AlfalfaGEDB | Msa0534900

M. sativa cultivar zhongmu-4 / Msa0534900

KEGG: -
GO: -
PFAM: PF00582.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534900	XP_013454933.1	98.780	164	2	0	1	164	1	164	8.56e-115	332

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534900	B7FGP5	98.780	164	2	0	1	164	1	164	4.09e-115	332

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003640	Msa0534900	0.834470	2.942906e-56	-8.615850e-47
Msa0004840	Msa0534900	0.806091	9.731758e-50	-8.615850e-47
Msa0006040	Msa0534900	0.802312	5.954777e-49	-8.615850e-47
Msa0006550	Msa0534900	0.814774	1.297939e-51	-8.615850e-47
Msa0007850	Msa0534900	0.813148	2.963790e-51	-8.615850e-47
Msa0008840	Msa0534900	0.809018	2.328258e-50	-8.615850e-47
Msa0010490	Msa0534900	0.853325	2.575633e-61	-8.615850e-47
Msa0010500	Msa0534900	0.846335	2.317712e-59	-8.615850e-47
Msa0011960	Msa0534900	0.818641	1.762833e-52	-8.615850e-47
Msa0015930	Msa0534900	0.817024	4.086948e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534900	MtrunA17_Chr4g0008801	98.780	164	2	0	1	164	1	164	7.87e-119	332
Msa0534900	MtrunA17_Chr7g0240701	70.701	157	46	0	1	157	1	157	1.94e-83	243
Msa0534900	MtrunA17_Chr4g0032261	65.409	159	55	0	1	159	1	159	5.69e-75	221
Msa0534900	MtrunA17_Chr5g0433951	56.098	164	72	0	1	164	1	164	1.12e-61	187
Msa0534900	MtrunA17_Chr2g0294421	47.500	160	84	0	1	160	1	160	1.30e-48	154
Msa0534900	MtrunA17_Chr5g0427291	37.654	162	85	5	9	157	12	170	1.36e-21	86.3
Msa0534900	MtrunA17_Chr1g0194801	33.742	163	90	6	3	157	4	156	1.10e-17	75.5
Msa0534900	MtrunA17_Chr1g0192651	32.515	163	100	4	5	165	9	163	5.42e-17	73.9
Msa0534900	MtrunA17_Chr6g0464761	73.171	41	11	0	95	135	12	52	1.06e-15	68.2
Msa0534900	MtrunA17_Chr4g0001641	48.387	62	32	0	100	161	8	69	1.14e-15	67.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0534900	AT3G53990.1	75.472	159	39	0	1	159	1	159	2.22e-88	255
Msa0534900	AT3G03270.2	60.377	159	63	0	1	159	1	159	4.54e-63	191
Msa0534900	AT3G17020.1	50.000	154	76	1	5	157	7	160	9.72e-55	170
Msa0534900	AT3G53990.2	74.490	98	25	0	1	98	1	98	1.75e-47	150
Msa0534900	AT3G03270.1	57.037	135	58	0	1	135	1	135	8.14e-47	151
Msa0534900	AT3G17020.2	47.253	91	47	1	5	94	7	97	5.99e-25	93.2
Msa0534900	AT1G09740.2	32.530	166	96	3	9	164	12	171	4.01e-20	82.4
Msa0534900	AT1G09740.1	32.530	166	96	3	9	164	12	171	4.01e-20	82.4
Msa0534900	AT3G62550.1	33.939	165	92	4	3	160	5	159	1.70e-18	78.2
Msa0534900	AT3G58450.1	30.636	173	94	4	9	165	34	196	2.06e-18	79.0

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Find 41 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATAGAACAATTGGTG+TGG	0.650098	4_1:-10631684	Msa0534900:CDS
AAGAAGAAATAGCTTTGACA+AGG	0.615031	4_1:+10631765	None:intergenic
AAGCTGGATTCTCTTGTTAT+GGG	0.360643	4_1:-10630674	Msa0534900:CDS
AATCAGTTATGGGCCAAAGA+TGG	0.569005	4_1:-10631542	Msa0534900:CDS
AGAAGAAATAGCTTTGACAA+GGG	0.585277	4_1:+10631766	None:intergenic
AGCATGTGAAACTAATAAAT+TGG	0.262668	4_1:+10629128	None:intergenic
AGTCACTGGGCAAGGAGCAT+TGG	0.516295	4_1:+10629390	None:intergenic
ATATTGTTACAAAAGTGTAT+TGG	0.434652	4_1:-10630739	Msa0534900:CDS
ATGAAGCTCGTAATCAGTTA+TGG	0.420885	4_1:-10631553	Msa0534900:CDS
ATGGCAAAAGATAGAACAAT+TGG	0.405644	4_1:-10631689	Msa0534900:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGGCAAAAGATAGAACAAT+TGG	-	chr4_1:10629244-10629263	Msa0534900:three_prime_UTR	30.0%
AAAAGATAGAACAATTGGTG+TGG	-	chr4_1:10629249-10629268	Msa0534900:three_prime_UTR	30.0%
CTTGAGAATTTAGCTGATAA+AGG	-	chr4_1:10629316-10629335	Msa0534900:three_prime_UTR	30.0%
TGTAAAGCTACAAAAATGAG+TGG	+	chr4_1:10630648-10630667	None:intergenic	30.0%
ATAGTGAGATCGAATAATCA+CGG	+	chr4_1:10630687-10630706	None:intergenic	30.0%
TTCATAAGTTCACAACAGAA+TGG	+	chr4_1:10630830-10630849	None:intergenic	30.0%
TTGAGATAGAATTGAGTTTG+GGG	+	chr4_1:10630959-10630978	None:intergenic	30.0%
CATTGAGATAGAATTGAGTT+TGG	+	chr4_1:10630961-10630980	None:intergenic	30.0%
GATTAAGATCTTGTAGTGAA+AGG	+	chr4_1:10631398-10631417	None:intergenic	30.0%
AAAATTTGAGTCTCATGAAC+AGG	-	chr4_1:10631486-10631505	Msa0534900:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	10629088	10631867	10629088	ID=Msa0534900;Name=Msa0534900
chr4_1	mRNA	10629088	10631867	10629088	ID=Msa0534900-mRNA-1;Parent=Msa0534900;Name=Msa0534900-mRNA-1;_AED=0.06;_eAED=0.06;_QI=156\|1\|1\|1\|1\|1\|4\|263\|166
chr4_1	exon	10629088	10629447	10629088	ID=Msa0534900-mRNA-1:exon:3407;Parent=Msa0534900-mRNA-1
chr4_1	exon	10630647	10630765	10630647	ID=Msa0534900-mRNA-1:exon:3406;Parent=Msa0534900-mRNA-1
chr4_1	exon	10630888	10630997	10630888	ID=Msa0534900-mRNA-1:exon:3405;Parent=Msa0534900-mRNA-1
chr4_1	exon	10631537	10631867	10631537	ID=Msa0534900-mRNA-1:exon:3404;Parent=Msa0534900-mRNA-1
chr4_1	five_prime_UTR	10631712	10631867	10631712	ID=Msa0534900-mRNA-1:five_prime_utr;Parent=Msa0534900-mRNA-1
chr4_1	CDS	10631537	10631711	10631537	ID=Msa0534900-mRNA-1:cds;Parent=Msa0534900-mRNA-1
chr4_1	CDS	10630888	10630997	10630888	ID=Msa0534900-mRNA-1:cds;Parent=Msa0534900-mRNA-1
chr4_1	CDS	10630647	10630765	10630647	ID=Msa0534900-mRNA-1:cds;Parent=Msa0534900-mRNA-1
chr4_1	CDS	10629351	10629447	10629351	ID=Msa0534900-mRNA-1:cds;Parent=Msa0534900-mRNA-1
chr4_1	three_prime_UTR	10629088	10629350	10629088	ID=Msa0534900-mRNA-1:three_prime_utr;Parent=Msa0534900-mRNA-1

Gene Sequence

>Msa0534900

ATGGCAAAAGATAGAACAATTGGTGTGGCTTTGGATTTCTCCAAGAGCAGCAAGAATGCTCTGAAATGGGCACTTGAGAATTTAGCTGATAAAGGTGACAACATTTACATCATTCACATCAGTCATGATTCTCTTGATGAAGCTCGTAATCAGTTATGGGCCAAAGATGGTTCTCCTCTTATTCCTTTGAAAGAGTTCAGAGAACCAGAGATAATGAAGAAGTATGGTGTTCAGATTGATATTGAGGTTCTTGATTTGCTTGACACTTTTTCAAGGCAAAAAGAGGTGAATATTGTTACAAAAGTGTATTGGGGTGATGCAAGAGAGAAACTTATGGATGCTGTTGAAGATCTGAAGCTGGATTCTCTTGTTATGGGTAGCAGGGGTCTCAGCACAATTCAAAGGATATTACTGGGAAGTGTAAGCAACTTTGTGATGACCAATGCTCCTTGCCCAGTGACTATTGTCAAGGATAATAGCAGCAGCAGCAGCAGCAAGTAA

Protein sequence

>Msa0534900

MAKDRTIGVALDFSKSSKNALKWALENLADKGDNIYIIHISHDSLDEARNQLWAKDGSPLIPLKEFREPEIMKKYGVQIDIEVLDLLDTFSRQKEVNIVTKVYWGDAREKLMDAVEDLKLDSLVMGSRGLSTIQRILLGSVSNFVMTNAPCPVTIVKDNSSSSSSK*