AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0629580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0629580 MtrunA17_Chr4g0005901 97.920 577 11 1 1 576 1 577 0.0 1170
Msa0629580 MtrunA17_Chr7g0241741 84.670 561 86 0 12 572 3 563 0.0 1006
Msa0629580 MtrunA17_Chr3g0104011 70.213 564 166 2 10 571 1 564 0.0 827
Msa0629580 MtrunA17_Chr8g0375311 62.257 567 209 5 11 573 20 585 0.0 733
Msa0629580 MtrunA17_Chr8g0375291 61.649 558 209 5 11 564 20 576 0.0 716
Msa0629580 MtrunA17_Chr8g0375341 54.270 562 252 5 16 573 25 585 0.0 643
Msa0629580 MtrunA17_Chr8g0375351 54.093 562 253 5 16 573 25 585 0.0 640
Msa0629580 MtrunA17_Chr8g0375321 52.557 567 264 5 11 573 17 582 0.0 631
Msa0629580 MtrunA17_Chr8g0375331 52.669 562 261 5 16 573 9 569 0.0 615
Msa0629580 MtrunA17_Chr8g0375361 53.427 569 260 5 11 575 19 586 0.0 611
Msa0629580 MtrunA17_Chr3g0142101 47.687 562 280 6 6 564 10 560 0.0 528
Msa0629580 MtrunA17_Chr7g0261811 44.542 568 302 3 9 569 5 566 3.21e-179 519
Msa0629580 MtrunA17_Chr7g0261881 45.070 568 299 4 9 569 5 566 1.67e-177 514
Msa0629580 MtrunA17_Chr7g0261791 42.784 582 317 5 1 574 1 574 2.06e-174 507
Msa0629580 MtrunA17_Chr5g0422881 46.364 550 284 7 31 572 20 566 2.57e-172 501
Msa0629580 MtrunA17_Chr7g0261851 44.214 579 307 4 1 572 1 570 2.07e-171 499
Msa0629580 MtrunA17_Chr7g0261771 42.014 576 321 3 1 569 1 570 3.33e-170 496
Msa0629580 MtrunA17_Chr3g0114501 44.544 559 304 4 16 571 7 562 2.89e-169 493
Msa0629580 MtrunA17_Chr3g0142091 45.660 576 288 9 2 571 4 560 1.83e-168 491
Msa0629580 MtrunA17_Chr5g0399071 45.961 557 289 8 23 570 17 570 1.01e-163 479
Msa0629580 MtrunA17_Chr7g0217461 45.789 570 287 7 17 571 10 572 1.42e-163 479
Msa0629580 MtrunA17_Chr1g0187241 42.806 563 307 6 16 570 11 566 6.42e-163 477
Msa0629580 MtrunA17_Chr7g0255621 42.652 558 310 5 26 575 15 570 2.30e-162 476
Msa0629580 MtrunA17_Chr1g0166711 43.672 561 304 5 16 570 10 564 1.91e-159 468
Msa0629580 MtrunA17_Chr5g0399061 42.832 558 305 7 23 570 19 572 2.09e-156 460
Msa0629580 MtrunA17_Chr1g0166741 43.169 549 292 6 12 551 4 541 2.83e-156 460
Msa0629580 MtrunA17_Chr1g0213711 41.071 560 318 6 11 567 4 554 2.88e-156 460
Msa0629580 MtrunA17_Chr4g0056431 44.821 560 294 7 23 570 17 573 7.87e-156 459
Msa0629580 MtrunA17_Chr5g0400371 42.403 566 315 5 11 567 92 655 9.54e-156 461
Msa0629580 MtrunA17_Chr3g0077151 42.199 564 292 7 15 573 12 546 1.27e-155 457
Msa0629580 MtrunA17_Chr7g0255601 41.176 578 327 7 4 572 1 574 8.37e-155 457
Msa0629580 MtrunA17_Chr1g0166791 40.917 567 324 5 10 570 3 564 9.23e-154 454
Msa0629580 MtrunA17_Chr4g0054891 43.088 557 303 6 17 565 96 646 2.22e-153 455
Msa0629580 MtrunA17_Chr3g0112671 41.696 566 316 7 11 575 13 565 1.92e-151 447
Msa0629580 MtrunA17_Chr4g0008651 40.351 570 332 6 7 574 10 573 1.25e-150 446
Msa0629580 MtrunA17_Chr7g0214731 41.986 574 309 11 16 570 44 612 1.97e-149 444
Msa0629580 MtrunA17_Chr7g0214461 40.034 582 334 10 1 576 46 618 1.77e-148 441
Msa0629580 MtrunA17_Chr4g0014871 40.681 558 305 7 19 571 18 554 2.03e-148 439
Msa0629580 MtrunA17_Chr7g0240381 38.821 577 337 7 1 575 1 563 2.18e-146 434
Msa0629580 MtrunA17_Chr7g0218851 41.513 542 304 7 32 567 47 581 2.47e-146 435
Msa0629580 MtrunA17_Chr4g0014791 41.208 563 302 7 12 569 10 548 8.93e-146 432
Msa0629580 MtrunA17_Chr4g0014841 40.925 562 305 7 12 569 10 548 1.50e-145 431
Msa0629580 MtrunA17_Chr4g0014781 40.747 562 308 6 12 569 10 550 2.17e-145 431
Msa0629580 MtrunA17_Chr5g0415791 42.096 563 307 8 24 570 18 577 3.08e-145 432
Msa0629580 MtrunA17_Chr7g0255611 38.045 573 343 6 11 575 11 579 7.24e-145 431
Msa0629580 MtrunA17_Chr8g0353031 41.772 553 289 6 33 572 20 552 5.26e-143 425
Msa0629580 MtrunA17_Chr4g0014801 40.925 562 307 6 12 569 10 550 7.07e-143 424
Msa0629580 MtrunA17_Chr1g0166771 43.222 509 273 5 50 553 10 507 7.54e-143 424
Msa0629580 MtrunA17_Chr4g0063591 44.876 566 290 11 16 571 7 560 2.35e-141 421
Msa0629580 MtrunA17_Chr3g0112611 40.036 557 326 7 19 572 20 571 4.14e-140 419
Msa0629580 MtrunA17_Chr5g0439381 42.681 567 296 11 17 574 13 559 8.86e-138 412
Msa0629580 MtrunA17_Chr2g0284701 39.138 580 329 11 12 570 12 588 1.95e-131 397
Msa0629580 MtrunA17_Chr4g0059991 37.390 567 338 8 17 570 11 573 5.87e-131 395
Msa0629580 MtrunA17_Chr7g0271221 38.235 544 318 8 31 570 17 546 4.57e-130 392
Msa0629580 MtrunA17_Chr7g0261661 42.576 458 250 3 119 569 1 452 1.37e-129 388
Msa0629580 MtrunA17_Chr4g0011471 40.977 532 298 8 46 567 31 556 1.94e-129 390
Msa0629580 MtrunA17_Chr4g0059971 35.195 591 357 7 1 571 1 585 7.89e-128 387
Msa0629580 MtrunA17_Chr3g0133341 39.209 556 315 8 27 572 10 552 2.69e-125 380
Msa0629580 MtrunA17_Chr2g0276751 37.993 558 320 9 19 570 26 563 2.97e-125 380
Msa0629580 MtrunA17_Chr1g0193391 37.615 545 320 10 31 570 17 546 2.87e-123 376
Msa0629580 MtrunA17_Chr7g0261751 39.044 502 257 5 1 459 1 496 3.62e-123 373
Msa0629580 MtrunA17_Chr8g0362711 39.054 571 316 9 20 570 13 571 5.97e-123 374
Msa0629580 MtrunA17_Chr4g0059981 35.921 554 334 9 37 574 37 585 2.00e-120 368
Msa0629580 MtrunA17_Chr3g0082631 44.676 432 225 5 151 571 32 460 3.20e-120 363
Msa0629580 MtrunA17_Chr8g0362781 34.435 575 363 5 12 573 11 584 6.48e-120 367
Msa0629580 MtrunA17_Chr8g0379971 38.435 588 330 9 19 574 8 595 7.66e-119 364
Msa0629580 MtrunA17_Chr5g0415961 36.945 563 342 8 19 570 12 572 1.48e-118 363
Msa0629580 MtrunA17_Chr1g0166731 42.048 459 254 5 118 570 9 461 8.97e-118 357
Msa0629580 MtrunA17_Chr4g0011041 35.253 573 349 11 10 567 13 578 1.10e-114 353
Msa0629580 MtrunA17_Chr4g0014861 40.371 431 238 5 146 571 13 429 1.17e-114 348
Msa0629580 MtrunA17_Chr1g0147631 36.833 581 336 11 1 567 1 564 3.89e-113 349
Msa0629580 MtrunA17_Chr4g0069581 39.673 489 275 10 88 570 43 517 5.26e-110 340
Msa0629580 MtrunA17_Chr8g0390331 37.636 550 319 11 31 567 15 553 7.55e-110 340
Msa0629580 MtrunA17_Chr8g0362801 39.817 437 255 4 144 575 33 466 1.18e-109 336
Msa0629580 MtrunA17_Chr4g0060001 35.740 554 340 9 24 567 22 569 1.42e-109 340
Msa0629580 MtrunA17_Chr8g0379991 37.943 564 305 8 52 570 1 564 6.53e-109 338
Msa0629580 MtrunA17_Chr2g0331521 34.767 558 339 11 24 567 17 563 7.20e-107 333
Msa0629580 MtrunA17_Chr1g0157601 34.296 554 346 10 25 567 7 553 1.01e-106 332
Msa0629580 MtrunA17_Chr7g0240271 32.920 565 366 7 18 572 11 572 1.07e-104 327
Msa0629580 MtrunA17_Chr2g0331481 35.636 550 333 11 31 567 25 566 4.59e-103 324
Msa0629580 MtrunA17_Chr1g0157611 34.483 580 341 14 1 567 1 554 2.34e-102 320
Msa0629580 MtrunA17_Chr2g0331471 35.413 545 331 11 31 562 88 624 2.69e-102 324
Msa0629580 MtrunA17_Chr6g0451841 36.594 552 303 10 39 571 13 536 1.22e-100 315
Msa0629580 MtrunA17_Chr7g0240281 34.336 565 342 12 27 572 20 574 6.21e-100 315
Msa0629580 MtrunA17_Chr7g0261731 42.529 348 188 2 1 342 1 342 5.12e-99 305
Msa0629580 MtrunA17_Chr1g0177591 34.615 572 345 13 2 567 16 564 2.66e-98 310
Msa0629580 MtrunA17_Chr2g0331511 33.577 548 347 10 31 567 21 562 8.53e-98 310
Msa0629580 MtrunA17_Chr1g0157621 33.779 524 332 9 52 567 1 517 4.13e-94 299
Msa0629580 MtrunA17_Chr4g0003761 32.904 544 346 8 35 567 34 569 1.88e-92 296
Msa0629580 MtrunA17_Chr3g0142111 48.667 300 151 3 12 308 27 326 2.07e-92 287
Msa0629580 MtrunA17_Chr2g0331461 32.601 546 345 9 31 562 25 561 3.00e-89 288
Msa0629580 MtrunA17_Chr5g0415841 33.909 463 291 6 119 570 1 459 1.07e-85 274
Msa0629580 MtrunA17_Chr1g0177611 36.923 455 261 11 119 567 1 435 2.23e-82 265
Msa0629580 MtrunA17_Chr2g0325951 30.688 567 361 9 19 572 2 549 4.64e-82 267
Msa0629580 MtrunA17_Chr7g0235521 32.974 464 298 7 119 572 1 461 2.04e-81 263
Msa0629580 MtrunA17_Chr7g0240211 33.190 464 297 7 119 572 1 461 2.22e-81 263
Msa0629580 MtrunA17_Chr7g0240181 33.190 464 297 7 119 572 1 461 2.49e-81 263
Msa0629580 MtrunA17_Chr7g0240131 32.759 464 299 7 119 572 1 461 5.03e-80 259
Msa0629580 MtrunA17_Chr2g0325961 30.931 569 358 10 19 572 2 550 1.38e-79 260
Msa0629580 MtrunA17_Chr3g0142121 46.947 262 128 3 303 564 12 262 9.35e-79 250
Msa0629580 MtrunA17_Chr1g0177631 36.123 454 266 10 119 567 1 435 2.05e-77 251
Msa0629580 MtrunA17_Chr2g0326211 29.123 570 363 10 18 572 6 549 1.73e-74 247
Msa0629580 MtrunA17_Chr7g0240151 31.897 464 285 8 119 572 1 443 8.94e-74 243
Msa0629580 MtrunA17_Chr3g0112631 39.711 277 163 3 301 575 3 277 6.19e-67 219
Msa0629580 MtrunA17_Chr4g0060071 39.590 293 166 4 280 570 15 298 1.51e-66 219
Msa0629580 MtrunA17_Chr7g0234151 32.222 450 271 8 118 567 3 418 1.75e-64 217
Msa0629580 MtrunA17_Chr7g0234381 31.556 450 274 8 118 567 3 418 2.17e-62 211
Msa0629580 MtrunA17_Chr7g0234181 31.767 447 271 8 121 567 1 413 2.26e-62 211
Msa0629580 MtrunA17_Chr7g0261721 41.294 201 117 1 371 570 1 201 2.88e-49 171
Msa0629580 MtrunA17_Chr3g0112651 41.837 196 110 3 107 301 10 202 1.08e-41 149
Msa0629580 MtrunA17_Chr1g0147591 37.245 196 111 4 156 345 9 198 1.45e-35 132
Msa0629580 MtrunA17_Chr4g0060061 48.696 115 59 0 145 259 14 128 8.53e-32 119
Msa0629580 MtrunA17_Chr1g0166721 50.980 102 50 0 16 117 10 111 2.97e-30 115
Msa0629580 MtrunA17_Chr7g0261831 37.736 159 92 2 258 415 3 155 8.37e-30 116
Msa0629580 MtrunA17_Chr2g0326221 29.289 239 141 4 334 572 15 225 1.39e-27 110
Msa0629580 MtrunA17_Chr7g0261671 43.590 117 66 0 1 117 2 118 9.54e-27 105
Msa0629580 MtrunA17_Chr4g0014851 43.925 107 60 0 19 125 18 124 4.77e-23 95.9
Msa0629580 MtrunA17_Chr8g0362791 46.988 83 42 1 37 117 42 124 1.18e-16 77.0
Msa0629580 MtrunA17_Chr3g0112661 37.273 110 69 0 19 128 19 128 4.14e-16 75.5
Msa0629580 MtrunA17_Chr7g0240221 35.227 88 57 0 18 105 14 101 6.90e-15 71.2
Msa0629580 MtrunA17_Chr7g0240191 35.227 88 57 0 18 105 14 101 6.90e-15 71.2
Msa0629580 MtrunA17_Chr7g0240161 35.227 88 57 0 18 105 14 101 6.90e-15 71.2
Msa0629580 MtrunA17_Chr7g0240141 34.091 88 58 0 18 105 14 101 2.99e-14 69.7
Msa0629580 MtrunA17_Chr7g0261821 44.444 63 35 0 508 570 1 63 3.98e-14 67.4
Msa0629580 MtrunA17_Chr5g0415851 45.946 74 38 1 45 116 6 79 3.53e-13 65.9
Msa0629580 MtrunA17_Chr1g0147581 33.333 123 72 3 425 544 1 116 6.57e-13 65.5
Msa0629580 MtrunA17_Chr3g0082621 47.887 71 37 0 47 117 2 72 7.09e-13 64.7
Msa0629580 MtrunA17_Chr7g0235511 33.333 90 58 1 18 105 14 103 1.78e-12 64.7
Msa0629580 MtrunA17_Chr4g0060051 35.922 103 59 1 19 121 10 105 9.58e-12 62.8
Msa0629580 MtrunA17_Chr8g0382591 34.286 105 65 2 303 403 5 109 1.18e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0629580 AT3G54140.1 75.887 564 134 2 10 571 1 564 0.0 906
Msa0629580 AT3G54140.2 75.887 564 134 2 10 571 1 564 0.0 906
Msa0629580 AT5G01180.2 72.518 564 154 1 11 573 3 566 0.0 865
Msa0629580 AT5G01180.3 72.518 564 154 1 11 573 3 566 0.0 865
Msa0629580 AT5G01180.1 72.518 564 154 1 11 573 3 566 0.0 865
Msa0629580 AT2G02040.1 61.228 570 212 6 10 573 18 584 0.0 716
Msa0629580 AT1G62200.1 57.143 560 226 5 18 571 37 588 0.0 662
Msa0629580 AT1G62200.3 54.499 589 225 7 18 571 51 631 0.0 646
Msa0629580 AT1G62200.2 54.499 589 225 7 18 571 37 617 0.0 645
Msa0629580 AT2G02020.1 52.120 566 227 8 11 572 20 545 0.0 594
Msa0629580 AT2G02020.2 48.410 566 227 10 11 572 20 524 0.0 529
Msa0629580 AT1G68570.1 47.312 558 284 6 23 572 15 570 5.56e-167 488
Msa0629580 AT1G68570.2 48.134 536 270 5 45 572 5 540 6.73e-165 481
Msa0629580 AT2G40460.1 41.860 559 313 5 15 570 6 555 2.28e-159 468
Msa0629580 AT4G21680.1 43.563 567 304 8 17 571 12 574 4.45e-155 457
Msa0629580 AT5G46050.1 42.007 538 298 5 16 546 10 540 1.62e-152 450
Msa0629580 AT2G26690.1 44.938 563 291 8 10 564 1 552 3.26e-151 447
Msa0629580 AT5G19640.1 41.349 578 315 8 2 574 28 586 1.99e-150 446
Msa0629580 AT5G46040.1 41.459 562 315 5 16 570 10 564 1.12e-149 443
Msa0629580 AT1G32450.1 41.637 562 316 6 17 567 21 581 1.56e-148 441
Msa0629580 AT3G54450.1 40.290 551 306 7 30 572 9 544 5.27e-148 438
Msa0629580 AT1G12110.1 42.473 558 308 8 23 571 17 570 1.10e-147 438
Msa0629580 AT2G37900.1 41.623 579 320 8 1 572 1 568 6.00e-147 436
Msa0629580 AT5G13400.1 41.093 567 313 8 22 572 52 613 1.76e-146 436
Msa0629580 AT2G26690.2 45.644 528 268 8 45 564 3 519 3.04e-143 425
Msa0629580 AT3G53960.1 41.219 558 319 8 19 572 23 575 3.85e-143 427
Msa0629580 AT1G22540.1 40.592 574 316 7 4 572 1 554 3.11e-141 421
Msa0629580 AT1G69870.1 41.016 551 309 10 31 570 49 594 1.29e-139 419
Msa0629580 AT2G26690.3 45.648 517 262 8 56 564 2 507 1.87e-139 415
Msa0629580 AT1G72140.1 37.608 577 317 6 9 571 6 553 2.51e-136 408
Msa0629580 AT1G72130.1 38.721 563 295 10 17 571 14 534 3.50e-136 407
Msa0629580 AT3G21670.1 42.424 561 289 10 23 570 19 558 1.15e-135 407
Msa0629580 AT1G72125.1 41.219 558 304 8 22 572 18 558 1.59e-134 404
Msa0629580 AT1G59740.1 39.674 552 318 7 18 562 26 569 2.11e-133 402
Msa0629580 AT2G37900.2 42.886 499 272 7 76 572 2 489 7.69e-132 394
Msa0629580 AT1G69850.1 39.384 584 321 9 12 570 4 579 6.35e-131 395
Msa0629580 AT1G72120.1 39.223 566 317 6 16 575 13 557 1.48e-129 390
Msa0629580 AT1G27040.2 39.789 568 305 9 19 569 9 556 3.80e-128 387
Msa0629580 AT1G27040.1 39.789 568 305 9 19 569 13 560 4.34e-128 387
Msa0629580 AT1G22550.1 38.210 581 322 8 11 575 5 564 1.24e-127 386
Msa0629580 AT1G22570.1 38.569 573 318 9 16 575 14 565 1.05e-125 381
Msa0629580 AT1G27040.3 40.219 547 290 9 40 569 2 528 9.51e-125 377
Msa0629580 AT1G27080.1 37.590 556 312 10 31 570 12 548 1.18e-121 371
Msa0629580 AT1G18880.1 35.965 570 345 10 18 572 4 568 1.75e-120 368
Msa0629580 AT1G33440.1 37.255 561 327 9 18 568 23 568 2.47e-119 366
Msa0629580 AT1G52190.1 37.184 554 325 10 31 567 19 566 3.27e-119 365
Msa0629580 AT1G22570.2 38.716 545 301 8 44 575 4 528 1.31e-118 362
Msa0629580 AT5G62730.1 37.077 561 329 8 19 571 42 586 8.51e-116 356
Msa0629580 AT5G46050.2 41.149 435 242 5 119 546 1 428 1.43e-114 349
Msa0629580 AT3G16180.1 37.189 562 322 14 26 567 15 565 8.33e-113 348
Msa0629580 AT5G62730.2 37.523 541 314 8 39 571 3 527 1.27e-112 346
Msa0629580 AT5G14940.1 34.588 558 320 11 31 567 5 538 5.69e-110 340
Msa0629580 AT5G62680.1 34.629 566 348 9 23 570 31 592 6.98e-110 342
Msa0629580 AT1G27080.2 36.902 523 295 10 64 570 3 506 2.51e-109 338
Msa0629580 AT3G47960.1 35.053 562 349 9 22 570 50 608 1.05e-108 339
Msa0629580 AT1G72130.2 38.018 434 230 7 146 571 14 416 1.49e-105 324
Msa0629580 AT1G72130.3 38.018 434 230 7 146 571 14 416 1.49e-105 324
Msa0629580 AT5G28470.2 33.213 554 353 10 31 574 15 561 2.00e-104 326
Msa0629580 AT1G69860.1 34.501 571 347 10 11 574 3 553 4.43e-102 320
Msa0629580 AT5G28470.1 33.032 554 352 11 31 574 15 559 1.88e-101 318
Msa0629580 AT3G01350.1 32.920 565 332 12 30 567 4 548 9.27e-97 306
Msa0629580 AT3G45650.1 30.728 563 360 9 17 572 6 545 3.34e-92 294
Msa0629580 AT3G45660.1 31.818 528 330 9 52 572 40 544 1.37e-89 287
Msa0629580 AT3G45660.3 31.818 528 330 9 52 572 40 544 1.37e-89 287
Msa0629580 AT3G45660.2 31.818 528 330 9 52 572 61 565 3.44e-89 287
Msa0629580 AT3G45680.1 30.442 565 362 12 15 572 4 544 4.99e-88 283
Msa0629580 AT3G25260.1 31.703 552 333 8 18 567 6 515 1.28e-87 281
Msa0629580 AT3G45650.2 29.542 589 359 10 17 572 6 571 5.65e-87 281
Msa0629580 AT3G45720.1 31.048 525 332 11 55 572 41 542 2.35e-86 279
Msa0629580 AT3G25280.1 31.284 553 340 7 18 567 6 521 1.09e-81 265
Msa0629580 AT3G45710.1 28.649 555 368 10 25 572 13 546 1.91e-79 261
Msa0629580 AT3G45700.1 29.298 570 354 15 17 574 4 536 3.52e-79 259
Msa0629580 AT3G45690.1 27.949 551 331 13 26 572 13 501 1.81e-69 233
Msa0629580 AT2G38100.2 25.875 514 345 9 61 568 2 485 1.01e-48 177
Msa0629580 AT2G38100.1 25.875 514 345 9 61 568 18 501 3.08e-48 176
Msa0629580 AT2G38100.3 25.875 514 345 9 61 568 24 507 3.94e-48 176
Msa0629580 AT5G11570.1 38.211 246 130 8 42 278 22 254 7.86e-42 157
Msa0629580 AT5G11570.1 34.137 249 154 4 320 567 237 476 2.00e-36 142

Find 146 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTGTGTGTTCAAGTTTC+CGG 0.181797 4_3:+10438948 None:intergenic
GTCGGATGGGGATGGGGTTT+TGG 0.221483 4_3:-10439175 Msa0629580:CDS
ACACTCTAAGTGTCATCTTC+TGG 0.233776 4_3:-10438505 Msa0629580:CDS
TCAAGTAGAGAGCTATAAAA+AGG 0.259295 4_3:+10439374 None:intergenic
AAACCGTGTGTTTCATCTTT+TGG 0.259430 4_3:-10439328 Msa0629580:CDS
CAAGTATGCATCAGCTAAAA+AGG 0.280188 4_3:+10439693 None:intergenic
ACATTGGTTTGAAGAATATA+AGG 0.302893 4_3:-10440754 Msa0629580:intron
TTTCCCTGATTTCAGGTGTT+TGG 0.304831 4_3:-10438774 Msa0629580:intron
CGACTCTATCTAAGCAATTC+AGG 0.306879 4_3:+10437840 None:intergenic
GCAAATCAATTGTTCTACTA+TGG 0.324130 4_3:-10440267 Msa0629580:exon
AAGTTATTGTTGCAGCATTT+AGG 0.328500 4_3:-10439048 Msa0629580:CDS
CCATCCCTATGTCGATCTTC+TGG 0.340879 4_3:-10438295 Msa0629580:CDS
CTCGAAGACCGTTACGGACT+TGG 0.350415 4_3:-10439784 Msa0629580:CDS
TTGCTTCCTCGGTGTTGGTT+TGG 0.353446 4_3:-10439210 Msa0629580:CDS
TGACCCCTATTCATATTATC+TGG 0.356962 4_3:+10438062 None:intergenic
TGTATCTATTGATTGCTAAC+AGG 0.357064 4_3:-10437986 Msa0629580:CDS
GAAGTAAAACCAATTGAAAA+AGG 0.359554 4_3:+10439255 None:intergenic
CACTCTAAGTGTCATCTTCT+GGG 0.360635 4_3:-10438504 Msa0629580:CDS
CGTTCGTTTACTCCCCGTTT+GGG 0.363000 4_3:-10438654 Msa0629580:CDS
ACAAAAGTTACTACAAGAAA+TGG 0.370351 4_3:-10438101 Msa0629580:CDS
AAAGCGTGCCGCTTTATTCT+CGG 0.387682 4_3:-10440137 Msa0629580:intron
ATCCACACAGAAATATTTGC+CGG 0.392205 4_3:+10437796 None:intergenic
TACTTGATTGCACTTGGAAC+CGG 0.396847 4_3:-10439358 Msa0629580:CDS
TCGTTCGTTTACTCCCCGTT+TGG 0.403486 4_3:-10438655 Msa0629580:CDS
TGATTTCAGGTGTTTGGATA+AGG 0.407783 4_3:-10438768 Msa0629580:intron
TGCAACAGTATACAGTCAAA+TGG 0.413873 4_3:-10438615 Msa0629580:CDS
GGATTCCAGATAATATGAAT+AGG 0.414072 4_3:-10438067 Msa0629580:CDS
ACAAACACGCCGTTAATTTG+TGG 0.414688 4_3:-10440312 Msa0629580:five_prime_UTR
TTCACTCACAACTAATGCAT+TGG 0.422202 4_3:-10438159 Msa0629580:CDS
GGAAATGTTTGTATCTAAAC+CGG 0.425824 4_3:-10437815 Msa0629580:three_prime_UTR
GTTGCAGCATTTAGGAAACT+TGG 0.431571 4_3:-10439040 Msa0629580:CDS
ATCATCTCCATGATCGTGGC+AGG 0.437702 4_3:-10438377 Msa0629580:CDS
CGGTGCACTCATTGCTTCCT+CGG 0.439767 4_3:-10439221 Msa0629580:CDS
ACTTGACAAATCCTTGTAAG+AGG 0.442092 4_3:+10439067 None:intergenic
GATTCCAGATAATATGAATA+GGG 0.442981 4_3:-10438066 Msa0629580:CDS
TAAAGAAGAGACTTATCATC+TGG 0.447791 4_3:+10439007 None:intergenic
TGTGAAAGATTGGCTTACTA+TGG 0.447799 4_3:-10439832 Msa0629580:CDS
GGCTATCAGTGATGCCCAAA+CGG 0.448610 4_3:+10438640 None:intergenic
ATGAATGTCGGATGGGGATG+GGG 0.453174 4_3:-10439181 Msa0629580:CDS
GTGCAGATCCTCCATGGATT+CGG 0.453674 4_3:+10438707 None:intergenic
ACTCATTGCTTCCTCGGTGT+TGG 0.455639 4_3:-10439215 Msa0629580:CDS
TCTTCGCAACCACAAATTAA+CGG 0.456697 4_3:+10440303 None:intergenic
TAGAATCCCTGCCACGATCA+TGG 0.457853 4_3:+10438370 None:intergenic
TCACTCACAACTAATGCATT+GGG 0.458337 4_3:-10438158 Msa0629580:CDS
ACTCCTCAGGTTACTCATCT+AGG 0.459663 4_3:+10437922 None:intergenic
GCTATCAGTGATGCCCAAAC+GGG 0.459760 4_3:+10438641 None:intergenic
ATGCATACTTGGGAAGATAC+TGG 0.462100 4_3:-10439681 Msa0629580:CDS
TGACACTTAGAGTGTCGAAA+AGG 0.462376 4_3:+10438513 None:intergenic
GATGAATGTCGGATGGGGAT+GGG 0.465810 4_3:-10439182 Msa0629580:CDS
GCTGTGTGAATCCGTGTTCA+TGG 0.471308 4_3:+10438432 None:intergenic
CTATGGCATGAGTACAAACT+TGG 0.471382 4_3:-10439815 Msa0629580:CDS
TGCCACGATCATGGAGATGA+TGG 0.471563 4_3:+10438379 None:intergenic
TAATGAATGTTGTGAAAGAT+TGG 0.472976 4_3:-10439842 Msa0629580:CDS
AACTATCTCGAAGACCGTTA+CGG 0.475606 4_3:-10439790 Msa0629580:CDS
AGATGAATGTCGGATGGGGA+TGG 0.477086 4_3:-10439183 Msa0629580:CDS
AAGCCTTCATGTGATGCTAA+TGG 0.479194 4_3:-10439427 Msa0629580:CDS
CCTTTCGCAAGAAAATTTAC+CGG 0.479983 4_3:-10438455 Msa0629580:CDS
CAATGAGACGGTCGTAGACC+GGG 0.481366 4_3:+10438480 None:intergenic
ACTACAAGAAATGGAAGTCT+TGG 0.484536 4_3:-10438092 Msa0629580:CDS
AATGGCTGCCATCCGACGTC+AGG 0.484612 4_3:-10439409 Msa0629580:CDS
TTCACACAGCTTCAAAGAAT+CGG 0.484967 4_3:-10438419 Msa0629580:CDS
ACAATGAGACGGTCGTAGAC+CGG 0.486773 4_3:+10438479 None:intergenic
TGTTACATCACACCATTGCT+TGG 0.490210 4_3:-10439718 Msa0629580:CDS
ATCAAAGGCTTCAACTCCTC+AGG 0.490624 4_3:+10437909 None:intergenic
GATGGTTTATATACAGAAGA+TGG 0.492031 4_3:-10440212 Msa0629580:CDS
GGAACCATGTTTGTGTTACA+AGG 0.495089 4_3:-10438593 Msa0629580:CDS
CAAGAAATGGAAGTCTTGGT+TGG 0.496663 4_3:-10438088 Msa0629580:CDS
AGCCACCAGAGAACAAAAGA+TGG 0.498645 4_3:-10440832 Msa0629580:five_prime_UTR
CTGCAAATACTGTCACAACT+TGG 0.505268 4_3:-10439750 Msa0629580:CDS
CCATGTTCTGAATTCCACAT+TGG 0.505728 4_3:-10440770 Msa0629580:five_prime_UTR
AAGTGTCATCTTCTGGGCCC+CGG 0.506775 4_3:-10438498 Msa0629580:CDS
GCAACAGTATACAGTCAAAT+GGG 0.512639 4_3:-10438614 Msa0629580:CDS
ATGTAGAATCTAACATCAAA+GGG 0.513359 4_3:-10438973 Msa0629580:CDS
GTCACCTAGATTACTTCTAC+TGG 0.514310 4_3:-10438043 Msa0629580:CDS
GACACTTAGAGTGTCGAAAA+GGG 0.515286 4_3:+10438514 None:intergenic
AAAGCCAGTAGAAGTAATCT+AGG 0.515688 4_3:+10438039 None:intergenic
GAAGATGTCAATATAAAAGC+TGG 0.515786 4_3:-10440245 Msa0629580:CDS
GCTCTCTACTTGATTGCACT+TGG 0.518546 4_3:-10439364 Msa0629580:CDS
AATACTGTCACAACTTGGTC+GGG 0.519066 4_3:-10439745 Msa0629580:CDS
TAAAAGATTTACCGAATCCA+TGG 0.520585 4_3:-10438718 Msa0629580:CDS
TTTCACTAATATCGGTCAGA+TGG 0.527060 4_3:-10438234 Msa0629580:CDS
GATGTAGAATCTAACATCAA+AGG 0.533602 4_3:-10438974 Msa0629580:CDS
AAATACTGTCACAACTTGGT+CGG 0.534850 4_3:-10439746 Msa0629580:CDS
CAGTTTGTCCTGACGTCGGA+TGG 0.535426 4_3:+10439401 None:intergenic
CTGGTGGAAGTCCTCTTACA+AGG 0.537556 4_3:-10439078 Msa0629580:CDS
CTTTATTTAATACTAATCAA+AGG 0.539658 4_3:+10437894 None:intergenic
TGGAGTGCCTGGAGTTGCAA+TGG 0.539760 4_3:-10439155 Msa0629580:CDS
CACAAACTTCTCATAGCATC+GGG 0.539806 4_3:+10438191 None:intergenic
AAAAGGGAGAGGGAGGCTGA+TGG 0.541173 4_3:+10438530 None:intergenic
CAGCTTCAAAGAATCGGAAT+CGG 0.543032 4_3:-10438413 Msa0629580:CDS
GTTACTGTGCAGATCCTCCA+TGG 0.546167 4_3:+10438701 None:intergenic
TTGCAGCATTTAGGAAACTT+GGG 0.546918 4_3:-10439039 Msa0629580:CDS
AATCTAACATCAAAGGGAGC+CGG 0.551381 4_3:-10438967 Msa0629580:CDS
GCGATAACCATTGCAACTCC+AGG 0.558801 4_3:+10439148 None:intergenic
TCACATGAAGGCTTTAGTCC+AGG 0.560189 4_3:+10439436 None:intergenic
TAGGGTTACATTACATGAAG+AGG 0.561961 4_3:-10440858 None:intergenic
ACACAAACTTCTCATAGCAT+CGG 0.567737 4_3:+10438190 None:intergenic
ACTTGCCAGAAGATCGACAT+AGG 0.569304 4_3:+10438290 None:intergenic
CTTTAGTCCAGGTGCAATTG+CGG 0.570201 4_3:+10439447 None:intergenic
TGGAGATGATGGAGATGACT+AGG 0.570250 4_3:+10438390 None:intergenic
TGTGAATCCGTGTTCATGGC+CGG 0.571537 4_3:+10438436 None:intergenic
GGATACAGATGAATGTCGGA+TGG 0.573725 4_3:-10439189 Msa0629580:CDS
AAAGCTGGTGTGAATGAAGA+TGG 0.575604 4_3:-10440230 Msa0629580:CDS
TTAGAGTGTCGAAAAGGGAG+AGG 0.583008 4_3:+10438519 None:intergenic
CCAATGTGGAATTCAGAACA+TGG 0.586283 4_3:+10440770 None:intergenic
TTGACTGTATACTGTTGCAA+AGG 0.587730 4_3:+10438619 None:intergenic
AACACACGGTTTGATACCGC+CGG 0.589392 4_3:+10439339 None:intergenic
GATACAGATGAATGTCGGAT+GGG 0.590289 4_3:-10439188 Msa0629580:CDS
CAGCCATTAGCATCACATGA+AGG 0.594111 4_3:+10439424 None:intergenic
CAGTAGAGACAGAATCCGAC+CGG 0.595827 4_3:-10438742 Msa0629580:CDS
TGGTATAGACTTCAAATACC+TGG 0.596885 4_3:-10439097 Msa0629580:CDS
TAGAGTGTCGAAAAGGGAGA+GGG 0.597883 4_3:+10438520 None:intergenic
AAATTTACCGGCCATGAACA+CGG 0.610666 4_3:-10438443 Msa0629580:CDS
AGTGTCGAAAAGGGAGAGGG+AGG 0.610825 4_3:+10438523 None:intergenic
CCAGAAGATCGACATAGGGA+TGG 0.612471 4_3:+10438295 None:intergenic
TTGATTGCACTTGGAACCGG+CGG 0.613447 4_3:-10439355 Msa0629580:CDS
CTTGTAAGAGGACTTCCACC+AGG 0.614606 4_3:+10439079 None:intergenic
AGGGTTACATTACATGAAGA+GGG 0.622745 4_3:-10440857 None:intergenic
CTGCATTTCCAAGTCCGTAA+CGG 0.628403 4_3:+10439776 None:intergenic
TTGCGAAAGGGACAATGAGA+CGG 0.631437 4_3:+10438468 None:intergenic
AATTGCTTAGATAGAGTCGT+AGG 0.631827 4_3:-10437836 Msa0629580:three_prime_UTR
CTCCATCATCTCCATGATCG+TGG 0.634231 4_3:-10438381 Msa0629580:CDS
ATTCCAGATAATATGAATAG+GGG 0.635841 4_3:-10438065 Msa0629580:CDS
GTACCGCAGTATTTCCTCGT+AGG 0.638440 4_3:-10438269 Msa0629580:CDS
GTTTGGATACAGATGAATGT+CGG 0.639388 4_3:-10439193 Msa0629580:CDS
TCATCTCCATGATCGTGGCA+GGG 0.642693 4_3:-10438376 Msa0629580:CDS
ACCGATATTAGTGAAAACTT+CGG 0.648584 4_3:+10438241 None:intergenic
TTATATTCTTCAAACCAATG+TGG 0.652743 4_3:+10440756 None:intergenic
ATTTAATACCGAGAATAAAG+CGG 0.653871 4_3:+10440129 None:intergenic
ATACAGATGAATGTCGGATG+GGG 0.656151 4_3:-10439187 Msa0629580:CDS
CTTGCCAGAAGATCGACATA+GGG 0.656281 4_3:+10438291 None:intergenic
AACACAATGGATCAACACAT+TGG 0.661215 4_3:-10438569 Msa0629580:CDS
ATGAAAACGATGAGACTGAG+AGG 0.663289 4_3:-10439291 Msa0629580:CDS
TGTGTTACAAGGCAACACAA+TGG 0.665398 4_3:-10438582 Msa0629580:CDS
TATAGACTTCAAATACCTGG+TGG 0.669427 4_3:-10439094 Msa0629580:CDS
GCTGCAGTTTGTCCTGACGT+CGG 0.674367 4_3:+10439397 None:intergenic
AATGAGACGGTCGTAGACCG+GGG 0.680218 4_3:+10438481 None:intergenic
AACCGGCAAATATTTCTGTG+TGG 0.686574 4_3:-10437798 Msa0629580:three_prime_UTR
GTTGCCTTGTAACACAAACA+TGG 0.693293 4_3:+10438589 None:intergenic
CTATCAGTGATGCCCAAACG+GGG 0.694144 4_3:+10438642 None:intergenic
AAACTTCGGCAGCACCTACG+AGG 0.701293 4_3:+10438255 None:intergenic
GTACCTAGATGAGTAACCTG+AGG 0.703319 4_3:-10437925 Msa0629580:three_prime_UTR
AAGATTTACCGAATCCATGG+AGG 0.715910 4_3:-10438715 Msa0629580:CDS
GCACCAAAAGATGAAACACA+CGG 0.721405 4_3:+10439325 None:intergenic
CTGTATCCAAACCAACACCG+AGG 0.799571 4_3:+10439204 None:intergenic
GCACCTACGAGGAAATACTG+CGG 0.832048 4_3:+10438266 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAAAAATTATTATAT+TGG + chr4_3:10438066-10438085 None:intergenic 0.0%
!! ATTGAATTCATATAAATATA+TGG + chr4_3:10438225-10438244 None:intergenic 10.0%
!! CATTATAATCAAAATAACAA+AGG + chr4_3:10437994-10438013 None:intergenic 15.0%
!!! ATTTTGATTATAATGAATTC+TGG - chr4_3:10437999-10438018 Msa0629580:CDS 15.0%
!!! TTTTGATTATAATGAATTCT+GGG - chr4_3:10438000-10438019 Msa0629580:CDS 15.0%
!!! TAATTATTTCTCATTATGTA+TGG - chr4_3:10438082-10438101 Msa0629580:CDS 15.0%
!!! TTTTACTTCTCAATTAATAT+CGG - chr4_3:10439387-10439406 Msa0629580:CDS 15.0%
!! CATAAAGATGAATATAAAAA+TGG + chr4_3:10439727-10439746 None:intergenic 15.0%
!!! CTTTATTTAATACTAATCAA+AGG + chr4_3:10440737-10440756 None:intergenic 15.0%
!! AAGTATTAGAGAAAAATGTA+GGG + chr4_3:10437920-10437939 None:intergenic 20.0%
!! AATATATCAGATTAACATCA+AGG + chr4_3:10438133-10438152 None:intergenic 20.0%
!! TCAACATTAAAAAGACAAAT+AGG + chr4_3:10438179-10438198 None:intergenic 20.0%
!!! TTGTTGAAATGTTTTGATAA+AGG - chr4_3:10439028-10439047 Msa0629580:CDS 20.0%
! TTATATTCTTCAAACCAATG+TGG + chr4_3:10437875-10437894 None:intergenic 25.0%
!!! ACATTGGTTTGAAGAATATA+AGG - chr4_3:10437874-10437893 Msa0629580:three_prime_UTR 25.0%
! CAAGTATTAGAGAAAAATGT+AGG + chr4_3:10437921-10437940 None:intergenic 25.0%
!! CTACATTTTTCTCTAATACT+TGG - chr4_3:10437919-10437938 Msa0629580:three_prime_UTR 25.0%
! TCTACATTTGTATGCATTAT+AGG - chr4_3:10437949-10437968 Msa0629580:exon 25.0%
! CTCTGATTATGTTTACTAAT+TGG - chr4_3:10438284-10438303 Msa0629580:CDS 25.0%
! ATTTAATACCGAGAATAAAG+CGG + chr4_3:10438502-10438521 None:intergenic 25.0%
! TCATATCTGATATATATTCC+CGG - chr4_3:10438554-10438573 Msa0629580:CDS 25.0%
! CAACTTGTGTTTAAATACTA+CGG + chr4_3:10438581-10438600 None:intergenic 25.0%
! GCTTGAATAATAGAAATGTA+CGG - chr4_3:10438617-10438636 Msa0629580:CDS 25.0%
! ATTCTAGTACAAGAATTGTT+AGG + chr4_3:10438740-10438759 None:intergenic 25.0%
! TAATGAATGTTGTGAAAGAT+TGG - chr4_3:10438786-10438805 Msa0629580:intron 25.0%
! TATTTATGTCATTGTAAGCT+CGG - chr4_3:10438990-10439009 Msa0629580:CDS 25.0%
! AGTTCATATTTAAATGTCTG+AGG - chr4_3:10439078-10439097 Msa0629580:CDS 25.0%
! GAAGTAAAACCAATTGAAAA+AGG + chr4_3:10439376-10439395 None:intergenic 25.0%
!! GCCATTATATTTTTCTTCAT+CGG - chr4_3:10439501-10439520 Msa0629580:intron 25.0%
! ACCGATGAAGAAAAATATAA+TGG + chr4_3:10439505-10439524 None:intergenic 25.0%
! ATGTAGAATCTAACATCAAA+GGG - chr4_3:10439655-10439674 Msa0629580:CDS 25.0%
! ACAAAAGTTACTACAAGAAA+TGG - chr4_3:10440527-10440546 Msa0629580:intron 25.0%
! GATTCCAGATAATATGAATA+GGG - chr4_3:10440562-10440581 Msa0629580:intron 25.0%
! ATTCCAGATAATATGAATAG+GGG - chr4_3:10440563-10440582 Msa0629580:intron 25.0%
GGTTTGAAGAATATAAGGTT+CGG - chr4_3:10437879-10437898 Msa0629580:three_prime_UTR 30.0%
GCAAATCAATTGTTCTACTA+TGG - chr4_3:10438361-10438380 Msa0629580:CDS 30.0%
GAAGATGTCAATATAAAAGC+TGG - chr4_3:10438383-10438402 Msa0629580:CDS 30.0%
GATGGTTTATATACAGAAGA+TGG - chr4_3:10438416-10438435 Msa0629580:CDS 30.0%
! ATTTCCTGTCTTTTTCTTGT+TGG + chr4_3:10438468-10438487 None:intergenic 30.0%
ACAAGAAAAAGACAGGAAAT+TGG - chr4_3:10438468-10438487 Msa0629580:CDS 30.0%
!!! TTTTTAGCTGATGCATACTT+GGG - chr4_3:10438937-10438956 Msa0629580:intron 30.0%
AAGAGGGAAAAACATGTTAA+AGG - chr4_3:10439112-10439131 Msa0629580:CDS 30.0%
CAAGTAGAGAGCTATAAAAA+GGG + chr4_3:10439256-10439275 None:intergenic 30.0%
TCAAGTAGAGAGCTATAAAA+AGG + chr4_3:10439257-10439276 None:intergenic 30.0%
! TCTCATCGTTTTCATCAAAT+TGG + chr4_3:10439332-10439351 None:intergenic 30.0%
!!! AGAAAAGCTCCTTTTTCAAT+TGG - chr4_3:10439364-10439383 Msa0629580:CDS 30.0%
AAGTTATTGTTGCAGCATTT+AGG - chr4_3:10439580-10439599 Msa0629580:intron 30.0%
TAAAGAAGAGACTTATCATC+TGG + chr4_3:10439624-10439643 None:intergenic 30.0%
GATGTAGAATCTAACATCAA+AGG - chr4_3:10439654-10439673 Msa0629580:CDS 30.0%
AAACAAATTTCCCTGATTTC+AGG - chr4_3:10439847-10439866 Msa0629580:intron 30.0%
!! GATTCGGTAAATCTTTTATC+CGG + chr4_3:10439908-10439927 None:intergenic 30.0%
TAAAAGATTTACCGAATCCA+TGG - chr4_3:10439910-10439929 Msa0629580:intron 30.0%
! GCTGATGGAATTTTGAATTT+AGG + chr4_3:10440086-10440105 None:intergenic 30.0%
!!! GATCATAGTAGTTGTTTTTG+CGG + chr4_3:10440307-10440326 None:intergenic 30.0%
ACCGATATTAGTGAAAACTT+CGG + chr4_3:10440390-10440409 None:intergenic 30.0%
GGATTCCAGATAATATGAAT+AGG - chr4_3:10440561-10440580 Msa0629580:intron 30.0%
! TGTATCTATTGATTGCTAAC+AGG - chr4_3:10440642-10440661 Msa0629580:intron 30.0%
ACAAAAAAGTGACTCATCTT+AGG - chr4_3:10440672-10440691 Msa0629580:intron 30.0%
GGAAATGTTTGTATCTAAAC+CGG - chr4_3:10440813-10440832 Msa0629580:five_prime_UTR 30.0%
! CTTTTCCATCTTTTGTTCTC+TGG + chr4_3:10437804-10437823 None:intergenic 35.0%
TCTTCGCAACCACAAATTAA+CGG + chr4_3:10438328-10438347 None:intergenic 35.0%
TGTGTTTAAATACTACGGTC+CGG + chr4_3:10438576-10438595 None:intergenic 35.0%
!! TGTGAAAGATTGGCTTACTA+TGG - chr4_3:10438796-10438815 Msa0629580:intron 35.0%
AAATACTGTCACAACTTGGT+CGG - chr4_3:10438882-10438901 Msa0629580:intron 35.0%
CAAGTATGCATCAGCTAAAA+AGG + chr4_3:10438938-10438957 None:intergenic 35.0%
!!! CTTTTTAGCTGATGCATACT+TGG - chr4_3:10438936-10438955 Msa0629580:intron 35.0%
TGAGGTTCACAGTAATAAGA+GGG - chr4_3:10439096-10439115 Msa0629580:CDS 35.0%
! AAACCGTGTGTTTCATCTTT+TGG - chr4_3:10439300-10439319 Msa0629580:CDS 35.0%
GTTTGGATACAGATGAATGT+CGG - chr4_3:10439435-10439454 Msa0629580:CDS 35.0%
! TTTTCTTCATCGGTAGTAGA+TGG - chr4_3:10439511-10439530 Msa0629580:intron 35.0%
TGGTATAGACTTCAAATACC+TGG - chr4_3:10439531-10439550 Msa0629580:intron 35.0%
TATAGACTTCAAATACCTGG+TGG - chr4_3:10439534-10439553 Msa0629580:intron 35.0%
ACTTGACAAATCCTTGTAAG+AGG + chr4_3:10439564-10439583 None:intergenic 35.0%
TTGCAGCATTTAGGAAACTT+GGG - chr4_3:10439589-10439608 Msa0629580:intron 35.0%
TATCCAAACACCTGAAATCA+GGG + chr4_3:10439860-10439879 None:intergenic 35.0%
TTATCCAAACACCTGAAATC+AGG + chr4_3:10439861-10439880 None:intergenic 35.0%
! TGATTTCAGGTGTTTGGATA+AGG - chr4_3:10439860-10439879 Msa0629580:intron 35.0%
TTGACTGTATACTGTTGCAA+AGG + chr4_3:10440012-10440031 None:intergenic 35.0%
TGCAACAGTATACAGTCAAA+TGG - chr4_3:10440013-10440032 Msa0629580:intron 35.0%
GCAACAGTATACAGTCAAAT+GGG - chr4_3:10440014-10440033 Msa0629580:intron 35.0%
AACACAATGGATCAACACAT+TGG - chr4_3:10440059-10440078 Msa0629580:intron 35.0%
! CGGTAAATTTTCTTGCGAAA+GGG + chr4_3:10440175-10440194 None:intergenic 35.0%
CCTTTCGCAAGAAAATTTAC+CGG - chr4_3:10440173-10440192 Msa0629580:CDS 35.0%
TTCACACAGCTTCAAAGAAT+CGG - chr4_3:10440209-10440228 Msa0629580:CDS 35.0%
! GCCGAAGTTTTCACTAATAT+CGG - chr4_3:10440386-10440405 Msa0629580:intron 35.0%
TTTCACTAATATCGGTCAGA+TGG - chr4_3:10440394-10440413 Msa0629580:intron 35.0%
ACACAAACTTCTCATAGCAT+CGG + chr4_3:10440441-10440460 None:intergenic 35.0%
TTCACTCACAACTAATGCAT+TGG - chr4_3:10440469-10440488 Msa0629580:intron 35.0%
TCACTCACAACTAATGCATT+GGG - chr4_3:10440470-10440489 Msa0629580:intron 35.0%
ACTACAAGAAATGGAAGTCT+TGG - chr4_3:10440536-10440555 Msa0629580:intron 35.0%
TGACCCCTATTCATATTATC+TGG + chr4_3:10440569-10440588 None:intergenic 35.0%
AAAGCCAGTAGAAGTAATCT+AGG + chr4_3:10440592-10440611 None:intergenic 35.0%
AATTGCTTAGATAGAGTCGT+AGG - chr4_3:10440792-10440811 Msa0629580:five_prime_UTR 35.0%
ATCCACACAGAAATATTTGC+CGG + chr4_3:10440835-10440854 None:intergenic 35.0%
! TTCCATCTTTTGTTCTCTGG+TGG + chr4_3:10437801-10437820 None:intergenic 40.0%
CCAATGTGGAATTCAGAACA+TGG + chr4_3:10437861-10437880 None:intergenic 40.0%
! CCATGTTCTGAATTCCACAT+TGG - chr4_3:10437858-10437877 Msa0629580:three_prime_UTR 40.0%
! ACAAACACGCCGTTAATTTG+TGG - chr4_3:10438316-10438335 Msa0629580:CDS 40.0%
AAAGCTGGTGTGAATGAAGA+TGG - chr4_3:10438398-10438417 Msa0629580:CDS 40.0%
! CTGTCTTTTTCTTGTTGGCA+GGG + chr4_3:10438463-10438482 None:intergenic 40.0%
GTGTTTAAATACTACGGTCC+GGG + chr4_3:10438575-10438594 None:intergenic 40.0%
CTATGGCATGAGTACAAACT+TGG - chr4_3:10438813-10438832 Msa0629580:intron 40.0%
AACTATCTCGAAGACCGTTA+CGG - chr4_3:10438838-10438857 Msa0629580:intron 40.0%
CTGCAAATACTGTCACAACT+TGG - chr4_3:10438878-10438897 Msa0629580:intron 40.0%
AATACTGTCACAACTTGGTC+GGG - chr4_3:10438883-10438902 Msa0629580:intron 40.0%
TGTTACATCACACCATTGCT+TGG - chr4_3:10438910-10438929 Msa0629580:intron 40.0%
ATGCATACTTGGGAAGATAC+TGG - chr4_3:10438947-10438966 Msa0629580:CDS 40.0%
CTGAGGTTCACAGTAATAAG+AGG - chr4_3:10439095-10439114 Msa0629580:CDS 40.0%
TCGTTGTTTGTGTACTTTGC+AGG - chr4_3:10439137-10439156 Msa0629580:CDS 40.0%
CGTTGTTTGTGTACTTTGCA+GGG - chr4_3:10439138-10439157 Msa0629580:CDS 40.0%
TTTGTGTACTTTGCAGGGAA+TGG - chr4_3:10439143-10439162 Msa0629580:CDS 40.0%
AAGCCTTCATGTGATGCTAA+TGG - chr4_3:10439201-10439220 Msa0629580:CDS 40.0%
TACTTGATTGCACTTGGAAC+CGG - chr4_3:10439270-10439289 Msa0629580:CDS 40.0%
GCACCAAAAGATGAAACACA+CGG + chr4_3:10439306-10439325 None:intergenic 40.0%
ATGAAAACGATGAGACTGAG+AGG - chr4_3:10439337-10439356 Msa0629580:CDS 40.0%
GATACAGATGAATGTCGGAT+GGG - chr4_3:10439440-10439459 Msa0629580:CDS 40.0%
ATACAGATGAATGTCGGATG+GGG - chr4_3:10439441-10439460 Msa0629580:CDS 40.0%
GTTGCAGCATTTAGGAAACT+TGG - chr4_3:10439588-10439607 Msa0629580:intron 40.0%
AATCTAACATCAAAGGGAGC+CGG - chr4_3:10439661-10439680 Msa0629580:CDS 40.0%
! CATCTGTGTGTTCAAGTTTC+CGG + chr4_3:10439683-10439702 None:intergenic 40.0%
! TTTCCCTGATTTCAGGTGTT+TGG - chr4_3:10439854-10439873 Msa0629580:intron 40.0%
! CGGTAAATCTTTTATCCGGT+CGG + chr4_3:10439904-10439923 None:intergenic 40.0%
AAGATTTACCGAATCCATGG+AGG - chr4_3:10439913-10439932 Msa0629580:intron 40.0%
GGAACCATGTTTGTGTTACA+AGG - chr4_3:10440035-10440054 Msa0629580:intron 40.0%
GTTGCCTTGTAACACAAACA+TGG + chr4_3:10440042-10440061 None:intergenic 40.0%
TGTGTTACAAGGCAACACAA+TGG - chr4_3:10440046-10440065 Msa0629580:intron 40.0%
!! GACACTTAGAGTGTCGAAAA+GGG + chr4_3:10440117-10440136 None:intergenic 40.0%
!! TGACACTTAGAGTGTCGAAA+AGG + chr4_3:10440118-10440137 None:intergenic 40.0%
ACACTCTAAGTGTCATCTTC+TGG - chr4_3:10440123-10440142 Msa0629580:intron 40.0%
CACTCTAAGTGTCATCTTCT+GGG - chr4_3:10440124-10440143 Msa0629580:intron 40.0%
! CCGGTAAATTTTCTTGCGAA+AGG + chr4_3:10440176-10440195 None:intergenic 40.0%
AAATTTACCGGCCATGAACA+CGG - chr4_3:10440185-10440204 Msa0629580:CDS 40.0%
CAGCTTCAAAGAATCGGAAT+CGG - chr4_3:10440215-10440234 Msa0629580:CDS 40.0%
!! TGTTTTTGCGGACAATGTCA+AGG + chr4_3:10440295-10440314 None:intergenic 40.0%
! GGTCAGATGGAGTTCTTTTA+TGG - chr4_3:10440407-10440426 Msa0629580:intron 40.0%
! GATGGAGTTCTTTTATGGTC+AGG - chr4_3:10440412-10440431 Msa0629580:intron 40.0%
CACAAACTTCTCATAGCATC+GGG + chr4_3:10440440-10440459 None:intergenic 40.0%
CAAGAAATGGAAGTCTTGGT+TGG - chr4_3:10440540-10440559 Msa0629580:intron 40.0%
GTCACCTAGATTACTTCTAC+TGG - chr4_3:10440585-10440604 Msa0629580:intron 40.0%
CGACTCTATCTAAGCAATTC+AGG + chr4_3:10440791-10440810 None:intergenic 40.0%
! AACCGGCAAATATTTCTGTG+TGG - chr4_3:10440830-10440849 Msa0629580:five_prime_UTR 40.0%
AGCCACCAGAGAACAAAAGA+TGG - chr4_3:10437796-10437815 Msa0629580:three_prime_UTR 45.0%
! CCTGTCTTTTTCTTGTTGGC+AGG + chr4_3:10438464-10438483 None:intergenic 45.0%
CCTGCCAACAAGAAAAAGAC+AGG - chr4_3:10438461-10438480 Msa0629580:CDS 45.0%
!! AAAGCGTGCCGCTTTATTCT+CGG - chr4_3:10438491-10438510 Msa0629580:CDS 45.0%
ATCACGCGACAAATTGTTGC+AGG - chr4_3:10438763-10438782 Msa0629580:intron 45.0%
CTGCATTTCCAAGTCCGTAA+CGG + chr4_3:10438855-10438874 None:intergenic 45.0%
GCTAAAAAGGCTCCAAGCAA+TGG + chr4_3:10438925-10438944 None:intergenic 45.0%
! ACATTTTCCGCAATTGCACC+TGG - chr4_3:10439174-10439193 Msa0629580:CDS 45.0%
CTTTAGTCCAGGTGCAATTG+CGG + chr4_3:10439184-10439203 None:intergenic 45.0%
! TCACATGAAGGCTTTAGTCC+AGG + chr4_3:10439195-10439214 None:intergenic 45.0%
CAGCCATTAGCATCACATGA+AGG + chr4_3:10439207-10439226 None:intergenic 45.0%
GCTCTCTACTTGATTGCACT+TGG - chr4_3:10439264-10439283 Msa0629580:CDS 45.0%
GGATACAGATGAATGTCGGA+TGG - chr4_3:10439439-10439458 Msa0629580:CDS 45.0%
!! TAGAGTGTCGAAAAGGGAGA+GGG + chr4_3:10440111-10440130 None:intergenic 45.0%
!! TTAGAGTGTCGAAAAGGGAG+AGG + chr4_3:10440112-10440131 None:intergenic 45.0%
TTGCGAAAGGGACAATGAGA+CGG + chr4_3:10440163-10440182 None:intergenic 45.0%
!! TGGAGATGATGGAGATGACT+AGG + chr4_3:10440241-10440260 None:intergenic 45.0%
! TTTTGCGGACAATGTCAAGG+CGG + chr4_3:10440292-10440311 None:intergenic 45.0%
CTTGCCAGAAGATCGACATA+GGG + chr4_3:10440340-10440359 None:intergenic 45.0%
ACTTGCCAGAAGATCGACAT+AGG + chr4_3:10440341-10440360 None:intergenic 45.0%
GTACCTAGATGAGTAACCTG+AGG - chr4_3:10440703-10440722 Msa0629580:intron 45.0%
ACTCCTCAGGTTACTCATCT+AGG + chr4_3:10440709-10440728 None:intergenic 45.0%
ATCAAAGGCTTCAACTCCTC+AGG + chr4_3:10440722-10440741 None:intergenic 45.0%
CTACGTCTACGAGTACAGAG+TGG + chr4_3:10438646-10438665 None:intergenic 50.0%
CACTCTGTACTCGTAGACGT+AGG - chr4_3:10438644-10438663 Msa0629580:CDS 50.0%
CTCGTAGACGTAGGCACATT+TGG - chr4_3:10438653-10438672 Msa0629580:CDS 50.0%
TGTTAGGACGACAACAACGC+TGG + chr4_3:10438724-10438743 None:intergenic 50.0%
TTGATTGCACTTGGAACCGG+CGG - chr4_3:10439273-10439292 Msa0629580:CDS 50.0%
AACACACGGTTTGATACCGC+CGG + chr4_3:10439292-10439311 None:intergenic 50.0%
! ACTCATTGCTTCCTCGGTGT+TGG - chr4_3:10439413-10439432 Msa0629580:CDS 50.0%
!! TTGCTTCCTCGGTGTTGGTT+TGG - chr4_3:10439418-10439437 Msa0629580:CDS 50.0%
CTGTATCCAAACCAACACCG+AGG + chr4_3:10439427-10439446 None:intergenic 50.0%
AGATGAATGTCGGATGGGGA+TGG - chr4_3:10439445-10439464 Msa0629580:CDS 50.0%
GATGAATGTCGGATGGGGAT+GGG - chr4_3:10439446-10439465 Msa0629580:CDS 50.0%
ATGAATGTCGGATGGGGATG+GGG - chr4_3:10439447-10439466 Msa0629580:CDS 50.0%
GCGATAACCATTGCAACTCC+AGG + chr4_3:10439483-10439502 None:intergenic 50.0%
CTTGTAAGAGGACTTCCACC+AGG + chr4_3:10439552-10439571 None:intergenic 50.0%
CTGGTGGAAGTCCTCTTACA+AGG - chr4_3:10439550-10439569 Msa0629580:intron 50.0%
CAGTAGAGACAGAATCCGAC+CGG - chr4_3:10439886-10439905 Msa0629580:intron 50.0%
GTGCAGATCCTCCATGGATT+CGG + chr4_3:10439924-10439943 None:intergenic 50.0%
GTTACTGTGCAGATCCTCCA+TGG + chr4_3:10439930-10439949 None:intergenic 50.0%
TCGTTCGTTTACTCCCCGTT+TGG - chr4_3:10439973-10439992 Msa0629580:intron 50.0%
CGTTCGTTTACTCCCCGTTT+GGG - chr4_3:10439974-10439993 Msa0629580:intron 50.0%
CTATCAGTGATGCCCAAACG+GGG + chr4_3:10439989-10440008 None:intergenic 50.0%
GCTATCAGTGATGCCCAAAC+GGG + chr4_3:10439990-10440009 None:intergenic 50.0%
GGCTATCAGTGATGCCCAAA+CGG + chr4_3:10439991-10440010 None:intergenic 50.0%
ACAATGAGACGGTCGTAGAC+CGG + chr4_3:10440152-10440171 None:intergenic 50.0%
TGTGAATCCGTGTTCATGGC+CGG + chr4_3:10440195-10440214 None:intergenic 50.0%
GCTGTGTGAATCCGTGTTCA+TGG + chr4_3:10440199-10440218 None:intergenic 50.0%
CTCCATCATCTCCATGATCG+TGG - chr4_3:10440247-10440266 Msa0629580:CDS 50.0%
TGCCACGATCATGGAGATGA+TGG + chr4_3:10440252-10440271 None:intergenic 50.0%
ATCATCTCCATGATCGTGGC+AGG - chr4_3:10440251-10440270 Msa0629580:CDS 50.0%
TCATCTCCATGATCGTGGCA+GGG - chr4_3:10440252-10440271 Msa0629580:exon 50.0%
TAGAATCCCTGCCACGATCA+TGG + chr4_3:10440261-10440280 None:intergenic 50.0%
CCAGAAGATCGACATAGGGA+TGG + chr4_3:10440336-10440355 None:intergenic 50.0%
CCATCCCTATGTCGATCTTC+TGG - chr4_3:10440333-10440352 Msa0629580:intron 50.0%
GTACCGCAGTATTTCCTCGT+AGG - chr4_3:10440359-10440378 Msa0629580:intron 50.0%
GCACCTACGAGGAAATACTG+CGG + chr4_3:10440365-10440384 None:intergenic 50.0%
CTCGAAGACCGTTACGGACT+TGG - chr4_3:10438844-10438863 Msa0629580:intron 55.0%
CAGTTTGTCCTGACGTCGGA+TGG + chr4_3:10439230-10439249 None:intergenic 55.0%
GCTGCAGTTTGTCCTGACGT+CGG + chr4_3:10439234-10439253 None:intergenic 55.0%
CGGTGCACTCATTGCTTCCT+CGG - chr4_3:10439407-10439426 Msa0629580:CDS 55.0%
! TGGAGTGCCTGGAGTTGCAA+TGG - chr4_3:10439473-10439492 Msa0629580:intron 55.0%
AAAAGGGAGAGGGAGGCTGA+TGG + chr4_3:10440101-10440120 None:intergenic 55.0%
! AGTGTCGAAAAGGGAGAGGG+AGG + chr4_3:10440108-10440127 None:intergenic 55.0%
AAGTGTCATCTTCTGGGCCC+CGG - chr4_3:10440130-10440149 Msa0629580:intron 55.0%
AATGAGACGGTCGTAGACCG+GGG + chr4_3:10440150-10440169 None:intergenic 55.0%
CAATGAGACGGTCGTAGACC+GGG + chr4_3:10440151-10440170 None:intergenic 55.0%
AAACTTCGGCAGCACCTACG+AGG + chr4_3:10440376-10440395 None:intergenic 55.0%
AATGGCTGCCATCCGACGTC+AGG - chr4_3:10439219-10439238 Msa0629580:CDS 60.0%
! GTCGGATGGGGATGGGGTTT+TGG - chr4_3:10439453-10439472 Msa0629580:CDS 60.0%
!!! GGATGGGGTTTTGGAGTGCC+TGG - chr4_3:10439462-10439481 Msa0629580:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 10437779 10440871 10437779 ID=Msa0629580;Name=Msa0629580
chr4_3 mRNA 10437779 10440871 10437779 ID=Msa0629580-mRNA-1;Parent=Msa0629580;Name=Msa0629580-mRNA-1;_AED=0.08;_eAED=0.08;_QI=187|1|1|1|0.75|0.6|5|174|576
chr4_3 exon 10437779 10438781 10437779 ID=Msa0629580-mRNA-1:exon:3230;Parent=Msa0629580-mRNA-1
chr4_3 exon 10438941 10439491 10438941 ID=Msa0629580-mRNA-1:exon:3229;Parent=Msa0629580-mRNA-1
chr4_3 exon 10439648 10439865 10439648 ID=Msa0629580-mRNA-1:exon:3228;Parent=Msa0629580-mRNA-1
chr4_3 exon 10440138 10440340 10440138 ID=Msa0629580-mRNA-1:exon:3227;Parent=Msa0629580-mRNA-1
chr4_3 exon 10440755 10440871 10440755 ID=Msa0629580-mRNA-1:exon:3226;Parent=Msa0629580-mRNA-1
chr4_3 five_prime_UTR 10440755 10440871 10440755 ID=Msa0629580-mRNA-1:five_prime_utr;Parent=Msa0629580-mRNA-1
chr4_3 five_prime_UTR 10440271 10440340 10440271 ID=Msa0629580-mRNA-1:five_prime_utr;Parent=Msa0629580-mRNA-1
chr4_3 CDS 10440138 10440270 10440138 ID=Msa0629580-mRNA-1:cds;Parent=Msa0629580-mRNA-1
chr4_3 CDS 10439648 10439865 10439648 ID=Msa0629580-mRNA-1:cds;Parent=Msa0629580-mRNA-1
chr4_3 CDS 10438941 10439491 10438941 ID=Msa0629580-mRNA-1:cds;Parent=Msa0629580-mRNA-1
chr4_3 CDS 10437953 10438781 10437953 ID=Msa0629580-mRNA-1:cds;Parent=Msa0629580-mRNA-1
chr4_3 three_prime_UTR 10437779 10437952 10437779 ID=Msa0629580-mRNA-1:three_prime_utr;Parent=Msa0629580-mRNA-1
Gene Sequence

>Msa0629580

ATGGAAGATGTCAATATAAAAGCTGGTGTGAATGAAGATGGTTTATATACAGAAGATGGAACAATTGACATTCACAAAAACCCTGCCAACAAGAAAAAGACAGGAAATTGGAAAGCGTGCCGCTTTATTCTCGGTAATGAATGTTGTGAAAGATTGGCTTACTATGGCATGAGTACAAACTTGGTGAACTATCTCGAAGACCGTTACGGACTTGGAAATGCAGCTGCTGCAAATACTGTCACAACTTGGTCGGGAACATGTTACATCACACCATTGCTTGGAGCCTTTTTAGCTGATGCATACTTGGGAAGATACTGGACAATTGCTAGTTTCTCAAGTATTTATGTCATTGGAATGGCACTTTTAACATTTTCCGCAATTGCACCTGGACTAAAGCCTTCATGTGATGCTAATGGCTGCCATCCGACGTCAGGACAAACTGCAGCCCTTTTTATAGCTCTCTACTTGATTGCACTTGGAACCGGCGGTATCAAACCGTGTGTTTCATCTTTTGGTGCTGACCAATTTGATGAAAACGATGAGACTGAGAGGAAAAAGAAAAGCTCCTTTTTCAATTGGTTTTACTTCTCAATTAATATCGGTGCACTCATTGCTTCCTCGGTGTTGGTTTGGATACAGATGAATGTCGGATGGGGATGGGGTTTTGGAGTGCCTGGAGTTGCAATGGTTATCGCCATTATATTTTTCTTCATCGGTAGTAGATGGTATAGACTTCAAATACCTGGTGGAAGTCCTCTTACAAGGATTTGTCAAGTTATTGTTGCAGCATTTAGGAAACTTGGGCAACAAGTTCCAGATGATAAGTCTCTTCTTTATGAGACTACTGATGTAGAATCTAACATCAAAGGGAGCCGGAAACTTGAACACACAGATGAATTGAAGTGTTTGGATAAGGCAGCAGTAGAGACAGAATCCGACCGGATAAAAGATTTACCGAATCCATGGAGGATCTGCACAGTAACACAAGTCGAAGAACTCAAATCAATCGTTCGTTTACTCCCCGTTTGGGCATCACTGATAGCCTTTGCAACAGTATACAGTCAAATGGGAACCATGTTTGTGTTACAAGGCAACACAATGGATCAACACATTGGTCCTAAATTCAAAATTCCATCAGCCTCCCTCTCCCTTTTCGACACTCTAAGTGTCATCTTCTGGGCCCCGGTCTACGACCGTCTCATTGTCCCTTTCGCAAGAAAATTTACCGGCCATGAACACGGATTCACACAGCTTCAAAGAATCGGAATCGGCCTAGTCATCTCCATCATCTCCATGATCGTGGCAGGGATTCTAGAAGTCATCCGCCTTGACATTGTCCGCAAAAACAACTACTATGATCTCGAAACCATCCCTATGTCGATCTTCTGGCAAGTACCGCAGTATTTCCTCGTAGGTGCTGCCGAAGTTTTCACTAATATCGGTCAGATGGAGTTCTTTTATGGTCAGGCACCCGATGCTATGAGAAGTTTGTGTGCTGCACTTTCACTCACAACTAATGCATTGGGAAATTATGTGAGTACATTGCTTGTTACTATTGTGACAAAAGTTACTACAAGAAATGGAAGTCTTGGTTGGATTCCAGATAATATGAATAGGGGTCACCTAGATTACTTCTACTGGCTTTTGACAATTCTTAGTTTGATCAACTTCATTGTGTATCTATTGATTGCTAACAGGTATACATACAAAAAAGTGACTCATCTTAGGTGA
Protein sequence

>Msa0629580

MEDVNIKAGVNEDGLYTEDGTIDIHKNPANKKKTGNWKACRFILGNECCERLAYYGMSTNLVNYLEDRYGLGNAAAANTVTTWSGTCYITPLLGAFLADAYLGRYWTIASFSSIYVIGMALLTFSAIAPGLKPSCDANGCHPTSGQTAALFIALYLIALGTGGIKPCVSSFGADQFDENDETERKKKSSFFNWFYFSINIGALIASSVLVWIQMNVGWGWGFGVPGVAMVIAIIFFFIGSRWYRLQIPGGSPLTRICQVIVAAFRKLGQQVPDDKSLLYETTDVESNIKGSRKLEHTDELKCLDKAAVETESDRIKDLPNPWRICTVTQVEELKSIVRLLPVWASLIAFATVYSQMGTMFVLQGNTMDQHIGPKFKIPSASLSLFDTLSVIFWAPVYDRLIVPFARKFTGHEHGFTQLQRIGIGLVISIISMIVAGILEVIRLDIVRKNNYYDLETIPMSIFWQVPQYFLVGAAEVFTNIGQMEFFYGQAPDAMRSLCAALSLTTNALGNYVSTLLVTIVTKVTTRNGSLGWIPDNMNRGHLDYFYWLLTILSLINFIVYLLIANRYTYKKVTHLR*