AlfalfaGEDB | Msa0610120

M. sativa cultivar zhongmu-4 / Msa0610120

KEGG: -
GO: -
PFAM: PF05875.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0610120	XP_024628984.1	91.045	268	8	1	1	268	1	252	3.93e-177	499

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0610120	A0A072U4J5	91.045	268	8	1	1	268	1	252	1.88e-177	499

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa0610120	0.817739	2.819900e-52	-8.615850e-47
Msa0157290	Msa0610120	0.817524	3.153896e-52	-8.615850e-47
Msa0264150	Msa0610120	0.806537	7.841008e-50	-8.615850e-47
Msa0436050	Msa0610120	0.818469	1.928263e-52	-8.615850e-47
Msa0484780	Msa0610120	0.801967	7.011630e-49	-8.615850e-47
Msa0610120	Msa0880140	0.812832	3.476657e-51	-8.615850e-47
Msa0610120	Msa1082500	0.805494	1.299550e-49	-8.615850e-47
Msa0610120	Msa1114250	0.800425	1.449772e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0610120	MtrunA17_Chr8g0381391	92.021	188	13	1	1	186	1	188	1.40e-124	354
Msa0610120	MtrunA17_Chr2g0277671	63.498	263	80	1	1	263	1	247	5.50e-114	331
Msa0610120	MtrunA17_Chr3g0135701	38.367	245	135	1	19	263	32	260	1.92e-59	191
Msa0610120	MtrunA17_Chr1g0151401	36.800	250	140	2	24	271	45	278	4.64e-51	170
Msa0610120	MtrunA17_Chr8g0381381	93.243	74	5	0	195	268	1	74	5.69e-44	145

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0610120	AT1G71190.1	59.160	262	90	2	1	261	1	246	3.20e-103	303
Msa0610120	AT1G71190.2	48.659	261	78	2	1	261	1	205	5.43e-81	246
Msa0610120	AT5G11870.1	39.922	258	135	3	10	263	17	258	2.00e-67	212
Msa0610120	AT5G11870.2	39.922	258	135	3	10	263	17	258	3.31e-67	211

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Find 102 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTCCCAAGGTGAGCTA+TGG	0.477754	4_2:+57808100	Msa0610120:intron
AAGAAGAGAAAATGGGTCAC+TGG	0.500287	4_2:-57807240	None:intergenic
AAGAAGGCACTGCACAAGCA+TGG	0.560374	4_2:+57807293	Msa0610120:CDS
AAGGTGCTTAATTAGTCATA+GGG	0.551925	4_2:+57810772	Msa0610120:three_prime_UTR
AAGGTTGCGCTACTAAGGGT+AGG	0.606006	4_2:+57810641	Msa0610120:three_prime_UTR
AAGTTCCCAAGGTGAGCTAT+GGG	0.503035	4_2:+57808101	Msa0610120:intron
AATTTACCTTTCATGGTTAT+TGG	0.214237	4_2:+57807547	Msa0610120:CDS
ACACTGAAGCATTTATCTGC+TGG	0.516057	4_2:+57809735	Msa0610120:CDS
ACAGAAAGGTTGCGCTACTA+AGG	0.403949	4_2:+57810636	Msa0610120:three_prime_UTR
ACAGTTTGGACCAGAAGATA+TGG	0.451186	4_2:-57809468	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGAATGTAAGAAGAGAAAA+TGG	-	chr4_2:57807251-57807270	None:intergenic	30.0%
TAAGCAACAACAATGAAAGA+AGG	+	chr4_2:57807277-57807296	Msa0610120:exon	30.0%
TGAATCATTTCAGACCAATT+TGG	+	chr4_2:57807438-57807457	Msa0610120:intron	30.0%
TTGTGGGTCTTTCATTTAAA+GGG	+	chr4_2:57807463-57807482	Msa0610120:intron	30.0%
GATATCACATTAAGTGCATT+GGG	-	chr4_2:57807526-57807545	None:intergenic	30.0%
TGATATCACATTAAGTGCAT+TGG	-	chr4_2:57807527-57807546	None:intergenic	30.0%
ATAAGACCAATAACCATGAA+AGG	-	chr4_2:57807556-57807575	None:intergenic	30.0%
CCAAACTATAATTCCTAAGA+TGG	-	chr4_2:57807627-57807646	None:intergenic	30.0%
CCATCTTAGGAATTATAGTT+TGG	+	chr4_2:57807624-57807643	Msa0610120:intron	30.0%
AAAATTGAGTGAATAACCGA+GGG	-	chr4_2:57808024-57808043	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	57807060	57810987	57807060	ID=Msa0610120;Name=Msa0610120
chr4_2	mRNA	57807060	57810987	57807060	ID=Msa0610120-mRNA-1;Parent=Msa0610120;Name=Msa0610120-mRNA-1;_AED=0.23;_eAED=0.23;_QI=229\|0.66\|0.71\|1\|1\|1\|7\|396\|311
chr4_2	exon	57807060	57807415	57807060	ID=Msa0610120-mRNA-1:exon:15041;Parent=Msa0610120-mRNA-1
chr4_2	exon	57807518	57807617	57807518	ID=Msa0610120-mRNA-1:exon:15042;Parent=Msa0610120-mRNA-1
chr4_2	exon	57808104	57808224	57808104	ID=Msa0610120-mRNA-1:exon:15043;Parent=Msa0610120-mRNA-1
chr4_2	exon	57808329	57808498	57808329	ID=Msa0610120-mRNA-1:exon:15044;Parent=Msa0610120-mRNA-1
chr4_2	exon	57809449	57809570	57809449	ID=Msa0610120-mRNA-1:exon:15045;Parent=Msa0610120-mRNA-1
chr4_2	exon	57809655	57809873	57809655	ID=Msa0610120-mRNA-1:exon:15046;Parent=Msa0610120-mRNA-1
chr4_2	exon	57810515	57810987	57810515	ID=Msa0610120-mRNA-1:exon:15047;Parent=Msa0610120-mRNA-1
chr4_2	five_prime_UTR	57807060	57807288	57807060	ID=Msa0610120-mRNA-1:five_prime_utr;Parent=Msa0610120-mRNA-1
chr4_2	CDS	57807289	57807415	57807289	ID=Msa0610120-mRNA-1:cds;Parent=Msa0610120-mRNA-1
chr4_2	CDS	57807518	57807617	57807518	ID=Msa0610120-mRNA-1:cds;Parent=Msa0610120-mRNA-1
chr4_2	CDS	57808104	57808224	57808104	ID=Msa0610120-mRNA-1:cds;Parent=Msa0610120-mRNA-1
chr4_2	CDS	57808329	57808498	57808329	ID=Msa0610120-mRNA-1:cds;Parent=Msa0610120-mRNA-1
chr4_2	CDS	57809449	57809570	57809449	ID=Msa0610120-mRNA-1:cds;Parent=Msa0610120-mRNA-1
chr4_2	CDS	57809655	57809873	57809655	ID=Msa0610120-mRNA-1:cds;Parent=Msa0610120-mRNA-1
chr4_2	CDS	57810515	57810591	57810515	ID=Msa0610120-mRNA-1:cds;Parent=Msa0610120-mRNA-1
chr4_2	three_prime_UTR	57810592	57810987	57810592	ID=Msa0610120-mRNA-1:three_prime_utr;Parent=Msa0610120-mRNA-1

Gene Sequence

>Msa0610120

ATGAAAGAAGGCACTGCACAAGCATGGGTAGTTGCGGTAGCTTCATTCTATCTATTGATGAAGCTAACCCCATCCATTCCTCAACCTCAGATGTACCATGATTTCGCTGATAAGCGTGAATTCTTTGGTATTCCCAATGCACTTAATGTGATATCAAATTTACCTTTCATGGTTATTGGTCTTATTGGGCTTATGCTTTGTCATCGTAGCAACTATTTTAACTTAAGTTCCCAAGGTGAGCTATGGGGTTGGACATGTTTTTATGTGGCTGTGACTTCAGTAGGTTTTGGGTCTGCATATTATCATCTCGGGCCAAATGATAATGGCCTTGTTTGCGACAGGTTGCCGATGACTGTTGCTTTTGCTTCACTGGTGGCAATTCTCATCATTGAAAGGGTTGATGCAAAGAAGGGAACGATTTCAATTTTTCCTCTAATAATGGCTGCTATGATAAGTAGTGTGTATTGGAGGCAAGCTAATTCTGTCTCTTTTACTTGGAAGAGTTATTTTTCTTACTCGTTCTTTGGTGACATCCGTCCATATCTTCTGGTCCAAACTGTATCATGCATTGCTGTACCTCTCATGGCATTTTTGTTGCCTCCAATGTACACACATTCAACGTATTGGCTATGGGCTGCAGGATTTTACCCTCTAGCTATGATGCAAGAGACTGCCGATAGATTGATTTATGCAGTGACTTTTCATACTGTCAGTGGTCACACACTGAAGCATTTATCTGCTGGAATGGTTCCTCTCATCTTAACAATAATGCTTGCAAAGAGGAGGCTTTTGCATGTAAGCAACATACAATCCTTCAAGTTATTGAATTTGCTACTGTACAACTTTTTATTATGTGTTGGCAAAATCTGCTTGATGCATACTGCATTTGCTGTACTAAGGGCAAAGAAGGTGACTGATCTCAATGTGCAGCTATAG

Protein sequence

>Msa0610120

MKEGTAQAWVVAVASFYLLMKLTPSIPQPQMYHDFADKREFFGIPNALNVISNLPFMVIGLIGLMLCHRSNYFNLSSQGELWGWTCFYVAVTSVGFGSAYYHLGPNDNGLVCDRLPMTVAFASLVAILIIERVDAKKGTISIFPLIMAAMISSVYWRQANSVSFTWKSYFSYSFFGDIRPYLLVQTVSCIAVPLMAFLLPPMYTHSTYWLWAAGFYPLAMMQETADRLIYAVTFHTVSGHTLKHLSAGMVPLILTIMLAKRRLLHVSNIQSFKLLNLLLYNFLLCVGKICLMHTAFAVLRAKKVTDLNVQL*