-17309060 Msa0538170:CDS 35.0%
ATGAGAAGAAGGAAAAAACC+GGG + chr4_1:17309042-17309061 Msa0538170:CDS 35.0%
CTCATCACAAGAACAACATT+AGG - chr4_1:17309144-17309163 None:intergenic 35.0%
! CAAGAAGTTGGGTTCATTTT+TGG + chr4_1:17309242-17309261 Msa0538170:three_prime_UTR 35.0%
TACAATATCAAAGGGATTGG+TGG - chr4_1:17309322-17309341 None:intergenic 35.0%
GTAACCAGAATTTCTCATTC+AGG + chr4_1:17308013-17308032 Msa0538170:five_prime_UTR 35.0%
TGTACCTGAATGAGAAATTC+TGG - chr4_1:17308020-17308039 None:intergenic 35.0%
GATGATAAGTATCTGACACA+AGG - chr4_1:17308284-17308303 None:intergenic 35.0%
!! TTTCAGAAAATGGCTTTAGG+TGG + chr4_1:17308979-17308998 Msa0538170:intron 35.0%
CATGAGAAGAAGGAAAAAAC+CGG + chr4_1:17309041-17309060 Msa0538170:CDS 35.0%
ATGAGAAGAAGGAAAAAACC+GGG + chr4_1:17309042-17309061 Msa0538170:CDS 35.0%
CTCATCACAAGAACAACATT+AGG - chr4_1:17309144-17309163 None:intergenic 35.0%
! CAAGAAGTTGGGTTCATTTT+TGG + chr4_1:17309242-17309261 Msa0538170:three_prime_UTR 35.0%
TACAATATCAAAGGGATTGG+TGG - chr4_1:17309322-17309341 None:intergenic 35.0%
TCACCAACTCCTACAAATCT+CGG + chr4_1:17307991-17308010 Msa0538170:five_prime_UTR 40.0%
TTACCGAGATTTGTAGGAGT+TGG - chr4_1:17307997-17308016 None:intergenic 40.0%
! TTCTGGTTACCGAGATTTGT+AGG - chr4_1:17308003-17308022 None:intergenic 40.0%
TTATCATCCTGTTCACCAAC+AGG + chr4_1:17308296-17308315 Msa0538170:intron 40.0%
GATATTGACATGTCTGTGTC+TGG + chr4_1:17308402-17308421 Msa0538170:intron 40.0%
! TTGTCGGTTGGATAGACATA+TGG + chr4_1:17308675-17308694 Msa0538170:intron 40.0%
ATGTCGTCGTTTGATTCATG+TGG + chr4_1:17308714-17308733 Msa0538170:intron 40.0%
GTTGTTCTTGTGATGAGCAT+TGG + chr4_1:17309147-17309166 Msa0538170:CDS 40.0%
TCACCAACTCCTACAAATCT+CGG + chr4_1:17307991-17308010 Msa0538170:five_prime_UTR 40.0%
TTACCGAGATTTGTAGGAGT+TGG - chr4_1:17307997-17308016 None:intergenic 40.0%
! TTCTGGTTACCGAGATTTGT+AGG - chr4_1:17308003-17308022 None:intergenic 40.0%
TTATCATCCTGTTCACCAAC+AGG + chr4_1:17308296-17308315 Msa0538170:intron 40.0%
GATATTGACATGTCTGTGTC+TGG + chr4_1:17308402-17308421 Msa0538170:intron 40.0%
! TTGTCGGTTGGATAGACATA+TGG + chr4_1:17308675-17308694 Msa0538170:intron 40.0%
ATGTCGTCGTTTGATTCATG+TGG + chr4_1:17308714-17308733 Msa0538170:intron 40.0%
GTTGTTCTTGTGATGAGCAT+TGG + chr4_1:17309147-17309166 Msa0538170:CDS 40.0%
ATTGACATGTCTGTGTCTGG+TGG + chr4_1:17308405-17308424 Msa0538170:intron 45.0%
TAAGAAACGCGAGCACACTA+AGG - chr4_1:17308593-17308612 None:intergenic 45.0%
AAGTCCCATCCCAGTAAGTA+TGG - chr4_1:17308744-17308763 None:intergenic 45.0%
ATACTTACTGGGATGGGACT+TGG + chr4_1:17308743-17308762 Msa0538170:intron 45.0%
!! CAGAAAATGGCTTTAGGTGG+AGG + chr4_1:17308982-17309001 Msa0538170:intron 45.0%
CTACTGCAAGCATGAGAAGA+AGG + chr4_1:17309031-17309050 Msa0538170:CDS 45.0%
AGAAGAAGGAAAAAACCGGG+TGG + chr4_1:17309045-17309064 Msa0538170:CDS 45.0%
! TTAGGTGAGATTTTGAGCCC+AGG - chr4_1:17309126-17309145 None:intergenic 45.0%
TGTACTTGAGGGAGGCTTAA+GGG + chr4_1:17309211-17309230 Msa0538170:CDS 45.0%
ATTGACATGTCTGTGTCTGG+TGG + chr4_1:17308405-17308424 Msa0538170:intron 45.0%
TAAGAAACGCGAGCACACTA+AGG - chr4_1:17308593-17308612 None:intergenic 45.0%
AAGTCCCATCCCAGTAAGTA+TGG - chr4_1:17308744-17308763 None:intergenic 45.0%
ATACTTACTGGGATGGGACT+TGG + chr4_1:17308743-17308762 Msa0538170:intron 45.0%
!! CAGAAAATGGCTTTAGGTGG+AGG + chr4_1:17308982-17309001 Msa0538170:intron 45.0%
CTACTGCAAGCATGAGAAGA+AGG + chr4_1:17309031-17309050 Msa0538170:CDS 45.0%
AGAAGAAGGAAAAAACCGGG+TGG + chr4_1:17309045-17309064 Msa0538170:CDS 45.0%
! TTAGGTGAGATTTTGAGCCC+AGG - chr4_1:17309126-17309145 None:intergenic 45.0%
TGTACTTGAGGGAGGCTTAA+GGG + chr4_1:17309211-17309230 Msa0538170:CDS 45.0%
TTAAACCGCAACTGCGACAC+AGG - chr4_1:17308223-17308242 None:intergenic 50.0%
! GTTCTGTCCTGTTGGTGAAC+AGG - chr4_1:17308306-17308325 None:intergenic 50.0%
! ATGTCTGTGTCTGGTGGTGT+CGG + chr4_1:17308411-17308430 Msa0538170:intron 50.0%
ATGTGGCCGACCATACTTAC+TGG + chr4_1:17308731-17308750 Msa0538170:intron 50.0%
TGTGGCCGACCATACTTACT+GGG + chr4_1:17308732-17308751 Msa0538170:intron 50.0%
CCCATCCCAGTAAGTATGGT+CGG - chr4_1:17308740-17308759 None:intergenic 50.0%
CCGACCATACTTACTGGGAT+GGG + chr4_1:17308737-17308756 Msa0538170:intron 50.0%
AGCTGCAGCACTTCCTCTTT+GGG - chr4_1:17309013-17309032 None:intergenic 50.0%
TAGCTGCAGCACTTCCTCTT+TGG - chr4_1:17309014-17309033 None:intergenic 50.0%
AGAAGGAAAAAACCGGGTGG+TGG + chr4_1:17309048-17309067 Msa0538170:CDS 50.0%
! TTTTACACCGATGACGCACC+TGG + chr4_1:17309105-17309124 Msa0538170:CDS 50.0%
TTTACACCGATGACGCACCT+GGG + chr4_1:17309106-17309125 Msa0538170:CDS 50.0%
CTTTCCCACGACATGAAGGA+CGG - chr4_1:17309190-17309209 None:intergenic 50.0%
ACAGCTTTCCCACGACATGA+AGG - chr4_1:17309194-17309213 None:intergenic 50.0%
TCGTGGGAAAGCTGTACTTG+AGG + chr4_1:17309199-17309218 Msa0538170:CDS 50.0%
CGTGGGAAAGCTGTACTTGA+GGG + chr4_1:17309200-17309219 Msa0538170:CDS 50.0%
CTGTACTTGAGGGAGGCTTA+AGG + chr4_1:17309210-17309229 Msa0538170:CDS 50.0%
AGGGATGCAAGGCAAGAAGT+TGG + chr4_1:17309230-17309249 Msa0538170:exon 50.0%
GGGATGCAAGGCAAGAAGTT+GGG + chr4_1:17309231-17309250 Msa0538170:three_prime_UTR 50.0%
TTAAACCGCAACTGCGACAC+AGG - chr4_1:17308223-17308242 None:intergenic 50.0%
! GTTCTGTCCTGTTGGTGAAC+AGG - chr4_1:17308306-17308325 None:intergenic 50.0%
! ATGTCTGTGTCTGGTGGTGT+CGG + chr4_1:17308411-17308430 Msa0538170:intron 50.0%
ATGTGGCCGACCATACTTAC+TGG + chr4_1:17308731-17308750 Msa0538170:intron 50.0%
TGTGGCCGACCATACTTACT+GGG + chr4_1:17308732-17308751 Msa0538170:intron 50.0%
CCCATCCCAGTAAGTATGGT+CGG - chr4_1:17308740-17308759 None:intergenic 50.0%
CCGACCATACTTACTGGGAT+GGG + chr4_1:17308737-17308756 Msa0538170:intron 50.0%
AGCTGCAGCACTTCCTCTTT+GGG - chr4_1:17309013-17309032 None:intergenic 50.0%
TAGCTGCAGCACTTCCTCTT+TGG - chr4_1:17309014-17309033 None:intergenic 50.0%
AGAAGGAAAAAACCGGGTGG+TGG + chr4_1:17309048-17309067 Msa0538170:CDS 50.0%
! TTTTACACCGATGACGCACC+TGG + chr4_1:17309105-17309124 Msa0538170:CDS 50.0%
TTTACACCGATGACGCACCT+GGG + chr4_1:17309106-17309125 Msa0538170:CDS 50.0%
CTTTCCCACGACATGAAGGA+CGG - chr4_1:17309190-17309209 None:intergenic 50.0%
ACAGCTTTCCCACGACATGA+AGG - chr4_1:17309194-17309213 None:intergenic 50.0%
TCGTGGGAAAGCTGTACTTG+AGG + chr4_1:17309199-17309218 Msa0538170:CDS 50.0%
CGTGGGAAAGCTGTACTTGA+GGG + chr4_1:17309200-17309219 Msa0538170:CDS 50.0%
CTGTACTTGAGGGAGGCTTA+AGG + chr4_1:17309210-17309229 Msa0538170:CDS 50.0%
AGGGATGCAAGGCAAGAAGT+TGG + chr4_1:17309230-17309249 Msa0538170:exon 50.0%
GGGATGCAAGGCAAGAAGTT+GGG + chr4_1:17309231-17309250 Msa0538170:three_prime_UTR 50.0%
!! ACGTGTCGGTTCTGTCCTGT+TGG - chr4_1:17308314-17308333 None:intergenic 55.0%
GCCGACCATACTTACTGGGA+TGG + chr4_1:17308736-17308755 Msa0538170:intron 55.0%
! ACTGGGATGGGACTTGGTAG+TGG + chr4_1:17308749-17308768 Msa0538170:intron 55.0%
GCTGCAGCACTTCCTCTTTG+GGG - chr4_1:17309012-17309031 None:intergenic 55.0%
TTTGAGCCCAGGTGCGTCAT+CGG - chr4_1:17309115-17309134 None:intergenic 55.0%
TGTTGCCGTCCTTCATGTCG+TGG + chr4_1:17309182-17309201 Msa0538170:CDS 55.0%
GTTGCCGTCCTTCATGTCGT+GGG + chr4_1:17309183-17309202 Msa0538170:CDS 55.0%
GGGAAAGCTGTACTTGAGGG+AGG + chr4_1:17309203-17309222 Msa0538170:CDS 55.0%
AGGGAGGCTTAAGGGATGCA+AGG + chr4_1:17309219-17309238 Msa0538170:exon 55.0%
!! ACGTGTCGGTTCTGTCCTGT+TGG - chr4_1:17308314-17308333 None:intergenic 55.0%
GCCGACCATACTTACTGGGA+TGG + chr4_1:17308736-17308755 Msa0538170:intron 55.0%
! ACTGGGATGGGACTTGGTAG+TGG + chr4_1:17308749-17308768 Msa0538170:intron 55.0%
GCTGCAGCACTTCCTCTTTG+GGG - chr4_1:17309012-17309031 None:intergenic 55.0%
TTTGAGCCCAGGTGCGTCAT+CGG - chr4_1:17309115-17309134 None:intergenic 55.0%
TGTTGCCGTCCTTCATGTCG+TGG + chr4_1:17309182-17309201 Msa0538170:CDS 55.0%
GTTGCCGTCCTTCATGTCGT+GGG + chr4_1:17309183-17309202 Msa0538170:CDS 55.0%
GGGAAAGCTGTACTTGAGGG+AGG + chr4_1:17309203-17309222 Msa0538170:CDS 55.0%
AGGGAGGCTTAAGGGATGCA+AGG + chr4_1:17309219-17309238 Msa0538170:exon 55.0%
GCATGCCTGTGTCGCAGTTG+CGG + chr4_1:17308215-17308234 Msa0538170:intron 60.0%
AGTTCCAGCTGCTCCTCCAG+AGG - chr4_1:17309076-17309095 None:intergenic 60.0%
GCATGCCTGTGTCGCAGTTG+CGG + chr4_1:17308215-17308234 Msa0538170:intron 60.0%
AGTTCCAGCTGCTCCTCCAG+AGG - chr4_1:17309076-17309095 None:intergenic 60.0%
GGTGGAGGAGCTCCCCAAAG+AGG + chr4_1:17308997-17309016 Msa0538170:CDS 65.0%
GGTGGAGGAGCTCCCCAAAG+AGG + chr4_1:17308997-17309016 Msa0538170:CDS 65.0%
! AAACCGGGTGGTGGCGCCTC+TGG + chr4_1:17309057-17309076 Msa0538170:CDS 70.0%
! AAACCGGGTGGTGGCGCCTC+TGG + chr4_1:17309057-17309076 Msa0538170:CDS 70.0%
!! CCTCCAGAGGCGCCACCACC+CGG - chr4_1:17309063-17309082 None:intergenic 75.0%
! GGCGCCTCTGGAGGAGCAGC+TGG + chr4_1:17309069-17309088 Msa0538170:CDS 75.0%
!! CCTCCAGAGGCGCCACCACC+CGG - chr4_1:17309063-17309082 None:intergenic 75.0%
! GGCGCCTCTGGAGGAGCAGC+TGG + chr4_1:17309069-17309088 Msa0538170:CDS 75.0%
!! CCGGGTGGTGGCGCCTCTGG+AGG + chr4_1:17309060-17309079 Msa0538170:CDS 80.0%
!! CCGGGTGGTGGCGCCTCTGG+AGG + chr4_1:17309060-17309079 Msa0538170:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 17307924 17309541 17307924 ID=Msa0538170;Name=Msa0538170
chr4_1 mRNA 17307924 17309541 17307924 ID=Msa0538170-mRNA-1;Parent=Msa0538170;Name=Msa0538170-mRNA-1;_AED=0.00;_eAED=0.00;_QI=111|0|0.5|1|0|0.5|2|311|80
chr4_1 exon 17307924 17308034 17307924 ID=Msa0538170-mRNA-1:exon:5156;Parent=Msa0538170-mRNA-1
chr4_1 exon 17308988 17309541 17308988 ID=Msa0538170-mRNA-1:exon:5157;Parent=Msa0538170-mRNA-1
chr4_1 five_prime_UTR 17307924 17308034 17307924 ID=Msa0538170-mRNA-1:five_prime_utr;Parent=Msa0538170-mRNA-1
chr4_1 CDS 17308988 17309230 17308988 ID=Msa0538170-mRNA-1:cds;Parent=Msa0538170-mRNA-1
chr4_1 three_prime_UTR 17309231 17309541 17309231 ID=Msa0538170-mRNA-1:three_prime_utr;Parent=Msa0538170-mRNA-1
Gene Sequence

>Msa0538170

ATGGCTTTAGGTGGAGGAGCTCCCCAAAGAGGAAGTGCTGCAGCTACTGCAAGCATGAGAAGAAGGAAAAAACCGGGTGGTGGCGCCTCTGGAGGAGCAGCTGGAACTATGCTTCAATTTTACACCGATGACGCACCTGGGCTCAAAATCTCACCTAATGTTGTTCTTGTGATGAGCATTGGCTTTATAGCTTTTGTTGCCGTCCTTCATGTCGTGGGAAAGCTGTACTTGAGGGAGGCTTAA
Protein sequence

>Msa0538170

MALGGGAPQRGSAAATASMRRRKKPGGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSIGFIAFVAVLHVVGKLYLREA*