AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0660890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660890 MtrunA17_Chr5g0404341 98.077 572 5 1 1 572 1 566 0.0 1160
Msa0660890 MtrunA17_Chr5g0404361 82.250 569 92 4 5 572 51 611 0.0 981
Msa0660890 MtrunA17_Chr5g0429511 60.215 558 215 2 15 572 8 558 0.0 723
Msa0660890 MtrunA17_Chr3g0104061 62.500 544 196 3 29 572 21 556 0.0 722
Msa0660890 MtrunA17_Chr4g0005921 60.213 563 215 3 12 572 2 557 0.0 696
Msa0660890 MtrunA17_Chr5g0436461 56.014 557 229 4 21 572 9 554 0.0 673
Msa0660890 MtrunA17_Chr7g0238981 57.989 557 212 8 31 572 44 593 0.0 663
Msa0660890 MtrunA17_Chr7g0237491 54.188 585 243 8 6 572 3 580 0.0 649
Msa0660890 MtrunA17_Chr7g0237441 54.592 588 243 9 1 572 1 580 0.0 648
Msa0660890 MtrunA17_Chr7g0238351 55.102 588 236 9 6 572 1 581 0.0 648
Msa0660890 MtrunA17_Chr7g0238941 53.082 584 252 9 4 572 2 578 0.0 637
Msa0660890 MtrunA17_Chr4g0031901 53.235 541 234 6 31 560 41 573 0.0 607
Msa0660890 MtrunA17_Chr5g0437681 48.973 584 265 8 5 572 1 567 0.0 587
Msa0660890 MtrunA17_Chr3g0114211 49.486 584 265 9 5 572 1 570 0.0 580
Msa0660890 MtrunA17_Chr5g0431581 51.399 572 252 9 13 572 3 560 0.0 577
Msa0660890 MtrunA17_Chr2g0278811 44.658 571 285 6 33 572 37 607 6.24e-177 513
Msa0660890 MtrunA17_Chr4g0008521 44.784 556 283 6 33 572 25 572 5.25e-174 504
Msa0660890 MtrunA17_Chr8g0346581 42.295 584 312 8 1 572 1 571 2.28e-170 495
Msa0660890 MtrunA17_Chr7g0240991 44.014 568 292 11 22 572 13 571 1.09e-163 478
Msa0660890 MtrunA17_Chr7g0240971 44.077 574 296 8 14 572 7 570 2.46e-156 459
Msa0660890 MtrunA17_Chr3g0133861 46.769 325 160 9 242 555 1 323 6.89e-86 270
Msa0660890 MtrunA17_Chr8g0382691 33.942 548 318 16 29 555 29 553 9.62e-83 269
Msa0660890 MtrunA17_Chr5g0436431 52.155 232 104 2 302 530 1 228 8.22e-80 250
Msa0660890 MtrunA17_Chr3g0118321 31.818 594 340 22 12 572 1 562 6.23e-70 235
Msa0660890 MtrunA17_Chr3g0118331 30.580 569 340 19 12 550 1 544 5.49e-65 222
Msa0660890 MtrunA17_Chr3g0133871 47.642 212 110 1 6 216 3 214 5.82e-63 206
Msa0660890 MtrunA17_Chr4g0038671 29.268 574 339 18 15 572 10 532 1.59e-58 204
Msa0660890 MtrunA17_Chr4g0056511 28.920 574 369 17 10 572 4 549 4.01e-58 204
Msa0660890 MtrunA17_Chr3g0143671 29.234 561 335 18 27 572 20 533 1.42e-57 201
Msa0660890 MtrunA17_Chr1g0157341 28.147 572 353 16 14 572 6 532 1.46e-54 193
Msa0660890 MtrunA17_Chr3g0079321 27.624 543 356 16 5 537 1 516 6.21e-54 192
Msa0660890 MtrunA17_Chr4g0024861 29.903 515 302 18 30 532 23 490 2.64e-53 190
Msa0660890 MtrunA17_Chr2g0322441 31.055 512 300 17 30 532 26 493 1.72e-49 179
Msa0660890 MtrunA17_Chr7g0234061 28.180 511 331 16 36 537 31 514 5.20e-49 179
Msa0660890 MtrunA17_Chr8g0374271 28.983 521 300 19 29 532 26 493 1.18e-48 177
Msa0660890 MtrunA17_Chr8g0374261 27.869 549 309 21 17 538 9 497 1.34e-47 174
Msa0660890 MtrunA17_Chr5g0402831 28.107 523 311 18 29 538 26 496 5.93e-46 169
Msa0660890 MtrunA17_Chr4g0043261 27.556 577 355 20 15 571 6 539 1.14e-44 166
Msa0660890 MtrunA17_Chr4g0075071 30.427 539 302 20 13 532 10 494 1.27e-43 163
Msa0660890 MtrunA17_Chr3g0121161 27.163 578 363 22 13 571 46 584 2.28e-43 163
Msa0660890 MtrunA17_Chr3g0121151 27.163 578 363 22 13 571 46 584 5.18e-43 162
Msa0660890 MtrunA17_Chr3g0121171 27.194 581 359 23 13 571 1156 1694 2.62e-42 163
Msa0660890 MtrunA17_Chr3g0121171 27.163 578 363 22 13 571 581 1119 5.13e-42 162
Msa0660890 MtrunA17_Chr3g0121171 27.568 555 345 21 36 571 28 544 9.76e-42 162
Msa0660890 MtrunA17_Chr3g0121171 27.652 528 325 21 63 571 1771 2260 2.64e-36 145
Msa0660890 MtrunA17_Chr4g0024641 29.630 459 261 20 88 532 3 413 1.29e-37 149
Msa0660890 MtrunA17_Chr4g0043251 27.106 546 327 23 52 571 45 545 3.04e-37 145
Msa0660890 MtrunA17_Chr3g0118351 32.416 327 178 11 253 551 6 317 4.36e-31 124
Msa0660890 MtrunA17_Chr1g0197301 27.978 361 213 14 227 572 1 329 3.47e-27 112
Msa0660890 MtrunA17_Chr3g0118341 34.483 174 106 6 12 181 4 173 3.40e-24 100
Msa0660890 MtrunA17_Chr5g0436411 46.479 71 36 1 21 89 9 79 1.33e-16 74.7
Msa0660890 MtrunA17_Chr1g0197311 28.251 223 112 8 44 231 34 243 2.21e-16 79.0
Msa0660890 MtrunA17_Chr4g0075781 29.518 166 94 5 142 302 5 152 1.14e-13 69.3
Msa0660890 MtrunA17_Chr3g0118931 38.462 91 51 2 482 572 10 95 3.50e-11 60.5
Msa0660890 MtrunA17_Chr8g0370581 82.143 28 5 0 189 216 1 28 3.95e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660890 AT5G03260.1 75.313 559 129 3 14 572 8 557 0.0 889
Msa0660890 AT2G38080.1 60.708 565 211 4 10 572 3 558 0.0 729
Msa0660890 AT5G58910.2 58.699 569 224 4 9 572 5 567 0.0 699
Msa0660890 AT5G58910.3 58.699 569 223 5 9 572 5 566 0.0 695
Msa0660890 AT5G58910.1 60.075 536 196 5 42 572 1 523 0.0 667
Msa0660890 AT5G01190.1 57.904 563 221 7 15 572 7 558 0.0 655
Msa0660890 AT5G60020.1 54.124 582 239 8 12 572 3 577 0.0 646
Msa0660890 AT2G29130.1 54.007 574 237 10 17 572 9 573 0.0 635
Msa0660890 AT5G60020.3 55.797 552 216 8 42 572 1 545 0.0 633
Msa0660890 AT5G01190.2 57.016 563 212 8 15 572 7 544 0.0 632
Msa0660890 AT5G05390.1 50.704 568 257 9 17 572 9 565 0.0 588
Msa0660890 AT5G60020.2 52.963 540 226 8 12 530 3 535 0.0 578
Msa0660890 AT1G18140.1 49.644 562 257 8 31 572 26 581 0.0 578
Msa0660890 AT2G30210.1 49.228 583 268 8 5 572 1 570 0.0 575
Msa0660890 AT2G40370.1 47.692 585 268 9 15 572 7 580 0.0 556
Msa0660890 AT5G07130.1 48.205 585 270 10 5 572 1 569 0.0 548
Msa0660890 AT5G09360.1 47.100 569 279 10 13 572 14 569 0.0 543
Msa0660890 AT3G09220.1 45.714 560 285 7 5 555 1 550 3.85e-172 500
Msa0660890 AT2G46570.1 44.704 557 281 10 29 572 27 569 6.78e-172 499
Msa0660890 AT5G07130.2 50.101 493 219 9 97 572 2 484 5.93e-166 481
Msa0660890 AT5G07130.3 50.101 493 219 9 97 572 2 484 5.93e-166 481
Msa0660890 AT5G01040.1 43.772 578 297 9 9 572 3 566 4.42e-164 480
Msa0660890 AT5G01050.1 43.523 579 299 9 9 572 3 568 5.98e-164 479
Msa0660890 AT5G48100.1 41.993 562 296 12 14 562 9 553 1.72e-152 449
Msa0660890 AT2G46570.2 44.242 495 249 10 91 572 4 484 6.46e-148 435
Msa0660890 AT3G09220.2 46.507 458 230 6 107 555 2 453 1.11e-142 421
Msa0660890 AT4G39830.1 32.215 596 337 20 12 572 14 577 1.18e-83 272
Msa0660890 AT5G21105.1 32.874 581 338 19 1 551 1 559 1.83e-79 261
Msa0660890 AT5G21100.1 31.743 545 325 14 31 551 21 542 5.82e-76 252
Msa0660890 AT5G21105.3 32.321 560 327 19 1 530 1 538 1.35e-70 237
Msa0660890 AT4G39830.2 32.742 507 275 19 100 572 8 482 7.64e-63 214
Msa0660890 AT4G39830.3 32.742 507 275 19 100 572 6 480 7.69e-63 214
Msa0660890 AT4G39830.4 32.283 508 263 18 100 572 8 469 2.30e-59 205
Msa0660890 AT1G41830.1 28.055 581 354 18 5 572 2 531 5.31e-57 200
Msa0660890 AT1G76160.1 28.219 567 349 15 17 572 11 530 1.11e-56 199
Msa0660890 AT4G12420.1 28.008 532 348 15 16 537 6 512 1.87e-56 200
Msa0660890 AT4G12420.2 28.008 532 348 15 16 537 6 512 1.87e-56 200
Msa0660890 AT4G25240.2 28.328 586 366 20 5 572 1 550 6.03e-56 198
Msa0660890 AT4G25240.1 28.328 586 366 20 5 572 1 550 6.03e-56 198
Msa0660890 AT1G21850.1 28.571 539 315 17 17 538 11 496 2.18e-55 196
Msa0660890 AT5G51480.1 27.336 578 355 20 17 572 16 550 1.41e-54 195
Msa0660890 AT1G75790.1 29.508 549 327 16 31 564 24 527 2.11e-54 193
Msa0660890 AT5G66920.1 28.699 561 327 19 30 572 33 538 3.73e-54 192
Msa0660890 AT1G21860.1 28.364 550 328 17 1 538 1 496 6.10e-54 192
Msa0660890 AT4G28090.1 27.820 532 324 15 55 572 49 534 1.31e-53 191
Msa0660890 AT4G22010.1 29.361 579 337 20 10 572 8 530 2.93e-52 187
Msa0660890 AT4G38420.1 28.327 526 319 16 22 532 17 499 2.26e-51 185
Msa0660890 AT5G48450.2 28.684 509 313 17 36 532 31 501 5.79e-50 182
Msa0660890 AT5G48450.1 28.684 509 313 17 36 532 31 501 1.79e-49 181
Msa0660890 AT4G38420.3 28.150 508 307 16 22 514 17 481 4.31e-47 172
Msa0660890 AT5G21105.2 34.234 333 198 9 225 551 51 368 1.24e-44 163
Msa0660890 AT4G38420.2 27.708 480 290 15 22 486 17 454 2.89e-43 160
Msa0660890 AT2G23630.1 29.341 501 290 19 52 538 48 498 1.10e-42 161
Msa0660890 AT2G23630.2 29.341 501 290 19 52 538 53 503 1.29e-42 160
Msa0660890 AT4G37160.1 26.617 541 330 19 14 538 11 500 1.88e-41 157
Msa0660890 AT1G55560.1 28.283 495 299 21 52 532 44 496 5.92e-38 147
Msa0660890 AT1G55570.1 26.829 492 311 16 52 532 47 500 1.77e-36 143
Msa0660890 AT3G13400.2 27.202 511 316 20 36 532 29 497 1.35e-35 140
Msa0660890 AT3G13400.1 27.202 511 316 20 36 532 29 497 1.35e-35 140
Msa0660890 AT3G13390.1 26.190 504 327 16 38 532 32 499 3.41e-34 137
Msa0660890 AT4G37160.2 26.176 489 295 18 14 486 11 449 2.77e-33 132

Find 143 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTGTTATATTTATCTTTC+TGG 0.115926 4_3:+72023000 Msa0660890:three_prime_UTR
AAACCAATTGTAACAGTAAA+TGG 0.140664 4_3:+72020381 Msa0660890:CDS
TGGTTAAGGGCTACTGTTTA+TGG 0.146954 4_3:+72020751 Msa0660890:CDS
AGACCCTGCTAAATATAATT+TGG 0.195177 4_3:+72022304 Msa0660890:CDS
GTTAAACTTGCCTAGCATTT+TGG 0.196468 4_3:-72022781 None:intergenic
CAGCTCAACCGTAGAATTAA+AGG 0.252917 4_3:-72022174 None:intergenic
TCACATCCATTTCATCTTCA+TGG 0.290780 4_3:+72022230 Msa0660890:CDS
ACAACACAATCCATTCTAAT+TGG 0.290849 4_3:+72021621 Msa0660890:CDS
ATGCTCATATTCTTTGGTTA+AGG 0.305616 4_3:+72020737 Msa0660890:CDS
GCATATAACTCACATCAAAT+TGG 0.309196 4_3:-72019809 None:intergenic
TGTTGCTACAAGAACTTTCA+TGG 0.327046 4_3:+72021701 Msa0660890:CDS
TTCTTTATTGCTGCTTCAAC+AGG 0.329252 4_3:-72019783 None:intergenic
GGAAAAGGGGTTCCTGGTTT+AGG 0.331002 4_3:-72020790 None:intergenic
GTATGTGCATCCGACATATT+TGG 0.355480 4_3:-72021180 None:intergenic
TGTTTGGAATTCCTTTGTAT+TGG 0.355508 4_3:-72021767 None:intergenic
GGTGGCATGCTCATATTCTT+TGG 0.356615 4_3:+72020731 Msa0660890:CDS
AAAGGAGCCAAAGGCTTATC+AGG 0.358204 4_3:-72022083 None:intergenic
ATTGTTATATTTATCTTTCT+GGG 0.364752 4_3:+72023001 Msa0660890:three_prime_UTR
CTCCCATTTACTGTTACAAT+TGG 0.366040 4_3:-72020384 None:intergenic
TGCTCATATTCTTTGGTTAA+GGG 0.367150 4_3:+72020738 Msa0660890:CDS
TTTGTTGTTGGAACTGGAAT+TGG 0.368677 4_3:+72022263 Msa0660890:CDS
TTTAGAGTTGCACACTGGTT+GGG 0.368974 4_3:+72022684 Msa0660890:CDS
GTCTCATAGTTCAGATTGTA+TGG 0.369346 4_3:+72022835 Msa0660890:three_prime_UTR
GTTATTGTTCTTACGTTTCT+CGG 0.370163 4_3:-72021238 None:intergenic
ATGTATAGCTTCCACCAGTT+TGG 0.371631 4_3:-72020672 None:intergenic
GGTGCGCCAGTATAGTTAAA+AGG 0.375538 4_3:-72022101 None:intergenic
GCAACCCCATTTGGTTCCCT+TGG 0.381589 4_3:-72021155 None:intergenic
AAGTTTGTGTCATTTGCATT+AGG 0.383453 4_3:-72021819 None:intergenic
ATATAATTTGGTTGATCCTA+TGG 0.386113 4_3:+72022316 Msa0660890:CDS
AGCTCATCACTTTGATATCA+AGG 0.386540 4_3:+72022040 Msa0660890:CDS
GGAAAAGGAAAAGGGGTTCC+TGG 0.388932 4_3:-72020796 None:intergenic
CTATGGAGAGGAACACTGTT+GGG 0.398198 4_3:+72022333 Msa0660890:CDS
ACGGCTTTCGTTGTGGAAGA+TGG 0.405749 4_3:+72022715 Msa0660890:CDS
TGCTTTGACTAGAACATTTG+TGG 0.409362 4_3:-72021655 None:intergenic
TCAACCAAATTATATTTAGC+AGG 0.412948 4_3:-72022308 None:intergenic
TCTCTAGCAGGTTGTGGAAA+AGG 0.413807 4_3:-72020811 None:intergenic
TTATAACCATGAAGATGAAA+TGG 0.419238 4_3:-72022236 None:intergenic
AATGAAGGGATGATAGTGTT+TGG 0.419479 4_3:-72021783 None:intergenic
CACTGGTTGGGGATTGAAAA+CGG 0.424322 4_3:+72022696 Msa0660890:CDS
CTTTAATTCTACGGTTGAGC+TGG 0.424324 4_3:+72022175 Msa0660890:CDS
TATTTGGTGGCAACCCCATT+TGG 0.424538 4_3:-72021164 None:intergenic
CCTTCTGTAGCATAAATTGT+TGG 0.426238 4_3:-72020417 None:intergenic
TTTGCATTAGGAAGTGTTGT+AGG 0.428912 4_3:-72021807 None:intergenic
TATTTAGCAGGGTCTTTAGC+AGG 0.438959 4_3:-72022296 None:intergenic
TTTATTGTGTAACAGGAGAA+TGG 0.442017 4_3:+72021103 Msa0660890:intron
TCGGATGCACATACAATCAA+TGG 0.442094 4_3:+72021189 Msa0660890:CDS
GGAAGTGTTGTAGGAAATGA+AGG 0.446604 4_3:-72021798 None:intergenic
ACTTTGAGAGGAACATTAGC+AGG 0.448431 4_3:-72021891 None:intergenic
CAAGATCGCCTTTAATTCTA+CGG 0.451231 4_3:+72022166 Msa0660890:CDS
GTAGCATAAATTGTTGGCCC+TGG 0.454242 4_3:-72020411 None:intergenic
GCCCGTCTTAGTACCGAGAT+TGG 0.456234 4_3:-72022132 None:intergenic
CTAAACCAATAGTGTAGAAA+AGG 0.460863 4_3:-72021923 None:intergenic
CGGCTTTCGTTGTGGAAGAT+GGG 0.464431 4_3:+72022716 Msa0660890:CDS
ACTCAATGTCCAATCCAAAC+TGG 0.464665 4_3:+72020658 Msa0660890:CDS
TATCGCAATGGTTGGGCTGA+TGG 0.465845 4_3:+72020622 Msa0660890:CDS
AACCAATTGTAACAGTAAAT+GGG 0.467133 4_3:+72020382 Msa0660890:CDS
GTAAACCAAGGGAACCAAAT+GGG 0.470340 4_3:+72021150 Msa0660890:CDS
TTCGTTGTGGAAGATGGGCC+AGG 0.471809 4_3:+72022721 Msa0660890:CDS
TCAAATTCTCTAGCAGGTTG+TGG 0.473611 4_3:-72020817 None:intergenic
TAGCTTCCACCAGTTTGGAT+TGG 0.474223 4_3:-72020667 None:intergenic
GCAGGTTGTGGAAAAGGAAA+AGG 0.474816 4_3:-72020805 None:intergenic
TTGACCAGGACCAATTAGAA+TGG 0.476488 4_3:-72021631 None:intergenic
TTAGAGTTGCACACTGGTTG+GGG 0.479004 4_3:+72022685 Msa0660890:CDS
CAAAACAAAAGTTGTCTAAG+AGG 0.485707 4_3:+72019673 Msa0660890:five_prime_UTR
ACAAGTTCAAATTCTCTAGC+AGG 0.487160 4_3:-72020823 None:intergenic
TTGGACCGCAATTCGATTCA+CGG 0.487234 4_3:+72022370 Msa0660890:CDS
TCTTGTCCTACATGCATCAA+TGG 0.490940 4_3:+72021954 Msa0660890:CDS
GAACAAGGAAAGAGTGGTCC+TGG 0.497425 4_3:-72021216 None:intergenic
CGATTCACGGCTAATAATCC+AGG 0.504440 4_3:+72022383 Msa0660890:CDS
AATATAACATATCAATTCAC+TGG 0.504449 4_3:+72020475 Msa0660890:CDS
TTGGGGTCCCTACCGGTGGT+TGG 0.507604 4_3:+72022351 Msa0660890:CDS
CAGGTTGTGGAAAAGGAAAA+GGG 0.509861 4_3:-72020804 None:intergenic
AACACTGTTGGGGTCCCTAC+CGG 0.517718 4_3:+72022344 Msa0660890:CDS
TTTGGAAGGTCCTTTGGTGG+TGG 0.517886 4_3:-72022763 None:intergenic
TTTCATGGATGCACCACTCT+CGG 0.522361 4_3:+72021716 Msa0660890:CDS
AGTAAACCAAGGGAACCAAA+TGG 0.525185 4_3:+72021149 Msa0660890:CDS
CAGGACAAAGAGGAACTTTG+TGG 0.526013 4_3:+72020710 Msa0660890:CDS
TTAAACAGTATCGCAATGGT+TGG 0.526070 4_3:+72020614 Msa0660890:CDS
AGAATGGATTGTGTTGTGAA+TGG 0.527678 4_3:-72021615 None:intergenic
TCGAATTGCGGTCCAACCAC+CGG 0.530904 4_3:-72022363 None:intergenic
ATTTAGAGTTGCACACTGGT+TGG 0.533023 4_3:+72022683 Msa0660890:CDS
TTCTTACGTTTCTCGGAACA+AGG 0.533666 4_3:-72021231 None:intergenic
TAAACAGTATCGCAATGGTT+GGG 0.534891 4_3:+72020615 Msa0660890:CDS
GCCAATCTCGGTACTAAGAC+GGG 0.536336 4_3:+72022131 Msa0660890:CDS
AGAACATTTGTGGTTTGACC+AGG 0.539222 4_3:-72021645 None:intergenic
CGGATGCACATACAATCAAT+GGG 0.539224 4_3:+72021190 Msa0660890:CDS
AGAGAATTTGAACTTGTGCT+AGG 0.539942 4_3:+72020829 Msa0660890:CDS
GCACCGTTGACCGCCAATCT+CGG 0.543409 4_3:+72022119 Msa0660890:CDS
ACAGTAAATGGGAGGTACCC+AGG 0.547259 4_3:+72020393 Msa0660890:CDS
CAATCCATTCTAATTGGTCC+TGG 0.548604 4_3:+72021627 Msa0660890:CDS
GGACATTGAGTAATATAAGC+TGG 0.548729 4_3:-72020646 None:intergenic
GTAGCAACAAAGTATCTGCT+TGG 0.549623 4_3:-72021687 None:intergenic
GTTGTCTAAGAGGAGCATAA+TGG 0.556107 4_3:+72019683 Msa0660890:exon
TATGCAATGGAGGTTGAGAA+AGG 0.556866 4_3:+72021483 Msa0660890:CDS
CATACAATCAATGGGAAGCC+AGG 0.558040 4_3:+72021198 Msa0660890:CDS
TAGCATAAATTGTTGGCCCT+GGG 0.558784 4_3:-72020410 None:intergenic
ATCTGTTCTACCACCACCAA+AGG 0.559203 4_3:+72022753 Msa0660890:CDS
ACATATGACTTCAATGTCAC+AGG 0.560816 4_3:+72020691 Msa0660890:CDS
TGTATGATTATATAGGTGTA+TGG 0.566550 4_3:+72022647 Msa0660890:intron
CGCCAATCTCGGTACTAAGA+CGG 0.571925 4_3:+72022130 Msa0660890:CDS
TGTCATGTTGTGACCAGCAA+GGG 0.572003 4_3:-72021562 None:intergenic
AAAGGACCTTCCAAAATGCT+AGG 0.572454 4_3:+72022771 Msa0660890:CDS
TGAAGATACATATGCAATGG+AGG 0.572819 4_3:+72021473 Msa0660890:intron
CCTATGGAGAGGAACACTGT+TGG 0.575145 4_3:+72022332 Msa0660890:CDS
AGGTTTCTATCAACTTTGAG+AGG 0.579119 4_3:-72021903 None:intergenic
CAGTAAATGGGAGGTACCCA+GGG 0.579786 4_3:+72020394 Msa0660890:CDS
CAACCAAATTATATTTAGCA+GGG 0.585738 4_3:-72022307 None:intergenic
TTCAATGTCACAGGACAAAG+AGG 0.588439 4_3:+72020700 Msa0660890:CDS
GTACCGAGATTGGCGGTCAA+CGG 0.589957 4_3:-72022122 None:intergenic
GCTCATCACTTTGATATCAA+GGG 0.590306 4_3:+72022041 Msa0660890:CDS
ATTGAAAACGGCTTTCGTTG+TGG 0.592669 4_3:+72022708 Msa0660890:CDS
CCAACAGTGTTCCTCTCCAT+AGG 0.596019 4_3:-72022332 None:intergenic
CAATGTCCAATCCAAACTGG+TGG 0.606738 4_3:+72020661 Msa0660890:CDS
TTAACAAAGATATGTGACTT+CGG 0.608585 4_3:-72019725 None:intergenic
AGAATGGTGGCACAAAGATG+TGG 0.608690 4_3:+72021119 Msa0660890:CDS
ATTGTTATCATGCCTAAACC+AGG 0.611746 4_3:+72020778 Msa0660890:CDS
CCAACAATTTATGCTACAGA+AGG 0.615473 4_3:+72020417 Msa0660890:CDS
TTCTCGGAACAAGGAAAGAG+TGG 0.616470 4_3:-72021222 None:intergenic
TTGCGGTCCAACCACCGGTA+GGG 0.617520 4_3:-72022358 None:intergenic
CGTCTTAGTACCGAGATTGG+CGG 0.619297 4_3:-72022129 None:intergenic
GGGGTTGCCACCAAATATGT+CGG 0.620199 4_3:+72021170 Msa0660890:CDS
TAAACCAAGGGAACCAAATG+GGG 0.620759 4_3:+72021151 Msa0660890:CDS
ACAGCTATATTCCAATACAA+AGG 0.622211 4_3:+72021756 Msa0660890:CDS
CTGTCATGTTGTGACCAGCA+AGG 0.622828 4_3:-72021563 None:intergenic
TATGGAGAGGAACACTGTTG+GGG 0.624128 4_3:+72022334 Msa0660890:CDS
TGGAAGAGATAGTAAACCAA+GGG 0.624447 4_3:+72021139 Msa0660890:CDS
ACTGTTGGGGTCCCTACCGG+TGG 0.626035 4_3:+72022347 Msa0660890:CDS
CGTGTTCCATTGATGCATGT+AGG 0.626273 4_3:-72021960 None:intergenic
CTTTGAGAGGAACATTAGCA+GGG 0.634352 4_3:-72021890 None:intergenic
TGTCATTTAGAGTTGCACAC+TGG 0.638629 4_3:+72022679 Msa0660890:CDS
ATTGCGGTCCAACCACCGGT+AGG 0.642154 4_3:-72022359 None:intergenic
GGACTTAAACAGTATCGCAA+TGG 0.642768 4_3:+72020610 Msa0660890:CDS
GTATAGTTAAAAGGAGCCAA+AGG 0.642857 4_3:-72022092 None:intergenic
ATTAGCCGTGAATCGAATTG+CGG 0.656457 4_3:-72022375 None:intergenic
GTGATGAGCTTGAAGAAGCG+CGG 0.657612 4_3:-72022027 None:intergenic
ATTTGGTTGATCCTATGGAG+AGG 0.658256 4_3:+72022321 Msa0660890:CDS
GTGGAAGAGATAGTAAACCA+AGG 0.661655 4_3:+72021138 Msa0660890:CDS
GAAGTGTTGTAGGAAATGAA+GGG 0.669774 4_3:-72021797 None:intergenic
ATTGTGTAACAGGAGAATGG+TGG 0.675069 4_3:+72021106 Msa0660890:intron
GACAAAGAGGAACTTTGTGG+TGG 0.676053 4_3:+72020713 Msa0660890:CDS
AGGTTGTGGAAAAGGAAAAG+GGG 0.686432 4_3:-72020803 None:intergenic
TGTGCATCCGACATATTTGG+TGG 0.699108 4_3:-72021177 None:intergenic
CAATTGTAACAGTAAATGGG+AGG 0.721812 4_3:+72020385 Msa0660890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTATAATATATATAAGTA+TGG + chr4_3:72019835-72019854 Msa0660890:intron 10.0%
!! AAATAATGATAAAAAAACAT+GGG - chr4_3:72019934-72019953 None:intergenic 10.0%
!! AAAATAATGATAAAAAAACA+TGG - chr4_3:72019935-72019954 None:intergenic 10.0%
!!! ATTATTTTTTATACGATTTT+GGG + chr4_3:72019947-72019966 Msa0660890:intron 10.0%
!!! GTTTTTGTTATATAAATTAT+AGG + chr4_3:72020584-72020603 Msa0660890:intron 10.0%
!!! TTTTTTATTTTTGTTTCTAA+AGG + chr4_3:72022551-72022570 Msa0660890:intron 10.0%
!!! CATTATTTTTTATACGATTT+TGG + chr4_3:72019946-72019965 Msa0660890:intron 15.0%
!! AAAAAATTGAATTTGTATCA+TGG - chr4_3:72020140-72020159 None:intergenic 15.0%
!!! CTTTAAAATAAATCACTTTT+AGG - chr4_3:72020277-72020296 None:intergenic 15.0%
!!! TTGATATGTTATATTTTACA+TGG - chr4_3:72020470-72020489 None:intergenic 15.0%
!!! TTTTTTACCAAACATAAATT+TGG + chr4_3:72020513-72020532 Msa0660890:intron 15.0%
!!! GTGATTTAAAACTTTTATTT+AGG + chr4_3:72021025-72021044 Msa0660890:intron 15.0%
!! TTCAAATAAACTAAATTAGA+AGG - chr4_3:72021051-72021070 None:intergenic 15.0%
!!! TTAATTAACTTAGTTTTGTT+TGG - chr4_3:72021404-72021423 None:intergenic 15.0%
!!! CAAATTACAAATTTTAATGA+GGG - chr4_3:72021449-72021468 None:intergenic 15.0%
!!! ACAAATTACAAATTTTAATG+AGG - chr4_3:72021450-72021469 None:intergenic 15.0%
!! TTAGAGATGAAAAAAAAAAT+TGG + chr4_3:72022454-72022473 Msa0660890:intron 15.0%
!! AAAATGAAAATCTTCTAAAT+TGG - chr4_3:72022483-72022502 None:intergenic 15.0%
!! ATTGTTATATTTATCTTTCT+GGG + chr4_3:72023001-72023020 Msa0660890:three_prime_UTR 15.0%
!!! TCTTTGTTAATAGTTCTTTT+TGG + chr4_3:72019738-72019757 Msa0660890:CDS 20.0%
!!! TATGTTATATTTTACATGGT+TGG - chr4_3:72020466-72020485 None:intergenic 20.0%
!! AATATAACATATCAATTCAC+TGG + chr4_3:72020475-72020494 Msa0660890:CDS 20.0%
!! TTTGGTAAAAAAGAACAAAT+TGG - chr4_3:72020505-72020524 None:intergenic 20.0%
!! TGTTATATAAATTATAGGCA+TGG + chr4_3:72020589-72020608 Msa0660890:intron 20.0%
!!! TTCAAATTTTATTGTGTAAC+AGG + chr4_3:72021096-72021115 Msa0660890:intron 20.0%
!!! AATTTTAATGAGGGAAATTT+AGG - chr4_3:72021440-72021459 None:intergenic 20.0%
!! AAATTCTTACCATATGATAT+TGG + chr4_3:72022605-72022624 Msa0660890:intron 20.0%
!!! TTTTGCATGTATGATTATAT+AGG + chr4_3:72022640-72022659 Msa0660890:intron 20.0%
!! GATTGTTATATTTATCTTTC+TGG + chr4_3:72023000-72023019 Msa0660890:three_prime_UTR 20.0%
! TTAACAAAGATATGTGACTT+CGG - chr4_3:72019728-72019747 None:intergenic 25.0%
!!! ATAGTTCTTTTTGGTTTTCT+TGG + chr4_3:72019747-72019766 Msa0660890:CDS 25.0%
!!! ATTATGTCACGTTTTAGTAA+AGG - chr4_3:72019909-72019928 None:intergenic 25.0%
! AGATGAGAATCTGAAAAAAT+AGG - chr4_3:72020221-72020240 None:intergenic 25.0%
! AAACCAATTGTAACAGTAAA+TGG + chr4_3:72020381-72020400 Msa0660890:CDS 25.0%
! AACCAATTGTAACAGTAAAT+GGG + chr4_3:72020382-72020401 Msa0660890:CDS 25.0%
! TATTTGCAACTAAAAAAGTG+CGG + chr4_3:72020880-72020899 Msa0660890:intron 25.0%
! ATAATTAGTTGAACGAGATA+AGG - chr4_3:72021378-72021397 None:intergenic 25.0%
! TTATCTCGTTCAACTAATTA+TGG + chr4_3:72021377-72021396 Msa0660890:intron 25.0%
!!! TTTTGAAGATACATATGCAA+TGG + chr4_3:72021470-72021489 Msa0660890:intron 25.0%
! TTATAACCATGAAGATGAAA+TGG - chr4_3:72022239-72022258 None:intergenic 25.0%
!!! GGTTATAACTTTTTTGTTGT+TGG + chr4_3:72022251-72022270 Msa0660890:CDS 25.0%
! CAACCAAATTATATTTAGCA+GGG - chr4_3:72022310-72022329 None:intergenic 25.0%
! TCAACCAAATTATATTTAGC+AGG - chr4_3:72022311-72022330 None:intergenic 25.0%
! ATATAATTTGGTTGATCCTA+TGG + chr4_3:72022316-72022335 Msa0660890:CDS 25.0%
!! TTTACGTCTCTAATTTTCTT+CGG + chr4_3:72022502-72022521 Msa0660890:intron 25.0%
! TGTATGATTATATAGGTGTA+TGG + chr4_3:72022647-72022666 Msa0660890:intron 25.0%
! TTAACCAAAAAGAAATTGCT+AGG + chr4_3:72022799-72022818 Msa0660890:three_prime_UTR 25.0%
CAAAACAAAAGTTGTCTAAG+AGG + chr4_3:72019673-72019692 Msa0660890:five_prime_UTR 30.0%
GCATATAACTCACATCAAAT+TGG - chr4_3:72019812-72019831 None:intergenic 30.0%
TAACATATCAATTCACTGGT+AGG + chr4_3:72020479-72020498 Msa0660890:intron 30.0%
CATGTGACCAAATTTATGTT+TGG - chr4_3:72020523-72020542 None:intergenic 30.0%
GAGATTCTAACAAGAAAACA+TGG + chr4_3:72020555-72020574 Msa0660890:intron 30.0%
ATGCTCATATTCTTTGGTTA+AGG + chr4_3:72020737-72020756 Msa0660890:CDS 30.0%
TGCTCATATTCTTTGGTTAA+GGG + chr4_3:72020738-72020757 Msa0660890:CDS 30.0%
ATACAATACAACCTCAATCA+TGG - chr4_3:72020948-72020967 None:intergenic 30.0%
TGATTGAGGTTGTATTGTAT+CGG + chr4_3:72020948-72020967 Msa0660890:intron 30.0%
TTTATTGTGTAACAGGAGAA+TGG + chr4_3:72021103-72021122 Msa0660890:intron 30.0%
GTTATTGTTCTTACGTTTCT+CGG - chr4_3:72021241-72021260 None:intergenic 30.0%
AGGTCGCTTATAAAAAATGA+CGG - chr4_3:72021281-72021300 None:intergenic 30.0%
ACAACACAATCCATTCTAAT+TGG + chr4_3:72021621-72021640 Msa0660890:CDS 30.0%
ACAGCTATATTCCAATACAA+AGG + chr4_3:72021756-72021775 Msa0660890:CDS 30.0%
! TGTTTGGAATTCCTTTGTAT+TGG - chr4_3:72021770-72021789 None:intergenic 30.0%
!! AAGTTTGTGTCATTTGCATT+AGG - chr4_3:72021822-72021841 None:intergenic 30.0%
! AGAAACCTTTTCTACACTAT+TGG + chr4_3:72021918-72021937 Msa0660890:CDS 30.0%
CTAAACCAATAGTGTAGAAA+AGG - chr4_3:72021926-72021945 None:intergenic 30.0%
!!! CTTTTCTACACTATTGGTTT+AGG + chr4_3:72021924-72021943 Msa0660890:CDS 30.0%
!!! AACTTTTTTGTTGTTGGAAC+TGG + chr4_3:72022257-72022276 Msa0660890:CDS 30.0%
AGACCCTGCTAAATATAATT+TGG + chr4_3:72022304-72022323 Msa0660890:CDS 30.0%
! TTTTAATGCAGTTGTTGACA+AGG - chr4_3:72022413-72022432 None:intergenic 30.0%
TGTCAACAACTGCATTAAAA+AGG + chr4_3:72022413-72022432 Msa0660890:intron 30.0%
TCATATGGTAAGAATTTGAC+AGG - chr4_3:72022602-72022621 None:intergenic 30.0%
! AGAATAACTGCAATTTTTGC+AGG - chr4_3:72022911-72022930 None:intergenic 30.0%
! TTCTTTATTGCTGCTTCAAC+AGG - chr4_3:72019786-72019805 None:intergenic 35.0%
!!! CTGTTACAATTGGTTTTGCA+TGG - chr4_3:72020377-72020396 None:intergenic 35.0%
CTCCCATTTACTGTTACAAT+TGG - chr4_3:72020387-72020406 None:intergenic 35.0%
CAATTGTAACAGTAAATGGG+AGG + chr4_3:72020385-72020404 Msa0660890:CDS 35.0%
CCTTCTGTAGCATAAATTGT+TGG - chr4_3:72020420-72020439 None:intergenic 35.0%
CCAACAATTTATGCTACAGA+AGG + chr4_3:72020417-72020436 Msa0660890:CDS 35.0%
TTAAACAGTATCGCAATGGT+TGG + chr4_3:72020614-72020633 Msa0660890:CDS 35.0%
TAAACAGTATCGCAATGGTT+GGG + chr4_3:72020615-72020634 Msa0660890:CDS 35.0%
GGACATTGAGTAATATAAGC+TGG - chr4_3:72020649-72020668 None:intergenic 35.0%
ACATATGACTTCAATGTCAC+AGG + chr4_3:72020691-72020710 Msa0660890:CDS 35.0%
ATTGTTATCATGCCTAAACC+AGG + chr4_3:72020778-72020797 Msa0660890:CDS 35.0%
ACAAGTTCAAATTCTCTAGC+AGG - chr4_3:72020826-72020845 None:intergenic 35.0%
AGAGAATTTGAACTTGTGCT+AGG + chr4_3:72020829-72020848 Msa0660890:CDS 35.0%
TGGAAGAGATAGTAAACCAA+GGG + chr4_3:72021139-72021158 Msa0660890:CDS 35.0%
TCGCTTATAAAAAATGACGG+AGG - chr4_3:72021278-72021297 None:intergenic 35.0%
CAAAAGAACGCTTATAACGA+GGG + chr4_3:72021311-72021330 Msa0660890:intron 35.0%
TGAAGATACATATGCAATGG+AGG + chr4_3:72021473-72021492 Msa0660890:intron 35.0%
!! AGAATGGATTGTGTTGTGAA+TGG - chr4_3:72021618-72021637 None:intergenic 35.0%
TGCTTTGACTAGAACATTTG+TGG - chr4_3:72021658-72021677 None:intergenic 35.0%
TGTTGCTACAAGAACTTTCA+TGG + chr4_3:72021701-72021720 Msa0660890:CDS 35.0%
! AATGAAGGGATGATAGTGTT+TGG - chr4_3:72021786-72021805 None:intergenic 35.0%
!! GAAGTGTTGTAGGAAATGAA+GGG - chr4_3:72021800-72021819 None:intergenic 35.0%
!! TTTGCATTAGGAAGTGTTGT+AGG - chr4_3:72021810-72021829 None:intergenic 35.0%
AGGTTTCTATCAACTTTGAG+AGG - chr4_3:72021906-72021925 None:intergenic 35.0%
AGCTCATCACTTTGATATCA+AGG + chr4_3:72022040-72022059 Msa0660890:CDS 35.0%
GCTCATCACTTTGATATCAA+GGG + chr4_3:72022041-72022060 Msa0660890:CDS 35.0%
! TGATTTTCCTGATAAGCCTT+TGG + chr4_3:72022076-72022095 Msa0660890:CDS 35.0%
GTATAGTTAAAAGGAGCCAA+AGG - chr4_3:72022095-72022114 None:intergenic 35.0%
!! TTGGCTCCTTTTAACTATAC+TGG + chr4_3:72022095-72022114 Msa0660890:CDS 35.0%
CAAGATCGCCTTTAATTCTA+CGG + chr4_3:72022166-72022185 Msa0660890:CDS 35.0%
TCACATCCATTTCATCTTCA+TGG + chr4_3:72022230-72022249 Msa0660890:CDS 35.0%
!! TTTGTTGTTGGAACTGGAAT+TGG + chr4_3:72022263-72022282 Msa0660890:CDS 35.0%
GAATTTGACAGGACAATGTA+AGG - chr4_3:72022591-72022610 None:intergenic 35.0%
ACATGCATGCCAATATCATA+TGG - chr4_3:72022617-72022636 None:intergenic 35.0%
! AGAACAGATTGATCTTTTCC+TGG - chr4_3:72022742-72022761 None:intergenic 35.0%
! GTTAAACTTGCCTAGCATTT+TGG - chr4_3:72022784-72022803 None:intergenic 35.0%
! CATGCCTAGCAATTTCTTTT+TGG - chr4_3:72022806-72022825 None:intergenic 35.0%
CAAAAAGAAATTGCTAGGCA+TGG + chr4_3:72022804-72022823 Msa0660890:three_prime_UTR 35.0%
GTCTCATAGTTCAGATTGTA+TGG + chr4_3:72022835-72022854 Msa0660890:three_prime_UTR 35.0%
! GTTGTCTAAGAGGAGCATAA+TGG + chr4_3:72019683-72019702 Msa0660890:exon 40.0%
TAGCATAAATTGTTGGCCCT+GGG - chr4_3:72020413-72020432 None:intergenic 40.0%
GGACTTAAACAGTATCGCAA+TGG + chr4_3:72020610-72020629 Msa0660890:CDS 40.0%
ACTCAATGTCCAATCCAAAC+TGG + chr4_3:72020658-72020677 Msa0660890:CDS 40.0%
ATGTATAGCTTCCACCAGTT+TGG - chr4_3:72020675-72020694 None:intergenic 40.0%
TTCAATGTCACAGGACAAAG+AGG + chr4_3:72020700-72020719 Msa0660890:CDS 40.0%
TGGTTAAGGGCTACTGTTTA+TGG + chr4_3:72020751-72020770 Msa0660890:CDS 40.0%
AGGTTGTGGAAAAGGAAAAG+GGG - chr4_3:72020806-72020825 None:intergenic 40.0%
CAGGTTGTGGAAAAGGAAAA+GGG - chr4_3:72020807-72020826 None:intergenic 40.0%
TCAAATTCTCTAGCAGGTTG+TGG - chr4_3:72020820-72020839 None:intergenic 40.0%
TACAACCTCAATCATGGTCA+TGG - chr4_3:72020942-72020961 None:intergenic 40.0%
ATTGTGTAACAGGAGAATGG+TGG + chr4_3:72021106-72021125 Msa0660890:intron 40.0%
GTGGAAGAGATAGTAAACCA+AGG + chr4_3:72021138-72021157 Msa0660890:CDS 40.0%
AGTAAACCAAGGGAACCAAA+TGG + chr4_3:72021149-72021168 Msa0660890:CDS 40.0%
GTAAACCAAGGGAACCAAAT+GGG + chr4_3:72021150-72021169 Msa0660890:CDS 40.0%
TAAACCAAGGGAACCAAATG+GGG + chr4_3:72021151-72021170 Msa0660890:CDS 40.0%
GTATGTGCATCCGACATATT+TGG - chr4_3:72021183-72021202 None:intergenic 40.0%
TCGGATGCACATACAATCAA+TGG + chr4_3:72021189-72021208 Msa0660890:CDS 40.0%
CGGATGCACATACAATCAAT+GGG + chr4_3:72021190-72021209 Msa0660890:CDS 40.0%
TTCTTACGTTTCTCGGAACA+AGG - chr4_3:72021234-72021253 None:intergenic 40.0%
! CGTTCTTTTGCACTCACAAA+AGG - chr4_3:72021301-72021320 None:intergenic 40.0%
GCAAAAGAACGCTTATAACG+AGG + chr4_3:72021310-72021329 Msa0660890:intron 40.0%
TATGCAATGGAGGTTGAGAA+AGG + chr4_3:72021483-72021502 Msa0660890:CDS 40.0%
! GAACTTTTCTTTTCCCTTGC+TGG + chr4_3:72021549-72021568 Msa0660890:CDS 40.0%
CAATCCATTCTAATTGGTCC+TGG + chr4_3:72021627-72021646 Msa0660890:CDS 40.0%
TTGACCAGGACCAATTAGAA+TGG - chr4_3:72021634-72021653 None:intergenic 40.0%
AGAACATTTGTGGTTTGACC+AGG - chr4_3:72021648-72021667 None:intergenic 40.0%
! GTAGCAACAAAGTATCTGCT+TGG - chr4_3:72021690-72021709 None:intergenic 40.0%
!! GTTTTGTTATCAACCGAGAG+TGG - chr4_3:72021732-72021751 None:intergenic 40.0%
!! GGAAGTGTTGTAGGAAATGA+AGG - chr4_3:72021801-72021820 None:intergenic 40.0%
! CTTTGAGAGGAACATTAGCA+GGG - chr4_3:72021893-72021912 None:intergenic 40.0%
! ACTTTGAGAGGAACATTAGC+AGG - chr4_3:72021894-72021913 None:intergenic 40.0%
TCTTGTCCTACATGCATCAA+TGG + chr4_3:72021954-72021973 Msa0660890:CDS 40.0%
CAGCTCAACCGTAGAATTAA+AGG - chr4_3:72022177-72022196 None:intergenic 40.0%
CTTTAATTCTACGGTTGAGC+TGG + chr4_3:72022175-72022194 Msa0660890:CDS 40.0%
TATTTAGCAGGGTCTTTAGC+AGG - chr4_3:72022299-72022318 None:intergenic 40.0%
ATTTGGTTGATCCTATGGAG+AGG + chr4_3:72022321-72022340 Msa0660890:CDS 40.0%
ATTAGCCGTGAATCGAATTG+CGG - chr4_3:72022378-72022397 None:intergenic 40.0%
AGTTGTTGACAAGGTATACC+TGG - chr4_3:72022404-72022423 None:intergenic 40.0%
! TGTCATTTAGAGTTGCACAC+TGG + chr4_3:72022679-72022698 Msa0660890:CDS 40.0%
! ATTTAGAGTTGCACACTGGT+TGG + chr4_3:72022683-72022702 Msa0660890:CDS 40.0%
! TTTAGAGTTGCACACTGGTT+GGG + chr4_3:72022684-72022703 Msa0660890:CDS 40.0%
ATTGAAAACGGCTTTCGTTG+TGG + chr4_3:72022708-72022727 Msa0660890:CDS 40.0%
!! TAGCATTTTGGAAGGTCCTT+TGG - chr4_3:72022772-72022791 None:intergenic 40.0%
AAAGGACCTTCCAAAATGCT+AGG + chr4_3:72022771-72022790 Msa0660890:CDS 40.0%
! AACTTGCCTAGCATTTTGGA+AGG - chr4_3:72022780-72022799 None:intergenic 40.0%
GTAGCATAAATTGTTGGCCC+TGG - chr4_3:72020414-72020433 None:intergenic 45.0%
CAATGTCCAATCCAAACTGG+TGG + chr4_3:72020661-72020680 Msa0660890:CDS 45.0%
TAGCTTCCACCAGTTTGGAT+TGG - chr4_3:72020670-72020689 None:intergenic 45.0%
CAGGACAAAGAGGAACTTTG+TGG + chr4_3:72020710-72020729 Msa0660890:CDS 45.0%
GACAAAGAGGAACTTTGTGG+TGG + chr4_3:72020713-72020732 Msa0660890:CDS 45.0%
GGTGGCATGCTCATATTCTT+TGG + chr4_3:72020731-72020750 Msa0660890:CDS 45.0%
GCAGGTTGTGGAAAAGGAAA+AGG - chr4_3:72020808-72020827 None:intergenic 45.0%
TCTCTAGCAGGTTGTGGAAA+AGG - chr4_3:72020814-72020833 None:intergenic 45.0%
GATCTCCATGACCATGATTG+AGG + chr4_3:72020934-72020953 Msa0660890:intron 45.0%
AGAATGGTGGCACAAAGATG+TGG + chr4_3:72021119-72021138 Msa0660890:CDS 45.0%
! TATTTGGTGGCAACCCCATT+TGG - chr4_3:72021167-72021186 None:intergenic 45.0%
TGTGCATCCGACATATTTGG+TGG - chr4_3:72021180-72021199 None:intergenic 45.0%
CATACAATCAATGGGAAGCC+AGG + chr4_3:72021198-72021217 Msa0660890:CDS 45.0%
TTCTCGGAACAAGGAAAGAG+TGG - chr4_3:72021225-72021244 None:intergenic 45.0%
GACGGAGGTAGTACAAACTT+GGG - chr4_3:72021263-72021282 None:intergenic 45.0%
TGACGGAGGTAGTACAAACT+TGG - chr4_3:72021264-72021283 None:intergenic 45.0%
TGTCATGTTGTGACCAGCAA+GGG - chr4_3:72021565-72021584 None:intergenic 45.0%
TTTCATGGATGCACCACTCT+CGG + chr4_3:72021716-72021735 Msa0660890:CDS 45.0%
CGTGTTCCATTGATGCATGT+AGG - chr4_3:72021963-72021982 None:intergenic 45.0%
AAAGGAGCCAAAGGCTTATC+AGG - chr4_3:72022086-72022105 None:intergenic 45.0%
GGTGCGCCAGTATAGTTAAA+AGG - chr4_3:72022104-72022123 None:intergenic 45.0%
CTATGGAGAGGAACACTGTT+GGG + chr4_3:72022333-72022352 Msa0660890:CDS 45.0%
TATGGAGAGGAACACTGTTG+GGG + chr4_3:72022334-72022353 Msa0660890:CDS 45.0%
TTGGACCGCAATTCGATTCA+CGG + chr4_3:72022370-72022389 Msa0660890:CDS 45.0%
CGATTCACGGCTAATAATCC+AGG + chr4_3:72022383-72022402 Msa0660890:CDS 45.0%
! TTAGAGTTGCACACTGGTTG+GGG + chr4_3:72022685-72022704 Msa0660890:CDS 45.0%
! CACTGGTTGGGGATTGAAAA+CGG + chr4_3:72022696-72022715 Msa0660890:CDS 45.0%
ATCTGTTCTACCACCACCAA+AGG + chr4_3:72022753-72022772 Msa0660890:CDS 45.0%
!! CATTTTGGAAGGTCCTTTGG+TGG - chr4_3:72022769-72022788 None:intergenic 45.0%
!! ACAGTAAATGGGAGGTACCC+AGG + chr4_3:72020393-72020412 Msa0660890:CDS 50.0%
!! CAGTAAATGGGAGGTACCCA+GGG + chr4_3:72020394-72020413 Msa0660890:CDS 50.0%
TATCGCAATGGTTGGGCTGA+TGG + chr4_3:72020622-72020641 Msa0660890:CDS 50.0%
GGAAAAGGGGTTCCTGGTTT+AGG - chr4_3:72020793-72020812 None:intergenic 50.0%
GGAAAAGGAAAAGGGGTTCC+TGG - chr4_3:72020799-72020818 None:intergenic 50.0%
GGGGTTGCCACCAAATATGT+CGG + chr4_3:72021170-72021189 Msa0660890:CDS 50.0%
GAACAAGGAAAGAGTGGTCC+TGG - chr4_3:72021219-72021238 None:intergenic 50.0%
CTGTCATGTTGTGACCAGCA+AGG - chr4_3:72021566-72021585 None:intergenic 50.0%
!! GCTTGAAGAAGCGCGGTTTT+CGG - chr4_3:72022023-72022042 None:intergenic 50.0%
GTGATGAGCTTGAAGAAGCG+CGG - chr4_3:72022030-72022049 None:intergenic 50.0%
! CGTCTTAGTACCGAGATTGG+CGG - chr4_3:72022132-72022151 None:intergenic 50.0%
CGCCAATCTCGGTACTAAGA+CGG + chr4_3:72022130-72022149 Msa0660890:CDS 50.0%
GCCAATCTCGGTACTAAGAC+GGG + chr4_3:72022131-72022150 Msa0660890:CDS 50.0%
CCAACAGTGTTCCTCTCCAT+AGG - chr4_3:72022335-72022354 None:intergenic 50.0%
CCTATGGAGAGGAACACTGT+TGG + chr4_3:72022332-72022351 Msa0660890:CDS 50.0%
ACGGCTTTCGTTGTGGAAGA+TGG + chr4_3:72022715-72022734 Msa0660890:CDS 50.0%
CGGCTTTCGTTGTGGAAGAT+GGG + chr4_3:72022716-72022735 Msa0660890:CDS 50.0%
TTTGGAAGGTCCTTTGGTGG+TGG - chr4_3:72022766-72022785 None:intergenic 50.0%
! GCAACCCCATTTGGTTCCCT+TGG - chr4_3:72021158-72021177 None:intergenic 55.0%
GTACCGAGATTGGCGGTCAA+CGG - chr4_3:72022125-72022144 None:intergenic 55.0%
! GCCCGTCTTAGTACCGAGAT+TGG - chr4_3:72022135-72022154 None:intergenic 55.0%
AACACTGTTGGGGTCCCTAC+CGG + chr4_3:72022344-72022363 Msa0660890:CDS 55.0%
TCGAATTGCGGTCCAACCAC+CGG - chr4_3:72022366-72022385 None:intergenic 55.0%
TTCGTTGTGGAAGATGGGCC+AGG + chr4_3:72022721-72022740 Msa0660890:CDS 55.0%
GCACCGTTGACCGCCAATCT+CGG + chr4_3:72022119-72022138 Msa0660890:CDS 60.0%
TTGCGGTCCAACCACCGGTA+GGG - chr4_3:72022361-72022380 None:intergenic 60.0%
ATTGCGGTCCAACCACCGGT+AGG - chr4_3:72022362-72022381 None:intergenic 60.0%
ACTGTTGGGGTCCCTACCGG+TGG + chr4_3:72022347-72022366 Msa0660890:CDS 65.0%
TTGGGGTCCCTACCGGTGGT+TGG + chr4_3:72022351-72022370 Msa0660890:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 72019641 72023047 72019641 ID=Msa0660890;Name=Msa0660890
chr4_3 mRNA 72019641 72023047 72019641 ID=Msa0660890-mRNA-1;Parent=Msa0660890;Name=Msa0660890-mRNA-1;_AED=0.06;_eAED=0.06;_QI=61|0.8|0.83|1|1|1|6|255|572
chr4_3 exon 72019641 72019818 72019641 ID=Msa0660890-mRNA-1:exon:21568;Parent=Msa0660890-mRNA-1
chr4_3 exon 72020345 72020496 72020345 ID=Msa0660890-mRNA-1:exon:21569;Parent=Msa0660890-mRNA-1
chr4_3 exon 72020606 72020850 72020606 ID=Msa0660890-mRNA-1:exon:21570;Parent=Msa0660890-mRNA-1
chr4_3 exon 72021118 72021264 72021118 ID=Msa0660890-mRNA-1:exon:21571;Parent=Msa0660890-mRNA-1
chr4_3 exon 72021478 72022404 72021478 ID=Msa0660890-mRNA-1:exon:21572;Parent=Msa0660890-mRNA-1
chr4_3 exon 72022662 72023047 72022662 ID=Msa0660890-mRNA-1:exon:21573;Parent=Msa0660890-mRNA-1
chr4_3 five_prime_UTR 72019641 72019701 72019641 ID=Msa0660890-mRNA-1:five_prime_utr;Parent=Msa0660890-mRNA-1
chr4_3 CDS 72019702 72019818 72019702 ID=Msa0660890-mRNA-1:cds;Parent=Msa0660890-mRNA-1
chr4_3 CDS 72020345 72020496 72020345 ID=Msa0660890-mRNA-1:cds;Parent=Msa0660890-mRNA-1
chr4_3 CDS 72020606 72020850 72020606 ID=Msa0660890-mRNA-1:cds;Parent=Msa0660890-mRNA-1
chr4_3 CDS 72021118 72021264 72021118 ID=Msa0660890-mRNA-1:cds;Parent=Msa0660890-mRNA-1
chr4_3 CDS 72021478 72022404 72021478 ID=Msa0660890-mRNA-1:cds;Parent=Msa0660890-mRNA-1
chr4_3 CDS 72022662 72022792 72022662 ID=Msa0660890-mRNA-1:cds;Parent=Msa0660890-mRNA-1
chr4_3 three_prime_UTR 72022793 72023047 72022793 ID=Msa0660890-mRNA-1:three_prime_utr;Parent=Msa0660890-mRNA-1
Gene Sequence

>Msa0660890

ATGGCTTATCATATGATGAGCTTCCGAAGTCACATATCTTTGTTAATAGTTCTTTTTGGTTTTCTTGGCTTAATCTCTTTTCCTGTTGAAGCAGCAATAAAGAAATACCAATTTGATATTCAAATGAAGAATGTGAGTAGACTATGCCATGCAAAACCAATTGTAACAGTAAATGGGAGGTACCCAGGGCCAACAATTTATGCTACAGAAGGAGATAGAGTACAAATCAATGTCACCAACCATGTAAAATATAACATATCAATTCACTGGCATGGACTTAAACAGTATCGCAATGGTTGGGCTGATGGACCAGCTTATATTACTCAATGTCCAATCCAAACTGGTGGAAGCTATACATATGACTTCAATGTCACAGGACAAAGAGGAACTTTGTGGTGGCATGCTCATATTCTTTGGTTAAGGGCTACTGTTTATGGTGCAATTGTTATCATGCCTAAACCAGGAACCCCTTTTCCTTTTCCACAACCTGCTAGAGAATTTGAACTTGTGCTAGGAGAATGGTGGCACAAAGATGTGGAAGAGATAGTAAACCAAGGGAACCAAATGGGGTTGCCACCAAATATGTCGGATGCACATACAATCAATGGGAAGCCAGGACCACTCTTTCCTTGTTCCGAGAAACGTAAGAACAATAACCCAAATACATATGCAATGGAGGTTGAGAAAGGCAAGACATATCTGCTAAGAATCATCAATGCTGCTCTTAATGATGAACTTTTCTTTTCCCTTGCTGGTCACAACATGACAGTAGTTGAAGTTGATGCAGTTTACACAAAACCATTCACAACACAATCCATTCTAATTGGTCCTGGTCAAACCACAAATGTTCTAGTCAAAGCAAACAAAATTCCAAGCAGATACTTTGTTGCTACAAGAACTTTCATGGATGCACCACTCTCGGTTGATAACAAAACTGCTACAGCTATATTCCAATACAAAGGAATTCCAAACACTATCATCCCTTCATTTCCTACAACACTTCCTAATGCAAATGACACAAACTTTGCTTTGAATTATAGCAAAAAAATAAGAAGCTTAAACTCTGCTAAATACCCTGCTAATGTTCCTCTCAAAGTTGATAGAAACCTTTTCTACACTATTGGTTTAGGCATGAATTCTTGTCCTACATGCATCAATGGAACACGTTTAGTTGCATCACTTAACAATGTAACTTTTGTGATGCCGAAAACCGCGCTTCTTCAAGCTCATCACTTTGATATCAAGGGTGTTTTTAGAACTGATTTTCCTGATAAGCCTTTGGCTCCTTTTAACTATACTGGCGCACCGTTGACCGCCAATCTCGGTACTAAGACGGGCACAAGAGTTAGCAAGATCGCCTTTAATTCTACGGTTGAGCTGGTTTTGCAAGATACTAATCTTCTCACAGTTGAATCACATCCATTTCATCTTCATGGTTATAACTTTTTTGTTGTTGGAACTGGAATTGGCAATTTTGATCCTGCTAAAGACCCTGCTAAATATAATTTGGTTGATCCTATGGAGAGGAACACTGTTGGGGTCCCTACCGGTGGTTGGACCGCAATTCGATTCACGGCTAATAATCCAGGTGTATGGTTCATGCATTGTCATTTAGAGTTGCACACTGGTTGGGGATTGAAAACGGCTTTCGTTGTGGAAGATGGGCCAGGAAAAGATCAATCTGTTCTACCACCACCAAAGGACCTTCCAAAATGCTAG
Protein sequence

>Msa0660890

MAYHMMSFRSHISLLIVLFGFLGLISFPVEAAIKKYQFDIQMKNVSRLCHAKPIVTVNGRYPGPTIYATEGDRVQINVTNHVKYNISIHWHGLKQYRNGWADGPAYITQCPIQTGGSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKPGTPFPFPQPAREFELVLGEWWHKDVEEIVNQGNQMGLPPNMSDAHTINGKPGPLFPCSEKRKNNNPNTYAMEVEKGKTYLLRIINAALNDELFFSLAGHNMTVVEVDAVYTKPFTTQSILIGPGQTTNVLVKANKIPSRYFVATRTFMDAPLSVDNKTATAIFQYKGIPNTIIPSFPTTLPNANDTNFALNYSKKIRSLNSAKYPANVPLKVDRNLFYTIGLGMNSCPTCINGTRLVASLNNVTFVMPKTALLQAHHFDIKGVFRTDFPDKPLAPFNYTGAPLTANLGTKTGTRVSKIAFNSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFDPAKDPAKYNLVDPMERNTVGVPTGGWTAIRFTANNPGVWFMHCHLELHTGWGLKTAFVVEDGPGKDQSVLPPPKDLPKC*