AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0555800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555800 MtrunA17_Chr4g0042211 98.758 161 2 0 1 161 1 161 2.77e-113 318
Msa0555800 MtrunA17_Chr2g0280591 63.971 136 48 1 27 161 26 161 8.04e-58 177
Msa0555800 MtrunA17_Chr4g0048861 48.921 139 70 1 5 142 6 144 1.82e-44 143
Msa0555800 MtrunA17_Chr4g0048881 50.376 133 66 0 10 142 12 144 5.25e-44 143
Msa0555800 MtrunA17_Chr4g0048871 49.286 140 69 2 5 142 6 145 1.84e-43 141
Msa0555800 MtrunA17_Chr4g0031861 55.000 120 53 1 27 145 29 148 3.18e-42 138
Msa0555800 MtrunA17_Chr7g0249501 47.934 121 62 1 25 144 37 157 2.46e-37 126
Msa0555800 MtrunA17_Chr7g0234601 44.248 113 63 0 29 141 36 148 7.75e-34 117
Msa0555800 MtrunA17_Chr8g0378501 43.103 116 64 2 27 141 29 143 9.68e-29 103
Msa0555800 MtrunA17_Chr8g0378491 35.652 115 74 0 28 142 29 143 2.96e-24 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555800 AT5G54530.1 62.121 132 50 0 28 159 28 159 1.70e-61 187
Msa0555800 AT1G61667.1 57.576 132 55 1 25 155 18 149 1.40e-52 164
Msa0555800 AT1G61667.2 57.576 132 55 1 25 155 54 185 4.35e-52 164
Msa0555800 AT3G07470.2 40.876 137 77 2 11 143 10 146 1.07e-32 114
Msa0555800 AT3G07470.3 40.876 137 77 2 11 143 10 146 1.31e-32 114
Msa0555800 AT3G07470.1 40.876 137 77 2 11 143 10 146 1.93e-32 114
Msa0555800 AT3G07460.1 40.876 137 79 2 6 141 8 143 4.81e-32 113
Msa0555800 AT3G07460.2 40.876 137 79 2 6 141 8 143 7.26e-31 112
Msa0555800 AT5G16380.1 43.796 137 72 3 10 141 8 144 5.21e-29 105
Msa0555800 AT1G55265.1 39.844 128 75 2 23 149 48 174 8.20e-28 102
Msa0555800 AT5G19860.1 34.711 121 79 0 25 145 32 152 1.18e-22 89.0
Msa0555800 AT1G02813.1 28.058 139 92 4 8 141 1 136 4.83e-11 58.2

Find 61 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATGTTCCTTTCTCTTTA+TGG 0.022892 4_1:+53828483 Msa0555800:exon
AAGGAACATGGAAGGGTTTA+AGG 0.156283 4_1:-53828472 None:intergenic
ATTGGTCTTTCTTGCATATC+TGG 0.216360 4_1:+53830031 Msa0555800:three_prime_UTR
ATGACTATGTTACCCTTCTT+TGG 0.279545 4_1:-53828339 None:intergenic
GTGTAGGACTTTATCTCTTC+TGG 0.280118 4_1:-53828631 None:intergenic
TTGTCATGTTTATATACTTT+TGG 0.301741 4_1:+53829838 Msa0555800:three_prime_UTR
AGAAGAGGAGAGAGAAAGTT+GGG 0.313243 4_1:-53828560 None:intergenic
TCCTACACTTTCTCAGAAAA+TGG 0.342412 4_1:+53828646 Msa0555800:CDS
TTGTGTTTGTGGGTCTTTGA+TGG 0.344987 4_1:-53828303 None:intergenic
ATTGTTGATGATCCATCTTC+TGG 0.353815 4_1:+53828829 Msa0555800:CDS
GGAAGGGTTTAAGGTTAAGT+TGG 0.355475 4_1:-53828463 None:intergenic
CATGAACTTCTTCGTTCAAA+AGG 0.359352 4_1:+53828589 Msa0555800:CDS
CCTTCTTTGGTTGGTGACTT+TGG 0.373102 4_1:-53828326 None:intergenic
GTGACTTTGGTTGTGTTTGT+GGG 0.384133 4_1:-53828313 None:intergenic
GGTGACTTTGGTTGTGTTTG+TGG 0.384303 4_1:-53828314 None:intergenic
GGAAGTCTCATTGGTGTTGA+AGG 0.392093 4_1:+53828760 Msa0555800:CDS
GAGGAAGGAGAGAGGATTTG+AGG 0.414030 4_1:+53829757 Msa0555800:CDS
AGAGGAGAAGGAGGAGAGAG+AGG 0.416690 4_1:-53828534 None:intergenic
TCAACAATGATATCTTTAAC+AGG 0.417805 4_1:-53828814 None:intergenic
AGAGAGAAAGTTGGGGAGAG+AGG 0.420739 4_1:-53828552 None:intergenic
TTTGTTCATTCACCAGCTAT+TGG 0.424105 4_1:+53830013 Msa0555800:three_prime_UTR
CTCACCTATGGAAGTCTCAT+TGG 0.430019 4_1:+53828751 Msa0555800:CDS
GGTTTGATTCTCTTCGACAT+TGG 0.430179 4_1:+53828850 Msa0555800:CDS
AAAGTTGGGGAGAGAGGAGA+AGG 0.432500 4_1:-53828546 None:intergenic
GAAGGGTTTAAGGTTAAGTT+GGG 0.433298 4_1:-53828462 None:intergenic
AAGAAGAGGAGAGAGAAAGT+TGG 0.438219 4_1:-53828561 None:intergenic
CCAAAGTCACCAACCAAAGA+AGG 0.456555 4_1:+53828326 Msa0555800:five_prime_UTR
GTTGGGGAGAGAGGAGAAGG+AGG 0.460848 4_1:-53828543 None:intergenic
AATCATGTGAGGAAGGAGAG+AGG 0.462940 4_1:+53829749 Msa0555800:CDS
CTATGTTACCCTTCTTTGGT+TGG 0.463103 4_1:-53828335 None:intergenic
AACTATAGTCAATATGTGTA+GGG 0.474579 4_1:+53829875 Msa0555800:three_prime_UTR
GTTGAAGAATCATGTGAGGA+AGG 0.477726 4_1:+53829742 Msa0555800:CDS
TTGTTAAACAAGGTGATTCA+AGG 0.482786 4_1:-53828684 None:intergenic
AAACTATAGTCAATATGTGT+AGG 0.490066 4_1:+53829874 Msa0555800:three_prime_UTR
AAGAGAATCAAACCAGAAGA+TGG 0.492142 4_1:-53828841 None:intergenic
TGTTTGAGAGAGAGATATGA+TGG 0.499117 4_1:-53828415 None:intergenic
ATAGAATGCCATAAAGAGAA+AGG 0.502220 4_1:-53828491 None:intergenic
AAGTTCATGGATGGAAGAAG+AGG 0.514641 4_1:-53828575 None:intergenic
AACATGTAAAGATGTGGGTG+GGG 0.517641 4_1:+53829936 Msa0555800:three_prime_UTR
TCTTCTGGTAAGAGACCAGC+TGG 0.524230 4_1:-53828616 None:intergenic
CGTTCAAAAGGCTTACCAGC+TGG 0.528273 4_1:+53828601 Msa0555800:CDS
GAACATGTAAAGATGTGGGT+GGG 0.529395 4_1:+53829935 Msa0555800:three_prime_UTR
ATCACTGCTAATCTCACCTA+TGG 0.534134 4_1:+53828739 Msa0555800:CDS
AGAGAGGATTTGAGGCAGTG+AGG 0.544712 4_1:+53829765 Msa0555800:CDS
TTGAAGCCTGAAAACTGAGT+TGG 0.544762 4_1:-53829800 None:intergenic
CCTTGAGGTTTACAATGAGG+TGG 0.551693 4_1:-53828913 None:intergenic
ATGCAAGAAAGACCAATAGC+TGG 0.569654 4_1:-53830025 None:intergenic
TGAACGAAGAAGTTCATGGA+TGG 0.572953 4_1:-53828584 None:intergenic
GAAGAGGAGAGAGAAAGTTG+GGG 0.580735 4_1:-53828559 None:intergenic
ATTAAGAACATGTAAAGATG+TGG 0.585266 4_1:+53829930 Msa0555800:three_prime_UTR
AAAGAGAAAGGAACATGGAA+GGG 0.586134 4_1:-53828479 None:intergenic
TAAAGAGAAAGGAACATGGA+AGG 0.596020 4_1:-53828480 None:intergenic
CCACCTCATTGTAAACCTCA+AGG 0.602779 4_1:+53828913 Msa0555800:CDS
CAAAGTCACCAACCAAAGAA+GGG 0.606616 4_1:+53828327 Msa0555800:five_prime_UTR
TTACCTTGAGGTTTACAATG+AGG 0.607565 4_1:-53828916 None:intergenic
AGAACATGTAAAGATGTGGG+TGG 0.621050 4_1:+53829934 Msa0555800:three_prime_UTR
GCCATAAAGAGAAAGGAACA+TGG 0.627091 4_1:-53828484 None:intergenic
TTAAGAACATGTAAAGATGT+GGG 0.632160 4_1:+53829931 Msa0555800:three_prime_UTR
ACTATAGTCAATATGTGTAG+GGG 0.656085 4_1:+53829876 Msa0555800:three_prime_UTR
AACACCAATGAGACTTCCAT+AGG 0.677885 4_1:-53828755 None:intergenic
GTGAGTTGAAGAATCATGTG+AGG 0.690883 4_1:+53829738 Msa0555800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTCTTTCCAATTT+TGG + chr4_1:53829141-53829160 Msa0555800:intron 15.0%
!!! TTTTTTTTCTTTCCAATTTT+GGG + chr4_1:53829142-53829161 Msa0555800:intron 15.0%
!!! ATTTGCTTTTAATTATACAA+AGG - chr4_1:53829424-53829443 None:intergenic 15.0%
!! ATATATATAAAAGTCCAAAA+TGG + chr4_1:53829528-53829547 Msa0555800:intron 15.0%
!! TATATATAAAAGTCCAAAAT+GGG + chr4_1:53829529-53829548 Msa0555800:intron 15.0%
!!! TCTTTTTTACTTTTTGTTAA+AGG + chr4_1:53830064-53830083 Msa0555800:three_prime_UTR 15.0%
!!! TTTTTTTTTCTTTCCAATTT+TGG + chr4_1:53829141-53829160 Msa0555800:intron 15.0%
!!! TTTTTTTTCTTTCCAATTTT+GGG + chr4_1:53829142-53829161 Msa0555800:intron 15.0%
!!! ATTTGCTTTTAATTATACAA+AGG - chr4_1:53829424-53829443 None:intergenic 15.0%
!! ATATATATAAAAGTCCAAAA+TGG + chr4_1:53829528-53829547 Msa0555800:intron 15.0%
!! TATATATAAAAGTCCAAAAT+GGG + chr4_1:53829529-53829548 Msa0555800:intron 15.0%
!!! TCTTTTTTACTTTTTGTTAA+AGG + chr4_1:53830064-53830083 Msa0555800:three_prime_UTR 15.0%
!!! TTTTCATACTTTGTTAAACA+AGG - chr4_1:53828697-53828716 None:intergenic 20.0%
!!! TTGGAATTTTGTATTAATTG+AGG + chr4_1:53829193-53829212 Msa0555800:intron 20.0%
!!! TTCAATTTTTTTCATGTTTG+AGG + chr4_1:53829694-53829713 Msa0555800:intron 20.0%
!!! TTGTCATGTTTATATACTTT+TGG + chr4_1:53829838-53829857 Msa0555800:three_prime_UTR 20.0%
!!! TTTTCATACTTTGTTAAACA+AGG - chr4_1:53828697-53828716 None:intergenic 20.0%
!!! TTGGAATTTTGTATTAATTG+AGG + chr4_1:53829193-53829212 Msa0555800:intron 20.0%
!!! TTCAATTTTTTTCATGTTTG+AGG + chr4_1:53829694-53829713 Msa0555800:intron 20.0%
!!! TTGTCATGTTTATATACTTT+TGG + chr4_1:53829838-53829857 Msa0555800:three_prime_UTR 20.0%
! TCAACAATGATATCTTTAAC+AGG - chr4_1:53828817-53828836 None:intergenic 25.0%
!!! TCTAACTTTGTTATTGTCTT+TGG - chr4_1:53829370-53829389 None:intergenic 25.0%
! AATAACAAAGTTAGAAGAGA+AGG + chr4_1:53829375-53829394 Msa0555800:intron 25.0%
! AGAAGGAAAAAATAATTGAG+AGG + chr4_1:53829392-53829411 Msa0555800:intron 25.0%
!! TGTCATTATTTTCAACTTTG+CGG + chr4_1:53829621-53829640 Msa0555800:intron 25.0%
! TATGTGTTGAAAGATGAAAA+TGG + chr4_1:53829651-53829670 Msa0555800:intron 25.0%
! AAACTATAGTCAATATGTGT+AGG + chr4_1:53829874-53829893 Msa0555800:three_prime_UTR 25.0%
! AACTATAGTCAATATGTGTA+GGG + chr4_1:53829875-53829894 Msa0555800:three_prime_UTR 25.0%
! ATTAAGAACATGTAAAGATG+TGG + chr4_1:53829930-53829949 Msa0555800:three_prime_UTR 25.0%
! TTAAGAACATGTAAAGATGT+GGG + chr4_1:53829931-53829950 Msa0555800:three_prime_UTR 25.0%
! TCAACAATGATATCTTTAAC+AGG - chr4_1:53828817-53828836 None:intergenic 25.0%
!!! TCTAACTTTGTTATTGTCTT+TGG - chr4_1:53829370-53829389 None:intergenic 25.0%
! AATAACAAAGTTAGAAGAGA+AGG + chr4_1:53829375-53829394 Msa0555800:intron 25.0%
! AGAAGGAAAAAATAATTGAG+AGG + chr4_1:53829392-53829411 Msa0555800:intron 25.0%
!! TGTCATTATTTTCAACTTTG+CGG + chr4_1:53829621-53829640 Msa0555800:intron 25.0%
! TATGTGTTGAAAGATGAAAA+TGG + chr4_1:53829651-53829670 Msa0555800:intron 25.0%
! AAACTATAGTCAATATGTGT+AGG + chr4_1:53829874-53829893 Msa0555800:three_prime_UTR 25.0%
! AACTATAGTCAATATGTGTA+GGG + chr4_1:53829875-53829894 Msa0555800:three_prime_UTR 25.0%
! ATTAAGAACATGTAAAGATG+TGG + chr4_1:53829930-53829949 Msa0555800:three_prime_UTR 25.0%
! TTAAGAACATGTAAAGATGT+GGG + chr4_1:53829931-53829950 Msa0555800:three_prime_UTR 25.0%
ATAGAATGCCATAAAGAGAA+AGG - chr4_1:53828494-53828513 None:intergenic 30.0%
TTGTTAAACAAGGTGATTCA+AGG - chr4_1:53828687-53828706 None:intergenic 30.0%
! AACAAGAAGAGCTTTTTGTT+TGG + chr4_1:53828788-53828807 Msa0555800:CDS 30.0%
GTTGAACAAAATTTACCTTG+AGG - chr4_1:53828931-53828950 None:intergenic 30.0%
! TATACTCCATGCACAATTTT+GGG + chr4_1:53829026-53829045 Msa0555800:intron 30.0%
AGCAAAAACAGTAGATTGAA+AGG - chr4_1:53829088-53829107 None:intergenic 30.0%
AACAAAACATCTCCCAAAAT+TGG - chr4_1:53829157-53829176 None:intergenic 30.0%
ATATAAAAGTCCAAAATGGG+TGG + chr4_1:53829532-53829551 Msa0555800:intron 30.0%
TATAAAAGTCCAAAATGGGT+GGG + chr4_1:53829533-53829552 Msa0555800:intron 30.0%
!! TTTTCATGTTTGAGGCTAAT+TGG + chr4_1:53829702-53829721 Msa0555800:intron 30.0%
ACTATAGTCAATATGTGTAG+GGG + chr4_1:53829876-53829895 Msa0555800:three_prime_UTR 30.0%
!!! GTTTTTTAGTGTAAAGGTTG+AGG + chr4_1:53829898-53829917 Msa0555800:three_prime_UTR 30.0%
ATAGAATGCCATAAAGAGAA+AGG - chr4_1:53828494-53828513 None:intergenic 30.0%
TTGTTAAACAAGGTGATTCA+AGG - chr4_1:53828687-53828706 None:intergenic 30.0%
! AACAAGAAGAGCTTTTTGTT+TGG + chr4_1:53828788-53828807 Msa0555800:CDS 30.0%
GTTGAACAAAATTTACCTTG+AGG - chr4_1:53828931-53828950 None:intergenic 30.0%
! TATACTCCATGCACAATTTT+GGG + chr4_1:53829026-53829045 Msa0555800:intron 30.0%
AGCAAAAACAGTAGATTGAA+AGG - chr4_1:53829088-53829107 None:intergenic 30.0%
AACAAAACATCTCCCAAAAT+TGG - chr4_1:53829157-53829176 None:intergenic 30.0%
ATATAAAAGTCCAAAATGGG+TGG + chr4_1:53829532-53829551 Msa0555800:intron 30.0%
TATAAAAGTCCAAAATGGGT+GGG + chr4_1:53829533-53829552 Msa0555800:intron 30.0%
!! TTTTCATGTTTGAGGCTAAT+TGG + chr4_1:53829702-53829721 Msa0555800:intron 30.0%
ACTATAGTCAATATGTGTAG+GGG + chr4_1:53829876-53829895 Msa0555800:three_prime_UTR 30.0%
!!! GTTTTTTAGTGTAAAGGTTG+AGG + chr4_1:53829898-53829917 Msa0555800:three_prime_UTR 30.0%
ATGACTATGTTACCCTTCTT+TGG - chr4_1:53828342-53828361 None:intergenic 35.0%
TGTTTGAGAGAGAGATATGA+TGG - chr4_1:53828418-53828437 None:intergenic 35.0%
! GAAGGGTTTAAGGTTAAGTT+GGG - chr4_1:53828465-53828484 None:intergenic 35.0%
AAAGAGAAAGGAACATGGAA+GGG - chr4_1:53828482-53828501 None:intergenic 35.0%
TAAAGAGAAAGGAACATGGA+AGG - chr4_1:53828483-53828502 None:intergenic 35.0%
TCCATGTTCCTTTCTCTTTA+TGG + chr4_1:53828483-53828502 Msa0555800:exon 35.0%
! CTTTTGAACGAAGAAGTTCA+TGG - chr4_1:53828591-53828610 None:intergenic 35.0%
CATGAACTTCTTCGTTCAAA+AGG + chr4_1:53828589-53828608 Msa0555800:CDS 35.0%
TCCTACACTTTCTCAGAAAA+TGG + chr4_1:53828646-53828665 Msa0555800:CDS 35.0%
!! ACCATTTTCTGAGAAAGTGT+AGG - chr4_1:53828650-53828669 None:intergenic 35.0%
!! ATTGTTGATGATCCATCTTC+TGG + chr4_1:53828829-53828848 Msa0555800:CDS 35.0%
AAGAGAATCAAACCAGAAGA+TGG - chr4_1:53828844-53828863 None:intergenic 35.0%
TTACCTTGAGGTTTACAATG+AGG - chr4_1:53828919-53828938 None:intergenic 35.0%
ATGGAGTATAGTTCCAAAAG+GGG - chr4_1:53829016-53829035 None:intergenic 35.0%
CATGGAGTATAGTTCCAAAA+GGG - chr4_1:53829017-53829036 None:intergenic 35.0%
! CTATACTCCATGCACAATTT+TGG + chr4_1:53829025-53829044 Msa0555800:intron 35.0%
TTATGACCCAAAATTGTGCA+TGG - chr4_1:53829035-53829054 None:intergenic 35.0%
GTTGAGTGTGAAATGTTCTT+TGG + chr4_1:53829174-53829193 Msa0555800:intron 35.0%
ATAAAAGTCCAAAATGGGTG+GGG + chr4_1:53829534-53829553 Msa0555800:intron 35.0%
!!! GTAGGGGTTTTTTAGTGTAA+AGG + chr4_1:53829892-53829911 Msa0555800:three_prime_UTR 35.0%
! TTTTAGTGTAAAGGTTGAGG+AGG + chr4_1:53829901-53829920 Msa0555800:three_prime_UTR 35.0%
TTTGTTCATTCACCAGCTAT+TGG + chr4_1:53830013-53830032 Msa0555800:three_prime_UTR 35.0%
!! ATTGGTCTTTCTTGCATATC+TGG + chr4_1:53830031-53830050 Msa0555800:three_prime_UTR 35.0%
ATGACTATGTTACCCTTCTT+TGG - chr4_1:53828342-53828361 None:intergenic 35.0%
TGTTTGAGAGAGAGATATGA+TGG - chr4_1:53828418-53828437 None:intergenic 35.0%
! GAAGGGTTTAAGGTTAAGTT+GGG - chr4_1:53828465-53828484 None:intergenic 35.0%
AAAGAGAAAGGAACATGGAA+GGG - chr4_1:53828482-53828501 None:intergenic 35.0%
TAAAGAGAAAGGAACATGGA+AGG - chr4_1:53828483-53828502 None:intergenic 35.0%
TCCATGTTCCTTTCTCTTTA+TGG + chr4_1:53828483-53828502 Msa0555800:exon 35.0%
! CTTTTGAACGAAGAAGTTCA+TGG - chr4_1:53828591-53828610 None:intergenic 35.0%
CATGAACTTCTTCGTTCAAA+AGG + chr4_1:53828589-53828608 Msa0555800:CDS 35.0%
TCCTACACTTTCTCAGAAAA+TGG + chr4_1:53828646-53828665 Msa0555800:CDS 35.0%
!! ACCATTTTCTGAGAAAGTGT+AGG - chr4_1:53828650-53828669 None:intergenic 35.0%
!! ATTGTTGATGATCCATCTTC+TGG + chr4_1:53828829-53828848 Msa0555800:CDS 35.0%
AAGAGAATCAAACCAGAAGA+TGG - chr4_1:53828844-53828863 None:intergenic 35.0%
TTACCTTGAGGTTTACAATG+AGG - chr4_1:53828919-53828938 None:intergenic 35.0%
ATGGAGTATAGTTCCAAAAG+GGG - chr4_1:53829016-53829035 None:intergenic 35.0%
CATGGAGTATAGTTCCAAAA+GGG - chr4_1:53829017-53829036 None:intergenic 35.0%
! CTATACTCCATGCACAATTT+TGG + chr4_1:53829025-53829044 Msa0555800:intron 35.0%
TTATGACCCAAAATTGTGCA+TGG - chr4_1:53829035-53829054 None:intergenic 35.0%
GTTGAGTGTGAAATGTTCTT+TGG + chr4_1:53829174-53829193 Msa0555800:intron 35.0%
ATAAAAGTCCAAAATGGGTG+GGG + chr4_1:53829534-53829553 Msa0555800:intron 35.0%
!!! GTAGGGGTTTTTTAGTGTAA+AGG + chr4_1:53829892-53829911 Msa0555800:three_prime_UTR 35.0%
! TTTTAGTGTAAAGGTTGAGG+AGG + chr4_1:53829901-53829920 Msa0555800:three_prime_UTR 35.0%
TTTGTTCATTCACCAGCTAT+TGG + chr4_1:53830013-53830032 Msa0555800:three_prime_UTR 35.0%
!! ATTGGTCTTTCTTGCATATC+TGG + chr4_1:53830031-53830050 Msa0555800:three_prime_UTR 35.0%
GTGACTTTGGTTGTGTTTGT+GGG - chr4_1:53828316-53828335 None:intergenic 40.0%
CAAAGTCACCAACCAAAGAA+GGG + chr4_1:53828327-53828346 Msa0555800:five_prime_UTR 40.0%
CTATGTTACCCTTCTTTGGT+TGG - chr4_1:53828338-53828357 None:intergenic 40.0%
! GGAAGGGTTTAAGGTTAAGT+TGG - chr4_1:53828466-53828485 None:intergenic 40.0%
AAGGAACATGGAAGGGTTTA+AGG - chr4_1:53828475-53828494 None:intergenic 40.0%
GCCATAAAGAGAAAGGAACA+TGG - chr4_1:53828487-53828506 None:intergenic 40.0%
AGAAGAGGAGAGAGAAAGTT+GGG - chr4_1:53828563-53828582 None:intergenic 40.0%
AAGAAGAGGAGAGAGAAAGT+TGG - chr4_1:53828564-53828583 None:intergenic 40.0%
AAGTTCATGGATGGAAGAAG+AGG - chr4_1:53828578-53828597 None:intergenic 40.0%
TGAACGAAGAAGTTCATGGA+TGG - chr4_1:53828587-53828606 None:intergenic 40.0%
GTGTAGGACTTTATCTCTTC+TGG - chr4_1:53828634-53828653 None:intergenic 40.0%
ATCACTGCTAATCTCACCTA+TGG + chr4_1:53828739-53828758 Msa0555800:CDS 40.0%
AACACCAATGAGACTTCCAT+AGG - chr4_1:53828758-53828777 None:intergenic 40.0%
GGTTTGATTCTCTTCGACAT+TGG + chr4_1:53828850-53828869 Msa0555800:CDS 40.0%
GCATGGAGTATAGTTCCAAA+AGG - chr4_1:53829018-53829037 None:intergenic 40.0%
! TAAATTAGCCCCACCCATTT+TGG - chr4_1:53829545-53829564 None:intergenic 40.0%
! GTGAGTTGAAGAATCATGTG+AGG + chr4_1:53829738-53829757 Msa0555800:CDS 40.0%
GTTGAAGAATCATGTGAGGA+AGG + chr4_1:53829742-53829761 Msa0555800:CDS 40.0%
! AAGATGCCAACTCAGTTTTC+AGG + chr4_1:53829794-53829813 Msa0555800:three_prime_UTR 40.0%
TTGAAGCCTGAAAACTGAGT+TGG - chr4_1:53829803-53829822 None:intergenic 40.0%
AGAACATGTAAAGATGTGGG+TGG + chr4_1:53829934-53829953 Msa0555800:three_prime_UTR 40.0%
GAACATGTAAAGATGTGGGT+GGG + chr4_1:53829935-53829954 Msa0555800:three_prime_UTR 40.0%
AACATGTAAAGATGTGGGTG+GGG + chr4_1:53829936-53829955 Msa0555800:three_prime_UTR 40.0%
ATGCAAGAAAGACCAATAGC+TGG - chr4_1:53830028-53830047 None:intergenic 40.0%
GTGACTTTGGTTGTGTTTGT+GGG - chr4_1:53828316-53828335 None:intergenic 40.0%
CAAAGTCACCAACCAAAGAA+GGG + chr4_1:53828327-53828346 Msa0555800:five_prime_UTR 40.0%
CTATGTTACCCTTCTTTGGT+TGG - chr4_1:53828338-53828357 None:intergenic 40.0%
! GGAAGGGTTTAAGGTTAAGT+TGG - chr4_1:53828466-53828485 None:intergenic 40.0%
AAGGAACATGGAAGGGTTTA+AGG - chr4_1:53828475-53828494 None:intergenic 40.0%
GCCATAAAGAGAAAGGAACA+TGG - chr4_1:53828487-53828506 None:intergenic 40.0%
AGAAGAGGAGAGAGAAAGTT+GGG - chr4_1:53828563-53828582 None:intergenic 40.0%
AAGAAGAGGAGAGAGAAAGT+TGG - chr4_1:53828564-53828583 None:intergenic 40.0%
AAGTTCATGGATGGAAGAAG+AGG - chr4_1:53828578-53828597 None:intergenic 40.0%
TGAACGAAGAAGTTCATGGA+TGG - chr4_1:53828587-53828606 None:intergenic 40.0%
GTGTAGGACTTTATCTCTTC+TGG - chr4_1:53828634-53828653 None:intergenic 40.0%
ATCACTGCTAATCTCACCTA+TGG + chr4_1:53828739-53828758 Msa0555800:CDS 40.0%
AACACCAATGAGACTTCCAT+AGG - chr4_1:53828758-53828777 None:intergenic 40.0%
GGTTTGATTCTCTTCGACAT+TGG + chr4_1:53828850-53828869 Msa0555800:CDS 40.0%
GCATGGAGTATAGTTCCAAA+AGG - chr4_1:53829018-53829037 None:intergenic 40.0%
! TAAATTAGCCCCACCCATTT+TGG - chr4_1:53829545-53829564 None:intergenic 40.0%
! GTGAGTTGAAGAATCATGTG+AGG + chr4_1:53829738-53829757 Msa0555800:CDS 40.0%
GTTGAAGAATCATGTGAGGA+AGG + chr4_1:53829742-53829761 Msa0555800:CDS 40.0%
! AAGATGCCAACTCAGTTTTC+AGG + chr4_1:53829794-53829813 Msa0555800:three_prime_UTR 40.0%
TTGAAGCCTGAAAACTGAGT+TGG - chr4_1:53829803-53829822 None:intergenic 40.0%
AGAACATGTAAAGATGTGGG+TGG + chr4_1:53829934-53829953 Msa0555800:three_prime_UTR 40.0%
GAACATGTAAAGATGTGGGT+GGG + chr4_1:53829935-53829954 Msa0555800:three_prime_UTR 40.0%
AACATGTAAAGATGTGGGTG+GGG + chr4_1:53829936-53829955 Msa0555800:three_prime_UTR 40.0%
ATGCAAGAAAGACCAATAGC+TGG - chr4_1:53830028-53830047 None:intergenic 40.0%
GGTGACTTTGGTTGTGTTTG+TGG - chr4_1:53828317-53828336 None:intergenic 45.0%
!! CCTTCTTTGGTTGGTGACTT+TGG - chr4_1:53828329-53828348 None:intergenic 45.0%
CCAAAGTCACCAACCAAAGA+AGG + chr4_1:53828326-53828345 Msa0555800:five_prime_UTR 45.0%
GAAGAGGAGAGAGAAAGTTG+GGG - chr4_1:53828562-53828581 None:intergenic 45.0%
CTCACCTATGGAAGTCTCAT+TGG + chr4_1:53828751-53828770 Msa0555800:CDS 45.0%
!! GGAAGTCTCATTGGTGTTGA+AGG + chr4_1:53828760-53828779 Msa0555800:CDS 45.0%
CCTTGAGGTTTACAATGAGG+TGG - chr4_1:53828916-53828935 None:intergenic 45.0%
CCACCTCATTGTAAACCTCA+AGG + chr4_1:53828913-53828932 Msa0555800:CDS 45.0%
! AGATCTGCTACTACCCCTTT+TGG + chr4_1:53829000-53829019 Msa0555800:intron 45.0%
! GCTAATTGGTTGAGTTGTGC+AGG + chr4_1:53829716-53829735 Msa0555800:intron 45.0%
AATCATGTGAGGAAGGAGAG+AGG + chr4_1:53829749-53829768 Msa0555800:CDS 45.0%
GGTGACTTTGGTTGTGTTTG+TGG - chr4_1:53828317-53828336 None:intergenic 45.0%
!! CCTTCTTTGGTTGGTGACTT+TGG - chr4_1:53828329-53828348 None:intergenic 45.0%
CCAAAGTCACCAACCAAAGA+AGG + chr4_1:53828326-53828345 Msa0555800:five_prime_UTR 45.0%
GAAGAGGAGAGAGAAAGTTG+GGG - chr4_1:53828562-53828581 None:intergenic 45.0%
CTCACCTATGGAAGTCTCAT+TGG + chr4_1:53828751-53828770 Msa0555800:CDS 45.0%
!! GGAAGTCTCATTGGTGTTGA+AGG + chr4_1:53828760-53828779 Msa0555800:CDS 45.0%
CCTTGAGGTTTACAATGAGG+TGG - chr4_1:53828916-53828935 None:intergenic 45.0%
CCACCTCATTGTAAACCTCA+AGG + chr4_1:53828913-53828932 Msa0555800:CDS 45.0%
! AGATCTGCTACTACCCCTTT+TGG + chr4_1:53829000-53829019 Msa0555800:intron 45.0%
! GCTAATTGGTTGAGTTGTGC+AGG + chr4_1:53829716-53829735 Msa0555800:intron 45.0%
AATCATGTGAGGAAGGAGAG+AGG + chr4_1:53829749-53829768 Msa0555800:CDS 45.0%
AAAGTTGGGGAGAGAGGAGA+AGG - chr4_1:53828549-53828568 None:intergenic 50.0%
AGAGAGAAAGTTGGGGAGAG+AGG - chr4_1:53828555-53828574 None:intergenic 50.0%
CGTTCAAAAGGCTTACCAGC+TGG + chr4_1:53828601-53828620 Msa0555800:CDS 50.0%
TCTTCTGGTAAGAGACCAGC+TGG - chr4_1:53828619-53828638 None:intergenic 50.0%
GAGGAAGGAGAGAGGATTTG+AGG + chr4_1:53829757-53829776 Msa0555800:CDS 50.0%
! AGAGAGGATTTGAGGCAGTG+AGG + chr4_1:53829765-53829784 Msa0555800:CDS 50.0%
AAAGTTGGGGAGAGAGGAGA+AGG - chr4_1:53828549-53828568 None:intergenic 50.0%
AGAGAGAAAGTTGGGGAGAG+AGG - chr4_1:53828555-53828574 None:intergenic 50.0%
CGTTCAAAAGGCTTACCAGC+TGG + chr4_1:53828601-53828620 Msa0555800:CDS 50.0%
TCTTCTGGTAAGAGACCAGC+TGG - chr4_1:53828619-53828638 None:intergenic 50.0%
GAGGAAGGAGAGAGGATTTG+AGG + chr4_1:53829757-53829776 Msa0555800:CDS 50.0%
! AGAGAGGATTTGAGGCAGTG+AGG + chr4_1:53829765-53829784 Msa0555800:CDS 50.0%
AGAGGAGAAGGAGGAGAGAG+AGG - chr4_1:53828537-53828556 None:intergenic 55.0%
AGAGGAGAAGGAGGAGAGAG+AGG - chr4_1:53828537-53828556 None:intergenic 55.0%
GTTGGGGAGAGAGGAGAAGG+AGG - chr4_1:53828546-53828565 None:intergenic 60.0%
GTTGGGGAGAGAGGAGAAGG+AGG - chr4_1:53828546-53828565 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 53828305 53830208 53828305 ID=Msa0555800;Name=Msa0555800
chr4_1 mRNA 53828305 53830208 53828305 ID=Msa0555800-mRNA-1;Parent=Msa0555800;Name=Msa0555800-mRNA-1;_AED=0.02;_eAED=0.02;_QI=197|1|1|1|1|1|2|418|161
chr4_1 exon 53828305 53828934 53828305 ID=Msa0555800-mRNA-1:exon:15691;Parent=Msa0555800-mRNA-1
chr4_1 exon 53829738 53830208 53829738 ID=Msa0555800-mRNA-1:exon:15692;Parent=Msa0555800-mRNA-1
chr4_1 five_prime_UTR 53828305 53828501 53828305 ID=Msa0555800-mRNA-1:five_prime_utr;Parent=Msa0555800-mRNA-1
chr4_1 CDS 53828502 53828934 53828502 ID=Msa0555800-mRNA-1:cds;Parent=Msa0555800-mRNA-1
chr4_1 CDS 53829738 53829790 53829738 ID=Msa0555800-mRNA-1:cds;Parent=Msa0555800-mRNA-1
chr4_1 three_prime_UTR 53829791 53830208 53829791 ID=Msa0555800-mRNA-1:three_prime_utr;Parent=Msa0555800-mRNA-1
Gene Sequence

>Msa0555800

ATGGCATTCTATAACACTCTTCTCTCTTTCTTCCTCTCTCTCCTCCTTCTCCTCTCTCCCCAACTTTCTCTCTCCTCTTCTTCCATCCATGAACTTCTTCGTTCAAAAGGCTTACCAGCTGGTCTCTTACCAGAAGAGATAAAGTCCTACACTTTCTCAGAAAATGGTCACTTAGAAGTGTTCCTTGAATCACCTTGTTTAACAAAGTATGAAAACAGAGTCTACTTTGAACAAGTTATCACTGCTAATCTCACCTATGGAAGTCTCATTGGTGTTGAAGGTTTGCAACAAGAAGAGCTTTTTGTTTGGTTACCTGTTAAAGATATCATTGTTGATGATCCATCTTCTGGTTTGATTCTCTTCGACATTGGTCTTGCTTATAAACAACTCTCTTTCTCTCTCTTTGAAGTTCCACCTCATTGTAAACCTCAAGGTGAGTTGAAGAATCATGTGAGGAAGGAGAGAGGATTTGAGGCAGTGAGGTAG
Protein sequence

>Msa0555800

MAFYNTLLSFFLSLLLLLSPQLSLSSSSIHELLRSKGLPAGLLPEEIKSYTFSENGHLEVFLESPCLTKYENRVYFEQVITANLTYGSLIGVEGLQQEELFVWLPVKDIIVDDPSSGLILFDIGLAYKQLSFSLFEVPPHCKPQGELKNHVRKERGFEAVR*