AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0431200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431200 MtrunA17_Chr3g0135851 98.652 445 6 0 1 445 1 445 0.0 907
Msa0431200 MtrunA17_Chr8g0342551 44.091 440 218 10 1 432 1 420 8.49e-76 243
Msa0431200 MtrunA17_Chr2g0330051 44.548 431 189 13 1 427 1 385 3.84e-73 235
Msa0431200 MtrunA17_Chr4g0025441 47.122 278 124 6 1 268 1 265 4.95e-58 197
Msa0431200 MtrunA17_Chr6g0462191 39.016 305 151 11 89 380 105 387 2.84e-42 154
Msa0431200 MtrunA17_Chr1g0200161 43.503 177 90 4 90 263 140 309 1.35e-37 143
Msa0431200 MtrunA17_Chr8g0346021 44.041 193 80 5 89 265 125 305 1.11e-36 141
Msa0431200 MtrunA17_Chr7g0263731 42.478 226 106 7 50 265 64 275 2.27e-35 136
Msa0431200 MtrunA17_Chr1g0181581 44.503 191 86 6 72 259 103 276 8.30e-35 135
Msa0431200 MtrunA17_Chr2g0283321 35.600 250 122 8 93 332 65 285 1.07e-32 125
Msa0431200 MtrunA17_Chr2g0316401 35.514 214 127 6 58 270 42 245 4.83e-32 124
Msa0431200 MtrunA17_Chr4g0045641 30.114 352 207 12 56 386 112 445 1.77e-30 123
Msa0431200 MtrunA17_Chr4g0020671 31.466 232 131 5 50 276 60 268 1.38e-28 117
Msa0431200 MtrunA17_Chr1g0146931 39.604 202 101 3 86 267 117 317 1.12e-25 109
Msa0431200 MtrunA17_Chr1g0204901 49.524 105 51 1 90 192 135 239 3.63e-24 104
Msa0431200 MtrunA17_Chr7g0273861 29.947 187 108 3 86 268 120 287 2.49e-21 95.9
Msa0431200 MtrunA17_Chr8g0340761 62.162 74 28 0 85 158 120 193 3.75e-21 95.1
Msa0431200 MtrunA17_Chr3g0126901 38.947 190 108 4 91 279 112 294 6.26e-21 94.7
Msa0431200 MtrunA17_Chr8g0371131 40.000 140 74 3 28 159 2 139 9.95e-21 88.2
Msa0431200 MtrunA17_Chr6g0472551 56.098 82 36 0 86 167 112 193 9.17e-20 91.3
Msa0431200 MtrunA17_Chr8g0389781 63.636 66 24 0 90 155 125 190 1.18e-19 90.9
Msa0431200 MtrunA17_Chr2g0330921 36.628 172 89 5 1 159 1 165 1.43e-18 86.7
Msa0431200 MtrunA17_Chr1g0203711 58.571 70 29 0 90 159 114 183 1.55e-18 87.4
Msa0431200 MtrunA17_Chr4g0060381 33.158 190 97 5 74 262 83 243 4.43e-18 86.7
Msa0431200 MtrunA17_Chr4g0057321 33.511 188 99 6 1 180 1 170 1.22e-17 84.7
Msa0431200 MtrunA17_Chr7g0273951 56.716 67 29 0 93 159 108 174 1.60e-17 84.0
Msa0431200 MtrunA17_Chr1g0166431 47.222 108 52 1 90 192 116 223 4.24e-17 83.2
Msa0431200 MtrunA17_Chr5g0416161 36.290 124 63 3 69 191 99 207 5.59e-13 71.2
Msa0431200 MtrunA17_Chr1g0172611 32.847 137 81 2 93 220 107 241 5.96e-13 70.1
Msa0431200 MtrunA17_Chr1g0156121 49.254 67 34 0 91 157 124 190 1.06e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431200 AT2G26180.1 59.502 442 147 12 1 439 1 413 3.92e-149 431
Msa0431200 AT1G72670.1 45.618 445 189 14 1 436 1 401 1.96e-94 291
Msa0431200 AT1G17480.1 37.277 448 196 14 1 440 1 371 1.66e-65 215
Msa0431200 AT3G22190.3 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa0431200 AT3G22190.2 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa0431200 AT3G22190.4 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa0431200 AT3G22190.1 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa0431200 AT5G03040.1 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa0431200 AT5G03040.3 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa0431200 AT5G03040.2 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa0431200 AT4G00820.1 33.668 398 180 15 89 443 130 486 1.42e-37 144
Msa0431200 AT1G01110.2 31.159 414 228 12 14 385 44 442 2.29e-36 140
Msa0431200 AT3G52290.1 39.674 184 102 2 90 273 107 281 3.91e-35 135
Msa0431200 AT3G15050.1 38.496 226 110 8 91 315 50 247 1.02e-32 125
Msa0431200 AT3G09710.1 38.839 224 108 7 64 269 67 279 1.35e-32 129
Msa0431200 AT3G49260.3 40.513 195 93 4 86 265 115 301 1.94e-32 129
Msa0431200 AT3G49260.1 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa0431200 AT3G49260.4 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa0431200 AT3G49260.2 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa0431200 AT3G09710.2 36.709 237 109 8 64 269 67 293 4.05e-31 125
Msa0431200 AT2G33990.1 37.853 177 99 4 90 265 46 212 1.21e-29 116
Msa0431200 AT4G10640.1 31.864 295 154 9 15 278 22 300 4.70e-27 112
Msa0431200 AT2G26410.2 35.028 177 106 4 89 263 146 315 1.84e-25 109
Msa0431200 AT2G26410.1 35.028 177 106 4 89 263 146 315 1.84e-25 109
Msa0431200 AT5G13460.1 31.897 232 120 8 58 284 86 284 6.25e-23 101
Msa0431200 AT2G43680.4 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa0431200 AT2G43680.2 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa0431200 AT2G43680.3 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa0431200 AT2G43680.5 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa0431200 AT2G43680.1 32.402 179 102 5 77 255 312 471 1.25e-21 98.2
Msa0431200 AT4G14750.1 30.802 237 143 7 1 221 1 232 1.98e-21 95.9
Msa0431200 AT4G14750.3 30.802 237 143 7 1 221 60 291 3.71e-21 95.9
Msa0431200 AT4G14750.2 30.802 237 143 7 1 221 60 291 4.44e-21 95.5
Msa0431200 AT3G59690.2 34.641 153 92 4 81 231 160 306 7.97e-21 95.5
Msa0431200 AT3G59690.1 34.641 153 92 4 81 231 160 306 7.97e-21 95.5
Msa0431200 AT1G51960.1 33.173 208 117 7 1 198 1 196 8.00e-19 87.8
Msa0431200 AT4G23060.1 57.333 75 32 0 91 165 167 241 2.02e-18 87.8
Msa0431200 AT3G16490.1 49.451 91 38 2 90 180 106 188 2.86e-18 86.7
Msa0431200 AT1G18840.1 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa0431200 AT1G18840.3 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa0431200 AT1G18840.2 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa0431200 AT5G07240.1 66.667 63 20 1 90 151 104 166 2.49e-17 84.0
Msa0431200 AT5G07240.2 66.667 63 20 1 90 151 150 212 2.89e-17 84.3
Msa0431200 AT3G51380.1 57.812 64 27 0 89 152 35 98 8.47e-16 73.6
Msa0431200 AT1G14380.6 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa0431200 AT1G14380.5 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa0431200 AT1G14380.2 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa0431200 AT1G14380.7 35.928 167 90 5 1 157 1 160 1.22e-15 79.7
Msa0431200 AT1G74690.1 39.316 117 66 1 62 178 84 195 1.27e-15 79.7
Msa0431200 AT4G29150.1 61.538 65 25 0 89 153 129 193 1.28e-15 79.0
Msa0431200 AT1G14380.1 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa0431200 AT1G14380.3 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa0431200 AT1G14380.4 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa0431200 AT5G03960.3 37.956 137 74 2 93 222 110 242 7.04e-15 76.6
Msa0431200 AT5G03960.2 37.956 137 74 2 93 222 110 242 7.04e-15 76.6
Msa0431200 AT5G03960.1 37.956 137 74 2 93 222 109 241 7.33e-15 76.6
Msa0431200 AT1G01110.1 28.125 288 162 9 134 385 1 279 1.28e-14 75.5
Msa0431200 AT5G62070.1 61.667 60 23 0 86 145 112 171 2.21e-14 75.1
Msa0431200 AT3G49380.1 38.235 136 67 4 19 147 41 166 3.23e-14 74.3
Msa0431200 AT1G19870.1 35.938 128 60 3 47 152 149 276 4.33e-13 72.0
Msa0431200 AT1G19870.2 53.226 62 29 0 91 152 32 93 1.89e-12 69.7
Msa0431200 AT2G02790.2 41.892 74 43 0 84 157 100 173 9.81e-11 64.3
Msa0431200 AT2G02790.3 41.892 74 43 0 84 157 100 173 9.81e-11 64.3

Find 165 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGACGAATTGTCAATTTA+TGG 0.120322 3_2:-83222982 Msa0431200:three_prime_UTR
TCATGGGTGCTTCTGGAAAA+TGG 0.162318 3_2:-83225379 Msa0431200:CDS
TTATTTCTAATAGTAATTTA+TGG 0.163021 3_2:-83222785 Msa0431200:three_prime_UTR
TGTTATACAACATGATTATT+TGG 0.179043 3_2:-83222826 Msa0431200:three_prime_UTR
CAACAAGATGTTGAACTTCT+TGG 0.237700 3_2:+83223149 None:intergenic
AACGAGTAGCTTGTCCAATA+TGG 0.247199 3_2:+83223592 None:intergenic
CGGTGAGACCATGGTGAAAA+AGG 0.254663 3_2:-83223221 Msa0431200:exon
AAGGGTTCTGCAAATTTCTT+TGG 0.258449 3_2:+83223696 None:intergenic
GTTTCGGCATTCACCTGATA+TGG 0.260918 3_2:+83222440 Msa0431190:CDS
TGAGTCAGGTTCATGTAATT+TGG 0.262145 3_2:+83223441 None:intergenic
GCAACACTCATGGGTGCTTC+TGG 0.264318 3_2:-83225386 Msa0431200:exon
TCTAAAGGGCTTTCCATATC+AGG 0.269204 3_2:-83222453 Msa0431200:three_prime_UTR
AACCACTTTCCGCGCCTTCT+TGG 0.292272 3_2:-83224985 Msa0431200:intron
CCATGAGTGTTGCTGAGTTT+TGG 0.299452 3_2:+83225396 None:intergenic
CTTTGTTCATCTCGTGATTC+CGG 0.304293 3_2:+83223829 None:intergenic
CCTTATGCTTTCATACCATT+TGG 0.309982 3_2:+83222333 Msa0431190:CDS
AGCAAGCTGCAGTGACATTA+AGG 0.326388 3_2:-83224549 Msa0431200:CDS
TCTCACCATTAGATGATATA+TGG 0.337909 3_2:-83222693 Msa0431200:three_prime_UTR
CTCAACCTGGAGAGATTATC+TGG 0.341877 3_2:-83223001 Msa0431200:three_prime_UTR
TGGACGAATTGTCAATTTAT+GGG 0.347242 3_2:-83222981 Msa0431200:three_prime_UTR
TGTCTTGTGCATCATAGAAT+TGG 0.353917 3_2:+83222762 None:intergenic
AGCCCTTTAGAACTCGAATA+TGG 0.355019 3_2:+83222465 Msa0431190:CDS
TATACAGAAAATACTTGTTT+CGG 0.356055 3_2:+83222424 Msa0431190:CDS
GCTAAGCCTCCTCCCCATAT+TGG 0.363494 3_2:-83223606 Msa0431200:CDS
AACCAAGGCAAAGCTAAAAT+CGG 0.368576 3_2:-83223415 Msa0431200:CDS
AATTGTTCATTTGTGAGTAT+TGG 0.372492 3_2:-83223029 Msa0431200:three_prime_UTR
TTGCCTTGGTTGATTGAGTC+AGG 0.373865 3_2:+83223427 None:intergenic
GGTGAGATTTATTGATTCAA+TGG 0.373983 3_2:+83222709 None:intergenic
ACATGTTGTTCATCTTTGTC+TGG 0.374417 3_2:+83225329 None:intergenic
AATTTCTTTGGAGGTGGCGG+TGG 0.375706 3_2:+83223708 None:intergenic
GCTTTCATACCATTTGGAAT+TGG 0.378600 3_2:+83222339 Msa0431190:CDS
CCAAATGGTATGAAAGCATA+AGG 0.390674 3_2:-83222333 Msa0431200:three_prime_UTR
CACCGAGCTCTTGTCCGATT+GGG 0.391424 3_2:+83223239 None:intergenic
TGAGGATTCAAGCACTTGTC+AGG 0.392686 3_2:-83224588 Msa0431200:CDS
CACGAGATGAACAAAGCTAA+TGG 0.399444 3_2:-83223822 Msa0431200:CDS
AGAAGGCGCGGAAAGTGGTT+TGG 0.400404 3_2:+83224988 None:intergenic
ACGAGATGAACAAAGCTAAT+GGG 0.406154 3_2:-83223821 Msa0431200:CDS
CAAGTCCAAGTTCCGAGTTT+CGG 0.408556 3_2:-83223563 Msa0431200:CDS
ACCCTTCAGAAGCTGCTTTG+TGG 0.408756 3_2:+83225120 None:intergenic
GCCTTGGGAGACTAGATTGA+TGG 0.409779 3_2:-83223763 Msa0431200:CDS
GATGGAGTTGGTTGGAAAGA+TGG 0.411885 3_2:-83223797 Msa0431200:CDS
TTCCATATTCGAGTTCTAAA+GGG 0.417925 3_2:-83222467 Msa0431200:three_prime_UTR
TGAAAGCTTGATCTCTTGTA+TGG 0.419879 3_2:-83222391 Msa0431200:three_prime_UTR
ATTCCATATTCGAGTTCTAA+AGG 0.422142 3_2:-83222468 Msa0431200:three_prime_UTR
AAAGTAAGAAATGGAGGTTA+TGG 0.428172 3_2:-83225193 Msa0431200:CDS
TCGTACCGAAACTCGGAACT+TGG 0.431873 3_2:+83223558 None:intergenic
TCACCGAGCTCTTGTCCGAT+TGG 0.433938 3_2:+83223238 None:intergenic
CATGGGTGCTTCTGGAAAAT+GGG 0.436014 3_2:-83225378 Msa0431200:CDS
AAATGGGTCAAAGCATTAAT+CGG 0.439295 3_2:-83225362 Msa0431200:CDS
CAAATGAACAATTATACAAA+TGG 0.444321 3_2:+83223039 None:intergenic
AAGGGCTTTGAGGGCATTGA+AGG 0.446119 3_2:-83224617 Msa0431200:CDS
ATTTCTTTGGAGGTGGCGGT+GGG 0.446397 3_2:+83223709 None:intergenic
TCACACCATATATCATCTAA+TGG 0.448162 3_2:+83222688 None:intergenic
ACGAGTAGCTTGTCCAATAT+GGG 0.450987 3_2:+83223593 None:intergenic
TTAGACTTGTTAGGCAAGAA+TGG 0.452196 3_2:-83225025 Msa0431200:CDS
AGCTCAAAGGCAACAGTCAA+TGG 0.457003 3_2:-83223370 Msa0431200:CDS
TGTGAAGTAGAGAGAGAAAG+AGG 0.457973 3_2:+83225509 None:intergenic
TAGACTTGTTAGGCAAGAAT+GGG 0.462512 3_2:-83225024 Msa0431200:CDS
GGTTCTGCAAATTTCTTTGG+AGG 0.465140 3_2:+83223699 None:intergenic
TGCAGGCAAGAAGGGCTTTG+AGG 0.465225 3_2:-83224627 Msa0431200:intron
AGGGTGGTAAAAGTAAGAAA+TGG 0.466097 3_2:-83225202 Msa0431200:CDS
TTTGAGATTTCCAGCAATGC+AGG 0.466329 3_2:-83223899 Msa0431200:intron
GATGGATGGCAGCCAAGCCT+TGG 0.468070 3_2:-83223779 Msa0431200:CDS
CTAACAAGTCTAAAATCCTT+AGG 0.472089 3_2:+83225035 None:intergenic
GCAGGCAAGAAGGGCTTTGA+GGG 0.472599 3_2:-83224626 Msa0431200:intron
TCTGCAAATTTCTTTGGAGG+TGG 0.475452 3_2:+83223702 None:intergenic
TTCAAATTCGAGCTTACAAA+GGG 0.481570 3_2:+83223678 None:intergenic
TCGTCGTGTGAGGATGTCTA+TGG 0.489827 3_2:-83224485 Msa0431200:CDS
CATCAGGAGATAATAGTTCA+TGG 0.490849 3_2:-83225163 Msa0431200:CDS
ACATCCACAACTCCAATGTC+AGG 0.493112 3_2:-83223507 Msa0431200:CDS
GGTGTGACTTATTCATTCAA+TGG 0.499281 3_2:-83222672 Msa0431200:three_prime_UTR
CTTCAGAAGCTGCTTTGTGG+TGG 0.503461 3_2:+83225123 None:intergenic
CGCGAATTAGTGCTTGAGGT+TGG 0.505381 3_2:+83223876 None:intergenic
TGAGTTTCAATTTATGAAGA+GGG 0.508341 3_2:-83223346 Msa0431200:CDS
GCAAATTTCTTTGGAGGTGG+CGG 0.508534 3_2:+83223705 None:intergenic
AGAGGATGAAATATAAACTT+AGG 0.508574 3_2:+83222591 None:intergenic
TGTTTGAAGGTGAAGGAGGG+TGG 0.510225 3_2:-83225218 Msa0431200:intron
ATGGATGGCAGCCAAGCCTT+GGG 0.511965 3_2:-83223778 Msa0431200:CDS
AGGTTATGGAGAAGTTCATC+AGG 0.515436 3_2:-83225179 Msa0431200:CDS
GTCCTTGATGTTACTTGTGA+AGG 0.518061 3_2:-83223068 Msa0431200:three_prime_UTR
AAGTAGAGAGAGAAAGAGGA+GGG 0.518432 3_2:+83225513 None:intergenic
TGACACCATGCTTGAAGTTG+AGG 0.519275 3_2:-83222494 Msa0431200:three_prime_UTR
AAAATTCAAATGAGGCAAGA+AGG 0.523644 3_2:-83224266 Msa0431200:CDS
TGCATACTCACTTGCTCAGA+AGG 0.524622 3_2:-83224219 Msa0431200:intron
ACCAAGGCAAAGCTAAAATC+GGG 0.526058 3_2:-83223414 Msa0431200:CDS
TCACAAGTGTTCCCTGACAT+TGG 0.528516 3_2:+83223495 None:intergenic
CCAAAACTCAGCAACACTCA+TGG 0.532255 3_2:-83225396 Msa0431200:exon
GGTTATGAGTGCAGGCAAGA+AGG 0.532390 3_2:-83224636 Msa0431200:intron
GTTATGAGTGCAGGCAAGAA+GGG 0.535330 3_2:-83224635 Msa0431200:intron
ACTCTCATCGTACCGAAACT+CGG 0.535615 3_2:+83223551 None:intergenic
TTCATTAAGCATATTCTGCA+CGG 0.537002 3_2:+83224453 None:intergenic
CGTTGTTAGAGCTCAGCCTA+AGG 0.538437 3_2:-83225051 Msa0431200:CDS
TACCAAGAAGGCGCGGAAAG+TGG 0.545045 3_2:+83224983 None:intergenic
ACTTCACCTACCAACCCAAT+CGG 0.545649 3_2:-83223253 Msa0431200:CDS
CAATCTAGTCTCCCAAGGCT+TGG 0.546403 3_2:+83223767 None:intergenic
CACCACAAAGCAGCTTCTGA+AGG 0.550489 3_2:-83225122 Msa0431200:CDS
CAAATGGTATGAAAGCATAA+GGG 0.551950 3_2:-83222332 Msa0431200:three_prime_UTR
GTGTAAGCTTCTGCAGCAGT+TGG 0.555988 3_2:+83225089 None:intergenic
TTCGTCCAGATAATCTCTCC+AGG 0.556575 3_2:+83222996 None:intergenic
TTTGAGGGCATTGAAGGCAG+TGG 0.558218 3_2:-83224611 Msa0431200:CDS
AGGAGATAATAGTTCATGGA+AGG 0.563394 3_2:-83225159 Msa0431200:CDS
ACTTATACTTTGAGAGTCAG+AGG 0.565119 3_2:+83222573 None:intergenic
GCTCTAGGACCAATTCCAAA+TGG 0.565328 3_2:-83222348 Msa0431200:three_prime_UTR
TCCTTGATGTTACTTGTGAA+GGG 0.565990 3_2:-83223067 Msa0431200:three_prime_UTR
TTATGTTTGAAGGTGAAGGA+GGG 0.568504 3_2:-83225221 Msa0431200:intron
ATGAGTTTCAATTTATGAAG+AGG 0.569955 3_2:-83223347 Msa0431200:CDS
CGAGATGAACAAAGCTAATG+GGG 0.570107 3_2:-83223820 Msa0431200:CDS
GAGAGTGGAAAATGAGGTGT+TGG 0.571769 3_2:+83223851 None:intergenic
CAAAACTCAGCAACACTCAT+GGG 0.571912 3_2:-83225395 Msa0431200:exon
GAAGTAGAGAGAGAAAGAGG+AGG 0.573417 3_2:+83225512 None:intergenic
GAGCTCTTGTCCGATTGGGT+TGG 0.575108 3_2:+83223243 None:intergenic
AGTATTGGTAATACTCAACC+TGG 0.575609 3_2:-83223014 Msa0431200:three_prime_UTR
GAGTAAGAGCTCGTCGTGTG+AGG 0.575741 3_2:-83224495 Msa0431200:CDS
TTTCAAATTCGAGCTTACAA+AGG 0.575937 3_2:+83223677 None:intergenic
GTTCCCTGACATTGGAGTTG+TGG 0.575976 3_2:+83223503 None:intergenic
CTCGAATTTGAAACCATGCT+CGG 0.576259 3_2:-83223667 Msa0431200:CDS
GCTTGTCCAATATGGGGAGG+AGG 0.576470 3_2:+83223600 None:intergenic
TCTTGTCCGATTGGGTTGGT+AGG 0.578767 3_2:+83223247 None:intergenic
GTGGTAAAAGTAAGAAATGG+AGG 0.581415 3_2:-83225199 Msa0431200:CDS
AGTGACATTAAGGTGCATGC+AGG 0.581722 3_2:-83224539 Msa0431200:CDS
AGGATAGTTGTAACAGCACT+AGG 0.582943 3_2:-83223464 Msa0431200:CDS
TCGTGTGAGGATGTCTATGG+AGG 0.584361 3_2:-83224482 Msa0431200:CDS
TTCTGACCAATGCAAGCTCT+AGG 0.584781 3_2:-83222363 Msa0431200:three_prime_UTR
GATTGGGTTGGTAGGTGAAG+TGG 0.587150 3_2:+83223255 None:intergenic
CATCTCGTGATTCCGGAGAG+TGG 0.591885 3_2:+83223836 None:intergenic
GTGTGATAGCATAGGAACAT+TGG 0.592155 3_2:-83224303 Msa0431200:CDS
AATATGGGGAGGAGGCTTAG+CGG 0.592945 3_2:+83223608 None:intergenic
TCCCTTCACAAGTAACATCA+AGG 0.592979 3_2:+83223066 None:intergenic
AACCCAATCGGACAAGAGCT+CGG 0.593120 3_2:-83223241 Msa0431200:CDS
GAGGATTCAAGCACTTGTCA+GGG 0.594722 3_2:-83224587 Msa0431200:CDS
TTTCTTTGGAGGTGGCGGTG+GGG 0.595890 3_2:+83223710 None:intergenic
ATTGGTCCTAGAGCTTGCAT+TGG 0.600573 3_2:+83222357 Msa0431190:CDS
TTTATGTTTGAAGGTGAAGG+AGG 0.601205 3_2:-83225222 Msa0431200:intron
ATGAACAATTATACAAATGG+AGG 0.601507 3_2:+83223042 None:intergenic
ACCACAAAGCAGCTTCTGAA+GGG 0.605684 3_2:-83225121 Msa0431200:CDS
AGGAGCTTTCAAAAGAGAGA+GGG 0.607085 3_2:-83224246 Msa0431200:CDS
GGAGATAATAGTTCATGGAA+GGG 0.611321 3_2:-83225158 Msa0431200:CDS
AGTGCTGTTACAACTATCCT+CGG 0.615531 3_2:+83223467 None:intergenic
GAAGGGTGGTGTGATAGCAT+AGG 0.616172 3_2:-83224311 Msa0431200:CDS
AGAAGCTTACACTGCTGCAG+TGG 0.619115 3_2:-83225078 Msa0431200:CDS
ACAAGAGCTCGGTGAGACCA+TGG 0.619435 3_2:-83223230 Msa0431200:CDS
TCCATCAATCTAGTCTCCCA+AGG 0.620091 3_2:+83223762 None:intergenic
AAGGAGCTTTCAAAAGAGAG+AGG 0.623020 3_2:-83224247 Msa0431200:CDS
GGGCATTGAAGGCAGTGGTG+AGG 0.626128 3_2:-83224606 Msa0431200:CDS
ATAGTCCTCAACTTCAAGCA+TGG 0.629476 3_2:+83222489 Msa0431190:CDS
ATTCCGGAGAGTGGAAAATG+AGG 0.631071 3_2:+83223845 None:intergenic
CGAGTAGCTTGTCCAATATG+GGG 0.633366 3_2:+83223594 None:intergenic
CGTGTGAGGATGTCTATGGA+GGG 0.633588 3_2:-83224481 Msa0431200:CDS
GAGTTGGTTGGAAAGATGGA+TGG 0.634715 3_2:-83223793 Msa0431200:CDS
TGTCAGGGAACACTTGTGAA+AGG 0.634990 3_2:-83223491 Msa0431200:CDS
GGTTCGCGAATTAGTGCTTG+AGG 0.643363 3_2:+83223872 None:intergenic
AAATGTATAACAGAGCTCAA+AGG 0.643845 3_2:-83223383 Msa0431200:CDS
TTGTGAATCTGCAGGAAGGG+TGG 0.652606 3_2:-83224325 Msa0431200:intron
AAAGGGAAATAGCGGCTGGG+TGG 0.656150 3_2:-83223203 Msa0431200:three_prime_UTR
CATCCACAACTCCAATGTCA+GGG 0.657914 3_2:-83223506 Msa0431200:CDS
GAAAGAGAGACACTTGTGCA+TGG 0.671235 3_2:-83222648 Msa0431200:three_prime_UTR
CTTAGGCTGAGCTCTAACAA+CGG 0.677421 3_2:+83225052 None:intergenic
AATTGAATACCAAGAAGGCG+CGG 0.680101 3_2:+83224976 None:intergenic
AGGATTCAAGCACTTGTCAG+GGG 0.684889 3_2:-83224586 Msa0431200:CDS
TTGTCAGGGGTAGACAAGTG+AGG 0.688660 3_2:-83224573 Msa0431200:CDS
GTAGCTTGTCCAATATGGGG+AGG 0.689343 3_2:+83223597 None:intergenic
GAACCTGACTCAATCAACCA+AGG 0.696026 3_2:-83223430 Msa0431200:CDS
AAAAGAACAATGTCACAACA+AGG 0.709510 3_2:-83223635 Msa0431200:CDS
GAGATGAACAAAGCTAATGG+GGG 0.721370 3_2:-83223819 Msa0431200:CDS
GAACACTTGTGAAAGGACCG+AGG 0.744801 3_2:-83223484 Msa0431200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAATAAACTTTTTTTTGT+GGG + chr3_2:83222589-83222608 None:intergenic 10.0%
!!! AATAAATAAACTTTTTTTTG+TGG + chr3_2:83222590-83222609 None:intergenic 10.0%
!! AAACTAAAAAAACATTAAAT+GGG + chr3_2:83223052-83223071 None:intergenic 10.0%
!! TAAACTAAAAAAACATTAAA+TGG + chr3_2:83223053-83223072 None:intergenic 10.0%
!! TTAAAAAATAACAAATTTGA+GGG + chr3_2:83223736-83223755 None:intergenic 10.0%
!! ATTAAAAAATAACAAATTTG+AGG + chr3_2:83223737-83223756 None:intergenic 10.0%
!!! TTATTTCTAATAGTAATTTA+TGG - chr3_2:83225087-83225106 Msa0431200:CDS 10.0%
!!! AATGTTTTTTTAGTTTAGAT+TGG - chr3_2:83223056-83223075 Msa0431200:three_prime_UTR 15.0%
!! TTAAATCAAAAATTCAAATG+AGG - chr3_2:83223598-83223617 Msa0431200:CDS 15.0%
!! ACAAAATAAAATGAAAAACA+TGG + chr3_2:83224935-83224954 None:intergenic 15.0%
!!! AAGTATAAGTACAATTTTAA+CGG - chr3_2:83225312-83225331 Msa0431200:intron 15.0%
!!! TTATTGTTGTTAACTTTGTT+TGG - chr3_2:83222627-83222646 Msa0431200:three_prime_UTR 20.0%
!! AAAATAAATAGTCATCAAAG+AGG + chr3_2:83222973-83222992 None:intergenic 20.0%
!!! TAGTTTAGATTGGAAAATTT+TGG - chr3_2:83223066-83223085 Msa0431200:three_prime_UTR 20.0%
!! ACTAAATTGAACAATTTGAA+AGG + chr3_2:83223769-83223788 None:intergenic 20.0%
!!! ATAATTTGAAATTTCGTTAG+AGG - chr3_2:83223822-83223841 Msa0431200:CDS 20.0%
!! CTTGGAAAAACAAATAAATA+AGG + chr3_2:83224708-83224727 None:intergenic 20.0%
!! CAAATGAACAATTATACAAA+TGG + chr3_2:83224836-83224855 None:intergenic 20.0%
!! TGTTATACAACATGATTATT+TGG - chr3_2:83225046-83225065 Msa0431200:CDS 20.0%
!! TATACAGAAAATACTTGTTT+CGG + chr3_2:83225451-83225470 None:intergenic 20.0%
!! TTTGTTTGGTTTATGTTTGA+AGG - chr3_2:83222641-83222660 Msa0431200:three_prime_UTR 25.0%
! GAAAAAATTGAATACCAAGA+AGG + chr3_2:83222904-83222923 None:intergenic 25.0%
!! TTTGGACATTCATCTATTTT+AGG - chr3_2:83223084-83223103 Msa0431200:three_prime_UTR 25.0%
!! TTGGACATTCATCTATTTTA+GGG - chr3_2:83223085-83223104 Msa0431200:three_prime_UTR 25.0%
! TAGTGTATTGAACTATTTGT+TGG - chr3_2:83223185-83223204 Msa0431200:three_prime_UTR 25.0%
!!! ATTTTATTTTGTGAATCTGC+AGG - chr3_2:83223539-83223558 Msa0431200:CDS 25.0%
!! GTCTATGAATTTTCAAATCA+TGG - chr3_2:83223799-83223818 Msa0431200:CDS 25.0%
! TATTAGTTCCTAAAGTTGTA+GGG + chr3_2:83223863-83223882 None:intergenic 25.0%
! TTATTAGTTCCTAAAGTTGT+AGG + chr3_2:83223864-83223883 None:intergenic 25.0%
! GTTTATTCATTCAAACATAG+TGG - chr3_2:83223887-83223906 Msa0431200:CDS 25.0%
! GAAAGGAAAGAAAAAGAAAA+AGG + chr3_2:83223932-83223951 None:intergenic 25.0%
! ATGAGTTTCAATTTATGAAG+AGG - chr3_2:83224525-83224544 Msa0431200:CDS 25.0%
! TGAGTTTCAATTTATGAAGA+GGG - chr3_2:83224526-83224545 Msa0431200:CDS 25.0%
! ATGAACAATTATACAAATGG+AGG + chr3_2:83224833-83224852 None:intergenic 25.0%
! AATTGTTCATTTGTGAGTAT+TGG - chr3_2:83224843-83224862 Msa0431200:intron 25.0%
!!! TTTTATTTTGTGAGTGTTCA+TGG - chr3_2:83224944-83224963 Msa0431200:intron 25.0%
! AGAGGATGAAATATAAACTT+AGG + chr3_2:83225284-83225303 None:intergenic 25.0%
! AAATGGGTCAAAGCATTAAT+CGG - chr3_2:83222510-83222529 Msa0431200:three_prime_UTR 30.0%
AAAGTAAGAAATGGAGGTTA+TGG - chr3_2:83222679-83222698 Msa0431200:three_prime_UTR 30.0%
! CTAACAAGTCTAAAATCCTT+AGG + chr3_2:83222840-83222859 None:intergenic 30.0%
!! CTAAGGATTTTAGACTTGTT+AGG - chr3_2:83222838-83222857 Msa0431200:three_prime_UTR 30.0%
!! AAAACAAAAGCTTGAGTTGA+AGG + chr3_2:83222929-83222948 None:intergenic 30.0%
! GTATTGAACTATTTGTTGGA+AGG - chr3_2:83223189-83223208 Msa0431200:three_prime_UTR 30.0%
TGTGTTTGTAATGAATGTGA+GGG - chr3_2:83223215-83223234 Msa0431200:exon 30.0%
TTCATTAAGCATATTCTGCA+CGG + chr3_2:83223422-83223441 None:intergenic 30.0%
AAAATTCAAATGAGGCAAGA+AGG - chr3_2:83223606-83223625 Msa0431200:CDS 30.0%
AGGAAAGAAAAAGAAAAAGG+TGG + chr3_2:83223929-83223948 None:intergenic 30.0%
AAGCATCTTTCTTTGTAGAA+AGG + chr3_2:83223949-83223968 None:intergenic 30.0%
TTCAAATTCGAGCTTACAAA+GGG + chr3_2:83224197-83224216 None:intergenic 30.0%
TTTCAAATTCGAGCTTACAA+AGG + chr3_2:83224198-83224217 None:intergenic 30.0%
AAAAGAACAATGTCACAACA+AGG - chr3_2:83224237-83224256 Msa0431200:CDS 30.0%
AAATGTATAACAGAGCTCAA+AGG - chr3_2:83224489-83224508 Msa0431200:CDS 30.0%
! ATCATTCACTCAACTTTTCT+AGG - chr3_2:83224594-83224613 Msa0431200:CDS 30.0%
TGGACGAATTGTCAATTTAT+GGG - chr3_2:83224891-83224910 Msa0431200:intron 30.0%
TTGATTCAATGGCAAAAACT+AGG + chr3_2:83225155-83225174 None:intergenic 30.0%
!! GGTGAGATTTATTGATTCAA+TGG + chr3_2:83225166-83225185 None:intergenic 30.0%
TCTCACCATTAGATGATATA+TGG - chr3_2:83225179-83225198 Msa0431200:CDS 30.0%
TCACACCATATATCATCTAA+TGG + chr3_2:83225187-83225206 None:intergenic 30.0%
! ATTCCATATTCGAGTTCTAA+AGG - chr3_2:83225404-83225423 Msa0431200:five_prime_UTR 30.0%
!! TTCCATATTCGAGTTCTAAA+GGG - chr3_2:83225405-83225424 Msa0431200:five_prime_UTR 30.0%
! CAAATGGTATGAAAGCATAA+GGG - chr3_2:83225540-83225559 Msa0431200:five_prime_UTR 30.0%
ACATGTTGTTCATCTTTGTC+TGG + chr3_2:83222546-83222565 None:intergenic 35.0%
TGGTTTATGTTTGAAGGTGA+AGG - chr3_2:83222647-83222666 Msa0431200:three_prime_UTR 35.0%
TTTATGTTTGAAGGTGAAGG+AGG - chr3_2:83222650-83222669 Msa0431200:three_prime_UTR 35.0%
TTATGTTTGAAGGTGAAGGA+GGG - chr3_2:83222651-83222670 Msa0431200:three_prime_UTR 35.0%
AGGGTGGTAAAAGTAAGAAA+TGG - chr3_2:83222670-83222689 Msa0431200:three_prime_UTR 35.0%
GTGGTAAAAGTAAGAAATGG+AGG - chr3_2:83222673-83222692 Msa0431200:three_prime_UTR 35.0%
CATCAGGAGATAATAGTTCA+TGG - chr3_2:83222709-83222728 Msa0431200:three_prime_UTR 35.0%
AGGAGATAATAGTTCATGGA+AGG - chr3_2:83222713-83222732 Msa0431200:three_prime_UTR 35.0%
GGAGATAATAGTTCATGGAA+GGG - chr3_2:83222714-83222733 Msa0431200:three_prime_UTR 35.0%
!! TTAGACTTGTTAGGCAAGAA+TGG - chr3_2:83222847-83222866 Msa0431200:three_prime_UTR 35.0%
!! TAGACTTGTTAGGCAAGAAT+GGG - chr3_2:83222848-83222867 Msa0431200:three_prime_UTR 35.0%
CTGTGTTTGTAATGAATGTG+AGG - chr3_2:83223214-83223233 Msa0431200:exon 35.0%
! CAGCAAACTTGAACTTTTGA+AGG - chr3_2:83223447-83223466 Msa0431200:CDS 35.0%
TATGTGTTGTGAATTGACAG+TGG - chr3_2:83223479-83223498 Msa0431200:CDS 35.0%
! TATTTTGTGAATCTGCAGGA+AGG - chr3_2:83223543-83223562 Msa0431200:CDS 35.0%
! ATTTTGTGAATCTGCAGGAA+GGG - chr3_2:83223544-83223563 Msa0431200:CDS 35.0%
! TTAAGTTGCCCTACAACTTT+AGG - chr3_2:83223852-83223871 Msa0431200:CDS 35.0%
ACGAGATGAACAAAGCTAAT+GGG - chr3_2:83224051-83224070 Msa0431200:intron 35.0%
AAGGGTTCTGCAAATTTCTT+TGG + chr3_2:83224179-83224198 None:intergenic 35.0%
TGAGTCAGGTTCATGTAATT+TGG + chr3_2:83224434-83224453 None:intergenic 35.0%
AACCAAGGCAAAGCTAAAAT+CGG - chr3_2:83224457-83224476 Msa0431200:CDS 35.0%
!!! TATGCTTTTAGAATCTCCGT+TGG + chr3_2:83224564-83224583 None:intergenic 35.0%
ATGGTGAAAAAGGGAAATAG+CGG - chr3_2:83224661-83224680 Msa0431200:intron 35.0%
CAACAAGATGTTGAACTTCT+TGG + chr3_2:83224726-83224745 None:intergenic 35.0%
! TCCTTGATGTTACTTGTGAA+GGG - chr3_2:83224805-83224824 Msa0431200:intron 35.0%
!! AGTATTGGTAATACTCAACC+TGG - chr3_2:83224858-83224877 Msa0431200:intron 35.0%
CTGGACGAATTGTCAATTTA+TGG - chr3_2:83224890-83224909 Msa0431200:intron 35.0%
TGTCTTGTGCATCATAGAAT+TGG + chr3_2:83225113-83225132 None:intergenic 35.0%
GGTGTGACTTATTCATTCAA+TGG - chr3_2:83225200-83225219 Msa0431200:CDS 35.0%
! ACTTATACTTTGAGAGTCAG+AGG + chr3_2:83225302-83225321 None:intergenic 35.0%
TGAAAGCTTGATCTCTTGTA+TGG - chr3_2:83225481-83225500 Msa0431200:five_prime_UTR 35.0%
! GCTTTCATACCATTTGGAAT+TGG + chr3_2:83225536-83225555 None:intergenic 35.0%
CCTTATGCTTTCATACCATT+TGG + chr3_2:83225542-83225561 None:intergenic 35.0%
! CCAAATGGTATGAAAGCATA+AGG - chr3_2:83225539-83225558 Msa0431200:five_prime_UTR 35.0%
!! AGAAAAAGAAGTGTTCGTGG+GGG + chr3_2:83222339-83222358 Msa0431190:CDS 40.0%
!! GAGAAAAAGAAGTGTTCGTG+GGG + chr3_2:83222340-83222359 Msa0431190:CDS 40.0%
!! GGAGAAAAAGAAGTGTTCGT+GGG + chr3_2:83222341-83222360 Msa0431190:CDS 40.0%
AAGTAGAGAGAGAAAGAGGA+GGG + chr3_2:83222362-83222381 Msa0431190:CDS 40.0%
TGTGAAGTAGAGAGAGAAAG+AGG + chr3_2:83222366-83222385 Msa0431190:CDS 40.0%
CAAAACTCAGCAACACTCAT+GGG - chr3_2:83222477-83222496 Msa0431200:three_prime_UTR 40.0%
AGGTTATGGAGAAGTTCATC+AGG - chr3_2:83222693-83222712 Msa0431200:three_prime_UTR 40.0%
AATTGAATACCAAGAAGGCG+CGG + chr3_2:83222899-83222918 None:intergenic 40.0%
!! TGAACTTTTGAAGGAAGCAG+AGG - chr3_2:83223456-83223475 Msa0431200:CDS 40.0%
GTGTGATAGCATAGGAACAT+TGG - chr3_2:83223569-83223588 Msa0431200:CDS 40.0%
AAGGAGCTTTCAAAAGAGAG+AGG - chr3_2:83223625-83223644 Msa0431200:CDS 40.0%
AGGAGCTTTCAAAAGAGAGA+GGG - chr3_2:83223626-83223645 Msa0431200:CDS 40.0%
! TCTCTTTTCAGTCCTGAGTA+AGG - chr3_2:83223696-83223715 Msa0431200:CDS 40.0%
! TTTGAGATTTCCAGCAATGC+AGG - chr3_2:83223973-83223992 Msa0431200:intron 40.0%
CTTTGTTCATCTCGTGATTC+CGG + chr3_2:83224046-83224065 None:intergenic 40.0%
CACGAGATGAACAAAGCTAA+TGG - chr3_2:83224050-83224069 Msa0431200:intron 40.0%
CGAGATGAACAAAGCTAATG+GGG - chr3_2:83224052-83224071 Msa0431200:intron 40.0%
GAGATGAACAAAGCTAATGG+GGG - chr3_2:83224053-83224072 Msa0431200:intron 40.0%
!!! TGGGGTTTTTTCTAAGCTCT+CGG + chr3_2:83224147-83224166 None:intergenic 40.0%
! TCTGCAAATTTCTTTGGAGG+TGG + chr3_2:83224173-83224192 None:intergenic 40.0%
GGTTCTGCAAATTTCTTTGG+AGG + chr3_2:83224176-83224195 None:intergenic 40.0%
! CTCGAATTTGAAACCATGCT+CGG - chr3_2:83224205-83224224 Msa0431200:intron 40.0%
!!! TTTTTGACTTTGACCGAGCA+TGG + chr3_2:83224221-83224240 None:intergenic 40.0%
! ACGAGTAGCTTGTCCAATAT+GGG + chr3_2:83224282-83224301 None:intergenic 40.0%
! AACGAGTAGCTTGTCCAATA+TGG + chr3_2:83224283-83224302 None:intergenic 40.0%
! AGTGCTGTTACAACTATCCT+CGG + chr3_2:83224408-83224427 None:intergenic 40.0%
AGGATAGTTGTAACAGCACT+AGG - chr3_2:83224408-83224427 Msa0431200:intron 40.0%
ACCAAGGCAAAGCTAAAATC+GGG - chr3_2:83224458-83224477 Msa0431200:CDS 40.0%
!! GCTATTTCCCTTTTTCACCA+TGG + chr3_2:83224662-83224681 None:intergenic 40.0%
! GTCCTTGATGTTACTTGTGA+AGG - chr3_2:83224804-83224823 Msa0431200:intron 40.0%
TCCCTTCACAAGTAACATCA+AGG + chr3_2:83224809-83224828 None:intergenic 40.0%
!!! GTGAGTGTTCATGGTTTTGT+AGG - chr3_2:83224953-83224972 Msa0431200:intron 40.0%
ATAGTCCTCAACTTCAAGCA+TGG + chr3_2:83225386-83225405 None:intergenic 40.0%
!! AGCCCTTTAGAACTCGAATA+TGG + chr3_2:83225410-83225429 None:intergenic 40.0%
TCTAAAGGGCTTTCCATATC+AGG - chr3_2:83225419-83225438 Msa0431200:five_prime_UTR 40.0%
!! GAAAAAGAAGTGTTCGTGGG+GGG + chr3_2:83222338-83222357 Msa0431190:CDS 45.0%
!! GGGAGAAAAAGAAGTGTTCG+TGG + chr3_2:83222342-83222361 Msa0431190:CDS 45.0%
GAAGTAGAGAGAGAAAGAGG+AGG + chr3_2:83222363-83222382 Msa0431190:CDS 45.0%
AGAGAGAGAGAGAGTGAATG+TGG + chr3_2:83222443-83222462 Msa0431190:CDS 45.0%
!! CCATGAGTGTTGCTGAGTTT+TGG + chr3_2:83222479-83222498 Msa0431190:CDS 45.0%
CCAAAACTCAGCAACACTCA+TGG - chr3_2:83222476-83222495 Msa0431200:three_prime_UTR 45.0%
! TCATGGGTGCTTCTGGAAAA+TGG - chr3_2:83222493-83222512 Msa0431200:three_prime_UTR 45.0%
! CATGGGTGCTTCTGGAAAAT+GGG - chr3_2:83222494-83222513 Msa0431200:three_prime_UTR 45.0%
ACCACAAAGCAGCTTCTGAA+GGG - chr3_2:83222751-83222770 Msa0431200:three_prime_UTR 45.0%
! CTTAGGCTGAGCTCTAACAA+CGG + chr3_2:83222823-83222842 None:intergenic 45.0%
AATGTGAGGGTTATGAGTGC+AGG - chr3_2:83223228-83223247 Msa0431200:CDS 45.0%
GTTATGAGTGCAGGCAAGAA+GGG - chr3_2:83223237-83223256 Msa0431200:CDS 45.0%
TGAGGATTCAAGCACTTGTC+AGG - chr3_2:83223284-83223303 Msa0431200:CDS 45.0%
GAGGATTCAAGCACTTGTCA+GGG - chr3_2:83223285-83223304 Msa0431200:CDS 45.0%
AGGATTCAAGCACTTGTCAG+GGG - chr3_2:83223286-83223305 Msa0431200:CDS 45.0%
AGCAAGCTGCAGTGACATTA+AGG - chr3_2:83223323-83223342 Msa0431200:CDS 45.0%
AGTGACATTAAGGTGCATGC+AGG - chr3_2:83223333-83223352 Msa0431200:CDS 45.0%
TGCATACTCACTTGCTCAGA+AGG - chr3_2:83223653-83223672 Msa0431200:CDS 45.0%
TAATTGACGACGCCTTACTC+AGG + chr3_2:83223711-83223730 None:intergenic 45.0%
GAGAGTGGAAAATGAGGTGT+TGG + chr3_2:83224024-83224043 None:intergenic 45.0%
! ACACCTCATTTTCCACTCTC+CGG - chr3_2:83224024-83224043 Msa0431200:intron 45.0%
ATTCCGGAGAGTGGAAAATG+AGG + chr3_2:83224030-83224049 None:intergenic 45.0%
TGAACAAAGCTAATGGGGGA+TGG - chr3_2:83224057-83224076 Msa0431200:intron 45.0%
! GATGGAGTTGGTTGGAAAGA+TGG - chr3_2:83224075-83224094 Msa0431200:intron 45.0%
! GAGTTGGTTGGAAAGATGGA+TGG - chr3_2:83224079-83224098 Msa0431200:intron 45.0%
TCCATCAATCTAGTCTCCCA+AGG + chr3_2:83224113-83224132 None:intergenic 45.0%
! GCAAATTTCTTTGGAGGTGG+CGG + chr3_2:83224170-83224189 None:intergenic 45.0%
! CGAGTAGCTTGTCCAATATG+GGG + chr3_2:83224281-83224300 None:intergenic 45.0%
CAAGTCCAAGTTCCGAGTTT+CGG - chr3_2:83224309-83224328 Msa0431200:CDS 45.0%
ACTCTCATCGTACCGAAACT+CGG + chr3_2:83224324-83224343 None:intergenic 45.0%
ACATCCACAACTCCAATGTC+AGG - chr3_2:83224365-83224384 Msa0431200:intron 45.0%
CATCCACAACTCCAATGTCA+GGG - chr3_2:83224366-83224385 Msa0431200:intron 45.0%
TCACAAGTGTTCCCTGACAT+TGG + chr3_2:83224380-83224399 None:intergenic 45.0%
TGTCAGGGAACACTTGTGAA+AGG - chr3_2:83224381-83224400 Msa0431200:intron 45.0%
GAACCTGACTCAATCAACCA+AGG - chr3_2:83224442-83224461 Msa0431200:CDS 45.0%
TTGCCTTGGTTGATTGAGTC+AGG + chr3_2:83224448-83224467 None:intergenic 45.0%
!!! ACCCGATTTTAGCTTTGCCT+TGG + chr3_2:83224462-83224481 None:intergenic 45.0%
AGCTCAAAGGCAACAGTCAA+TGG - chr3_2:83224502-83224521 Msa0431200:CDS 45.0%
ACTTCACCTACCAACCCAAT+CGG - chr3_2:83224619-83224638 Msa0431200:CDS 45.0%
GGTGAGACCATGGTGAAAAA+GGG - chr3_2:83224652-83224671 Msa0431200:intron 45.0%
TGAAAAAGGGAAATAGCGGC+TGG - chr3_2:83224665-83224684 Msa0431200:intron 45.0%
GAAAAAGGGAAATAGCGGCT+GGG - chr3_2:83224666-83224685 Msa0431200:intron 45.0%
CTCAACCTGGAGAGATTATC+TGG - chr3_2:83224871-83224890 Msa0431200:intron 45.0%
TTCGTCCAGATAATCTCTCC+AGG + chr3_2:83224879-83224898 None:intergenic 45.0%
GAAAGAGAGACACTTGTGCA+TGG - chr3_2:83225224-83225243 Msa0431200:intron 45.0%
TGACACCATGCTTGAAGTTG+AGG - chr3_2:83225378-83225397 Msa0431200:CDS 45.0%
GTTTCGGCATTCACCTGATA+TGG + chr3_2:83225435-83225454 None:intergenic 45.0%
TTCTGACCAATGCAAGCTCT+AGG - chr3_2:83225509-83225528 Msa0431200:five_prime_UTR 45.0%
ATTGGTCCTAGAGCTTGCAT+TGG + chr3_2:83225518-83225537 None:intergenic 45.0%
!! GCTCTAGGACCAATTCCAAA+TGG - chr3_2:83225524-83225543 Msa0431200:five_prime_UTR 45.0%
TGTTTGAAGGTGAAGGAGGG+TGG - chr3_2:83222654-83222673 Msa0431200:three_prime_UTR 50.0%
! CTTCAGAAGCTGCTTTGTGG+TGG + chr3_2:83222752-83222771 None:intergenic 50.0%
CACCACAAAGCAGCTTCTGA+AGG - chr3_2:83222750-83222769 Msa0431200:three_prime_UTR 50.0%
! ACCCTTCAGAAGCTGCTTTG+TGG + chr3_2:83222755-83222774 None:intergenic 50.0%
GTGTAAGCTTCTGCAGCAGT+TGG + chr3_2:83222786-83222805 None:intergenic 50.0%
AGAAGCTTACACTGCTGCAG+TGG - chr3_2:83222794-83222813 Msa0431200:three_prime_UTR 50.0%
CGTTGTTAGAGCTCAGCCTA+AGG - chr3_2:83222821-83222840 Msa0431200:three_prime_UTR 50.0%
GGTTATGAGTGCAGGCAAGA+AGG - chr3_2:83223236-83223255 Msa0431200:CDS 50.0%
!! AAGGGCTTTGAGGGCATTGA+AGG - chr3_2:83223255-83223274 Msa0431200:CDS 50.0%
!! TTTGAGGGCATTGAAGGCAG+TGG - chr3_2:83223261-83223280 Msa0431200:CDS 50.0%
TTGTCAGGGGTAGACAAGTG+AGG - chr3_2:83223299-83223318 Msa0431200:CDS 50.0%
TCGTCGTGTGAGGATGTCTA+TGG - chr3_2:83223387-83223406 Msa0431200:CDS 50.0%
TCGTGTGAGGATGTCTATGG+AGG - chr3_2:83223390-83223409 Msa0431200:CDS 50.0%
CGTGTGAGGATGTCTATGGA+GGG - chr3_2:83223391-83223410 Msa0431200:CDS 50.0%
TTGTGAATCTGCAGGAAGGG+TGG - chr3_2:83223547-83223566 Msa0431200:CDS 50.0%
GAAGGGTGGTGTGATAGCAT+AGG - chr3_2:83223561-83223580 Msa0431200:CDS 50.0%
CGCGAATTAGTGCTTGAGGT+TGG + chr3_2:83223999-83224018 None:intergenic 50.0%
GGTTCGCGAATTAGTGCTTG+AGG + chr3_2:83224003-83224022 None:intergenic 50.0%
AAGCTAATGGGGGATGGAGT+TGG - chr3_2:83224063-83224082 Msa0431200:intron 50.0%
! TAATGGGGGATGGAGTTGGT+TGG - chr3_2:83224067-83224086 Msa0431200:intron 50.0%
CAATCTAGTCTCCCAAGGCT+TGG + chr3_2:83224108-83224127 None:intergenic 50.0%
GCCTTGGGAGACTAGATTGA+TGG - chr3_2:83224109-83224128 Msa0431200:intron 50.0%
! ATTTCTTTGGAGGTGGCGGT+GGG + chr3_2:83224166-83224185 None:intergenic 50.0%
! AATTTCTTTGGAGGTGGCGG+TGG + chr3_2:83224167-83224186 None:intergenic 50.0%
AATATGGGGAGGAGGCTTAG+CGG + chr3_2:83224267-83224286 None:intergenic 50.0%
! GTAGCTTGTCCAATATGGGG+AGG + chr3_2:83224278-83224297 None:intergenic 50.0%
TCGTACCGAAACTCGGAACT+TGG + chr3_2:83224317-83224336 None:intergenic 50.0%
GTTCCCTGACATTGGAGTTG+TGG + chr3_2:83224372-83224391 None:intergenic 50.0%
GAACACTTGTGAAAGGACCG+AGG - chr3_2:83224388-83224407 Msa0431200:intron 50.0%
! AGGGCTGCAGTTTTCTCCAA+CGG - chr3_2:83224545-83224564 Msa0431200:CDS 50.0%
!! GATTGGGTTGGTAGGTGAAG+TGG + chr3_2:83224620-83224639 None:intergenic 50.0%
!! TCTTGTCCGATTGGGTTGGT+AGG + chr3_2:83224628-83224647 None:intergenic 50.0%
AACCCAATCGGACAAGAGCT+CGG - chr3_2:83224631-83224650 Msa0431200:intron 50.0%
CGGTGAGACCATGGTGAAAA+AGG - chr3_2:83224651-83224670 Msa0431200:intron 50.0%
GCAACACTCATGGGTGCTTC+TGG - chr3_2:83222486-83222505 Msa0431200:three_prime_UTR 55.0%
! AGAAGGCGCGGAAAGTGGTT+TGG + chr3_2:83222887-83222906 None:intergenic 55.0%
AACCACTTTCCGCGCCTTCT+TGG - chr3_2:83222887-83222906 Msa0431200:three_prime_UTR 55.0%
! TACCAAGAAGGCGCGGAAAG+TGG + chr3_2:83222892-83222911 None:intergenic 55.0%
TGCAGGCAAGAAGGGCTTTG+AGG - chr3_2:83223245-83223264 Msa0431200:CDS 55.0%
! GCAGGCAAGAAGGGCTTTGA+GGG - chr3_2:83223246-83223265 Msa0431200:CDS 55.0%
GAGTAAGAGCTCGTCGTGTG+AGG - chr3_2:83223377-83223396 Msa0431200:CDS 55.0%
!! TTGAGGTTGGCCTGCATTGC+TGG + chr3_2:83223986-83224005 None:intergenic 55.0%
CATCTCGTGATTCCGGAGAG+TGG + chr3_2:83224039-83224058 None:intergenic 55.0%
ATGGATGGCAGCCAAGCCTT+GGG - chr3_2:83224094-83224113 Msa0431200:intron 55.0%
! TTTCTTTGGAGGTGGCGGTG+GGG + chr3_2:83224165-83224184 None:intergenic 55.0%
GCTAAGCCTCCTCCCCATAT+TGG - chr3_2:83224266-83224285 Msa0431200:CDS 55.0%
GCTTGTCCAATATGGGGAGG+AGG + chr3_2:83224275-83224294 None:intergenic 55.0%
GAGCTCTTGTCCGATTGGGT+TGG + chr3_2:83224632-83224651 None:intergenic 55.0%
CACCGAGCTCTTGTCCGATT+GGG + chr3_2:83224636-83224655 None:intergenic 55.0%
TCACCGAGCTCTTGTCCGAT+TGG + chr3_2:83224637-83224656 None:intergenic 55.0%
! ACAAGAGCTCGGTGAGACCA+TGG - chr3_2:83224642-83224661 Msa0431200:intron 55.0%
AAAGGGAAATAGCGGCTGGG+TGG - chr3_2:83224669-83224688 Msa0431200:intron 55.0%
!! AGAAGTGTTCGTGGGGGGTG+TGG + chr3_2:83222333-83222352 Msa0431190:CDS 60.0%
!! GGGCATTGAAGGCAGTGGTG+AGG - chr3_2:83223266-83223285 Msa0431200:CDS 60.0%
GATGGATGGCAGCCAAGCCT+TGG - chr3_2:83224093-83224112 Msa0431200:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 83222324 83225570 83222324 ID=Msa0431200;Name=Msa0431200
chr3_2 mRNA 83222324 83225570 83222324 ID=Msa0431200-mRNA-1;Parent=Msa0431200;Name=Msa0431200-mRNA-1;_AED=0.02;_eAED=0.02;_QI=171|1|1|1|1|1|5|903|445
chr3_2 exon 83222324 83223907 83222324 ID=Msa0431200-mRNA-1:exon:23195;Parent=Msa0431200-mRNA-1
chr3_2 exon 83224220 83224333 83224220 ID=Msa0431200-mRNA-1:exon:23194;Parent=Msa0431200-mRNA-1
chr3_2 exon 83224417 83224644 83224417 ID=Msa0431200-mRNA-1:exon:23193;Parent=Msa0431200-mRNA-1
chr3_2 exon 83224986 83225231 83224986 ID=Msa0431200-mRNA-1:exon:23192;Parent=Msa0431200-mRNA-1
chr3_2 exon 83225331 83225570 83225331 ID=Msa0431200-mRNA-1:exon:23191;Parent=Msa0431200-mRNA-1
chr3_2 five_prime_UTR 83225400 83225570 83225400 ID=Msa0431200-mRNA-1:five_prime_utr;Parent=Msa0431200-mRNA-1
chr3_2 CDS 83225331 83225399 83225331 ID=Msa0431200-mRNA-1:cds;Parent=Msa0431200-mRNA-1
chr3_2 CDS 83224986 83225231 83224986 ID=Msa0431200-mRNA-1:cds;Parent=Msa0431200-mRNA-1
chr3_2 CDS 83224417 83224644 83224417 ID=Msa0431200-mRNA-1:cds;Parent=Msa0431200-mRNA-1
chr3_2 CDS 83224220 83224333 83224220 ID=Msa0431200-mRNA-1:cds;Parent=Msa0431200-mRNA-1
chr3_2 CDS 83223227 83223907 83223227 ID=Msa0431200-mRNA-1:cds;Parent=Msa0431200-mRNA-1
chr3_2 three_prime_UTR 83222324 83223226 83222324 ID=Msa0431200-mRNA-1:three_prime_utr;Parent=Msa0431200-mRNA-1
Gene Sequence

>Msa0431200

ATGGGTGCTTCTGGAAAATGGGTCAAAGCATTAATCGGATTCAAGAAACCAGACAAAGATGAACAACATGTGAAGGAGGGTGGTAAAAGTAAGAAATGGAGGTTATGGAGAAGTTCATCAGGAGATAATAGTTCATGGAAGGGTTTCAAAACAAACCACCACAAAGCAGCTTCTGAAGGGTCTGAATCTCCAACTGCTGCAGAAGCTTACACTGCTGCAGTGGCTACCGTTGTTAGAGCTCAGCCTAAGGATTTTAGACTTGTTAGGCAAGAATGGGCTGCTATAAGAATCCAAACCACTTTCCGCGCCTTCTTGGCAAGAAGGGCTTTGAGGGCATTGAAGGCAGTGGTGAGGATTCAAGCACTTGTCAGGGGTAGACAAGTGAGGAAGCAAGCTGCAGTGACATTAAGGTGCATGCAGGCATTAGTTCGTGTTCAAGCTCGAGTAAGAGCTCGTCGTGTGAGGATGTCTATGGAGGGACAAGCCGTGCAGAATATGCTTAATGAACGACGCAGCAAACTTGAACTTTTGAAGGAAGCAGAGGAAGGGTGGTGTGATAGCATAGGAACATTGGAAGATGTTAAATCAAAAATTCAAATGAGGCAAGAAGGAGCTTTCAAAAGAGAGAGGGCACTTGCATACTCACTTGCTCAGAAGCAATGCAGGCCAACCTCAAGCACTAATTCGCGAACCAACACCTCATTTTCCACTCTCCGGAATCACGAGATGAACAAAGCTAATGGGGGATGGAGTTGGTTGGAAAGATGGATGGCAGCCAAGCCTTGGGAGACTAGATTGATGGAACAGTCTCACGCCGAGAGCTTAGAAAAAACCCCACCGCCACCTCCAAAGAAATTTGCAGAACCCTTTGTAAGCTCGAATTTGAAACCATGCTCGGTCAAAGTCAAAAAGAACAATGTCACAACAAGGATTTCCGCTAAGCCTCCTCCCCATATTGGACAAGCTACTCGTTCATCGTCAAGTCCAAGTTCCGAGTTTCGGTACGATGAGAGTTCTGCATCATCTTCTATTTGTACATCCACAACTCCAATGTCAGGGAACACTTGTGAAAGGACCGAGGATAGTTGTAACAGCACTAGGCCAAATTACATGAACCTGACTCAATCAACCAAGGCAAAGCTAAAATCGGGTAGTAATCAAATGTATAACAGAGCTCAAAGGCAACAGTCAATGGATGAGTTTCAATTTATGAAGAGGGCTGCAGTTTTCTCCAACGGAGATTCTAAAAGCATAGCTGCTTCTGATCATTCACTCAACTTTTCTAGGCCACTTCACCTACCAACCCAATCGGACAAGAGCTCGGTGAGACCATGGTGA
Protein sequence

>Msa0431200

MGASGKWVKALIGFKKPDKDEQHVKEGGKSKKWRLWRSSSGDNSSWKGFKTNHHKAASEGSESPTAAEAYTAAVATVVRAQPKDFRLVRQEWAAIRIQTTFRAFLARRALRALKAVVRIQALVRGRQVRKQAAVTLRCMQALVRVQARVRARRVRMSMEGQAVQNMLNERRSKLELLKEAEEGWCDSIGTLEDVKSKIQMRQEGAFKRERALAYSLAQKQCRPTSSTNSRTNTSFSTLRNHEMNKANGGWSWLERWMAAKPWETRLMEQSHAESLEKTPPPPPKKFAEPFVSSNLKPCSVKVKKNNVTTRISAKPPPHIGQATRSSSSPSSEFRYDESSASSSICTSTTPMSGNTCERTEDSCNSTRPNYMNLTQSTKAKLKSGSNQMYNRAQRQQSMDEFQFMKRAAVFSNGDSKSIAASDHSLNFSRPLHLPTQSDKSSVRPW*