AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0608470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0608470 MtrunA17_Chr6g0469811 51.020 49 24 0 43 91 11 59 1.37e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATCTGTCGTTATATTTA+TGG 0.092046 4_2:-55620590 Msa0608470:three_prime_UTR
AGCAGATTAAATTTGAATTC+AGG 0.221235 4_2:+55623685 None:intergenic
CTTTAAATACCTGCATCTTC+TGG 0.293662 4_2:-55623610 Msa0608470:five_prime_UTR
CAGGTAGAACTCGGCAAAAC+TGG 0.308473 4_2:+55623946 None:intergenic
GAGAACCATCTCTATGGAAT+TGG 0.309307 4_2:+55623346 None:intergenic
AATCTGCTTTGTCTGCGTTT+TGG 0.336058 4_2:-55623670 Msa0608470:five_prime_UTR
GGTATTTAAAGACCTCAATA+AGG 0.345344 4_2:+55623622 None:intergenic
TTCAACTTTCAATTCAATAT+CGG 0.365155 4_2:-55623528 Msa0608470:CDS
ATCAATGAGAACCATCTCTA+TGG 0.370825 4_2:+55623340 None:intergenic
ACCTCAATAAGGGGCAAAAC+AGG 0.374630 4_2:+55623633 None:intergenic
AGGGATGTTTGTGGGTAGAC+AGG 0.400798 4_2:-55623795 Msa0608470:five_prime_UTR
TTTGTCCTTATGTGCTGAAT+CGG 0.403946 4_2:-55623826 Msa0608470:five_prime_UTR
CAAATTGTGTCGTGATTAGT+TGG 0.406548 4_2:-55620658 Msa0608470:three_prime_UTR
ATTGGAGTTAGATCTTCTAT+TGG 0.408232 4_2:-55623770 Msa0608470:five_prime_UTR
GCAGATTAAATTTGAATTCA+GGG 0.410557 4_2:+55623686 None:intergenic
TTGCTCCGATTCAGCACATA+AGG 0.415150 4_2:+55623821 None:intergenic
TTTGTGGGTAGACAGGATAT+TGG 0.421993 4_2:-55623788 Msa0608470:five_prime_UTR
TAAATATAACGACAGATGAT+AGG 0.422228 4_2:+55620593 None:intergenic
AGGTAAGGGTTCTGAGGTTG+TGG 0.428800 4_2:-55623400 Msa0608470:CDS
GCAACATTCATTATTCTTCG+AGG 0.431532 4_2:+55620877 None:intergenic
TCTATGGAATTGGGCTGCTC+AGG 0.436111 4_2:+55623356 None:intergenic
TGCGATTTGAGTTTGTTGTT+TGG 0.443946 4_2:-55623503 Msa0608470:CDS
CACGGTCCCACTGGAATTTG+AGG 0.444055 4_2:+55623918 None:intergenic
GATGGTTCTCATTGATGATA+AGG 0.446818 4_2:-55623333 Msa0608470:intron
TATCACCTGGTAGGGAGAAA+TGG 0.447319 4_2:-55623430 Msa0608470:CDS
AAGCGGAAGTTCAGATTAGA+AGG 0.455134 4_2:+55623886 None:intergenic
ACTGGAATTTGAGGATGAAC+AGG 0.462155 4_2:+55623927 None:intergenic
AGATGATAGGAAAGTACAAT+GGG 0.464292 4_2:+55620606 None:intergenic
AGAACCATCTCTATGGAATT+GGG 0.470154 4_2:+55623347 None:intergenic
AGAAATGGTGGCTTAAGGTA+AGG 0.470561 4_2:-55623415 Msa0608470:CDS
GTATTTAAAGACCTCAATAA+GGG 0.475314 4_2:+55623623 None:intergenic
TGCTCAGGATTCAAGAAGCT+TGG 0.480765 4_2:+55623371 None:intergenic
AGGAAAGTACAATGGGGTTA+CGG 0.498832 4_2:+55620613 None:intergenic
GGTAAGGGTTCTGAGGTTGT+GGG 0.499506 4_2:-55623399 Msa0608470:CDS
AGCAAAAGAGGGATGTTTGT+GGG 0.508721 4_2:-55623803 Msa0608470:five_prime_UTR
AGCCACCATTTCTCCCTACC+AGG 0.509535 4_2:+55623425 None:intergenic
TTGTTCGTGTGGGGTTGTAC+CGG 0.517105 4_2:-55620844 Msa0608470:CDS
TAGAAGAAACACGGTCCCAC+TGG 0.519859 4_2:+55623909 None:intergenic
GGCTTAAGGTAAGGGTTCTG+AGG 0.522560 4_2:-55623406 Msa0608470:CDS
TGTCCGCTGTATCACCTGGT+AGG 0.525200 4_2:-55623439 Msa0608470:CDS
TAGGGAGAAATGGTGGCTTA+AGG 0.528302 4_2:-55623420 Msa0608470:CDS
AACTCTCAGTCACATTCATC+CGG 0.528952 4_2:+55620825 None:intergenic
AACATTAGAAACTACAACAA+TGG 0.546775 4_2:+55620754 None:intergenic
TGCTGAATCGGAGCAAAAGA+GGG 0.552067 4_2:-55623814 Msa0608470:five_prime_UTR
CGCTTGCATCAGATCGAGGA+TGG 0.552499 4_2:-55623868 Msa0608470:five_prime_UTR
CAGATTAAATTTGAATTCAG+GGG 0.554554 4_2:+55623687 None:intergenic
TCGAGGATGGCGTGCATGAG+TGG 0.559407 4_2:-55623855 Msa0608470:five_prime_UTR
GAAATGGTGGCTTAAGGTAA+GGG 0.561830 4_2:-55623414 Msa0608470:CDS
GAGCAAAAGAGGGATGTTTG+TGG 0.563258 4_2:-55623804 Msa0608470:five_prime_UTR
GTGCTGAATCGGAGCAAAAG+AGG 0.568080 4_2:-55623815 Msa0608470:five_prime_UTR
AAGGGTTCTGAGGTTGTGGG+AGG 0.577564 4_2:-55623396 Msa0608470:CDS
TCAGTGTCCGCTGTATCACC+TGG 0.580487 4_2:-55623443 Msa0608470:CDS
CAGATGATAGGAAAGTACAA+TGG 0.580868 4_2:+55620605 None:intergenic
GTCCGCTGTATCACCTGGTA+GGG 0.583124 4_2:-55623438 Msa0608470:CDS
AATTCAAATTGAATCTGCGA+TGG 0.588162 4_2:-55623724 Msa0608470:five_prime_UTR
AGATTAAATTTGAATTCAGG+GGG 0.593133 4_2:+55623688 None:intergenic
TGACTGAGAGTTCTTTACAC+TGG 0.598840 4_2:-55620814 Msa0608470:three_prime_UTR
ATACATCAACCAGAAGATGC+AGG 0.612362 4_2:+55623601 None:intergenic
GTGCAATGAAGTATCTTCGA+AGG 0.612433 4_2:+55620784 None:intergenic
GCAGCCCAATTCCATAGAGA+TGG 0.623094 4_2:-55623351 Msa0608470:CDS
CTTCCGCTTGCATCAGATCG+AGG 0.627153 4_2:-55623872 Msa0608470:five_prime_UTR
CATCCTCGATCTGATGCAAG+CGG 0.631963 4_2:+55623869 None:intergenic
GAGGATGAACAGGTAGAACT+CGG 0.634194 4_2:+55623937 None:intergenic
TATTTAAAGACCTCAATAAG+GGG 0.658099 4_2:+55623624 None:intergenic
TGTTCATCCTCAAATTCCAG+TGG 0.662868 4_2:-55623925 Msa0608470:five_prime_UTR
CACCTGGTAGGGAGAAATGG+TGG 0.663854 4_2:-55623427 Msa0608470:CDS
GTTCATCCTCAAATTCCAGT+GGG 0.665760 4_2:-55623924 Msa0608470:five_prime_UTR
CTCCCTACCAGGTGATACAG+CGG 0.675902 4_2:+55623436 None:intergenic
ATTAGAAGGTAGAAGAAACA+CGG 0.691538 4_2:+55623900 None:intergenic
GATTAAATTTGAATTCAGGG+GGG 0.731808 4_2:+55623689 None:intergenic
GATGATAGGAAAGTACAATG+GGG 0.787127 4_2:+55620607 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTAAAAAAAATATTA+AGG + chr4_2:55622924-55622943 None:intergenic 0.0%
!!! TTTTTTTTTGATTAAAATGA+TGG - chr4_2:55622040-55622059 Msa0608470:intron 10.0%
!!! TTATTGTTATTATTTTGTTT+GGG + chr4_2:55622254-55622273 None:intergenic 10.0%
!!! ATTATTGTTATTATTTTGTT+TGG + chr4_2:55622255-55622274 None:intergenic 10.0%
!! AATATTAATATAAATGGAAA+AGG - chr4_2:55622611-55622630 Msa0608470:intron 10.0%
!! AAATATTCGTAAAAAAAATT+AGG + chr4_2:55622861-55622880 None:intergenic 10.0%
!!! TTATTCTATTAGTTTTTTTT+TGG - chr4_2:55623417-55623436 Msa0608470:CDS 10.0%
!! TAAAAAATCGTAATTAACAT+GGG + chr4_2:55621400-55621419 None:intergenic 15.0%
!! TTAAAAAATCGTAATTAACA+TGG + chr4_2:55621401-55621420 None:intergenic 15.0%
!! AAATTTGTAGAATGAAAATA+AGG + chr4_2:55621458-55621477 None:intergenic 15.0%
!!! TGAGGTTTATTTTATTAATA+GGG + chr4_2:55621646-55621665 None:intergenic 15.0%
!!! ATGAGGTTTATTTTATTAAT+AGG + chr4_2:55621647-55621666 None:intergenic 15.0%
!! ACTGACAATATTAATATAAA+TGG - chr4_2:55622605-55622624 Msa0608470:intron 15.0%
!! ATGTTATAAATCAAAGTAAA+AGG - chr4_2:55622741-55622760 Msa0608470:intron 15.0%
!! AATAACTAAAATATGTTCAA+TGG + chr4_2:55622784-55622803 None:intergenic 15.0%
!!! ATGAAATTTTTTAATGAGAA+GGG + chr4_2:55623205-55623224 None:intergenic 15.0%
!!! TAAAAAATTTCATGTTTTCA+AGG - chr4_2:55623212-55623231 Msa0608470:intron 15.0%
!! AAAAACTAATAGAATAAAAC+TGG + chr4_2:55623414-55623433 None:intergenic 15.0%
!! TTCAACTTTCAATTCAATAT+CGG - chr4_2:55621008-55621027 Msa0608470:intron 20.0%
!!! GATTTTATTAGTTTATCACT+TGG - chr4_2:55621257-55621276 Msa0608470:intron 20.0%
!! ATGAAACAAATACAAATGAA+AGG + chr4_2:55621532-55621551 None:intergenic 20.0%
!!! GAGGTTTATTTTATTAATAG+GGG + chr4_2:55621645-55621664 None:intergenic 20.0%
!!! ATCACAAGATAATTTGTTAA+GGG - chr4_2:55621824-55621843 Msa0608470:intron 20.0%
!!! TTTTTGATTAAAATGATGGA+TGG - chr4_2:55622044-55622063 Msa0608470:intron 20.0%
!! ATTATAATACTTGATAGATC+AGG + chr4_2:55622642-55622661 None:intergenic 20.0%
!!! GTTATTTTAAAGAGATCAAT+AGG - chr4_2:55622798-55622817 Msa0608470:intron 20.0%
!!! ATTAAGGTTACATATGTTTT+TGG + chr4_2:55622908-55622927 None:intergenic 20.0%
!!! ATAGATTTGATTTTGTAAAG+TGG + chr4_2:55623151-55623170 None:intergenic 20.0%
!!! CATGAAATTTTTTAATGAGA+AGG + chr4_2:55623206-55623225 None:intergenic 20.0%
!!! TATCATTAATTTTGATGAGT+TGG - chr4_2:55623294-55623313 Msa0608470:intron 20.0%
!!! ATCATTAATTTTGATGAGTT+GGG - chr4_2:55623295-55623314 Msa0608470:intron 20.0%
! AGATTAAATTTGAATTCAGG+GGG + chr4_2:55620851-55620870 None:intergenic 25.0%
! CAGATTAAATTTGAATTCAG+GGG + chr4_2:55620852-55620871 None:intergenic 25.0%
! GCAGATTAAATTTGAATTCA+GGG + chr4_2:55620853-55620872 None:intergenic 25.0%
! AGCAGATTAAATTTGAATTC+AGG + chr4_2:55620854-55620873 None:intergenic 25.0%
! TATTTAAAGACCTCAATAAG+GGG + chr4_2:55620915-55620934 None:intergenic 25.0%
! GTATTTAAAGACCTCAATAA+GGG + chr4_2:55620916-55620935 None:intergenic 25.0%
! CAAATAGCAGATAATTGAAA+AGG - chr4_2:55621347-55621366 Msa0608470:intron 25.0%
! TAAAAGCTTACATTACAATG+AGG - chr4_2:55621562-55621581 Msa0608470:intron 25.0%
! CTATGTTATCTCAAATAATG+AGG + chr4_2:55621664-55621683 None:intergenic 25.0%
!! CATTATTTGAGATAACATAG+TGG - chr4_2:55621664-55621683 Msa0608470:intron 25.0%
! ATTTAATGCCAAAAATCCAA+GGG - chr4_2:55621707-55621726 Msa0608470:intron 25.0%
!!! CATCACAAGATAATTTGTTA+AGG - chr4_2:55621823-55621842 Msa0608470:intron 25.0%
! TAAATATTATAGTGGCTACT+TGG + chr4_2:55621991-55622010 None:intergenic 25.0%
! ACATAATAAGAAATTACTGG+AGG + chr4_2:55622145-55622164 None:intergenic 25.0%
! AGCACATAATAAGAAATTAC+TGG + chr4_2:55622148-55622167 None:intergenic 25.0%
!! TATTTATAGTGTTGCTCATA+AGG - chr4_2:55622416-55622435 Msa0608470:intron 25.0%
! AACTACTTTAGCAAATTAAG+AGG + chr4_2:55622465-55622484 None:intergenic 25.0%
! GTATACTTTAGCAAATTAAG+AGG + chr4_2:55622507-55622526 None:intergenic 25.0%
!!! GACATTTTTTTGTTTATTGC+AGG + chr4_2:55622555-55622574 None:intergenic 25.0%
! GATATGCATAAGTTATTTGT+AGG - chr4_2:55622820-55622839 Msa0608470:intron 25.0%
! TTTATCTCCATCAAATCTAA+CGG + chr4_2:55622952-55622971 None:intergenic 25.0%
!!! AAATTTCATGTTTTCAAGGA+TGG - chr4_2:55623216-55623235 Msa0608470:intron 25.0%
!!! AATTTCATGTTTTCAAGGAT+GGG - chr4_2:55623217-55623236 Msa0608470:intron 25.0%
! TAGAACAATACCTTAACAAA+AGG + chr4_2:55623386-55623405 None:intergenic 25.0%
! ATTACGACATTCATGAAAAT+TGG + chr4_2:55623535-55623554 None:intergenic 25.0%
! TACTCTATTTGATATTGTTC+AGG - chr4_2:55623622-55623641 Msa0608470:five_prime_UTR 25.0%
! AACATTAGAAACTACAACAA+TGG + chr4_2:55623785-55623804 None:intergenic 25.0%
! TAAATATAACGACAGATGAT+AGG + chr4_2:55623946-55623965 None:intergenic 25.0%
! ATCATCTGTCGTTATATTTA+TGG - chr4_2:55623946-55623965 Msa0608470:five_prime_UTR 25.0%
ATTAGAAGGTAGAAGAAACA+CGG + chr4_2:55620639-55620658 None:intergenic 30.0%
ATTGGAGTTAGATCTTCTAT+TGG - chr4_2:55620766-55620785 Msa0608470:three_prime_UTR 30.0%
AATTCAAATTGAATCTGCGA+TGG - chr4_2:55620812-55620831 Msa0608470:three_prime_UTR 30.0%
GATTAAATTTGAATTCAGGG+GGG + chr4_2:55620850-55620869 None:intergenic 30.0%
GGTATTTAAAGACCTCAATA+AGG + chr4_2:55620917-55620936 None:intergenic 30.0%
! ATTTGCAATTTTCATCTGTG+AGG - chr4_2:55620969-55620988 Msa0608470:intron 30.0%
!! ATCACTTGGTTCTATGAATT+TGG - chr4_2:55621271-55621290 Msa0608470:intron 30.0%
!! TCACTTGGTTCTATGAATTT+GGG - chr4_2:55621272-55621291 Msa0608470:intron 30.0%
GATAATTGAAAAGGAAGAGT+AGG - chr4_2:55621356-55621375 Msa0608470:intron 30.0%
AAGCTTATCAAAGTTCAAGA+GGG + chr4_2:55621497-55621516 None:intergenic 30.0%
! TTGTAATGTAAGCTTTTAGC+AGG + chr4_2:55621560-55621579 None:intergenic 30.0%
TTAATAGGGGATTTAGTCTA+GGG + chr4_2:55621632-55621651 None:intergenic 30.0%
ATTAATAGGGGATTTAGTCT+AGG + chr4_2:55621633-55621652 None:intergenic 30.0%
!!! CTTGGATTTTTGGCATTAAA+TGG + chr4_2:55621708-55621727 None:intergenic 30.0%
CATTTAATGCCAAAAATCCA+AGG - chr4_2:55621706-55621725 Msa0608470:intron 30.0%
! TCTCTCACATTAGATTTTCT+GGG - chr4_2:55621747-55621766 Msa0608470:intron 30.0%
TATTAATGATGATAGCCATC+TGG - chr4_2:55622069-55622088 Msa0608470:intron 30.0%
TACTTTAGCAAATTAAGAGG+TGG + chr4_2:55622462-55622481 None:intergenic 30.0%
TACTTTAGCAAATTAAGAGG+TGG + chr4_2:55622462-55622481 None:intergenic 30.0%
AAAGTATACGCTATCAAACA+TGG - chr4_2:55622518-55622537 Msa0608470:intron 30.0%
TTATTAACCCTTAGAGTTGA+TGG - chr4_2:55622879-55622898 Msa0608470:intron 30.0%
! TATTAACCCTTAGAGTTGAT+GGG - chr4_2:55622880-55622899 Msa0608470:intron 30.0%
ATATTACCCGTTAGATTTGA+TGG - chr4_2:55622942-55622961 Msa0608470:intron 30.0%
TTATCTCCATCAAATCTAAC+GGG + chr4_2:55622951-55622970 None:intergenic 30.0%
! AGATGATAGGAAAGTACAAT+GGG + chr4_2:55623933-55623952 None:intergenic 30.0%
TTTGTCCTTATGTGCTGAAT+CGG - chr4_2:55620710-55620729 Msa0608470:three_prime_UTR 35.0%
CTTTAAATACCTGCATCTTC+TGG - chr4_2:55620926-55620945 Msa0608470:intron 35.0%
!! TGCGATTTGAGTTTGTTGTT+TGG - chr4_2:55621033-55621052 Msa0608470:intron 35.0%
AGAACCATCTCTATGGAATT+GGG + chr4_2:55621192-55621211 None:intergenic 35.0%
ATCAATGAGAACCATCTCTA+TGG + chr4_2:55621199-55621218 None:intergenic 35.0%
! GATGGTTCTCATTGATGATA+AGG - chr4_2:55621203-55621222 Msa0608470:intron 35.0%
CAAGCTTATCAAAGTTCAAG+AGG + chr4_2:55621498-55621517 None:intergenic 35.0%
AGCTTACATTACAATGAGGA+TGG - chr4_2:55621566-55621585 Msa0608470:intron 35.0%
GCTTACATTACAATGAGGAT+GGG - chr4_2:55621567-55621586 Msa0608470:intron 35.0%
! CTCTCTCACATTAGATTTTC+TGG - chr4_2:55621746-55621765 Msa0608470:intron 35.0%
TCAGAGAATAGCTAAAGCTT+AGG - chr4_2:55621859-55621878 Msa0608470:intron 35.0%
CAGAGAATAGCTAAAGCTTA+GGG - chr4_2:55621860-55621879 Msa0608470:intron 35.0%
AAAGCTTAGGGAACAAACTA+TGG - chr4_2:55621872-55621891 Msa0608470:intron 35.0%
AAGCTTAGGGAACAAACTAT+GGG - chr4_2:55621873-55621892 Msa0608470:intron 35.0%
GTATCATTATGCTGTTGTAG+TGG - chr4_2:55622195-55622214 Msa0608470:intron 35.0%
CTAAGCTTCCAACCATTATT+AGG - chr4_2:55622295-55622314 Msa0608470:intron 35.0%
TTCAATGTTGATGTCACTGT+TGG + chr4_2:55622681-55622700 None:intergenic 35.0%
!! ATTAACCCTTAGAGTTGATG+GGG - chr4_2:55622881-55622900 Msa0608470:intron 35.0%
TGAAAGATAAGAAGGAAGGT+GGG + chr4_2:55623180-55623199 None:intergenic 35.0%
TAGCAGAATAAACACATGCA+AGG + chr4_2:55623266-55623285 None:intergenic 35.0%
!! TAATTTTGATGAGTTGGGAG+CGG - chr4_2:55623300-55623319 Msa0608470:intron 35.0%
TGTAGTGTTCTTCCATTGTT+AGG - chr4_2:55623343-55623362 Msa0608470:CDS 35.0%
TTAACAAAAGGTAATCGTCC+AGG + chr4_2:55623374-55623393 None:intergenic 35.0%
! TGGACGATTACCTTTTGTTA+AGG - chr4_2:55623373-55623392 Msa0608470:CDS 35.0%
GCAACATTCATTATTCTTCG+AGG + chr4_2:55623662-55623681 None:intergenic 35.0%
!! AGTTGACTTTTTGTTCGTGT+GGG - chr4_2:55623682-55623701 Msa0608470:five_prime_UTR 35.0%
CAAATTGTGTCGTGATTAGT+TGG - chr4_2:55623878-55623897 Msa0608470:five_prime_UTR 35.0%
! GATGATAGGAAAGTACAATG+GGG + chr4_2:55623932-55623951 None:intergenic 35.0%
! CAGATGATAGGAAAGTACAA+TGG + chr4_2:55623934-55623953 None:intergenic 35.0%
ACTGGAATTTGAGGATGAAC+AGG + chr4_2:55620612-55620631 None:intergenic 40.0%
TGTTCATCCTCAAATTCCAG+TGG - chr4_2:55620611-55620630 Msa0608470:three_prime_UTR 40.0%
GTTCATCCTCAAATTCCAGT+GGG - chr4_2:55620612-55620631 Msa0608470:three_prime_UTR 40.0%
AAGCGGAAGTTCAGATTAGA+AGG + chr4_2:55620653-55620672 None:intergenic 40.0%
AGCAAAAGAGGGATGTTTGT+GGG - chr4_2:55620733-55620752 Msa0608470:three_prime_UTR 40.0%
TTTGTGGGTAGACAGGATAT+TGG - chr4_2:55620748-55620767 Msa0608470:three_prime_UTR 40.0%
! AATCTGCTTTGTCTGCGTTT+TGG - chr4_2:55620866-55620885 Msa0608470:CDS 40.0%
ATACATCAACCAGAAGATGC+AGG + chr4_2:55620938-55620957 None:intergenic 40.0%
AGAAATGGTGGCTTAAGGTA+AGG - chr4_2:55621121-55621140 Msa0608470:intron 40.0%
GAAATGGTGGCTTAAGGTAA+GGG - chr4_2:55621122-55621141 Msa0608470:intron 40.0%
GAGAACCATCTCTATGGAAT+TGG + chr4_2:55621193-55621212 None:intergenic 40.0%
GTAGGATATGTCATTGCACT+GGG + chr4_2:55621915-55621934 None:intergenic 40.0%
AGTAGGATATGTCATTGCAC+TGG + chr4_2:55621916-55621935 None:intergenic 40.0%
TCTGCAACAAAGCAATCAGT+AGG + chr4_2:55621933-55621952 None:intergenic 40.0%
CATAAGCAACAACTACCAGA+TGG + chr4_2:55622087-55622106 None:intergenic 40.0%
! CATCTGGTAGTTGTTGCTTA+TGG - chr4_2:55622085-55622104 Msa0608470:intron 40.0%
TGAGCTTCCAATTCCAACAT+GGG + chr4_2:55622230-55622249 None:intergenic 40.0%
CTTCCAACCATTATTAGGCT+TGG - chr4_2:55622300-55622319 Msa0608470:intron 40.0%
GTTCCAAGCCTAATAATGGT+TGG + chr4_2:55622306-55622325 None:intergenic 40.0%
!! CGAGTAGAGTATTGTGAAAC+TGG + chr4_2:55622579-55622598 None:intergenic 40.0%
TACTGCTCGTTACACTGAAA+TGG + chr4_2:55623010-55623029 None:intergenic 40.0%
AGCAGTAATTAGACCGTCAA+CGG - chr4_2:55623023-55623042 Msa0608470:intron 40.0%
! CCATTGTTTGTTACCGTTGA+CGG + chr4_2:55623039-55623058 None:intergenic 40.0%
CGTCAACGGTAACAAACAAT+GGG - chr4_2:55623037-55623056 Msa0608470:intron 40.0%
GGCTATGAACAGTGTTTCAA+CGG - chr4_2:55623109-55623128 Msa0608470:intron 40.0%
GTGAAAGATAAGAAGGAAGG+TGG + chr4_2:55623181-55623200 None:intergenic 40.0%
GGTGTGAAAGATAAGAAGGA+AGG + chr4_2:55623184-55623203 None:intergenic 40.0%
! GAAGGGTGTGAAAGATAAGA+AGG + chr4_2:55623188-55623207 None:intergenic 40.0%
AGCGGTCTTTGTAGTGTTAA+TGG - chr4_2:55623318-55623337 Msa0608470:intron 40.0%
!! CAGTTGACTTTTTGTTCGTG+TGG - chr4_2:55623681-55623700 Msa0608470:five_prime_UTR 40.0%
!! GTTGACTTTTTGTTCGTGTG+GGG - chr4_2:55623683-55623702 Msa0608470:five_prime_UTR 40.0%
AACTCTCAGTCACATTCATC+CGG + chr4_2:55623714-55623733 None:intergenic 40.0%
TGACTGAGAGTTCTTTACAC+TGG - chr4_2:55623722-55623741 Msa0608470:five_prime_UTR 40.0%
! GTGCAATGAAGTATCTTCGA+AGG + chr4_2:55623755-55623774 None:intergenic 40.0%
! AGGAAAGTACAATGGGGTTA+CGG + chr4_2:55623926-55623945 None:intergenic 40.0%
GAGGATGAACAGGTAGAACT+CGG + chr4_2:55620602-55620621 None:intergenic 45.0%
TTGCTCCGATTCAGCACATA+AGG + chr4_2:55620718-55620737 None:intergenic 45.0%
TGCTGAATCGGAGCAAAAGA+GGG - chr4_2:55620722-55620741 Msa0608470:three_prime_UTR 45.0%
GAGCAAAAGAGGGATGTTTG+TGG - chr4_2:55620732-55620751 Msa0608470:three_prime_UTR 45.0%
! TCCTGTTTTGCCCCTTATTG+AGG - chr4_2:55620902-55620921 Msa0608470:intron 45.0%
ACCTCAATAAGGGGCAAAAC+AGG + chr4_2:55620906-55620925 None:intergenic 45.0%
TATCACCTGGTAGGGAGAAA+TGG - chr4_2:55621106-55621125 Msa0608470:intron 45.0%
TAGGGAGAAATGGTGGCTTA+AGG - chr4_2:55621116-55621135 Msa0608470:intron 45.0%
TGCTCAGGATTCAAGAAGCT+TGG + chr4_2:55621168-55621187 None:intergenic 45.0%
!! CAATGAGGATGGGTTGAGTT+TGG - chr4_2:55621577-55621596 Msa0608470:intron 45.0%
TGCCAAAAATCCAAGGGATC+AGG - chr4_2:55621713-55621732 Msa0608470:intron 45.0%
! TGCCTGATCCCTTGGATTTT+TGG + chr4_2:55621718-55621737 None:intergenic 45.0%
ATCCAAGGGATCAGGCAATT+TGG - chr4_2:55621721-55621740 Msa0608470:intron 45.0%
AACCAAATTGCCTGATCCCT+TGG + chr4_2:55621726-55621745 None:intergenic 45.0%
CTGGAGGAGATTAACAACTC+TGG + chr4_2:55622129-55622148 None:intergenic 45.0%
AATTCCAACATGGGATGGCT+CGG + chr4_2:55622221-55622240 None:intergenic 45.0%
CTTCCAATTCCAACATGGGA+TGG + chr4_2:55622226-55622245 None:intergenic 45.0%
CTGAGCTTCCAATTCCAACA+TGG + chr4_2:55622231-55622250 None:intergenic 45.0%
CCTCGTTCCAAGCCTAATAA+TGG + chr4_2:55622310-55622329 None:intergenic 45.0%
CCATTATTAGGCTTGGAACG+AGG - chr4_2:55622307-55622326 Msa0608470:intron 45.0%
TTGGTCCCCATCAACTCTAA+GGG + chr4_2:55622889-55622908 None:intergenic 45.0%
TTTGGTCCCCATCAACTCTA+AGG + chr4_2:55622890-55622909 None:intergenic 45.0%
TGCTCGTTACACTGAAATGG+TGG + chr4_2:55623007-55623026 None:intergenic 45.0%
CCGTCAACGGTAACAAACAA+TGG - chr4_2:55623036-55623055 Msa0608470:intron 45.0%
!! AATTAATTTAAATATTATAG+TGG + chr4_2:55621999-55622018 None:intergenic 5.0%
!! AATTTAAATAATTAAACAAA+CGG - chr4_2:55623088-55623107 Msa0608470:intron 5.0%
!!! AAATTTTATGTATTAAAAAA+CGG - chr4_2:55623497-55623516 Msa0608470:CDS 5.0%
CAGGTAGAACTCGGCAAAAC+TGG + chr4_2:55620593-55620612 None:intergenic 50.0%
TAGAAGAAACACGGTCCCAC+TGG + chr4_2:55620630-55620649 None:intergenic 50.0%
CATCCTCGATCTGATGCAAG+CGG + chr4_2:55620670-55620689 None:intergenic 50.0%
GTGCTGAATCGGAGCAAAAG+AGG - chr4_2:55620721-55620740 Msa0608470:three_prime_UTR 50.0%
AGGGATGTTTGTGGGTAGAC+AGG - chr4_2:55620741-55620760 Msa0608470:three_prime_UTR 50.0%
GGCTTAAGGTAAGGGTTCTG+AGG - chr4_2:55621130-55621149 Msa0608470:intron 50.0%
!! AGGTAAGGGTTCTGAGGTTG+TGG - chr4_2:55621136-55621155 Msa0608470:intron 50.0%
!! GGTAAGGGTTCTGAGGTTGT+GGG - chr4_2:55621137-55621156 Msa0608470:intron 50.0%
TCTATGGAATTGGGCTGCTC+AGG + chr4_2:55621183-55621202 None:intergenic 50.0%
GCAGCCCAATTCCATAGAGA+TGG - chr4_2:55621185-55621204 Msa0608470:intron 50.0%
ATTGCACTGGGACTGCTACA+CGG + chr4_2:55621903-55621922 None:intergenic 50.0%
TTATGGCCTTCTCCAGTGCT+AGG - chr4_2:55622102-55622121 Msa0608470:intron 50.0%
AACAACTCTGGTCCTAGCAC+TGG + chr4_2:55622117-55622136 None:intergenic 50.0%
GAGCCATCCCATGTTGGAAT+TGG - chr4_2:55622220-55622239 Msa0608470:intron 50.0%
CATGCAAGGTGAAGCAGAGA+AGG + chr4_2:55623252-55623271 None:intergenic 50.0%
TTCCATTGTTAGGCTTCGCC+TGG - chr4_2:55623353-55623372 Msa0608470:CDS 50.0%
TTGTTCGTGTGGGGTTGTAC+CGG - chr4_2:55623692-55623711 Msa0608470:five_prime_UTR 50.0%
CACGGTCCCACTGGAATTTG+AGG + chr4_2:55620621-55620640 None:intergenic 55.0%
CTTCCGCTTGCATCAGATCG+AGG - chr4_2:55620664-55620683 Msa0608470:three_prime_UTR 55.0%
CGCTTGCATCAGATCGAGGA+TGG - chr4_2:55620668-55620687 Msa0608470:three_prime_UTR 55.0%
TCAGTGTCCGCTGTATCACC+TGG - chr4_2:55621093-55621112 Msa0608470:intron 55.0%
TGTCCGCTGTATCACCTGGT+AGG - chr4_2:55621097-55621116 Msa0608470:intron 55.0%
GTCCGCTGTATCACCTGGTA+GGG - chr4_2:55621098-55621117 Msa0608470:intron 55.0%
CTCCCTACCAGGTGATACAG+CGG + chr4_2:55621103-55621122 None:intergenic 55.0%
CACCTGGTAGGGAGAAATGG+TGG - chr4_2:55621109-55621128 Msa0608470:intron 55.0%
AGCCACCATTTCTCCCTACC+AGG + chr4_2:55621114-55621133 None:intergenic 55.0%
!! AAGGGTTCTGAGGTTGTGGG+AGG - chr4_2:55621140-55621159 Msa0608470:intron 55.0%
! TCTGGTCCTAGCACTGGAGA+AGG + chr4_2:55622111-55622130 None:intergenic 55.0%
GTCCAGGCGAAGCCTAACAA+TGG + chr4_2:55623358-55623377 None:intergenic 55.0%
TCGAGGATGGCGTGCATGAG+TGG - chr4_2:55620681-55620700 Msa0608470:three_prime_UTR 60.0%
GTGGCCGAGCCATCCCATGT+TGG - chr4_2:55622214-55622233 Msa0608470:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 55620561 55623997 55620561 ID=Msa0608470;Name=Msa0608470
chr4_2 mRNA 55620561 55623997 55620561 ID=Msa0608470-mRNA-1;Parent=Msa0608470;Name=Msa0608470-mRNA-1;_AED=0.17;_eAED=0.17;_QI=391|1|1|1|0|0.5|2|279|115
chr4_2 exon 55620561 55620914 55620561 ID=Msa0608470-mRNA-1:exon:14226;Parent=Msa0608470-mRNA-1
chr4_2 exon 55623334 55623997 55623334 ID=Msa0608470-mRNA-1:exon:14225;Parent=Msa0608470-mRNA-1
chr4_2 five_prime_UTR 55623607 55623997 55623607 ID=Msa0608470-mRNA-1:five_prime_utr;Parent=Msa0608470-mRNA-1
chr4_2 CDS 55623334 55623606 55623334 ID=Msa0608470-mRNA-1:cds;Parent=Msa0608470-mRNA-1
chr4_2 CDS 55620840 55620914 55620840 ID=Msa0608470-mRNA-1:cds;Parent=Msa0608470-mRNA-1
chr4_2 three_prime_UTR 55620561 55620839 55620561 ID=Msa0608470-mRNA-1:three_prime_utr;Parent=Msa0608470-mRNA-1
Gene Sequence

>Msa0608470

ATGTATTCACTTTCACAATTTGCAATTTTCATCTGTGAGGAGAGATTGTTGTTACTTTCAACTTTCAATTCAATATCGGTCTGCGATTTGAGTTTGTTGTTTGGCTTGATGTCGAAGTCTGTGATTGATAACTCATTTGACTCAGTGTCCGCTGTATCACCTGGTAGGGAGAAATGGTGGCTTAAGGTAAGGGTTCTGAGGTTGTGGGAGGTGCCAAGCTTCTTGAATCCTGAGCAGCCCAATTCCATAGAGATGGTTCTCATTGATGATAAGGTAAAAATGCAGTATCCTCGAAGAATAATGAATGTTGCAGTTGACTTTTTGTTCGTGTGGGGTTGTACCGGATGA
Protein sequence

>Msa0608470

MYSLSQFAIFICEERLLLLSTFNSISVCDLSLLFGLMSKSVIDNSFDSVSAVSPGREKWWLKVRVLRLWEVPSFLNPEQPNSIEMVLIDDKVKMQYPRRIMNVAVDFLFVWGCTG*