AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0692120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0692120 MtrunA17_Chr4g0042211 98.758 161 2 0 1 161 1 161 2.77e-113 318
Msa0692120 MtrunA17_Chr2g0280591 63.971 136 48 1 27 161 26 161 8.04e-58 177
Msa0692120 MtrunA17_Chr4g0048861 48.921 139 70 1 5 142 6 144 1.82e-44 143
Msa0692120 MtrunA17_Chr4g0048881 50.376 133 66 0 10 142 12 144 5.25e-44 143
Msa0692120 MtrunA17_Chr4g0048871 49.286 140 69 2 5 142 6 145 1.84e-43 141
Msa0692120 MtrunA17_Chr4g0031861 55.000 120 53 1 27 145 29 148 3.18e-42 138
Msa0692120 MtrunA17_Chr7g0249501 47.934 121 62 1 25 144 37 157 2.46e-37 126
Msa0692120 MtrunA17_Chr7g0234601 44.248 113 63 0 29 141 36 148 7.75e-34 117
Msa0692120 MtrunA17_Chr8g0378501 43.103 116 64 2 27 141 29 143 9.68e-29 103
Msa0692120 MtrunA17_Chr8g0378491 35.652 115 74 0 28 142 29 143 2.96e-24 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0692120 AT5G54530.1 62.121 132 50 0 28 159 28 159 1.70e-61 187
Msa0692120 AT1G61667.1 57.576 132 55 1 25 155 18 149 1.40e-52 164
Msa0692120 AT1G61667.2 57.576 132 55 1 25 155 54 185 4.35e-52 164
Msa0692120 AT3G07470.2 40.876 137 77 2 11 143 10 146 1.07e-32 114
Msa0692120 AT3G07470.3 40.876 137 77 2 11 143 10 146 1.31e-32 114
Msa0692120 AT3G07470.1 40.876 137 77 2 11 143 10 146 1.93e-32 114
Msa0692120 AT3G07460.1 40.876 137 79 2 6 141 8 143 4.81e-32 113
Msa0692120 AT3G07460.2 40.876 137 79 2 6 141 8 143 7.26e-31 112
Msa0692120 AT5G16380.1 43.796 137 72 3 10 141 8 144 5.21e-29 105
Msa0692120 AT1G55265.1 39.844 128 75 2 23 149 48 174 8.20e-28 102
Msa0692120 AT5G19860.1 34.711 121 79 0 25 145 32 152 1.18e-22 89.0
Msa0692120 AT1G02813.1 28.058 139 92 4 8 141 1 136 4.83e-11 58.2

Find 59 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATGTTCCTTTCTCTTTA+TGG 0.022892 4_4:-60472211 Msa0692120:exon
AAGGAACATGGAAGGGTTTA+AGG 0.156283 4_4:+60472222 None:intergenic
ATGACTATGTTACCCTTCTT+TGG 0.279545 4_4:+60472355 None:intergenic
GTGTAGGACTTTATCTCTTC+TGG 0.280118 4_4:+60472063 None:intergenic
TTGTCATGTTTATATACTTT+TGG 0.301741 4_4:-60470856 Msa0692120:three_prime_UTR
AGAAGAGGAGAGAGAAAGTT+GGG 0.313243 4_4:+60472134 None:intergenic
TCCTACACTTTCTCAGAAAA+TGG 0.342412 4_4:-60472048 Msa0692120:CDS
TTGTGTTTGTGGGTCTTTGA+TGG 0.344987 4_4:+60472391 None:intergenic
ATTGTTGATGATCCATCTTC+TGG 0.353815 4_4:-60471865 Msa0692120:CDS
GGAAGGGTTTAAGGTTAAGT+TGG 0.355475 4_4:+60472231 None:intergenic
CATGAACTTCTTCGTTCAAA+AGG 0.359352 4_4:-60472105 Msa0692120:CDS
CCTTCTTTGGTTGGTGACTT+TGG 0.373102 4_4:+60472368 None:intergenic
GGTTTGATTCTCTTTGACAT+TGG 0.373655 4_4:-60471844 Msa0692120:CDS
GTGACTTTGGTTGTGTTTGT+GGG 0.384133 4_4:+60472381 None:intergenic
GGTGACTTTGGTTGTGTTTG+TGG 0.384303 4_4:+60472380 None:intergenic
GGAAGTCTCATTGGTGTTGA+AGG 0.392093 4_4:-60471934 Msa0692120:CDS
GAGGAAGGAGAGAGGATTTG+AGG 0.414030 4_4:-60470937 Msa0692120:CDS
AGAGGAGAAGGAGGAGAGAG+AGG 0.416690 4_4:+60472160 None:intergenic
TCAACAATGATATCTTTAAC+AGG 0.417805 4_4:+60471880 None:intergenic
AGAGAGAAAGTTGGGGAGAG+AGG 0.420739 4_4:+60472142 None:intergenic
TTTGTTCATTCACCAGCTAT+TGG 0.424105 4_4:-60470681 Msa0692120:three_prime_UTR
CTCACCTATGGAAGTCTCAT+TGG 0.430019 4_4:-60471943 Msa0692120:CDS
AAAGTTGGGGAGAGAGGAGA+AGG 0.432500 4_4:+60472148 None:intergenic
GAAGGGTTTAAGGTTAAGTT+GGG 0.433298 4_4:+60472232 None:intergenic
AAGAAGAGGAGAGAGAAAGT+TGG 0.438219 4_4:+60472133 None:intergenic
CCAAAGTCACCAACCAAAGA+AGG 0.456555 4_4:-60472368 Msa0692120:five_prime_UTR
GTTGGGGAGAGAGGAGAAGG+AGG 0.460848 4_4:+60472151 None:intergenic
AATCATGTGAGGAAGGAGAG+AGG 0.462940 4_4:-60470945 Msa0692120:CDS
CTATGTTACCCTTCTTTGGT+TGG 0.463103 4_4:+60472359 None:intergenic
AACTATAGTCAATATGTGTA+GGG 0.474579 4_4:-60470819 Msa0692120:three_prime_UTR
GTTGAAGAATCATGTGAGGA+AGG 0.477726 4_4:-60470952 Msa0692120:CDS
TTGTTAAACAAGGTGATTCA+AGG 0.482786 4_4:+60472010 None:intergenic
AAGAGAATCAAACCAGAAGA+TGG 0.489401 4_4:+60471853 None:intergenic
AAACTATAGTCAATATGTGT+AGG 0.490066 4_4:-60470820 Msa0692120:three_prime_UTR
TGTTTGAGAGAGAGATATGA+TGG 0.499117 4_4:+60472279 None:intergenic
ATAGAATGCCATAAAGAGAA+AGG 0.502220 4_4:+60472203 None:intergenic
AAGTTCATGGATGGAAGAAG+AGG 0.514641 4_4:+60472119 None:intergenic
AACATGTAAAGATGTGGGTG+GGG 0.517641 4_4:-60470758 Msa0692120:three_prime_UTR
TCTTCTGGTAAGAGACCAGC+TGG 0.524230 4_4:+60472078 None:intergenic
CGTTCAAAAGGCTTACCAGC+TGG 0.528273 4_4:-60472093 Msa0692120:CDS
GAACATGTAAAGATGTGGGT+GGG 0.529395 4_4:-60470759 Msa0692120:three_prime_UTR
ATCACTGCTAATCTCACCTA+TGG 0.534134 4_4:-60471955 Msa0692120:CDS
AGAGAGGATTTGAGGCAGTG+AGG 0.544712 4_4:-60470929 Msa0692120:CDS
TTGAAGCCTGAAAACTGAGT+TGG 0.544762 4_4:+60470894 None:intergenic
CCTTGAGGTTTACAATGAGG+TGG 0.551693 4_4:+60471781 None:intergenic
TGAACGAAGAAGTTCATGGA+TGG 0.572953 4_4:+60472110 None:intergenic
GAAGAGGAGAGAGAAAGTTG+GGG 0.580735 4_4:+60472135 None:intergenic
ATTAAGAACATGTAAAGATG+TGG 0.585266 4_4:-60470764 Msa0692120:three_prime_UTR
AAAGAGAAAGGAACATGGAA+GGG 0.586134 4_4:+60472215 None:intergenic
TAAAGAGAAAGGAACATGGA+AGG 0.596020 4_4:+60472214 None:intergenic
CCACCTCATTGTAAACCTCA+AGG 0.602779 4_4:-60471781 Msa0692120:intron
CAAAGTCACCAACCAAAGAA+GGG 0.606616 4_4:-60472367 Msa0692120:five_prime_UTR
TTACCTTGAGGTTTACAATG+AGG 0.607565 4_4:+60471778 None:intergenic
AGAACATGTAAAGATGTGGG+TGG 0.621050 4_4:-60470760 Msa0692120:three_prime_UTR
GCCATAAAGAGAAAGGAACA+TGG 0.627091 4_4:+60472210 None:intergenic
TTAAGAACATGTAAAGATGT+GGG 0.632160 4_4:-60470763 Msa0692120:three_prime_UTR
ACTATAGTCAATATGTGTAG+GGG 0.656085 4_4:-60470818 Msa0692120:three_prime_UTR
AACACCAATGAGACTTCCAT+AGG 0.677885 4_4:+60471939 None:intergenic
GTGAGTTGAAGAATCATGTG+AGG 0.690883 4_4:-60470956 Msa0692120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTCTTTCCAATTT+TGG - chr4_4:60471344-60471363 Msa0692120:intron 15.0%
!!! TTTTTTTTCTTTCCAATTTT+GGG - chr4_4:60471345-60471364 Msa0692120:intron 15.0%
!!! ATTTGCTTTTAATTATACAA+AGG + chr4_4:60471627-60471646 None:intergenic 15.0%
!! ATATATATAAAAGTCCAAAA+TGG - chr4_4:60471731-60471750 Msa0692120:intron 15.0%
!! TATATATAAAAGTCCAAAAT+GGG - chr4_4:60471732-60471751 Msa0692120:intron 15.0%
!!! TCTTTTTTACTTTTTGTTAA+AGG - chr4_4:60472267-60472286 Msa0692120:five_prime_UTR 15.0%
!!! TTTTTTTTTCTTTCCAATTT+TGG - chr4_4:60471344-60471363 Msa0692120:intron 15.0%
!!! TTTTTTTTCTTTCCAATTTT+GGG - chr4_4:60471345-60471364 Msa0692120:intron 15.0%
!!! ATTTGCTTTTAATTATACAA+AGG + chr4_4:60471627-60471646 None:intergenic 15.0%
!! ATATATATAAAAGTCCAAAA+TGG - chr4_4:60471731-60471750 Msa0692120:intron 15.0%
!! TATATATAAAAGTCCAAAAT+GGG - chr4_4:60471732-60471751 Msa0692120:intron 15.0%
!!! TCTTTTTTACTTTTTGTTAA+AGG - chr4_4:60472267-60472286 Msa0692120:five_prime_UTR 15.0%
!!! TTTTCATACTTTGTTAAACA+AGG + chr4_4:60470900-60470919 None:intergenic 20.0%
!!! TTGGAATTTTGTATTAATTG+AGG - chr4_4:60471396-60471415 Msa0692120:intron 20.0%
!!! TTCAATTTTTTTCATGTTTG+AGG - chr4_4:60471897-60471916 Msa0692120:CDS 20.0%
!!! TTGTCATGTTTATATACTTT+TGG - chr4_4:60472041-60472060 Msa0692120:CDS 20.0%
!!! TTTTCATACTTTGTTAAACA+AGG + chr4_4:60470900-60470919 None:intergenic 20.0%
!!! TTGGAATTTTGTATTAATTG+AGG - chr4_4:60471396-60471415 Msa0692120:intron 20.0%
!!! TTCAATTTTTTTCATGTTTG+AGG - chr4_4:60471897-60471916 Msa0692120:CDS 20.0%
!!! TTGTCATGTTTATATACTTT+TGG - chr4_4:60472041-60472060 Msa0692120:CDS 20.0%
! TCAACAATGATATCTTTAAC+AGG + chr4_4:60471020-60471039 None:intergenic 25.0%
!!! TCTAACTTTGTTATTGTCTT+TGG + chr4_4:60471573-60471592 None:intergenic 25.0%
! AATAACAAAGTTAGAAGAGA+AGG - chr4_4:60471578-60471597 Msa0692120:intron 25.0%
!! TGTCATTATTTTCAACTTTG+CGG - chr4_4:60471824-60471843 Msa0692120:CDS 25.0%
! TATGTGTTGAAAGATGAAAA+TGG - chr4_4:60471854-60471873 Msa0692120:CDS 25.0%
! AAACTATAGTCAATATGTGT+AGG - chr4_4:60472077-60472096 Msa0692120:CDS 25.0%
! AACTATAGTCAATATGTGTA+GGG - chr4_4:60472078-60472097 Msa0692120:CDS 25.0%
! ATTAAGAACATGTAAAGATG+TGG - chr4_4:60472133-60472152 Msa0692120:CDS 25.0%
! TTAAGAACATGTAAAGATGT+GGG - chr4_4:60472134-60472153 Msa0692120:CDS 25.0%
! TCAACAATGATATCTTTAAC+AGG + chr4_4:60471020-60471039 None:intergenic 25.0%
!!! TCTAACTTTGTTATTGTCTT+TGG + chr4_4:60471573-60471592 None:intergenic 25.0%
! AATAACAAAGTTAGAAGAGA+AGG - chr4_4:60471578-60471597 Msa0692120:intron 25.0%
!! TGTCATTATTTTCAACTTTG+CGG - chr4_4:60471824-60471843 Msa0692120:CDS 25.0%
! TATGTGTTGAAAGATGAAAA+TGG - chr4_4:60471854-60471873 Msa0692120:CDS 25.0%
! AAACTATAGTCAATATGTGT+AGG - chr4_4:60472077-60472096 Msa0692120:CDS 25.0%
! AACTATAGTCAATATGTGTA+GGG - chr4_4:60472078-60472097 Msa0692120:CDS 25.0%
! ATTAAGAACATGTAAAGATG+TGG - chr4_4:60472133-60472152 Msa0692120:CDS 25.0%
! TTAAGAACATGTAAAGATGT+GGG - chr4_4:60472134-60472153 Msa0692120:CDS 25.0%
ATAGAATGCCATAAAGAGAA+AGG + chr4_4:60470697-60470716 None:intergenic 30.0%
TTGTTAAACAAGGTGATTCA+AGG + chr4_4:60470890-60470909 None:intergenic 30.0%
! AACAAGAAGAGCTTTTTGTT+TGG - chr4_4:60470991-60471010 Msa0692120:intron 30.0%
GTTGAACAAAATTTACCTTG+AGG + chr4_4:60471134-60471153 None:intergenic 30.0%
! TATACTCCATGCACAATTTT+GGG - chr4_4:60471229-60471248 Msa0692120:intron 30.0%
AGCAAAAACAGTAGATTGAA+AGG + chr4_4:60471291-60471310 None:intergenic 30.0%
AACAAAACATCTCCCAAAAT+TGG + chr4_4:60471360-60471379 None:intergenic 30.0%
AGAAGGAAAAAACAATTGAG+AGG - chr4_4:60471595-60471614 Msa0692120:intron 30.0%
ATATAAAAGTCCAAAATGGG+TGG - chr4_4:60471735-60471754 Msa0692120:intron 30.0%
TATAAAAGTCCAAAATGGGT+GGG - chr4_4:60471736-60471755 Msa0692120:intron 30.0%
!! TTTTCATGTTTGAGGCTAAT+TGG - chr4_4:60471905-60471924 Msa0692120:CDS 30.0%
ACTATAGTCAATATGTGTAG+GGG - chr4_4:60472079-60472098 Msa0692120:CDS 30.0%
!!! GTTTTTTAGTGTAAAGGTTG+AGG - chr4_4:60472101-60472120 Msa0692120:CDS 30.0%
!!! ATTGGTCTTTTTTGCATATC+TGG - chr4_4:60472234-60472253 Msa0692120:five_prime_UTR 30.0%
ATAGAATGCCATAAAGAGAA+AGG + chr4_4:60470697-60470716 None:intergenic 30.0%
TTGTTAAACAAGGTGATTCA+AGG + chr4_4:60470890-60470909 None:intergenic 30.0%
! AACAAGAAGAGCTTTTTGTT+TGG - chr4_4:60470991-60471010 Msa0692120:intron 30.0%
GTTGAACAAAATTTACCTTG+AGG + chr4_4:60471134-60471153 None:intergenic 30.0%
! TATACTCCATGCACAATTTT+GGG - chr4_4:60471229-60471248 Msa0692120:intron 30.0%
AGCAAAAACAGTAGATTGAA+AGG + chr4_4:60471291-60471310 None:intergenic 30.0%
AACAAAACATCTCCCAAAAT+TGG + chr4_4:60471360-60471379 None:intergenic 30.0%
AGAAGGAAAAAACAATTGAG+AGG - chr4_4:60471595-60471614 Msa0692120:intron 30.0%
ATATAAAAGTCCAAAATGGG+TGG - chr4_4:60471735-60471754 Msa0692120:intron 30.0%
TATAAAAGTCCAAAATGGGT+GGG - chr4_4:60471736-60471755 Msa0692120:intron 30.0%
!! TTTTCATGTTTGAGGCTAAT+TGG - chr4_4:60471905-60471924 Msa0692120:CDS 30.0%
ACTATAGTCAATATGTGTAG+GGG - chr4_4:60472079-60472098 Msa0692120:CDS 30.0%
!!! GTTTTTTAGTGTAAAGGTTG+AGG - chr4_4:60472101-60472120 Msa0692120:CDS 30.0%
!!! ATTGGTCTTTTTTGCATATC+TGG - chr4_4:60472234-60472253 Msa0692120:five_prime_UTR 30.0%
ATGACTATGTTACCCTTCTT+TGG + chr4_4:60470545-60470564 None:intergenic 35.0%
TGTTTGAGAGAGAGATATGA+TGG + chr4_4:60470621-60470640 None:intergenic 35.0%
! GAAGGGTTTAAGGTTAAGTT+GGG + chr4_4:60470668-60470687 None:intergenic 35.0%
AAAGAGAAAGGAACATGGAA+GGG + chr4_4:60470685-60470704 None:intergenic 35.0%
TAAAGAGAAAGGAACATGGA+AGG + chr4_4:60470686-60470705 None:intergenic 35.0%
TCCATGTTCCTTTCTCTTTA+TGG - chr4_4:60470686-60470705 Msa0692120:three_prime_UTR 35.0%
! CTTTTGAACGAAGAAGTTCA+TGG + chr4_4:60470794-60470813 None:intergenic 35.0%
CATGAACTTCTTCGTTCAAA+AGG - chr4_4:60470792-60470811 Msa0692120:three_prime_UTR 35.0%
TCCTACACTTTCTCAGAAAA+TGG - chr4_4:60470849-60470868 Msa0692120:three_prime_UTR 35.0%
!! ACCATTTTCTGAGAAAGTGT+AGG + chr4_4:60470853-60470872 None:intergenic 35.0%
!! ATTGTTGATGATCCATCTTC+TGG - chr4_4:60471032-60471051 Msa0692120:intron 35.0%
AAGAGAATCAAACCAGAAGA+TGG + chr4_4:60471047-60471066 None:intergenic 35.0%
GGTTTGATTCTCTTTGACAT+TGG - chr4_4:60471053-60471072 Msa0692120:intron 35.0%
TTACCTTGAGGTTTACAATG+AGG + chr4_4:60471122-60471141 None:intergenic 35.0%
ATGGAGTATAGTTCCAAAAG+GGG + chr4_4:60471219-60471238 None:intergenic 35.0%
CATGGAGTATAGTTCCAAAA+GGG + chr4_4:60471220-60471239 None:intergenic 35.0%
! CTATACTCCATGCACAATTT+TGG - chr4_4:60471228-60471247 Msa0692120:intron 35.0%
TTATGACCCAAAATTGTGCA+TGG + chr4_4:60471238-60471257 None:intergenic 35.0%
GTTGAGTGTGAAATGTTCTT+TGG - chr4_4:60471377-60471396 Msa0692120:intron 35.0%
ATAAAAGTCCAAAATGGGTG+GGG - chr4_4:60471737-60471756 Msa0692120:intron 35.0%
!!! GTAGGGGTTTTTTAGTGTAA+AGG - chr4_4:60472095-60472114 Msa0692120:CDS 35.0%
! TTTTAGTGTAAAGGTTGAGG+AGG - chr4_4:60472104-60472123 Msa0692120:CDS 35.0%
TTTGTTCATTCACCAGCTAT+TGG - chr4_4:60472216-60472235 Msa0692120:five_prime_UTR 35.0%
ATGCAAAAAAGACCAATAGC+TGG + chr4_4:60472231-60472250 None:intergenic 35.0%
ATGACTATGTTACCCTTCTT+TGG + chr4_4:60470545-60470564 None:intergenic 35.0%
TGTTTGAGAGAGAGATATGA+TGG + chr4_4:60470621-60470640 None:intergenic 35.0%
! GAAGGGTTTAAGGTTAAGTT+GGG + chr4_4:60470668-60470687 None:intergenic 35.0%
AAAGAGAAAGGAACATGGAA+GGG + chr4_4:60470685-60470704 None:intergenic 35.0%
TAAAGAGAAAGGAACATGGA+AGG + chr4_4:60470686-60470705 None:intergenic 35.0%
TCCATGTTCCTTTCTCTTTA+TGG - chr4_4:60470686-60470705 Msa0692120:three_prime_UTR 35.0%
! CTTTTGAACGAAGAAGTTCA+TGG + chr4_4:60470794-60470813 None:intergenic 35.0%
CATGAACTTCTTCGTTCAAA+AGG - chr4_4:60470792-60470811 Msa0692120:three_prime_UTR 35.0%
TCCTACACTTTCTCAGAAAA+TGG - chr4_4:60470849-60470868 Msa0692120:three_prime_UTR 35.0%
!! ACCATTTTCTGAGAAAGTGT+AGG + chr4_4:60470853-60470872 None:intergenic 35.0%
!! ATTGTTGATGATCCATCTTC+TGG - chr4_4:60471032-60471051 Msa0692120:intron 35.0%
AAGAGAATCAAACCAGAAGA+TGG + chr4_4:60471047-60471066 None:intergenic 35.0%
GGTTTGATTCTCTTTGACAT+TGG - chr4_4:60471053-60471072 Msa0692120:intron 35.0%
TTACCTTGAGGTTTACAATG+AGG + chr4_4:60471122-60471141 None:intergenic 35.0%
ATGGAGTATAGTTCCAAAAG+GGG + chr4_4:60471219-60471238 None:intergenic 35.0%
CATGGAGTATAGTTCCAAAA+GGG + chr4_4:60471220-60471239 None:intergenic 35.0%
! CTATACTCCATGCACAATTT+TGG - chr4_4:60471228-60471247 Msa0692120:intron 35.0%
TTATGACCCAAAATTGTGCA+TGG + chr4_4:60471238-60471257 None:intergenic 35.0%
GTTGAGTGTGAAATGTTCTT+TGG - chr4_4:60471377-60471396 Msa0692120:intron 35.0%
ATAAAAGTCCAAAATGGGTG+GGG - chr4_4:60471737-60471756 Msa0692120:intron 35.0%
!!! GTAGGGGTTTTTTAGTGTAA+AGG - chr4_4:60472095-60472114 Msa0692120:CDS 35.0%
! TTTTAGTGTAAAGGTTGAGG+AGG - chr4_4:60472104-60472123 Msa0692120:CDS 35.0%
TTTGTTCATTCACCAGCTAT+TGG - chr4_4:60472216-60472235 Msa0692120:five_prime_UTR 35.0%
ATGCAAAAAAGACCAATAGC+TGG + chr4_4:60472231-60472250 None:intergenic 35.0%
GTGACTTTGGTTGTGTTTGT+GGG + chr4_4:60470519-60470538 None:intergenic 40.0%
CAAAGTCACCAACCAAAGAA+GGG - chr4_4:60470530-60470549 Msa0692120:three_prime_UTR 40.0%
CTATGTTACCCTTCTTTGGT+TGG + chr4_4:60470541-60470560 None:intergenic 40.0%
! GGAAGGGTTTAAGGTTAAGT+TGG + chr4_4:60470669-60470688 None:intergenic 40.0%
AAGGAACATGGAAGGGTTTA+AGG + chr4_4:60470678-60470697 None:intergenic 40.0%
GCCATAAAGAGAAAGGAACA+TGG + chr4_4:60470690-60470709 None:intergenic 40.0%
AGAAGAGGAGAGAGAAAGTT+GGG + chr4_4:60470766-60470785 None:intergenic 40.0%
AAGAAGAGGAGAGAGAAAGT+TGG + chr4_4:60470767-60470786 None:intergenic 40.0%
AAGTTCATGGATGGAAGAAG+AGG + chr4_4:60470781-60470800 None:intergenic 40.0%
TGAACGAAGAAGTTCATGGA+TGG + chr4_4:60470790-60470809 None:intergenic 40.0%
GTGTAGGACTTTATCTCTTC+TGG + chr4_4:60470837-60470856 None:intergenic 40.0%
ATCACTGCTAATCTCACCTA+TGG - chr4_4:60470942-60470961 Msa0692120:CDS 40.0%
AACACCAATGAGACTTCCAT+AGG + chr4_4:60470961-60470980 None:intergenic 40.0%
GCATGGAGTATAGTTCCAAA+AGG + chr4_4:60471221-60471240 None:intergenic 40.0%
! TAAATTAGCCCCACCCATTT+TGG + chr4_4:60471748-60471767 None:intergenic 40.0%
! GTGAGTTGAAGAATCATGTG+AGG - chr4_4:60471941-60471960 Msa0692120:CDS 40.0%
GTTGAAGAATCATGTGAGGA+AGG - chr4_4:60471945-60471964 Msa0692120:CDS 40.0%
! AAGATGCCAACTCAGTTTTC+AGG - chr4_4:60471997-60472016 Msa0692120:CDS 40.0%
TTGAAGCCTGAAAACTGAGT+TGG + chr4_4:60472006-60472025 None:intergenic 40.0%
AGAACATGTAAAGATGTGGG+TGG - chr4_4:60472137-60472156 Msa0692120:CDS 40.0%
GAACATGTAAAGATGTGGGT+GGG - chr4_4:60472138-60472157 Msa0692120:CDS 40.0%
AACATGTAAAGATGTGGGTG+GGG - chr4_4:60472139-60472158 Msa0692120:CDS 40.0%
GTGACTTTGGTTGTGTTTGT+GGG + chr4_4:60470519-60470538 None:intergenic 40.0%
CAAAGTCACCAACCAAAGAA+GGG - chr4_4:60470530-60470549 Msa0692120:three_prime_UTR 40.0%
CTATGTTACCCTTCTTTGGT+TGG + chr4_4:60470541-60470560 None:intergenic 40.0%
! GGAAGGGTTTAAGGTTAAGT+TGG + chr4_4:60470669-60470688 None:intergenic 40.0%
AAGGAACATGGAAGGGTTTA+AGG + chr4_4:60470678-60470697 None:intergenic 40.0%
GCCATAAAGAGAAAGGAACA+TGG + chr4_4:60470690-60470709 None:intergenic 40.0%
AGAAGAGGAGAGAGAAAGTT+GGG + chr4_4:60470766-60470785 None:intergenic 40.0%
AAGAAGAGGAGAGAGAAAGT+TGG + chr4_4:60470767-60470786 None:intergenic 40.0%
AAGTTCATGGATGGAAGAAG+AGG + chr4_4:60470781-60470800 None:intergenic 40.0%
TGAACGAAGAAGTTCATGGA+TGG + chr4_4:60470790-60470809 None:intergenic 40.0%
GTGTAGGACTTTATCTCTTC+TGG + chr4_4:60470837-60470856 None:intergenic 40.0%
ATCACTGCTAATCTCACCTA+TGG - chr4_4:60470942-60470961 Msa0692120:CDS 40.0%
AACACCAATGAGACTTCCAT+AGG + chr4_4:60470961-60470980 None:intergenic 40.0%
GCATGGAGTATAGTTCCAAA+AGG + chr4_4:60471221-60471240 None:intergenic 40.0%
! TAAATTAGCCCCACCCATTT+TGG + chr4_4:60471748-60471767 None:intergenic 40.0%
! GTGAGTTGAAGAATCATGTG+AGG - chr4_4:60471941-60471960 Msa0692120:CDS 40.0%
GTTGAAGAATCATGTGAGGA+AGG - chr4_4:60471945-60471964 Msa0692120:CDS 40.0%
! AAGATGCCAACTCAGTTTTC+AGG - chr4_4:60471997-60472016 Msa0692120:CDS 40.0%
TTGAAGCCTGAAAACTGAGT+TGG + chr4_4:60472006-60472025 None:intergenic 40.0%
AGAACATGTAAAGATGTGGG+TGG - chr4_4:60472137-60472156 Msa0692120:CDS 40.0%
GAACATGTAAAGATGTGGGT+GGG - chr4_4:60472138-60472157 Msa0692120:CDS 40.0%
AACATGTAAAGATGTGGGTG+GGG - chr4_4:60472139-60472158 Msa0692120:CDS 40.0%
GGTGACTTTGGTTGTGTTTG+TGG + chr4_4:60470520-60470539 None:intergenic 45.0%
!! CCTTCTTTGGTTGGTGACTT+TGG + chr4_4:60470532-60470551 None:intergenic 45.0%
CCAAAGTCACCAACCAAAGA+AGG - chr4_4:60470529-60470548 Msa0692120:three_prime_UTR 45.0%
GAAGAGGAGAGAGAAAGTTG+GGG + chr4_4:60470765-60470784 None:intergenic 45.0%
CTCACCTATGGAAGTCTCAT+TGG - chr4_4:60470954-60470973 Msa0692120:CDS 45.0%
!! GGAAGTCTCATTGGTGTTGA+AGG - chr4_4:60470963-60470982 Msa0692120:intron 45.0%
CCTTGAGGTTTACAATGAGG+TGG + chr4_4:60471119-60471138 None:intergenic 45.0%
CCACCTCATTGTAAACCTCA+AGG - chr4_4:60471116-60471135 Msa0692120:intron 45.0%
! AGATCTGCTACTACCCCTTT+TGG - chr4_4:60471203-60471222 Msa0692120:intron 45.0%
! GCTAATTGGTTGAGTTGTGC+AGG - chr4_4:60471919-60471938 Msa0692120:CDS 45.0%
AATCATGTGAGGAAGGAGAG+AGG - chr4_4:60471952-60471971 Msa0692120:CDS 45.0%
GGTGACTTTGGTTGTGTTTG+TGG + chr4_4:60470520-60470539 None:intergenic 45.0%
!! CCTTCTTTGGTTGGTGACTT+TGG + chr4_4:60470532-60470551 None:intergenic 45.0%
CCAAAGTCACCAACCAAAGA+AGG - chr4_4:60470529-60470548 Msa0692120:three_prime_UTR 45.0%
GAAGAGGAGAGAGAAAGTTG+GGG + chr4_4:60470765-60470784 None:intergenic 45.0%
CTCACCTATGGAAGTCTCAT+TGG - chr4_4:60470954-60470973 Msa0692120:CDS 45.0%
!! GGAAGTCTCATTGGTGTTGA+AGG - chr4_4:60470963-60470982 Msa0692120:intron 45.0%
CCTTGAGGTTTACAATGAGG+TGG + chr4_4:60471119-60471138 None:intergenic 45.0%
CCACCTCATTGTAAACCTCA+AGG - chr4_4:60471116-60471135 Msa0692120:intron 45.0%
! AGATCTGCTACTACCCCTTT+TGG - chr4_4:60471203-60471222 Msa0692120:intron 45.0%
! GCTAATTGGTTGAGTTGTGC+AGG - chr4_4:60471919-60471938 Msa0692120:CDS 45.0%
AATCATGTGAGGAAGGAGAG+AGG - chr4_4:60471952-60471971 Msa0692120:CDS 45.0%
AAAGTTGGGGAGAGAGGAGA+AGG + chr4_4:60470752-60470771 None:intergenic 50.0%
AGAGAGAAAGTTGGGGAGAG+AGG + chr4_4:60470758-60470777 None:intergenic 50.0%
CGTTCAAAAGGCTTACCAGC+TGG - chr4_4:60470804-60470823 Msa0692120:three_prime_UTR 50.0%
TCTTCTGGTAAGAGACCAGC+TGG + chr4_4:60470822-60470841 None:intergenic 50.0%
GAGGAAGGAGAGAGGATTTG+AGG - chr4_4:60471960-60471979 Msa0692120:CDS 50.0%
! AGAGAGGATTTGAGGCAGTG+AGG - chr4_4:60471968-60471987 Msa0692120:CDS 50.0%
AAAGTTGGGGAGAGAGGAGA+AGG + chr4_4:60470752-60470771 None:intergenic 50.0%
AGAGAGAAAGTTGGGGAGAG+AGG + chr4_4:60470758-60470777 None:intergenic 50.0%
CGTTCAAAAGGCTTACCAGC+TGG - chr4_4:60470804-60470823 Msa0692120:three_prime_UTR 50.0%
TCTTCTGGTAAGAGACCAGC+TGG + chr4_4:60470822-60470841 None:intergenic 50.0%
GAGGAAGGAGAGAGGATTTG+AGG - chr4_4:60471960-60471979 Msa0692120:CDS 50.0%
! AGAGAGGATTTGAGGCAGTG+AGG - chr4_4:60471968-60471987 Msa0692120:CDS 50.0%
AGAGGAGAAGGAGGAGAGAG+AGG + chr4_4:60470740-60470759 None:intergenic 55.0%
AGAGGAGAAGGAGGAGAGAG+AGG + chr4_4:60470740-60470759 None:intergenic 55.0%
GTTGGGGAGAGAGGAGAAGG+AGG + chr4_4:60470749-60470768 None:intergenic 60.0%
GTTGGGGAGAGAGGAGAAGG+AGG + chr4_4:60470749-60470768 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 60470508 60472411 60470508 ID=Msa0692120;Name=Msa0692120
chr4_4 mRNA 60470508 60472411 60470508 ID=Msa0692120-mRNA-1;Parent=Msa0692120;Name=Msa0692120-mRNA-1;_AED=0.01;_eAED=0.01;_QI=197|1|1|1|1|1|2|418|161
chr4_4 exon 60470508 60470978 60470508 ID=Msa0692120-mRNA-1:exon:16591;Parent=Msa0692120-mRNA-1
chr4_4 exon 60471782 60472411 60471782 ID=Msa0692120-mRNA-1:exon:16590;Parent=Msa0692120-mRNA-1
chr4_4 five_prime_UTR 60472215 60472411 60472215 ID=Msa0692120-mRNA-1:five_prime_utr;Parent=Msa0692120-mRNA-1
chr4_4 CDS 60471782 60472214 60471782 ID=Msa0692120-mRNA-1:cds;Parent=Msa0692120-mRNA-1
chr4_4 CDS 60470926 60470978 60470926 ID=Msa0692120-mRNA-1:cds;Parent=Msa0692120-mRNA-1
chr4_4 three_prime_UTR 60470508 60470925 60470508 ID=Msa0692120-mRNA-1:three_prime_utr;Parent=Msa0692120-mRNA-1
Gene Sequence

>Msa0692120

ATGGCATTCTATAACACTCTTCTCTCTTTCTTCCTCTCTCTCCTCCTTCTCCTCTCTCCCCAACTTTCTCTCTCCTCTTCTTCCATCCATGAACTTCTTCGTTCAAAAGGCTTACCAGCTGGTCTCTTACCAGAAGAGATAAAGTCCTACACTTTCTCAGAAAATGGTCACTTAGAAGTGTTCCTTGAATCACCTTGTTTAACAAAGTATGAAAACAGAGTCTACTTTGAACAAGTTATCACTGCTAATCTCACCTATGGAAGTCTCATTGGTGTTGAAGGTTTGCAACAAGAAGAGCTTTTTGTTTGGTTACCTGTTAAAGATATCATTGTTGATGATCCATCTTCTGGTTTGATTCTCTTTGACATTGGTCTTGCTTATAAACAACTCTCTTTCTCTCTCTTTGAAGTTCCACCTCATTGTAAACCTCAAGGTGAGTTGAAGAATCATGTGAGGAAGGAGAGAGGATTTGAGGCAGTGAGGTAG
Protein sequence

>Msa0692120

MAFYNTLLSFFLSLLLLLSPQLSLSSSSIHELLRSKGLPAGLLPEEIKSYTFSENGHLEVFLESPCLTKYENRVYFEQVITANLTYGSLIGVEGLQQEELFVWLPVKDIIVDDPSSGLILFDIGLAYKQLSFSLFEVPPHCKPQGELKNHVRKERGFEAVR*