AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0556980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0556980 MtrunA17_Chr4g0045961 96.350 137 4 1 1 136 1 137 1.40e-94 270
Msa0556980 MtrunA17_Chr4g0045971 86.765 136 17 1 3 138 53 187 1.24e-82 240
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0556980 AT1G29040.1 87.395 119 15 0 20 138 80 198 8.08e-77 226
Msa0556980 AT1G29040.5 80.672 119 14 1 20 138 80 189 1.36e-66 200
Msa0556980 AT1G29040.2 76.471 119 13 1 20 138 80 183 5.21e-62 188
Msa0556980 AT1G29040.4 84.286 70 11 0 20 89 80 149 1.03e-39 131
Msa0556980 AT1G29040.3 85.366 41 6 0 20 60 80 120 1.14e-20 82.8

Find 53 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATTCCTGGTGTCAGTTT+TGG 0.269189 4_1:-55228707 Msa0556980:CDS
TTTGGTTTAGGTTCCATTCC+TGG 0.328470 4_1:-55228719 Msa0556980:intron
AATGAATCATCTCTTATTTG+TGG 0.365797 4_1:-55229250 Msa0556980:CDS
AAAGTGTCTCTGGAGAAAAT+TGG 0.385180 4_1:+55229405 None:intergenic
CTGCAGCAATGAGAGCTTGT+AGG 0.394064 4_1:-55229185 Msa0556980:CDS
TATATGCCATAGCTCTTGTT+TGG 0.395661 4_1:-55228152 Msa0556980:three_prime_UTR
CCATGGCTGCGGTTGTAGCT+CGG 0.399517 4_1:+55229316 None:intergenic
CATGGCTGCGGTTGTAGCTC+GGG 0.411428 4_1:+55229317 None:intergenic
TCTCTGGAGAAAATTGGAAT+TGG 0.416120 4_1:+55229411 None:intergenic
TCACTCTCTCCAACAATCTC+TGG 0.424802 4_1:-55228651 Msa0556980:CDS
ATAGTGTTGCACTATTAGTA+TGG 0.426674 4_1:-55228099 Msa0556980:three_prime_UTR
TCTCAATATCCAGAGATTGT+TGG 0.427681 4_1:+55228642 None:intergenic
GGGGATTGAATTAGAAGAAA+TGG 0.433548 4_1:+55229158 None:intergenic
AATATCAGAAGTACATCAAC+AGG 0.443530 4_1:+55228222 None:intergenic
TTCGCTTTGTTCAACTTCCA+TGG 0.445748 4_1:+55229299 None:intergenic
GAGAAAGATAAAGAGGTTTG+TGG 0.447327 4_1:+55229375 None:intergenic
ATTATTTGAGAAAGATAAAG+AGG 0.456216 4_1:+55229368 None:intergenic
TGGTAAGAGAAAAGTGTCTC+TGG 0.459123 4_1:+55229395 None:intergenic
ATGAAATTGAAGATCAAGCT+AGG 0.461847 4_1:-55228680 Msa0556980:CDS
TCATCTCTTATTTGTGGAGA+TGG 0.467098 4_1:-55229244 Msa0556980:CDS
TTATTACCTCTGCGAAAAGC+GGG 0.473127 4_1:+55229138 None:intergenic
ATACTAATAGTGCAACACTA+TGG 0.480492 4_1:+55228101 None:intergenic
TGGGGTGCTTGTGGAAGAGA+TGG 0.491243 4_1:-55228333 Msa0556980:CDS
TGCAGCAATGAGAGCTTGTA+GGG 0.494719 4_1:-55229184 Msa0556980:CDS
GGTTGTAGCTCGGGATTGAA+AGG 0.495111 4_1:+55229326 None:intergenic
CATTGTTTGTGAAGATGAAT+CGG 0.496838 4_1:+55229269 None:intergenic
CATCTCTTATTTGTGGAGAT+GGG 0.499868 4_1:-55229243 Msa0556980:CDS
TTGTTCAACTTCCATGGCTG+CGG 0.516027 4_1:+55229305 None:intergenic
AATGTTGAAGTTGTGGATGG+TGG 0.522422 4_1:-55228371 Msa0556980:CDS
CCGAGCTACAACCGCAGCCA+TGG 0.528834 4_1:-55229316 Msa0556980:exon
ATGCTCAAGTGGGGTGCTTG+TGG 0.529287 4_1:-55228342 Msa0556980:CDS
TTATTTGTGGAGATGGGTGT+CGG 0.531510 4_1:-55229237 Msa0556980:CDS
GTTGTAGCTCGGGATTGAAA+GGG 0.538825 4_1:+55229327 None:intergenic
GGTGGTTTGATATGCTCAAG+TGG 0.543111 4_1:-55228353 Msa0556980:CDS
TGAAATTGAAGATCAAGCTA+GGG 0.544152 4_1:-55228679 Msa0556980:CDS
AGAGATGGGTGACAAGAATG+AGG 0.552128 4_1:-55228318 Msa0556980:CDS
ACCAAAACTGACACCAGGAA+TGG 0.557527 4_1:+55228706 None:intergenic
CGATGACCAAACAAGAGCTA+TGG 0.568381 4_1:+55228146 None:intergenic
GTGGTTTGATATGCTCAAGT+GGG 0.568560 4_1:-55228352 Msa0556980:CDS
GTGAATGCTGCTGTTTATGT+CGG 0.572315 4_1:-55228284 Msa0556980:CDS
AGAGATTGTTGGAGAGAGTG+AGG 0.584917 4_1:+55228653 None:intergenic
GGGGTGCTTGTGGAAGAGAT+GGG 0.587583 4_1:-55228332 Msa0556980:CDS
ATGGAAGTTGAACAAAGCGA+AGG 0.594191 4_1:-55229297 Msa0556980:CDS
ATTTGACCAAAACTGACACC+AGG 0.602977 4_1:+55228701 None:intergenic
TTGAATGTTGAAGTTGTGGA+TGG 0.604010 4_1:-55228374 Msa0556980:CDS
ATTATTACCTCTGCGAAAAG+CGG 0.612273 4_1:+55229137 None:intergenic
ATAGTGCAACACTATGGCAA+TGG 0.632402 4_1:+55228107 None:intergenic
TGGAAGTTGAACAAAGCGAA+GGG 0.636048 4_1:-55229296 Msa0556980:CDS
TATTACCTCTGCGAAAAGCG+GGG 0.652437 4_1:+55229139 None:intergenic
GAAATTGAAGATCAAGCTAG+GGG 0.679857 4_1:-55228678 Msa0556980:CDS
GTGTCGGTTACGATCAACAC+GGG 0.696727 4_1:-55229221 Msa0556980:CDS
TGGTTTGATATGCTCAAGTG+GGG 0.708142 4_1:-55228351 Msa0556980:CDS
GGTGTCGGTTACGATCAACA+CGG 0.778549 4_1:-55229222 Msa0556980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATAATCTTTTTCTTTTTT+TGG - chr4_1:55228336-55228355 Msa0556980:CDS 10.0%
!! TTGATTTAATAGTTAATAAT+AGG + chr4_1:55228365-55228384 None:intergenic 10.0%
!! ATTATTAACTATTAAATCAA+TGG - chr4_1:55228365-55228384 Msa0556980:CDS 10.0%
!! TATGTTTATAAATTATAACT+AGG - chr4_1:55228420-55228439 Msa0556980:intron 10.0%
!! ATGTTTATAAATTATAACTA+GGG - chr4_1:55228421-55228440 Msa0556980:intron 10.0%
!!! TAATAATCTTTTTCTTTTTT+TGG - chr4_1:55228336-55228355 Msa0556980:CDS 10.0%
!! TTGATTTAATAGTTAATAAT+AGG + chr4_1:55228365-55228384 None:intergenic 10.0%
!! ATTATTAACTATTAAATCAA+TGG - chr4_1:55228365-55228384 Msa0556980:CDS 10.0%
!! TATGTTTATAAATTATAACT+AGG - chr4_1:55228420-55228439 Msa0556980:intron 10.0%
!! ATGTTTATAAATTATAACTA+GGG - chr4_1:55228421-55228440 Msa0556980:intron 10.0%
!! ATAAACATAACAATTATCAT+TGG + chr4_1:55228409-55228428 None:intergenic 15.0%
!! AAAAAAACCATTGATAAAAT+AGG + chr4_1:55228944-55228963 None:intergenic 15.0%
!! TTATAAATAATAGATAGATG+TGG + chr4_1:55229006-55229025 None:intergenic 15.0%
!! ATAAACATAACAATTATCAT+TGG + chr4_1:55228409-55228428 None:intergenic 15.0%
!! AAAAAAACCATTGATAAAAT+AGG + chr4_1:55228944-55228963 None:intergenic 15.0%
!! TTATAAATAATAGATAGATG+TGG + chr4_1:55229006-55229025 None:intergenic 15.0%
!! ATTATTTGAGAAAGATAAAG+AGG + chr4_1:55228092-55228111 None:intergenic 20.0%
!!! TAATTGATAATATCTAGTTC+AGG - chr4_1:55228552-55228571 Msa0556980:intron 20.0%
!! AATTTCTAATGAGATGAATT+TGG - chr4_1:55228720-55228739 Msa0556980:intron 20.0%
!!! ATGAAATCCTATTTTATCAA+TGG - chr4_1:55228934-55228953 Msa0556980:intron 20.0%
!! ATTATTTGAGAAAGATAAAG+AGG + chr4_1:55228092-55228111 None:intergenic 20.0%
!!! TAATTGATAATATCTAGTTC+AGG - chr4_1:55228552-55228571 Msa0556980:intron 20.0%
!! AATTTCTAATGAGATGAATT+TGG - chr4_1:55228720-55228739 Msa0556980:intron 20.0%
!!! ATGAAATCCTATTTTATCAA+TGG - chr4_1:55228934-55228953 Msa0556980:intron 20.0%
!! AATGAATCATCTCTTATTTG+TGG - chr4_1:55228207-55228226 Msa0556980:three_prime_UTR 25.0%
! TTACTTCTTAGTAAGTTCAT+TGG - chr4_1:55228451-55228470 Msa0556980:intron 25.0%
! TCAATTACTCAATAGAGAAA+TGG + chr4_1:55228539-55228558 None:intergenic 25.0%
!! TAATGAGATGAATTTGGTTT+AGG - chr4_1:55228726-55228745 Msa0556980:intron 25.0%
! CAACTTATGGAAATGAAAAA+AGG + chr4_1:55228892-55228911 None:intergenic 25.0%
!! AATTTTGAATGTTGAAGTTG+TGG - chr4_1:55229079-55229098 Msa0556980:intron 25.0%
!! AATGAATCATCTCTTATTTG+TGG - chr4_1:55228207-55228226 Msa0556980:three_prime_UTR 25.0%
! TTACTTCTTAGTAAGTTCAT+TGG - chr4_1:55228451-55228470 Msa0556980:intron 25.0%
! TCAATTACTCAATAGAGAAA+TGG + chr4_1:55228539-55228558 None:intergenic 25.0%
!! TAATGAGATGAATTTGGTTT+AGG - chr4_1:55228726-55228745 Msa0556980:intron 25.0%
! CAACTTATGGAAATGAAAAA+AGG + chr4_1:55228892-55228911 None:intergenic 25.0%
!! AATTTTGAATGTTGAAGTTG+TGG - chr4_1:55229079-55229098 Msa0556980:intron 25.0%
CATTGTTTGTGAAGATGAAT+CGG + chr4_1:55228191-55228210 None:intergenic 30.0%
! TCTTAGTAAGTTCATTGGTT+TGG - chr4_1:55228456-55228475 Msa0556980:intron 30.0%
TACTGTTTAATCAGAATCCA+AGG + chr4_1:55228505-55228524 None:intergenic 30.0%
GAAAATATCTTATGCTCAGA+TGG - chr4_1:55228574-55228593 Msa0556980:intron 30.0%
ATGAAATTGAAGATCAAGCT+AGG - chr4_1:55228777-55228796 Msa0556980:intron 30.0%
TGAAATTGAAGATCAAGCTA+GGG - chr4_1:55228778-55228797 Msa0556980:intron 30.0%
CACATAGAAGAAACAACTTA+TGG + chr4_1:55228905-55228924 None:intergenic 30.0%
!! CATAAGTTGTTTCTTCTATG+TGG - chr4_1:55228903-55228922 Msa0556980:intron 30.0%
GTTATGAGCTTAACTTTGAA+AGG + chr4_1:55228970-55228989 None:intergenic 30.0%
TAATAGATAGATGTGGAAGA+TGG + chr4_1:55228999-55229018 None:intergenic 30.0%
! AATATCAGAAGTACATCAAC+AGG + chr4_1:55229238-55229257 None:intergenic 30.0%
ATACTAATAGTGCAACACTA+TGG + chr4_1:55229359-55229378 None:intergenic 30.0%
ATAGTGTTGCACTATTAGTA+TGG - chr4_1:55229358-55229377 Msa0556980:five_prime_UTR 30.0%
CATTGTTTGTGAAGATGAAT+CGG + chr4_1:55228191-55228210 None:intergenic 30.0%
! TCTTAGTAAGTTCATTGGTT+TGG - chr4_1:55228456-55228475 Msa0556980:intron 30.0%
TACTGTTTAATCAGAATCCA+AGG + chr4_1:55228505-55228524 None:intergenic 30.0%
GAAAATATCTTATGCTCAGA+TGG - chr4_1:55228574-55228593 Msa0556980:intron 30.0%
ATGAAATTGAAGATCAAGCT+AGG - chr4_1:55228777-55228796 Msa0556980:intron 30.0%
TGAAATTGAAGATCAAGCTA+GGG - chr4_1:55228778-55228797 Msa0556980:intron 30.0%
CACATAGAAGAAACAACTTA+TGG + chr4_1:55228905-55228924 None:intergenic 30.0%
!! CATAAGTTGTTTCTTCTATG+TGG - chr4_1:55228903-55228922 Msa0556980:intron 30.0%
GTTATGAGCTTAACTTTGAA+AGG + chr4_1:55228970-55228989 None:intergenic 30.0%
TAATAGATAGATGTGGAAGA+TGG + chr4_1:55228999-55229018 None:intergenic 30.0%
! AATATCAGAAGTACATCAAC+AGG + chr4_1:55229238-55229257 None:intergenic 30.0%
ATACTAATAGTGCAACACTA+TGG + chr4_1:55229359-55229378 None:intergenic 30.0%
ATAGTGTTGCACTATTAGTA+TGG - chr4_1:55229358-55229377 Msa0556980:five_prime_UTR 30.0%
! TCTCTGGAGAAAATTGGAAT+TGG + chr4_1:55228049-55228068 None:intergenic 35.0%
! AAAGTGTCTCTGGAGAAAAT+TGG + chr4_1:55228055-55228074 None:intergenic 35.0%
GAGAAAGATAAAGAGGTTTG+TGG + chr4_1:55228085-55228104 None:intergenic 35.0%
! TCATCTCTTATTTGTGGAGA+TGG - chr4_1:55228213-55228232 Msa0556980:three_prime_UTR 35.0%
! CATCTCTTATTTGTGGAGAT+GGG - chr4_1:55228214-55228233 Msa0556980:three_prime_UTR 35.0%
GGGGATTGAATTAGAAGAAA+TGG + chr4_1:55228302-55228321 None:intergenic 35.0%
ATTATTACCTCTGCGAAAAG+CGG + chr4_1:55228323-55228342 None:intergenic 35.0%
!! CATTGGTTTGGTTTGTTTCT+TGG - chr4_1:55228468-55228487 Msa0556980:intron 35.0%
GAAATTGAAGATCAAGCTAG+GGG - chr4_1:55228779-55228798 Msa0556980:intron 35.0%
TCTCAATATCCAGAGATTGT+TGG + chr4_1:55228818-55228837 None:intergenic 35.0%
GGGTGAAAGAAACTTCAATA+AGG + chr4_1:55229032-55229051 None:intergenic 35.0%
TTGAATGTTGAAGTTGTGGA+TGG - chr4_1:55229083-55229102 Msa0556980:intron 35.0%
GAAAAACTGAAATCTGCACA+AGG + chr4_1:55229212-55229231 None:intergenic 35.0%
TATATGCCATAGCTCTTGTT+TGG - chr4_1:55229305-55229324 Msa0556980:exon 35.0%
! TCTCTGGAGAAAATTGGAAT+TGG + chr4_1:55228049-55228068 None:intergenic 35.0%
! AAAGTGTCTCTGGAGAAAAT+TGG + chr4_1:55228055-55228074 None:intergenic 35.0%
GAGAAAGATAAAGAGGTTTG+TGG + chr4_1:55228085-55228104 None:intergenic 35.0%
! TCATCTCTTATTTGTGGAGA+TGG - chr4_1:55228213-55228232 Msa0556980:three_prime_UTR 35.0%
! CATCTCTTATTTGTGGAGAT+GGG - chr4_1:55228214-55228233 Msa0556980:three_prime_UTR 35.0%
GGGGATTGAATTAGAAGAAA+TGG + chr4_1:55228302-55228321 None:intergenic 35.0%
ATTATTACCTCTGCGAAAAG+CGG + chr4_1:55228323-55228342 None:intergenic 35.0%
!! CATTGGTTTGGTTTGTTTCT+TGG - chr4_1:55228468-55228487 Msa0556980:intron 35.0%
GAAATTGAAGATCAAGCTAG+GGG - chr4_1:55228779-55228798 Msa0556980:intron 35.0%
TCTCAATATCCAGAGATTGT+TGG + chr4_1:55228818-55228837 None:intergenic 35.0%
GGGTGAAAGAAACTTCAATA+AGG + chr4_1:55229032-55229051 None:intergenic 35.0%
TTGAATGTTGAAGTTGTGGA+TGG - chr4_1:55229083-55229102 Msa0556980:intron 35.0%
GAAAAACTGAAATCTGCACA+AGG + chr4_1:55229212-55229231 None:intergenic 35.0%
TATATGCCATAGCTCTTGTT+TGG - chr4_1:55229305-55229324 Msa0556980:exon 35.0%
!! TGGTAAGAGAAAAGTGTCTC+TGG + chr4_1:55228065-55228084 None:intergenic 40.0%
! TTCGCTTTGTTCAACTTCCA+TGG + chr4_1:55228161-55228180 None:intergenic 40.0%
ATGGAAGTTGAACAAAGCGA+AGG - chr4_1:55228160-55228179 Msa0556980:three_prime_UTR 40.0%
TGGAAGTTGAACAAAGCGAA+GGG - chr4_1:55228161-55228180 Msa0556980:three_prime_UTR 40.0%
TTATTTGTGGAGATGGGTGT+CGG - chr4_1:55228220-55228239 Msa0556980:three_prime_UTR 40.0%
TTATTACCTCTGCGAAAAGC+GGG + chr4_1:55228322-55228341 None:intergenic 40.0%
! GACTGAAATTGCCACTGATT+TGG - chr4_1:55228604-55228623 Msa0556980:intron 40.0%
CCACTTAGATTCCAAATCAG+TGG + chr4_1:55228618-55228637 None:intergenic 40.0%
!! CCACTGATTTGGAATCTAAG+TGG - chr4_1:55228615-55228634 Msa0556980:intron 40.0%
! TTTGGTTTAGGTTCCATTCC+TGG - chr4_1:55228738-55228757 Msa0556980:intron 40.0%
ATTTGACCAAAACTGACACC+AGG + chr4_1:55228759-55228778 None:intergenic 40.0%
! TGCAATTGCAATGTTGGTGA+GGG + chr4_1:55229052-55229071 None:intergenic 40.0%
AATGTTGAAGTTGTGGATGG+TGG - chr4_1:55229086-55229105 Msa0556980:intron 40.0%
GTGGTTTGATATGCTCAAGT+GGG - chr4_1:55229105-55229124 Msa0556980:intron 40.0%
TGGTTTGATATGCTCAAGTG+GGG - chr4_1:55229106-55229125 Msa0556980:intron 40.0%
! GTGAATGCTGCTGTTTATGT+CGG - chr4_1:55229173-55229192 Msa0556980:CDS 40.0%
ATAGTGCAACACTATGGCAA+TGG + chr4_1:55229353-55229372 None:intergenic 40.0%
! GTGACGAATGATTTTGTTGG+AGG + chr4_1:55229436-55229455 None:intergenic 40.0%
!! TGGTAAGAGAAAAGTGTCTC+TGG + chr4_1:55228065-55228084 None:intergenic 40.0%
! TTCGCTTTGTTCAACTTCCA+TGG + chr4_1:55228161-55228180 None:intergenic 40.0%
ATGGAAGTTGAACAAAGCGA+AGG - chr4_1:55228160-55228179 Msa0556980:three_prime_UTR 40.0%
TGGAAGTTGAACAAAGCGAA+GGG - chr4_1:55228161-55228180 Msa0556980:three_prime_UTR 40.0%
TTATTTGTGGAGATGGGTGT+CGG - chr4_1:55228220-55228239 Msa0556980:three_prime_UTR 40.0%
TTATTACCTCTGCGAAAAGC+GGG + chr4_1:55228322-55228341 None:intergenic 40.0%
! GACTGAAATTGCCACTGATT+TGG - chr4_1:55228604-55228623 Msa0556980:intron 40.0%
CCACTTAGATTCCAAATCAG+TGG + chr4_1:55228618-55228637 None:intergenic 40.0%
!! CCACTGATTTGGAATCTAAG+TGG - chr4_1:55228615-55228634 Msa0556980:intron 40.0%
! TTTGGTTTAGGTTCCATTCC+TGG - chr4_1:55228738-55228757 Msa0556980:intron 40.0%
ATTTGACCAAAACTGACACC+AGG + chr4_1:55228759-55228778 None:intergenic 40.0%
! TGCAATTGCAATGTTGGTGA+GGG + chr4_1:55229052-55229071 None:intergenic 40.0%
AATGTTGAAGTTGTGGATGG+TGG - chr4_1:55229086-55229105 Msa0556980:intron 40.0%
GTGGTTTGATATGCTCAAGT+GGG - chr4_1:55229105-55229124 Msa0556980:intron 40.0%
TGGTTTGATATGCTCAAGTG+GGG - chr4_1:55229106-55229125 Msa0556980:intron 40.0%
! GTGAATGCTGCTGTTTATGT+CGG - chr4_1:55229173-55229192 Msa0556980:CDS 40.0%
ATAGTGCAACACTATGGCAA+TGG + chr4_1:55229353-55229372 None:intergenic 40.0%
! GTGACGAATGATTTTGTTGG+AGG + chr4_1:55229436-55229455 None:intergenic 40.0%
GTTGTAGCTCGGGATTGAAA+GGG + chr4_1:55228133-55228152 None:intergenic 45.0%
TTGTTCAACTTCCATGGCTG+CGG + chr4_1:55228155-55228174 None:intergenic 45.0%
TGCAGCAATGAGAGCTTGTA+GGG - chr4_1:55228273-55228292 Msa0556980:exon 45.0%
TATTACCTCTGCGAAAAGCG+GGG + chr4_1:55228321-55228340 None:intergenic 45.0%
! TCTTGGTGTGACTCATACCT+TGG - chr4_1:55228485-55228504 Msa0556980:intron 45.0%
!! TCCATTCCTGGTGTCAGTTT+TGG - chr4_1:55228750-55228769 Msa0556980:intron 45.0%
ACCAAAACTGACACCAGGAA+TGG + chr4_1:55228754-55228773 None:intergenic 45.0%
AGAGATTGTTGGAGAGAGTG+AGG + chr4_1:55228807-55228826 None:intergenic 45.0%
TCACTCTCTCCAACAATCTC+TGG - chr4_1:55228806-55228825 Msa0556980:intron 45.0%
ACTGATGCAGGTACTCACTA+GGG + chr4_1:55228858-55228877 None:intergenic 45.0%
CTGCAATTGCAATGTTGGTG+AGG + chr4_1:55229053-55229072 None:intergenic 45.0%
CACCAACATTGCAATTGCAG+CGG - chr4_1:55229053-55229072 Msa0556980:intron 45.0%
ACCAACATTGCAATTGCAGC+GGG - chr4_1:55229054-55229073 Msa0556980:intron 45.0%
GGTGGTTTGATATGCTCAAG+TGG - chr4_1:55229104-55229123 Msa0556980:intron 45.0%
AGAGATGGGTGACAAGAATG+AGG - chr4_1:55229139-55229158 Msa0556980:intron 45.0%
CGATGACCAAACAAGAGCTA+TGG + chr4_1:55229314-55229333 None:intergenic 45.0%
GTTGTAGCTCGGGATTGAAA+GGG + chr4_1:55228133-55228152 None:intergenic 45.0%
TTGTTCAACTTCCATGGCTG+CGG + chr4_1:55228155-55228174 None:intergenic 45.0%
TGCAGCAATGAGAGCTTGTA+GGG - chr4_1:55228273-55228292 Msa0556980:exon 45.0%
TATTACCTCTGCGAAAAGCG+GGG + chr4_1:55228321-55228340 None:intergenic 45.0%
! TCTTGGTGTGACTCATACCT+TGG - chr4_1:55228485-55228504 Msa0556980:intron 45.0%
!! TCCATTCCTGGTGTCAGTTT+TGG - chr4_1:55228750-55228769 Msa0556980:intron 45.0%
ACCAAAACTGACACCAGGAA+TGG + chr4_1:55228754-55228773 None:intergenic 45.0%
AGAGATTGTTGGAGAGAGTG+AGG + chr4_1:55228807-55228826 None:intergenic 45.0%
TCACTCTCTCCAACAATCTC+TGG - chr4_1:55228806-55228825 Msa0556980:intron 45.0%
ACTGATGCAGGTACTCACTA+GGG + chr4_1:55228858-55228877 None:intergenic 45.0%
CTGCAATTGCAATGTTGGTG+AGG + chr4_1:55229053-55229072 None:intergenic 45.0%
CACCAACATTGCAATTGCAG+CGG - chr4_1:55229053-55229072 Msa0556980:intron 45.0%
ACCAACATTGCAATTGCAGC+GGG - chr4_1:55229054-55229073 Msa0556980:intron 45.0%
GGTGGTTTGATATGCTCAAG+TGG - chr4_1:55229104-55229123 Msa0556980:intron 45.0%
AGAGATGGGTGACAAGAATG+AGG - chr4_1:55229139-55229158 Msa0556980:intron 45.0%
CGATGACCAAACAAGAGCTA+TGG + chr4_1:55229314-55229333 None:intergenic 45.0%
GGTTGTAGCTCGGGATTGAA+AGG + chr4_1:55228134-55228153 None:intergenic 50.0%
!! GGTGTCGGTTACGATCAACA+CGG - chr4_1:55228235-55228254 Msa0556980:three_prime_UTR 50.0%
!! GTGTCGGTTACGATCAACAC+GGG - chr4_1:55228236-55228255 Msa0556980:three_prime_UTR 50.0%
CTGCAGCAATGAGAGCTTGT+AGG - chr4_1:55228272-55228291 Msa0556980:exon 50.0%
CACTGATGCAGGTACTCACT+AGG + chr4_1:55228859-55228878 None:intergenic 50.0%
CTAGTGAGTACCTGCATCAG+TGG - chr4_1:55228857-55228876 Msa0556980:intron 50.0%
GTACCTGCATCAGTGGAAAC+TGG - chr4_1:55228864-55228883 Msa0556980:intron 50.0%
TCCCGCTGCAATTGCAATGT+TGG + chr4_1:55229058-55229077 None:intergenic 50.0%
GGTTGTAGCTCGGGATTGAA+AGG + chr4_1:55228134-55228153 None:intergenic 50.0%
!! GGTGTCGGTTACGATCAACA+CGG - chr4_1:55228235-55228254 Msa0556980:three_prime_UTR 50.0%
!! GTGTCGGTTACGATCAACAC+GGG - chr4_1:55228236-55228255 Msa0556980:three_prime_UTR 50.0%
CTGCAGCAATGAGAGCTTGT+AGG - chr4_1:55228272-55228291 Msa0556980:exon 50.0%
CACTGATGCAGGTACTCACT+AGG + chr4_1:55228859-55228878 None:intergenic 50.0%
CTAGTGAGTACCTGCATCAG+TGG - chr4_1:55228857-55228876 Msa0556980:intron 50.0%
GTACCTGCATCAGTGGAAAC+TGG - chr4_1:55228864-55228883 Msa0556980:intron 50.0%
TCCCGCTGCAATTGCAATGT+TGG + chr4_1:55229058-55229077 None:intergenic 50.0%
!! TCAATCCCCGCTTTTCGCAG+AGG - chr4_1:55228313-55228332 Msa0556980:CDS 55.0%
GCACCAGTTTCCACTGATGC+AGG + chr4_1:55228870-55228889 None:intergenic 55.0%
! ATGCTCAAGTGGGGTGCTTG+TGG - chr4_1:55229115-55229134 Msa0556980:intron 55.0%
! TGGGGTGCTTGTGGAAGAGA+TGG - chr4_1:55229124-55229143 Msa0556980:intron 55.0%
! GGGGTGCTTGTGGAAGAGAT+GGG - chr4_1:55229125-55229144 Msa0556980:intron 55.0%
!! TCAATCCCCGCTTTTCGCAG+AGG - chr4_1:55228313-55228332 Msa0556980:CDS 55.0%
GCACCAGTTTCCACTGATGC+AGG + chr4_1:55228870-55228889 None:intergenic 55.0%
! ATGCTCAAGTGGGGTGCTTG+TGG - chr4_1:55229115-55229134 Msa0556980:intron 55.0%
! TGGGGTGCTTGTGGAAGAGA+TGG - chr4_1:55229124-55229143 Msa0556980:intron 55.0%
! GGGGTGCTTGTGGAAGAGAT+GGG - chr4_1:55229125-55229144 Msa0556980:intron 55.0%
CATGGCTGCGGTTGTAGCTC+GGG + chr4_1:55228143-55228162 None:intergenic 60.0%
CCATGGCTGCGGTTGTAGCT+CGG + chr4_1:55228144-55228163 None:intergenic 60.0%
CATGGCTGCGGTTGTAGCTC+GGG + chr4_1:55228143-55228162 None:intergenic 60.0%
CCATGGCTGCGGTTGTAGCT+CGG + chr4_1:55228144-55228163 None:intergenic 60.0%
CCGAGCTACAACCGCAGCCA+TGG - chr4_1:55228141-55228160 Msa0556980:three_prime_UTR 65.0%
CCGAGCTACAACCGCAGCCA+TGG - chr4_1:55228141-55228160 Msa0556980:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 55228022 55229457 55228022 ID=Msa0556980;Name=Msa0556980
chr4_1 mRNA 55228022 55229457 55228022 ID=Msa0556980-mRNA-1;Parent=Msa0556980;Name=Msa0556980-mRNA-1;_AED=0.28;_eAED=0.28;_QI=138|1|1|1|1|1|3|255|138
chr4_1 exon 55228022 55228406 55228022 ID=Msa0556980-mRNA-1:exon:16482;Parent=Msa0556980-mRNA-1
chr4_1 exon 55228620 55228731 55228620 ID=Msa0556980-mRNA-1:exon:16481;Parent=Msa0556980-mRNA-1
chr4_1 exon 55229145 55229457 55229145 ID=Msa0556980-mRNA-1:exon:16480;Parent=Msa0556980-mRNA-1
chr4_1 five_prime_UTR 55229320 55229457 55229320 ID=Msa0556980-mRNA-1:five_prime_utr;Parent=Msa0556980-mRNA-1
chr4_1 CDS 55229145 55229319 55229145 ID=Msa0556980-mRNA-1:cds;Parent=Msa0556980-mRNA-1
chr4_1 CDS 55228620 55228731 55228620 ID=Msa0556980-mRNA-1:cds;Parent=Msa0556980-mRNA-1
chr4_1 CDS 55228277 55228406 55228277 ID=Msa0556980-mRNA-1:cds;Parent=Msa0556980-mRNA-1
chr4_1 three_prime_UTR 55228022 55228276 55228022 ID=Msa0556980-mRNA-1:three_prime_utr;Parent=Msa0556980-mRNA-1
Gene Sequence

>Msa0556980

ATGGAAGTTGAACAAAGCGAAGGGTCTACCGATTCATCTTCACAAACAATGAATCATCTCTTATTTGTGGAGATGGGTGTCGGTTACGATCAACACGGGCAGAATATCACTTCTGCAGCAATGAGAGCTTGTAGGGATGCCATTTCTTCTAATTCAATCCCCGCTTTTCGCAGAGGTTCCATTCCTGGTGTCAGTTTTGGTCAAATGAAATTGAAGATCAAGCTAGGGGTTCCTCACTCTCTCCAACAATCTCTGGATATTGAGAAAGTCAAATCTGTATTTCCCTACGGGAAAATTTTGAATGTTGAAGTTGTGGATGGTGGTTTGATATGCTCAAGTGGGGTGCTTGTGGAAGAGATGGGTGACAAGAATGAGGATTGCTACATTGTGAATGCTGCTGTTTATGTCGGTTATTAA
Protein sequence

>Msa0556980

MEVEQSEGSTDSSSQTMNHLLFVEMGVGYDQHGQNITSAAMRACRDAISSNSIPAFRRGSIPGVSFGQMKLKIKLGVPHSLQQSLDIEKVKSVFPYGKILNVEVVDGGLICSSGVLVEEMGDKNEDCYIVNAAVYVGY*