AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0691010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0691010 MtrunA17_Chr4g0039921 97.484 159 4 0 1 159 1 159 1.03e-110 311
Msa0691010 MtrunA17_Chr4g0039941 78.750 160 33 1 1 159 1 160 3.52e-87 252
Msa0691010 MtrunA17_Chr3g0100201 38.144 97 58 1 35 129 34 130 2.41e-14 66.2
Msa0691010 MtrunA17_Chr3g0100191 30.693 101 70 0 27 127 26 126 2.83e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 63 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACAGATCGAAAGAATTT+AGG 0.113164 4_4:-58965280 None:intergenic
CAACAGATCGAAAGAATTTA+GGG 0.210794 4_4:-58965279 None:intergenic
TCCACCTTCTGAAATCTTTC+AGG 0.221004 4_4:-58964121 None:intergenic
CTCTTTGGCACTCTTGCTAT+TGG 0.264799 4_4:+58964076 Msa0691010:CDS
TGGTGTTACGTGTCAAGTTT+AGG 0.342391 4_4:+58963883 Msa0691010:five_prime_UTR
TGTAATCATGTTAATCATAA+TGG 0.356867 4_4:+58965174 Msa0691010:three_prime_UTR
GAAAATTTGTTCTTCAACCT+TGG 0.366123 4_4:-58965057 None:intergenic
AGATCCCATGCTTCACTTGC+TGG 0.373899 4_4:-58964055 None:intergenic
CTAGTTTCTTTACATCTTGA+AGG 0.379902 4_4:-58965202 None:intergenic
CTACAATGTCTGCCAATTGC+TGG 0.390692 4_4:-58964815 None:intergenic
TCAGGTTTACCTGGCCCAAC+TGG 0.416975 4_4:+58964585 Msa0691010:intron
TTGAAGGAGATGGAGGGGTT+GGG 0.418155 4_4:+58964149 Msa0691010:CDS
AACACCAGATGTTTATAGAT+AGG 0.424144 4_4:-58963867 None:intergenic
ACTTGTTTCCATCCAGCAAT+TGG 0.424661 4_4:+58964803 Msa0691010:CDS
AGATGTGGTGTATGTGGTGC+GGG 0.436890 4_4:-58963963 None:intergenic
ATAGAGATCAAGAAGATGTT+TGG 0.443361 4_4:+58964001 Msa0691010:exon
AGTGAAGCATGGGATCTCTT+TGG 0.449536 4_4:+58964061 Msa0691010:CDS
TAAGCCTATCTATAAACATC+TGG 0.450623 4_4:+58963863 None:intergenic
TTGCATCCTTGTTTCTGTTG+AGG 0.456610 4_4:-58964854 None:intergenic
ATTGAAGGAGATGGAGGGGT+TGG 0.457658 4_4:+58964148 Msa0691010:CDS
GAGACCTAGGTTCGATACTT+TGG 0.473082 4_4:-58965035 None:intergenic
GGAGTTTGATCAGGTTTACC+TGG 0.477722 4_4:+58964576 Msa0691010:intron
TTGGCAGACATTGTAGAAGT+TGG 0.481902 4_4:+58964822 Msa0691010:CDS
CACACTATTTAGAGTTCGTA+TGG 0.490157 4_4:+58964695 Msa0691010:CDS
CGTATGGAAGTGATAGAGAA+AGG 0.493358 4_4:+58964711 Msa0691010:CDS
CAGAAGGTGGAGCTAATTGA+AGG 0.500124 4_4:+58964133 Msa0691010:CDS
AAAGAAGTGGAGGTAGTTGA+AGG 0.506304 4_4:+58964651 Msa0691010:CDS
TTCTTCAACCTTGGAGACCT+AGG 0.509081 4_4:-58965048 None:intergenic
GTGGAGCTAATTGAAGGAGA+TGG 0.515580 4_4:+58964139 Msa0691010:CDS
AGAGTATGAAGTCAAGGAAG+AGG 0.520181 4_4:+58964764 Msa0691010:CDS
GAATTAATGAAACAAAATGT+TGG 0.520394 4_4:-58964978 None:intergenic
AAGAAGTGGAGGTAGTTGAA+GGG 0.522228 4_4:+58964652 Msa0691010:CDS
TAGTTTCTTTACATCTTGAA+GGG 0.526387 4_4:-58965201 None:intergenic
TGGCACTCTTGCTATTGGAA+AGG 0.531710 4_4:+58964081 Msa0691010:CDS
TACATTAGAGTATGAAGTCA+AGG 0.533592 4_4:+58964758 Msa0691010:CDS
AGAAGTGGAGGTAGTTGAAG+GGG 0.538439 4_4:+58964653 Msa0691010:CDS
GAGCTAATTGAAGGAGATGG+AGG 0.540375 4_4:+58964142 Msa0691010:CDS
CGTGCCAGCAAGTGAAGCAT+GGG 0.555967 4_4:+58964051 Msa0691010:CDS
CTCAAACTCACATTCACTCC+AGG 0.556420 4_4:+58964178 Msa0691010:CDS
TGAAAAGCACATCAAAGAAG+TGG 0.563701 4_4:+58964638 Msa0691010:CDS
ACGTGCCAGCAAGTGAAGCA+TGG 0.570168 4_4:+58964050 Msa0691010:CDS
ATTTGAGAAATAGTACATGT+TGG 0.571917 4_4:-58965101 None:intergenic
TACACAAACTATTTGAGATG+TGG 0.577268 4_4:-58963978 None:intergenic
AGCTAATTGAAGGAGATGGA+GGG 0.580245 4_4:+58964143 Msa0691010:CDS
TTGGTTATCCTAGAATCACT+TGG 0.584194 4_4:-58965082 None:intergenic
GAGATGTGGTGTATGTGGTG+CGG 0.586126 4_4:-58963964 None:intergenic
GCTAATTGAAGGAGATGGAG+GGG 0.586950 4_4:+58964144 Msa0691010:CDS
AATGTCTGCCAATTGCTGGA+TGG 0.587707 4_4:-58964811 None:intergenic
GAAGTGGAGGTAGTTGAAGG+GGG 0.588145 4_4:+58964654 Msa0691010:CDS
TATTTGAGATGTGGTGTATG+TGG 0.603107 4_4:-58963969 None:intergenic
GCCTGAAAGATTTCAGAAGG+TGG 0.605828 4_4:+58964120 Msa0691010:CDS
GTATCGAACCTAGGTCTCCA+AGG 0.614417 4_4:+58965040 Msa0691010:three_prime_UTR
TGCTATTGGAAAGGTCGTTG+AGG 0.616559 4_4:+58964090 Msa0691010:CDS
ATGTTAGAAGTATACTCTCA+AGG 0.617192 4_4:+58965148 Msa0691010:three_prime_UTR
GATGCCTGAAAGATTTCAGA+AGG 0.625914 4_4:+58964117 Msa0691010:CDS
AAAGCACATCAAAGAAGTGG+AGG 0.643108 4_4:+58964641 Msa0691010:CDS
TGTGCCAAAGTATCGAACCT+AGG 0.658410 4_4:+58965031 Msa0691010:three_prime_UTR
GGGAAAGAGAAATTGCACAA+AGG 0.660752 4_4:-58965259 None:intergenic
TATACTTCACATCAAAACTG+TGG 0.675170 4_4:+58963915 Msa0691010:five_prime_UTR
TTGCTGGATGGAAACAAGTG+CGG 0.676967 4_4:-58964799 None:intergenic
CTAGACGTAGCTATCAAGTG+TGG 0.688321 4_4:+58965221 Msa0691010:three_prime_UTR
AAATTACCTCAACAGAAACA+AGG 0.697908 4_4:+58964848 Msa0691010:CDS
GATGTGGTGTATGTGGTGCG+GGG 0.732028 4_4:-58963962 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAAAAAAATATA+TGG - chr4_4:58965007-58965026 None:intergenic 0.0%
!! AAAAAATAAAAAAAAATATA+TGG - chr4_4:58965007-58965026 None:intergenic 0.0%
!!! TAACATAACATTAATTGTTT+TGG + chr4_4:58964551-58964570 Msa0691010:intron 15.0%
!!! TAACATAACATTAATTGTTT+TGG + chr4_4:58964551-58964570 Msa0691010:intron 15.0%
!!! TTATTGTATCATGAACATTT+TGG + chr4_4:58964315-58964334 Msa0691010:intron 20.0%
!! ATGATGAATTAACATACTTA+TGG + chr4_4:58964489-58964508 Msa0691010:intron 20.0%
!! TGATGAATTAACATACTTAT+GGG + chr4_4:58964490-58964509 Msa0691010:intron 20.0%
!!! ATAACATTAATTGTTTTGGT+TGG + chr4_4:58964555-58964574 Msa0691010:intron 20.0%
!! GAATTAATGAAACAAAATGT+TGG - chr4_4:58964981-58965000 None:intergenic 20.0%
!! TGTAATCATGTTAATCATAA+TGG + chr4_4:58965174-58965193 Msa0691010:three_prime_UTR 20.0%
!!! TTATTGTATCATGAACATTT+TGG + chr4_4:58964315-58964334 Msa0691010:intron 20.0%
!! ATGATGAATTAACATACTTA+TGG + chr4_4:58964489-58964508 Msa0691010:intron 20.0%
!! TGATGAATTAACATACTTAT+GGG + chr4_4:58964490-58964509 Msa0691010:intron 20.0%
!!! ATAACATTAATTGTTTTGGT+TGG + chr4_4:58964555-58964574 Msa0691010:intron 20.0%
!! GAATTAATGAAACAAAATGT+TGG - chr4_4:58964981-58965000 None:intergenic 20.0%
!! TGTAATCATGTTAATCATAA+TGG + chr4_4:58965174-58965193 Msa0691010:three_prime_UTR 20.0%
! AATTATTCATACACTACAAG+TGG + chr4_4:58964360-58964379 Msa0691010:intron 25.0%
! AAATATGTCTATGTACAAGT+GGG + chr4_4:58964403-58964422 Msa0691010:intron 25.0%
!! ACTTTTCTTTTAAACCAGTT+GGG - chr4_4:58964602-58964621 None:intergenic 25.0%
!! AACTTTTCTTTTAAACCAGT+TGG - chr4_4:58964603-58964622 None:intergenic 25.0%
! CTAGAGAAAAGAAAAACTTA+TGG + chr4_4:58964917-58964936 Msa0691010:three_prime_UTR 25.0%
! ATTTGAGAAATAGTACATGT+TGG - chr4_4:58965104-58965123 None:intergenic 25.0%
! TAGTTTCTTTACATCTTGAA+GGG - chr4_4:58965204-58965223 None:intergenic 25.0%
! AATTATTCATACACTACAAG+TGG + chr4_4:58964360-58964379 Msa0691010:intron 25.0%
! AAATATGTCTATGTACAAGT+GGG + chr4_4:58964403-58964422 Msa0691010:intron 25.0%
!! ACTTTTCTTTTAAACCAGTT+GGG - chr4_4:58964602-58964621 None:intergenic 25.0%
!! AACTTTTCTTTTAAACCAGT+TGG - chr4_4:58964603-58964622 None:intergenic 25.0%
! CTAGAGAAAAGAAAAACTTA+TGG + chr4_4:58964917-58964936 Msa0691010:three_prime_UTR 25.0%
! ATTTGAGAAATAGTACATGT+TGG - chr4_4:58965104-58965123 None:intergenic 25.0%
! TAGTTTCTTTACATCTTGAA+GGG - chr4_4:58965204-58965223 None:intergenic 25.0%
TATACTTCACATCAAAACTG+TGG + chr4_4:58963915-58963934 Msa0691010:five_prime_UTR 30.0%
! TACACAAACTATTTGAGATG+TGG - chr4_4:58963981-58964000 None:intergenic 30.0%
ATAGAGATCAAGAAGATGTT+TGG + chr4_4:58964001-58964020 Msa0691010:exon 30.0%
CAAATATGTCTATGTACAAG+TGG + chr4_4:58964402-58964421 Msa0691010:intron 30.0%
AATATGTCTATGTACAAGTG+GGG + chr4_4:58964404-58964423 Msa0691010:intron 30.0%
CTATAGATTCATCATGTGTT+TGG + chr4_4:58964446-58964465 Msa0691010:intron 30.0%
! GTGCTTTTCATTATCAATCT+TGG - chr4_4:58964628-58964647 None:intergenic 30.0%
TACATTAGAGTATGAAGTCA+AGG + chr4_4:58964758-58964777 Msa0691010:CDS 30.0%
AAATTACCTCAACAGAAACA+AGG + chr4_4:58964848-58964867 Msa0691010:CDS 30.0%
! GAAAATTTGTTCTTCAACCT+TGG - chr4_4:58965060-58965079 None:intergenic 30.0%
! ATTTTCAACCAAGTGATTCT+AGG + chr4_4:58965074-58965093 Msa0691010:three_prime_UTR 30.0%
! ATGTTAGAAGTATACTCTCA+AGG + chr4_4:58965148-58965167 Msa0691010:three_prime_UTR 30.0%
CTAGTTTCTTTACATCTTGA+AGG - chr4_4:58965205-58965224 None:intergenic 30.0%
CAACAGATCGAAAGAATTTA+GGG - chr4_4:58965282-58965301 None:intergenic 30.0%
ACAACAGATCGAAAGAATTT+AGG - chr4_4:58965283-58965302 None:intergenic 30.0%
TATACTTCACATCAAAACTG+TGG + chr4_4:58963915-58963934 Msa0691010:five_prime_UTR 30.0%
! TACACAAACTATTTGAGATG+TGG - chr4_4:58963981-58964000 None:intergenic 30.0%
ATAGAGATCAAGAAGATGTT+TGG + chr4_4:58964001-58964020 Msa0691010:exon 30.0%
CAAATATGTCTATGTACAAG+TGG + chr4_4:58964402-58964421 Msa0691010:intron 30.0%
AATATGTCTATGTACAAGTG+GGG + chr4_4:58964404-58964423 Msa0691010:intron 30.0%
CTATAGATTCATCATGTGTT+TGG + chr4_4:58964446-58964465 Msa0691010:intron 30.0%
! GTGCTTTTCATTATCAATCT+TGG - chr4_4:58964628-58964647 None:intergenic 30.0%
TACATTAGAGTATGAAGTCA+AGG + chr4_4:58964758-58964777 Msa0691010:CDS 30.0%
AAATTACCTCAACAGAAACA+AGG + chr4_4:58964848-58964867 Msa0691010:CDS 30.0%
! GAAAATTTGTTCTTCAACCT+TGG - chr4_4:58965060-58965079 None:intergenic 30.0%
! ATTTTCAACCAAGTGATTCT+AGG + chr4_4:58965074-58965093 Msa0691010:three_prime_UTR 30.0%
! ATGTTAGAAGTATACTCTCA+AGG + chr4_4:58965148-58965167 Msa0691010:three_prime_UTR 30.0%
CTAGTTTCTTTACATCTTGA+AGG - chr4_4:58965205-58965224 None:intergenic 30.0%
CAACAGATCGAAAGAATTTA+GGG - chr4_4:58965282-58965301 None:intergenic 30.0%
ACAACAGATCGAAAGAATTT+AGG - chr4_4:58965283-58965302 None:intergenic 30.0%
! TATTTGAGATGTGGTGTATG+TGG - chr4_4:58963972-58963991 None:intergenic 35.0%
! GTTTACAGTTGATGTTTACC+TGG - chr4_4:58964199-58964218 None:intergenic 35.0%
CATTTGATATGCATCGTCAA+CGG - chr4_4:58964229-58964248 None:intergenic 35.0%
CTTCATGAGTCTAACTCAAT+TGG - chr4_4:58964263-58964282 None:intergenic 35.0%
ATATGTCTATGTACAAGTGG+GGG + chr4_4:58964405-58964424 Msa0691010:intron 35.0%
! TGAAAAGCACATCAAAGAAG+TGG + chr4_4:58964638-58964657 Msa0691010:CDS 35.0%
! CACACTATTTAGAGTTCGTA+TGG + chr4_4:58964695-58964714 Msa0691010:CDS 35.0%
TTGGTTATCCTAGAATCACT+TGG - chr4_4:58965085-58965104 None:intergenic 35.0%
! TATTTGAGATGTGGTGTATG+TGG - chr4_4:58963972-58963991 None:intergenic 35.0%
! GTTTACAGTTGATGTTTACC+TGG - chr4_4:58964199-58964218 None:intergenic 35.0%
CATTTGATATGCATCGTCAA+CGG - chr4_4:58964229-58964248 None:intergenic 35.0%
CTTCATGAGTCTAACTCAAT+TGG - chr4_4:58964263-58964282 None:intergenic 35.0%
ATATGTCTATGTACAAGTGG+GGG + chr4_4:58964405-58964424 Msa0691010:intron 35.0%
! TGAAAAGCACATCAAAGAAG+TGG + chr4_4:58964638-58964657 Msa0691010:CDS 35.0%
! CACACTATTTAGAGTTCGTA+TGG + chr4_4:58964695-58964714 Msa0691010:CDS 35.0%
TTGGTTATCCTAGAATCACT+TGG - chr4_4:58965085-58965104 None:intergenic 35.0%
!! TGGTGTTACGTGTCAAGTTT+AGG + chr4_4:58963883-58963902 Msa0691010:five_prime_UTR 40.0%
GATGCCTGAAAGATTTCAGA+AGG + chr4_4:58964117-58964136 Msa0691010:CDS 40.0%
!! TCCACCTTCTGAAATCTTTC+AGG - chr4_4:58964124-58964143 None:intergenic 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4_4:58964143-58964162 Msa0691010:CDS 40.0%
!!! GTTTTGGTTGGAGTTTGATC+AGG + chr4_4:58964567-58964586 Msa0691010:intron 40.0%
AAAGCACATCAAAGAAGTGG+AGG + chr4_4:58964641-58964660 Msa0691010:CDS 40.0%
AAAGAAGTGGAGGTAGTTGA+AGG + chr4_4:58964651-58964670 Msa0691010:CDS 40.0%
AAGAAGTGGAGGTAGTTGAA+GGG + chr4_4:58964652-58964671 Msa0691010:CDS 40.0%
CGTATGGAAGTGATAGAGAA+AGG + chr4_4:58964711-58964730 Msa0691010:CDS 40.0%
AGAGTATGAAGTCAAGGAAG+AGG + chr4_4:58964764-58964783 Msa0691010:CDS 40.0%
ACTTGTTTCCATCCAGCAAT+TGG + chr4_4:58964803-58964822 Msa0691010:CDS 40.0%
TTGGCAGACATTGTAGAAGT+TGG + chr4_4:58964822-58964841 Msa0691010:CDS 40.0%
!! TTGCATCCTTGTTTCTGTTG+AGG - chr4_4:58964857-58964876 None:intergenic 40.0%
GGGAAAGAGAAATTGCACAA+AGG - chr4_4:58965262-58965281 None:intergenic 40.0%
!! TGGTGTTACGTGTCAAGTTT+AGG + chr4_4:58963883-58963902 Msa0691010:five_prime_UTR 40.0%
GATGCCTGAAAGATTTCAGA+AGG + chr4_4:58964117-58964136 Msa0691010:CDS 40.0%
!! TCCACCTTCTGAAATCTTTC+AGG - chr4_4:58964124-58964143 None:intergenic 40.0%
AGCTAATTGAAGGAGATGGA+GGG + chr4_4:58964143-58964162 Msa0691010:CDS 40.0%
!!! GTTTTGGTTGGAGTTTGATC+AGG + chr4_4:58964567-58964586 Msa0691010:intron 40.0%
AAAGCACATCAAAGAAGTGG+AGG + chr4_4:58964641-58964660 Msa0691010:CDS 40.0%
AAAGAAGTGGAGGTAGTTGA+AGG + chr4_4:58964651-58964670 Msa0691010:CDS 40.0%
AAGAAGTGGAGGTAGTTGAA+GGG + chr4_4:58964652-58964671 Msa0691010:CDS 40.0%
CGTATGGAAGTGATAGAGAA+AGG + chr4_4:58964711-58964730 Msa0691010:CDS 40.0%
AGAGTATGAAGTCAAGGAAG+AGG + chr4_4:58964764-58964783 Msa0691010:CDS 40.0%
ACTTGTTTCCATCCAGCAAT+TGG + chr4_4:58964803-58964822 Msa0691010:CDS 40.0%
TTGGCAGACATTGTAGAAGT+TGG + chr4_4:58964822-58964841 Msa0691010:CDS 40.0%
!! TTGCATCCTTGTTTCTGTTG+AGG - chr4_4:58964857-58964876 None:intergenic 40.0%
GGGAAAGAGAAATTGCACAA+AGG - chr4_4:58965262-58965281 None:intergenic 40.0%
AGTGAAGCATGGGATCTCTT+TGG + chr4_4:58964061-58964080 Msa0691010:CDS 45.0%
! CTCTTTGGCACTCTTGCTAT+TGG + chr4_4:58964076-58964095 Msa0691010:CDS 45.0%
!! TGGCACTCTTGCTATTGGAA+AGG + chr4_4:58964081-58964100 Msa0691010:CDS 45.0%
TGCTATTGGAAAGGTCGTTG+AGG + chr4_4:58964090-58964109 Msa0691010:CDS 45.0%
GCCTGAAAGATTTCAGAAGG+TGG + chr4_4:58964120-58964139 Msa0691010:CDS 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4_4:58964133-58964152 Msa0691010:CDS 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4_4:58964139-58964158 Msa0691010:CDS 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4_4:58964142-58964161 Msa0691010:CDS 45.0%
GCTAATTGAAGGAGATGGAG+GGG + chr4_4:58964144-58964163 Msa0691010:CDS 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4_4:58964178-58964197 Msa0691010:CDS 45.0%
TTGATATGCATCGTCAACGG+AGG - chr4_4:58964226-58964245 None:intergenic 45.0%
CTATGTACAAGTGGGGGATT+CGG + chr4_4:58964411-58964430 Msa0691010:intron 45.0%
GGAGTTTGATCAGGTTTACC+TGG + chr4_4:58964576-58964595 Msa0691010:intron 45.0%
! TCTTTTAAACCAGTTGGGCC+AGG - chr4_4:58964597-58964616 None:intergenic 45.0%
AGAAGTGGAGGTAGTTGAAG+GGG + chr4_4:58964653-58964672 Msa0691010:CDS 45.0%
TTGCTGGATGGAAACAAGTG+CGG - chr4_4:58964802-58964821 None:intergenic 45.0%
AATGTCTGCCAATTGCTGGA+TGG - chr4_4:58964814-58964833 None:intergenic 45.0%
CTACAATGTCTGCCAATTGC+TGG - chr4_4:58964818-58964837 None:intergenic 45.0%
!! TGTGCCAAAGTATCGAACCT+AGG + chr4_4:58965031-58965050 Msa0691010:three_prime_UTR 45.0%
GAGACCTAGGTTCGATACTT+TGG - chr4_4:58965038-58965057 None:intergenic 45.0%
TTCTTCAACCTTGGAGACCT+AGG - chr4_4:58965051-58965070 None:intergenic 45.0%
CTAGACGTAGCTATCAAGTG+TGG + chr4_4:58965221-58965240 Msa0691010:three_prime_UTR 45.0%
AGTGAAGCATGGGATCTCTT+TGG + chr4_4:58964061-58964080 Msa0691010:CDS 45.0%
! CTCTTTGGCACTCTTGCTAT+TGG + chr4_4:58964076-58964095 Msa0691010:CDS 45.0%
!! TGGCACTCTTGCTATTGGAA+AGG + chr4_4:58964081-58964100 Msa0691010:CDS 45.0%
TGCTATTGGAAAGGTCGTTG+AGG + chr4_4:58964090-58964109 Msa0691010:CDS 45.0%
GCCTGAAAGATTTCAGAAGG+TGG + chr4_4:58964120-58964139 Msa0691010:CDS 45.0%
CAGAAGGTGGAGCTAATTGA+AGG + chr4_4:58964133-58964152 Msa0691010:CDS 45.0%
GTGGAGCTAATTGAAGGAGA+TGG + chr4_4:58964139-58964158 Msa0691010:CDS 45.0%
GAGCTAATTGAAGGAGATGG+AGG + chr4_4:58964142-58964161 Msa0691010:CDS 45.0%
GCTAATTGAAGGAGATGGAG+GGG + chr4_4:58964144-58964163 Msa0691010:CDS 45.0%
CTCAAACTCACATTCACTCC+AGG + chr4_4:58964178-58964197 Msa0691010:CDS 45.0%
TTGATATGCATCGTCAACGG+AGG - chr4_4:58964226-58964245 None:intergenic 45.0%
CTATGTACAAGTGGGGGATT+CGG + chr4_4:58964411-58964430 Msa0691010:intron 45.0%
GGAGTTTGATCAGGTTTACC+TGG + chr4_4:58964576-58964595 Msa0691010:intron 45.0%
! TCTTTTAAACCAGTTGGGCC+AGG - chr4_4:58964597-58964616 None:intergenic 45.0%
AGAAGTGGAGGTAGTTGAAG+GGG + chr4_4:58964653-58964672 Msa0691010:CDS 45.0%
TTGCTGGATGGAAACAAGTG+CGG - chr4_4:58964802-58964821 None:intergenic 45.0%
AATGTCTGCCAATTGCTGGA+TGG - chr4_4:58964814-58964833 None:intergenic 45.0%
CTACAATGTCTGCCAATTGC+TGG - chr4_4:58964818-58964837 None:intergenic 45.0%
!! TGTGCCAAAGTATCGAACCT+AGG + chr4_4:58965031-58965050 Msa0691010:three_prime_UTR 45.0%
GAGACCTAGGTTCGATACTT+TGG - chr4_4:58965038-58965057 None:intergenic 45.0%
TTCTTCAACCTTGGAGACCT+AGG - chr4_4:58965051-58965070 None:intergenic 45.0%
CTAGACGTAGCTATCAAGTG+TGG + chr4_4:58965221-58965240 Msa0691010:three_prime_UTR 45.0%
AGATGTGGTGTATGTGGTGC+GGG - chr4_4:58963966-58963985 None:intergenic 50.0%
GAGATGTGGTGTATGTGGTG+CGG - chr4_4:58963967-58963986 None:intergenic 50.0%
AGATCCCATGCTTCACTTGC+TGG - chr4_4:58964058-58964077 None:intergenic 50.0%
ATTGAAGGAGATGGAGGGGT+TGG + chr4_4:58964148-58964167 Msa0691010:CDS 50.0%
TTGAAGGAGATGGAGGGGTT+GGG + chr4_4:58964149-58964168 Msa0691010:CDS 50.0%
GAAGTGGAGGTAGTTGAAGG+GGG + chr4_4:58964654-58964673 Msa0691010:CDS 50.0%
GAAGGGGGATGCTTAGATCT+TGG + chr4_4:58964669-58964688 Msa0691010:CDS 50.0%
GTATCGAACCTAGGTCTCCA+AGG + chr4_4:58965040-58965059 Msa0691010:three_prime_UTR 50.0%
AGATGTGGTGTATGTGGTGC+GGG - chr4_4:58963966-58963985 None:intergenic 50.0%
GAGATGTGGTGTATGTGGTG+CGG - chr4_4:58963967-58963986 None:intergenic 50.0%
AGATCCCATGCTTCACTTGC+TGG - chr4_4:58964058-58964077 None:intergenic 50.0%
ATTGAAGGAGATGGAGGGGT+TGG + chr4_4:58964148-58964167 Msa0691010:CDS 50.0%
TTGAAGGAGATGGAGGGGTT+GGG + chr4_4:58964149-58964168 Msa0691010:CDS 50.0%
GAAGTGGAGGTAGTTGAAGG+GGG + chr4_4:58964654-58964673 Msa0691010:CDS 50.0%
GAAGGGGGATGCTTAGATCT+TGG + chr4_4:58964669-58964688 Msa0691010:CDS 50.0%
GTATCGAACCTAGGTCTCCA+AGG + chr4_4:58965040-58965059 Msa0691010:three_prime_UTR 50.0%
GATGTGGTGTATGTGGTGCG+GGG - chr4_4:58963965-58963984 None:intergenic 55.0%
ACGTGCCAGCAAGTGAAGCA+TGG + chr4_4:58964050-58964069 Msa0691010:CDS 55.0%
CGTGCCAGCAAGTGAAGCAT+GGG + chr4_4:58964051-58964070 Msa0691010:CDS 55.0%
TCAGGTTTACCTGGCCCAAC+TGG + chr4_4:58964585-58964604 Msa0691010:intron 55.0%
GATGTGGTGTATGTGGTGCG+GGG - chr4_4:58963965-58963984 None:intergenic 55.0%
ACGTGCCAGCAAGTGAAGCA+TGG + chr4_4:58964050-58964069 Msa0691010:CDS 55.0%
CGTGCCAGCAAGTGAAGCAT+GGG + chr4_4:58964051-58964070 Msa0691010:CDS 55.0%
TCAGGTTTACCTGGCCCAAC+TGG + chr4_4:58964585-58964604 Msa0691010:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 58963869 58965320 58963869 ID=Msa0691010;Name=Msa0691010
chr4_4 mRNA 58963869 58965320 58963869 ID=Msa0691010-mRNA-1;Parent=Msa0691010;Name=Msa0691010-mRNA-1;_AED=0.22;_eAED=0.22;_QI=147|1|1|1|1|1|2|436|159
chr4_4 exon 58963869 58964199 58963869 ID=Msa0691010-mRNA-1:exon:15938;Parent=Msa0691010-mRNA-1
chr4_4 exon 58964589 58965320 58964589 ID=Msa0691010-mRNA-1:exon:15939;Parent=Msa0691010-mRNA-1
chr4_4 five_prime_UTR 58963869 58964015 58963869 ID=Msa0691010-mRNA-1:five_prime_utr;Parent=Msa0691010-mRNA-1
chr4_4 CDS 58964016 58964199 58964016 ID=Msa0691010-mRNA-1:cds;Parent=Msa0691010-mRNA-1
chr4_4 CDS 58964589 58964884 58964589 ID=Msa0691010-mRNA-1:cds;Parent=Msa0691010-mRNA-1
chr4_4 three_prime_UTR 58964885 58965320 58964885 ID=Msa0691010-mRNA-1:three_prime_utr;Parent=Msa0691010-mRNA-1
Gene Sequence

>Msa0691010

ATGTTTGGTCAACTTGAGCATGAGTTAGAGCTGCACGTGCCAGCAAGTGAAGCATGGGATCTCTTTGGCACTCTTGCTATTGGAAAGGTCGTTGAGGAACAGATGCCTGAAAGATTTCAGAAGGTGGAGCTAATTGAAGGAGATGGAGGGGTTGGGACTATTCTCAAACTCACATTCACTCCAGGTTTACCTGGCCCAACTGGTTTAAAAGAAAAGTTCACCAAGATTGATAATGAAAAGCACATCAAAGAAGTGGAGGTAGTTGAAGGGGGATGCTTAGATCTTGGATTCACACTATTTAGAGTTCGTATGGAAGTGATAGAGAAAGGTAAAGATTCAAGCATAATCAAAACTACATTAGAGTATGAAGTCAAGGAAGAGGAAGCAGCTAATGCCGCACTTGTTTCCATCCAGCAATTGGCAGACATTGTAGAAGTTGGCAAAAATTACCTCAACAGAAACAAGGATGCAAAAGAATAA
Protein sequence

>Msa0691010

MFGQLEHELELHVPASEAWDLFGTLAIGKVVEEQMPERFQKVELIEGDGGVGTILKLTFTPGLPGPTGLKEKFTKIDNEKHIKEVEVVEGGCLDLGFTLFRVRMEVIEKGKDSSIIKTTLEYEVKEEEAANAALVSIQQLADIVEVGKNYLNRNKDAKE*