AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0650200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650200 MtrunA17_Chr4g0042211 98.758 161 2 0 1 161 1 161 2.77e-113 318
Msa0650200 MtrunA17_Chr2g0280591 63.971 136 48 1 27 161 26 161 8.04e-58 177
Msa0650200 MtrunA17_Chr4g0048861 48.921 139 70 1 5 142 6 144 1.82e-44 143
Msa0650200 MtrunA17_Chr4g0048881 50.376 133 66 0 10 142 12 144 5.25e-44 143
Msa0650200 MtrunA17_Chr4g0048871 49.286 140 69 2 5 142 6 145 1.84e-43 141
Msa0650200 MtrunA17_Chr4g0031861 55.000 120 53 1 27 145 29 148 3.18e-42 138
Msa0650200 MtrunA17_Chr7g0249501 47.934 121 62 1 25 144 37 157 2.46e-37 126
Msa0650200 MtrunA17_Chr7g0234601 44.248 113 63 0 29 141 36 148 7.75e-34 117
Msa0650200 MtrunA17_Chr8g0378501 43.103 116 64 2 27 141 29 143 9.68e-29 103
Msa0650200 MtrunA17_Chr8g0378491 35.652 115 74 0 28 142 29 143 2.96e-24 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0650200 AT5G54530.1 62.121 132 50 0 28 159 28 159 1.70e-61 187
Msa0650200 AT1G61667.1 57.576 132 55 1 25 155 18 149 1.40e-52 164
Msa0650200 AT1G61667.2 57.576 132 55 1 25 155 54 185 4.35e-52 164
Msa0650200 AT3G07470.2 40.876 137 77 2 11 143 10 146 1.07e-32 114
Msa0650200 AT3G07470.3 40.876 137 77 2 11 143 10 146 1.31e-32 114
Msa0650200 AT3G07470.1 40.876 137 77 2 11 143 10 146 1.93e-32 114
Msa0650200 AT3G07460.1 40.876 137 79 2 6 141 8 143 4.81e-32 113
Msa0650200 AT3G07460.2 40.876 137 79 2 6 141 8 143 7.26e-31 112
Msa0650200 AT5G16380.1 43.796 137 72 3 10 141 8 144 5.21e-29 105
Msa0650200 AT1G55265.1 39.844 128 75 2 23 149 48 174 8.20e-28 102
Msa0650200 AT5G19860.1 34.711 121 79 0 25 145 32 152 1.18e-22 89.0
Msa0650200 AT1G02813.1 28.058 139 92 4 8 141 1 136 4.83e-11 58.2

Find 59 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATGTTCCTTTCTCTTTA+TGG 0.022892 4_3:-56988915 Msa0650200:exon
AAGGAACATGGAAGGGTTTA+AGG 0.156283 4_3:+56988926 None:intergenic
ATTGGTCTTTCTTGCATATC+TGG 0.216360 4_3:-56987375 Msa0650200:three_prime_UTR
ATGACTATGTTACCCTTCTT+TGG 0.279545 4_3:+56989054 None:intergenic
GTGTAGGACTTTATCTCTTC+TGG 0.280118 4_3:+56988767 None:intergenic
TTGTCATGTTTATATACTTT+TGG 0.301741 4_3:-56987568 Msa0650200:three_prime_UTR
AGAAGAGGAGAGAGAAAGTT+GGG 0.313243 4_3:+56988838 None:intergenic
TCCTACACTTTCTCAGAAAA+TGG 0.342412 4_3:-56988752 Msa0650200:CDS
TTGTGTTTGTGGGTCTTTGA+TGG 0.344987 4_3:+56989090 None:intergenic
ATTGTTGATGATCCATCTTC+TGG 0.353815 4_3:-56988569 Msa0650200:CDS
CATGAACTTCTTCGTTCAAA+AGG 0.359352 4_3:-56988809 Msa0650200:CDS
CCTTCTTTGGTTGGTGACTT+TGG 0.373102 4_3:+56989067 None:intergenic
GGTTTGATTCTCTTTGACAT+TGG 0.373655 4_3:-56988548 Msa0650200:CDS
GTGACTTTGGTTGTGTTTGT+GGG 0.384133 4_3:+56989080 None:intergenic
GGTGACTTTGGTTGTGTTTG+TGG 0.384303 4_3:+56989079 None:intergenic
GGAAGTCTCATTGGTGTTGA+AGG 0.392093 4_3:-56988638 Msa0650200:CDS
GAGGAAGGAGAGAGGATTTG+AGG 0.414030 4_3:-56987643 Msa0650200:CDS
AGAGGAGAAGGAGGAGAGAG+AGG 0.416690 4_3:+56988864 None:intergenic
TCAACAATGATATCTTTAAC+AGG 0.417805 4_3:+56988584 None:intergenic
AGAGAGAAAGTTGGGGAGAG+AGG 0.420739 4_3:+56988846 None:intergenic
TTTGTTCATTCACCAGCTAT+TGG 0.424105 4_3:-56987393 Msa0650200:three_prime_UTR
CTCACCTATGGAAGTCTCAT+TGG 0.430019 4_3:-56988647 Msa0650200:CDS
AAAGTTGGGGAGAGAGGAGA+AGG 0.432500 4_3:+56988852 None:intergenic
AAGAAGAGGAGAGAGAAAGT+TGG 0.438219 4_3:+56988837 None:intergenic
CCAAAGTCACCAACCAAAGA+AGG 0.456555 4_3:-56989067 Msa0650200:five_prime_UTR
GTTGGGGAGAGAGGAGAAGG+AGG 0.460848 4_3:+56988855 None:intergenic
AATCATGTGAGGAAGGAGAG+AGG 0.462940 4_3:-56987651 Msa0650200:CDS
CTATGTTACCCTTCTTTGGT+TGG 0.463103 4_3:+56989058 None:intergenic
AACTATAGTCAATATGTGTA+GGG 0.474579 4_3:-56987531 Msa0650200:three_prime_UTR
GTTGAAGAATCATGTGAGGA+AGG 0.477726 4_3:-56987658 Msa0650200:CDS
TTGTTAAACAAGGTGATTCA+AGG 0.482786 4_3:+56988714 None:intergenic
AAGAGAATCAAACCAGAAGA+TGG 0.489401 4_3:+56988557 None:intergenic
AAACTATAGTCAATATGTGT+AGG 0.490066 4_3:-56987532 Msa0650200:three_prime_UTR
TGTTTGAGAGAGAGATATGA+TGG 0.499117 4_3:+56988978 None:intergenic
ATAGAATGCCATAAAGAGAA+AGG 0.502220 4_3:+56988907 None:intergenic
AAGTTCATGGATGGAAGAAG+AGG 0.514641 4_3:+56988823 None:intergenic
AACATGTAAAGATGTGGGTG+GGG 0.517641 4_3:-56987470 Msa0650200:three_prime_UTR
TCTTCTGGTAAGAGACCAGC+TGG 0.524230 4_3:+56988782 None:intergenic
CGTTCAAAAGGCTTACCAGC+TGG 0.528273 4_3:-56988797 Msa0650200:CDS
GAACATGTAAAGATGTGGGT+GGG 0.529395 4_3:-56987471 Msa0650200:three_prime_UTR
ATCACTGCTAATCTCACCTA+TGG 0.534134 4_3:-56988659 Msa0650200:CDS
AGAGAGGATTTGAGGCAGTG+AGG 0.544712 4_3:-56987635 Msa0650200:CDS
TTGAAGCCTGAAAACTGAGT+TGG 0.544762 4_3:+56987600 None:intergenic
CCTTGAGGTTTACAATGAGG+TGG 0.551693 4_3:+56988485 None:intergenic
ATGCAAGAAAGACCAATAGC+TGG 0.569654 4_3:+56987381 None:intergenic
TGAACGAAGAAGTTCATGGA+TGG 0.572953 4_3:+56988814 None:intergenic
GAAGAGGAGAGAGAAAGTTG+GGG 0.580735 4_3:+56988839 None:intergenic
ATTAAGAACATGTAAAGATG+TGG 0.585266 4_3:-56987476 Msa0650200:three_prime_UTR
AAAGAGAAAGGAACATGGAA+GGG 0.586134 4_3:+56988919 None:intergenic
TAAAGAGAAAGGAACATGGA+AGG 0.596020 4_3:+56988918 None:intergenic
CCACCTCATTGTAAACCTCA+AGG 0.602779 4_3:-56988485 Msa0650200:intron
CAAAGTCACCAACCAAAGAA+GGG 0.606616 4_3:-56989066 Msa0650200:five_prime_UTR
TTACCTTGAGGTTTACAATG+AGG 0.607565 4_3:+56988482 None:intergenic
AGAACATGTAAAGATGTGGG+TGG 0.621050 4_3:-56987472 Msa0650200:three_prime_UTR
GCCATAAAGAGAAAGGAACA+TGG 0.627091 4_3:+56988914 None:intergenic
TTAAGAACATGTAAAGATGT+GGG 0.632160 4_3:-56987475 Msa0650200:three_prime_UTR
ACTATAGTCAATATGTGTAG+GGG 0.656085 4_3:-56987530 Msa0650200:three_prime_UTR
AACACCAATGAGACTTCCAT+AGG 0.677885 4_3:+56988643 None:intergenic
GTGAGTTGAAGAATCATGTG+AGG 0.690883 4_3:-56987662 Msa0650200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTCTTTCCAATTT+TGG - chr4_3:56988049-56988068 Msa0650200:intron 15.0%
!!! TTTTTTTTCTTTCCAATTTT+GGG - chr4_3:56988050-56988069 Msa0650200:intron 15.0%
!!! ATTTGCTTTTAATTATACAA+AGG + chr4_3:56988332-56988351 None:intergenic 15.0%
!! ATATATATAAAAGTCCAAAA+TGG - chr4_3:56988436-56988455 Msa0650200:intron 15.0%
!! TATATATAAAAGTCCAAAAT+GGG - chr4_3:56988437-56988456 Msa0650200:intron 15.0%
!!! TCTTTTTTACTTTTTGTTAA+AGG - chr4_3:56988966-56988985 Msa0650200:five_prime_UTR 15.0%
!!! TTTTTTTTTCTTTCCAATTT+TGG - chr4_3:56988049-56988068 Msa0650200:intron 15.0%
!!! TTTTTTTTCTTTCCAATTTT+GGG - chr4_3:56988050-56988069 Msa0650200:intron 15.0%
!!! ATTTGCTTTTAATTATACAA+AGG + chr4_3:56988332-56988351 None:intergenic 15.0%
!! ATATATATAAAAGTCCAAAA+TGG - chr4_3:56988436-56988455 Msa0650200:intron 15.0%
!! TATATATAAAAGTCCAAAAT+GGG - chr4_3:56988437-56988456 Msa0650200:intron 15.0%
!!! TCTTTTTTACTTTTTGTTAA+AGG - chr4_3:56988966-56988985 Msa0650200:five_prime_UTR 15.0%
!!! TTTTCATACTTTGTTAAACA+AGG + chr4_3:56987607-56987626 None:intergenic 20.0%
!!! TTGGAATTTTGTATTAATTG+AGG - chr4_3:56988101-56988120 Msa0650200:intron 20.0%
!!! TTCAATTTTTTTCATGTTTG+AGG - chr4_3:56988602-56988621 Msa0650200:CDS 20.0%
!!! TTGTCATGTTTATATACTTT+TGG - chr4_3:56988740-56988759 Msa0650200:CDS 20.0%
!!! TTTTCATACTTTGTTAAACA+AGG + chr4_3:56987607-56987626 None:intergenic 20.0%
!!! TTGGAATTTTGTATTAATTG+AGG - chr4_3:56988101-56988120 Msa0650200:intron 20.0%
!!! TTCAATTTTTTTCATGTTTG+AGG - chr4_3:56988602-56988621 Msa0650200:CDS 20.0%
!!! TTGTCATGTTTATATACTTT+TGG - chr4_3:56988740-56988759 Msa0650200:CDS 20.0%
! TCAACAATGATATCTTTAAC+AGG + chr4_3:56987727-56987746 None:intergenic 25.0%
!!! TCTAACTTTGTTATTGTCTT+TGG + chr4_3:56988278-56988297 None:intergenic 25.0%
! AATAACAAAGTTAGAAGAGA+AGG - chr4_3:56988283-56988302 Msa0650200:intron 25.0%
! AGAAGGAAAAAATAATTGAG+AGG - chr4_3:56988300-56988319 Msa0650200:intron 25.0%
!! TGTCATTATTTTCAACTTTG+CGG - chr4_3:56988529-56988548 Msa0650200:CDS 25.0%
! TATGTGTTGAAAGATGAAAA+TGG - chr4_3:56988559-56988578 Msa0650200:CDS 25.0%
! AAACTATAGTCAATATGTGT+AGG - chr4_3:56988776-56988795 Msa0650200:CDS 25.0%
! AACTATAGTCAATATGTGTA+GGG - chr4_3:56988777-56988796 Msa0650200:CDS 25.0%
! ATTAAGAACATGTAAAGATG+TGG - chr4_3:56988832-56988851 Msa0650200:CDS 25.0%
! TTAAGAACATGTAAAGATGT+GGG - chr4_3:56988833-56988852 Msa0650200:CDS 25.0%
! TCAACAATGATATCTTTAAC+AGG + chr4_3:56987727-56987746 None:intergenic 25.0%
!!! TCTAACTTTGTTATTGTCTT+TGG + chr4_3:56988278-56988297 None:intergenic 25.0%
! AATAACAAAGTTAGAAGAGA+AGG - chr4_3:56988283-56988302 Msa0650200:intron 25.0%
! AGAAGGAAAAAATAATTGAG+AGG - chr4_3:56988300-56988319 Msa0650200:intron 25.0%
!! TGTCATTATTTTCAACTTTG+CGG - chr4_3:56988529-56988548 Msa0650200:CDS 25.0%
! TATGTGTTGAAAGATGAAAA+TGG - chr4_3:56988559-56988578 Msa0650200:CDS 25.0%
! AAACTATAGTCAATATGTGT+AGG - chr4_3:56988776-56988795 Msa0650200:CDS 25.0%
! AACTATAGTCAATATGTGTA+GGG - chr4_3:56988777-56988796 Msa0650200:CDS 25.0%
! ATTAAGAACATGTAAAGATG+TGG - chr4_3:56988832-56988851 Msa0650200:CDS 25.0%
! TTAAGAACATGTAAAGATGT+GGG - chr4_3:56988833-56988852 Msa0650200:CDS 25.0%
ATAGAATGCCATAAAGAGAA+AGG + chr4_3:56987404-56987423 None:intergenic 30.0%
TTGTTAAACAAGGTGATTCA+AGG + chr4_3:56987597-56987616 None:intergenic 30.0%
! AACAAGAAGAGCTTTTTGTT+TGG - chr4_3:56987698-56987717 Msa0650200:intron 30.0%
GTTGAACAAAATTTACCTTG+AGG + chr4_3:56987841-56987860 None:intergenic 30.0%
! TATACTCCATGCACAATTTT+GGG - chr4_3:56987935-56987954 Msa0650200:intron 30.0%
AGCAAAAACAGTAGATTGAA+AGG + chr4_3:56987996-56988015 None:intergenic 30.0%
AACAAAACATCTCCCAAAAT+TGG + chr4_3:56988065-56988084 None:intergenic 30.0%
ATATAAAAGTCCAAAATGGG+TGG - chr4_3:56988440-56988459 Msa0650200:intron 30.0%
TATAAAAGTCCAAAATGGGT+GGG - chr4_3:56988441-56988460 Msa0650200:intron 30.0%
!! TTTTCATGTTTGAGGCTAAT+TGG - chr4_3:56988610-56988629 Msa0650200:CDS 30.0%
ACTATAGTCAATATGTGTAG+GGG - chr4_3:56988778-56988797 Msa0650200:CDS 30.0%
!!! GTTTTTTAGTGTAAAGGTTG+AGG - chr4_3:56988800-56988819 Msa0650200:CDS 30.0%
ATAGAATGCCATAAAGAGAA+AGG + chr4_3:56987404-56987423 None:intergenic 30.0%
TTGTTAAACAAGGTGATTCA+AGG + chr4_3:56987597-56987616 None:intergenic 30.0%
! AACAAGAAGAGCTTTTTGTT+TGG - chr4_3:56987698-56987717 Msa0650200:intron 30.0%
GTTGAACAAAATTTACCTTG+AGG + chr4_3:56987841-56987860 None:intergenic 30.0%
! TATACTCCATGCACAATTTT+GGG - chr4_3:56987935-56987954 Msa0650200:intron 30.0%
AGCAAAAACAGTAGATTGAA+AGG + chr4_3:56987996-56988015 None:intergenic 30.0%
AACAAAACATCTCCCAAAAT+TGG + chr4_3:56988065-56988084 None:intergenic 30.0%
ATATAAAAGTCCAAAATGGG+TGG - chr4_3:56988440-56988459 Msa0650200:intron 30.0%
TATAAAAGTCCAAAATGGGT+GGG - chr4_3:56988441-56988460 Msa0650200:intron 30.0%
!! TTTTCATGTTTGAGGCTAAT+TGG - chr4_3:56988610-56988629 Msa0650200:CDS 30.0%
ACTATAGTCAATATGTGTAG+GGG - chr4_3:56988778-56988797 Msa0650200:CDS 30.0%
!!! GTTTTTTAGTGTAAAGGTTG+AGG - chr4_3:56988800-56988819 Msa0650200:CDS 30.0%
ATGACTATGTTACCCTTCTT+TGG + chr4_3:56987257-56987276 None:intergenic 35.0%
TGTTTGAGAGAGAGATATGA+TGG + chr4_3:56987333-56987352 None:intergenic 35.0%
AAAGAGAAAGGAACATGGAA+GGG + chr4_3:56987392-56987411 None:intergenic 35.0%
TAAAGAGAAAGGAACATGGA+AGG + chr4_3:56987393-56987412 None:intergenic 35.0%
TCCATGTTCCTTTCTCTTTA+TGG - chr4_3:56987393-56987412 Msa0650200:three_prime_UTR 35.0%
! CTTTTGAACGAAGAAGTTCA+TGG + chr4_3:56987501-56987520 None:intergenic 35.0%
CATGAACTTCTTCGTTCAAA+AGG - chr4_3:56987499-56987518 Msa0650200:three_prime_UTR 35.0%
TCCTACACTTTCTCAGAAAA+TGG - chr4_3:56987556-56987575 Msa0650200:three_prime_UTR 35.0%
!! ACCATTTTCTGAGAAAGTGT+AGG + chr4_3:56987560-56987579 None:intergenic 35.0%
!! ATTGTTGATGATCCATCTTC+TGG - chr4_3:56987739-56987758 Msa0650200:intron 35.0%
AAGAGAATCAAACCAGAAGA+TGG + chr4_3:56987754-56987773 None:intergenic 35.0%
GGTTTGATTCTCTTTGACAT+TGG - chr4_3:56987760-56987779 Msa0650200:intron 35.0%
TTACCTTGAGGTTTACAATG+AGG + chr4_3:56987829-56987848 None:intergenic 35.0%
ATGGAGTATAGTTCCAAAAG+GGG + chr4_3:56987925-56987944 None:intergenic 35.0%
CATGGAGTATAGTTCCAAAA+GGG + chr4_3:56987926-56987945 None:intergenic 35.0%
! CTATACTCCATGCACAATTT+TGG - chr4_3:56987934-56987953 Msa0650200:intron 35.0%
TTATGACCCAAAATTGTGCA+TGG + chr4_3:56987944-56987963 None:intergenic 35.0%
GTTGAGTGTGAAATGTTCTT+TGG - chr4_3:56988082-56988101 Msa0650200:intron 35.0%
ATAAAAGTCCAAAATGGGTG+GGG - chr4_3:56988442-56988461 Msa0650200:intron 35.0%
!!! GTAGGGGTTTTTTAGTGTAA+AGG - chr4_3:56988794-56988813 Msa0650200:CDS 35.0%
! TTTTAGTGTAAAGGTTGAGG+AGG - chr4_3:56988803-56988822 Msa0650200:CDS 35.0%
TTTGTTCATTCACCAGCTAT+TGG - chr4_3:56988915-56988934 Msa0650200:exon 35.0%
!! ATTGGTCTTTCTTGCATATC+TGG - chr4_3:56988933-56988952 Msa0650200:five_prime_UTR 35.0%
ATGACTATGTTACCCTTCTT+TGG + chr4_3:56987257-56987276 None:intergenic 35.0%
TGTTTGAGAGAGAGATATGA+TGG + chr4_3:56987333-56987352 None:intergenic 35.0%
AAAGAGAAAGGAACATGGAA+GGG + chr4_3:56987392-56987411 None:intergenic 35.0%
TAAAGAGAAAGGAACATGGA+AGG + chr4_3:56987393-56987412 None:intergenic 35.0%
TCCATGTTCCTTTCTCTTTA+TGG - chr4_3:56987393-56987412 Msa0650200:three_prime_UTR 35.0%
! CTTTTGAACGAAGAAGTTCA+TGG + chr4_3:56987501-56987520 None:intergenic 35.0%
CATGAACTTCTTCGTTCAAA+AGG - chr4_3:56987499-56987518 Msa0650200:three_prime_UTR 35.0%
TCCTACACTTTCTCAGAAAA+TGG - chr4_3:56987556-56987575 Msa0650200:three_prime_UTR 35.0%
!! ACCATTTTCTGAGAAAGTGT+AGG + chr4_3:56987560-56987579 None:intergenic 35.0%
!! ATTGTTGATGATCCATCTTC+TGG - chr4_3:56987739-56987758 Msa0650200:intron 35.0%
AAGAGAATCAAACCAGAAGA+TGG + chr4_3:56987754-56987773 None:intergenic 35.0%
GGTTTGATTCTCTTTGACAT+TGG - chr4_3:56987760-56987779 Msa0650200:intron 35.0%
TTACCTTGAGGTTTACAATG+AGG + chr4_3:56987829-56987848 None:intergenic 35.0%
ATGGAGTATAGTTCCAAAAG+GGG + chr4_3:56987925-56987944 None:intergenic 35.0%
CATGGAGTATAGTTCCAAAA+GGG + chr4_3:56987926-56987945 None:intergenic 35.0%
! CTATACTCCATGCACAATTT+TGG - chr4_3:56987934-56987953 Msa0650200:intron 35.0%
TTATGACCCAAAATTGTGCA+TGG + chr4_3:56987944-56987963 None:intergenic 35.0%
GTTGAGTGTGAAATGTTCTT+TGG - chr4_3:56988082-56988101 Msa0650200:intron 35.0%
ATAAAAGTCCAAAATGGGTG+GGG - chr4_3:56988442-56988461 Msa0650200:intron 35.0%
!!! GTAGGGGTTTTTTAGTGTAA+AGG - chr4_3:56988794-56988813 Msa0650200:CDS 35.0%
! TTTTAGTGTAAAGGTTGAGG+AGG - chr4_3:56988803-56988822 Msa0650200:CDS 35.0%
TTTGTTCATTCACCAGCTAT+TGG - chr4_3:56988915-56988934 Msa0650200:exon 35.0%
!! ATTGGTCTTTCTTGCATATC+TGG - chr4_3:56988933-56988952 Msa0650200:five_prime_UTR 35.0%
GTGACTTTGGTTGTGTTTGT+GGG + chr4_3:56987231-56987250 None:intergenic 40.0%
CAAAGTCACCAACCAAAGAA+GGG - chr4_3:56987242-56987261 Msa0650200:three_prime_UTR 40.0%
CTATGTTACCCTTCTTTGGT+TGG + chr4_3:56987253-56987272 None:intergenic 40.0%
AAGGAACATGGAAGGGTTTA+AGG + chr4_3:56987385-56987404 None:intergenic 40.0%
GCCATAAAGAGAAAGGAACA+TGG + chr4_3:56987397-56987416 None:intergenic 40.0%
AGAAGAGGAGAGAGAAAGTT+GGG + chr4_3:56987473-56987492 None:intergenic 40.0%
AAGAAGAGGAGAGAGAAAGT+TGG + chr4_3:56987474-56987493 None:intergenic 40.0%
AAGTTCATGGATGGAAGAAG+AGG + chr4_3:56987488-56987507 None:intergenic 40.0%
TGAACGAAGAAGTTCATGGA+TGG + chr4_3:56987497-56987516 None:intergenic 40.0%
GTGTAGGACTTTATCTCTTC+TGG + chr4_3:56987544-56987563 None:intergenic 40.0%
ATCACTGCTAATCTCACCTA+TGG - chr4_3:56987649-56987668 Msa0650200:CDS 40.0%
AACACCAATGAGACTTCCAT+AGG + chr4_3:56987668-56987687 None:intergenic 40.0%
! AGATCTGCTATTACCCCTTT+TGG - chr4_3:56987909-56987928 Msa0650200:intron 40.0%
GCATGGAGTATAGTTCCAAA+AGG + chr4_3:56987927-56987946 None:intergenic 40.0%
! TAAATTAGCCCCACCCATTT+TGG + chr4_3:56988453-56988472 None:intergenic 40.0%
! GTGAGTTGAAGAATCATGTG+AGG - chr4_3:56988646-56988665 Msa0650200:CDS 40.0%
GTTGAAGAATCATGTGAGGA+AGG - chr4_3:56988650-56988669 Msa0650200:CDS 40.0%
! AAGATGCCAACTCAGTTTTC+AGG - chr4_3:56988702-56988721 Msa0650200:CDS 40.0%
TTGAAGCCTGAAAACTGAGT+TGG + chr4_3:56988711-56988730 None:intergenic 40.0%
AGAACATGTAAAGATGTGGG+TGG - chr4_3:56988836-56988855 Msa0650200:CDS 40.0%
GAACATGTAAAGATGTGGGT+GGG - chr4_3:56988837-56988856 Msa0650200:CDS 40.0%
AACATGTAAAGATGTGGGTG+GGG - chr4_3:56988838-56988857 Msa0650200:CDS 40.0%
ATGCAAGAAAGACCAATAGC+TGG + chr4_3:56988930-56988949 None:intergenic 40.0%
GTGACTTTGGTTGTGTTTGT+GGG + chr4_3:56987231-56987250 None:intergenic 40.0%
CAAAGTCACCAACCAAAGAA+GGG - chr4_3:56987242-56987261 Msa0650200:three_prime_UTR 40.0%
CTATGTTACCCTTCTTTGGT+TGG + chr4_3:56987253-56987272 None:intergenic 40.0%
AAGGAACATGGAAGGGTTTA+AGG + chr4_3:56987385-56987404 None:intergenic 40.0%
GCCATAAAGAGAAAGGAACA+TGG + chr4_3:56987397-56987416 None:intergenic 40.0%
AGAAGAGGAGAGAGAAAGTT+GGG + chr4_3:56987473-56987492 None:intergenic 40.0%
AAGAAGAGGAGAGAGAAAGT+TGG + chr4_3:56987474-56987493 None:intergenic 40.0%
AAGTTCATGGATGGAAGAAG+AGG + chr4_3:56987488-56987507 None:intergenic 40.0%
TGAACGAAGAAGTTCATGGA+TGG + chr4_3:56987497-56987516 None:intergenic 40.0%
GTGTAGGACTTTATCTCTTC+TGG + chr4_3:56987544-56987563 None:intergenic 40.0%
ATCACTGCTAATCTCACCTA+TGG - chr4_3:56987649-56987668 Msa0650200:CDS 40.0%
AACACCAATGAGACTTCCAT+AGG + chr4_3:56987668-56987687 None:intergenic 40.0%
! AGATCTGCTATTACCCCTTT+TGG - chr4_3:56987909-56987928 Msa0650200:intron 40.0%
GCATGGAGTATAGTTCCAAA+AGG + chr4_3:56987927-56987946 None:intergenic 40.0%
! TAAATTAGCCCCACCCATTT+TGG + chr4_3:56988453-56988472 None:intergenic 40.0%
! GTGAGTTGAAGAATCATGTG+AGG - chr4_3:56988646-56988665 Msa0650200:CDS 40.0%
GTTGAAGAATCATGTGAGGA+AGG - chr4_3:56988650-56988669 Msa0650200:CDS 40.0%
! AAGATGCCAACTCAGTTTTC+AGG - chr4_3:56988702-56988721 Msa0650200:CDS 40.0%
TTGAAGCCTGAAAACTGAGT+TGG + chr4_3:56988711-56988730 None:intergenic 40.0%
AGAACATGTAAAGATGTGGG+TGG - chr4_3:56988836-56988855 Msa0650200:CDS 40.0%
GAACATGTAAAGATGTGGGT+GGG - chr4_3:56988837-56988856 Msa0650200:CDS 40.0%
AACATGTAAAGATGTGGGTG+GGG - chr4_3:56988838-56988857 Msa0650200:CDS 40.0%
ATGCAAGAAAGACCAATAGC+TGG + chr4_3:56988930-56988949 None:intergenic 40.0%
GGTGACTTTGGTTGTGTTTG+TGG + chr4_3:56987232-56987251 None:intergenic 45.0%
!! CCTTCTTTGGTTGGTGACTT+TGG + chr4_3:56987244-56987263 None:intergenic 45.0%
CCAAAGTCACCAACCAAAGA+AGG - chr4_3:56987241-56987260 Msa0650200:three_prime_UTR 45.0%
GAAGAGGAGAGAGAAAGTTG+GGG + chr4_3:56987472-56987491 None:intergenic 45.0%
CTCACCTATGGAAGTCTCAT+TGG - chr4_3:56987661-56987680 Msa0650200:CDS 45.0%
!! GGAAGTCTCATTGGTGTTGA+AGG - chr4_3:56987670-56987689 Msa0650200:intron 45.0%
CCTTGAGGTTTACAATGAGG+TGG + chr4_3:56987826-56987845 None:intergenic 45.0%
CCACCTCATTGTAAACCTCA+AGG - chr4_3:56987823-56987842 Msa0650200:intron 45.0%
! GCTAATTGGTTGAGTTGTGC+AGG - chr4_3:56988624-56988643 Msa0650200:CDS 45.0%
AATCATGTGAGGAAGGAGAG+AGG - chr4_3:56988657-56988676 Msa0650200:CDS 45.0%
GGTGACTTTGGTTGTGTTTG+TGG + chr4_3:56987232-56987251 None:intergenic 45.0%
!! CCTTCTTTGGTTGGTGACTT+TGG + chr4_3:56987244-56987263 None:intergenic 45.0%
CCAAAGTCACCAACCAAAGA+AGG - chr4_3:56987241-56987260 Msa0650200:three_prime_UTR 45.0%
GAAGAGGAGAGAGAAAGTTG+GGG + chr4_3:56987472-56987491 None:intergenic 45.0%
CTCACCTATGGAAGTCTCAT+TGG - chr4_3:56987661-56987680 Msa0650200:CDS 45.0%
!! GGAAGTCTCATTGGTGTTGA+AGG - chr4_3:56987670-56987689 Msa0650200:intron 45.0%
CCTTGAGGTTTACAATGAGG+TGG + chr4_3:56987826-56987845 None:intergenic 45.0%
CCACCTCATTGTAAACCTCA+AGG - chr4_3:56987823-56987842 Msa0650200:intron 45.0%
! GCTAATTGGTTGAGTTGTGC+AGG - chr4_3:56988624-56988643 Msa0650200:CDS 45.0%
AATCATGTGAGGAAGGAGAG+AGG - chr4_3:56988657-56988676 Msa0650200:CDS 45.0%
AAAGTTGGGGAGAGAGGAGA+AGG + chr4_3:56987459-56987478 None:intergenic 50.0%
AGAGAGAAAGTTGGGGAGAG+AGG + chr4_3:56987465-56987484 None:intergenic 50.0%
CGTTCAAAAGGCTTACCAGC+TGG - chr4_3:56987511-56987530 Msa0650200:three_prime_UTR 50.0%
TCTTCTGGTAAGAGACCAGC+TGG + chr4_3:56987529-56987548 None:intergenic 50.0%
GAGGAAGGAGAGAGGATTTG+AGG - chr4_3:56988665-56988684 Msa0650200:CDS 50.0%
! AGAGAGGATTTGAGGCAGTG+AGG - chr4_3:56988673-56988692 Msa0650200:CDS 50.0%
AAAGTTGGGGAGAGAGGAGA+AGG + chr4_3:56987459-56987478 None:intergenic 50.0%
AGAGAGAAAGTTGGGGAGAG+AGG + chr4_3:56987465-56987484 None:intergenic 50.0%
CGTTCAAAAGGCTTACCAGC+TGG - chr4_3:56987511-56987530 Msa0650200:three_prime_UTR 50.0%
TCTTCTGGTAAGAGACCAGC+TGG + chr4_3:56987529-56987548 None:intergenic 50.0%
GAGGAAGGAGAGAGGATTTG+AGG - chr4_3:56988665-56988684 Msa0650200:CDS 50.0%
! AGAGAGGATTTGAGGCAGTG+AGG - chr4_3:56988673-56988692 Msa0650200:CDS 50.0%
AGAGGAGAAGGAGGAGAGAG+AGG + chr4_3:56987447-56987466 None:intergenic 55.0%
AGAGGAGAAGGAGGAGAGAG+AGG + chr4_3:56987447-56987466 None:intergenic 55.0%
GTTGGGGAGAGAGGAGAAGG+AGG + chr4_3:56987456-56987475 None:intergenic 60.0%
GTTGGGGAGAGAGGAGAAGG+AGG + chr4_3:56987456-56987475 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 56987220 56989110 56987220 ID=Msa0650200;Name=Msa0650200
chr4_3 mRNA 56987220 56989110 56987220 ID=Msa0650200-mRNA-1;Parent=Msa0650200;Name=Msa0650200-mRNA-1;_AED=0.02;_eAED=0.02;_QI=192|1|1|1|1|1|2|412|161
chr4_3 exon 56987220 56987684 56987220 ID=Msa0650200-mRNA-1:exon:14936;Parent=Msa0650200-mRNA-1
chr4_3 exon 56988486 56989110 56988486 ID=Msa0650200-mRNA-1:exon:14935;Parent=Msa0650200-mRNA-1
chr4_3 five_prime_UTR 56988919 56989110 56988919 ID=Msa0650200-mRNA-1:five_prime_utr;Parent=Msa0650200-mRNA-1
chr4_3 CDS 56988486 56988918 56988486 ID=Msa0650200-mRNA-1:cds;Parent=Msa0650200-mRNA-1
chr4_3 CDS 56987632 56987684 56987632 ID=Msa0650200-mRNA-1:cds;Parent=Msa0650200-mRNA-1
chr4_3 three_prime_UTR 56987220 56987631 56987220 ID=Msa0650200-mRNA-1:three_prime_utr;Parent=Msa0650200-mRNA-1
Gene Sequence

>Msa0650200

ATGGCATTCTATAACACTCTTCTCTCTTTCTTCCTCTCTCTCCTCCTTCTCCTCTCTCCCCAACTTTCTCTCTCCTCTTCTTCCATCCATGAACTTCTTCGTTCAAAAGGCTTACCAGCTGGTCTCTTACCAGAAGAGATAAAGTCCTACACTTTCTCAGAAAATGGTCACTTAGAAGTGTTCCTTGAATCACCTTGTTTAACAAAGTATGAAAACAGAGTCTACTTTGAACAAGTTATCACTGCTAATCTCACCTATGGAAGTCTCATTGGTGTTGAAGGTTTGCAACAAGAAGAGCTTTTTGTTTGGTTACCTGTTAAAGATATCATTGTTGATGATCCATCTTCTGGTTTGATTCTCTTTGACATTGGTCTTGCTTATAAACAACTCTCTTTCTCTCTCTTTGAAGTTCCACCTCATTGTAAACCTCAAGGTGAGTTGAAGAATCATGTGAGGAAGGAGAGAGGATTTGAGGCAGTGAGGTAG
Protein sequence

>Msa0650200

MAFYNTLLSFFLSLLLLLSPQLSLSSSSIHELLRSKGLPAGLLPEEIKSYTFSENGHLEVFLESPCLTKYENRVYFEQVITANLTYGSLIGVEGLQQEELFVWLPVKDIIVDDPSSGLILFDIGLAYKQLSFSLFEVPPHCKPQGELKNHVRKERGFEAVR*