AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0615680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0615680 MtrunA17_Chr4g0052071 89.333 75 6 1 1 75 1 73 1.07e-41 130
Msa0615680 MtrunA17_Chr4g0052051 86.667 75 9 1 1 75 1 74 4.72e-40 126
Msa0615680 MtrunA17_Chr5g0443071 47.368 76 31 3 3 75 17 86 2.52e-16 66.6
Msa0615680 MtrunA17_Chr1g0152631 43.478 69 35 1 7 75 5 69 2.00e-12 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTTGCCTGTTTCATTCA+TGG 0.294686 4_2:+65191271 Msa0615680:CDS
TAAGGTTGTCTCTGCCTTTG+AGG 0.398295 4_2:+65191307 Msa0615680:CDS
ATATCCTCCATGAATGAAAC+AGG 0.439443 4_2:-65191278 None:intergenic
AAAACGGAATGTCAATGGTT+GGG 0.508031 4_2:+65191153 None:intergenic
GTTGTTGAAGAGGCTGAAGA+TGG 0.526001 4_2:+65191187 Msa0615680:CDS
AACAAGAACAACAGCCTCAA+AGG 0.541344 4_2:-65191321 None:intergenic
AGAGAAGATCGTTGTTGAAG+AGG 0.543554 4_2:+65191177 Msa0615680:CDS
CATCATCATCAGTATTCAAG+TGG 0.550675 4_2:+65191240 Msa0615680:CDS
GGCTGAAGATGGAAGCATTG+AGG 0.600381 4_2:+65191198 Msa0615680:CDS
ACAAAGGTAAAAGAAACAGA+TGG 0.641605 4_2:-65191348 None:intergenic
AGAAGCTGCAACCACAACAA+AGG 0.643314 4_2:-65191364 None:intergenic
TTTGCCTGTTTCATTCATGG+AGG 0.660420 4_2:+65191274 Msa0615680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATGGAGGATATTGTTTTTA+AGG + chr4_2:65191289-65191308 Msa0615680:CDS 30.0%
ACAAAGGTAAAAGAAACAGA+TGG - chr4_2:65191351-65191370 None:intergenic 30.0%
! TGTTTCTTTTACCTTTGTTG+TGG + chr4_2:65191353-65191372 Msa0615680:CDS 30.0%
! CATTGAGGTTGAAGTTTTTG+TGG + chr4_2:65191213-65191232 Msa0615680:CDS 35.0%
CATCATCATCAGTATTCAAG+TGG + chr4_2:65191240-65191259 Msa0615680:CDS 35.0%
TACTTTGCCTGTTTCATTCA+TGG + chr4_2:65191271-65191290 Msa0615680:CDS 35.0%
ATATCCTCCATGAATGAAAC+AGG - chr4_2:65191281-65191300 None:intergenic 35.0%
AGAGAAGATCGTTGTTGAAG+AGG + chr4_2:65191177-65191196 Msa0615680:CDS 40.0%
TTTGCCTGTTTCATTCATGG+AGG + chr4_2:65191274-65191293 Msa0615680:CDS 40.0%
AACAAGAACAACAGCCTCAA+AGG - chr4_2:65191324-65191343 None:intergenic 40.0%
GTTGTTGAAGAGGCTGAAGA+TGG + chr4_2:65191187-65191206 Msa0615680:CDS 45.0%
TAAGGTTGTCTCTGCCTTTG+AGG + chr4_2:65191307-65191326 Msa0615680:CDS 45.0%
AGAAGCTGCAACCACAACAA+AGG - chr4_2:65191367-65191386 None:intergenic 45.0%
!! GGCTGAAGATGGAAGCATTG+AGG + chr4_2:65191198-65191217 Msa0615680:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 65191161 65191388 65191161 ID=Msa0615680;Name=Msa0615680
chr4_2 mRNA 65191161 65191388 65191161 ID=Msa0615680-mRNA-1;Parent=Msa0615680;Name=Msa0615680-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|75
chr4_2 exon 65191161 65191388 65191161 ID=Msa0615680-mRNA-1:exon:18440;Parent=Msa0615680-mRNA-1
chr4_2 CDS 65191161 65191388 65191161 ID=Msa0615680-mRNA-1:cds;Parent=Msa0615680-mRNA-1
Gene Sequence

>Msa0615680

ATGTCAATGGTTGGGAAGAGAAGATCGTTGTTGAAGAGGCTGAAGATGGAAGCATTGAGGTTGAAGTTTTTGTGGAAATCATCATCATCAGTATTCAAGTGGAACAGACTTACTTTGCCTGTTTCATTCATGGAGGATATTGTTTTTAAGGTTGTCTCTGCCTTTGAGGCTGTTGTTCTTGTTCTCACCATCTGTTTCTTTTACCTTTGTTGTGGTTGCAGCTTCTAA

Protein sequence

>Msa0615680

MSMVGKRRSLLKRLKMEALRLKFLWKSSSSVFKWNRLTLPVSFMEDIVFKVVSAFEAVVLVLTICFFYLCCGCSF*