AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0520260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0520260 MtrunA17_Chr3g0135851 98.652 445 6 0 1 445 1 445 0.0 907
Msa0520260 MtrunA17_Chr8g0342551 44.091 440 218 10 1 432 1 420 8.49e-76 243
Msa0520260 MtrunA17_Chr2g0330051 44.548 431 189 13 1 427 1 385 3.84e-73 235
Msa0520260 MtrunA17_Chr4g0025441 47.122 278 124 6 1 268 1 265 4.95e-58 197
Msa0520260 MtrunA17_Chr6g0462191 39.016 305 151 11 89 380 105 387 2.84e-42 154
Msa0520260 MtrunA17_Chr1g0200161 43.503 177 90 4 90 263 140 309 1.35e-37 143
Msa0520260 MtrunA17_Chr8g0346021 44.041 193 80 5 89 265 125 305 1.11e-36 141
Msa0520260 MtrunA17_Chr7g0263731 42.478 226 106 7 50 265 64 275 2.27e-35 136
Msa0520260 MtrunA17_Chr1g0181581 44.503 191 86 6 72 259 103 276 8.30e-35 135
Msa0520260 MtrunA17_Chr2g0283321 35.600 250 122 8 93 332 65 285 1.07e-32 125
Msa0520260 MtrunA17_Chr2g0316401 35.514 214 127 6 58 270 42 245 4.83e-32 124
Msa0520260 MtrunA17_Chr4g0045641 30.114 352 207 12 56 386 112 445 1.77e-30 123
Msa0520260 MtrunA17_Chr4g0020671 31.466 232 131 5 50 276 60 268 1.38e-28 117
Msa0520260 MtrunA17_Chr1g0146931 39.604 202 101 3 86 267 117 317 1.12e-25 109
Msa0520260 MtrunA17_Chr1g0204901 49.524 105 51 1 90 192 135 239 3.63e-24 104
Msa0520260 MtrunA17_Chr7g0273861 29.947 187 108 3 86 268 120 287 2.49e-21 95.9
Msa0520260 MtrunA17_Chr8g0340761 62.162 74 28 0 85 158 120 193 3.75e-21 95.1
Msa0520260 MtrunA17_Chr3g0126901 38.947 190 108 4 91 279 112 294 6.26e-21 94.7
Msa0520260 MtrunA17_Chr8g0371131 40.000 140 74 3 28 159 2 139 9.95e-21 88.2
Msa0520260 MtrunA17_Chr6g0472551 56.098 82 36 0 86 167 112 193 9.17e-20 91.3
Msa0520260 MtrunA17_Chr8g0389781 63.636 66 24 0 90 155 125 190 1.18e-19 90.9
Msa0520260 MtrunA17_Chr2g0330921 36.628 172 89 5 1 159 1 165 1.43e-18 86.7
Msa0520260 MtrunA17_Chr1g0203711 58.571 70 29 0 90 159 114 183 1.55e-18 87.4
Msa0520260 MtrunA17_Chr4g0060381 33.158 190 97 5 74 262 83 243 4.43e-18 86.7
Msa0520260 MtrunA17_Chr4g0057321 33.511 188 99 6 1 180 1 170 1.22e-17 84.7
Msa0520260 MtrunA17_Chr7g0273951 56.716 67 29 0 93 159 108 174 1.60e-17 84.0
Msa0520260 MtrunA17_Chr1g0166431 47.222 108 52 1 90 192 116 223 4.24e-17 83.2
Msa0520260 MtrunA17_Chr5g0416161 36.290 124 63 3 69 191 99 207 5.59e-13 71.2
Msa0520260 MtrunA17_Chr1g0172611 32.847 137 81 2 93 220 107 241 5.96e-13 70.1
Msa0520260 MtrunA17_Chr1g0156121 49.254 67 34 0 91 157 124 190 1.06e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0520260 AT2G26180.1 59.502 442 147 12 1 439 1 413 3.92e-149 431
Msa0520260 AT1G72670.1 45.618 445 189 14 1 436 1 401 1.96e-94 291
Msa0520260 AT1G17480.1 37.277 448 196 14 1 440 1 371 1.66e-65 215
Msa0520260 AT3G22190.3 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa0520260 AT3G22190.2 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa0520260 AT3G22190.4 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa0520260 AT3G22190.1 45.161 279 122 8 1 268 1 259 4.43e-49 173
Msa0520260 AT5G03040.1 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa0520260 AT5G03040.3 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa0520260 AT5G03040.2 43.627 204 105 4 71 272 94 289 4.07e-41 152
Msa0520260 AT4G00820.1 33.668 398 180 15 89 443 130 486 1.42e-37 144
Msa0520260 AT1G01110.2 31.159 414 228 12 14 385 44 442 2.29e-36 140
Msa0520260 AT3G52290.1 39.674 184 102 2 90 273 107 281 3.91e-35 135
Msa0520260 AT3G15050.1 38.496 226 110 8 91 315 50 247 1.02e-32 125
Msa0520260 AT3G09710.1 38.839 224 108 7 64 269 67 279 1.35e-32 129
Msa0520260 AT3G49260.3 40.513 195 93 4 86 265 115 301 1.94e-32 129
Msa0520260 AT3G49260.1 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa0520260 AT3G49260.4 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa0520260 AT3G49260.2 40.000 195 93 4 86 265 115 300 2.20e-32 128
Msa0520260 AT3G09710.2 36.709 237 109 8 64 269 67 293 4.05e-31 125
Msa0520260 AT2G33990.1 37.853 177 99 4 90 265 46 212 1.21e-29 116
Msa0520260 AT4G10640.1 31.864 295 154 9 15 278 22 300 4.70e-27 112
Msa0520260 AT2G26410.2 35.028 177 106 4 89 263 146 315 1.84e-25 109
Msa0520260 AT2G26410.1 35.028 177 106 4 89 263 146 315 1.84e-25 109
Msa0520260 AT5G13460.1 31.897 232 120 8 58 284 86 284 6.25e-23 101
Msa0520260 AT2G43680.4 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa0520260 AT2G43680.2 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa0520260 AT2G43680.3 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa0520260 AT2G43680.5 32.402 179 102 5 77 255 311 470 1.15e-21 98.6
Msa0520260 AT2G43680.1 32.402 179 102 5 77 255 312 471 1.25e-21 98.2
Msa0520260 AT4G14750.1 30.802 237 143 7 1 221 1 232 1.98e-21 95.9
Msa0520260 AT4G14750.3 30.802 237 143 7 1 221 60 291 3.71e-21 95.9
Msa0520260 AT4G14750.2 30.802 237 143 7 1 221 60 291 4.44e-21 95.5
Msa0520260 AT3G59690.2 34.641 153 92 4 81 231 160 306 7.97e-21 95.5
Msa0520260 AT3G59690.1 34.641 153 92 4 81 231 160 306 7.97e-21 95.5
Msa0520260 AT1G51960.1 33.173 208 117 7 1 198 1 196 8.00e-19 87.8
Msa0520260 AT4G23060.1 57.333 75 32 0 91 165 167 241 2.02e-18 87.8
Msa0520260 AT3G16490.1 49.451 91 38 2 90 180 106 188 2.86e-18 86.7
Msa0520260 AT1G18840.1 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa0520260 AT1G18840.3 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa0520260 AT1G18840.2 48.052 77 40 0 88 164 106 182 7.35e-18 86.7
Msa0520260 AT5G07240.1 66.667 63 20 1 90 151 104 166 2.49e-17 84.0
Msa0520260 AT5G07240.2 66.667 63 20 1 90 151 150 212 2.89e-17 84.3
Msa0520260 AT3G51380.1 57.812 64 27 0 89 152 35 98 8.47e-16 73.6
Msa0520260 AT1G14380.6 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa0520260 AT1G14380.5 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa0520260 AT1G14380.2 35.928 167 90 5 1 157 1 160 1.13e-15 79.7
Msa0520260 AT1G14380.7 35.928 167 90 5 1 157 1 160 1.22e-15 79.7
Msa0520260 AT1G74690.1 39.316 117 66 1 62 178 84 195 1.27e-15 79.7
Msa0520260 AT4G29150.1 61.538 65 25 0 89 153 129 193 1.28e-15 79.0
Msa0520260 AT1G14380.1 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa0520260 AT1G14380.3 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa0520260 AT1G14380.4 35.928 167 90 5 1 157 1 160 1.32e-15 79.7
Msa0520260 AT5G03960.3 37.956 137 74 2 93 222 110 242 7.04e-15 76.6
Msa0520260 AT5G03960.2 37.956 137 74 2 93 222 110 242 7.04e-15 76.6
Msa0520260 AT5G03960.1 37.956 137 74 2 93 222 109 241 7.33e-15 76.6
Msa0520260 AT1G01110.1 28.125 288 162 9 134 385 1 279 1.28e-14 75.5
Msa0520260 AT5G62070.1 61.667 60 23 0 86 145 112 171 2.21e-14 75.1
Msa0520260 AT3G49380.1 38.235 136 67 4 19 147 41 166 3.23e-14 74.3
Msa0520260 AT1G19870.1 35.938 128 60 3 47 152 149 276 4.33e-13 72.0
Msa0520260 AT1G19870.2 53.226 62 29 0 91 152 32 93 1.89e-12 69.7
Msa0520260 AT2G02790.2 41.892 74 43 0 84 157 100 173 9.81e-11 64.3
Msa0520260 AT2G02790.3 41.892 74 43 0 84 157 100 173 9.81e-11 64.3

Find 170 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGACGAATTGTCAATTTA+TGG 0.120322 3_4:-72777504 Msa0520260:three_prime_UTR
TCATGGGTGCTTCTGGAAAA+TGG 0.162318 3_4:-72779893 Msa0520260:CDS
TTATTTCTAATAGTAATTTA+TGG 0.163021 3_4:-72777307 Msa0520260:three_prime_UTR
TGTTATACAACATGATTATT+TGG 0.179043 3_4:-72777348 Msa0520260:three_prime_UTR
CAACAAGATGTTGAACTTCT+TGG 0.237700 3_4:+72777671 None:intergenic
AACGAGTAGCTTGTCCAATA+TGG 0.247199 3_4:+72778114 None:intergenic
CGGTGAGACCATGGTGAAAA+AGG 0.254663 3_4:-72777743 Msa0520260:exon
AAGGGTTCTGCAAATTTCTT+TGG 0.258449 3_4:+72778218 None:intergenic
GTTTCGGCATTCACCTGATA+TGG 0.260918 3_4:+72776962 Msa0520250:CDS
TGAGTCAGGTTCATGTAATT+TGG 0.262145 3_4:+72777963 None:intergenic
GCAACACTCATGGGTGCTTC+TGG 0.264318 3_4:-72779900 Msa0520260:exon
TCTAAAGGGCTTTCCATATC+AGG 0.269204 3_4:-72776975 Msa0520260:three_prime_UTR
AACCACTTTCCGCGCCTTCT+TGG 0.292272 3_4:-72779507 Msa0520260:intron
CCATGAGTGTTGCTGAGTTT+TGG 0.299452 3_4:+72779910 None:intergenic
CTTTGTTCATCTCGTGATTC+CGG 0.304293 3_4:+72778351 None:intergenic
CCTTATGCTTTCATACCATT+TGG 0.309982 3_4:+72776855 Msa0520250:CDS
AGCAAGCTGCAGTGACATTA+AGG 0.326388 3_4:-72779071 Msa0520260:CDS
TCTCACCATTAGATGATATA+TGG 0.337909 3_4:-72777215 Msa0520260:three_prime_UTR
CTCAACCTGGAGAGATTATC+TGG 0.341877 3_4:-72777523 Msa0520260:three_prime_UTR
TGGACGAATTGTCAATTTAT+GGG 0.347242 3_4:-72777503 Msa0520260:three_prime_UTR
TGTCTTGTGCATCATAGAAT+TGG 0.353917 3_4:+72777284 None:intergenic
AGCCCTTTAGAACTCGAATA+TGG 0.355019 3_4:+72776987 Msa0520250:CDS
TATACAGAAAATACTTGTTT+CGG 0.356055 3_4:+72776946 Msa0520250:CDS
GCTAAGCCTCCTCCCCATAT+TGG 0.363494 3_4:-72778128 Msa0520260:CDS
AACCAAGGCAAAGCTAAAAT+CGG 0.368576 3_4:-72777937 Msa0520260:CDS
AATTGTTCATTTGTGAGTAT+TGG 0.372492 3_4:-72777551 Msa0520260:three_prime_UTR
TTGCCTTGGTTGATTGAGTC+AGG 0.373865 3_4:+72777949 None:intergenic
GGTGAGATTTATTGATTCAA+TGG 0.373983 3_4:+72777231 None:intergenic
ACATGTTGTTCATCTTTGTC+TGG 0.374417 3_4:+72779843 None:intergenic
AATTTCTTTGGAGGTGGCGG+TGG 0.375706 3_4:+72778230 None:intergenic
GCTTTCATACCATTTGGAAT+TGG 0.378600 3_4:+72776861 Msa0520250:CDS
CCAAATGGTATGAAAGCATA+AGG 0.390674 3_4:-72776855 Msa0520260:three_prime_UTR
CACCGAGCTCTTGTCCGATT+GGG 0.391424 3_4:+72777761 None:intergenic
TGAGGATTCAAGCACTTGTC+AGG 0.392686 3_4:-72779110 Msa0520260:CDS
CACGAGATGAACAAAGCTAA+TGG 0.399444 3_4:-72778344 Msa0520260:CDS
AGAAGGCGCGGAAAGTGGTT+TGG 0.400404 3_4:+72779510 None:intergenic
ACGAGATGAACAAAGCTAAT+GGG 0.406154 3_4:-72778343 Msa0520260:CDS
CAAGTCCAAGTTCCGAGTTT+CGG 0.408556 3_4:-72778085 Msa0520260:CDS
ACCCTTCAGAAGCTGCTTTG+TGG 0.408756 3_4:+72779642 None:intergenic
GCCTTGGGAGACTAGATTGA+TGG 0.409779 3_4:-72778285 Msa0520260:CDS
GATGGAGTTGGTTGGAAAGA+TGG 0.411885 3_4:-72778319 Msa0520260:CDS
TTCCATATTCGAGTTCTAAA+GGG 0.417925 3_4:-72776989 Msa0520260:three_prime_UTR
TGAAAGCTTGATCTCTTGTA+TGG 0.419879 3_4:-72776913 Msa0520260:three_prime_UTR
ATTCCATATTCGAGTTCTAA+AGG 0.422142 3_4:-72776990 Msa0520260:three_prime_UTR
AAAGTAAGAAATGGAGGTTA+TGG 0.428172 3_4:-72779715 Msa0520260:CDS
TCGTACCGAAACTCGGAACT+TGG 0.431873 3_4:+72778080 None:intergenic
TCACCGAGCTCTTGTCCGAT+TGG 0.433938 3_4:+72777760 None:intergenic
CATGGGTGCTTCTGGAAAAT+GGG 0.436014 3_4:-72779892 Msa0520260:CDS
AAATGGGTCAAAGCATTAAT+CGG 0.439295 3_4:-72779876 Msa0520260:CDS
CAAATGAACAATTATACAAA+TGG 0.444321 3_4:+72777561 None:intergenic
AAGGGCTTTGAGGGCATTGA+AGG 0.446119 3_4:-72779139 Msa0520260:CDS
ATTTCTTTGGAGGTGGCGGT+GGG 0.446397 3_4:+72778231 None:intergenic
TCACACCATATATCATCTAA+TGG 0.448162 3_4:+72777210 None:intergenic
ACGAGTAGCTTGTCCAATAT+GGG 0.450987 3_4:+72778115 None:intergenic
TTAGACTTGTTAGGCAAGAA+TGG 0.452196 3_4:-72779547 Msa0520260:CDS
AGCTCAAAGGCAACAGTCAA+TGG 0.457003 3_4:-72777892 Msa0520260:CDS
TGTGAAGTAGAGAGAGAAAG+AGG 0.457973 3_4:+72780021 None:intergenic
TAGACTTGTTAGGCAAGAAT+GGG 0.462512 3_4:-72779546 Msa0520260:CDS
GGTTCTGCAAATTTCTTTGG+AGG 0.465140 3_4:+72778221 None:intergenic
TGCAGGCAAGAAGGGCTTTG+AGG 0.465225 3_4:-72779149 Msa0520260:intron
AGGGTGGTAAAAGTAAGAAA+TGG 0.466097 3_4:-72779724 Msa0520260:CDS
TTTGAGATTTCCAGCAATGC+AGG 0.466329 3_4:-72778421 Msa0520260:intron
GATGGATGGCAGCCAAGCCT+TGG 0.468070 3_4:-72778301 Msa0520260:CDS
CTAACAAGTCTAAAATCCTT+AGG 0.472089 3_4:+72779557 None:intergenic
GCAGGCAAGAAGGGCTTTGA+GGG 0.472599 3_4:-72779148 Msa0520260:intron
TCTGCAAATTTCTTTGGAGG+TGG 0.475452 3_4:+72778224 None:intergenic
TTCAAATTCGAGCTTACAAA+GGG 0.481570 3_4:+72778200 None:intergenic
TCGTCGTGTGAGGATGTCTA+TGG 0.489827 3_4:-72779007 Msa0520260:CDS
CATCAGGAGATAATAGTTCA+TGG 0.490849 3_4:-72779685 Msa0520260:CDS
ACATCCACAACTCCAATGTC+AGG 0.493112 3_4:-72778029 Msa0520260:CDS
GGGAGAGAAAGAAGTGTTCG+TGG 0.495975 3_4:+72780045 None:intergenic
GGTGTGACTTATTCATTCAA+TGG 0.499281 3_4:-72777194 Msa0520260:three_prime_UTR
CTTCAGAAGCTGCTTTGTGG+TGG 0.503461 3_4:+72779645 None:intergenic
CGCGAATTAGTGCTTGAGGT+TGG 0.505381 3_4:+72778398 None:intergenic
TGAGTTTCAATTTATGAAGA+GGG 0.508341 3_4:-72777868 Msa0520260:CDS
GCAAATTTCTTTGGAGGTGG+CGG 0.508534 3_4:+72778227 None:intergenic
AGAGGATGAAATATAAACTT+AGG 0.508574 3_4:+72777113 None:intergenic
TGTTTGAAGGTGAAGGAGGG+TGG 0.510225 3_4:-72779740 Msa0520260:intron
ATGGATGGCAGCCAAGCCTT+GGG 0.511965 3_4:-72778300 Msa0520260:CDS
AGGTTATGGAGAAGTTCATC+AGG 0.515436 3_4:-72779701 Msa0520260:CDS
GTCCTTGATGTTACTTGTGA+AGG 0.518061 3_4:-72777590 Msa0520260:three_prime_UTR
AAGTAGAGAGAGAAAGAGGA+GGG 0.518432 3_4:+72780025 None:intergenic
TGACACCATGCTTGAAGTTG+AGG 0.519275 3_4:-72777016 Msa0520260:three_prime_UTR
AAAATTCAAATGAGGCAAGA+AGG 0.523644 3_4:-72778788 Msa0520260:CDS
TGCATACTCACTTGCTCAGA+AGG 0.524622 3_4:-72778741 Msa0520260:intron
ACCAAGGCAAAGCTAAAATC+GGG 0.526058 3_4:-72777936 Msa0520260:CDS
TCACAAGTGTTCCCTGACAT+TGG 0.528516 3_4:+72778017 None:intergenic
CCAAAACTCAGCAACACTCA+TGG 0.532255 3_4:-72779910 Msa0520260:exon
GGTTATGAGTGCAGGCAAGA+AGG 0.532390 3_4:-72779158 Msa0520260:intron
GTTATGAGTGCAGGCAAGAA+GGG 0.535330 3_4:-72779157 Msa0520260:intron
ACTCTCATCGTACCGAAACT+CGG 0.535615 3_4:+72778073 None:intergenic
TTCATTAAGCATATTCTGCA+CGG 0.537002 3_4:+72778975 None:intergenic
CGTTGTTAGAGCTCAGCCTA+AGG 0.538437 3_4:-72779573 Msa0520260:CDS
TACCAAGAAGGCGCGGAAAG+TGG 0.545045 3_4:+72779505 None:intergenic
ACTTCACCTACCAACCCAAT+CGG 0.545649 3_4:-72777775 Msa0520260:CDS
CAATCTAGTCTCCCAAGGCT+TGG 0.546403 3_4:+72778289 None:intergenic
CACCACAAAGCAGCTTCTGA+AGG 0.550489 3_4:-72779644 Msa0520260:CDS
CAAATGGTATGAAAGCATAA+GGG 0.551950 3_4:-72776854 Msa0520260:three_prime_UTR
GTGTAAGCTTCTGCAGCAGT+TGG 0.555988 3_4:+72779611 None:intergenic
TTCGTCCAGATAATCTCTCC+AGG 0.556575 3_4:+72777518 None:intergenic
TTTGAGGGCATTGAAGGCAG+TGG 0.558218 3_4:-72779133 Msa0520260:CDS
AGGAGATAATAGTTCATGGA+AGG 0.563394 3_4:-72779681 Msa0520260:CDS
GGAGAGAAAGAAGTGTTCGT+GGG 0.565014 3_4:+72780046 None:intergenic
ACTTATACTTTGAGAGTCAG+AGG 0.565119 3_4:+72777095 None:intergenic
GCTCTAGGACCAATTCCAAA+TGG 0.565328 3_4:-72776870 Msa0520260:three_prime_UTR
TCCTTGATGTTACTTGTGAA+GGG 0.565990 3_4:-72777589 Msa0520260:three_prime_UTR
TTATGTTTGAAGGTGAAGGA+GGG 0.568504 3_4:-72779743 Msa0520260:intron
ATGAGTTTCAATTTATGAAG+AGG 0.569955 3_4:-72777869 Msa0520260:CDS
CGAGATGAACAAAGCTAATG+GGG 0.570107 3_4:-72778342 Msa0520260:CDS
GAGAGTGGAAAATGAGGTGT+TGG 0.571769 3_4:+72778373 None:intergenic
CAAAACTCAGCAACACTCAT+GGG 0.571912 3_4:-72779909 Msa0520260:exon
GAAGTAGAGAGAGAAAGAGG+AGG 0.573417 3_4:+72780024 None:intergenic
GAGCTCTTGTCCGATTGGGT+TGG 0.575108 3_4:+72777765 None:intergenic
AGTATTGGTAATACTCAACC+TGG 0.575609 3_4:-72777536 Msa0520260:three_prime_UTR
GAGTAAGAGCTCGTCGTGTG+AGG 0.575741 3_4:-72779017 Msa0520260:CDS
TTTCAAATTCGAGCTTACAA+AGG 0.575937 3_4:+72778199 None:intergenic
GTTCCCTGACATTGGAGTTG+TGG 0.575976 3_4:+72778025 None:intergenic
CTCGAATTTGAAACCATGCT+CGG 0.576259 3_4:-72778189 Msa0520260:CDS
GCTTGTCCAATATGGGGAGG+AGG 0.576470 3_4:+72778122 None:intergenic
TCTTGTCCGATTGGGTTGGT+AGG 0.578767 3_4:+72777769 None:intergenic
GTGGTAAAAGTAAGAAATGG+AGG 0.581415 3_4:-72779721 Msa0520260:CDS
AGTGACATTAAGGTGCATGC+AGG 0.581722 3_4:-72779061 Msa0520260:CDS
AGGATAGTTGTAACAGCACT+AGG 0.582943 3_4:-72777986 Msa0520260:CDS
TCGTGTGAGGATGTCTATGG+AGG 0.584361 3_4:-72779004 Msa0520260:CDS
TTCTGACCAATGCAAGCTCT+AGG 0.584781 3_4:-72776885 Msa0520260:three_prime_UTR
GATTGGGTTGGTAGGTGAAG+TGG 0.587150 3_4:+72777777 None:intergenic
CATCTCGTGATTCCGGAGAG+TGG 0.591885 3_4:+72778358 None:intergenic
GTGTGATAGCATAGGAACAT+TGG 0.592155 3_4:-72778825 Msa0520260:CDS
AATATGGGGAGGAGGCTTAG+CGG 0.592945 3_4:+72778130 None:intergenic
TCCCTTCACAAGTAACATCA+AGG 0.592979 3_4:+72777588 None:intergenic
AACCCAATCGGACAAGAGCT+CGG 0.593120 3_4:-72777763 Msa0520260:CDS
GAGGATTCAAGCACTTGTCA+GGG 0.594722 3_4:-72779109 Msa0520260:CDS
TTTCTTTGGAGGTGGCGGTG+GGG 0.595890 3_4:+72778232 None:intergenic
ATTGGTCCTAGAGCTTGCAT+TGG 0.600573 3_4:+72776879 Msa0520250:CDS
TTTATGTTTGAAGGTGAAGG+AGG 0.601205 3_4:-72779744 Msa0520260:intron
ATGAACAATTATACAAATGG+AGG 0.601507 3_4:+72777564 None:intergenic
ACCACAAAGCAGCTTCTGAA+GGG 0.605684 3_4:-72779643 Msa0520260:CDS
AGGAGCTTTCAAAAGAGAGA+GGG 0.607085 3_4:-72778768 Msa0520260:CDS
GGAGATAATAGTTCATGGAA+GGG 0.611321 3_4:-72779680 Msa0520260:CDS
AGTGCTGTTACAACTATCCT+CGG 0.615531 3_4:+72777989 None:intergenic
GAAGGGTGGTGTGATAGCAT+AGG 0.616172 3_4:-72778833 Msa0520260:CDS
AGAAGCTTACACTGCTGCAG+TGG 0.619115 3_4:-72779600 Msa0520260:CDS
ACAAGAGCTCGGTGAGACCA+TGG 0.619435 3_4:-72777752 Msa0520260:CDS
TCCATCAATCTAGTCTCCCA+AGG 0.620091 3_4:+72778284 None:intergenic
AAGGAGCTTTCAAAAGAGAG+AGG 0.623020 3_4:-72778769 Msa0520260:CDS
GAGAAAGAAGTGTTCGTGGG+GGG 0.624533 3_4:+72780049 None:intergenic
GGGCATTGAAGGCAGTGGTG+AGG 0.626128 3_4:-72779128 Msa0520260:CDS
ATAGTCCTCAACTTCAAGCA+TGG 0.629476 3_4:+72777011 Msa0520250:CDS
ATTCCGGAGAGTGGAAAATG+AGG 0.631071 3_4:+72778367 None:intergenic
CGAGTAGCTTGTCCAATATG+GGG 0.633366 3_4:+72778116 None:intergenic
CGTGTGAGGATGTCTATGGA+GGG 0.633588 3_4:-72779003 Msa0520260:CDS
GAGTTGGTTGGAAAGATGGA+TGG 0.634715 3_4:-72778315 Msa0520260:CDS
TGTCAGGGAACACTTGTGAA+AGG 0.634990 3_4:-72778013 Msa0520260:CDS
GGTTCGCGAATTAGTGCTTG+AGG 0.643363 3_4:+72778394 None:intergenic
AAATGTATAACAGAGCTCAA+AGG 0.643845 3_4:-72777905 Msa0520260:CDS
TTGTGAATCTGCAGGAAGGG+TGG 0.652606 3_4:-72778847 Msa0520260:intron
AAAGGGAAATAGCGGCTGGG+TGG 0.656150 3_4:-72777725 Msa0520260:three_prime_UTR
CATCCACAACTCCAATGTCA+GGG 0.657914 3_4:-72778028 Msa0520260:CDS
GAGAGAAAGAAGTGTTCGTG+GGG 0.659539 3_4:+72780047 None:intergenic
GAAAGAGAGACACTTGTGCA+TGG 0.671235 3_4:-72777170 Msa0520260:three_prime_UTR
CTTAGGCTGAGCTCTAACAA+CGG 0.677421 3_4:+72779574 None:intergenic
AATTGAATACCAAGAAGGCG+CGG 0.680101 3_4:+72779498 None:intergenic
AGGATTCAAGCACTTGTCAG+GGG 0.684889 3_4:-72779108 Msa0520260:CDS
TTGTCAGGGGTAGACAAGTG+AGG 0.688660 3_4:-72779095 Msa0520260:CDS
GTAGCTTGTCCAATATGGGG+AGG 0.689343 3_4:+72778119 None:intergenic
GAACCTGACTCAATCAACCA+AGG 0.696026 3_4:-72777952 Msa0520260:CDS
AAAAGAACAATGTCACAACA+AGG 0.709510 3_4:-72778157 Msa0520260:CDS
AGAGAAAGAAGTGTTCGTGG+GGG 0.721084 3_4:+72780048 None:intergenic
GAGATGAACAAAGCTAATGG+GGG 0.721370 3_4:-72778341 Msa0520260:CDS
GAACACTTGTGAAAGGACCG+AGG 0.744801 3_4:-72778006 Msa0520260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAAAAACTTTTTTTTGT+GGG + chr3_4:72777109-72777128 None:intergenic 10.0%
!!! AAAATAAAAACTTTTTTTTG+TGG + chr3_4:72777110-72777129 None:intergenic 10.0%
!! AAACTAAAAAAACATTAAAT+GGG + chr3_4:72777564-72777583 None:intergenic 10.0%
!! TAAACTAAAAAAACATTAAA+TGG + chr3_4:72777565-72777584 None:intergenic 10.0%
!! TTAAAAAATAACAAATTTGA+GGG + chr3_4:72778248-72778267 None:intergenic 10.0%
!! ATTAAAAAATAACAAATTTG+AGG + chr3_4:72778249-72778268 None:intergenic 10.0%
!!! TTATTTCTAATAGTAATTTA+TGG - chr3_4:72779599-72779618 Msa0520260:CDS 10.0%
!!! AATGTTTTTTTAGTTTAGAT+TGG - chr3_4:72777568-72777587 Msa0520260:three_prime_UTR 15.0%
!! TTAAATCAAAAATTCAAATG+AGG - chr3_4:72778110-72778129 Msa0520260:CDS 15.0%
!! ACAAAATAAAATGAAAAACA+TGG + chr3_4:72779447-72779466 None:intergenic 15.0%
!!! AAGTATAAGTACAATTTTAA+CGG - chr3_4:72779824-72779843 Msa0520260:intron 15.0%
!!! TTATTGTTGTTAACTTTGTT+TGG - chr3_4:72777139-72777158 Msa0520260:three_prime_UTR 20.0%
!! AAAATAAATAGTCATCAAAG+AGG + chr3_4:72777485-72777504 None:intergenic 20.0%
!!! TAGTTTAGATTGGAAAATTT+TGG - chr3_4:72777578-72777597 Msa0520260:three_prime_UTR 20.0%
!! ACTAAATTGAACAATTTGAA+AGG + chr3_4:72778281-72778300 None:intergenic 20.0%
!!! ATAATTTGAAATTTCGTTAG+AGG - chr3_4:72778334-72778353 Msa0520260:CDS 20.0%
!! CTTGGAAAAACAAATAAATA+AGG + chr3_4:72779220-72779239 None:intergenic 20.0%
!! CAAATGAACAATTATACAAA+TGG + chr3_4:72779348-72779367 None:intergenic 20.0%
!! TGTTATACAACATGATTATT+TGG - chr3_4:72779558-72779577 Msa0520260:CDS 20.0%
!! TATACAGAAAATACTTGTTT+CGG + chr3_4:72779963-72779982 None:intergenic 20.0%
!! TTTGTTTGGTTTATGTTTGA+AGG - chr3_4:72777153-72777172 Msa0520260:three_prime_UTR 25.0%
! GAAAAAATTGAATACCAAGA+AGG + chr3_4:72777416-72777435 None:intergenic 25.0%
!! TTTGGACATTCATCTATTTT+AGG - chr3_4:72777596-72777615 Msa0520260:three_prime_UTR 25.0%
!! TTGGACATTCATCTATTTTA+GGG - chr3_4:72777597-72777616 Msa0520260:three_prime_UTR 25.0%
! TAGTGTATTGAACTATTTGT+TGG - chr3_4:72777697-72777716 Msa0520260:three_prime_UTR 25.0%
!!! ATTTTATTTTGTGAATCTGC+AGG - chr3_4:72778051-72778070 Msa0520260:CDS 25.0%
!! GTCTATGAATTTTCAAATCA+TGG - chr3_4:72778311-72778330 Msa0520260:CDS 25.0%
! TATTAGTTCCTAAAGTTGTA+GGG + chr3_4:72778375-72778394 None:intergenic 25.0%
! TTATTAGTTCCTAAAGTTGT+AGG + chr3_4:72778376-72778395 None:intergenic 25.0%
! GTTTATTCATTCAAACATAG+TGG - chr3_4:72778399-72778418 Msa0520260:CDS 25.0%
! GAAAGGAAAGAAAAAGAAAA+AGG + chr3_4:72778444-72778463 None:intergenic 25.0%
! ATGAGTTTCAATTTATGAAG+AGG - chr3_4:72779037-72779056 Msa0520260:CDS 25.0%
! TGAGTTTCAATTTATGAAGA+GGG - chr3_4:72779038-72779057 Msa0520260:CDS 25.0%
! ATGAACAATTATACAAATGG+AGG + chr3_4:72779345-72779364 None:intergenic 25.0%
! AATTGTTCATTTGTGAGTAT+TGG - chr3_4:72779355-72779374 Msa0520260:intron 25.0%
!!! TTTTATTTTGTGAGTGTTCA+TGG - chr3_4:72779456-72779475 Msa0520260:intron 25.0%
! AGAGGATGAAATATAAACTT+AGG + chr3_4:72779796-72779815 None:intergenic 25.0%
! AAATGGGTCAAAGCATTAAT+CGG - chr3_4:72777030-72777049 Msa0520260:three_prime_UTR 30.0%
AAAGTAAGAAATGGAGGTTA+TGG - chr3_4:72777191-72777210 Msa0520260:three_prime_UTR 30.0%
! CTAACAAGTCTAAAATCCTT+AGG + chr3_4:72777352-72777371 None:intergenic 30.0%
!! CTAAGGATTTTAGACTTGTT+AGG - chr3_4:72777350-72777369 Msa0520260:three_prime_UTR 30.0%
!! AAAACAAAAGCTTGAGTTGA+AGG + chr3_4:72777441-72777460 None:intergenic 30.0%
! GTATTGAACTATTTGTTGGA+AGG - chr3_4:72777701-72777720 Msa0520260:three_prime_UTR 30.0%
TGTGTTTGTAATGAATGTGA+GGG - chr3_4:72777727-72777746 Msa0520260:three_prime_UTR 30.0%
TTCATTAAGCATATTCTGCA+CGG + chr3_4:72777934-72777953 None:intergenic 30.0%
AAAATTCAAATGAGGCAAGA+AGG - chr3_4:72778118-72778137 Msa0520260:CDS 30.0%
AGGAAAGAAAAAGAAAAAGG+TGG + chr3_4:72778441-72778460 None:intergenic 30.0%
AAGCATCTTTCTTTGTAGAA+AGG + chr3_4:72778461-72778480 None:intergenic 30.0%
TTCAAATTCGAGCTTACAAA+GGG + chr3_4:72778709-72778728 None:intergenic 30.0%
TTTCAAATTCGAGCTTACAA+AGG + chr3_4:72778710-72778729 None:intergenic 30.0%
AAAAGAACAATGTCACAACA+AGG - chr3_4:72778749-72778768 Msa0520260:CDS 30.0%
AAATGTATAACAGAGCTCAA+AGG - chr3_4:72779001-72779020 Msa0520260:CDS 30.0%
! ATCATTCACTCAACTTTTCT+AGG - chr3_4:72779106-72779125 Msa0520260:CDS 30.0%
TGGACGAATTGTCAATTTAT+GGG - chr3_4:72779403-72779422 Msa0520260:intron 30.0%
TTGATTCAATGGCAAAAACT+AGG + chr3_4:72779667-72779686 None:intergenic 30.0%
!! GGTGAGATTTATTGATTCAA+TGG + chr3_4:72779678-72779697 None:intergenic 30.0%
TCTCACCATTAGATGATATA+TGG - chr3_4:72779691-72779710 Msa0520260:CDS 30.0%
TCACACCATATATCATCTAA+TGG + chr3_4:72779699-72779718 None:intergenic 30.0%
! ATTCCATATTCGAGTTCTAA+AGG - chr3_4:72779916-72779935 Msa0520260:five_prime_UTR 30.0%
!! TTCCATATTCGAGTTCTAAA+GGG - chr3_4:72779917-72779936 Msa0520260:five_prime_UTR 30.0%
! CAAATGGTATGAAAGCATAA+GGG - chr3_4:72780052-72780071 Msa0520260:five_prime_UTR 30.0%
ACATGTTGTTCATCTTTGTC+TGG + chr3_4:72777066-72777085 None:intergenic 35.0%
TGGTTTATGTTTGAAGGTGA+AGG - chr3_4:72777159-72777178 Msa0520260:three_prime_UTR 35.0%
TTTATGTTTGAAGGTGAAGG+AGG - chr3_4:72777162-72777181 Msa0520260:three_prime_UTR 35.0%
TTATGTTTGAAGGTGAAGGA+GGG - chr3_4:72777163-72777182 Msa0520260:three_prime_UTR 35.0%
AGGGTGGTAAAAGTAAGAAA+TGG - chr3_4:72777182-72777201 Msa0520260:three_prime_UTR 35.0%
GTGGTAAAAGTAAGAAATGG+AGG - chr3_4:72777185-72777204 Msa0520260:three_prime_UTR 35.0%
CATCAGGAGATAATAGTTCA+TGG - chr3_4:72777221-72777240 Msa0520260:three_prime_UTR 35.0%
AGGAGATAATAGTTCATGGA+AGG - chr3_4:72777225-72777244 Msa0520260:three_prime_UTR 35.0%
GGAGATAATAGTTCATGGAA+GGG - chr3_4:72777226-72777245 Msa0520260:three_prime_UTR 35.0%
!! TTAGACTTGTTAGGCAAGAA+TGG - chr3_4:72777359-72777378 Msa0520260:three_prime_UTR 35.0%
!! TAGACTTGTTAGGCAAGAAT+GGG - chr3_4:72777360-72777379 Msa0520260:three_prime_UTR 35.0%
CTGTGTTTGTAATGAATGTG+AGG - chr3_4:72777726-72777745 Msa0520260:three_prime_UTR 35.0%
! CAGCAAACTTGAACTTTTGA+AGG - chr3_4:72777959-72777978 Msa0520260:CDS 35.0%
TATGTGTTGTGAATTGACAG+TGG - chr3_4:72777991-72778010 Msa0520260:CDS 35.0%
! TATTTTGTGAATCTGCAGGA+AGG - chr3_4:72778055-72778074 Msa0520260:CDS 35.0%
! ATTTTGTGAATCTGCAGGAA+GGG - chr3_4:72778056-72778075 Msa0520260:CDS 35.0%
! TTAAGTTGCCCTACAACTTT+AGG - chr3_4:72778364-72778383 Msa0520260:CDS 35.0%
ACGAGATGAACAAAGCTAAT+GGG - chr3_4:72778563-72778582 Msa0520260:intron 35.0%
AAGGGTTCTGCAAATTTCTT+TGG + chr3_4:72778691-72778710 None:intergenic 35.0%
TGAGTCAGGTTCATGTAATT+TGG + chr3_4:72778946-72778965 None:intergenic 35.0%
AACCAAGGCAAAGCTAAAAT+CGG - chr3_4:72778969-72778988 Msa0520260:CDS 35.0%
!!! TATGCTTTTAGAATCTCCGT+TGG + chr3_4:72779076-72779095 None:intergenic 35.0%
ATGGTGAAAAAGGGAAATAG+CGG - chr3_4:72779173-72779192 Msa0520260:intron 35.0%
CAACAAGATGTTGAACTTCT+TGG + chr3_4:72779238-72779257 None:intergenic 35.0%
! TCCTTGATGTTACTTGTGAA+GGG - chr3_4:72779317-72779336 Msa0520260:intron 35.0%
!! AGTATTGGTAATACTCAACC+TGG - chr3_4:72779370-72779389 Msa0520260:intron 35.0%
CTGGACGAATTGTCAATTTA+TGG - chr3_4:72779402-72779421 Msa0520260:intron 35.0%
TGTCTTGTGCATCATAGAAT+TGG + chr3_4:72779625-72779644 None:intergenic 35.0%
GGTGTGACTTATTCATTCAA+TGG - chr3_4:72779712-72779731 Msa0520260:CDS 35.0%
! ACTTATACTTTGAGAGTCAG+AGG + chr3_4:72779814-72779833 None:intergenic 35.0%
TGAAAGCTTGATCTCTTGTA+TGG - chr3_4:72779993-72780012 Msa0520260:five_prime_UTR 35.0%
! GCTTTCATACCATTTGGAAT+TGG + chr3_4:72780048-72780067 None:intergenic 35.0%
CCTTATGCTTTCATACCATT+TGG + chr3_4:72780054-72780073 None:intergenic 35.0%
! CCAAATGGTATGAAAGCATA+AGG - chr3_4:72780051-72780070 Msa0520260:five_prime_UTR 35.0%
AAGTAGAGAGAGAAAGAGGA+GGG + chr3_4:72776884-72776903 Msa0520250:CDS 40.0%
TGTGAAGTAGAGAGAGAAAG+AGG + chr3_4:72776888-72776907 Msa0520250:CDS 40.0%
CAAAACTCAGCAACACTCAT+GGG - chr3_4:72776997-72777016 Msa0520260:three_prime_UTR 40.0%
AGGTTATGGAGAAGTTCATC+AGG - chr3_4:72777205-72777224 Msa0520260:three_prime_UTR 40.0%
AATTGAATACCAAGAAGGCG+CGG + chr3_4:72777411-72777430 None:intergenic 40.0%
!! TGAACTTTTGAAGGAAGCAG+AGG - chr3_4:72777968-72777987 Msa0520260:CDS 40.0%
GTGTGATAGCATAGGAACAT+TGG - chr3_4:72778081-72778100 Msa0520260:CDS 40.0%
AAGGAGCTTTCAAAAGAGAG+AGG - chr3_4:72778137-72778156 Msa0520260:CDS 40.0%
AGGAGCTTTCAAAAGAGAGA+GGG - chr3_4:72778138-72778157 Msa0520260:CDS 40.0%
! TCTCTTTTCAGTCCTGAGTA+AGG - chr3_4:72778208-72778227 Msa0520260:CDS 40.0%
! TTTGAGATTTCCAGCAATGC+AGG - chr3_4:72778485-72778504 Msa0520260:intron 40.0%
CTTTGTTCATCTCGTGATTC+CGG + chr3_4:72778558-72778577 None:intergenic 40.0%
CACGAGATGAACAAAGCTAA+TGG - chr3_4:72778562-72778581 Msa0520260:intron 40.0%
CGAGATGAACAAAGCTAATG+GGG - chr3_4:72778564-72778583 Msa0520260:intron 40.0%
GAGATGAACAAAGCTAATGG+GGG - chr3_4:72778565-72778584 Msa0520260:intron 40.0%
!!! TGGGGTTTTTTCTAAGCTCT+CGG + chr3_4:72778659-72778678 None:intergenic 40.0%
! TCTGCAAATTTCTTTGGAGG+TGG + chr3_4:72778685-72778704 None:intergenic 40.0%
GGTTCTGCAAATTTCTTTGG+AGG + chr3_4:72778688-72778707 None:intergenic 40.0%
! CTCGAATTTGAAACCATGCT+CGG - chr3_4:72778717-72778736 Msa0520260:intron 40.0%
!!! TTTTTGACTTTGACCGAGCA+TGG + chr3_4:72778733-72778752 None:intergenic 40.0%
! ACGAGTAGCTTGTCCAATAT+GGG + chr3_4:72778794-72778813 None:intergenic 40.0%
! AACGAGTAGCTTGTCCAATA+TGG + chr3_4:72778795-72778814 None:intergenic 40.0%
! AGTGCTGTTACAACTATCCT+CGG + chr3_4:72778920-72778939 None:intergenic 40.0%
AGGATAGTTGTAACAGCACT+AGG - chr3_4:72778920-72778939 Msa0520260:intron 40.0%
ACCAAGGCAAAGCTAAAATC+GGG - chr3_4:72778970-72778989 Msa0520260:CDS 40.0%
!! GCTATTTCCCTTTTTCACCA+TGG + chr3_4:72779174-72779193 None:intergenic 40.0%
! GTCCTTGATGTTACTTGTGA+AGG - chr3_4:72779316-72779335 Msa0520260:intron 40.0%
TCCCTTCACAAGTAACATCA+AGG + chr3_4:72779321-72779340 None:intergenic 40.0%
!!! GTGAGTGTTCATGGTTTTGT+AGG - chr3_4:72779465-72779484 Msa0520260:intron 40.0%
ATAGTCCTCAACTTCAAGCA+TGG + chr3_4:72779898-72779917 None:intergenic 40.0%
!! AGCCCTTTAGAACTCGAATA+TGG + chr3_4:72779922-72779941 None:intergenic 40.0%
TCTAAAGGGCTTTCCATATC+AGG - chr3_4:72779931-72779950 Msa0520260:five_prime_UTR 40.0%
!! AGAGAAAGAAGTGTTCGTGG+GGG + chr3_4:72776861-72776880 Msa0520250:CDS 45.0%
!! GAGAGAAAGAAGTGTTCGTG+GGG + chr3_4:72776862-72776881 Msa0520250:CDS 45.0%
!! GGAGAGAAAGAAGTGTTCGT+GGG + chr3_4:72776863-72776882 Msa0520250:CDS 45.0%
GAAGTAGAGAGAGAAAGAGG+AGG + chr3_4:72776885-72776904 Msa0520250:CDS 45.0%
! AGAGAGAGAGAGTGTGAATG+TGG + chr3_4:72776965-72776984 Msa0520250:CDS 45.0%
!! CCATGAGTGTTGCTGAGTTT+TGG + chr3_4:72776999-72777018 Msa0520250:CDS 45.0%
CCAAAACTCAGCAACACTCA+TGG - chr3_4:72776996-72777015 Msa0520260:three_prime_UTR 45.0%
! TCATGGGTGCTTCTGGAAAA+TGG - chr3_4:72777013-72777032 Msa0520260:three_prime_UTR 45.0%
! CATGGGTGCTTCTGGAAAAT+GGG - chr3_4:72777014-72777033 Msa0520260:three_prime_UTR 45.0%
ACCACAAAGCAGCTTCTGAA+GGG - chr3_4:72777263-72777282 Msa0520260:three_prime_UTR 45.0%
! CTTAGGCTGAGCTCTAACAA+CGG + chr3_4:72777335-72777354 None:intergenic 45.0%
AATGTGAGGGTTATGAGTGC+AGG - chr3_4:72777740-72777759 Msa0520260:exon 45.0%
GTTATGAGTGCAGGCAAGAA+GGG - chr3_4:72777749-72777768 Msa0520260:CDS 45.0%
TGAGGATTCAAGCACTTGTC+AGG - chr3_4:72777796-72777815 Msa0520260:CDS 45.0%
GAGGATTCAAGCACTTGTCA+GGG - chr3_4:72777797-72777816 Msa0520260:CDS 45.0%
AGGATTCAAGCACTTGTCAG+GGG - chr3_4:72777798-72777817 Msa0520260:CDS 45.0%
AGCAAGCTGCAGTGACATTA+AGG - chr3_4:72777835-72777854 Msa0520260:CDS 45.0%
AGTGACATTAAGGTGCATGC+AGG - chr3_4:72777845-72777864 Msa0520260:CDS 45.0%
TGCATACTCACTTGCTCAGA+AGG - chr3_4:72778165-72778184 Msa0520260:CDS 45.0%
TAATTGACGACGCCTTACTC+AGG + chr3_4:72778223-72778242 None:intergenic 45.0%
GAGAGTGGAAAATGAGGTGT+TGG + chr3_4:72778536-72778555 None:intergenic 45.0%
! ACACCTCATTTTCCACTCTC+CGG - chr3_4:72778536-72778555 Msa0520260:intron 45.0%
ATTCCGGAGAGTGGAAAATG+AGG + chr3_4:72778542-72778561 None:intergenic 45.0%
TGAACAAAGCTAATGGGGGA+TGG - chr3_4:72778569-72778588 Msa0520260:intron 45.0%
! GATGGAGTTGGTTGGAAAGA+TGG - chr3_4:72778587-72778606 Msa0520260:intron 45.0%
! GAGTTGGTTGGAAAGATGGA+TGG - chr3_4:72778591-72778610 Msa0520260:intron 45.0%
TCCATCAATCTAGTCTCCCA+AGG + chr3_4:72778625-72778644 None:intergenic 45.0%
! GCAAATTTCTTTGGAGGTGG+CGG + chr3_4:72778682-72778701 None:intergenic 45.0%
! CGAGTAGCTTGTCCAATATG+GGG + chr3_4:72778793-72778812 None:intergenic 45.0%
CAAGTCCAAGTTCCGAGTTT+CGG - chr3_4:72778821-72778840 Msa0520260:CDS 45.0%
ACTCTCATCGTACCGAAACT+CGG + chr3_4:72778836-72778855 None:intergenic 45.0%
ACATCCACAACTCCAATGTC+AGG - chr3_4:72778877-72778896 Msa0520260:intron 45.0%
CATCCACAACTCCAATGTCA+GGG - chr3_4:72778878-72778897 Msa0520260:intron 45.0%
TCACAAGTGTTCCCTGACAT+TGG + chr3_4:72778892-72778911 None:intergenic 45.0%
TGTCAGGGAACACTTGTGAA+AGG - chr3_4:72778893-72778912 Msa0520260:intron 45.0%
GAACCTGACTCAATCAACCA+AGG - chr3_4:72778954-72778973 Msa0520260:CDS 45.0%
TTGCCTTGGTTGATTGAGTC+AGG + chr3_4:72778960-72778979 None:intergenic 45.0%
!!! ACCCGATTTTAGCTTTGCCT+TGG + chr3_4:72778974-72778993 None:intergenic 45.0%
AGCTCAAAGGCAACAGTCAA+TGG - chr3_4:72779014-72779033 Msa0520260:CDS 45.0%
ACTTCACCTACCAACCCAAT+CGG - chr3_4:72779131-72779150 Msa0520260:CDS 45.0%
GGTGAGACCATGGTGAAAAA+GGG - chr3_4:72779164-72779183 Msa0520260:intron 45.0%
TGAAAAAGGGAAATAGCGGC+TGG - chr3_4:72779177-72779196 Msa0520260:intron 45.0%
GAAAAAGGGAAATAGCGGCT+GGG - chr3_4:72779178-72779197 Msa0520260:intron 45.0%
CTCAACCTGGAGAGATTATC+TGG - chr3_4:72779383-72779402 Msa0520260:intron 45.0%
TTCGTCCAGATAATCTCTCC+AGG + chr3_4:72779391-72779410 None:intergenic 45.0%
GAAAGAGAGACACTTGTGCA+TGG - chr3_4:72779736-72779755 Msa0520260:intron 45.0%
TGACACCATGCTTGAAGTTG+AGG - chr3_4:72779890-72779909 Msa0520260:CDS 45.0%
GTTTCGGCATTCACCTGATA+TGG + chr3_4:72779947-72779966 None:intergenic 45.0%
TTCTGACCAATGCAAGCTCT+AGG - chr3_4:72780021-72780040 Msa0520260:five_prime_UTR 45.0%
ATTGGTCCTAGAGCTTGCAT+TGG + chr3_4:72780030-72780049 None:intergenic 45.0%
!! GCTCTAGGACCAATTCCAAA+TGG - chr3_4:72780036-72780055 Msa0520260:five_prime_UTR 45.0%
!! GAGAAAGAAGTGTTCGTGGG+GGG + chr3_4:72776860-72776879 Msa0520250:CDS 50.0%
!! GGGAGAGAAAGAAGTGTTCG+TGG + chr3_4:72776864-72776883 Msa0520250:CDS 50.0%
TGTTTGAAGGTGAAGGAGGG+TGG - chr3_4:72777166-72777185 Msa0520260:three_prime_UTR 50.0%
! CTTCAGAAGCTGCTTTGTGG+TGG + chr3_4:72777264-72777283 None:intergenic 50.0%
CACCACAAAGCAGCTTCTGA+AGG - chr3_4:72777262-72777281 Msa0520260:three_prime_UTR 50.0%
! ACCCTTCAGAAGCTGCTTTG+TGG + chr3_4:72777267-72777286 None:intergenic 50.0%
GTGTAAGCTTCTGCAGCAGT+TGG + chr3_4:72777298-72777317 None:intergenic 50.0%
AGAAGCTTACACTGCTGCAG+TGG - chr3_4:72777306-72777325 Msa0520260:three_prime_UTR 50.0%
CGTTGTTAGAGCTCAGCCTA+AGG - chr3_4:72777333-72777352 Msa0520260:three_prime_UTR 50.0%
GGTTATGAGTGCAGGCAAGA+AGG - chr3_4:72777748-72777767 Msa0520260:exon 50.0%
!! AAGGGCTTTGAGGGCATTGA+AGG - chr3_4:72777767-72777786 Msa0520260:CDS 50.0%
!! TTTGAGGGCATTGAAGGCAG+TGG - chr3_4:72777773-72777792 Msa0520260:CDS 50.0%
TTGTCAGGGGTAGACAAGTG+AGG - chr3_4:72777811-72777830 Msa0520260:CDS 50.0%
TCGTCGTGTGAGGATGTCTA+TGG - chr3_4:72777899-72777918 Msa0520260:CDS 50.0%
TCGTGTGAGGATGTCTATGG+AGG - chr3_4:72777902-72777921 Msa0520260:CDS 50.0%
CGTGTGAGGATGTCTATGGA+GGG - chr3_4:72777903-72777922 Msa0520260:CDS 50.0%
TTGTGAATCTGCAGGAAGGG+TGG - chr3_4:72778059-72778078 Msa0520260:CDS 50.0%
GAAGGGTGGTGTGATAGCAT+AGG - chr3_4:72778073-72778092 Msa0520260:CDS 50.0%
CGCGAATTAGTGCTTGAGGT+TGG + chr3_4:72778511-72778530 None:intergenic 50.0%
GGTTCGCGAATTAGTGCTTG+AGG + chr3_4:72778515-72778534 None:intergenic 50.0%
AAGCTAATGGGGGATGGAGT+TGG - chr3_4:72778575-72778594 Msa0520260:intron 50.0%
! TAATGGGGGATGGAGTTGGT+TGG - chr3_4:72778579-72778598 Msa0520260:intron 50.0%
CAATCTAGTCTCCCAAGGCT+TGG + chr3_4:72778620-72778639 None:intergenic 50.0%
GCCTTGGGAGACTAGATTGA+TGG - chr3_4:72778621-72778640 Msa0520260:intron 50.0%
! ATTTCTTTGGAGGTGGCGGT+GGG + chr3_4:72778678-72778697 None:intergenic 50.0%
! AATTTCTTTGGAGGTGGCGG+TGG + chr3_4:72778679-72778698 None:intergenic 50.0%
AATATGGGGAGGAGGCTTAG+CGG + chr3_4:72778779-72778798 None:intergenic 50.0%
! GTAGCTTGTCCAATATGGGG+AGG + chr3_4:72778790-72778809 None:intergenic 50.0%
TCGTACCGAAACTCGGAACT+TGG + chr3_4:72778829-72778848 None:intergenic 50.0%
GTTCCCTGACATTGGAGTTG+TGG + chr3_4:72778884-72778903 None:intergenic 50.0%
GAACACTTGTGAAAGGACCG+AGG - chr3_4:72778900-72778919 Msa0520260:intron 50.0%
! AGGGCTGCAGTTTTCTCCAA+CGG - chr3_4:72779057-72779076 Msa0520260:CDS 50.0%
!! GATTGGGTTGGTAGGTGAAG+TGG + chr3_4:72779132-72779151 None:intergenic 50.0%
!! TCTTGTCCGATTGGGTTGGT+AGG + chr3_4:72779140-72779159 None:intergenic 50.0%
AACCCAATCGGACAAGAGCT+CGG - chr3_4:72779143-72779162 Msa0520260:CDS 50.0%
CGGTGAGACCATGGTGAAAA+AGG - chr3_4:72779163-72779182 Msa0520260:intron 50.0%
GCAACACTCATGGGTGCTTC+TGG - chr3_4:72777006-72777025 Msa0520260:three_prime_UTR 55.0%
! AGAAGGCGCGGAAAGTGGTT+TGG + chr3_4:72777399-72777418 None:intergenic 55.0%
AACCACTTTCCGCGCCTTCT+TGG - chr3_4:72777399-72777418 Msa0520260:three_prime_UTR 55.0%
! TACCAAGAAGGCGCGGAAAG+TGG + chr3_4:72777404-72777423 None:intergenic 55.0%
TGCAGGCAAGAAGGGCTTTG+AGG - chr3_4:72777757-72777776 Msa0520260:CDS 55.0%
! GCAGGCAAGAAGGGCTTTGA+GGG - chr3_4:72777758-72777777 Msa0520260:CDS 55.0%
GAGTAAGAGCTCGTCGTGTG+AGG - chr3_4:72777889-72777908 Msa0520260:CDS 55.0%
!! TTGAGGTTGGCCTGCATTGC+TGG + chr3_4:72778498-72778517 None:intergenic 55.0%
CATCTCGTGATTCCGGAGAG+TGG + chr3_4:72778551-72778570 None:intergenic 55.0%
ATGGATGGCAGCCAAGCCTT+GGG - chr3_4:72778606-72778625 Msa0520260:intron 55.0%
! TTTCTTTGGAGGTGGCGGTG+GGG + chr3_4:72778677-72778696 None:intergenic 55.0%
GCTAAGCCTCCTCCCCATAT+TGG - chr3_4:72778778-72778797 Msa0520260:CDS 55.0%
GCTTGTCCAATATGGGGAGG+AGG + chr3_4:72778787-72778806 None:intergenic 55.0%
GAGCTCTTGTCCGATTGGGT+TGG + chr3_4:72779144-72779163 None:intergenic 55.0%
CACCGAGCTCTTGTCCGATT+GGG + chr3_4:72779148-72779167 None:intergenic 55.0%
TCACCGAGCTCTTGTCCGAT+TGG + chr3_4:72779149-72779168 None:intergenic 55.0%
! ACAAGAGCTCGGTGAGACCA+TGG - chr3_4:72779154-72779173 Msa0520260:intron 55.0%
AAAGGGAAATAGCGGCTGGG+TGG - chr3_4:72779181-72779200 Msa0520260:intron 55.0%
!! GGGCATTGAAGGCAGTGGTG+AGG - chr3_4:72777778-72777797 Msa0520260:CDS 60.0%
GATGGATGGCAGCCAAGCCT+TGG - chr3_4:72778605-72778624 Msa0520260:intron 60.0%
!! AGAAGTGTTCGTGGGGGGCG+TGG + chr3_4:72776855-72776874 Msa0520250:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 72776846 72780082 72776846 ID=Msa0520260;Name=Msa0520260
chr3_4 mRNA 72776846 72780082 72776846 ID=Msa0520260-mRNA-1;Parent=Msa0520260;Name=Msa0520260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=169|1|1|1|1|1|5|903|445
chr3_4 exon 72776846 72778429 72776846 ID=Msa0520260-mRNA-1:exon:20161;Parent=Msa0520260-mRNA-1
chr3_4 exon 72778742 72778855 72778742 ID=Msa0520260-mRNA-1:exon:20160;Parent=Msa0520260-mRNA-1
chr3_4 exon 72778939 72779166 72778939 ID=Msa0520260-mRNA-1:exon:20159;Parent=Msa0520260-mRNA-1
chr3_4 exon 72779508 72779753 72779508 ID=Msa0520260-mRNA-1:exon:20158;Parent=Msa0520260-mRNA-1
chr3_4 exon 72779845 72780082 72779845 ID=Msa0520260-mRNA-1:exon:20157;Parent=Msa0520260-mRNA-1
chr3_4 five_prime_UTR 72779914 72780082 72779914 ID=Msa0520260-mRNA-1:five_prime_utr;Parent=Msa0520260-mRNA-1
chr3_4 CDS 72779845 72779913 72779845 ID=Msa0520260-mRNA-1:cds;Parent=Msa0520260-mRNA-1
chr3_4 CDS 72779508 72779753 72779508 ID=Msa0520260-mRNA-1:cds;Parent=Msa0520260-mRNA-1
chr3_4 CDS 72778939 72779166 72778939 ID=Msa0520260-mRNA-1:cds;Parent=Msa0520260-mRNA-1
chr3_4 CDS 72778742 72778855 72778742 ID=Msa0520260-mRNA-1:cds;Parent=Msa0520260-mRNA-1
chr3_4 CDS 72777749 72778429 72777749 ID=Msa0520260-mRNA-1:cds;Parent=Msa0520260-mRNA-1
chr3_4 three_prime_UTR 72776846 72777748 72776846 ID=Msa0520260-mRNA-1:three_prime_utr;Parent=Msa0520260-mRNA-1
Gene Sequence

>Msa0520260

ATGGGTGCTTCTGGAAAATGGGTCAAAGCATTAATCGGATTCAAGAAACCAGACAAAGATGAACAACATGTGAAGGAGGGTGGTAAAAGTAAGAAATGGAGGTTATGGAGAAGTTCATCAGGAGATAATAGTTCATGGAAGGGTTTCAAAACAAACCACCACAAAGCAGCTTCTGAAGGGTCTGAATCTCCAACTGCTGCAGAAGCTTACACTGCTGCAGTGGCTACCGTTGTTAGAGCTCAGCCTAAGGATTTTAGACTTGTTAGGCAAGAATGGGCTGCTATAAGAATCCAAACCACTTTCCGCGCCTTCTTGGCAAGAAGGGCTTTGAGGGCATTGAAGGCAGTGGTGAGGATTCAAGCACTTGTCAGGGGTAGACAAGTGAGGAAGCAAGCTGCAGTGACATTAAGGTGCATGCAGGCATTAGTTCGTGTTCAAGCTCGAGTAAGAGCTCGTCGTGTGAGGATGTCTATGGAGGGACAAGCCGTGCAGAATATGCTTAATGAACGACGCAGCAAACTTGAACTTTTGAAGGAAGCAGAGGAAGGGTGGTGTGATAGCATAGGAACATTGGAAGATGTTAAATCAAAAATTCAAATGAGGCAAGAAGGAGCTTTCAAAAGAGAGAGGGCACTTGCATACTCACTTGCTCAGAAGCAATGCAGGCCAACCTCAAGCACTAATTCGCGAACCAACACCTCATTTTCCACTCTCCGGAATCACGAGATGAACAAAGCTAATGGGGGATGGAGTTGGTTGGAAAGATGGATGGCAGCCAAGCCTTGGGAGACTAGATTGATGGAACAGTCTCACGCCGAGAGCTTAGAAAAAACCCCACCGCCACCTCCAAAGAAATTTGCAGAACCCTTTGTAAGCTCGAATTTGAAACCATGCTCGGTCAAAGTCAAAAAGAACAATGTCACAACAAGGATTTCCGCTAAGCCTCCTCCCCATATTGGACAAGCTACTCGTTCATCGTCAAGTCCAAGTTCCGAGTTTCGGTACGATGAGAGTTCTGCATCATCTTCTATTTGTACATCCACAACTCCAATGTCAGGGAACACTTGTGAAAGGACCGAGGATAGTTGTAACAGCACTAGGCCAAATTACATGAACCTGACTCAATCAACCAAGGCAAAGCTAAAATCGGGTAGTAATCAAATGTATAACAGAGCTCAAAGGCAACAGTCAATGGATGAGTTTCAATTTATGAAGAGGGCTGCAGTTTTCTCCAACGGAGATTCTAAAAGCATAGCTGCTTCTGATCATTCACTCAACTTTTCTAGGCCACTTCACCTACCAACCCAATCGGACAAGAGCTCGGTGAGACCATGGTGA
Protein sequence

>Msa0520260

MGASGKWVKALIGFKKPDKDEQHVKEGGKSKKWRLWRSSSGDNSSWKGFKTNHHKAASEGSESPTAAEAYTAAVATVVRAQPKDFRLVRQEWAAIRIQTTFRAFLARRALRALKAVVRIQALVRGRQVRKQAAVTLRCMQALVRVQARVRARRVRMSMEGQAVQNMLNERRSKLELLKEAEEGWCDSIGTLEDVKSKIQMRQEGAFKRERALAYSLAQKQCRPTSSTNSRTNTSFSTLRNHEMNKANGGWSWLERWMAAKPWETRLMEQSHAESLEKTPPPPPKKFAEPFVSSNLKPCSVKVKKNNVTTRISAKPPPHIGQATRSSSSPSSEFRYDESSASSSICTSTTPMSGNTCERTEDSCNSTRPNYMNLTQSTKAKLKSGSNQMYNRAQRQQSMDEFQFMKRAAVFSNGDSKSIAASDHSLNFSRPLHLPTQSDKSSVRPW*