AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0716710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0716710 MtrunA17_Chr5g0404341 98.077 572 5 1 1 572 1 566 0.0 1160
Msa0716710 MtrunA17_Chr5g0404361 82.250 569 92 4 5 572 51 611 0.0 981
Msa0716710 MtrunA17_Chr5g0429511 60.215 558 215 2 15 572 8 558 0.0 723
Msa0716710 MtrunA17_Chr3g0104061 62.500 544 196 3 29 572 21 556 0.0 722
Msa0716710 MtrunA17_Chr4g0005921 60.213 563 215 3 12 572 2 557 0.0 696
Msa0716710 MtrunA17_Chr5g0436461 56.014 557 229 4 21 572 9 554 0.0 673
Msa0716710 MtrunA17_Chr7g0238981 57.989 557 212 8 31 572 44 593 0.0 663
Msa0716710 MtrunA17_Chr7g0237491 54.188 585 243 8 6 572 3 580 0.0 649
Msa0716710 MtrunA17_Chr7g0237441 54.592 588 243 9 1 572 1 580 0.0 648
Msa0716710 MtrunA17_Chr7g0238351 55.102 588 236 9 6 572 1 581 0.0 648
Msa0716710 MtrunA17_Chr7g0238941 53.082 584 252 9 4 572 2 578 0.0 637
Msa0716710 MtrunA17_Chr4g0031901 53.235 541 234 6 31 560 41 573 0.0 607
Msa0716710 MtrunA17_Chr5g0437681 48.973 584 265 8 5 572 1 567 0.0 587
Msa0716710 MtrunA17_Chr3g0114211 49.486 584 265 9 5 572 1 570 0.0 580
Msa0716710 MtrunA17_Chr5g0431581 51.399 572 252 9 13 572 3 560 0.0 577
Msa0716710 MtrunA17_Chr2g0278811 44.658 571 285 6 33 572 37 607 6.24e-177 513
Msa0716710 MtrunA17_Chr4g0008521 44.784 556 283 6 33 572 25 572 5.25e-174 504
Msa0716710 MtrunA17_Chr8g0346581 42.295 584 312 8 1 572 1 571 2.28e-170 495
Msa0716710 MtrunA17_Chr7g0240991 44.014 568 292 11 22 572 13 571 1.09e-163 478
Msa0716710 MtrunA17_Chr7g0240971 44.077 574 296 8 14 572 7 570 2.46e-156 459
Msa0716710 MtrunA17_Chr3g0133861 46.769 325 160 9 242 555 1 323 6.89e-86 270
Msa0716710 MtrunA17_Chr8g0382691 33.942 548 318 16 29 555 29 553 9.62e-83 269
Msa0716710 MtrunA17_Chr5g0436431 52.155 232 104 2 302 530 1 228 8.22e-80 250
Msa0716710 MtrunA17_Chr3g0118321 31.818 594 340 22 12 572 1 562 6.23e-70 235
Msa0716710 MtrunA17_Chr3g0118331 30.580 569 340 19 12 550 1 544 5.49e-65 222
Msa0716710 MtrunA17_Chr3g0133871 47.642 212 110 1 6 216 3 214 5.82e-63 206
Msa0716710 MtrunA17_Chr4g0038671 29.268 574 339 18 15 572 10 532 1.59e-58 204
Msa0716710 MtrunA17_Chr4g0056511 28.920 574 369 17 10 572 4 549 4.01e-58 204
Msa0716710 MtrunA17_Chr3g0143671 29.234 561 335 18 27 572 20 533 1.42e-57 201
Msa0716710 MtrunA17_Chr1g0157341 28.147 572 353 16 14 572 6 532 1.46e-54 193
Msa0716710 MtrunA17_Chr3g0079321 27.624 543 356 16 5 537 1 516 6.21e-54 192
Msa0716710 MtrunA17_Chr4g0024861 29.903 515 302 18 30 532 23 490 2.64e-53 190
Msa0716710 MtrunA17_Chr2g0322441 31.055 512 300 17 30 532 26 493 1.72e-49 179
Msa0716710 MtrunA17_Chr7g0234061 28.180 511 331 16 36 537 31 514 5.20e-49 179
Msa0716710 MtrunA17_Chr8g0374271 28.983 521 300 19 29 532 26 493 1.18e-48 177
Msa0716710 MtrunA17_Chr8g0374261 27.869 549 309 21 17 538 9 497 1.34e-47 174
Msa0716710 MtrunA17_Chr5g0402831 28.107 523 311 18 29 538 26 496 5.93e-46 169
Msa0716710 MtrunA17_Chr4g0043261 27.556 577 355 20 15 571 6 539 1.14e-44 166
Msa0716710 MtrunA17_Chr4g0075071 30.427 539 302 20 13 532 10 494 1.27e-43 163
Msa0716710 MtrunA17_Chr3g0121161 27.163 578 363 22 13 571 46 584 2.28e-43 163
Msa0716710 MtrunA17_Chr3g0121151 27.163 578 363 22 13 571 46 584 5.18e-43 162
Msa0716710 MtrunA17_Chr3g0121171 27.194 581 359 23 13 571 1156 1694 2.62e-42 163
Msa0716710 MtrunA17_Chr3g0121171 27.163 578 363 22 13 571 581 1119 5.13e-42 162
Msa0716710 MtrunA17_Chr3g0121171 27.568 555 345 21 36 571 28 544 9.76e-42 162
Msa0716710 MtrunA17_Chr3g0121171 27.652 528 325 21 63 571 1771 2260 2.64e-36 145
Msa0716710 MtrunA17_Chr4g0024641 29.630 459 261 20 88 532 3 413 1.29e-37 149
Msa0716710 MtrunA17_Chr4g0043251 27.106 546 327 23 52 571 45 545 3.04e-37 145
Msa0716710 MtrunA17_Chr3g0118351 32.416 327 178 11 253 551 6 317 4.36e-31 124
Msa0716710 MtrunA17_Chr1g0197301 27.978 361 213 14 227 572 1 329 3.47e-27 112
Msa0716710 MtrunA17_Chr3g0118341 34.483 174 106 6 12 181 4 173 3.40e-24 100
Msa0716710 MtrunA17_Chr5g0436411 46.479 71 36 1 21 89 9 79 1.33e-16 74.7
Msa0716710 MtrunA17_Chr1g0197311 28.251 223 112 8 44 231 34 243 2.21e-16 79.0
Msa0716710 MtrunA17_Chr4g0075781 29.518 166 94 5 142 302 5 152 1.14e-13 69.3
Msa0716710 MtrunA17_Chr3g0118931 38.462 91 51 2 482 572 10 95 3.50e-11 60.5
Msa0716710 MtrunA17_Chr8g0370581 82.143 28 5 0 189 216 1 28 3.95e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0716710 AT5G03260.1 75.313 559 129 3 14 572 8 557 0.0 889
Msa0716710 AT2G38080.1 60.708 565 211 4 10 572 3 558 0.0 729
Msa0716710 AT5G58910.2 58.699 569 224 4 9 572 5 567 0.0 699
Msa0716710 AT5G58910.3 58.699 569 223 5 9 572 5 566 0.0 695
Msa0716710 AT5G58910.1 60.075 536 196 5 42 572 1 523 0.0 667
Msa0716710 AT5G01190.1 57.904 563 221 7 15 572 7 558 0.0 655
Msa0716710 AT5G60020.1 54.124 582 239 8 12 572 3 577 0.0 646
Msa0716710 AT2G29130.1 54.007 574 237 10 17 572 9 573 0.0 635
Msa0716710 AT5G60020.3 55.797 552 216 8 42 572 1 545 0.0 633
Msa0716710 AT5G01190.2 57.016 563 212 8 15 572 7 544 0.0 632
Msa0716710 AT5G05390.1 50.704 568 257 9 17 572 9 565 0.0 588
Msa0716710 AT5G60020.2 52.963 540 226 8 12 530 3 535 0.0 578
Msa0716710 AT1G18140.1 49.644 562 257 8 31 572 26 581 0.0 578
Msa0716710 AT2G30210.1 49.228 583 268 8 5 572 1 570 0.0 575
Msa0716710 AT2G40370.1 47.692 585 268 9 15 572 7 580 0.0 556
Msa0716710 AT5G07130.1 48.205 585 270 10 5 572 1 569 0.0 548
Msa0716710 AT5G09360.1 47.100 569 279 10 13 572 14 569 0.0 543
Msa0716710 AT3G09220.1 45.714 560 285 7 5 555 1 550 3.85e-172 500
Msa0716710 AT2G46570.1 44.704 557 281 10 29 572 27 569 6.78e-172 499
Msa0716710 AT5G07130.2 50.101 493 219 9 97 572 2 484 5.93e-166 481
Msa0716710 AT5G07130.3 50.101 493 219 9 97 572 2 484 5.93e-166 481
Msa0716710 AT5G01040.1 43.772 578 297 9 9 572 3 566 4.42e-164 480
Msa0716710 AT5G01050.1 43.523 579 299 9 9 572 3 568 5.98e-164 479
Msa0716710 AT5G48100.1 41.993 562 296 12 14 562 9 553 1.72e-152 449
Msa0716710 AT2G46570.2 44.242 495 249 10 91 572 4 484 6.46e-148 435
Msa0716710 AT3G09220.2 46.507 458 230 6 107 555 2 453 1.11e-142 421
Msa0716710 AT4G39830.1 32.215 596 337 20 12 572 14 577 1.18e-83 272
Msa0716710 AT5G21105.1 32.874 581 338 19 1 551 1 559 1.83e-79 261
Msa0716710 AT5G21100.1 31.743 545 325 14 31 551 21 542 5.82e-76 252
Msa0716710 AT5G21105.3 32.321 560 327 19 1 530 1 538 1.35e-70 237
Msa0716710 AT4G39830.2 32.742 507 275 19 100 572 8 482 7.64e-63 214
Msa0716710 AT4G39830.3 32.742 507 275 19 100 572 6 480 7.69e-63 214
Msa0716710 AT4G39830.4 32.283 508 263 18 100 572 8 469 2.30e-59 205
Msa0716710 AT1G41830.1 28.055 581 354 18 5 572 2 531 5.31e-57 200
Msa0716710 AT1G76160.1 28.219 567 349 15 17 572 11 530 1.11e-56 199
Msa0716710 AT4G12420.1 28.008 532 348 15 16 537 6 512 1.87e-56 200
Msa0716710 AT4G12420.2 28.008 532 348 15 16 537 6 512 1.87e-56 200
Msa0716710 AT4G25240.2 28.328 586 366 20 5 572 1 550 6.03e-56 198
Msa0716710 AT4G25240.1 28.328 586 366 20 5 572 1 550 6.03e-56 198
Msa0716710 AT1G21850.1 28.571 539 315 17 17 538 11 496 2.18e-55 196
Msa0716710 AT5G51480.1 27.336 578 355 20 17 572 16 550 1.41e-54 195
Msa0716710 AT1G75790.1 29.508 549 327 16 31 564 24 527 2.11e-54 193
Msa0716710 AT5G66920.1 28.699 561 327 19 30 572 33 538 3.73e-54 192
Msa0716710 AT1G21860.1 28.364 550 328 17 1 538 1 496 6.10e-54 192
Msa0716710 AT4G28090.1 27.820 532 324 15 55 572 49 534 1.31e-53 191
Msa0716710 AT4G22010.1 29.361 579 337 20 10 572 8 530 2.93e-52 187
Msa0716710 AT4G38420.1 28.327 526 319 16 22 532 17 499 2.26e-51 185
Msa0716710 AT5G48450.2 28.684 509 313 17 36 532 31 501 5.79e-50 182
Msa0716710 AT5G48450.1 28.684 509 313 17 36 532 31 501 1.79e-49 181
Msa0716710 AT4G38420.3 28.150 508 307 16 22 514 17 481 4.31e-47 172
Msa0716710 AT5G21105.2 34.234 333 198 9 225 551 51 368 1.24e-44 163
Msa0716710 AT4G38420.2 27.708 480 290 15 22 486 17 454 2.89e-43 160
Msa0716710 AT2G23630.1 29.341 501 290 19 52 538 48 498 1.10e-42 161
Msa0716710 AT2G23630.2 29.341 501 290 19 52 538 53 503 1.29e-42 160
Msa0716710 AT4G37160.1 26.617 541 330 19 14 538 11 500 1.88e-41 157
Msa0716710 AT1G55560.1 28.283 495 299 21 52 532 44 496 5.92e-38 147
Msa0716710 AT1G55570.1 26.829 492 311 16 52 532 47 500 1.77e-36 143
Msa0716710 AT3G13400.2 27.202 511 316 20 36 532 29 497 1.35e-35 140
Msa0716710 AT3G13400.1 27.202 511 316 20 36 532 29 497 1.35e-35 140
Msa0716710 AT3G13390.1 26.190 504 327 16 38 532 32 499 3.41e-34 137
Msa0716710 AT4G37160.2 26.176 489 295 18 14 486 11 449 2.77e-33 132

Find 143 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTGTTATATTTATCTTTC+TGG 0.115926 5_1:-10182368 Msa0716710:three_prime_UTR
AAACCAATTGTAACAGTAAA+TGG 0.140664 5_1:-10184987 Msa0716710:CDS
TGGTTAAGGGCTACTGTTTA+TGG 0.146954 5_1:-10184617 Msa0716710:CDS
AGACCCTGCTAAATATAATT+TGG 0.195177 5_1:-10183064 Msa0716710:CDS
GTTAAACTTGCCTAGCATTT+TGG 0.196468 5_1:+10182587 None:intergenic
CAGCTCAACCGTAGAATTAA+AGG 0.252917 5_1:+10183194 None:intergenic
TCACATCCATTTCATCTTCA+TGG 0.290780 5_1:-10183138 Msa0716710:CDS
ACAACACAATCCATTCTAAT+TGG 0.290849 5_1:-10183747 Msa0716710:CDS
ATGCTCATATTCTTTGGTTA+AGG 0.305616 5_1:-10184631 Msa0716710:CDS
GCATATAACTCACATCAAAT+TGG 0.309196 5_1:+10185559 None:intergenic
TGTTGCTACAAGAACTTTCA+TGG 0.327046 5_1:-10183667 Msa0716710:CDS
TTCTTTATTGCTGCTTCAAC+AGG 0.329252 5_1:+10185585 None:intergenic
GGAAAAGGGGTTCCTGGTTT+AGG 0.331002 5_1:+10184578 None:intergenic
GTATGTGCATCCGACATATT+TGG 0.355480 5_1:+10184188 None:intergenic
TGTTTGGAATTCCTTTGTAT+TGG 0.355508 5_1:+10183601 None:intergenic
GGTGGCATGCTCATATTCTT+TGG 0.356615 5_1:-10184637 Msa0716710:CDS
AAAGGAGCCAAAGGCTTATC+AGG 0.358204 5_1:+10183285 None:intergenic
ATTGTTATATTTATCTTTCT+GGG 0.364752 5_1:-10182367 Msa0716710:three_prime_UTR
CTCCCATTTACTGTTACAAT+TGG 0.366040 5_1:+10184984 None:intergenic
TGCTCATATTCTTTGGTTAA+GGG 0.367150 5_1:-10184630 Msa0716710:CDS
TTTGTTGTTGGAACTGGAAT+TGG 0.368677 5_1:-10183105 Msa0716710:CDS
TTTAGAGTTGCACACTGGTT+GGG 0.368974 5_1:-10182684 Msa0716710:CDS
GTCTCATAGTTCAGATTGTA+TGG 0.369346 5_1:-10182533 Msa0716710:three_prime_UTR
GTTATTGTTCTTACGTTTCT+CGG 0.370163 5_1:+10184130 None:intergenic
ATGTATAGCTTCCACCAGTT+TGG 0.371631 5_1:+10184696 None:intergenic
GGTGCGCCAGTATAGTTAAA+AGG 0.375538 5_1:+10183267 None:intergenic
GCAACCCCATTTGGTTCCCT+TGG 0.381589 5_1:+10184213 None:intergenic
AAGTTTGTGTCATTTGCATT+AGG 0.383453 5_1:+10183549 None:intergenic
ATATAATTTGGTTGATCCTA+TGG 0.386113 5_1:-10183052 Msa0716710:CDS
AGCTCATCACTTTGATATCA+AGG 0.386540 5_1:-10183328 Msa0716710:CDS
GGAAAAGGAAAAGGGGTTCC+TGG 0.388932 5_1:+10184572 None:intergenic
CTATGGAGAGGAACACTGTT+GGG 0.398198 5_1:-10183035 Msa0716710:CDS
ACGGCTTTCGTTGTGGAAGA+TGG 0.405749 5_1:-10182653 Msa0716710:CDS
TGCTTTGACTAGAACATTTG+TGG 0.409362 5_1:+10183713 None:intergenic
TCAACCAAATTATATTTAGC+AGG 0.412948 5_1:+10183060 None:intergenic
TCTCTAGCAGGTTGTGGAAA+AGG 0.413807 5_1:+10184557 None:intergenic
TTATAACCATGAAGATGAAA+TGG 0.419238 5_1:+10183132 None:intergenic
AATGAAGGGATGATAGTGTT+TGG 0.419479 5_1:+10183585 None:intergenic
CACTGGTTGGGGATTGAAAA+CGG 0.424322 5_1:-10182672 Msa0716710:CDS
CTTTAATTCTACGGTTGAGC+TGG 0.424324 5_1:-10183193 Msa0716710:CDS
TATTTGGTGGCAACCCCATT+TGG 0.424538 5_1:+10184204 None:intergenic
CCTTCTGTAGCATAAATTGT+TGG 0.426238 5_1:+10184951 None:intergenic
TTTGCATTAGGAAGTGTTGT+AGG 0.428912 5_1:+10183561 None:intergenic
TATTTAGCAGGGTCTTTAGC+AGG 0.438959 5_1:+10183072 None:intergenic
TTTATTGTGTAACAGGAGAA+TGG 0.442017 5_1:-10184265 Msa0716710:intron
TCGGATGCACATACAATCAA+TGG 0.442094 5_1:-10184179 Msa0716710:CDS
GGAAGTGTTGTAGGAAATGA+AGG 0.446604 5_1:+10183570 None:intergenic
ACTTTGAGAGGAACATTAGC+AGG 0.448431 5_1:+10183477 None:intergenic
CAAGATCGCCTTTAATTCTA+CGG 0.451231 5_1:-10183202 Msa0716710:CDS
GTAGCATAAATTGTTGGCCC+TGG 0.454242 5_1:+10184957 None:intergenic
GCCCGTCTTAGTACCGAGAT+TGG 0.456234 5_1:+10183236 None:intergenic
CTAAACCAATAGTGTAGAAA+AGG 0.460863 5_1:+10183445 None:intergenic
CGGCTTTCGTTGTGGAAGAT+GGG 0.464431 5_1:-10182652 Msa0716710:CDS
ACTCAATGTCCAATCCAAAC+TGG 0.464665 5_1:-10184710 Msa0716710:CDS
TATCGCAATGGTTGGGCTGA+TGG 0.465845 5_1:-10184746 Msa0716710:CDS
AACCAATTGTAACAGTAAAT+GGG 0.467133 5_1:-10184986 Msa0716710:CDS
GTAAACCAAGGGAACCAAAT+GGG 0.470340 5_1:-10184218 Msa0716710:CDS
TTCGTTGTGGAAGATGGGCC+AGG 0.471809 5_1:-10182647 Msa0716710:CDS
TCAAATTCTCTAGCAGGTTG+TGG 0.473611 5_1:+10184551 None:intergenic
TAGCTTCCACCAGTTTGGAT+TGG 0.474223 5_1:+10184701 None:intergenic
GCAGGTTGTGGAAAAGGAAA+AGG 0.474816 5_1:+10184563 None:intergenic
TTGACCAGGACCAATTAGAA+TGG 0.476488 5_1:+10183737 None:intergenic
TTAGAGTTGCACACTGGTTG+GGG 0.479004 5_1:-10182683 Msa0716710:CDS
CAAAACAAAAGTTGTCTAAG+AGG 0.485707 5_1:-10185695 Msa0716710:five_prime_UTR
ACAAGTTCAAATTCTCTAGC+AGG 0.487160 5_1:+10184545 None:intergenic
TTGGACCGCAATTCGATTCA+CGG 0.487234 5_1:-10182998 Msa0716710:CDS
TCTTGTCCTACATGCATCAA+TGG 0.490940 5_1:-10183414 Msa0716710:CDS
GAACAAGGAAAGAGTGGTCC+TGG 0.497425 5_1:+10184152 None:intergenic
CGATTCACGGCTAATAATCC+AGG 0.504440 5_1:-10182985 Msa0716710:intron
AATATAACATATCAATTCAC+TGG 0.504449 5_1:-10184893 Msa0716710:intron
TTGGGGTCCCTACCGGTGGT+TGG 0.507604 5_1:-10183017 Msa0716710:CDS
CAGGTTGTGGAAAAGGAAAA+GGG 0.509861 5_1:+10184564 None:intergenic
AACACTGTTGGGGTCCCTAC+CGG 0.517718 5_1:-10183024 Msa0716710:CDS
TTTGGAAGGTCCTTTGGTGG+TGG 0.517886 5_1:+10182605 None:intergenic
TTTCATGGATGCACCACTCT+CGG 0.522361 5_1:-10183652 Msa0716710:CDS
AGTAAACCAAGGGAACCAAA+TGG 0.525185 5_1:-10184219 Msa0716710:CDS
CAGGACAAAGAGGAACTTTG+TGG 0.526013 5_1:-10184658 Msa0716710:CDS
TTAAACAGTATCGCAATGGT+TGG 0.526070 5_1:-10184754 Msa0716710:CDS
AGAATGGATTGTGTTGTGAA+TGG 0.527678 5_1:+10183753 None:intergenic
TCGAATTGCGGTCCAACCAC+CGG 0.530904 5_1:+10183005 None:intergenic
ATTTAGAGTTGCACACTGGT+TGG 0.533023 5_1:-10182685 Msa0716710:CDS
TTCTTACGTTTCTCGGAACA+AGG 0.533666 5_1:+10184137 None:intergenic
TAAACAGTATCGCAATGGTT+GGG 0.534891 5_1:-10184753 Msa0716710:CDS
GCCAATCTCGGTACTAAGAC+GGG 0.536336 5_1:-10183237 Msa0716710:CDS
AGAACATTTGTGGTTTGACC+AGG 0.539222 5_1:+10183723 None:intergenic
CGGATGCACATACAATCAAT+GGG 0.539224 5_1:-10184178 Msa0716710:CDS
AGAGAATTTGAACTTGTGCT+AGG 0.539942 5_1:-10184539 Msa0716710:intron
GCACCGTTGACCGCCAATCT+CGG 0.543409 5_1:-10183249 Msa0716710:CDS
ACAGTAAATGGGAGGTACCC+AGG 0.547259 5_1:-10184975 Msa0716710:CDS
CAATCCATTCTAATTGGTCC+TGG 0.548604 5_1:-10183741 Msa0716710:CDS
GGACATTGAGTAATATAAGC+TGG 0.548729 5_1:+10184722 None:intergenic
GTAGCAACAAAGTATCTGCT+TGG 0.549623 5_1:+10183681 None:intergenic
GTTGTCTAAGAGGAGCATAA+TGG 0.556107 5_1:-10185685 Msa0716710:exon
TATGCAATGGAGGTTGAGAA+AGG 0.556866 5_1:-10183885 Msa0716710:CDS
CATACAATCAATGGGAAGCC+AGG 0.558040 5_1:-10184170 Msa0716710:CDS
TAGCATAAATTGTTGGCCCT+GGG 0.558784 5_1:+10184958 None:intergenic
ATCTGTTCTACCACCACCAA+AGG 0.559203 5_1:-10182615 Msa0716710:CDS
ACATATGACTTCAATGTCAC+AGG 0.560816 5_1:-10184677 Msa0716710:CDS
TGTATGATTATATAGGTGTA+TGG 0.566550 5_1:-10182721 Msa0716710:intron
CGCCAATCTCGGTACTAAGA+CGG 0.571925 5_1:-10183238 Msa0716710:CDS
TGTCATGTTGTGACCAGCAA+GGG 0.572003 5_1:+10183806 None:intergenic
AAAGGACCTTCCAAAATGCT+AGG 0.572454 5_1:-10182597 Msa0716710:exon
TGAAGATACATATGCAATGG+AGG 0.572819 5_1:-10183895 Msa0716710:intron
CCTATGGAGAGGAACACTGT+TGG 0.575145 5_1:-10183036 Msa0716710:CDS
AGGTTTCTATCAACTTTGAG+AGG 0.579119 5_1:+10183465 None:intergenic
CAGTAAATGGGAGGTACCCA+GGG 0.579786 5_1:-10184974 Msa0716710:CDS
CAACCAAATTATATTTAGCA+GGG 0.585738 5_1:+10183061 None:intergenic
TTCAATGTCACAGGACAAAG+AGG 0.588439 5_1:-10184668 Msa0716710:CDS
GTACCGAGATTGGCGGTCAA+CGG 0.589957 5_1:+10183246 None:intergenic
GCTCATCACTTTGATATCAA+GGG 0.590306 5_1:-10183327 Msa0716710:CDS
ATTGAAAACGGCTTTCGTTG+TGG 0.592669 5_1:-10182660 Msa0716710:CDS
CCAACAGTGTTCCTCTCCAT+AGG 0.596019 5_1:+10183036 None:intergenic
CAATGTCCAATCCAAACTGG+TGG 0.606738 5_1:-10184707 Msa0716710:CDS
TTAACAAAGATATGTGACTT+CGG 0.608585 5_1:+10185643 None:intergenic
AGAATGGTGGCACAAAGATG+TGG 0.608690 5_1:-10184249 Msa0716710:CDS
ATTGTTATCATGCCTAAACC+AGG 0.611746 5_1:-10184590 Msa0716710:CDS
CCAACAATTTATGCTACAGA+AGG 0.615473 5_1:-10184951 Msa0716710:CDS
TTCTCGGAACAAGGAAAGAG+TGG 0.616470 5_1:+10184146 None:intergenic
TTGCGGTCCAACCACCGGTA+GGG 0.617520 5_1:+10183010 None:intergenic
CGTCTTAGTACCGAGATTGG+CGG 0.619297 5_1:+10183239 None:intergenic
GGGGTTGCCACCAAATATGT+CGG 0.620199 5_1:-10184198 Msa0716710:CDS
TAAACCAAGGGAACCAAATG+GGG 0.620759 5_1:-10184217 Msa0716710:CDS
ACAGCTATATTCCAATACAA+AGG 0.622211 5_1:-10183612 Msa0716710:CDS
CTGTCATGTTGTGACCAGCA+AGG 0.622828 5_1:+10183805 None:intergenic
TATGGAGAGGAACACTGTTG+GGG 0.624128 5_1:-10183034 Msa0716710:CDS
TGGAAGAGATAGTAAACCAA+GGG 0.624447 5_1:-10184229 Msa0716710:CDS
ACTGTTGGGGTCCCTACCGG+TGG 0.626035 5_1:-10183021 Msa0716710:CDS
CGTGTTCCATTGATGCATGT+AGG 0.626273 5_1:+10183408 None:intergenic
CTTTGAGAGGAACATTAGCA+GGG 0.634352 5_1:+10183478 None:intergenic
TGTCATTTAGAGTTGCACAC+TGG 0.638629 5_1:-10182689 Msa0716710:CDS
ATTGCGGTCCAACCACCGGT+AGG 0.642154 5_1:+10183009 None:intergenic
GGACTTAAACAGTATCGCAA+TGG 0.642768 5_1:-10184758 Msa0716710:CDS
GTATAGTTAAAAGGAGCCAA+AGG 0.642857 5_1:+10183276 None:intergenic
ATTAGCCGTGAATCGAATTG+CGG 0.656457 5_1:+10182993 None:intergenic
GTGATGAGCTTGAAGAAGCG+CGG 0.657612 5_1:+10183341 None:intergenic
ATTTGGTTGATCCTATGGAG+AGG 0.658256 5_1:-10183047 Msa0716710:CDS
GTGGAAGAGATAGTAAACCA+AGG 0.661655 5_1:-10184230 Msa0716710:CDS
GAAGTGTTGTAGGAAATGAA+GGG 0.669774 5_1:+10183571 None:intergenic
ATTGTGTAACAGGAGAATGG+TGG 0.675069 5_1:-10184262 Msa0716710:intron
GACAAAGAGGAACTTTGTGG+TGG 0.676053 5_1:-10184655 Msa0716710:CDS
AGGTTGTGGAAAAGGAAAAG+GGG 0.686432 5_1:+10184565 None:intergenic
TGTGCATCCGACATATTTGG+TGG 0.699108 5_1:+10184191 None:intergenic
CAATTGTAACAGTAAATGGG+AGG 0.721812 5_1:-10184983 Msa0716710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTATAATATATATAAGTA+TGG - chr5_1:10182537-10182556 Msa0716710:three_prime_UTR 10.0%
!! AAATAATGATAAAAAAACAT+GGG + chr5_1:10182636-10182655 None:intergenic 10.0%
!! AAAATAATGATAAAAAAACA+TGG + chr5_1:10182637-10182656 None:intergenic 10.0%
!!! ATTATTTTTTATACGATTTT+GGG - chr5_1:10182649-10182668 Msa0716710:CDS 10.0%
!!! GTTTTTGTTATATAAATTAT+AGG - chr5_1:10183286-10183305 Msa0716710:CDS 10.0%
!!! TTTTTTATTTTTGTTTCTAA+AGG - chr5_1:10185253-10185272 Msa0716710:intron 10.0%
!!! CATTATTTTTTATACGATTT+TGG - chr5_1:10182648-10182667 Msa0716710:CDS 15.0%
!! AAAAAATTGAATTTGTATCA+TGG + chr5_1:10182842-10182861 None:intergenic 15.0%
!!! CTTTAAAATAAATCACTTTT+AGG + chr5_1:10182979-10182998 None:intergenic 15.0%
!!! TTGATATGTTATATTTTACA+TGG + chr5_1:10183172-10183191 None:intergenic 15.0%
!!! TTTTTTACCAAACATAAATT+TGG - chr5_1:10183215-10183234 Msa0716710:CDS 15.0%
!!! GTGATTTAAAACTTTTATTT+AGG - chr5_1:10183727-10183746 Msa0716710:CDS 15.0%
!! TTCAAATAAACTAAATTAGA+AGG + chr5_1:10183753-10183772 None:intergenic 15.0%
!!! TTAATTAACTTAGTTTTGTT+TGG + chr5_1:10184106-10184125 None:intergenic 15.0%
!!! CAAATTACAAATTTTAATGA+GGG + chr5_1:10184151-10184170 None:intergenic 15.0%
!!! ACAAATTACAAATTTTAATG+AGG + chr5_1:10184152-10184171 None:intergenic 15.0%
!! TTAGAGATGAAAAAAAAAAT+TGG - chr5_1:10185156-10185175 Msa0716710:intron 15.0%
!! AAAATGAAAATCTTCTAAAT+TGG + chr5_1:10185185-10185204 None:intergenic 15.0%
!! ATTGTTATATTTATCTTTCT+GGG - chr5_1:10185703-10185722 Msa0716710:five_prime_UTR 15.0%
!!! TCTTTGTTAATAGTTCTTTT+TGG - chr5_1:10182440-10182459 Msa0716710:three_prime_UTR 20.0%
!!! TATGTTATATTTTACATGGT+TGG + chr5_1:10183168-10183187 None:intergenic 20.0%
!! AATATAACATATCAATTCAC+TGG - chr5_1:10183177-10183196 Msa0716710:CDS 20.0%
!! TTTGGTAAAAAAGAACAAAT+TGG + chr5_1:10183207-10183226 None:intergenic 20.0%
!! TGTTATATAAATTATAGGCA+TGG - chr5_1:10183291-10183310 Msa0716710:CDS 20.0%
!!! TTCAAATTTTATTGTGTAAC+AGG - chr5_1:10183798-10183817 Msa0716710:CDS 20.0%
!!! AATTTTAATGAGGGAAATTT+AGG + chr5_1:10184142-10184161 None:intergenic 20.0%
!! AAATTCTTACCATATGATAT+TGG - chr5_1:10185307-10185326 Msa0716710:intron 20.0%
!!! TTTTGCATGTATGATTATAT+AGG - chr5_1:10185342-10185361 Msa0716710:intron 20.0%
!! GATTGTTATATTTATCTTTC+TGG - chr5_1:10185702-10185721 Msa0716710:five_prime_UTR 20.0%
! TTAACAAAGATATGTGACTT+CGG + chr5_1:10182430-10182449 None:intergenic 25.0%
!!! ATAGTTCTTTTTGGTTTTCT+TGG - chr5_1:10182449-10182468 Msa0716710:three_prime_UTR 25.0%
!!! ATTATGTCACGTTTTAGTAA+AGG + chr5_1:10182611-10182630 None:intergenic 25.0%
! AGATGAGAATCTGAAAAAAT+AGG + chr5_1:10182923-10182942 None:intergenic 25.0%
! AAACCAATTGTAACAGTAAA+TGG - chr5_1:10183083-10183102 Msa0716710:CDS 25.0%
! AACCAATTGTAACAGTAAAT+GGG - chr5_1:10183084-10183103 Msa0716710:CDS 25.0%
! TATTTGCAACTAAAAAAGTG+CGG - chr5_1:10183582-10183601 Msa0716710:CDS 25.0%
! ATAATTAGTTGAACGAGATA+AGG + chr5_1:10184080-10184099 None:intergenic 25.0%
! TTATCTCGTTCAACTAATTA+TGG - chr5_1:10184079-10184098 Msa0716710:intron 25.0%
!!! TTTTGAAGATACATATGCAA+TGG - chr5_1:10184172-10184191 Msa0716710:CDS 25.0%
! TTATAACCATGAAGATGAAA+TGG + chr5_1:10184941-10184960 None:intergenic 25.0%
!!! GGTTATAACTTTTTTGTTGT+TGG - chr5_1:10184953-10184972 Msa0716710:CDS 25.0%
! CAACCAAATTATATTTAGCA+GGG + chr5_1:10185012-10185031 None:intergenic 25.0%
! TCAACCAAATTATATTTAGC+AGG + chr5_1:10185013-10185032 None:intergenic 25.0%
! ATATAATTTGGTTGATCCTA+TGG - chr5_1:10185018-10185037 Msa0716710:CDS 25.0%
!! TTTACGTCTCTAATTTTCTT+CGG - chr5_1:10185204-10185223 Msa0716710:intron 25.0%
! TGTATGATTATATAGGTGTA+TGG - chr5_1:10185349-10185368 Msa0716710:intron 25.0%
! TTAACCAAAAAGAAATTGCT+AGG - chr5_1:10185501-10185520 Msa0716710:intron 25.0%
CAAAACAAAAGTTGTCTAAG+AGG - chr5_1:10182375-10182394 Msa0716710:three_prime_UTR 30.0%
GCATATAACTCACATCAAAT+TGG + chr5_1:10182514-10182533 None:intergenic 30.0%
TAACATATCAATTCACTGGT+AGG - chr5_1:10183181-10183200 Msa0716710:CDS 30.0%
CATGTGACCAAATTTATGTT+TGG + chr5_1:10183225-10183244 None:intergenic 30.0%
GAGATTCTAACAAGAAAACA+TGG - chr5_1:10183257-10183276 Msa0716710:CDS 30.0%
ATGCTCATATTCTTTGGTTA+AGG - chr5_1:10183439-10183458 Msa0716710:CDS 30.0%
TGCTCATATTCTTTGGTTAA+GGG - chr5_1:10183440-10183459 Msa0716710:CDS 30.0%
ATACAATACAACCTCAATCA+TGG + chr5_1:10183650-10183669 None:intergenic 30.0%
TGATTGAGGTTGTATTGTAT+CGG - chr5_1:10183650-10183669 Msa0716710:CDS 30.0%
TTTATTGTGTAACAGGAGAA+TGG - chr5_1:10183805-10183824 Msa0716710:CDS 30.0%
GTTATTGTTCTTACGTTTCT+CGG + chr5_1:10183943-10183962 None:intergenic 30.0%
AGGTCGCTTATAAAAAATGA+CGG + chr5_1:10183983-10184002 None:intergenic 30.0%
ACAACACAATCCATTCTAAT+TGG - chr5_1:10184323-10184342 Msa0716710:intron 30.0%
ACAGCTATATTCCAATACAA+AGG - chr5_1:10184458-10184477 Msa0716710:intron 30.0%
! TGTTTGGAATTCCTTTGTAT+TGG + chr5_1:10184472-10184491 None:intergenic 30.0%
!! AAGTTTGTGTCATTTGCATT+AGG + chr5_1:10184524-10184543 None:intergenic 30.0%
! AGAAACCTTTTCTACACTAT+TGG - chr5_1:10184620-10184639 Msa0716710:CDS 30.0%
CTAAACCAATAGTGTAGAAA+AGG + chr5_1:10184628-10184647 None:intergenic 30.0%
!!! CTTTTCTACACTATTGGTTT+AGG - chr5_1:10184626-10184645 Msa0716710:CDS 30.0%
!!! AACTTTTTTGTTGTTGGAAC+TGG - chr5_1:10184959-10184978 Msa0716710:CDS 30.0%
AGACCCTGCTAAATATAATT+TGG - chr5_1:10185006-10185025 Msa0716710:CDS 30.0%
! TTTTAATGCAGTTGTTGACA+AGG + chr5_1:10185115-10185134 None:intergenic 30.0%
TGTCAACAACTGCATTAAAA+AGG - chr5_1:10185115-10185134 Msa0716710:intron 30.0%
TCATATGGTAAGAATTTGAC+AGG + chr5_1:10185304-10185323 None:intergenic 30.0%
! AGAATAACTGCAATTTTTGC+AGG + chr5_1:10185613-10185632 None:intergenic 30.0%
! TTCTTTATTGCTGCTTCAAC+AGG + chr5_1:10182488-10182507 None:intergenic 35.0%
!!! CTGTTACAATTGGTTTTGCA+TGG + chr5_1:10183079-10183098 None:intergenic 35.0%
CTCCCATTTACTGTTACAAT+TGG + chr5_1:10183089-10183108 None:intergenic 35.0%
CAATTGTAACAGTAAATGGG+AGG - chr5_1:10183087-10183106 Msa0716710:CDS 35.0%
CCTTCTGTAGCATAAATTGT+TGG + chr5_1:10183122-10183141 None:intergenic 35.0%
CCAACAATTTATGCTACAGA+AGG - chr5_1:10183119-10183138 Msa0716710:CDS 35.0%
TTAAACAGTATCGCAATGGT+TGG - chr5_1:10183316-10183335 Msa0716710:CDS 35.0%
TAAACAGTATCGCAATGGTT+GGG - chr5_1:10183317-10183336 Msa0716710:CDS 35.0%
GGACATTGAGTAATATAAGC+TGG + chr5_1:10183351-10183370 None:intergenic 35.0%
ACATATGACTTCAATGTCAC+AGG - chr5_1:10183393-10183412 Msa0716710:CDS 35.0%
ATTGTTATCATGCCTAAACC+AGG - chr5_1:10183480-10183499 Msa0716710:CDS 35.0%
ACAAGTTCAAATTCTCTAGC+AGG + chr5_1:10183528-10183547 None:intergenic 35.0%
AGAGAATTTGAACTTGTGCT+AGG - chr5_1:10183531-10183550 Msa0716710:CDS 35.0%
TGGAAGAGATAGTAAACCAA+GGG - chr5_1:10183841-10183860 Msa0716710:CDS 35.0%
TCGCTTATAAAAAATGACGG+AGG + chr5_1:10183980-10183999 None:intergenic 35.0%
CAAAAGAACGCTTATAACGA+GGG - chr5_1:10184013-10184032 Msa0716710:intron 35.0%
TGAAGATACATATGCAATGG+AGG - chr5_1:10184175-10184194 Msa0716710:CDS 35.0%
!! AGAATGGATTGTGTTGTGAA+TGG + chr5_1:10184320-10184339 None:intergenic 35.0%
TGCTTTGACTAGAACATTTG+TGG + chr5_1:10184360-10184379 None:intergenic 35.0%
TGTTGCTACAAGAACTTTCA+TGG - chr5_1:10184403-10184422 Msa0716710:intron 35.0%
! AATGAAGGGATGATAGTGTT+TGG + chr5_1:10184488-10184507 None:intergenic 35.0%
!! GAAGTGTTGTAGGAAATGAA+GGG + chr5_1:10184502-10184521 None:intergenic 35.0%
!! TTTGCATTAGGAAGTGTTGT+AGG + chr5_1:10184512-10184531 None:intergenic 35.0%
AGGTTTCTATCAACTTTGAG+AGG + chr5_1:10184608-10184627 None:intergenic 35.0%
AGCTCATCACTTTGATATCA+AGG - chr5_1:10184742-10184761 Msa0716710:CDS 35.0%
GCTCATCACTTTGATATCAA+GGG - chr5_1:10184743-10184762 Msa0716710:CDS 35.0%
! TGATTTTCCTGATAAGCCTT+TGG - chr5_1:10184778-10184797 Msa0716710:intron 35.0%
GTATAGTTAAAAGGAGCCAA+AGG + chr5_1:10184797-10184816 None:intergenic 35.0%
!! TTGGCTCCTTTTAACTATAC+TGG - chr5_1:10184797-10184816 Msa0716710:intron 35.0%
CAAGATCGCCTTTAATTCTA+CGG - chr5_1:10184868-10184887 Msa0716710:intron 35.0%
TCACATCCATTTCATCTTCA+TGG - chr5_1:10184932-10184951 Msa0716710:CDS 35.0%
!! TTTGTTGTTGGAACTGGAAT+TGG - chr5_1:10184965-10184984 Msa0716710:CDS 35.0%
GAATTTGACAGGACAATGTA+AGG + chr5_1:10185293-10185312 None:intergenic 35.0%
ACATGCATGCCAATATCATA+TGG + chr5_1:10185319-10185338 None:intergenic 35.0%
! AGAACAGATTGATCTTTTCC+TGG + chr5_1:10185444-10185463 None:intergenic 35.0%
! GTTAAACTTGCCTAGCATTT+TGG + chr5_1:10185486-10185505 None:intergenic 35.0%
! CATGCCTAGCAATTTCTTTT+TGG + chr5_1:10185508-10185527 None:intergenic 35.0%
CAAAAAGAAATTGCTAGGCA+TGG - chr5_1:10185506-10185525 Msa0716710:intron 35.0%
GTCTCATAGTTCAGATTGTA+TGG - chr5_1:10185537-10185556 Msa0716710:intron 35.0%
! GTTGTCTAAGAGGAGCATAA+TGG - chr5_1:10182385-10182404 Msa0716710:three_prime_UTR 40.0%
TAGCATAAATTGTTGGCCCT+GGG + chr5_1:10183115-10183134 None:intergenic 40.0%
GGACTTAAACAGTATCGCAA+TGG - chr5_1:10183312-10183331 Msa0716710:CDS 40.0%
ACTCAATGTCCAATCCAAAC+TGG - chr5_1:10183360-10183379 Msa0716710:CDS 40.0%
ATGTATAGCTTCCACCAGTT+TGG + chr5_1:10183377-10183396 None:intergenic 40.0%
TTCAATGTCACAGGACAAAG+AGG - chr5_1:10183402-10183421 Msa0716710:CDS 40.0%
TGGTTAAGGGCTACTGTTTA+TGG - chr5_1:10183453-10183472 Msa0716710:CDS 40.0%
AGGTTGTGGAAAAGGAAAAG+GGG + chr5_1:10183508-10183527 None:intergenic 40.0%
CAGGTTGTGGAAAAGGAAAA+GGG + chr5_1:10183509-10183528 None:intergenic 40.0%
TCAAATTCTCTAGCAGGTTG+TGG + chr5_1:10183522-10183541 None:intergenic 40.0%
TACAACCTCAATCATGGTCA+TGG + chr5_1:10183644-10183663 None:intergenic 40.0%
ATTGTGTAACAGGAGAATGG+TGG - chr5_1:10183808-10183827 Msa0716710:CDS 40.0%
GTGGAAGAGATAGTAAACCA+AGG - chr5_1:10183840-10183859 Msa0716710:CDS 40.0%
AGTAAACCAAGGGAACCAAA+TGG - chr5_1:10183851-10183870 Msa0716710:CDS 40.0%
GTAAACCAAGGGAACCAAAT+GGG - chr5_1:10183852-10183871 Msa0716710:CDS 40.0%
TAAACCAAGGGAACCAAATG+GGG - chr5_1:10183853-10183872 Msa0716710:CDS 40.0%
GTATGTGCATCCGACATATT+TGG + chr5_1:10183885-10183904 None:intergenic 40.0%
TCGGATGCACATACAATCAA+TGG - chr5_1:10183891-10183910 Msa0716710:CDS 40.0%
CGGATGCACATACAATCAAT+GGG - chr5_1:10183892-10183911 Msa0716710:CDS 40.0%
TTCTTACGTTTCTCGGAACA+AGG + chr5_1:10183936-10183955 None:intergenic 40.0%
! CGTTCTTTTGCACTCACAAA+AGG + chr5_1:10184003-10184022 None:intergenic 40.0%
GCAAAAGAACGCTTATAACG+AGG - chr5_1:10184012-10184031 Msa0716710:intron 40.0%
TATGCAATGGAGGTTGAGAA+AGG - chr5_1:10184185-10184204 Msa0716710:CDS 40.0%
! GAACTTTTCTTTTCCCTTGC+TGG - chr5_1:10184251-10184270 Msa0716710:CDS 40.0%
CAATCCATTCTAATTGGTCC+TGG - chr5_1:10184329-10184348 Msa0716710:intron 40.0%
TTGACCAGGACCAATTAGAA+TGG + chr5_1:10184336-10184355 None:intergenic 40.0%
AGAACATTTGTGGTTTGACC+AGG + chr5_1:10184350-10184369 None:intergenic 40.0%
! GTAGCAACAAAGTATCTGCT+TGG + chr5_1:10184392-10184411 None:intergenic 40.0%
!! GTTTTGTTATCAACCGAGAG+TGG + chr5_1:10184434-10184453 None:intergenic 40.0%
!! GGAAGTGTTGTAGGAAATGA+AGG + chr5_1:10184503-10184522 None:intergenic 40.0%
! CTTTGAGAGGAACATTAGCA+GGG + chr5_1:10184595-10184614 None:intergenic 40.0%
! ACTTTGAGAGGAACATTAGC+AGG + chr5_1:10184596-10184615 None:intergenic 40.0%
TCTTGTCCTACATGCATCAA+TGG - chr5_1:10184656-10184675 Msa0716710:CDS 40.0%
CAGCTCAACCGTAGAATTAA+AGG + chr5_1:10184879-10184898 None:intergenic 40.0%
CTTTAATTCTACGGTTGAGC+TGG - chr5_1:10184877-10184896 Msa0716710:intron 40.0%
TATTTAGCAGGGTCTTTAGC+AGG + chr5_1:10185001-10185020 None:intergenic 40.0%
ATTTGGTTGATCCTATGGAG+AGG - chr5_1:10185023-10185042 Msa0716710:CDS 40.0%
ATTAGCCGTGAATCGAATTG+CGG + chr5_1:10185080-10185099 None:intergenic 40.0%
AGTTGTTGACAAGGTATACC+TGG + chr5_1:10185106-10185125 None:intergenic 40.0%
! TGTCATTTAGAGTTGCACAC+TGG - chr5_1:10185381-10185400 Msa0716710:intron 40.0%
! ATTTAGAGTTGCACACTGGT+TGG - chr5_1:10185385-10185404 Msa0716710:intron 40.0%
! TTTAGAGTTGCACACTGGTT+GGG - chr5_1:10185386-10185405 Msa0716710:intron 40.0%
ATTGAAAACGGCTTTCGTTG+TGG - chr5_1:10185410-10185429 Msa0716710:intron 40.0%
!! TAGCATTTTGGAAGGTCCTT+TGG + chr5_1:10185474-10185493 None:intergenic 40.0%
AAAGGACCTTCCAAAATGCT+AGG - chr5_1:10185473-10185492 Msa0716710:intron 40.0%
! AACTTGCCTAGCATTTTGGA+AGG + chr5_1:10185482-10185501 None:intergenic 40.0%
GTAGCATAAATTGTTGGCCC+TGG + chr5_1:10183116-10183135 None:intergenic 45.0%
CAATGTCCAATCCAAACTGG+TGG - chr5_1:10183363-10183382 Msa0716710:CDS 45.0%
TAGCTTCCACCAGTTTGGAT+TGG + chr5_1:10183372-10183391 None:intergenic 45.0%
CAGGACAAAGAGGAACTTTG+TGG - chr5_1:10183412-10183431 Msa0716710:CDS 45.0%
GACAAAGAGGAACTTTGTGG+TGG - chr5_1:10183415-10183434 Msa0716710:CDS 45.0%
GGTGGCATGCTCATATTCTT+TGG - chr5_1:10183433-10183452 Msa0716710:CDS 45.0%
GCAGGTTGTGGAAAAGGAAA+AGG + chr5_1:10183510-10183529 None:intergenic 45.0%
TCTCTAGCAGGTTGTGGAAA+AGG + chr5_1:10183516-10183535 None:intergenic 45.0%
GATCTCCATGACCATGATTG+AGG - chr5_1:10183636-10183655 Msa0716710:CDS 45.0%
AGAATGGTGGCACAAAGATG+TGG - chr5_1:10183821-10183840 Msa0716710:CDS 45.0%
! TATTTGGTGGCAACCCCATT+TGG + chr5_1:10183869-10183888 None:intergenic 45.0%
TGTGCATCCGACATATTTGG+TGG + chr5_1:10183882-10183901 None:intergenic 45.0%
CATACAATCAATGGGAAGCC+AGG - chr5_1:10183900-10183919 Msa0716710:intron 45.0%
TTCTCGGAACAAGGAAAGAG+TGG + chr5_1:10183927-10183946 None:intergenic 45.0%
GACGGAGGTAGTACAAACTT+GGG + chr5_1:10183965-10183984 None:intergenic 45.0%
TGACGGAGGTAGTACAAACT+TGG + chr5_1:10183966-10183985 None:intergenic 45.0%
TGTCATGTTGTGACCAGCAA+GGG + chr5_1:10184267-10184286 None:intergenic 45.0%
TTTCATGGATGCACCACTCT+CGG - chr5_1:10184418-10184437 Msa0716710:intron 45.0%
CGTGTTCCATTGATGCATGT+AGG + chr5_1:10184665-10184684 None:intergenic 45.0%
AAAGGAGCCAAAGGCTTATC+AGG + chr5_1:10184788-10184807 None:intergenic 45.0%
GGTGCGCCAGTATAGTTAAA+AGG + chr5_1:10184806-10184825 None:intergenic 45.0%
CTATGGAGAGGAACACTGTT+GGG - chr5_1:10185035-10185054 Msa0716710:intron 45.0%
TATGGAGAGGAACACTGTTG+GGG - chr5_1:10185036-10185055 Msa0716710:intron 45.0%
TTGGACCGCAATTCGATTCA+CGG - chr5_1:10185072-10185091 Msa0716710:intron 45.0%
CGATTCACGGCTAATAATCC+AGG - chr5_1:10185085-10185104 Msa0716710:intron 45.0%
! TTAGAGTTGCACACTGGTTG+GGG - chr5_1:10185387-10185406 Msa0716710:intron 45.0%
! CACTGGTTGGGGATTGAAAA+CGG - chr5_1:10185398-10185417 Msa0716710:intron 45.0%
ATCTGTTCTACCACCACCAA+AGG - chr5_1:10185455-10185474 Msa0716710:intron 45.0%
!! CATTTTGGAAGGTCCTTTGG+TGG + chr5_1:10185471-10185490 None:intergenic 45.0%
!! ACAGTAAATGGGAGGTACCC+AGG - chr5_1:10183095-10183114 Msa0716710:CDS 50.0%
!! CAGTAAATGGGAGGTACCCA+GGG - chr5_1:10183096-10183115 Msa0716710:CDS 50.0%
TATCGCAATGGTTGGGCTGA+TGG - chr5_1:10183324-10183343 Msa0716710:CDS 50.0%
GGAAAAGGGGTTCCTGGTTT+AGG + chr5_1:10183495-10183514 None:intergenic 50.0%
GGAAAAGGAAAAGGGGTTCC+TGG + chr5_1:10183501-10183520 None:intergenic 50.0%
GGGGTTGCCACCAAATATGT+CGG - chr5_1:10183872-10183891 Msa0716710:CDS 50.0%
GAACAAGGAAAGAGTGGTCC+TGG + chr5_1:10183921-10183940 None:intergenic 50.0%
CTGTCATGTTGTGACCAGCA+AGG + chr5_1:10184268-10184287 None:intergenic 50.0%
!! GCTTGAAGAAGCGCGGTTTT+CGG + chr5_1:10184725-10184744 None:intergenic 50.0%
GTGATGAGCTTGAAGAAGCG+CGG + chr5_1:10184732-10184751 None:intergenic 50.0%
! CGTCTTAGTACCGAGATTGG+CGG + chr5_1:10184834-10184853 None:intergenic 50.0%
CGCCAATCTCGGTACTAAGA+CGG - chr5_1:10184832-10184851 Msa0716710:intron 50.0%
GCCAATCTCGGTACTAAGAC+GGG - chr5_1:10184833-10184852 Msa0716710:intron 50.0%
CCAACAGTGTTCCTCTCCAT+AGG + chr5_1:10185037-10185056 None:intergenic 50.0%
CCTATGGAGAGGAACACTGT+TGG - chr5_1:10185034-10185053 Msa0716710:intron 50.0%
ACGGCTTTCGTTGTGGAAGA+TGG - chr5_1:10185417-10185436 Msa0716710:intron 50.0%
CGGCTTTCGTTGTGGAAGAT+GGG - chr5_1:10185418-10185437 Msa0716710:intron 50.0%
TTTGGAAGGTCCTTTGGTGG+TGG + chr5_1:10185468-10185487 None:intergenic 50.0%
! GCAACCCCATTTGGTTCCCT+TGG + chr5_1:10183860-10183879 None:intergenic 55.0%
GTACCGAGATTGGCGGTCAA+CGG + chr5_1:10184827-10184846 None:intergenic 55.0%
! GCCCGTCTTAGTACCGAGAT+TGG + chr5_1:10184837-10184856 None:intergenic 55.0%
AACACTGTTGGGGTCCCTAC+CGG - chr5_1:10185046-10185065 Msa0716710:intron 55.0%
TCGAATTGCGGTCCAACCAC+CGG + chr5_1:10185068-10185087 None:intergenic 55.0%
TTCGTTGTGGAAGATGGGCC+AGG - chr5_1:10185423-10185442 Msa0716710:intron 55.0%
GCACCGTTGACCGCCAATCT+CGG - chr5_1:10184821-10184840 Msa0716710:intron 60.0%
TTGCGGTCCAACCACCGGTA+GGG + chr5_1:10185063-10185082 None:intergenic 60.0%
ATTGCGGTCCAACCACCGGT+AGG + chr5_1:10185064-10185083 None:intergenic 60.0%
ACTGTTGGGGTCCCTACCGG+TGG - chr5_1:10185049-10185068 Msa0716710:intron 65.0%
TTGGGGTCCCTACCGGTGGT+TGG - chr5_1:10185053-10185072 Msa0716710:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 10182343 10185749 10182343 ID=Msa0716710;Name=Msa0716710
chr5_1 mRNA 10182343 10185749 10182343 ID=Msa0716710-mRNA-1;Parent=Msa0716710;Name=Msa0716710-mRNA-1;_AED=0.29;_eAED=0.29;_QI=61|0.8|0.83|1|1|1|6|255|572
chr5_1 exon 10182343 10182728 10182343 ID=Msa0716710-mRNA-1:exon:4110;Parent=Msa0716710-mRNA-1
chr5_1 exon 10182986 10183912 10182986 ID=Msa0716710-mRNA-1:exon:4109;Parent=Msa0716710-mRNA-1
chr5_1 exon 10184126 10184272 10184126 ID=Msa0716710-mRNA-1:exon:4108;Parent=Msa0716710-mRNA-1
chr5_1 exon 10184540 10184784 10184540 ID=Msa0716710-mRNA-1:exon:4107;Parent=Msa0716710-mRNA-1
chr5_1 exon 10184894 10185045 10184894 ID=Msa0716710-mRNA-1:exon:4106;Parent=Msa0716710-mRNA-1
chr5_1 exon 10185572 10185749 10185572 ID=Msa0716710-mRNA-1:exon:4105;Parent=Msa0716710-mRNA-1
chr5_1 five_prime_UTR 10185689 10185749 10185689 ID=Msa0716710-mRNA-1:five_prime_utr;Parent=Msa0716710-mRNA-1
chr5_1 CDS 10185572 10185688 10185572 ID=Msa0716710-mRNA-1:cds;Parent=Msa0716710-mRNA-1
chr5_1 CDS 10184894 10185045 10184894 ID=Msa0716710-mRNA-1:cds;Parent=Msa0716710-mRNA-1
chr5_1 CDS 10184540 10184784 10184540 ID=Msa0716710-mRNA-1:cds;Parent=Msa0716710-mRNA-1
chr5_1 CDS 10184126 10184272 10184126 ID=Msa0716710-mRNA-1:cds;Parent=Msa0716710-mRNA-1
chr5_1 CDS 10182986 10183912 10182986 ID=Msa0716710-mRNA-1:cds;Parent=Msa0716710-mRNA-1
chr5_1 CDS 10182598 10182728 10182598 ID=Msa0716710-mRNA-1:cds;Parent=Msa0716710-mRNA-1
chr5_1 three_prime_UTR 10182343 10182597 10182343 ID=Msa0716710-mRNA-1:three_prime_utr;Parent=Msa0716710-mRNA-1
Gene Sequence

>Msa0716710

ATGGCTTATCATATGATGAGCTTCCGAAGTCACATATCTTTGTTAATAGTTCTTTTTGGTTTTCTTGGCTTAATCTCTTTTCCTGTTGAAGCAGCAATAAAGAAATACCAATTTGATATTCAAATGAAGAATGTGAGTAGACTATGCCATGCAAAACCAATTGTAACAGTAAATGGGAGGTACCCAGGGCCAACAATTTATGCTACAGAAGGAGATAGAGTACAAATCAATGTCACCAACCATGTAAAATATAACATATCAATTCACTGGCATGGACTTAAACAGTATCGCAATGGTTGGGCTGATGGACCAGCTTATATTACTCAATGTCCAATCCAAACTGGTGGAAGCTATACATATGACTTCAATGTCACAGGACAAAGAGGAACTTTGTGGTGGCATGCTCATATTCTTTGGTTAAGGGCTACTGTTTATGGTGCAATTGTTATCATGCCTAAACCAGGAACCCCTTTTCCTTTTCCACAACCTGCTAGAGAATTTGAACTTGTGCTAGGAGAATGGTGGCACAAAGATGTGGAAGAGATAGTAAACCAAGGGAACCAAATGGGGTTGCCACCAAATATGTCGGATGCACATACAATCAATGGGAAGCCAGGACCACTCTTTCCTTGTTCCGAGAAACGTAAGAACAATAACCCAAATACATATGCAATGGAGGTTGAGAAAGGCAAGACATATCTGCTAAGAATCATCAATGCTGCTCTTAATGATGAACTTTTCTTTTCCCTTGCTGGTCACAACATGACAGTAGTTGAAGTTGATGCAGTTTACACAAAACCATTCACAACACAATCCATTCTAATTGGTCCTGGTCAAACCACAAATGTTCTAGTCAAAGCAAACAAAATTCCAAGCAGATACTTTGTTGCTACAAGAACTTTCATGGATGCACCACTCTCGGTTGATAACAAAACTGCTACAGCTATATTCCAATACAAAGGAATTCCAAACACTATCATCCCTTCATTTCCTACAACACTTCCTAATGCAAATGACACAAACTTTGCTTTGAATTATAGCAAAAAAATAAGAAGCTTAAACTCTGCTAAATACCCTGCTAATGTTCCTCTCAAAGTTGATAGAAACCTTTTCTACACTATTGGTTTAGGCATGAATTCTTGTCCTACATGCATCAATGGAACACGTTTAGTTGCATCACTTAACAATGTAACTTTTGTGATGCCGAAAACCGCGCTTCTTCAAGCTCATCACTTTGATATCAAGGGTGTTTTTAGAACTGATTTTCCTGATAAGCCTTTGGCTCCTTTTAACTATACTGGCGCACCGTTGACCGCCAATCTCGGTACTAAGACGGGCACAAGAGTTAGCAAGATCGCCTTTAATTCTACGGTTGAGCTGGTTTTGCAAGATACTAATCTTCTCACAGTTGAATCACATCCATTTCATCTTCATGGTTATAACTTTTTTGTTGTTGGAACTGGAATTGGCAATTTTGATCCTGCTAAAGACCCTGCTAAATATAATTTGGTTGATCCTATGGAGAGGAACACTGTTGGGGTCCCTACCGGTGGTTGGACCGCAATTCGATTCACGGCTAATAATCCAGGTGTATGGTTCATGCATTGTCATTTAGAGTTGCACACTGGTTGGGGATTGAAAACGGCTTTCGTTGTGGAAGATGGGCCAGGAAAAGATCAATCTGTTCTACCACCACCAAAGGACCTTCCAAAATGCTAG
Protein sequence

>Msa0716710

MAYHMMSFRSHISLLIVLFGFLGLISFPVEAAIKKYQFDIQMKNVSRLCHAKPIVTVNGRYPGPTIYATEGDRVQINVTNHVKYNISIHWHGLKQYRNGWADGPAYITQCPIQTGGSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKPGTPFPFPQPAREFELVLGEWWHKDVEEIVNQGNQMGLPPNMSDAHTINGKPGPLFPCSEKRKNNNPNTYAMEVEKGKTYLLRIINAALNDELFFSLAGHNMTVVEVDAVYTKPFTTQSILIGPGQTTNVLVKANKIPSRYFVATRTFMDAPLSVDNKTATAIFQYKGIPNTIIPSFPTTLPNANDTNFALNYSKKIRSLNSAKYPANVPLKVDRNLFYTIGLGMNSCPTCINGTRLVASLNNVTFVMPKTALLQAHHFDIKGVFRTDFPDKPLAPFNYTGAPLTANLGTKTGTRVSKIAFNSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFDPAKDPAKYNLVDPMERNTVGVPTGGWTAIRFTANNPGVWFMHCHLELHTGWGLKTAFVVEDGPGKDQSVLPPPKDLPKC*