AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0610760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610760 MtrunA17_Chr4g0043851 91.960 398 31 1 1 397 1 398 0.0 741
Msa0610760 MtrunA17_Chr2g0285901 76.106 339 75 2 12 350 9 341 0.0 538
Msa0610760 MtrunA17_Chr1g0189111 56.231 329 143 1 23 351 22 349 6.05e-136 395
Msa0610760 MtrunA17_Chr4g0026321 52.632 323 153 0 31 353 28 350 5.67e-126 369
Msa0610760 MtrunA17_Chr2g0321511 49.563 343 170 2 9 350 5 345 3.07e-123 362
Msa0610760 MtrunA17_Chr4g0003281 48.368 337 173 1 30 365 38 374 4.14e-117 346
Msa0610760 MtrunA17_Chr4g0064021 45.846 325 175 1 30 353 24 348 1.11e-102 309
Msa0610760 MtrunA17_Chr5g0439251 49.231 325 162 3 29 351 22 345 1.06e-99 305
Msa0610760 MtrunA17_Chr3g0144531 43.407 364 199 3 1 362 1 359 4.91e-99 302
Msa0610760 MtrunA17_Chr4g0034901 44.444 351 193 2 14 362 10 360 4.40e-97 297
Msa0610760 MtrunA17_Chr1g0155001 46.584 322 170 2 32 351 24 345 4.94e-97 296
Msa0610760 MtrunA17_Chr1g0177171 45.706 326 174 3 28 351 34 358 3.89e-95 293
Msa0610760 MtrunA17_Chr3g0119541 45.294 340 178 4 30 362 24 362 8.08e-95 288
Msa0610760 MtrunA17_Chr1g0146831 45.846 325 170 3 25 347 24 344 2.03e-93 285
Msa0610760 MtrunA17_Chr8g0389741 42.609 345 189 3 33 376 29 365 1.93e-89 279
Msa0610760 MtrunA17_Chr6g0487261 45.312 320 173 2 33 351 28 346 2.20e-87 274
Msa0610760 MtrunA17_Chr7g0223961 44.753 324 171 5 29 350 20 337 1.58e-86 270
Msa0610760 MtrunA17_Chr7g0234821 42.462 325 181 5 33 352 27 350 4.45e-86 269
Msa0610760 MtrunA17_Chr8g0339191 38.655 357 210 6 1 352 1 353 4.74e-82 259
Msa0610760 MtrunA17_Chr4g0046311 39.394 330 192 5 30 352 21 349 1.47e-78 250
Msa0610760 MtrunA17_Chr4g0043511 41.667 348 194 7 7 350 37 379 1.21e-76 247
Msa0610760 MtrunA17_Chr3g0121271 37.576 330 200 4 14 338 7 335 2.71e-76 245
Msa0610760 MtrunA17_Chr4g0075031 39.144 327 194 3 32 354 45 370 2.72e-75 243
Msa0610760 MtrunA17_Chr8g0378621 37.383 321 196 3 30 346 24 343 5.89e-74 239
Msa0610760 MtrunA17_Chr1g0161011 38.506 348 208 4 8 351 1 346 1.58e-73 238
Msa0610760 MtrunA17_Chr4g0039011 40.123 324 182 6 30 353 40 351 2.41e-73 233
Msa0610760 MtrunA17_Chr5g0434591 38.938 339 194 5 8 339 1 333 1.43e-72 235
Msa0610760 MtrunA17_Chr3g0110371 36.826 334 206 3 10 339 2 334 2.23e-69 226
Msa0610760 MtrunA17_Chr4g0038981 38.053 339 195 9 8 346 1 324 1.28e-66 214
Msa0610760 MtrunA17_Chr4g0038971 37.537 341 198 10 8 348 1 326 1.47e-66 214
Msa0610760 MtrunA17_Chr4g0038961 37.719 342 196 10 8 348 1 326 4.40e-66 213
Msa0610760 MtrunA17_Chr4g0038941 39.009 323 184 6 26 348 46 355 2.32e-65 212
Msa0610760 MtrunA17_Chr2g0294601 37.429 350 209 7 5 352 32 373 2.05e-63 208
Msa0610760 MtrunA17_Chr4g0039071 38.415 328 192 7 24 349 17 336 2.65e-63 207
Msa0610760 MtrunA17_Chr7g0250301 34.365 323 202 6 25 346 1 314 1.34e-62 204
Msa0610760 MtrunA17_Chr2g0294591 35.241 332 203 8 30 358 28 350 3.09e-58 194
Msa0610760 MtrunA17_Chr1g0174261 38.754 289 167 6 62 348 2 282 1.90e-57 191
Msa0610760 MtrunA17_Chr4g0039021 37.676 284 167 5 58 340 7 281 2.02e-55 184
Msa0610760 MtrunA17_Chr1g0148391 33.989 356 214 13 6 352 4 347 1.97e-52 181
Msa0610760 MtrunA17_Chr7g0250321 33.219 292 184 6 7 297 5 286 8.02e-51 172
Msa0610760 MtrunA17_Chr3g0138741 31.831 355 226 11 5 352 7 352 7.06e-49 172
Msa0610760 MtrunA17_Chr4g0062771 32.231 363 222 14 2 353 6 355 1.59e-48 171
Msa0610760 MtrunA17_Chr4g0069801 31.180 356 226 13 6 352 4 349 1.64e-48 171
Msa0610760 MtrunA17_Chr2g0290821 32.424 330 210 10 30 351 26 350 1.14e-47 169
Msa0610760 MtrunA17_Chr6g0461601 32.653 343 206 11 12 341 9 339 2.50e-47 168
Msa0610760 MtrunA17_Chr8g0383311 29.101 378 253 11 13 382 24 394 9.12e-46 164
Msa0610760 MtrunA17_Chr7g0253091 33.134 335 204 11 29 352 23 348 9.33e-46 164
Msa0610760 MtrunA17_Chr4g0076581 33.233 331 206 11 30 351 31 355 9.84e-44 159
Msa0610760 MtrunA17_Chr5g0436361 34.967 306 184 10 48 347 53 349 6.24e-42 151
Msa0610760 MtrunA17_Chr3g0105981 32.335 334 209 10 31 356 26 350 4.68e-41 150
Msa0610760 MtrunA17_Chr2g0306311 29.067 375 225 12 11 350 9 377 6.64e-40 149
Msa0610760 MtrunA17_Chr3g0129341 28.721 383 255 14 9 382 10 383 1.00e-38 145
Msa0610760 MtrunA17_Chr4g0018771 37.410 139 76 4 254 388 86 217 8.92e-21 90.1
Msa0610760 MtrunA17_Chr4g0018861 36.806 144 80 4 249 388 79 215 1.51e-20 89.4
Msa0610760 MtrunA17_Chr2g0294581 34.211 152 91 3 30 179 74 218 3.80e-17 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610760 AT4G18340.1 63.073 371 137 0 3 373 5 375 2.64e-176 497
Msa0610760 AT4G18340.2 62.634 372 139 0 3 374 5 376 7.27e-176 495
Msa0610760 AT1G30080.1 65.103 341 119 0 9 349 13 353 1.43e-168 478
Msa0610760 AT1G30080.2 64.306 353 122 2 1 349 1 353 1.46e-168 477
Msa0610760 AT2G27500.2 49.444 360 169 3 33 391 30 377 1.01e-122 360
Msa0610760 AT2G27500.1 50.729 343 162 2 33 374 30 366 8.08e-122 358
Msa0610760 AT1G32860.1 50.155 323 159 1 31 351 28 350 2.34e-120 356
Msa0610760 AT5G42100.2 51.692 325 154 2 29 350 24 348 6.44e-116 345
Msa0610760 AT5G42100.1 51.692 325 154 2 29 350 24 348 8.44e-116 344
Msa0610760 AT3G15800.1 46.089 358 180 3 29 384 40 386 2.86e-114 340
Msa0610760 AT2G26600.1 43.860 342 190 2 29 369 31 371 1.38e-105 317
Msa0610760 AT4G26830.1 48.286 350 172 5 12 359 8 350 7.25e-105 317
Msa0610760 AT4G26830.2 48.936 329 162 4 33 359 30 354 3.45e-103 313
Msa0610760 AT5G55180.1 48.297 323 164 3 32 352 27 348 6.93e-101 307
Msa0610760 AT5G55180.2 48.598 321 162 3 32 350 27 346 1.01e-100 307
Msa0610760 AT2G27500.3 50.340 294 139 2 82 374 1 288 1.11e-100 302
Msa0610760 AT5G24318.4 45.399 326 172 3 27 350 25 346 2.48e-97 295
Msa0610760 AT5G24318.2 43.526 363 193 6 27 382 25 382 9.50e-97 296
Msa0610760 AT5G24318.1 43.923 362 193 5 27 382 25 382 9.74e-97 296
Msa0610760 AT2G05790.1 45.963 322 171 3 29 348 22 342 8.05e-96 295
Msa0610760 AT4G34480.2 44.798 346 185 2 13 357 12 352 1.52e-94 288
Msa0610760 AT5G42720.1 42.133 375 212 3 9 381 3 374 9.58e-94 288
Msa0610760 AT4G34480.1 45.000 340 181 2 13 351 12 346 3.96e-92 286
Msa0610760 AT5G56590.1 43.769 329 180 3 22 348 19 344 1.22e-90 283
Msa0610760 AT2G16230.1 43.252 326 184 1 32 356 25 350 3.67e-90 281
Msa0610760 AT2G26600.2 45.221 272 148 1 98 369 7 277 9.47e-85 260
Msa0610760 AT3G07320.1 40.923 325 186 5 33 352 26 349 1.29e-84 265
Msa0610760 AT3G13560.4 40.683 322 186 3 29 346 23 343 3.14e-80 256
Msa0610760 AT3G13560.1 40.683 322 186 3 29 346 23 343 3.14e-80 256
Msa0610760 AT3G13560.2 40.683 322 186 3 29 346 23 343 3.14e-80 256
Msa0610760 AT3G13560.5 40.683 322 186 3 29 346 23 343 3.14e-80 256
Msa0610760 AT3G13560.3 40.683 322 186 3 29 346 23 343 3.14e-80 256
Msa0610760 AT4G14080.1 39.198 324 192 4 29 348 39 361 1.69e-77 248
Msa0610760 AT3G23770.1 38.507 335 198 5 25 352 30 363 4.83e-77 246
Msa0610760 AT1G11820.1 39.329 328 194 3 33 356 45 371 6.81e-77 243
Msa0610760 AT1G11820.3 39.628 323 190 3 33 351 45 366 6.30e-76 243
Msa0610760 AT1G11820.2 39.937 318 186 3 33 346 45 361 9.70e-76 244
Msa0610760 AT4G29360.1 42.236 322 184 2 26 346 21 341 1.87e-75 244
Msa0610760 AT4G29360.2 42.994 314 177 2 26 338 31 343 2.44e-75 244
Msa0610760 AT2G01630.2 40.123 324 189 3 32 351 23 345 2.74e-74 237
Msa0610760 AT2G01630.3 40.123 324 189 3 32 351 23 345 5.90e-74 237
Msa0610760 AT2G01630.1 40.123 324 189 3 32 351 23 345 2.94e-73 237
Msa0610760 AT3G55430.1 40.184 326 183 9 29 350 26 343 3.33e-72 233
Msa0610760 AT3G46570.1 41.515 330 182 8 26 350 21 344 1.35e-69 223
Msa0610760 AT1G66250.1 39.185 319 188 4 32 346 32 348 8.00e-68 223
Msa0610760 AT3G57270.1 38.871 319 183 8 33 348 28 337 1.30e-65 213
Msa0610760 AT5G20390.1 37.101 345 205 8 1 342 1 336 8.99e-65 211
Msa0610760 AT4G16260.1 34.870 347 213 6 8 352 1 336 4.74e-63 206
Msa0610760 AT5G20340.1 38.924 316 178 7 31 342 42 346 6.25e-63 206
Msa0610760 AT4G34480.3 47.210 233 123 0 119 351 3 235 8.33e-63 207
Msa0610760 AT3G61810.1 36.061 330 189 6 32 351 55 372 1.69e-61 203
Msa0610760 AT1G77780.1 34.483 348 215 9 17 362 8 344 2.37e-61 202
Msa0610760 AT2G39640.1 36.070 341 205 7 14 350 9 340 3.78e-61 207
Msa0610760 AT3G57260.1 36.686 338 196 10 13 348 15 336 5.92e-60 198
Msa0610760 AT3G57260.2 36.686 338 196 10 13 348 31 352 9.43e-60 198
Msa0610760 AT5G20330.1 38.291 316 180 7 31 342 33 337 2.00e-59 197
Msa0610760 AT4G16260.2 35.350 314 195 4 39 352 2 307 6.64e-59 194
Msa0610760 AT1G33220.1 37.574 338 188 9 14 338 8 335 8.75e-59 195
Msa0610760 AT5G24318.3 43.673 245 129 4 143 382 3 243 1.74e-58 193
Msa0610760 AT1G77790.1 34.493 345 213 8 7 348 7 341 1.03e-57 192
Msa0610760 AT3G57240.1 34.579 321 195 8 29 348 32 338 1.88e-52 178
Msa0610760 AT4G31140.1 31.180 356 228 11 4 352 3 348 1.50e-47 169
Msa0610760 AT3G24330.1 32.222 360 222 14 4 352 14 362 1.91e-47 169
Msa0610760 AT5G20560.1 31.642 335 211 9 11 340 15 336 2.61e-46 162
Msa0610760 AT5G64790.1 33.230 322 198 11 29 341 25 338 4.22e-45 162
Msa0610760 AT5G18220.1 32.229 332 212 8 27 352 25 349 2.60e-44 160
Msa0610760 AT1G64760.1 32.249 338 210 11 29 356 22 350 2.72e-42 155
Msa0610760 AT1G64760.2 32.249 338 210 11 29 356 22 350 2.72e-42 155
Msa0610760 AT2G19440.2 31.897 348 220 11 14 352 3 342 4.41e-42 154
Msa0610760 AT2G19440.1 31.897 348 220 11 14 352 3 342 4.41e-42 154
Msa0610760 AT5G58090.1 30.422 332 218 9 28 352 18 343 1.39e-41 153
Msa0610760 AT4G17180.1 30.514 331 210 11 30 351 23 342 1.65e-41 152
Msa0610760 AT5G20870.1 31.956 363 220 14 5 351 6 357 1.77e-41 153
Msa0610760 AT5G58480.1 31.498 327 209 9 29 347 23 342 2.79e-41 152
Msa0610760 AT3G04010.1 31.965 341 213 11 9 341 14 343 5.69e-41 151
Msa0610760 AT3G55780.1 32.075 318 200 10 46 355 49 358 1.09e-40 148

Find 66 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGAACAAGATATTGAATT+AGG 0.153002 4_2:+58631873 None:intergenic
ACATTTGCCATTGCTAAATT+AGG 0.171344 4_2:-58631515 Msa0610760:CDS
TATGATTCCAATCTCATTCT+AGG 0.189809 4_2:+58631344 None:intergenic
TCATAATGTAAATTTGTATT+AGG 0.242481 4_2:+58631569 None:intergenic
ATATTGATTTAGAGGGAAAT+AGG 0.284181 4_2:+58632419 None:intergenic
TTCGGACGTGTTGCGTCATT+TGG 0.288335 4_2:-58632193 Msa0610760:CDS
CTAAATAACTTGTATATTGT+TGG 0.296342 4_2:+58631105 None:intergenic
TAGTCTAGAGATATTTGATT+AGG 0.302492 4_2:+58631626 None:intergenic
TACAATGCTCTAGTTCAATT+AGG 0.318143 4_2:-58631836 Msa0610760:CDS
TGTGACAAATATTGATTTAG+AGG 0.321820 4_2:+58632411 None:intergenic
GCGTCATTTGGAATAAACTA+TGG 0.327515 4_2:-58632181 Msa0610760:CDS
TGCTCTAGTTCAATTAGGTT+TGG 0.329748 4_2:-58631831 Msa0610760:CDS
CTTGTTGTGGATCATTTAAT+TGG 0.341370 4_2:+58631996 None:intergenic
ATCTTTGTGTCTGGAAGATA+TGG 0.358675 4_2:+58631944 None:intergenic
GAGGTTAGGGTTTCTGAGAC+TGG 0.362945 4_2:-58631479 Msa0610760:CDS
AGTCTAGAGATATTTGATTA+GGG 0.367834 4_2:+58631627 None:intergenic
GTAAGAGAAATTGAAGTAGA+AGG 0.368771 4_2:+58631194 None:intergenic
GATGATCTCCACGTCTGAAT+TGG 0.369675 4_2:+58632045 None:intergenic
TTTGAATCCTAGAATGAGAT+TGG 0.371338 4_2:-58631351 Msa0610760:CDS
AAAGGTGACTCTGATGAAAT+TGG 0.380257 4_2:-58631446 Msa0610760:CDS
GGATCAACCATTCCTTGGTT+AGG 0.386797 4_2:+58631590 None:intergenic
TCATTCTTATAAGCAAAATA+AGG 0.388117 4_2:+58631650 None:intergenic
TTTAATGAAAATATGAAGAA+TGG 0.404108 4_2:-58631308 Msa0610760:CDS
TGGAACAAGATATTGAATTA+GGG 0.410253 4_2:+58631874 None:intergenic
TGGAAGATATGGTTTGATAT+TGG 0.415025 4_2:+58631955 None:intergenic
CCAACCTCAGAAAGGAACTA+TGG 0.427631 4_2:-58631284 Msa0610760:CDS
CCATAGTTCCTTTCTGAGGT+TGG 0.446684 4_2:+58631284 None:intergenic
TATTAGGATCAACCATTCCT+TGG 0.446836 4_2:+58631585 None:intergenic
ATCCACAACAAGCACTTCAA+TGG 0.448948 4_2:-58631985 Msa0610760:CDS
TGATAACATGCTTTATGCTA+TGG 0.465658 4_2:-58631549 Msa0610760:CDS
AGGATATGCATTGACCCAAA+AGG 0.470969 4_2:+58631670 None:intergenic
TTGTAAGTCATACTTTCATC+AGG 0.472826 4_2:+58631251 None:intergenic
AAAGTATGACTTACAATGTT+GGG 0.473433 4_2:-58631244 Msa0610760:CDS
ATTCCAGTGATCTTTGTGTC+TGG 0.474898 4_2:+58631935 None:intergenic
AAGATCACTGGAATTCAAGT+AGG 0.477367 4_2:-58631926 Msa0610760:CDS
GGCAACATTTACTCAAAGAA+TGG 0.488334 4_2:-58632386 Msa0610760:CDS
GGATATGCATTGACCCAAAA+GGG 0.491865 4_2:+58631671 None:intergenic
TTGTAAATGTTGATCACTGC+TGG 0.497136 4_2:+58631854 None:intergenic
TTTATAACAAGAATTTATTG+AGG 0.499075 4_2:-58631400 Msa0610760:CDS
TTAGGGTTTCTGAGACTGGT+TGG 0.504743 4_2:-58631475 Msa0610760:CDS
CTCTTACCTCTTCTGCCACT+AGG 0.508681 4_2:-58631178 Msa0610760:CDS
GTGACAAATATTGATTTAGA+GGG 0.511053 4_2:+58632412 None:intergenic
TCAAGAACTTCTAATGAACT+AGG 0.514280 4_2:+58631785 None:intergenic
AATCTCATTCTAGGATTCAA+AGG 0.515011 4_2:+58631353 None:intergenic
AGTTTCAGAAGTGAAATCTC+TGG 0.515867 4_2:-58631737 Msa0610760:CDS
GAATCTTACCCTCCTTCAGC+TGG 0.518663 4_2:-58631761 Msa0610760:CDS
GAGACTGGTTGGCCATCAAA+AGG 0.519791 4_2:-58631464 Msa0610760:CDS
TCCACAACAAGCACTTCAAT+GGG 0.523957 4_2:-58631984 Msa0610760:CDS
ATTTAATCCTAACCAAGGAA+TGG 0.526689 4_2:-58631597 Msa0610760:CDS
AGGAGACAAATGAAGAATGA+AGG 0.534907 4_2:-58631380 Msa0610760:CDS
CTTTGTCCTAGTGGCAGAAG+AGG 0.537253 4_2:+58631172 None:intergenic
CTTCCAGACACAAAGATCAC+TGG 0.544255 4_2:-58631938 Msa0610760:CDS
CTCTTCTGCCACTAGGACAA+AGG 0.544384 4_2:-58631171 Msa0610760:CDS
ACCCATTGAAGTGCTTGTTG+TGG 0.545635 4_2:+58631983 None:intergenic
GCGAAAGCTTTAAGAATCTG+TGG 0.549065 4_2:+58632067 None:intergenic
ATTAAAACCTAATTTAGCAA+TGG 0.564223 4_2:+58631508 None:intergenic
TAAATCAATATTTGTCACAA+TGG 0.569290 4_2:-58632407 Msa0610760:exon
CAAACCATAGTTCCTTTCTG+AGG 0.570996 4_2:+58631280 None:intergenic
GAAAGTATGACTTACAATGT+TGG 0.572069 4_2:-58631245 Msa0610760:CDS
AGATCACTGGAATTCAAGTA+GGG 0.581675 4_2:-58631925 Msa0610760:CDS
CTGAAACTACCAGCTGAAGG+AGG 0.582451 4_2:+58631752 None:intergenic
AGAATGGACCAACCTCAGAA+AGG 0.610841 4_2:-58631292 Msa0610760:CDS
AGCTTTCGCCAATTCAGACG+TGG 0.617283 4_2:-58632053 Msa0610760:CDS
GTATTATTTAATCCTAACCA+AGG 0.619362 4_2:-58631602 Msa0610760:CDS
TGAAACTACCAGCTGAAGGA+GGG 0.628259 4_2:+58631753 None:intergenic
CTTCTGAAACTACCAGCTGA+AGG 0.634495 4_2:+58631749 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATAATGTAAATTTGTATT+AGG + chr4_2:58631961-58631980 None:intergenic 15.0%
!! TTTATAACAAGAATTTATTG+AGG - chr4_2:58632127-58632146 Msa0610760:CDS 15.0%
!! TTTAATGAAAATATGAAGAA+TGG - chr4_2:58632219-58632238 Msa0610760:intron 15.0%
!! TCATAATGTAAATTTGTATT+AGG + chr4_2:58631961-58631980 None:intergenic 15.0%
!! TTTATAACAAGAATTTATTG+AGG - chr4_2:58632127-58632146 Msa0610760:CDS 15.0%
!! TTTAATGAAAATATGAAGAA+TGG - chr4_2:58632219-58632238 Msa0610760:intron 15.0%
!! TAAATCAATATTTGTCACAA+TGG - chr4_2:58631120-58631139 Msa0610760:CDS 20.0%
!!! ATTACAGATACAGAAATTTT+CGG - chr4_2:58631316-58631335 Msa0610760:CDS 20.0%
!! TCATTCTTATAAGCAAAATA+AGG + chr4_2:58631880-58631899 None:intergenic 20.0%
!! ATTAAAACCTAATTTAGCAA+TGG + chr4_2:58632022-58632041 None:intergenic 20.0%
!!! ATTGCTAAATTAGGTTTTAA+TGG - chr4_2:58632021-58632040 Msa0610760:CDS 20.0%
!!! TTGCTAAATTAGGTTTTAAT+GGG - chr4_2:58632022-58632041 Msa0610760:CDS 20.0%
!!! CTAAATAACTTGTATATTGT+TGG + chr4_2:58632425-58632444 None:intergenic 20.0%
!! TAAATCAATATTTGTCACAA+TGG - chr4_2:58631120-58631139 Msa0610760:CDS 20.0%
!!! ATTACAGATACAGAAATTTT+CGG - chr4_2:58631316-58631335 Msa0610760:CDS 20.0%
!! TCATTCTTATAAGCAAAATA+AGG + chr4_2:58631880-58631899 None:intergenic 20.0%
!! ATTAAAACCTAATTTAGCAA+TGG + chr4_2:58632022-58632041 None:intergenic 20.0%
!!! ATTGCTAAATTAGGTTTTAA+TGG - chr4_2:58632021-58632040 Msa0610760:CDS 20.0%
!!! TTGCTAAATTAGGTTTTAAT+GGG - chr4_2:58632022-58632041 Msa0610760:CDS 20.0%
!!! CTAAATAACTTGTATATTGT+TGG + chr4_2:58632425-58632444 None:intergenic 20.0%
!!! ATATTGATTTAGAGGGAAAT+AGG + chr4_2:58631111-58631130 None:intergenic 25.0%
!!! GTGACAAATATTGATTTAGA+GGG + chr4_2:58631118-58631137 None:intergenic 25.0%
!!! TGTGACAAATATTGATTTAG+AGG + chr4_2:58631119-58631138 None:intergenic 25.0%
! TGCATGATGAAAAGAATAAT+AGG + chr4_2:58631231-58631250 None:intergenic 25.0%
!!! TCTTGTTTTTGTGATCTTTA+AGG + chr4_2:58631425-58631444 None:intergenic 25.0%
! TGGAACAAGATATTGAATTA+GGG + chr4_2:58631656-58631675 None:intergenic 25.0%
!! AAAAACAAAATCACCCTTTT+GGG - chr4_2:58631843-58631862 Msa0610760:CDS 25.0%
! AGTCTAGAGATATTTGATTA+GGG + chr4_2:58631903-58631922 None:intergenic 25.0%
! TAGTCTAGAGATATTTGATT+AGG + chr4_2:58631904-58631923 None:intergenic 25.0%
! GTATTATTTAATCCTAACCA+AGG - chr4_2:58631925-58631944 Msa0610760:CDS 25.0%
! AAAGTATGACTTACAATGTT+GGG - chr4_2:58632283-58632302 Msa0610760:intron 25.0%
!!! ATATTGATTTAGAGGGAAAT+AGG + chr4_2:58631111-58631130 None:intergenic 25.0%
!!! GTGACAAATATTGATTTAGA+GGG + chr4_2:58631118-58631137 None:intergenic 25.0%
!!! TGTGACAAATATTGATTTAG+AGG + chr4_2:58631119-58631138 None:intergenic 25.0%
! TGCATGATGAAAAGAATAAT+AGG + chr4_2:58631231-58631250 None:intergenic 25.0%
!!! TCTTGTTTTTGTGATCTTTA+AGG + chr4_2:58631425-58631444 None:intergenic 25.0%
! TGGAACAAGATATTGAATTA+GGG + chr4_2:58631656-58631675 None:intergenic 25.0%
!! AAAAACAAAATCACCCTTTT+GGG - chr4_2:58631843-58631862 Msa0610760:CDS 25.0%
! AGTCTAGAGATATTTGATTA+GGG + chr4_2:58631903-58631922 None:intergenic 25.0%
! TAGTCTAGAGATATTTGATT+AGG + chr4_2:58631904-58631923 None:intergenic 25.0%
! GTATTATTTAATCCTAACCA+AGG - chr4_2:58631925-58631944 Msa0610760:CDS 25.0%
! AAAGTATGACTTACAATGTT+GGG - chr4_2:58632283-58632302 Msa0610760:intron 25.0%
GTGAAAAAACAGAACAAACA+GGG + chr4_2:58631209-58631228 None:intergenic 30.0%
! CTTGTTGTGGATCATTTAAT+TGG + chr4_2:58631534-58631553 None:intergenic 30.0%
TGGAAGATATGGTTTGATAT+TGG + chr4_2:58631575-58631594 None:intergenic 30.0%
CTGGAACAAGATATTGAATT+AGG + chr4_2:58631657-58631676 None:intergenic 30.0%
! TACAATGCTCTAGTTCAATT+AGG - chr4_2:58631691-58631710 Msa0610760:CDS 30.0%
TCAAGAACTTCTAATGAACT+AGG + chr4_2:58631745-58631764 None:intergenic 30.0%
! CAAAAACAAAATCACCCTTT+TGG - chr4_2:58631842-58631861 Msa0610760:CDS 30.0%
ATTTAATCCTAACCAAGGAA+TGG - chr4_2:58631930-58631949 Msa0610760:CDS 30.0%
TGATAACATGCTTTATGCTA+TGG - chr4_2:58631978-58631997 Msa0610760:CDS 30.0%
ACATTTGCCATTGCTAAATT+AGG - chr4_2:58632012-58632031 Msa0610760:CDS 30.0%
!! ATTAGGTTTTAATGGGATAG+AGG - chr4_2:58632029-58632048 Msa0610760:CDS 30.0%
! TTTTAATGGGATAGAGGTTA+GGG - chr4_2:58632035-58632054 Msa0610760:CDS 30.0%
!! AATCTCATTCTAGGATTCAA+AGG + chr4_2:58632177-58632196 None:intergenic 30.0%
TTTGAATCCTAGAATGAGAT+TGG - chr4_2:58632176-58632195 Msa0610760:CDS 30.0%
TATGATTCCAATCTCATTCT+AGG + chr4_2:58632186-58632205 None:intergenic 30.0%
TTGTAAGTCATACTTTCATC+AGG + chr4_2:58632279-58632298 None:intergenic 30.0%
! GAAAGTATGACTTACAATGT+TGG - chr4_2:58632282-58632301 Msa0610760:intron 30.0%
GTAAGAGAAATTGAAGTAGA+AGG + chr4_2:58632336-58632355 None:intergenic 30.0%
!!! TTTTTTTACCTTTGTCCTAG+TGG + chr4_2:58632367-58632386 None:intergenic 30.0%
GTGAAAAAACAGAACAAACA+GGG + chr4_2:58631209-58631228 None:intergenic 30.0%
! CTTGTTGTGGATCATTTAAT+TGG + chr4_2:58631534-58631553 None:intergenic 30.0%
TGGAAGATATGGTTTGATAT+TGG + chr4_2:58631575-58631594 None:intergenic 30.0%
CTGGAACAAGATATTGAATT+AGG + chr4_2:58631657-58631676 None:intergenic 30.0%
! TACAATGCTCTAGTTCAATT+AGG - chr4_2:58631691-58631710 Msa0610760:CDS 30.0%
TCAAGAACTTCTAATGAACT+AGG + chr4_2:58631745-58631764 None:intergenic 30.0%
! CAAAAACAAAATCACCCTTT+TGG - chr4_2:58631842-58631861 Msa0610760:CDS 30.0%
ATTTAATCCTAACCAAGGAA+TGG - chr4_2:58631930-58631949 Msa0610760:CDS 30.0%
TGATAACATGCTTTATGCTA+TGG - chr4_2:58631978-58631997 Msa0610760:CDS 30.0%
ACATTTGCCATTGCTAAATT+AGG - chr4_2:58632012-58632031 Msa0610760:CDS 30.0%
!! ATTAGGTTTTAATGGGATAG+AGG - chr4_2:58632029-58632048 Msa0610760:CDS 30.0%
! TTTTAATGGGATAGAGGTTA+GGG - chr4_2:58632035-58632054 Msa0610760:CDS 30.0%
!! AATCTCATTCTAGGATTCAA+AGG + chr4_2:58632177-58632196 None:intergenic 30.0%
TTTGAATCCTAGAATGAGAT+TGG - chr4_2:58632176-58632195 Msa0610760:CDS 30.0%
TATGATTCCAATCTCATTCT+AGG + chr4_2:58632186-58632205 None:intergenic 30.0%
TTGTAAGTCATACTTTCATC+AGG + chr4_2:58632279-58632298 None:intergenic 30.0%
! GAAAGTATGACTTACAATGT+TGG - chr4_2:58632282-58632301 Msa0610760:intron 30.0%
GTAAGAGAAATTGAAGTAGA+AGG + chr4_2:58632336-58632355 None:intergenic 30.0%
!!! TTTTTTTACCTTTGTCCTAG+TGG + chr4_2:58632367-58632386 None:intergenic 30.0%
GGCAACATTTACTCAAAGAA+TGG - chr4_2:58631141-58631160 Msa0610760:CDS 35.0%
GGTGAAAAAACAGAACAAAC+AGG + chr4_2:58631210-58631229 None:intergenic 35.0%
! GCGTCATTTGGAATAAACTA+TGG - chr4_2:58631346-58631365 Msa0610760:CDS 35.0%
!! CACAACCAGAAAAAGTACTA+GGG - chr4_2:58631389-58631408 Msa0610760:CDS 35.0%
ATCTTTGTGTCTGGAAGATA+TGG + chr4_2:58631586-58631605 None:intergenic 35.0%
AAGATCACTGGAATTCAAGT+AGG - chr4_2:58631601-58631620 Msa0610760:CDS 35.0%
AGATCACTGGAATTCAAGTA+GGG - chr4_2:58631602-58631621 Msa0610760:CDS 35.0%
TTGTAAATGTTGATCACTGC+TGG + chr4_2:58631676-58631695 None:intergenic 35.0%
! TGCTCTAGTTCAATTAGGTT+TGG - chr4_2:58631696-58631715 Msa0610760:CDS 35.0%
AGTTTCAGAAGTGAAATCTC+TGG - chr4_2:58631790-58631809 Msa0610760:CDS 35.0%
TATTAGGATCAACCATTCCT+TGG + chr4_2:58631945-58631964 None:intergenic 35.0%
!! GTTTTAATGGGATAGAGGTT+AGG - chr4_2:58632034-58632053 Msa0610760:CDS 35.0%
AAAGGTGACTCTGATGAAAT+TGG - chr4_2:58632081-58632100 Msa0610760:CDS 35.0%
AGGAGACAAATGAAGAATGA+AGG - chr4_2:58632147-58632166 Msa0610760:CDS 35.0%
GGCAACATTTACTCAAAGAA+TGG - chr4_2:58631141-58631160 Msa0610760:CDS 35.0%
GGTGAAAAAACAGAACAAAC+AGG + chr4_2:58631210-58631229 None:intergenic 35.0%
! GCGTCATTTGGAATAAACTA+TGG - chr4_2:58631346-58631365 Msa0610760:CDS 35.0%
!! CACAACCAGAAAAAGTACTA+GGG - chr4_2:58631389-58631408 Msa0610760:CDS 35.0%
ATCTTTGTGTCTGGAAGATA+TGG + chr4_2:58631586-58631605 None:intergenic 35.0%
AAGATCACTGGAATTCAAGT+AGG - chr4_2:58631601-58631620 Msa0610760:CDS 35.0%
AGATCACTGGAATTCAAGTA+GGG - chr4_2:58631602-58631621 Msa0610760:CDS 35.0%
TTGTAAATGTTGATCACTGC+TGG + chr4_2:58631676-58631695 None:intergenic 35.0%
! TGCTCTAGTTCAATTAGGTT+TGG - chr4_2:58631696-58631715 Msa0610760:CDS 35.0%
AGTTTCAGAAGTGAAATCTC+TGG - chr4_2:58631790-58631809 Msa0610760:CDS 35.0%
TATTAGGATCAACCATTCCT+TGG + chr4_2:58631945-58631964 None:intergenic 35.0%
!! GTTTTAATGGGATAGAGGTT+AGG - chr4_2:58632034-58632053 Msa0610760:CDS 35.0%
AAAGGTGACTCTGATGAAAT+TGG - chr4_2:58632081-58632100 Msa0610760:CDS 35.0%
AGGAGACAAATGAAGAATGA+AGG - chr4_2:58632147-58632166 Msa0610760:CDS 35.0%
!!! CTAGCATTTTTTCTCTCTGC+AGG - chr4_2:58631181-58631200 Msa0610760:CDS 40.0%
!!! CCTAGTACTTTTTCTGGTTG+TGG + chr4_2:58631391-58631410 None:intergenic 40.0%
!! CCACAACCAGAAAAAGTACT+AGG - chr4_2:58631388-58631407 Msa0610760:CDS 40.0%
!!! AGAAGCCCTAGTACTTTTTC+TGG + chr4_2:58631397-58631416 None:intergenic 40.0%
!! GCGAAAGCTTTAAGAATCTG+TGG + chr4_2:58631463-58631482 None:intergenic 40.0%
ATCCACAACAAGCACTTCAA+TGG - chr4_2:58631542-58631561 Msa0610760:CDS 40.0%
TCCACAACAAGCACTTCAAT+GGG - chr4_2:58631543-58631562 Msa0610760:CDS 40.0%
ATTCCAGTGATCTTTGTGTC+TGG + chr4_2:58631595-58631614 None:intergenic 40.0%
! GGATATGCATTGACCCAAAA+GGG + chr4_2:58631859-58631878 None:intergenic 40.0%
! AGGATATGCATTGACCCAAA+AGG + chr4_2:58631860-58631879 None:intergenic 40.0%
! TCATCAGAGTCACCTTTTGA+TGG + chr4_2:58632078-58632097 None:intergenic 40.0%
CAAACCATAGTTCCTTTCTG+AGG + chr4_2:58632250-58632269 None:intergenic 40.0%
!!! CTAGCATTTTTTCTCTCTGC+AGG - chr4_2:58631181-58631200 Msa0610760:CDS 40.0%
!!! CCTAGTACTTTTTCTGGTTG+TGG + chr4_2:58631391-58631410 None:intergenic 40.0%
!! CCACAACCAGAAAAAGTACT+AGG - chr4_2:58631388-58631407 Msa0610760:CDS 40.0%
!!! AGAAGCCCTAGTACTTTTTC+TGG + chr4_2:58631397-58631416 None:intergenic 40.0%
!! GCGAAAGCTTTAAGAATCTG+TGG + chr4_2:58631463-58631482 None:intergenic 40.0%
ATCCACAACAAGCACTTCAA+TGG - chr4_2:58631542-58631561 Msa0610760:CDS 40.0%
TCCACAACAAGCACTTCAAT+GGG - chr4_2:58631543-58631562 Msa0610760:CDS 40.0%
ATTCCAGTGATCTTTGTGTC+TGG + chr4_2:58631595-58631614 None:intergenic 40.0%
! GGATATGCATTGACCCAAAA+GGG + chr4_2:58631859-58631878 None:intergenic 40.0%
! AGGATATGCATTGACCCAAA+AGG + chr4_2:58631860-58631879 None:intergenic 40.0%
! TCATCAGAGTCACCTTTTGA+TGG + chr4_2:58632078-58632097 None:intergenic 40.0%
CAAACCATAGTTCCTTTCTG+AGG + chr4_2:58632250-58632269 None:intergenic 40.0%
GATGATCTCCACGTCTGAAT+TGG + chr4_2:58631485-58631504 None:intergenic 45.0%
! ACCCATTGAAGTGCTTGTTG+TGG + chr4_2:58631547-58631566 None:intergenic 45.0%
CTTCCAGACACAAAGATCAC+TGG - chr4_2:58631589-58631608 Msa0610760:CDS 45.0%
TGAAACTACCAGCTGAAGGA+GGG + chr4_2:58631777-58631796 None:intergenic 45.0%
!! CTTCTGAAACTACCAGCTGA+AGG + chr4_2:58631781-58631800 None:intergenic 45.0%
GGATCAACCATTCCTTGGTT+AGG + chr4_2:58631940-58631959 None:intergenic 45.0%
!! TTAGGGTTTCTGAGACTGGT+TGG - chr4_2:58632052-58632071 Msa0610760:CDS 45.0%
AGAATGGACCAACCTCAGAA+AGG - chr4_2:58632235-58632254 Msa0610760:intron 45.0%
!! CCATAGTTCCTTTCTGAGGT+TGG + chr4_2:58632246-58632265 None:intergenic 45.0%
CCAACCTCAGAAAGGAACTA+TGG - chr4_2:58632243-58632262 Msa0610760:intron 45.0%
GATGATCTCCACGTCTGAAT+TGG + chr4_2:58631485-58631504 None:intergenic 45.0%
! ACCCATTGAAGTGCTTGTTG+TGG + chr4_2:58631547-58631566 None:intergenic 45.0%
CTTCCAGACACAAAGATCAC+TGG - chr4_2:58631589-58631608 Msa0610760:CDS 45.0%
TGAAACTACCAGCTGAAGGA+GGG + chr4_2:58631777-58631796 None:intergenic 45.0%
!! CTTCTGAAACTACCAGCTGA+AGG + chr4_2:58631781-58631800 None:intergenic 45.0%
GGATCAACCATTCCTTGGTT+AGG + chr4_2:58631940-58631959 None:intergenic 45.0%
!! TTAGGGTTTCTGAGACTGGT+TGG - chr4_2:58632052-58632071 Msa0610760:CDS 45.0%
AGAATGGACCAACCTCAGAA+AGG - chr4_2:58632235-58632254 Msa0610760:intron 45.0%
!! CCATAGTTCCTTTCTGAGGT+TGG + chr4_2:58632246-58632265 None:intergenic 45.0%
CCAACCTCAGAAAGGAACTA+TGG - chr4_2:58632243-58632262 Msa0610760:intron 45.0%
TTCGGACGTGTTGCGTCATT+TGG - chr4_2:58631334-58631353 Msa0610760:CDS 50.0%
AGCTTTCGCCAATTCAGACG+TGG - chr4_2:58631474-58631493 Msa0610760:CDS 50.0%
GAATCTTACCCTCCTTCAGC+TGG - chr4_2:58631766-58631785 Msa0610760:CDS 50.0%
CTGAAACTACCAGCTGAAGG+AGG + chr4_2:58631778-58631797 None:intergenic 50.0%
!! GAGGTTAGGGTTTCTGAGAC+TGG - chr4_2:58632048-58632067 Msa0610760:CDS 50.0%
! GAGACTGGTTGGCCATCAAA+AGG - chr4_2:58632063-58632082 Msa0610760:CDS 50.0%
CTCTTACCTCTTCTGCCACT+AGG - chr4_2:58632349-58632368 Msa0610760:CDS 50.0%
CTTTGTCCTAGTGGCAGAAG+AGG + chr4_2:58632358-58632377 None:intergenic 50.0%
CTCTTCTGCCACTAGGACAA+AGG - chr4_2:58632356-58632375 Msa0610760:CDS 50.0%
TTCGGACGTGTTGCGTCATT+TGG - chr4_2:58631334-58631353 Msa0610760:CDS 50.0%
AGCTTTCGCCAATTCAGACG+TGG - chr4_2:58631474-58631493 Msa0610760:CDS 50.0%
GAATCTTACCCTCCTTCAGC+TGG - chr4_2:58631766-58631785 Msa0610760:CDS 50.0%
CTGAAACTACCAGCTGAAGG+AGG + chr4_2:58631778-58631797 None:intergenic 50.0%
!! GAGGTTAGGGTTTCTGAGAC+TGG - chr4_2:58632048-58632067 Msa0610760:CDS 50.0%
! GAGACTGGTTGGCCATCAAA+AGG - chr4_2:58632063-58632082 Msa0610760:CDS 50.0%
CTCTTACCTCTTCTGCCACT+AGG - chr4_2:58632349-58632368 Msa0610760:CDS 50.0%
CTTTGTCCTAGTGGCAGAAG+AGG + chr4_2:58632358-58632377 None:intergenic 50.0%
CTCTTCTGCCACTAGGACAA+AGG - chr4_2:58632356-58632375 Msa0610760:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 58631085 58632464 58631085 ID=Msa0610760;Name=Msa0610760
chr4_2 mRNA 58631085 58632464 58631085 ID=Msa0610760-mRNA-1;Parent=Msa0610760;Name=Msa0610760-mRNA-1;_AED=0.09;_eAED=0.09;_QI=54|1|0.5|1|1|1|2|0|401
chr4_2 exon 58631085 58632226 58631085 ID=Msa0610760-mRNA-1:exon:15462;Parent=Msa0610760-mRNA-1
chr4_2 exon 58632347 58632464 58632347 ID=Msa0610760-mRNA-1:exon:15461;Parent=Msa0610760-mRNA-1
chr4_2 five_prime_UTR 58632411 58632464 58632411 ID=Msa0610760-mRNA-1:five_prime_utr;Parent=Msa0610760-mRNA-1
chr4_2 CDS 58632347 58632410 58632347 ID=Msa0610760-mRNA-1:cds;Parent=Msa0610760-mRNA-1
chr4_2 CDS 58631085 58632226 58631085 ID=Msa0610760-mRNA-1:cds;Parent=Msa0610760-mRNA-1
Gene Sequence

>Msa0610760

ATGGCAACATTTACTCAAAGAATGGCAATATGTTATATTCTTCTAGCATTTTTTCTCTCTGCAGATACAGAAATTTTCGGACGTGTTGCGTCATTTGGAATAAACTATGGACAAGTAGCTAACAATTTGCCACAACCAGAAAAAGTACTAGGGCTTCTCAGCACCTTAAAGATCACAAAAACAAGAATCTACGATACGAATCCACAGATTCTTAAAGCTTTCGCCAATTCAGACGTGGAGATCATCGTAACAATTGAAAATCAAATACTAAACCAATTAAATGATCCACAACAAGCACTTCAATGGGTAAATACCAATATCAAACCATATCTTCCAGACACAAAGATCACTGGAATTCAAGTAGGGAATGAAGTGTTCACAGAACTAGACACAACCCTAATTCAATATCTTGTTCCAGCAGTGATCAACATTTACAATGCTCTAGTTCAATTAGGTTTGGACTCAAACATTCACGTGTCAACACCTAGTTCATTAGAAGTTCTTGAAGAATCTTACCCTCCTTCAGCTGGTAGTTTCAGAAGTGAAATCTCTGGAATCATGCATGATTTTTTGAACTTTTTGTCAAAAACAAAATCACCCTTTTGGGTCAATGCATATCCTTATTTTGCTTATAAGAATGACCCTAATCAAATATCTCTAGACTATGTATTATTTAATCCTAACCAAGGAATGGTTGATCCTAATACAAATTTACATTATGATAACATGCTTTATGCTATGGTAGATGCTGTTACATTTGCCATTGCTAAATTAGGTTTTAATGGGATAGAGGTTAGGGTTTCTGAGACTGGTTGGCCATCAAAAGGTGACTCTGATGAAATTGGTGCATCATTGAAAAATGCTGAAATTTATAACAAGAATTTATTGAGGAGACAAATGAAGAATGAAGGAACACCTTTGAATCCTAGAATGAGATTGGAATCATATTTGTTTGCATTATTTAATGAAAATATGAAGAATGGACCAACCTCAGAAAGGAACTATGGTTTGTTTGAGCCTGATGAAAGTATGACTTACAATGTTGGGTTGTCTTCTTTTGCAAAGTCATCAAATCCTTCTACTTCAATTTCTCTTACCTCTTCTGCCACTAGGACAAAGGTAAAAAAAAAAAGAGTAAAGACATTGTTTTTTTTCTTTCTTTTCCAACAATATACAAGTTATTTAGCTTATAGTAAAATTTTGTGA
Protein sequence

>Msa0610760

MATFTQRMAICYILLAFFLSADTEIFGRVASFGINYGQVANNLPQPEKVLGLLSTLKITKTRIYDTNPQILKAFANSDVEIIVTIENQILNQLNDPQQALQWVNTNIKPYLPDTKITGIQVGNEVFTELDTTLIQYLVPAVINIYNALVQLGLDSNIHVSTPSSLEVLEESYPPSAGSFRSEISGIMHDFLNFLSKTKSPFWVNAYPYFAYKNDPNQISLDYVLFNPNQGMVDPNTNLHYDNMLYAMVDAVTFAIAKLGFNGIEVRVSETGWPSKGDSDEIGASLKNAEIYNKNLLRRQMKNEGTPLNPRMRLESYLFALFNENMKNGPTSERNYGLFEPDESMTYNVGLSSFAKSSNPSTSISLTSSATRTKVKKKRVKTLFFFFLFQQYTSYLAYSKIL*