AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1437520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437520 MtrunA17_Chr4g0043851 93.523 386 25 0 1 386 1 386 0.0 738
Msa1437520 MtrunA17_Chr2g0285901 76.106 339 75 2 12 350 9 341 0.0 538
Msa1437520 MtrunA17_Chr1g0189111 56.231 329 143 1 23 351 22 349 8.18e-136 395
Msa1437520 MtrunA17_Chr4g0026321 52.632 323 153 0 31 353 28 350 4.05e-126 369
Msa1437520 MtrunA17_Chr2g0321511 49.563 343 170 2 9 350 5 345 4.69e-123 361
Msa1437520 MtrunA17_Chr4g0003281 48.368 337 173 1 30 365 38 374 5.04e-117 346
Msa1437520 MtrunA17_Chr4g0064021 45.846 325 175 1 30 353 24 348 1.05e-102 309
Msa1437520 MtrunA17_Chr5g0439251 49.231 325 162 3 29 351 22 345 1.45e-99 304
Msa1437520 MtrunA17_Chr3g0144531 43.407 364 199 3 1 362 1 359 7.21e-99 301
Msa1437520 MtrunA17_Chr1g0155001 46.584 322 170 2 32 351 24 345 3.84e-97 296
Msa1437520 MtrunA17_Chr4g0034901 44.444 351 193 2 14 362 10 360 7.21e-97 296
Msa1437520 MtrunA17_Chr1g0177171 45.706 326 174 3 28 351 34 358 3.84e-95 293
Msa1437520 MtrunA17_Chr3g0119541 45.294 340 178 4 30 362 24 362 6.78e-95 288
Msa1437520 MtrunA17_Chr1g0146831 45.846 325 170 3 25 347 24 344 1.96e-93 285
Msa1437520 MtrunA17_Chr8g0389741 42.609 345 189 3 33 376 29 365 1.26e-89 279
Msa1437520 MtrunA17_Chr6g0487261 45.312 320 173 2 33 351 28 346 1.08e-87 275
Msa1437520 MtrunA17_Chr7g0223961 44.753 324 171 5 29 350 20 337 1.71e-86 270
Msa1437520 MtrunA17_Chr7g0234821 42.462 325 181 5 33 352 27 350 2.93e-86 269
Msa1437520 MtrunA17_Chr8g0339191 38.655 357 210 6 1 352 1 353 3.03e-82 259
Msa1437520 MtrunA17_Chr4g0046311 39.394 330 192 5 30 352 21 349 1.49e-78 249
Msa1437520 MtrunA17_Chr4g0043511 41.667 348 194 7 7 350 37 379 1.16e-76 247
Msa1437520 MtrunA17_Chr3g0121271 37.576 330 200 4 14 338 7 335 2.09e-76 245
Msa1437520 MtrunA17_Chr4g0075031 39.144 327 194 3 32 354 45 370 2.57e-75 243
Msa1437520 MtrunA17_Chr8g0378621 37.383 321 196 3 30 346 24 343 7.32e-74 238
Msa1437520 MtrunA17_Chr1g0161011 38.506 348 208 4 8 351 1 346 1.17e-73 238
Msa1437520 MtrunA17_Chr4g0039011 40.123 324 182 6 30 353 40 351 2.51e-73 233
Msa1437520 MtrunA17_Chr5g0434591 37.892 351 205 5 8 351 1 345 1.26e-72 235
Msa1437520 MtrunA17_Chr3g0110371 36.826 334 206 3 10 339 2 334 1.62e-69 227
Msa1437520 MtrunA17_Chr4g0038981 38.053 339 195 9 8 346 1 324 9.83e-67 215
Msa1437520 MtrunA17_Chr4g0038971 37.537 341 198 10 8 348 1 326 1.09e-66 214
Msa1437520 MtrunA17_Chr4g0038961 37.719 342 196 10 8 348 1 326 3.60e-66 213
Msa1437520 MtrunA17_Chr4g0038941 39.009 323 184 6 26 348 46 355 2.16e-65 212
Msa1437520 MtrunA17_Chr2g0294601 38.769 325 189 7 30 352 57 373 2.14e-63 208
Msa1437520 MtrunA17_Chr4g0039071 38.415 328 192 7 24 349 17 336 3.00e-63 207
Msa1437520 MtrunA17_Chr7g0250301 34.365 323 202 6 25 346 1 314 1.05e-62 204
Msa1437520 MtrunA17_Chr2g0294591 35.241 332 203 8 30 358 28 350 2.31e-58 194
Msa1437520 MtrunA17_Chr1g0174261 38.754 289 167 6 62 348 2 282 1.56e-57 191
Msa1437520 MtrunA17_Chr4g0039021 37.676 284 167 5 58 340 7 281 1.64e-55 184
Msa1437520 MtrunA17_Chr1g0148391 33.989 356 214 13 6 352 4 347 1.89e-52 181
Msa1437520 MtrunA17_Chr7g0250321 33.219 292 184 6 7 297 5 286 6.26e-51 172
Msa1437520 MtrunA17_Chr3g0138741 31.831 355 226 11 5 352 7 352 6.39e-49 172
Msa1437520 MtrunA17_Chr4g0062771 32.231 363 222 14 2 353 6 355 1.56e-48 171
Msa1437520 MtrunA17_Chr4g0069801 31.180 356 226 13 6 352 4 349 2.36e-48 171
Msa1437520 MtrunA17_Chr2g0290821 32.530 332 209 10 30 351 26 352 6.35e-48 170
Msa1437520 MtrunA17_Chr6g0461601 32.653 343 206 11 12 341 9 339 2.20e-47 168
Msa1437520 MtrunA17_Chr7g0253091 33.134 335 204 11 29 352 23 348 1.14e-45 163
Msa1437520 MtrunA17_Chr8g0383311 29.577 355 235 11 13 359 24 371 7.00e-45 162
Msa1437520 MtrunA17_Chr4g0076581 33.233 331 206 11 30 351 31 355 7.38e-44 159
Msa1437520 MtrunA17_Chr5g0436361 34.967 306 184 10 48 347 53 349 6.14e-42 151
Msa1437520 MtrunA17_Chr3g0105981 32.335 334 209 10 31 356 26 350 2.87e-41 150
Msa1437520 MtrunA17_Chr2g0306311 29.067 375 225 12 11 350 9 377 5.12e-40 149
Msa1437520 MtrunA17_Chr3g0129341 29.477 363 238 14 9 362 10 363 2.82e-38 144
Msa1437520 MtrunA17_Chr4g0018771 43.519 108 54 3 254 361 86 186 8.53e-20 87.4
Msa1437520 MtrunA17_Chr4g0018861 42.478 113 58 3 249 361 79 184 1.34e-19 86.7
Msa1437520 MtrunA17_Chr2g0294581 33.553 152 92 3 30 179 74 218 4.50e-17 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437520 AT4G18340.1 63.073 371 137 0 3 373 5 375 3.19e-176 497
Msa1437520 AT4G18340.2 62.634 372 139 0 3 374 5 376 6.82e-176 495
Msa1437520 AT1G30080.2 64.306 353 122 2 1 349 1 353 1.32e-168 477
Msa1437520 AT1G30080.1 65.103 341 119 0 9 349 13 353 2.20e-168 477
Msa1437520 AT2G27500.2 51.020 343 161 2 33 374 30 366 1.52e-122 360
Msa1437520 AT2G27500.1 50.729 343 162 2 33 374 30 366 8.73e-122 358
Msa1437520 AT1G32860.1 50.155 323 159 1 31 351 28 350 2.01e-120 356
Msa1437520 AT5G42100.2 51.692 325 154 2 29 350 24 348 6.11e-116 344
Msa1437520 AT5G42100.1 51.692 325 154 2 29 350 24 348 8.27e-116 344
Msa1437520 AT3G15800.1 48.036 331 170 2 29 357 40 370 4.23e-114 339
Msa1437520 AT2G26600.1 43.860 342 190 2 29 369 31 371 1.25e-105 317
Msa1437520 AT4G26830.1 48.286 350 172 5 12 359 8 350 8.11e-105 317
Msa1437520 AT4G26830.2 48.936 329 162 4 33 359 30 354 4.95e-103 313
Msa1437520 AT5G55180.1 48.297 323 164 3 32 352 27 348 1.02e-100 307
Msa1437520 AT2G27500.3 50.340 294 139 2 82 374 1 288 1.03e-100 301
Msa1437520 AT5G55180.2 48.598 321 162 3 32 350 27 346 1.22e-100 307
Msa1437520 AT5G24318.4 45.399 326 172 3 27 350 25 346 3.04e-97 295
Msa1437520 AT5G24318.2 43.407 364 196 5 27 384 25 384 1.85e-96 296
Msa1437520 AT5G24318.1 43.407 364 196 5 27 384 25 384 2.35e-96 295
Msa1437520 AT2G05790.1 45.963 322 171 3 29 348 22 342 6.53e-96 295
Msa1437520 AT4G34480.2 44.798 346 185 2 13 357 12 352 1.58e-94 287
Msa1437520 AT5G42720.1 44.058 345 191 2 9 351 3 347 1.10e-93 288
Msa1437520 AT4G34480.1 45.000 340 181 2 13 351 12 346 5.34e-92 286
Msa1437520 AT5G56590.1 43.373 332 183 3 22 351 19 347 6.61e-91 283
Msa1437520 AT2G16230.1 43.750 320 179 1 33 351 26 345 3.58e-90 281
Msa1437520 AT2G26600.2 45.221 272 148 1 98 369 7 277 1.03e-84 260
Msa1437520 AT3G07320.1 41.231 325 185 5 33 352 26 349 1.53e-84 265
Msa1437520 AT3G13560.4 40.683 322 186 3 29 346 23 343 2.44e-80 256
Msa1437520 AT3G13560.1 40.683 322 186 3 29 346 23 343 2.44e-80 256
Msa1437520 AT3G13560.2 40.683 322 186 3 29 346 23 343 2.44e-80 256
Msa1437520 AT3G13560.5 40.683 322 186 3 29 346 23 343 2.44e-80 256
Msa1437520 AT3G13560.3 40.683 322 186 3 29 346 23 343 2.44e-80 256
Msa1437520 AT4G14080.1 39.198 324 192 4 29 348 39 361 2.47e-77 247
Msa1437520 AT1G11820.1 39.329 328 194 3 33 356 45 371 4.80e-77 243
Msa1437520 AT3G23770.1 38.507 335 198 5 25 352 30 363 5.18e-77 246
Msa1437520 AT1G11820.3 39.628 323 190 3 33 351 45 366 4.60e-76 244
Msa1437520 AT1G11820.2 39.937 318 186 3 33 346 45 361 9.05e-76 244
Msa1437520 AT4G29360.2 42.236 322 184 2 26 346 31 351 1.46e-75 244
Msa1437520 AT4G29360.1 42.236 322 184 2 26 346 21 341 1.47e-75 244
Msa1437520 AT2G01630.2 40.123 324 189 3 32 351 23 345 2.42e-74 237
Msa1437520 AT2G01630.3 40.123 324 189 3 32 351 23 345 4.90e-74 237
Msa1437520 AT2G01630.1 40.123 324 189 3 32 351 23 345 1.83e-73 238
Msa1437520 AT3G55430.1 40.184 326 183 9 29 350 26 343 3.18e-72 233
Msa1437520 AT3G46570.1 41.515 330 182 8 26 350 21 344 1.64e-69 223
Msa1437520 AT1G66250.1 39.185 319 188 4 32 346 32 348 7.27e-68 223
Msa1437520 AT3G57270.1 38.871 319 183 8 33 348 28 337 9.58e-66 213
Msa1437520 AT5G20390.1 37.101 345 205 8 1 342 1 336 8.55e-65 211
Msa1437520 AT4G16260.1 34.870 347 213 6 8 352 1 336 5.59e-63 206
Msa1437520 AT5G20340.1 38.924 316 178 7 31 342 42 346 6.15e-63 206
Msa1437520 AT4G34480.3 47.210 233 123 0 119 351 3 235 7.52e-63 207
Msa1437520 AT3G61810.1 36.061 330 189 6 32 351 55 372 1.56e-61 203
Msa1437520 AT1G77780.1 34.483 348 215 9 17 362 8 344 2.54e-61 202
Msa1437520 AT2G39640.1 36.070 341 205 7 14 350 9 340 2.71e-61 207
Msa1437520 AT3G57260.1 36.686 338 196 10 13 348 15 336 5.36e-60 198
Msa1437520 AT3G57260.2 36.686 338 196 10 13 348 31 352 7.05e-60 198
Msa1437520 AT5G20330.1 38.291 316 180 7 31 342 33 337 2.17e-59 196
Msa1437520 AT4G16260.2 35.350 314 195 4 39 352 2 307 5.29e-59 194
Msa1437520 AT1G33220.1 37.574 338 188 9 14 338 8 335 6.69e-59 195
Msa1437520 AT5G24318.3 42.915 247 132 4 143 384 3 245 3.55e-58 192
Msa1437520 AT1G77790.1 34.493 345 213 8 7 348 7 341 1.11e-57 192
Msa1437520 AT3G57240.1 34.579 321 195 8 29 348 32 338 1.48e-52 179
Msa1437520 AT4G31140.1 31.180 356 228 11 4 352 3 348 1.38e-47 169
Msa1437520 AT3G24330.1 32.222 360 222 14 4 352 14 362 2.06e-47 169
Msa1437520 AT5G20560.1 31.642 335 211 9 11 340 15 336 3.57e-46 162
Msa1437520 AT5G64790.1 33.230 322 198 11 29 341 25 338 3.96e-45 162
Msa1437520 AT5G18220.1 32.229 332 212 8 27 352 25 349 3.08e-44 160
Msa1437520 AT1G64760.1 32.249 338 210 11 29 356 22 350 3.31e-42 154
Msa1437520 AT1G64760.2 32.249 338 210 11 29 356 22 350 3.31e-42 154
Msa1437520 AT2G19440.2 31.897 348 220 11 14 352 3 342 5.20e-42 154
Msa1437520 AT2G19440.1 31.897 348 220 11 14 352 3 342 5.20e-42 154
Msa1437520 AT4G17180.1 30.514 331 210 11 30 351 23 342 1.42e-41 152
Msa1437520 AT5G58090.1 30.422 332 218 9 28 352 18 343 1.56e-41 152
Msa1437520 AT5G20870.1 31.956 363 220 14 5 351 6 357 1.71e-41 153
Msa1437520 AT5G58480.1 31.498 327 209 9 29 347 23 342 3.00e-41 152
Msa1437520 AT3G04010.1 31.965 341 213 11 9 341 14 343 5.42e-41 151
Msa1437520 AT3G55780.1 32.075 318 200 10 46 355 49 358 9.98e-41 148

Find 66 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGAACAAGATATTGAATT+AGG 0.153002 tig0028330:-3224 None:intergenic
ACATTTGCCATTGCTAAATT+AGG 0.171344 tig0028330:+3582 Msa1437520:CDS
TATGATTCCAATCTCATTCT+AGG 0.189809 tig0028330:-3753 None:intergenic
TCATAATGTAAATTTGTATT+AGG 0.242481 tig0028330:-3528 None:intergenic
ATATTGATTTAGAGGGAAAT+AGG 0.284181 tig0028330:-2678 None:intergenic
TTCGGACGTGTTGCGTCATT+TGG 0.288335 tig0028330:+2904 Msa1437520:CDS
CTAAATAACTTGTATATTGT+TGG 0.296342 tig0028330:-3993 None:intergenic
TAGTCTAGAGATATTTGATT+AGG 0.302492 tig0028330:-3471 None:intergenic
TACAATGCTCTAGTTCAATT+AGG 0.318143 tig0028330:+3261 Msa1437520:CDS
TGTGACAAATATTGATTTAG+AGG 0.321820 tig0028330:-2686 None:intergenic
GCGTCATTTGGAATAAACTA+TGG 0.327515 tig0028330:+2916 Msa1437520:CDS
TGCTCTAGTTCAATTAGGTT+TGG 0.329748 tig0028330:+3266 Msa1437520:CDS
CTTGTTGTGGATCATTTAAT+TGG 0.341370 tig0028330:-3101 None:intergenic
ATCTTTGTGTCTGGAAGATA+TGG 0.358675 tig0028330:-3153 None:intergenic
GAGGTTAGGGTTTCTGAGAC+TGG 0.362945 tig0028330:+3618 Msa1437520:CDS
AGTCTAGAGATATTTGATTA+GGG 0.367834 tig0028330:-3470 None:intergenic
GTAAGAGAAATTGAAGTAGA+AGG 0.368771 tig0028330:-3903 None:intergenic
GATGATCTCCACGTCTGAAT+TGG 0.369675 tig0028330:-3052 None:intergenic
TTTGAATCCTAGAATGAGAT+TGG 0.371338 tig0028330:+3746 Msa1437520:CDS
AAAGGTGACTCTGATGAAAT+TGG 0.380257 tig0028330:+3651 Msa1437520:CDS
GGATCAACCATTCCTTGGTT+AGG 0.386797 tig0028330:-3507 None:intergenic
TCATTCTTATAAGCAAAATA+AGG 0.388117 tig0028330:-3447 None:intergenic
TTTAATGAAAATATGAAGAA+TGG 0.404108 tig0028330:+3789 Msa1437520:CDS
TGGAACAAGATATTGAATTA+GGG 0.410253 tig0028330:-3223 None:intergenic
TGGAAGATATGGTTTGATAT+TGG 0.415025 tig0028330:-3142 None:intergenic
CCAACCTCAGAAAGGAACTA+TGG 0.427631 tig0028330:+3813 Msa1437520:CDS
CCATAGTTCCTTTCTGAGGT+TGG 0.446684 tig0028330:-3813 None:intergenic
TATTAGGATCAACCATTCCT+TGG 0.446836 tig0028330:-3512 None:intergenic
ATCCACAACAAGCACTTCAA+TGG 0.448948 tig0028330:+3112 Msa1437520:CDS
TGATAACATGCTTTATGCTA+TGG 0.465658 tig0028330:+3548 Msa1437520:CDS
AGGATATGCATTGACCCAAA+AGG 0.470969 tig0028330:-3427 None:intergenic
TTGTAAGTCATACTTTCATC+AGG 0.472826 tig0028330:-3846 None:intergenic
AAAGTATGACTTACAATGTT+GGG 0.473433 tig0028330:+3853 Msa1437520:CDS
ATTCCAGTGATCTTTGTGTC+TGG 0.474898 tig0028330:-3162 None:intergenic
AAGATCACTGGAATTCAAGT+AGG 0.477367 tig0028330:+3171 Msa1437520:CDS
GGCAACATTTACTCAAAGAA+TGG 0.488334 tig0028330:+2711 Msa1437520:CDS
GGATATGCATTGACCCAAAA+GGG 0.491865 tig0028330:-3426 None:intergenic
TTGTAAATGTTGATCACTGC+TGG 0.497136 tig0028330:-3243 None:intergenic
TTTATAACAAGAATTTATTG+AGG 0.499075 tig0028330:+3697 Msa1437520:CDS
TTAGGGTTTCTGAGACTGGT+TGG 0.504743 tig0028330:+3622 Msa1437520:CDS
CTCTTACCTCTTCTGCCACT+AGG 0.508681 tig0028330:+3919 Msa1437520:CDS
GTGACAAATATTGATTTAGA+GGG 0.511053 tig0028330:-2685 None:intergenic
TCAAGAACTTCTAATGAACT+AGG 0.514280 tig0028330:-3312 None:intergenic
AATCTCATTCTAGGATTCAA+AGG 0.515011 tig0028330:-3744 None:intergenic
AGTTTCAGAAGTGAAATCTC+TGG 0.515867 tig0028330:+3360 Msa1437520:CDS
GAATCTTACCCTCCTTCAGC+TGG 0.518663 tig0028330:+3336 Msa1437520:CDS
GAGACTGGTTGGCCATCAAA+AGG 0.519791 tig0028330:+3633 Msa1437520:CDS
TCCACAACAAGCACTTCAAT+GGG 0.523957 tig0028330:+3113 Msa1437520:CDS
ATTTAATCCTAACCAAGGAA+TGG 0.526689 tig0028330:+3500 Msa1437520:CDS
AGGAGACAAATGAAGAATGA+AGG 0.534907 tig0028330:+3717 Msa1437520:CDS
CTTTGTCCTAGTGGCAGAAG+AGG 0.537253 tig0028330:-3925 None:intergenic
CTTCCAGACACAAAGATCAC+TGG 0.544255 tig0028330:+3159 Msa1437520:CDS
CTCTTCTGCCACTAGGACAA+AGG 0.544384 tig0028330:+3926 Msa1437520:CDS
ACCCATTGAAGTGCTTGTTG+TGG 0.545635 tig0028330:-3114 None:intergenic
GCGAAAGCTTTAAGAATCTG+TGG 0.549065 tig0028330:-3030 None:intergenic
ATTAAAACCTAATTTAGCAA+TGG 0.564223 tig0028330:-3589 None:intergenic
TAAATCAATATTTGTCACAA+TGG 0.569290 tig0028330:+2690 Msa1437520:exon
CAAACCATAGTTCCTTTCTG+AGG 0.570996 tig0028330:-3817 None:intergenic
GAAAGTATGACTTACAATGT+TGG 0.572069 tig0028330:+3852 Msa1437520:CDS
AGATCACTGGAATTCAAGTA+GGG 0.581675 tig0028330:+3172 Msa1437520:CDS
CTGAAACTACCAGCTGAAGG+AGG 0.582451 tig0028330:-3345 None:intergenic
AGAATGGACCAACCTCAGAA+AGG 0.610841 tig0028330:+3805 Msa1437520:CDS
AGCTTTCGCCAATTCAGACG+TGG 0.617283 tig0028330:+3044 Msa1437520:CDS
GTATTATTTAATCCTAACCA+AGG 0.619362 tig0028330:+3495 Msa1437520:CDS
TGAAACTACCAGCTGAAGGA+GGG 0.628259 tig0028330:-3344 None:intergenic
CTTCTGAAACTACCAGCTGA+AGG 0.634495 tig0028330:-3348 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATAATGTAAATTTGTATT+AGG - tig0028330:3531-3550 None:intergenic 15.0%
!! TTTATAACAAGAATTTATTG+AGG + tig0028330:3697-3716 Msa1437520:CDS 15.0%
!! TTTAATGAAAATATGAAGAA+TGG + tig0028330:3789-3808 Msa1437520:CDS 15.0%
!! TCATAATGTAAATTTGTATT+AGG - tig0028330:3531-3550 None:intergenic 15.0%
!! TTTATAACAAGAATTTATTG+AGG + tig0028330:3697-3716 Msa1437520:CDS 15.0%
!! TTTAATGAAAATATGAAGAA+TGG + tig0028330:3789-3808 Msa1437520:CDS 15.0%
!! TAAATCAATATTTGTCACAA+TGG + tig0028330:2690-2709 Msa1437520:exon 20.0%
!!! ATTACAGATACAGAAATTTT+CGG + tig0028330:2886-2905 Msa1437520:intron 20.0%
!! TCATTCTTATAAGCAAAATA+AGG - tig0028330:3450-3469 None:intergenic 20.0%
!! ATTAAAACCTAATTTAGCAA+TGG - tig0028330:3592-3611 None:intergenic 20.0%
!!! ATTGCTAAATTAGGTTTTAA+TGG + tig0028330:3591-3610 Msa1437520:CDS 20.0%
!!! TTGCTAAATTAGGTTTTAAT+GGG + tig0028330:3592-3611 Msa1437520:CDS 20.0%
!!! CTAAATAACTTGTATATTGT+TGG - tig0028330:3996-4015 None:intergenic 20.0%
!! TAAATCAATATTTGTCACAA+TGG + tig0028330:2690-2709 Msa1437520:exon 20.0%
!!! ATTACAGATACAGAAATTTT+CGG + tig0028330:2886-2905 Msa1437520:intron 20.0%
!! TCATTCTTATAAGCAAAATA+AGG - tig0028330:3450-3469 None:intergenic 20.0%
!! ATTAAAACCTAATTTAGCAA+TGG - tig0028330:3592-3611 None:intergenic 20.0%
!!! ATTGCTAAATTAGGTTTTAA+TGG + tig0028330:3591-3610 Msa1437520:CDS 20.0%
!!! TTGCTAAATTAGGTTTTAAT+GGG + tig0028330:3592-3611 Msa1437520:CDS 20.0%
!!! CTAAATAACTTGTATATTGT+TGG - tig0028330:3996-4015 None:intergenic 20.0%
!!! ATATTGATTTAGAGGGAAAT+AGG - tig0028330:2681-2700 None:intergenic 25.0%
!!! GTGACAAATATTGATTTAGA+GGG - tig0028330:2688-2707 None:intergenic 25.0%
!!! TGTGACAAATATTGATTTAG+AGG - tig0028330:2689-2708 None:intergenic 25.0%
! TGCATGATGAAAAGAATAAT+AGG - tig0028330:2801-2820 None:intergenic 25.0%
!!! TCTTGTTTTTGTGATCTTTA+AGG - tig0028330:2995-3014 None:intergenic 25.0%
! TGGAACAAGATATTGAATTA+GGG - tig0028330:3226-3245 None:intergenic 25.0%
!! AAAAACAAAATCACCCTTTT+GGG + tig0028330:3413-3432 Msa1437520:CDS 25.0%
! AGTCTAGAGATATTTGATTA+GGG - tig0028330:3473-3492 None:intergenic 25.0%
! TAGTCTAGAGATATTTGATT+AGG - tig0028330:3474-3493 None:intergenic 25.0%
! GTATTATTTAATCCTAACCA+AGG + tig0028330:3495-3514 Msa1437520:CDS 25.0%
! AAAGTATGACTTACAATGTT+GGG + tig0028330:3853-3872 Msa1437520:CDS 25.0%
!!! ATATTGATTTAGAGGGAAAT+AGG - tig0028330:2681-2700 None:intergenic 25.0%
!!! GTGACAAATATTGATTTAGA+GGG - tig0028330:2688-2707 None:intergenic 25.0%
!!! TGTGACAAATATTGATTTAG+AGG - tig0028330:2689-2708 None:intergenic 25.0%
! TGCATGATGAAAAGAATAAT+AGG - tig0028330:2801-2820 None:intergenic 25.0%
!!! TCTTGTTTTTGTGATCTTTA+AGG - tig0028330:2995-3014 None:intergenic 25.0%
! TGGAACAAGATATTGAATTA+GGG - tig0028330:3226-3245 None:intergenic 25.0%
!! AAAAACAAAATCACCCTTTT+GGG + tig0028330:3413-3432 Msa1437520:CDS 25.0%
! AGTCTAGAGATATTTGATTA+GGG - tig0028330:3473-3492 None:intergenic 25.0%
! TAGTCTAGAGATATTTGATT+AGG - tig0028330:3474-3493 None:intergenic 25.0%
! GTATTATTTAATCCTAACCA+AGG + tig0028330:3495-3514 Msa1437520:CDS 25.0%
! AAAGTATGACTTACAATGTT+GGG + tig0028330:3853-3872 Msa1437520:CDS 25.0%
GTGAAAAAACAGAACAAACA+GGG - tig0028330:2779-2798 None:intergenic 30.0%
! CTTGTTGTGGATCATTTAAT+TGG - tig0028330:3104-3123 None:intergenic 30.0%
TGGAAGATATGGTTTGATAT+TGG - tig0028330:3145-3164 None:intergenic 30.0%
CTGGAACAAGATATTGAATT+AGG - tig0028330:3227-3246 None:intergenic 30.0%
! TACAATGCTCTAGTTCAATT+AGG + tig0028330:3261-3280 Msa1437520:CDS 30.0%
TCAAGAACTTCTAATGAACT+AGG - tig0028330:3315-3334 None:intergenic 30.0%
! CAAAAACAAAATCACCCTTT+TGG + tig0028330:3412-3431 Msa1437520:CDS 30.0%
ATTTAATCCTAACCAAGGAA+TGG + tig0028330:3500-3519 Msa1437520:CDS 30.0%
TGATAACATGCTTTATGCTA+TGG + tig0028330:3548-3567 Msa1437520:CDS 30.0%
ACATTTGCCATTGCTAAATT+AGG + tig0028330:3582-3601 Msa1437520:CDS 30.0%
!! ATTAGGTTTTAATGGGATAG+AGG + tig0028330:3599-3618 Msa1437520:CDS 30.0%
! TTTTAATGGGATAGAGGTTA+GGG + tig0028330:3605-3624 Msa1437520:CDS 30.0%
!! AATCTCATTCTAGGATTCAA+AGG - tig0028330:3747-3766 None:intergenic 30.0%
TTTGAATCCTAGAATGAGAT+TGG + tig0028330:3746-3765 Msa1437520:CDS 30.0%
TATGATTCCAATCTCATTCT+AGG - tig0028330:3756-3775 None:intergenic 30.0%
TTGTAAGTCATACTTTCATC+AGG - tig0028330:3849-3868 None:intergenic 30.0%
! GAAAGTATGACTTACAATGT+TGG + tig0028330:3852-3871 Msa1437520:CDS 30.0%
GTAAGAGAAATTGAAGTAGA+AGG - tig0028330:3906-3925 None:intergenic 30.0%
!!! TTTTTTTACCTTTGTCCTAG+TGG - tig0028330:3937-3956 None:intergenic 30.0%
GTGAAAAAACAGAACAAACA+GGG - tig0028330:2779-2798 None:intergenic 30.0%
! CTTGTTGTGGATCATTTAAT+TGG - tig0028330:3104-3123 None:intergenic 30.0%
TGGAAGATATGGTTTGATAT+TGG - tig0028330:3145-3164 None:intergenic 30.0%
CTGGAACAAGATATTGAATT+AGG - tig0028330:3227-3246 None:intergenic 30.0%
! TACAATGCTCTAGTTCAATT+AGG + tig0028330:3261-3280 Msa1437520:CDS 30.0%
TCAAGAACTTCTAATGAACT+AGG - tig0028330:3315-3334 None:intergenic 30.0%
! CAAAAACAAAATCACCCTTT+TGG + tig0028330:3412-3431 Msa1437520:CDS 30.0%
ATTTAATCCTAACCAAGGAA+TGG + tig0028330:3500-3519 Msa1437520:CDS 30.0%
TGATAACATGCTTTATGCTA+TGG + tig0028330:3548-3567 Msa1437520:CDS 30.0%
ACATTTGCCATTGCTAAATT+AGG + tig0028330:3582-3601 Msa1437520:CDS 30.0%
!! ATTAGGTTTTAATGGGATAG+AGG + tig0028330:3599-3618 Msa1437520:CDS 30.0%
! TTTTAATGGGATAGAGGTTA+GGG + tig0028330:3605-3624 Msa1437520:CDS 30.0%
!! AATCTCATTCTAGGATTCAA+AGG - tig0028330:3747-3766 None:intergenic 30.0%
TTTGAATCCTAGAATGAGAT+TGG + tig0028330:3746-3765 Msa1437520:CDS 30.0%
TATGATTCCAATCTCATTCT+AGG - tig0028330:3756-3775 None:intergenic 30.0%
TTGTAAGTCATACTTTCATC+AGG - tig0028330:3849-3868 None:intergenic 30.0%
! GAAAGTATGACTTACAATGT+TGG + tig0028330:3852-3871 Msa1437520:CDS 30.0%
GTAAGAGAAATTGAAGTAGA+AGG - tig0028330:3906-3925 None:intergenic 30.0%
!!! TTTTTTTACCTTTGTCCTAG+TGG - tig0028330:3937-3956 None:intergenic 30.0%
GGCAACATTTACTCAAAGAA+TGG + tig0028330:2711-2730 Msa1437520:CDS 35.0%
GGTGAAAAAACAGAACAAAC+AGG - tig0028330:2780-2799 None:intergenic 35.0%
! GCGTCATTTGGAATAAACTA+TGG + tig0028330:2916-2935 Msa1437520:CDS 35.0%
!! CACAACCAGAAAAAGTACTA+GGG + tig0028330:2959-2978 Msa1437520:CDS 35.0%
ATCTTTGTGTCTGGAAGATA+TGG - tig0028330:3156-3175 None:intergenic 35.0%
AAGATCACTGGAATTCAAGT+AGG + tig0028330:3171-3190 Msa1437520:CDS 35.0%
AGATCACTGGAATTCAAGTA+GGG + tig0028330:3172-3191 Msa1437520:CDS 35.0%
TTGTAAATGTTGATCACTGC+TGG - tig0028330:3246-3265 None:intergenic 35.0%
! TGCTCTAGTTCAATTAGGTT+TGG + tig0028330:3266-3285 Msa1437520:CDS 35.0%
AGTTTCAGAAGTGAAATCTC+TGG + tig0028330:3360-3379 Msa1437520:CDS 35.0%
TATTAGGATCAACCATTCCT+TGG - tig0028330:3515-3534 None:intergenic 35.0%
!! GTTTTAATGGGATAGAGGTT+AGG + tig0028330:3604-3623 Msa1437520:CDS 35.0%
AAAGGTGACTCTGATGAAAT+TGG + tig0028330:3651-3670 Msa1437520:CDS 35.0%
AGGAGACAAATGAAGAATGA+AGG + tig0028330:3717-3736 Msa1437520:CDS 35.0%
GGCAACATTTACTCAAAGAA+TGG + tig0028330:2711-2730 Msa1437520:CDS 35.0%
GGTGAAAAAACAGAACAAAC+AGG - tig0028330:2780-2799 None:intergenic 35.0%
! GCGTCATTTGGAATAAACTA+TGG + tig0028330:2916-2935 Msa1437520:CDS 35.0%
!! CACAACCAGAAAAAGTACTA+GGG + tig0028330:2959-2978 Msa1437520:CDS 35.0%
ATCTTTGTGTCTGGAAGATA+TGG - tig0028330:3156-3175 None:intergenic 35.0%
AAGATCACTGGAATTCAAGT+AGG + tig0028330:3171-3190 Msa1437520:CDS 35.0%
AGATCACTGGAATTCAAGTA+GGG + tig0028330:3172-3191 Msa1437520:CDS 35.0%
TTGTAAATGTTGATCACTGC+TGG - tig0028330:3246-3265 None:intergenic 35.0%
! TGCTCTAGTTCAATTAGGTT+TGG + tig0028330:3266-3285 Msa1437520:CDS 35.0%
AGTTTCAGAAGTGAAATCTC+TGG + tig0028330:3360-3379 Msa1437520:CDS 35.0%
TATTAGGATCAACCATTCCT+TGG - tig0028330:3515-3534 None:intergenic 35.0%
!! GTTTTAATGGGATAGAGGTT+AGG + tig0028330:3604-3623 Msa1437520:CDS 35.0%
AAAGGTGACTCTGATGAAAT+TGG + tig0028330:3651-3670 Msa1437520:CDS 35.0%
AGGAGACAAATGAAGAATGA+AGG + tig0028330:3717-3736 Msa1437520:CDS 35.0%
!!! CTAGCATTTTTTCTCTCTGC+AGG + tig0028330:2751-2770 Msa1437520:CDS 40.0%
!!! CCTAGTACTTTTTCTGGTTG+TGG - tig0028330:2961-2980 None:intergenic 40.0%
!! CCACAACCAGAAAAAGTACT+AGG + tig0028330:2958-2977 Msa1437520:CDS 40.0%
!!! AGAAGCCCTAGTACTTTTTC+TGG - tig0028330:2967-2986 None:intergenic 40.0%
!! GCGAAAGCTTTAAGAATCTG+TGG - tig0028330:3033-3052 None:intergenic 40.0%
ATCCACAACAAGCACTTCAA+TGG + tig0028330:3112-3131 Msa1437520:CDS 40.0%
TCCACAACAAGCACTTCAAT+GGG + tig0028330:3113-3132 Msa1437520:CDS 40.0%
ATTCCAGTGATCTTTGTGTC+TGG - tig0028330:3165-3184 None:intergenic 40.0%
! GGATATGCATTGACCCAAAA+GGG - tig0028330:3429-3448 None:intergenic 40.0%
! AGGATATGCATTGACCCAAA+AGG - tig0028330:3430-3449 None:intergenic 40.0%
! TCATCAGAGTCACCTTTTGA+TGG - tig0028330:3648-3667 None:intergenic 40.0%
CAAACCATAGTTCCTTTCTG+AGG - tig0028330:3820-3839 None:intergenic 40.0%
!!! CTAGCATTTTTTCTCTCTGC+AGG + tig0028330:2751-2770 Msa1437520:CDS 40.0%
!!! CCTAGTACTTTTTCTGGTTG+TGG - tig0028330:2961-2980 None:intergenic 40.0%
!! CCACAACCAGAAAAAGTACT+AGG + tig0028330:2958-2977 Msa1437520:CDS 40.0%
!!! AGAAGCCCTAGTACTTTTTC+TGG - tig0028330:2967-2986 None:intergenic 40.0%
!! GCGAAAGCTTTAAGAATCTG+TGG - tig0028330:3033-3052 None:intergenic 40.0%
ATCCACAACAAGCACTTCAA+TGG + tig0028330:3112-3131 Msa1437520:CDS 40.0%
TCCACAACAAGCACTTCAAT+GGG + tig0028330:3113-3132 Msa1437520:CDS 40.0%
ATTCCAGTGATCTTTGTGTC+TGG - tig0028330:3165-3184 None:intergenic 40.0%
! GGATATGCATTGACCCAAAA+GGG - tig0028330:3429-3448 None:intergenic 40.0%
! AGGATATGCATTGACCCAAA+AGG - tig0028330:3430-3449 None:intergenic 40.0%
! TCATCAGAGTCACCTTTTGA+TGG - tig0028330:3648-3667 None:intergenic 40.0%
CAAACCATAGTTCCTTTCTG+AGG - tig0028330:3820-3839 None:intergenic 40.0%
GATGATCTCCACGTCTGAAT+TGG - tig0028330:3055-3074 None:intergenic 45.0%
! ACCCATTGAAGTGCTTGTTG+TGG - tig0028330:3117-3136 None:intergenic 45.0%
CTTCCAGACACAAAGATCAC+TGG + tig0028330:3159-3178 Msa1437520:CDS 45.0%
TGAAACTACCAGCTGAAGGA+GGG - tig0028330:3347-3366 None:intergenic 45.0%
!! CTTCTGAAACTACCAGCTGA+AGG - tig0028330:3351-3370 None:intergenic 45.0%
GGATCAACCATTCCTTGGTT+AGG - tig0028330:3510-3529 None:intergenic 45.0%
!! TTAGGGTTTCTGAGACTGGT+TGG + tig0028330:3622-3641 Msa1437520:CDS 45.0%
AGAATGGACCAACCTCAGAA+AGG + tig0028330:3805-3824 Msa1437520:CDS 45.0%
!! CCATAGTTCCTTTCTGAGGT+TGG - tig0028330:3816-3835 None:intergenic 45.0%
CCAACCTCAGAAAGGAACTA+TGG + tig0028330:3813-3832 Msa1437520:CDS 45.0%
GATGATCTCCACGTCTGAAT+TGG - tig0028330:3055-3074 None:intergenic 45.0%
! ACCCATTGAAGTGCTTGTTG+TGG - tig0028330:3117-3136 None:intergenic 45.0%
CTTCCAGACACAAAGATCAC+TGG + tig0028330:3159-3178 Msa1437520:CDS 45.0%
TGAAACTACCAGCTGAAGGA+GGG - tig0028330:3347-3366 None:intergenic 45.0%
!! CTTCTGAAACTACCAGCTGA+AGG - tig0028330:3351-3370 None:intergenic 45.0%
GGATCAACCATTCCTTGGTT+AGG - tig0028330:3510-3529 None:intergenic 45.0%
!! TTAGGGTTTCTGAGACTGGT+TGG + tig0028330:3622-3641 Msa1437520:CDS 45.0%
AGAATGGACCAACCTCAGAA+AGG + tig0028330:3805-3824 Msa1437520:CDS 45.0%
!! CCATAGTTCCTTTCTGAGGT+TGG - tig0028330:3816-3835 None:intergenic 45.0%
CCAACCTCAGAAAGGAACTA+TGG + tig0028330:3813-3832 Msa1437520:CDS 45.0%
TTCGGACGTGTTGCGTCATT+TGG + tig0028330:2904-2923 Msa1437520:CDS 50.0%
AGCTTTCGCCAATTCAGACG+TGG + tig0028330:3044-3063 Msa1437520:CDS 50.0%
GAATCTTACCCTCCTTCAGC+TGG + tig0028330:3336-3355 Msa1437520:CDS 50.0%
CTGAAACTACCAGCTGAAGG+AGG - tig0028330:3348-3367 None:intergenic 50.0%
!! GAGGTTAGGGTTTCTGAGAC+TGG + tig0028330:3618-3637 Msa1437520:CDS 50.0%
! GAGACTGGTTGGCCATCAAA+AGG + tig0028330:3633-3652 Msa1437520:CDS 50.0%
CTCTTACCTCTTCTGCCACT+AGG + tig0028330:3919-3938 Msa1437520:CDS 50.0%
CTTTGTCCTAGTGGCAGAAG+AGG - tig0028330:3928-3947 None:intergenic 50.0%
CTCTTCTGCCACTAGGACAA+AGG + tig0028330:3926-3945 Msa1437520:CDS 50.0%
TTCGGACGTGTTGCGTCATT+TGG + tig0028330:2904-2923 Msa1437520:CDS 50.0%
AGCTTTCGCCAATTCAGACG+TGG + tig0028330:3044-3063 Msa1437520:CDS 50.0%
GAATCTTACCCTCCTTCAGC+TGG + tig0028330:3336-3355 Msa1437520:CDS 50.0%
CTGAAACTACCAGCTGAAGG+AGG - tig0028330:3348-3367 None:intergenic 50.0%
!! GAGGTTAGGGTTTCTGAGAC+TGG + tig0028330:3618-3637 Msa1437520:CDS 50.0%
! GAGACTGGTTGGCCATCAAA+AGG + tig0028330:3633-3652 Msa1437520:CDS 50.0%
CTCTTACCTCTTCTGCCACT+AGG + tig0028330:3919-3938 Msa1437520:CDS 50.0%
CTTTGTCCTAGTGGCAGAAG+AGG - tig0028330:3928-3947 None:intergenic 50.0%
CTCTTCTGCCACTAGGACAA+AGG + tig0028330:3926-3945 Msa1437520:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0028330 gene 2655 4022 2655 ID=Msa1437520;Name=Msa1437520
tig0028330 mRNA 2655 4022 2655 ID=Msa1437520-mRNA-1;Parent=Msa1437520;Name=Msa1437520-mRNA-1;_AED=0.09;_eAED=0.09;_QI=54|1|0.5|1|1|1|2|0|397
tig0028330 exon 2655 2772 2655 ID=Msa1437520-mRNA-1:exon:10733;Parent=Msa1437520-mRNA-1
tig0028330 exon 2893 4022 2893 ID=Msa1437520-mRNA-1:exon:10734;Parent=Msa1437520-mRNA-1
tig0028330 five_prime_UTR 2655 2708 2655 ID=Msa1437520-mRNA-1:five_prime_utr;Parent=Msa1437520-mRNA-1
tig0028330 CDS 2709 2772 2709 ID=Msa1437520-mRNA-1:cds;Parent=Msa1437520-mRNA-1
tig0028330 CDS 2893 4022 2893 ID=Msa1437520-mRNA-1:cds;Parent=Msa1437520-mRNA-1
Gene Sequence

>Msa1437520

ATGGCAACATTTACTCAAAGAATGGCAATATGTTATATTCTTCTAGCATTTTTTCTCTCTGCAGATACAGAAATTTTCGGACGTGTTGCGTCATTTGGAATAAACTATGGACAAGTAGCTAACAATTTGCCACAACCAGAAAAAGTACTAGGGCTTCTCAGCACCTTAAAGATCACAAAAACAAGAATCTACGATACGAATCCACAGATTCTTAAAGCTTTCGCCAATTCAGACGTGGAGATCATCGTAACAATTGAAAATCAAATACTAAACCAATTAAATGATCCACAACAAGCACTTCAATGGGTAAATACCAATATCAAACCATATCTTCCAGACACAAAGATCACTGGAATTCAAGTAGGGAATGAAGTGTTCACAGAACTAGACACAACCCTAATTCAATATCTTGTTCCAGCAGTGATCAACATTTACAATGCTCTAGTTCAATTAGGTTTGGACTCAAACATTCACGTGTCAACACCTAGTTCATTAGAAGTTCTTGAAGAATCTTACCCTCCTTCAGCTGGTAGTTTCAGAAGTGAAATCTCTGGAATCATGCATGATTTTTTGAACTTTTTGTCAAAAACAAAATCACCCTTTTGGGTCAATGCATATCCTTATTTTGCTTATAAGAATGACCCTAATCAAATATCTCTAGACTATGTATTATTTAATCCTAACCAAGGAATGGTTGATCCTAATACAAATTTACATTATGATAACATGCTTTATGCTATGGTAGATGCTGTTACATTTGCCATTGCTAAATTAGGTTTTAATGGGATAGAGGTTAGGGTTTCTGAGACTGGTTGGCCATCAAAAGGTGACTCTGATGAAATTGGTGCATCATTGAAAAATGCTGAAATTTATAACAAGAATTTATTGAGGAGACAAATGAAGAATGAAGGAACACCTTTGAATCCTAGAATGAGATTGGAATCATATTTGTTTGCATTATTTAATGAAAATATGAAGAATGGACCAACCTCAGAAAGGAACTATGGTTTGTTTGAGCCTGATGAAAGTATGACTTACAATGTTGGGTTGTCTTCTTTTGCAAAGTCATCAAATCCTTCTACTTCAATTTCTCTTACCTCTTCTGCCACTAGGACAAAGGTAAAAAAAAAAAAGAGTAAAGACATTGTTTTTTTTCTTTCTTTTCCAACAATATACAAGTTATTTAGCTTATAG
Protein sequence

>Msa1437520

MATFTQRMAICYILLAFFLSADTEIFGRVASFGINYGQVANNLPQPEKVLGLLSTLKITKTRIYDTNPQILKAFANSDVEIIVTIENQILNQLNDPQQALQWVNTNIKPYLPDTKITGIQVGNEVFTELDTTLIQYLVPAVINIYNALVQLGLDSNIHVSTPSSLEVLEESYPPSAGSFRSEISGIMHDFLNFLSKTKSPFWVNAYPYFAYKNDPNQISLDYVLFNPNQGMVDPNTNLHYDNMLYAMVDAVTFAIAKLGFNGIEVRVSETGWPSKGDSDEIGASLKNAEIYNKNLLRRQMKNEGTPLNPRMRLESYLFALFNENMKNGPTSERNYGLFEPDESMTYNVGLSSFAKSSNPSTSISLTSSATRTKVKKKKSKDIVFFLSFPTIYKLFSL*