AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0609650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609650 MtrunA17_Chr4g0042211 98.137 161 3 0 1 161 1 161 2.80e-112 315
Msa0609650 MtrunA17_Chr2g0280591 63.971 136 48 1 27 161 26 161 1.27e-57 177
Msa0609650 MtrunA17_Chr4g0048861 48.921 139 70 1 5 142 6 144 2.10e-44 143
Msa0609650 MtrunA17_Chr4g0048881 50.376 133 66 0 10 142 12 144 5.20e-44 143
Msa0609650 MtrunA17_Chr4g0048871 49.286 140 69 2 5 142 6 145 2.05e-43 141
Msa0609650 MtrunA17_Chr4g0031861 52.632 133 61 1 27 157 29 161 8.50e-43 139
Msa0609650 MtrunA17_Chr7g0249501 47.934 121 62 1 25 144 37 157 2.54e-37 125
Msa0609650 MtrunA17_Chr7g0234601 44.248 113 63 0 29 141 36 148 8.37e-34 117
Msa0609650 MtrunA17_Chr8g0378501 41.667 120 68 2 27 145 29 147 1.21e-28 103
Msa0609650 MtrunA17_Chr8g0378491 35.652 115 74 0 28 142 29 143 3.33e-24 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609650 AT5G54530.1 62.121 132 50 0 28 159 28 159 6.06e-61 186
Msa0609650 AT1G61667.1 57.576 132 55 1 25 155 18 149 2.41e-52 164
Msa0609650 AT1G61667.2 57.576 132 55 1 25 155 54 185 4.80e-52 164
Msa0609650 AT3G07470.2 40.876 137 77 2 11 143 10 146 1.40e-32 114
Msa0609650 AT3G07470.3 40.876 137 77 2 11 143 10 146 1.58e-32 114
Msa0609650 AT3G07470.1 40.876 137 77 2 11 143 10 146 2.38e-32 113
Msa0609650 AT3G07460.1 40.876 137 79 2 6 141 8 143 5.78e-32 112
Msa0609650 AT3G07460.2 40.876 137 79 2 6 141 8 143 9.27e-31 112
Msa0609650 AT5G16380.1 43.796 137 72 3 10 141 8 144 6.33e-29 105
Msa0609650 AT1G55265.1 39.844 128 75 2 23 149 48 174 9.44e-28 102
Msa0609650 AT5G19860.1 33.094 139 86 1 25 156 32 170 2.91e-23 90.9
Msa0609650 AT1G02813.1 28.058 139 92 4 8 141 1 136 5.53e-11 57.8

Find 63 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATGTTCCTTTCTCTTTA+TGG 0.022892 4_2:+56977329 Msa0609650:exon
AAGGAACATGGAAGGGTTTA+AGG 0.156283 4_2:-56977318 None:intergenic
ATTGGTCTTTCTTGCATATC+TGG 0.216360 4_2:+56978878 Msa0609650:three_prime_UTR
ATGACTATGTTACCCTTCTT+TGG 0.279545 4_2:-56977185 None:intergenic
GTGTAGGACTTTATCTCTTC+TGG 0.280118 4_2:-56977477 None:intergenic
TTGTCATGTTTATATACTTT+TGG 0.301741 4_2:+56978685 Msa0609650:three_prime_UTR
AGAAGAGGAGAGAGAAAGTT+GGG 0.313243 4_2:-56977406 None:intergenic
TCCTACACTTTCTCAGAAAA+TGG 0.342412 4_2:+56977492 Msa0609650:CDS
TTGTGTTTGTGGGTCTTTGA+TGG 0.344987 4_2:-56977149 None:intergenic
ATTGTTGATGATCCATCTTC+TGG 0.353815 4_2:+56977675 Msa0609650:CDS
GGAAGGGTTTAAGGTTAAGT+TGG 0.355475 4_2:-56977309 None:intergenic
CATGAACTTCTTCGTTCAAA+AGG 0.359352 4_2:+56977435 Msa0609650:CDS
CCTTCTTTGGTTGGTGACTT+TGG 0.373102 4_2:-56977172 None:intergenic
GTGACTTTGGTTGTGTTTGT+GGG 0.384133 4_2:-56977159 None:intergenic
GGTGACTTTGGTTGTGTTTG+TGG 0.384303 4_2:-56977160 None:intergenic
GGAAGTCTCATTGGTGTTGA+AGG 0.392093 4_2:+56977606 Msa0609650:CDS
GAGGAAGGGGAGAGGATTTG+AGG 0.396002 4_2:+56978604 Msa0609650:CDS
AGAGGAGAAGGAGGAGAGAG+AGG 0.416690 4_2:-56977380 None:intergenic
TCAACAATGATATCTTTAAC+AGG 0.417805 4_2:-56977660 None:intergenic
AGAGAGAAAGTTGGGGAGAG+AGG 0.420739 4_2:-56977398 None:intergenic
TTTGTTCATTCACCAGCTAT+TGG 0.424105 4_2:+56978860 Msa0609650:three_prime_UTR
CTCACCTATGGAAGTCTCAT+TGG 0.430019 4_2:+56977597 Msa0609650:CDS
GGTTTGATTCTCTTCGACAT+TGG 0.430179 4_2:+56977696 Msa0609650:CDS
AAAGTTGGGGAGAGAGGAGA+AGG 0.432500 4_2:-56977392 None:intergenic
GAAGGGTTTAAGGTTAAGTT+GGG 0.433298 4_2:-56977308 None:intergenic
AAGAAGAGGAGAGAGAAAGT+TGG 0.438219 4_2:-56977407 None:intergenic
CCAAAGTCACCAACCAAAGA+AGG 0.456555 4_2:+56977172 Msa0609650:five_prime_UTR
GTTGGGGAGAGAGGAGAAGG+AGG 0.460848 4_2:-56977389 None:intergenic
CTATGTTACCCTTCTTTGGT+TGG 0.463103 4_2:-56977181 None:intergenic
TGAAGAATCATGTGAGGAAG+GGG 0.465571 4_2:+56978591 Msa0609650:CDS
AACTATAGTCAATATGTGTA+GGG 0.474579 4_2:+56978722 Msa0609650:three_prime_UTR
TTGTTAAACAAGGTGATTCA+AGG 0.482786 4_2:-56977530 None:intergenic
AATCATGTGAGGAAGGGGAG+AGG 0.487370 4_2:+56978596 Msa0609650:CDS
AAACTATAGTCAATATGTGT+AGG 0.490066 4_2:+56978721 Msa0609650:three_prime_UTR
AAGAGAATCAAACCAGAAGA+TGG 0.492142 4_2:-56977687 None:intergenic
TGTTTGAGAGAGAGATATGA+TGG 0.499117 4_2:-56977261 None:intergenic
ATAGAATGCCATAAAGAGAA+AGG 0.502220 4_2:-56977337 None:intergenic
AAGTTCATGGATGGAAGAAG+AGG 0.514641 4_2:-56977421 None:intergenic
AACATGTAAAGATGTGGGTG+GGG 0.517641 4_2:+56978783 Msa0609650:three_prime_UTR
GTTGAAGAATCATGTGAGGA+AGG 0.518935 4_2:+56978589 Msa0609650:CDS
TTGAAGAATCATGTGAGGAA+GGG 0.521918 4_2:+56978590 Msa0609650:CDS
TCTTCTGGTAAGAGACCAGC+TGG 0.524230 4_2:-56977462 None:intergenic
CGTTCAAAAGGCTTACCAGC+TGG 0.528273 4_2:+56977447 Msa0609650:CDS
GAACATGTAAAGATGTGGGT+GGG 0.529395 4_2:+56978782 Msa0609650:three_prime_UTR
ATCACTGCTAATCTCACCTA+TGG 0.534134 4_2:+56977585 Msa0609650:CDS
TTGAAGCCTGAAAACTGAGT+TGG 0.544762 4_2:-56978647 None:intergenic
CCTTGAGGTTTACAATGAGG+TGG 0.551693 4_2:-56977759 None:intergenic
GGAGAGGATTTGAGGCAGTG+AGG 0.554342 4_2:+56978612 Msa0609650:CDS
ATGCAAGAAAGACCAATAGC+TGG 0.569654 4_2:-56978872 None:intergenic
TGAACGAAGAAGTTCATGGA+TGG 0.572953 4_2:-56977430 None:intergenic
GAAGAGGAGAGAGAAAGTTG+GGG 0.580735 4_2:-56977405 None:intergenic
ATTAAGAACATGTAAAGATG+TGG 0.585266 4_2:+56978777 Msa0609650:three_prime_UTR
AAAGAGAAAGGAACATGGAA+GGG 0.586134 4_2:-56977325 None:intergenic
TAAAGAGAAAGGAACATGGA+AGG 0.596020 4_2:-56977326 None:intergenic
CCACCTCATTGTAAACCTCA+AGG 0.602779 4_2:+56977759 Msa0609650:CDS
CAAAGTCACCAACCAAAGAA+GGG 0.606616 4_2:+56977173 Msa0609650:five_prime_UTR
TTACCTTGAGGTTTACAATG+AGG 0.607565 4_2:-56977762 None:intergenic
AGAACATGTAAAGATGTGGG+TGG 0.621050 4_2:+56978781 Msa0609650:three_prime_UTR
GCCATAAAGAGAAAGGAACA+TGG 0.627091 4_2:-56977330 None:intergenic
TTAAGAACATGTAAAGATGT+GGG 0.632160 4_2:+56978778 Msa0609650:three_prime_UTR
ACTATAGTCAATATGTGTAG+GGG 0.656085 4_2:+56978723 Msa0609650:three_prime_UTR
AACACCAATGAGACTTCCAT+AGG 0.677885 4_2:-56977601 None:intergenic
GTGAGTTGAAGAATCATGTG+AGG 0.690883 4_2:+56978585 Msa0609650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTCTTTCCAATTT+TGG + chr4_2:56977988-56978007 Msa0609650:intron 15.0%
!!! TTTTTTTTCTTTCCAATTTT+GGG + chr4_2:56977989-56978008 Msa0609650:intron 15.0%
!!! ATTTGCTTTTAATTATACAA+AGG - chr4_2:56978271-56978290 None:intergenic 15.0%
!! ATATATATAAAAGTCCAAAA+TGG + chr4_2:56978375-56978394 Msa0609650:intron 15.0%
!! TATATATAAAAGTCCAAAAT+GGG + chr4_2:56978376-56978395 Msa0609650:intron 15.0%
!!! TCTTTTTTACTTTTTGTTAA+AGG + chr4_2:56978911-56978930 Msa0609650:three_prime_UTR 15.0%
!!! TTTTTTTTTCTTTCCAATTT+TGG + chr4_2:56977988-56978007 Msa0609650:intron 15.0%
!!! TTTTTTTTCTTTCCAATTTT+GGG + chr4_2:56977989-56978008 Msa0609650:intron 15.0%
!!! ATTTGCTTTTAATTATACAA+AGG - chr4_2:56978271-56978290 None:intergenic 15.0%
!! ATATATATAAAAGTCCAAAA+TGG + chr4_2:56978375-56978394 Msa0609650:intron 15.0%
!! TATATATAAAAGTCCAAAAT+GGG + chr4_2:56978376-56978395 Msa0609650:intron 15.0%
!!! TCTTTTTTACTTTTTGTTAA+AGG + chr4_2:56978911-56978930 Msa0609650:three_prime_UTR 15.0%
!!! TTTTCATACTTTGTTAAACA+AGG - chr4_2:56977543-56977562 None:intergenic 20.0%
!!! TTGGAATTTTGTATTAATTG+AGG + chr4_2:56978040-56978059 Msa0609650:intron 20.0%
!!! TTCAATTTTTTTCATGTTTG+AGG + chr4_2:56978541-56978560 Msa0609650:intron 20.0%
!!! TTGTCATGTTTATATACTTT+TGG + chr4_2:56978685-56978704 Msa0609650:three_prime_UTR 20.0%
!!! TTTTCATACTTTGTTAAACA+AGG - chr4_2:56977543-56977562 None:intergenic 20.0%
!!! TTGGAATTTTGTATTAATTG+AGG + chr4_2:56978040-56978059 Msa0609650:intron 20.0%
!!! TTCAATTTTTTTCATGTTTG+AGG + chr4_2:56978541-56978560 Msa0609650:intron 20.0%
!!! TTGTCATGTTTATATACTTT+TGG + chr4_2:56978685-56978704 Msa0609650:three_prime_UTR 20.0%
! TCAACAATGATATCTTTAAC+AGG - chr4_2:56977663-56977682 None:intergenic 25.0%
!!! TCTAACTTTGTTATTGTCTT+TGG - chr4_2:56978217-56978236 None:intergenic 25.0%
! AATAACAAAGTTAGAAGAGA+AGG + chr4_2:56978222-56978241 Msa0609650:intron 25.0%
! AGAAGGAAAAAATAATTGAG+AGG + chr4_2:56978239-56978258 Msa0609650:intron 25.0%
!! TGTCATTATTTTCAACTTTG+CGG + chr4_2:56978468-56978487 Msa0609650:intron 25.0%
! TATGTGTTGAAAGATGAAAA+TGG + chr4_2:56978498-56978517 Msa0609650:intron 25.0%
! AAACTATAGTCAATATGTGT+AGG + chr4_2:56978721-56978740 Msa0609650:three_prime_UTR 25.0%
! AACTATAGTCAATATGTGTA+GGG + chr4_2:56978722-56978741 Msa0609650:three_prime_UTR 25.0%
! ATTAAGAACATGTAAAGATG+TGG + chr4_2:56978777-56978796 Msa0609650:three_prime_UTR 25.0%
! TTAAGAACATGTAAAGATGT+GGG + chr4_2:56978778-56978797 Msa0609650:three_prime_UTR 25.0%
! TCAACAATGATATCTTTAAC+AGG - chr4_2:56977663-56977682 None:intergenic 25.0%
!!! TCTAACTTTGTTATTGTCTT+TGG - chr4_2:56978217-56978236 None:intergenic 25.0%
! AATAACAAAGTTAGAAGAGA+AGG + chr4_2:56978222-56978241 Msa0609650:intron 25.0%
! AGAAGGAAAAAATAATTGAG+AGG + chr4_2:56978239-56978258 Msa0609650:intron 25.0%
!! TGTCATTATTTTCAACTTTG+CGG + chr4_2:56978468-56978487 Msa0609650:intron 25.0%
! TATGTGTTGAAAGATGAAAA+TGG + chr4_2:56978498-56978517 Msa0609650:intron 25.0%
! AAACTATAGTCAATATGTGT+AGG + chr4_2:56978721-56978740 Msa0609650:three_prime_UTR 25.0%
! AACTATAGTCAATATGTGTA+GGG + chr4_2:56978722-56978741 Msa0609650:three_prime_UTR 25.0%
! ATTAAGAACATGTAAAGATG+TGG + chr4_2:56978777-56978796 Msa0609650:three_prime_UTR 25.0%
! TTAAGAACATGTAAAGATGT+GGG + chr4_2:56978778-56978797 Msa0609650:three_prime_UTR 25.0%
ATAGAATGCCATAAAGAGAA+AGG - chr4_2:56977340-56977359 None:intergenic 30.0%
TTGTTAAACAAGGTGATTCA+AGG - chr4_2:56977533-56977552 None:intergenic 30.0%
! AACAAGAAGAGCTTTTTGTT+TGG + chr4_2:56977634-56977653 Msa0609650:CDS 30.0%
GTTGAACAAAATTTACCTTG+AGG - chr4_2:56977777-56977796 None:intergenic 30.0%
! TATACTCCATGCACAATTTT+GGG + chr4_2:56977872-56977891 Msa0609650:intron 30.0%
AGCAAAAACAGTAGATTGAA+AGG - chr4_2:56977934-56977953 None:intergenic 30.0%
AACAAAACATCTCCCAAAAT+TGG - chr4_2:56978004-56978023 None:intergenic 30.0%
ATATAAAAGTCCAAAATGGG+TGG + chr4_2:56978379-56978398 Msa0609650:intron 30.0%
TATAAAAGTCCAAAATGGGT+GGG + chr4_2:56978380-56978399 Msa0609650:intron 30.0%
!! TTTTCATGTTTGAGGCTAAT+TGG + chr4_2:56978549-56978568 Msa0609650:intron 30.0%
ACTATAGTCAATATGTGTAG+GGG + chr4_2:56978723-56978742 Msa0609650:three_prime_UTR 30.0%
!!! GTTTTTTAGTGTAAAGGTTG+AGG + chr4_2:56978745-56978764 Msa0609650:three_prime_UTR 30.0%
ATAGAATGCCATAAAGAGAA+AGG - chr4_2:56977340-56977359 None:intergenic 30.0%
TTGTTAAACAAGGTGATTCA+AGG - chr4_2:56977533-56977552 None:intergenic 30.0%
! AACAAGAAGAGCTTTTTGTT+TGG + chr4_2:56977634-56977653 Msa0609650:CDS 30.0%
GTTGAACAAAATTTACCTTG+AGG - chr4_2:56977777-56977796 None:intergenic 30.0%
! TATACTCCATGCACAATTTT+GGG + chr4_2:56977872-56977891 Msa0609650:intron 30.0%
AGCAAAAACAGTAGATTGAA+AGG - chr4_2:56977934-56977953 None:intergenic 30.0%
AACAAAACATCTCCCAAAAT+TGG - chr4_2:56978004-56978023 None:intergenic 30.0%
ATATAAAAGTCCAAAATGGG+TGG + chr4_2:56978379-56978398 Msa0609650:intron 30.0%
TATAAAAGTCCAAAATGGGT+GGG + chr4_2:56978380-56978399 Msa0609650:intron 30.0%
!! TTTTCATGTTTGAGGCTAAT+TGG + chr4_2:56978549-56978568 Msa0609650:intron 30.0%
ACTATAGTCAATATGTGTAG+GGG + chr4_2:56978723-56978742 Msa0609650:three_prime_UTR 30.0%
!!! GTTTTTTAGTGTAAAGGTTG+AGG + chr4_2:56978745-56978764 Msa0609650:three_prime_UTR 30.0%
ATGACTATGTTACCCTTCTT+TGG - chr4_2:56977188-56977207 None:intergenic 35.0%
TGTTTGAGAGAGAGATATGA+TGG - chr4_2:56977264-56977283 None:intergenic 35.0%
! GAAGGGTTTAAGGTTAAGTT+GGG - chr4_2:56977311-56977330 None:intergenic 35.0%
AAAGAGAAAGGAACATGGAA+GGG - chr4_2:56977328-56977347 None:intergenic 35.0%
TAAAGAGAAAGGAACATGGA+AGG - chr4_2:56977329-56977348 None:intergenic 35.0%
TCCATGTTCCTTTCTCTTTA+TGG + chr4_2:56977329-56977348 Msa0609650:exon 35.0%
! CTTTTGAACGAAGAAGTTCA+TGG - chr4_2:56977437-56977456 None:intergenic 35.0%
CATGAACTTCTTCGTTCAAA+AGG + chr4_2:56977435-56977454 Msa0609650:CDS 35.0%
TCCTACACTTTCTCAGAAAA+TGG + chr4_2:56977492-56977511 Msa0609650:CDS 35.0%
!! ACCATTTTCTGAGAAAGTGT+AGG - chr4_2:56977496-56977515 None:intergenic 35.0%
!! ATTGTTGATGATCCATCTTC+TGG + chr4_2:56977675-56977694 Msa0609650:CDS 35.0%
AAGAGAATCAAACCAGAAGA+TGG - chr4_2:56977690-56977709 None:intergenic 35.0%
TTACCTTGAGGTTTACAATG+AGG - chr4_2:56977765-56977784 None:intergenic 35.0%
ATGGAGTATAGTTCCAAAAG+GGG - chr4_2:56977862-56977881 None:intergenic 35.0%
CATGGAGTATAGTTCCAAAA+GGG - chr4_2:56977863-56977882 None:intergenic 35.0%
! CTATACTCCATGCACAATTT+TGG + chr4_2:56977871-56977890 Msa0609650:intron 35.0%
TTATGACCCAAAATTGTGCA+TGG - chr4_2:56977881-56977900 None:intergenic 35.0%
GTTGAGTGTGAAATGTTCTT+TGG + chr4_2:56978021-56978040 Msa0609650:intron 35.0%
ATAAAAGTCCAAAATGGGTG+GGG + chr4_2:56978381-56978400 Msa0609650:intron 35.0%
TTGAAGAATCATGTGAGGAA+GGG + chr4_2:56978590-56978609 Msa0609650:CDS 35.0%
!!! GTAGGGGTTTTTTAGTGTAA+AGG + chr4_2:56978739-56978758 Msa0609650:three_prime_UTR 35.0%
! TTTTAGTGTAAAGGTTGAGG+AGG + chr4_2:56978748-56978767 Msa0609650:three_prime_UTR 35.0%
TTTGTTCATTCACCAGCTAT+TGG + chr4_2:56978860-56978879 Msa0609650:three_prime_UTR 35.0%
!! ATTGGTCTTTCTTGCATATC+TGG + chr4_2:56978878-56978897 Msa0609650:three_prime_UTR 35.0%
ATGACTATGTTACCCTTCTT+TGG - chr4_2:56977188-56977207 None:intergenic 35.0%
TGTTTGAGAGAGAGATATGA+TGG - chr4_2:56977264-56977283 None:intergenic 35.0%
! GAAGGGTTTAAGGTTAAGTT+GGG - chr4_2:56977311-56977330 None:intergenic 35.0%
AAAGAGAAAGGAACATGGAA+GGG - chr4_2:56977328-56977347 None:intergenic 35.0%
TAAAGAGAAAGGAACATGGA+AGG - chr4_2:56977329-56977348 None:intergenic 35.0%
TCCATGTTCCTTTCTCTTTA+TGG + chr4_2:56977329-56977348 Msa0609650:exon 35.0%
! CTTTTGAACGAAGAAGTTCA+TGG - chr4_2:56977437-56977456 None:intergenic 35.0%
CATGAACTTCTTCGTTCAAA+AGG + chr4_2:56977435-56977454 Msa0609650:CDS 35.0%
TCCTACACTTTCTCAGAAAA+TGG + chr4_2:56977492-56977511 Msa0609650:CDS 35.0%
!! ACCATTTTCTGAGAAAGTGT+AGG - chr4_2:56977496-56977515 None:intergenic 35.0%
!! ATTGTTGATGATCCATCTTC+TGG + chr4_2:56977675-56977694 Msa0609650:CDS 35.0%
AAGAGAATCAAACCAGAAGA+TGG - chr4_2:56977690-56977709 None:intergenic 35.0%
TTACCTTGAGGTTTACAATG+AGG - chr4_2:56977765-56977784 None:intergenic 35.0%
ATGGAGTATAGTTCCAAAAG+GGG - chr4_2:56977862-56977881 None:intergenic 35.0%
CATGGAGTATAGTTCCAAAA+GGG - chr4_2:56977863-56977882 None:intergenic 35.0%
! CTATACTCCATGCACAATTT+TGG + chr4_2:56977871-56977890 Msa0609650:intron 35.0%
TTATGACCCAAAATTGTGCA+TGG - chr4_2:56977881-56977900 None:intergenic 35.0%
GTTGAGTGTGAAATGTTCTT+TGG + chr4_2:56978021-56978040 Msa0609650:intron 35.0%
ATAAAAGTCCAAAATGGGTG+GGG + chr4_2:56978381-56978400 Msa0609650:intron 35.0%
TTGAAGAATCATGTGAGGAA+GGG + chr4_2:56978590-56978609 Msa0609650:CDS 35.0%
!!! GTAGGGGTTTTTTAGTGTAA+AGG + chr4_2:56978739-56978758 Msa0609650:three_prime_UTR 35.0%
! TTTTAGTGTAAAGGTTGAGG+AGG + chr4_2:56978748-56978767 Msa0609650:three_prime_UTR 35.0%
TTTGTTCATTCACCAGCTAT+TGG + chr4_2:56978860-56978879 Msa0609650:three_prime_UTR 35.0%
!! ATTGGTCTTTCTTGCATATC+TGG + chr4_2:56978878-56978897 Msa0609650:three_prime_UTR 35.0%
GTGACTTTGGTTGTGTTTGT+GGG - chr4_2:56977162-56977181 None:intergenic 40.0%
CAAAGTCACCAACCAAAGAA+GGG + chr4_2:56977173-56977192 Msa0609650:five_prime_UTR 40.0%
CTATGTTACCCTTCTTTGGT+TGG - chr4_2:56977184-56977203 None:intergenic 40.0%
! GGAAGGGTTTAAGGTTAAGT+TGG - chr4_2:56977312-56977331 None:intergenic 40.0%
AAGGAACATGGAAGGGTTTA+AGG - chr4_2:56977321-56977340 None:intergenic 40.0%
GCCATAAAGAGAAAGGAACA+TGG - chr4_2:56977333-56977352 None:intergenic 40.0%
AGAAGAGGAGAGAGAAAGTT+GGG - chr4_2:56977409-56977428 None:intergenic 40.0%
AAGAAGAGGAGAGAGAAAGT+TGG - chr4_2:56977410-56977429 None:intergenic 40.0%
AAGTTCATGGATGGAAGAAG+AGG - chr4_2:56977424-56977443 None:intergenic 40.0%
TGAACGAAGAAGTTCATGGA+TGG - chr4_2:56977433-56977452 None:intergenic 40.0%
GTGTAGGACTTTATCTCTTC+TGG - chr4_2:56977480-56977499 None:intergenic 40.0%
ATCACTGCTAATCTCACCTA+TGG + chr4_2:56977585-56977604 Msa0609650:CDS 40.0%
AACACCAATGAGACTTCCAT+AGG - chr4_2:56977604-56977623 None:intergenic 40.0%
GGTTTGATTCTCTTCGACAT+TGG + chr4_2:56977696-56977715 Msa0609650:CDS 40.0%
GCATGGAGTATAGTTCCAAA+AGG - chr4_2:56977864-56977883 None:intergenic 40.0%
! TAAATTAGCCCCACCCATTT+TGG - chr4_2:56978392-56978411 None:intergenic 40.0%
! GTGAGTTGAAGAATCATGTG+AGG + chr4_2:56978585-56978604 Msa0609650:CDS 40.0%
GTTGAAGAATCATGTGAGGA+AGG + chr4_2:56978589-56978608 Msa0609650:CDS 40.0%
TGAAGAATCATGTGAGGAAG+GGG + chr4_2:56978591-56978610 Msa0609650:CDS 40.0%
! AAGATGCCAACTCAGTTTTC+AGG + chr4_2:56978641-56978660 Msa0609650:three_prime_UTR 40.0%
TTGAAGCCTGAAAACTGAGT+TGG - chr4_2:56978650-56978669 None:intergenic 40.0%
AGAACATGTAAAGATGTGGG+TGG + chr4_2:56978781-56978800 Msa0609650:three_prime_UTR 40.0%
GAACATGTAAAGATGTGGGT+GGG + chr4_2:56978782-56978801 Msa0609650:three_prime_UTR 40.0%
AACATGTAAAGATGTGGGTG+GGG + chr4_2:56978783-56978802 Msa0609650:three_prime_UTR 40.0%
ATGCAAGAAAGACCAATAGC+TGG - chr4_2:56978875-56978894 None:intergenic 40.0%
GTGACTTTGGTTGTGTTTGT+GGG - chr4_2:56977162-56977181 None:intergenic 40.0%
CAAAGTCACCAACCAAAGAA+GGG + chr4_2:56977173-56977192 Msa0609650:five_prime_UTR 40.0%
CTATGTTACCCTTCTTTGGT+TGG - chr4_2:56977184-56977203 None:intergenic 40.0%
! GGAAGGGTTTAAGGTTAAGT+TGG - chr4_2:56977312-56977331 None:intergenic 40.0%
AAGGAACATGGAAGGGTTTA+AGG - chr4_2:56977321-56977340 None:intergenic 40.0%
GCCATAAAGAGAAAGGAACA+TGG - chr4_2:56977333-56977352 None:intergenic 40.0%
AGAAGAGGAGAGAGAAAGTT+GGG - chr4_2:56977409-56977428 None:intergenic 40.0%
AAGAAGAGGAGAGAGAAAGT+TGG - chr4_2:56977410-56977429 None:intergenic 40.0%
AAGTTCATGGATGGAAGAAG+AGG - chr4_2:56977424-56977443 None:intergenic 40.0%
TGAACGAAGAAGTTCATGGA+TGG - chr4_2:56977433-56977452 None:intergenic 40.0%
GTGTAGGACTTTATCTCTTC+TGG - chr4_2:56977480-56977499 None:intergenic 40.0%
ATCACTGCTAATCTCACCTA+TGG + chr4_2:56977585-56977604 Msa0609650:CDS 40.0%
AACACCAATGAGACTTCCAT+AGG - chr4_2:56977604-56977623 None:intergenic 40.0%
GGTTTGATTCTCTTCGACAT+TGG + chr4_2:56977696-56977715 Msa0609650:CDS 40.0%
GCATGGAGTATAGTTCCAAA+AGG - chr4_2:56977864-56977883 None:intergenic 40.0%
! TAAATTAGCCCCACCCATTT+TGG - chr4_2:56978392-56978411 None:intergenic 40.0%
! GTGAGTTGAAGAATCATGTG+AGG + chr4_2:56978585-56978604 Msa0609650:CDS 40.0%
GTTGAAGAATCATGTGAGGA+AGG + chr4_2:56978589-56978608 Msa0609650:CDS 40.0%
TGAAGAATCATGTGAGGAAG+GGG + chr4_2:56978591-56978610 Msa0609650:CDS 40.0%
! AAGATGCCAACTCAGTTTTC+AGG + chr4_2:56978641-56978660 Msa0609650:three_prime_UTR 40.0%
TTGAAGCCTGAAAACTGAGT+TGG - chr4_2:56978650-56978669 None:intergenic 40.0%
AGAACATGTAAAGATGTGGG+TGG + chr4_2:56978781-56978800 Msa0609650:three_prime_UTR 40.0%
GAACATGTAAAGATGTGGGT+GGG + chr4_2:56978782-56978801 Msa0609650:three_prime_UTR 40.0%
AACATGTAAAGATGTGGGTG+GGG + chr4_2:56978783-56978802 Msa0609650:three_prime_UTR 40.0%
ATGCAAGAAAGACCAATAGC+TGG - chr4_2:56978875-56978894 None:intergenic 40.0%
GGTGACTTTGGTTGTGTTTG+TGG - chr4_2:56977163-56977182 None:intergenic 45.0%
!! CCTTCTTTGGTTGGTGACTT+TGG - chr4_2:56977175-56977194 None:intergenic 45.0%
CCAAAGTCACCAACCAAAGA+AGG + chr4_2:56977172-56977191 Msa0609650:five_prime_UTR 45.0%
GAAGAGGAGAGAGAAAGTTG+GGG - chr4_2:56977408-56977427 None:intergenic 45.0%
CTCACCTATGGAAGTCTCAT+TGG + chr4_2:56977597-56977616 Msa0609650:CDS 45.0%
!! GGAAGTCTCATTGGTGTTGA+AGG + chr4_2:56977606-56977625 Msa0609650:CDS 45.0%
CCTTGAGGTTTACAATGAGG+TGG - chr4_2:56977762-56977781 None:intergenic 45.0%
CCACCTCATTGTAAACCTCA+AGG + chr4_2:56977759-56977778 Msa0609650:CDS 45.0%
! AGATCTGCTACTACCCCTTT+TGG + chr4_2:56977846-56977865 Msa0609650:intron 45.0%
! GCTAATTGGTTGAGTTGTGC+AGG + chr4_2:56978563-56978582 Msa0609650:intron 45.0%
GGTGACTTTGGTTGTGTTTG+TGG - chr4_2:56977163-56977182 None:intergenic 45.0%
!! CCTTCTTTGGTTGGTGACTT+TGG - chr4_2:56977175-56977194 None:intergenic 45.0%
CCAAAGTCACCAACCAAAGA+AGG + chr4_2:56977172-56977191 Msa0609650:five_prime_UTR 45.0%
GAAGAGGAGAGAGAAAGTTG+GGG - chr4_2:56977408-56977427 None:intergenic 45.0%
CTCACCTATGGAAGTCTCAT+TGG + chr4_2:56977597-56977616 Msa0609650:CDS 45.0%
!! GGAAGTCTCATTGGTGTTGA+AGG + chr4_2:56977606-56977625 Msa0609650:CDS 45.0%
CCTTGAGGTTTACAATGAGG+TGG - chr4_2:56977762-56977781 None:intergenic 45.0%
CCACCTCATTGTAAACCTCA+AGG + chr4_2:56977759-56977778 Msa0609650:CDS 45.0%
! AGATCTGCTACTACCCCTTT+TGG + chr4_2:56977846-56977865 Msa0609650:intron 45.0%
! GCTAATTGGTTGAGTTGTGC+AGG + chr4_2:56978563-56978582 Msa0609650:intron 45.0%
AAAGTTGGGGAGAGAGGAGA+AGG - chr4_2:56977395-56977414 None:intergenic 50.0%
AGAGAGAAAGTTGGGGAGAG+AGG - chr4_2:56977401-56977420 None:intergenic 50.0%
CGTTCAAAAGGCTTACCAGC+TGG + chr4_2:56977447-56977466 Msa0609650:CDS 50.0%
TCTTCTGGTAAGAGACCAGC+TGG - chr4_2:56977465-56977484 None:intergenic 50.0%
AATCATGTGAGGAAGGGGAG+AGG + chr4_2:56978596-56978615 Msa0609650:CDS 50.0%
AAAGTTGGGGAGAGAGGAGA+AGG - chr4_2:56977395-56977414 None:intergenic 50.0%
AGAGAGAAAGTTGGGGAGAG+AGG - chr4_2:56977401-56977420 None:intergenic 50.0%
CGTTCAAAAGGCTTACCAGC+TGG + chr4_2:56977447-56977466 Msa0609650:CDS 50.0%
TCTTCTGGTAAGAGACCAGC+TGG - chr4_2:56977465-56977484 None:intergenic 50.0%
AATCATGTGAGGAAGGGGAG+AGG + chr4_2:56978596-56978615 Msa0609650:CDS 50.0%
AGAGGAGAAGGAGGAGAGAG+AGG - chr4_2:56977383-56977402 None:intergenic 55.0%
GAGGAAGGGGAGAGGATTTG+AGG + chr4_2:56978604-56978623 Msa0609650:CDS 55.0%
! GGAGAGGATTTGAGGCAGTG+AGG + chr4_2:56978612-56978631 Msa0609650:CDS 55.0%
AGAGGAGAAGGAGGAGAGAG+AGG - chr4_2:56977383-56977402 None:intergenic 55.0%
GAGGAAGGGGAGAGGATTTG+AGG + chr4_2:56978604-56978623 Msa0609650:CDS 55.0%
! GGAGAGGATTTGAGGCAGTG+AGG + chr4_2:56978612-56978631 Msa0609650:CDS 55.0%
GTTGGGGAGAGAGGAGAAGG+AGG - chr4_2:56977392-56977411 None:intergenic 60.0%
GTTGGGGAGAGAGGAGAAGG+AGG - chr4_2:56977392-56977411 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 56977151 56979055 56977151 ID=Msa0609650;Name=Msa0609650
chr4_2 mRNA 56977151 56979055 56977151 ID=Msa0609650-mRNA-1;Parent=Msa0609650;Name=Msa0609650-mRNA-1;_AED=0.02;_eAED=0.02;_QI=197|1|1|1|1|1|2|418|161
chr4_2 exon 56977151 56977780 56977151 ID=Msa0609650-mRNA-1:exon:14763;Parent=Msa0609650-mRNA-1
chr4_2 exon 56978585 56979055 56978585 ID=Msa0609650-mRNA-1:exon:14764;Parent=Msa0609650-mRNA-1
chr4_2 five_prime_UTR 56977151 56977347 56977151 ID=Msa0609650-mRNA-1:five_prime_utr;Parent=Msa0609650-mRNA-1
chr4_2 CDS 56977348 56977780 56977348 ID=Msa0609650-mRNA-1:cds;Parent=Msa0609650-mRNA-1
chr4_2 CDS 56978585 56978637 56978585 ID=Msa0609650-mRNA-1:cds;Parent=Msa0609650-mRNA-1
chr4_2 three_prime_UTR 56978638 56979055 56978638 ID=Msa0609650-mRNA-1:three_prime_utr;Parent=Msa0609650-mRNA-1
Gene Sequence

>Msa0609650

ATGGCATTCTATAACACTCTTCTCTCTTTCTTCCTCTCTCTCCTCCTTCTCCTCTCTCCCCAACTTTCTCTCTCCTCTTCTTCCATCCATGAACTTCTTCGTTCAAAAGGCTTACCAGCTGGTCTCTTACCAGAAGAGATAAAGTCCTACACTTTCTCAGAAAATGGTCACTTAGAAGTGTTCCTTGAATCACCTTGTTTAACAAAGTATGAAAACAGAGTCTACTTTGAACAAGTTATCACTGCTAATCTCACCTATGGAAGTCTCATTGGTGTTGAAGGTTTGCAACAAGAAGAGCTTTTTGTTTGGTTACCTGTTAAAGATATCATTGTTGATGATCCATCTTCTGGTTTGATTCTCTTCGACATTGGTCTTGCTTATAAACAACTCTCTTTCTCTCTCTTTGAAGTTCCACCTCATTGTAAACCTCAAGGTGAGTTGAAGAATCATGTGAGGAAGGGGAGAGGATTTGAGGCAGTGAGGTAG
Protein sequence

>Msa0609650

MAFYNTLLSFFLSLLLLLSPQLSLSSSSIHELLRSKGLPAGLLPEEIKSYTFSENGHLEVFLESPCLTKYENRVYFEQVITANLTYGSLIGVEGLQQEELFVWLPVKDIIVDDPSSGLILFDIGLAYKQLSFSLFEVPPHCKPQGELKNHVRKGRGFEAVR*