AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0597330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0597330 MtrunA17_Chr4g0018611 87.859 313 10 3 1 288 1 310 0.0 502
Msa0597330 MtrunA17_Chr1g0190271 35.862 290 158 12 2 280 6 278 3.60e-32 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0597330 AT5G09380.2 36.242 298 152 11 1 286 1 272 1.01e-35 129
Msa0597330 AT4G25180.1 33.856 319 140 13 3 281 13 300 2.44e-33 124
Msa0597330 AT5G09380.1 40.000 200 98 7 97 286 29 216 4.12e-30 113
Msa0597330 AT5G09380.3 33.871 248 132 10 1 239 1 225 5.23e-21 89.7

Find 76 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTTGCTTCAATGTTTA+TGG 0.063736 4_2:+29330600 Msa0597330:three_prime_UTR
ATTCTTGATAAGATAGAATT+TGG 0.136497 4_2:+29329352 Msa0597330:intron
TTTATCATCAGCAGAAAATT+TGG 0.182176 4_2:-29328754 None:intergenic
ACTATCGGCAAAGGCGAAAA+AGG 0.239402 4_2:+29329643 Msa0597330:CDS
CATGATTCATCGCCATTATA+CGG 0.257879 4_2:+29330362 Msa0597330:three_prime_UTR
TGTAATTTGTTCTCTTGTAT+TGG 0.277119 4_2:-29330672 None:intergenic
GGAGCTTCCGAGTGGATATA+TGG 0.289404 4_2:+29329679 Msa0597330:CDS
AGTTCGTGTACGTAGCTTAT+TGG 0.293904 4_2:+29327058 Msa0597330:CDS
TACTTCTGTAATAGATGTTA+TGG 0.301982 4_2:+29328821 Msa0597330:CDS
CTTAATTCCGCAGCAGAACT+TGG 0.309638 4_2:+29329412 Msa0597330:CDS
CTACGTGGCTTATGTGAATA+GGG 0.327161 4_2:+29330400 Msa0597330:three_prime_UTR
TTCTTGATAAGATAGAATTT+GGG 0.337508 4_2:+29329353 Msa0597330:intron
TTCAAAGGTTCTCTTGTAGA+TGG 0.342994 4_2:-29330637 None:intergenic
ATCTTGATCAAGTATAGTTT+TGG 0.351285 4_2:-29330202 None:intergenic
CGCAGCAGAACTTGGGCTCT+TGG 0.353607 4_2:+29329420 Msa0597330:CDS
ACCTTTCGTGGAGTTCTGTT+TGG 0.356823 4_2:-29326774 None:intergenic
GTTTGGATCTTTCTTATCCA+TGG 0.365988 4_2:-29326757 None:intergenic
AGATCAATGGCATCAATATC+AGG 0.368015 4_2:-29330303 None:intergenic
CCTACGTGGCTTATGTGAAT+AGG 0.368086 4_2:+29330399 Msa0597330:three_prime_UTR
AGTAAAGGGAAGGAGAAAAT+TGG 0.372837 4_2:+29329611 Msa0597330:CDS
TCACTTTCACTGAGATCATT+TGG 0.383113 4_2:+29328693 Msa0597330:CDS
TCCTTCCCTTTACTGTTCAC+TGG 0.389526 4_2:-29329602 None:intergenic
AAATAGTTTGGTACCTTTCG+TGG 0.390750 4_2:-29326786 None:intergenic
AATTTGTTCTCTTGTATTGG+AGG 0.410732 4_2:-29330669 None:intergenic
TAAAACTAGTATGACATTGA+TGG 0.417630 4_2:+29330483 Msa0597330:three_prime_UTR
ATTTATGTTAATTGCTCTGT+AGG 0.419783 4_2:+29330522 Msa0597330:three_prime_UTR
CAGCTCAAGTTTATTCCAAA+GGG 0.441221 4_2:+29326884 Msa0597330:intron
AGCAACCAGTGAACAGTAAA+GGG 0.453368 4_2:+29329597 Msa0597330:CDS
GCCATTGATCTTGAATCCAT+TGG 0.463045 4_2:+29330315 Msa0597330:CDS
GGATAGAACTTACATCATAC+AGG 0.466152 4_2:-29329754 None:intergenic
AACACATTCTTCAACTGCTA+TGG 0.470804 4_2:+29328785 Msa0597330:CDS
TCTGTACTATACCCGTATAA+TGG 0.472758 4_2:-29330374 None:intergenic
AGATCATTTGGCAGTGGCAG+TGG 0.473266 4_2:+29328705 Msa0597330:CDS
AAATGCATCTCGCGCAGCAC+TGG 0.480195 4_2:-29328658 None:intergenic
TCCAATGGATTCAAGATCAA+TGG 0.494630 4_2:-29330316 None:intergenic
TTTGGCAGTGGCAGTGGTAA+TGG 0.504694 4_2:+29328711 Msa0597330:CDS
AATTGGTACGTCGAAACTAT+CGG 0.514803 4_2:+29329628 Msa0597330:CDS
AGTTTGTACTACCAATCATC+AGG 0.514839 4_2:-29330454 None:intergenic
ACTAGAGATATCCTGATGAT+TGG 0.517256 4_2:+29330443 Msa0597330:three_prime_UTR
AAGCAACCAGTGAACAGTAA+AGG 0.517583 4_2:+29329596 Msa0597330:CDS
TGGATATATGGGCAAAATGC+AGG 0.520605 4_2:+29329691 Msa0597330:CDS
TTTCTCATACAGGTATCTCC+AGG 0.521453 4_2:+29330177 Msa0597330:intron
ACAGCTCAAGTTTATTCCAA+AGG 0.522946 4_2:+29326883 Msa0597330:intron
TATATATTGCAGGAGAGGGT+TGG 0.525200 4_2:+29328277 Msa0597330:intron
AAACTACAGTGTTGTTGGGA+AGG 0.531862 4_2:+29330251 Msa0597330:CDS
GAGCTTCCGAGTGGATATAT+GGG 0.538050 4_2:+29329680 Msa0597330:CDS
TTAATTCCGCAGCAGAACTT+GGG 0.538505 4_2:+29329413 Msa0597330:CDS
ATGATTCATCGCCATTATAC+GGG 0.542869 4_2:+29330363 Msa0597330:three_prime_UTR
CACGTAGTCTTCGTCATCAG+TGG 0.543002 4_2:-29328850 None:intergenic
TTGACACAAGGCAGAAAAGT+AGG 0.543588 4_2:-29329575 None:intergenic
GACATTGATGGAATATCAAG+TGG 0.548114 4_2:+29330495 Msa0597330:three_prime_UTR
TTCACTGGTTGCTTGACACA+AGG 0.560755 4_2:-29329587 None:intergenic
AGTGAAAGTGATGAATCTCT+AGG 0.578456 4_2:-29328681 None:intergenic
ACGAAGACTACGTGGAACCA+TGG 0.578625 4_2:+29328859 Msa0597330:CDS
GAAGGTTACAGTTGACGCAA+AGG 0.581529 4_2:+29330269 Msa0597330:CDS
ACCAGTGAACAGTAAAGGGA+AGG 0.581704 4_2:+29329601 Msa0597330:CDS
CGTCTTCTTCAGCAAAGCCA+TGG 0.585581 4_2:+29326740 Msa0597330:exon
TCACTGAGATCATTTGGCAG+TGG 0.587322 4_2:+29328699 Msa0597330:CDS
GCCACTGATGTCAAATATGA+TGG 0.589781 4_2:+29329382 Msa0597330:CDS
CGAAGACTACGTGGAACCAT+GGG 0.591952 4_2:+29328860 Msa0597330:CDS
TCTTGATAAGATAGAATTTG+GGG 0.600434 4_2:+29329354 Msa0597330:intron
GATGATTGGTAGTACAAACT+AGG 0.602275 4_2:+29330457 Msa0597330:three_prime_UTR
CACTGATGACGAAGACTACG+TGG 0.626190 4_2:+29328851 Msa0597330:CDS
TCCAAACAGAACTCCACGAA+AGG 0.631732 4_2:+29326773 Msa0597330:CDS
TGATAAGATAGAATTTGGGG+AGG 0.632493 4_2:+29329357 Msa0597330:intron
TACGTCGAAACTATCGGCAA+AGG 0.632734 4_2:+29329634 Msa0597330:CDS
ACCATCATATTTGACATCAG+TGG 0.634261 4_2:-29329383 None:intergenic
TACGTGGCTTATGTGAATAG+GGG 0.643508 4_2:+29330401 Msa0597330:three_prime_UTR
CAAGAGCCCAAGTTCTGCTG+CGG 0.648219 4_2:-29329419 None:intergenic
AAAATGCAGGTCTATAAGAG+TGG 0.654564 4_2:+29329704 Msa0597330:CDS
AACTTGTTGGAGCTTCCGAG+TGG 0.660506 4_2:+29329671 Msa0597330:CDS
GATAGAACTTACATCATACA+GGG 0.671603 4_2:-29329753 None:intergenic
AGTGGTAATGGTGAAAGTAG+TGG 0.688464 4_2:+29328723 Msa0597330:CDS
CCTATTCACATAAGCCACGT+AGG 0.701429 4_2:-29330399 None:intergenic
AGCTTAAAGAAAATCAACTG+TGG 0.715988 4_2:-29329551 None:intergenic
GTATAGTACAGATACCTACG+TGG 0.820338 4_2:+29330385 Msa0597330:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTACAAATTATTTTATTA+TGG + chr4_2:29327315-29327334 Msa0597330:intron 10.0%
!!! AATTATTTTAGTTTTATTCT+TGG + chr4_2:29327395-29327414 Msa0597330:intron 10.0%
!!! TTTTTTTATATGGTAAAAAT+TGG + chr4_2:29327432-29327451 Msa0597330:intron 10.0%
!! TACTTAAAAAATTACTTAAA+AGG - chr4_2:29327626-29327645 None:intergenic 10.0%
!!! TTAATATAATTTGTGTTTCT+TGG + chr4_2:29327349-29327368 Msa0597330:intron 15.0%
!!! TAATATAATTTGTGTTTCTT+GGG + chr4_2:29327350-29327369 Msa0597330:intron 15.0%
!!! TTTAGGAGAATTTTTTTATA+TGG + chr4_2:29327422-29327441 Msa0597330:intron 15.0%
!! AATTTGACATAAGAAATTTA+TGG - chr4_2:29327572-29327591 None:intergenic 15.0%
!!! ACATTGTTTTTTTTCAAATT+TGG + chr4_2:29327595-29327614 Msa0597330:intron 15.0%
!! TAACATGTTAAATATTAGTT+TGG + chr4_2:29327821-29327840 Msa0597330:intron 15.0%
!! TAGAATATAATAACAAGATA+TGG - chr4_2:29328063-29328082 None:intergenic 15.0%
!! TACTAAAATATATCTAAAAG+TGG + chr4_2:29328183-29328202 Msa0597330:intron 15.0%
!! CAATATATAGATAAAACAAA+TGG - chr4_2:29328266-29328285 None:intergenic 15.0%
!!! ATATGTGTTGATTTTTAATA+TGG + chr4_2:29329324-29329343 Msa0597330:intron 15.0%
!! AGTTAAAAATGGTATAAAAA+GGG - chr4_2:29330124-29330143 None:intergenic 15.0%
!! AAGTTAAAAATGGTATAAAA+AGG - chr4_2:29330125-29330144 None:intergenic 15.0%
!! TCAATAATCAAAGTTAAAAA+TGG - chr4_2:29330135-29330154 None:intergenic 15.0%
!!! GTTTTATTCTTGGTTAATTT+AGG + chr4_2:29327405-29327424 Msa0597330:intron 20.0%
!! AAAGTGAAATCTTTATATCT+TGG - chr4_2:29327505-29327524 None:intergenic 20.0%
!!! AGATATAAAGATTTCACTTT+TGG + chr4_2:29327505-29327524 Msa0597330:intron 20.0%
!!! CAGGTAATTGAAATTTTTTA+AGG + chr4_2:29327537-29327556 Msa0597330:intron 20.0%
!!! TTTGCTGTTGATTAAATTTT+CGG - chr4_2:29327684-29327703 None:intergenic 20.0%
!!! AAATCGTGAATACATTTTTA+CGG - chr4_2:29327888-29327907 None:intergenic 20.0%
!! TATAATACTATGATAGCATT+TGG - chr4_2:29328154-29328173 None:intergenic 20.0%
!!! TTGTTTTATCTATATATTGC+AGG + chr4_2:29328267-29328286 Msa0597330:intron 20.0%
!! TATTTCATAATTGTGATGTT+GGG + chr4_2:29328388-29328407 Msa0597330:intron 20.0%
!! AAACTTCTTTGTAAAGAAAA+TGG - chr4_2:29328477-29328496 None:intergenic 20.0%
!!! ATTTTTGCTAATGTCTATAT+AGG - chr4_2:29328506-29328525 None:intergenic 20.0%
!!! CAAAACAAATAAAGCTTTTA+AGG + chr4_2:29329046-29329065 Msa0597330:intron 20.0%
!! ATTCTTGATAAGATAGAATT+TGG + chr4_2:29329352-29329371 Msa0597330:intron 20.0%
!! TTCTTGATAAGATAGAATTT+GGG + chr4_2:29329353-29329372 Msa0597330:intron 20.0%
!!! TACATAGAAAATTTTTCTTC+AGG + chr4_2:29329514-29329533 Msa0597330:intron 20.0%
!!! TTATTGTCAATTTTGAAACA+TGG - chr4_2:29329778-29329797 None:intergenic 20.0%
!! TACTTCATTCAGTAAATATA+TGG - chr4_2:29329823-29329842 None:intergenic 20.0%
!!! AAATATGGGATAACTTTTTT+GGG - chr4_2:29329906-29329925 None:intergenic 20.0%
!!! AAAATATGGGATAACTTTTT+TGG - chr4_2:29329907-29329926 None:intergenic 20.0%
!!! AAAAAAGTTATCCCATATTT+TGG + chr4_2:29329906-29329925 Msa0597330:intron 20.0%
!! TGGTATAATAACCAAAATAT+GGG - chr4_2:29329920-29329939 None:intergenic 20.0%
!! ATGGTATAATAACCAAAATA+TGG - chr4_2:29329921-29329940 None:intergenic 20.0%
!!! TTATACCATTTTTAACTTTG+AGG + chr4_2:29329932-29329951 Msa0597330:intron 20.0%
!!! TATACCATTTTTAACTTTGA+GGG + chr4_2:29329933-29329952 Msa0597330:intron 20.0%
! AAGTAGGGAAAAAAATAGTT+TGG - chr4_2:29326801-29326820 None:intergenic 25.0%
!!! ATTTTCGTTGTTTGAGATTT+GGG - chr4_2:29326914-29326933 None:intergenic 25.0%
!!! CGAAAGTGTTGAATAAAAAT+CGG + chr4_2:29327242-29327261 Msa0597330:intron 25.0%
! TTGGTTAAAAATTAGCTAGT+CGG - chr4_2:29327486-29327505 None:intergenic 25.0%
! TTTAATCAACAGCAAAAACA+TGG + chr4_2:29327688-29327707 Msa0597330:intron 25.0%
! TAACTCGCTATAATAGATTT+AGG + chr4_2:29327855-29327874 Msa0597330:intron 25.0%
! AACTCGCTATAATAGATTTA+GGG + chr4_2:29327856-29327875 Msa0597330:intron 25.0%
!! AATCGTGAATACATTTTTAC+GGG - chr4_2:29327887-29327906 None:intergenic 25.0%
!! ATGTAGCTTCTAAAAAATCA+TGG - chr4_2:29327979-29327998 None:intergenic 25.0%
!!! TTTTAGAAGCTACATTGTAT+AGG + chr4_2:29327984-29328003 Msa0597330:intron 25.0%
! ACACTGTACAAATATATTCA+GGG - chr4_2:29328020-29328039 None:intergenic 25.0%
!!! TTACATTGTTTTGCAACTTT+AGG - chr4_2:29328313-29328332 None:intergenic 25.0%
! AAGTTGCAAAACAATGTAAT+CGG + chr4_2:29328314-29328333 Msa0597330:intron 25.0%
!! ATGTCTTTTGACATATAAAG+TGG - chr4_2:29328346-29328365 None:intergenic 25.0%
! CTATTTCATAATTGTGATGT+TGG + chr4_2:29328387-29328406 Msa0597330:intron 25.0%
!! TAGCAAAAATCATTTTACCA+TGG + chr4_2:29328516-29328535 Msa0597330:intron 25.0%
!!! GTCAAAGTAATTTTTTTCCA+TGG - chr4_2:29328536-29328555 None:intergenic 25.0%
! AGAATATCTTAGATTCTATG+TGG + chr4_2:29328563-29328582 Msa0597330:intron 25.0%
!!! ATTCTAAAACACATTCCTTT+TGG + chr4_2:29328619-29328638 Msa0597330:intron 25.0%
! TTTATCATCAGCAGAAAATT+TGG - chr4_2:29328757-29328776 None:intergenic 25.0%
!! TTTTCTGCTGATGATAAATA+TGG + chr4_2:29328759-29328778 Msa0597330:CDS 25.0%
! TACTTCTGTAATAGATGTTA+TGG + chr4_2:29328821-29328840 Msa0597330:CDS 25.0%
!!! TGTTTCTAACACTTTTTTCT+AGG + chr4_2:29329247-29329266 Msa0597330:intron 25.0%
!!! GTTTCTAACACTTTTTTCTA+GGG + chr4_2:29329248-29329267 Msa0597330:intron 25.0%
! TCTTGATAAGATAGAATTTG+GGG + chr4_2:29329354-29329373 Msa0597330:intron 25.0%
! TAAATATATGGTAAATGAGG+TGG - chr4_2:29329811-29329830 None:intergenic 25.0%
! CAGTAAATATATGGTAAATG+AGG - chr4_2:29329814-29329833 None:intergenic 25.0%
! GAATCACAATTAAACTAACT+TGG - chr4_2:29329846-29329865 None:intergenic 25.0%
!!! ATACCATTTTTAACTTTGAG+GGG + chr4_2:29329934-29329953 Msa0597330:intron 25.0%
!!! ATTAGTTTTGTTTCTCATAC+AGG + chr4_2:29330167-29330186 Msa0597330:intron 25.0%
!! ATCTTGATCAAGTATAGTTT+TGG - chr4_2:29330205-29330224 None:intergenic 25.0%
! TAAAACTAGTATGACATTGA+TGG + chr4_2:29330483-29330502 Msa0597330:three_prime_UTR 25.0%
! ATTTATGTTAATTGCTCTGT+AGG + chr4_2:29330522-29330541 Msa0597330:three_prime_UTR 25.0%
! TTCATTTGCTTCAATGTTTA+TGG + chr4_2:29330600-29330619 Msa0597330:three_prime_UTR 25.0%
!!! TGTAATTTGTTCTCTTGTAT+TGG - chr4_2:29330675-29330694 None:intergenic 25.0%
!!! AAAAGAAAAGCACTTTTCTG+AGG - chr4_2:29326833-29326852 None:intergenic 30.0%
!! CATTTTCGTTGTTTGAGATT+TGG - chr4_2:29326915-29326934 None:intergenic 30.0%
ATTATCTCACTAACTTTCTC+AGG + chr4_2:29327769-29327788 Msa0597330:intron 30.0%
ACTCGCTATAATAGATTTAG+GGG + chr4_2:29327857-29327876 Msa0597330:intron 30.0%
TAAGAACTTCAAACTCATTG+TGG - chr4_2:29327954-29327973 None:intergenic 30.0%
CACACTGTACAAATATATTC+AGG - chr4_2:29328021-29328040 None:intergenic 30.0%
ATACTATGATAGCATTTGGT+AGG - chr4_2:29328150-29328169 None:intergenic 30.0%
TTATCTATATATTGCAGGAG+AGG + chr4_2:29328272-29328291 Msa0597330:intron 30.0%
TATCTATATATTGCAGGAGA+GGG + chr4_2:29328273-29328292 Msa0597330:intron 30.0%
! GGGAAGGAAAGATAATATTT+TGG + chr4_2:29328408-29328427 Msa0597330:intron 30.0%
!!! TGCAAGTTTTAAACACCAAA+AGG - chr4_2:29328637-29328656 None:intergenic 30.0%
! TGAACCTTTGATTTAACTAC+AGG + chr4_2:29328947-29328966 Msa0597330:intron 30.0%
!!! TTTGTTTTGTTCATCAAACC+TGG - chr4_2:29329035-29329054 None:intergenic 30.0%
TTGCATGAAATTCATCCAAT+AGG - chr4_2:29329127-29329146 None:intergenic 30.0%
CCAAATAGAATAGAGAATGT+CGG + chr4_2:29329204-29329223 Msa0597330:intron 30.0%
GGATATATATTAGCAATAGC+CGG + chr4_2:29329269-29329288 Msa0597330:intron 30.0%
AACCAAATATTACATGGAAC+CGG - chr4_2:29329291-29329310 None:intergenic 30.0%
TGGCAAAACCAAATATTACA+TGG - chr4_2:29329297-29329316 None:intergenic 30.0%
AATCAACACATATCTGTTAG+TGG - chr4_2:29329317-29329336 None:intergenic 30.0%
AGCTTAAAGAAAATCAACTG+TGG - chr4_2:29329554-29329573 None:intergenic 30.0%
GGAGCAGTCAAATTAAAAAT+AGG + chr4_2:29329725-29329744 Msa0597330:CDS 30.0%
GATAGAACTTACATCATACA+GGG - chr4_2:29329756-29329775 None:intergenic 30.0%
TTACCCCTCAAAGTTAAAAA+TGG - chr4_2:29329940-29329959 None:intergenic 30.0%
AAGAAAAACTACAGTGTTGT+TGG + chr4_2:29330246-29330265 Msa0597330:CDS 30.0%
! AGAAAAACTACAGTGTTGTT+GGG + chr4_2:29330247-29330266 Msa0597330:CDS 30.0%
! TTGAGTCTTTTATAGTCCAA+TGG - chr4_2:29330334-29330353 None:intergenic 30.0%
! AATTTGTTCTCTTGTATTGG+AGG - chr4_2:29330672-29330691 None:intergenic 30.0%
GTTTGGATCTTTCTTATCCA+TGG - chr4_2:29326760-29326779 None:intergenic 35.0%
AAATAGTTTGGTACCTTTCG+TGG - chr4_2:29326789-29326808 None:intergenic 35.0%
ACAGCTCAAGTTTATTCCAA+AGG + chr4_2:29326883-29326902 Msa0597330:intron 35.0%
CAGCTCAAGTTTATTCCAAA+GGG + chr4_2:29326884-29326903 Msa0597330:intron 35.0%
!! TTGTTTTGTTACAGTGAAGC+TGG + chr4_2:29327008-29327027 Msa0597330:intron 35.0%
GGTAAAAATTGGATGTGTGT+TGG + chr4_2:29327443-29327462 Msa0597330:intron 35.0%
!! TCACTTTTGGATTTGTGAAC+AGG + chr4_2:29327518-29327537 Msa0597330:intron 35.0%
ATCAACAGCAAAAACATGGT+AGG + chr4_2:29327692-29327711 Msa0597330:intron 35.0%
!!! AACATGGTAGGTTTTGAAAG+AGG + chr4_2:29327704-29327723 Msa0597330:intron 35.0%
!! GAAAGAGGCATTTATTCACA+TGG + chr4_2:29327719-29327738 Msa0597330:intron 35.0%
!! ATTGTTTTGCAACTTTAGGC+TGG - chr4_2:29328309-29328328 None:intergenic 35.0%
TCATAATTGTGATGTTGGGA+AGG + chr4_2:29328392-29328411 Msa0597330:intron 35.0%
AGTGAAAGTGATGAATCTCT+AGG - chr4_2:29328684-29328703 None:intergenic 35.0%
TCACTTTCACTGAGATCATT+TGG + chr4_2:29328693-29328712 Msa0597330:CDS 35.0%
AACACATTCTTCAACTGCTA+TGG + chr4_2:29328785-29328804 Msa0597330:CDS 35.0%
CAAAGGTTCAGATGCTTATA+TGG - chr4_2:29328937-29328956 None:intergenic 35.0%
GGATCCTGTAGTTAAATCAA+AGG - chr4_2:29328954-29328973 None:intergenic 35.0%
TCCAATAGGTGATAGTTTGA+CGG - chr4_2:29329113-29329132 None:intergenic 35.0%
CCGACATTCTCTATTCTATT+TGG - chr4_2:29329207-29329226 None:intergenic 35.0%
TGATAAGATAGAATTTGGGG+AGG + chr4_2:29329357-29329376 Msa0597330:intron 35.0%
ACCATCATATTTGACATCAG+TGG - chr4_2:29329386-29329405 None:intergenic 35.0%
TTCAGCTGCATAATGATAGT+AGG - chr4_2:29329479-29329498 None:intergenic 35.0%
AGTAAAGGGAAGGAGAAAAT+TGG + chr4_2:29329611-29329630 Msa0597330:CDS 35.0%
AATTGGTACGTCGAAACTAT+CGG + chr4_2:29329628-29329647 Msa0597330:CDS 35.0%
! ATTTTGCCCATATATCCACT+CGG - chr4_2:29329689-29329708 None:intergenic 35.0%
AAAATGCAGGTCTATAAGAG+TGG + chr4_2:29329704-29329723 Msa0597330:CDS 35.0%
GGATAGAACTTACATCATAC+AGG - chr4_2:29329757-29329776 None:intergenic 35.0%
AAGTCCTGTAAAACAAGGTA+AGG + chr4_2:29330012-29330031 Msa0597330:intron 35.0%
!!! TCAAGTATAGTTTTGGTACC+TGG - chr4_2:29330198-29330217 None:intergenic 35.0%
AGATCAATGGCATCAATATC+AGG - chr4_2:29330306-29330325 None:intergenic 35.0%
! TCCAATGGATTCAAGATCAA+TGG - chr4_2:29330319-29330338 None:intergenic 35.0%
CATGATTCATCGCCATTATA+CGG + chr4_2:29330362-29330381 Msa0597330:three_prime_UTR 35.0%
ATGATTCATCGCCATTATAC+GGG + chr4_2:29330363-29330382 Msa0597330:three_prime_UTR 35.0%
TCTGTACTATACCCGTATAA+TGG - chr4_2:29330377-29330396 None:intergenic 35.0%
ACTAGAGATATCCTGATGAT+TGG + chr4_2:29330443-29330462 Msa0597330:three_prime_UTR 35.0%
AGTTTGTACTACCAATCATC+AGG - chr4_2:29330457-29330476 None:intergenic 35.0%
! GATGATTGGTAGTACAAACT+AGG + chr4_2:29330457-29330476 Msa0597330:three_prime_UTR 35.0%
GACATTGATGGAATATCAAG+TGG + chr4_2:29330495-29330514 Msa0597330:three_prime_UTR 35.0%
TTCAAAGGTTCTCTTGTAGA+TGG - chr4_2:29330640-29330659 None:intergenic 35.0%
!! TGAGATTTGGGTTTTCCCTT+TGG - chr4_2:29326902-29326921 None:intergenic 40.0%
AGTTCGTGTACGTAGCTTAT+TGG + chr4_2:29327058-29327077 Msa0597330:CDS 40.0%
! GAGGCATTTATTCACATGGT+AGG + chr4_2:29327723-29327742 Msa0597330:intron 40.0%
TTCACATGGTAGGTTACAGT+AGG + chr4_2:29327733-29327752 Msa0597330:intron 40.0%
! GGTAGGTTACAGTAGGTTTT+TGG + chr4_2:29327740-29327759 Msa0597330:intron 40.0%
ATGATAGCATTTGGTAGGAC+AGG - chr4_2:29328145-29328164 None:intergenic 40.0%
TATATATTGCAGGAGAGGGT+TGG + chr4_2:29328277-29328296 Msa0597330:intron 40.0%
TTTATCATTGTGACCAGAGC+AGG - chr4_2:29328589-29328608 None:intergenic 40.0%
!! AGTGGTAATGGTGAAAGTAG+TGG + chr4_2:29328723-29328742 Msa0597330:CDS 40.0%
GGATAAGAATTTCTCACCCA+TGG - chr4_2:29328879-29328898 None:intergenic 40.0%
GATAGGAAGTGAACATCTGA+GGG - chr4_2:29328900-29328919 None:intergenic 40.0%
AGATAGGAAGTGAACATCTG+AGG - chr4_2:29328901-29328920 None:intergenic 40.0%
TGGATGAGAAAGATGCAGAT+AGG - chr4_2:29328917-29328936 None:intergenic 40.0%
! GGATAGTTTGAGTTTGTGTC+AGG - chr4_2:29328975-29328994 None:intergenic 40.0%
ATCCTACTACTCTTCCTCTA+AGG + chr4_2:29328991-29329010 Msa0597330:intron 40.0%
TTCCTTAGAGGAAGAGTAGT+AGG - chr4_2:29328996-29329015 None:intergenic 40.0%
AAACCTGGATCTCCAGAATT+GGG - chr4_2:29329020-29329039 None:intergenic 40.0%
TCCGTCAAACTATCACCTAT+TGG + chr4_2:29329109-29329128 Msa0597330:intron 40.0%
! AGCCGGTTCCATGTAATATT+TGG + chr4_2:29329286-29329305 Msa0597330:intron 40.0%
GCCACTGATGTCAAATATGA+TGG + chr4_2:29329382-29329401 Msa0597330:CDS 40.0%
TTAATTCCGCAGCAGAACTT+GGG + chr4_2:29329413-29329432 Msa0597330:CDS 40.0%
TTGACACAAGGCAGAAAAGT+AGG - chr4_2:29329578-29329597 None:intergenic 40.0%
AAGCAACCAGTGAACAGTAA+AGG + chr4_2:29329596-29329615 Msa0597330:CDS 40.0%
AGCAACCAGTGAACAGTAAA+GGG + chr4_2:29329597-29329616 Msa0597330:CDS 40.0%
! GAAAAAGGCGAGTAACTTGT+TGG + chr4_2:29329658-29329677 Msa0597330:CDS 40.0%
TGGATATATGGGCAAAATGC+AGG + chr4_2:29329691-29329710 Msa0597330:CDS 40.0%
! TTAACTTTGAGGGGTAACCT+TGG + chr4_2:29329943-29329962 Msa0597330:intron 40.0%
GGTTCAAGTCCTGTAAAACA+AGG + chr4_2:29330007-29330026 Msa0597330:intron 40.0%
CGTACAATACACCAAATGGT+GGG + chr4_2:29330038-29330057 Msa0597330:intron 40.0%
ATGGTATAAAAAGGGCAACC+TGG - chr4_2:29330116-29330135 None:intergenic 40.0%
TTTCTCATACAGGTATCTCC+AGG + chr4_2:29330177-29330196 Msa0597330:intron 40.0%
! AAACTACAGTGTTGTTGGGA+AGG + chr4_2:29330251-29330270 Msa0597330:CDS 40.0%
GCCATTGATCTTGAATCCAT+TGG + chr4_2:29330315-29330334 Msa0597330:CDS 40.0%
GTATAGTACAGATACCTACG+TGG + chr4_2:29330385-29330404 Msa0597330:three_prime_UTR 40.0%
CTACGTGGCTTATGTGAATA+GGG + chr4_2:29330400-29330419 Msa0597330:three_prime_UTR 40.0%
TACGTGGCTTATGTGAATAG+GGG + chr4_2:29330401-29330420 Msa0597330:three_prime_UTR 40.0%
! TGGAGGCTAGAGATTTTCAA+AGG - chr4_2:29330655-29330674 None:intergenic 40.0%
TCCAAACAGAACTCCACGAA+AGG + chr4_2:29326773-29326792 Msa0597330:CDS 45.0%
ACCTTTCGTGGAGTTCTGTT+TGG - chr4_2:29326777-29326796 None:intergenic 45.0%
CTGAGGAACACGATGAAGTA+GGG - chr4_2:29326816-29326835 None:intergenic 45.0%
TCTGAGGAACACGATGAAGT+AGG - chr4_2:29326817-29326836 None:intergenic 45.0%
TCACTGAGATCATTTGGCAG+TGG + chr4_2:29328699-29328718 Msa0597330:CDS 45.0%
CAAACCTGGATCTCCAGAAT+TGG - chr4_2:29329021-29329040 None:intergenic 45.0%
ATGGGTCCAACATAAAGTGG+CGG - chr4_2:29329153-29329172 None:intergenic 45.0%
GGAATGGGTCCAACATAAAG+TGG - chr4_2:29329156-29329175 None:intergenic 45.0%
TGTTGGACCCATTCCCAAAA+CGG + chr4_2:29329161-29329180 Msa0597330:intron 45.0%
CTTAATTCCGCAGCAGAACT+TGG + chr4_2:29329412-29329431 Msa0597330:CDS 45.0%
! TTCACTGGTTGCTTGACACA+AGG - chr4_2:29329590-29329609 None:intergenic 45.0%
ACCAGTGAACAGTAAAGGGA+AGG + chr4_2:29329601-29329620 Msa0597330:CDS 45.0%
TCCTTCCCTTTACTGTTCAC+TGG - chr4_2:29329605-29329624 None:intergenic 45.0%
TACGTCGAAACTATCGGCAA+AGG + chr4_2:29329634-29329653 Msa0597330:CDS 45.0%
ACTATCGGCAAAGGCGAAAA+AGG + chr4_2:29329643-29329662 Msa0597330:CDS 45.0%
GAGCTTCCGAGTGGATATAT+GGG + chr4_2:29329680-29329699 Msa0597330:CDS 45.0%
CAACTTTACCAGTTGTGCCA+AGG - chr4_2:29329963-29329982 None:intergenic 45.0%
GTTGTTGTCATGTGCCTGAA+AGG + chr4_2:29329979-29329998 Msa0597330:intron 45.0%
!!! GCAGCCTTACCTTGTTTTAC+AGG - chr4_2:29330019-29330038 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + chr4_2:29330034-29330053 Msa0597330:intron 45.0%
GCGTACAATACACCAAATGG+TGG + chr4_2:29330037-29330056 Msa0597330:intron 45.0%
GAAGGTTACAGTTGACGCAA+AGG + chr4_2:29330269-29330288 Msa0597330:CDS 45.0%
CCTATTCACATAAGCCACGT+AGG - chr4_2:29330402-29330421 None:intergenic 45.0%
CCTACGTGGCTTATGTGAAT+AGG + chr4_2:29330399-29330418 Msa0597330:three_prime_UTR 45.0%
!! AAAAAAAATTGAAAAAAAAA+AGG - chr4_2:29326698-29326717 None:intergenic 5.0%
CGTCTTCTTCAGCAAAGCCA+TGG + chr4_2:29326740-29326759 Msa0597330:exon 50.0%
AGATTCTATGTGGCCTGCTC+TGG + chr4_2:29328573-29328592 Msa0597330:intron 50.0%
AGATCATTTGGCAGTGGCAG+TGG + chr4_2:29328705-29328724 Msa0597330:CDS 50.0%
TTTGGCAGTGGCAGTGGTAA+TGG + chr4_2:29328711-29328730 Msa0597330:CDS 50.0%
CACGTAGTCTTCGTCATCAG+TGG - chr4_2:29328853-29328872 None:intergenic 50.0%
! CACTGATGACGAAGACTACG+TGG + chr4_2:29328851-29328870 Msa0597330:CDS 50.0%
ACGAAGACTACGTGGAACCA+TGG + chr4_2:29328859-29328878 Msa0597330:CDS 50.0%
CGAAGACTACGTGGAACCAT+GGG + chr4_2:29328860-29328879 Msa0597330:CDS 50.0%
CCAGAATTGGGCTTCCTTAG+AGG - chr4_2:29329008-29329027 None:intergenic 50.0%
! CCTCTAAGGAAGCCCAATTC+TGG + chr4_2:29329005-29329024 Msa0597330:intron 50.0%
! AAGCCCAATTCTGGAGATCC+AGG + chr4_2:29329014-29329033 Msa0597330:intron 50.0%
! CAAGTCCCGCCACTTTATGT+TGG + chr4_2:29329144-29329163 Msa0597330:intron 50.0%
TGGGTCCAACATAAAGTGGC+GGG - chr4_2:29329152-29329171 None:intergenic 50.0%
!!! TGTTCCGCCGTTTTGGGAAT+GGG - chr4_2:29329171-29329190 None:intergenic 50.0%
!!! ATGTTCCGCCGTTTTGGGAA+TGG - chr4_2:29329172-29329191 None:intergenic 50.0%
!! CGAGTATGTTCCGCCGTTTT+GGG - chr4_2:29329177-29329196 None:intergenic 50.0%
! TCGAGTATGTTCCGCCGTTT+TGG - chr4_2:29329178-29329197 None:intergenic 50.0%
!! AACTTGTTGGAGCTTCCGAG+TGG + chr4_2:29329671-29329690 Msa0597330:CDS 50.0%
GGAGCTTCCGAGTGGATATA+TGG + chr4_2:29329679-29329698 Msa0597330:CDS 50.0%
GTCATGTGCCTGAAAGGTCA+CGG + chr4_2:29329985-29330004 Msa0597330:intron 50.0%
TCATGTGCCTGAAAGGTCAC+GGG + chr4_2:29329986-29330005 Msa0597330:intron 50.0%
ACTTGAACCCGTGACCTTTC+AGG - chr4_2:29329996-29330015 None:intergenic 50.0%
AAATGCATCTCGCGCAGCAC+TGG - chr4_2:29328661-29328680 None:intergenic 55.0%
TGGACCCATTCCCAAAACGG+CGG + chr4_2:29329164-29329183 Msa0597330:intron 55.0%
CAAGAGCCCAAGTTCTGCTG+CGG - chr4_2:29329422-29329441 None:intergenic 55.0%
AGGGGTAACCTTGGCACAAC+TGG + chr4_2:29329952-29329971 Msa0597330:intron 55.0%
CTAGAGCTCCCGCATATGCA+GGG - chr4_2:29330077-29330096 None:intergenic 55.0%
ACTAGAGCTCCCGCATATGC+AGG - chr4_2:29330078-29330097 None:intergenic 55.0%
! TGCATATGCGGGAGCTCTAG+TGG + chr4_2:29330077-29330096 Msa0597330:intron 55.0%
CGCAGCAGAACTTGGGCTCT+TGG + chr4_2:29329420-29329439 Msa0597330:CDS 60.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr4_2:29330050-29330069 Msa0597330:intron 60.0%
CATATGCAGGGTCCGGGAAG+GGG - chr4_2:29330065-29330084 None:intergenic 60.0%
GCATATGCAGGGTCCGGGAA+GGG - chr4_2:29330066-29330085 None:intergenic 60.0%
CTTCCCGGACCCTGCATATG+CGG + chr4_2:29330065-29330084 Msa0597330:intron 60.0%
TTCCCGGACCCTGCATATGC+GGG + chr4_2:29330066-29330085 Msa0597330:intron 60.0%
AGTGGACGCTCTAGTGCACC+AGG + chr4_2:29330095-29330114 Msa0597330:intron 60.0%
CGGGAAGGGGTCCCACCATT+TGG - chr4_2:29330052-29330071 None:intergenic 65.0%
CGCATATGCAGGGTCCGGGA+AGG - chr4_2:29330067-29330086 None:intergenic 65.0%
CTCCCGCATATGCAGGGTCC+GGG - chr4_2:29330071-29330090 None:intergenic 65.0%
GCTCCCGCATATGCAGGGTC+CGG - chr4_2:29330072-29330091 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 29326678 29330694 29326678 ID=Msa0597330;Name=Msa0597330
chr4_2 mRNA 29326678 29330694 29326678 ID=Msa0597330-mRNA-1;Parent=Msa0597330;Name=Msa0597330-mRNA-1;_AED=0.15;_eAED=0.15;_QI=81|0.85|0.87|1|1|1|8|350|288
chr4_2 exon 29326678 29326794 29326678 ID=Msa0597330-mRNA-1:exon:7458;Parent=Msa0597330-mRNA-1
chr4_2 exon 29326887 29326933 29326887 ID=Msa0597330-mRNA-1:exon:7459;Parent=Msa0597330-mRNA-1
chr4_2 exon 29327022 29327079 29327022 ID=Msa0597330-mRNA-1:exon:7460;Parent=Msa0597330-mRNA-1
chr4_2 exon 29328289 29328328 29328289 ID=Msa0597330-mRNA-1:exon:7461;Parent=Msa0597330-mRNA-1
chr4_2 exon 29328658 29328881 29328658 ID=Msa0597330-mRNA-1:exon:7462;Parent=Msa0597330-mRNA-1
chr4_2 exon 29329364 29329441 29329364 ID=Msa0597330-mRNA-1:exon:7463;Parent=Msa0597330-mRNA-1
chr4_2 exon 29329536 29329763 29329536 ID=Msa0597330-mRNA-1:exon:7464;Parent=Msa0597330-mRNA-1
chr4_2 exon 29330189 29330694 29330189 ID=Msa0597330-mRNA-1:exon:7465;Parent=Msa0597330-mRNA-1
chr4_2 five_prime_UTR 29326678 29326758 29326678 ID=Msa0597330-mRNA-1:five_prime_utr;Parent=Msa0597330-mRNA-1
chr4_2 CDS 29326759 29326794 29326759 ID=Msa0597330-mRNA-1:cds;Parent=Msa0597330-mRNA-1
chr4_2 CDS 29326887 29326933 29326887 ID=Msa0597330-mRNA-1:cds;Parent=Msa0597330-mRNA-1
chr4_2 CDS 29327022 29327079 29327022 ID=Msa0597330-mRNA-1:cds;Parent=Msa0597330-mRNA-1
chr4_2 CDS 29328289 29328328 29328289 ID=Msa0597330-mRNA-1:cds;Parent=Msa0597330-mRNA-1
chr4_2 CDS 29328658 29328881 29328658 ID=Msa0597330-mRNA-1:cds;Parent=Msa0597330-mRNA-1
chr4_2 CDS 29329364 29329441 29329364 ID=Msa0597330-mRNA-1:cds;Parent=Msa0597330-mRNA-1
chr4_2 CDS 29329536 29329763 29329536 ID=Msa0597330-mRNA-1:cds;Parent=Msa0597330-mRNA-1
chr4_2 CDS 29330189 29330344 29330189 ID=Msa0597330-mRNA-1:cds;Parent=Msa0597330-mRNA-1
chr4_2 three_prime_UTR 29330345 29330694 29330345 ID=Msa0597330-mRNA-1:three_prime_utr;Parent=Msa0597330-mRNA-1
Gene Sequence

>Msa0597330

ATGGATAAGAAAGATCCAAACAGAACTCCACGAAAGCTCAAGTTTATTCCAAAGGGAAAACCCAAATCTCAAACAACGAAAATTGAAGCTGGTGTTGAAGATGAACAGAAGAATTCTGAAGTTCGTGTACGTAGCTTATTGGAGAGGGTTGGAAGACGCCAGCCTAAAGTTGCAAAACAATCCAGTGCTGCGCGAGATGCATTTCCTAGAGATTCATCACTTTCACTGAGATCATTTGGCAGTGGCAGTGGTAATGGTGAAAGTAGTGGCTCAGAATCCAAATTTTCTGCTGATGATAAATATGGTTCAACACATTCTTCAACTGCTATGGACTATCAGAGTTATACTTCTGTAATAGATGTTATGGATGACTCCACTGATGACGAAGACTACGTGGAACCATGGATAGAATTTGGGGAGGCTGCCACTGATGTCAAATATGATGGTGATAATCTTAATTCCGCAGCAGAACTTGGGCTCTTGGACAAAAGTGAGCAACCACAGTTGATTTTCTTTAAGCTTCCTACTTTTCTGCCTTGTGTCAAGCAACCAGTGAACAGTAAAGGGAAGGAGAAAATTGGTACGTCGAAACTATCGGCAAAGGCGAAAAAGGCGAGTAACTTGTTGGAGCTTCCGAGTGGATATATGGGCAAAATGCAGGTCTATAAGAGTGGAGCAGTCAAATTAAAAATAGGAGAAACCCTGTATGATGTATCTCCAGGTACCAAAACTATACTTGATCAAGATGTTGTAGCAATTAACACTGCAAAGAAAAACTACAGTGTTGTTGGGAAGGTTACAGTTGACGCAAAGGCTGTTGTAACTCCTGATATTGATGCCATTGATCTTGAATCCATTGGACTATAA
Protein sequence

>Msa0597330

MDKKDPNRTPRKLKFIPKGKPKSQTTKIEAGVEDEQKNSEVRVRSLLERVGRRQPKVAKQSSAARDAFPRDSSLSLRSFGSGSGNGESSGSESKFSADDKYGSTHSSTAMDYQSYTSVIDVMDDSTDDEDYVEPWIEFGEAATDVKYDGDNLNSAAELGLLDKSEQPQLIFFKLPTFLPCVKQPVNSKGKEKIGTSKLSAKAKKASNLLELPSGYMGKMQVYKSGAVKLKIGETLYDVSPGTKTILDQDVVAINTAKKNYSVVGKVTVDAKAVVTPDIDAIDLESIGL*