AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0295570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0295570 MtrunA17_Chr2g0317741 89.041 73 8 0 1 73 1 73 1.49e-37 119
Msa0295570 MtrunA17_Chr2g0317751 66.216 74 22 2 1 73 1 72 5.20e-30 100
Msa0295570 MtrunA17_Chr8g0385311 37.333 75 44 2 1 73 1 74 2.87e-12 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATATTCATCACTAAGTA+TGG 0.332590 2_3:+48177535 Msa0295570:exon
ATATGATAAAAGTGCATAAC+TGG 0.354165 2_3:-48179670 None:intergenic
TGCAGAAACATTGAAATCTA+TGG 0.367492 2_3:-48179432 None:intergenic
AGCAAGGACTTGTGAGCATT+TGG 0.402994 2_3:+48179324 Msa0295570:CDS
GAACGAGGAAGAGGAAGCAC+AGG 0.435069 2_3:-48177577 None:intergenic
CTTGCAAGTTGCATGCATCT+TGG 0.455133 2_3:-48179500 None:intergenic
AACAAAGCACACTTAATAAG+TGG 0.478955 2_3:+48179403 Msa0295570:CDS
CGACAAAGAGAACGAGGAAG+AGG 0.484363 2_3:-48177586 None:intergenic
TTAAGTGTTTGTGTCTAATG+TGG 0.522872 2_3:-48177484 None:intergenic
CATTTGGCTGATACATATAG+AGG 0.536909 2_3:+48179340 Msa0295570:CDS
AGTTGCATGCATCTTGGTGT+TGG 0.540849 2_3:-48179494 None:intergenic
ATGGAGAAGAGATCAGTAGC+TGG 0.575142 2_3:+48177554 Msa0295570:CDS
ACGATATTGATGAGTACCTA+TGG 0.577282 2_3:-48179637 None:intergenic
CATCTATGAATTATGTCCAT+AGG 0.585201 2_3:+48179621 Msa0295570:three_prime_UTR
GCACTCAAAACTGTTAAGTG+TGG 0.610534 2_3:+48179452 Msa0295570:exon
CAGCTAGCATCTGTGAAGCA+TGG 0.627719 2_3:-48179364 None:intergenic
CATGGATGCAGAAGAAATTG+TGG 0.636627 2_3:+48179294 Msa0295570:intron
AAATTGTGGTGATTGAAGCA+AGG 0.641556 2_3:+48179308 Msa0295570:CDS
AAACTGTTAAGTGTGGAATG+TGG 0.677412 2_3:+48179459 Msa0295570:exon
TACGTGCGACAAAGAGAACG+AGG 0.732491 2_3:-48177592 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATAAAAAAAAAAGAAG+AGG - chr2_3:48177687-48177706 None:intergenic 10.0%
!!! TTAAAGTATATCTTTAATTT+CGG + chr2_3:48177726-48177745 Msa0295570:intron 10.0%
!! AATAACAATAATAATTAGAT+GGG - chr2_3:48177878-48177897 None:intergenic 10.0%
!! TAATAACAATAATAATTAGA+TGG - chr2_3:48177879-48177898 None:intergenic 10.0%
!! TTTATTCTAATATTGTTTAA+TGG + chr2_3:48179269-48179288 Msa0295570:intron 10.0%
!! ATTTATAAAAAAAAAAGAAG+AGG - chr2_3:48177687-48177706 None:intergenic 10.0%
!!! TTAAAGTATATCTTTAATTT+CGG + chr2_3:48177726-48177745 Msa0295570:intron 10.0%
!! AATAACAATAATAATTAGAT+GGG - chr2_3:48177878-48177897 None:intergenic 10.0%
!! TAATAACAATAATAATTAGA+TGG - chr2_3:48177879-48177898 None:intergenic 10.0%
!! TTTATTCTAATATTGTTTAA+TGG + chr2_3:48179269-48179288 Msa0295570:intron 10.0%
!!! TCTTTCTCTAATTTAATTTT+TGG + chr2_3:48177825-48177844 Msa0295570:intron 15.0%
!!! AATTAAGTTTTTTTTATAGC+AGG + chr2_3:48177949-48177968 Msa0295570:intron 15.0%
!!! ATTTTTTATTTGTAAATGTC+AGG + chr2_3:48178144-48178163 Msa0295570:intron 15.0%
!! TATATAAATATTACTTTGTC+AGG - chr2_3:48178171-48178190 None:intergenic 15.0%
!! TTAAATATATTTGTGTTTGT+TGG - chr2_3:48178467-48178486 None:intergenic 15.0%
!! CTTAATTAACTATATACTAA+AGG - chr2_3:48178676-48178695 None:intergenic 15.0%
!!! TAATTAAGTTTTTTTATAGC+AGG + chr2_3:48178688-48178707 Msa0295570:intron 15.0%
!! TATATAAATATTACTTTGTC+AGG - chr2_3:48178171-48178190 None:intergenic 15.0%
!!! TCTTTCTCTAATTTAATTTT+TGG + chr2_3:48177825-48177844 Msa0295570:intron 15.0%
!!! AATTAAGTTTTTTTTATAGC+AGG + chr2_3:48177949-48177968 Msa0295570:intron 15.0%
!!! ATTTTTTATTTGTAAATGTC+AGG + chr2_3:48178144-48178163 Msa0295570:intron 15.0%
!! TATATAAATATTACTTTGTC+AGG - chr2_3:48178171-48178190 None:intergenic 15.0%
!! TTAAATATATTTGTGTTTGT+TGG - chr2_3:48178467-48178486 None:intergenic 15.0%
!! CTTAATTAACTATATACTAA+AGG - chr2_3:48178676-48178695 None:intergenic 15.0%
!!! TAATTAAGTTTTTTTATAGC+AGG + chr2_3:48178688-48178707 Msa0295570:intron 15.0%
!! TATATAAATATTACTTTGTC+AGG - chr2_3:48178171-48178190 None:intergenic 15.0%
!! ATTATATTCATCACTAAGTA+TGG + chr2_3:48177535-48177554 Msa0295570:exon 20.0%
!! CTTAATTGACAATATACTAA+AGG - chr2_3:48177936-48177955 None:intergenic 20.0%
!! AAATAAAAAATCGACACATT+TGG - chr2_3:48178135-48178154 None:intergenic 20.0%
!!! ACTAAGCAATTAAGTTTTAA+TGG - chr2_3:48178389-48178408 None:intergenic 20.0%
!! CAAATATATTTAACCTAAGA+TGG + chr2_3:48178474-48178493 Msa0295570:intron 20.0%
!! AGCAACAATAATAATTAGAT+GGG - chr2_3:48178612-48178631 None:intergenic 20.0%
!! ATTAACTATATACTAAAGGA+AGG - chr2_3:48178672-48178691 None:intergenic 20.0%
!! AAATAAAAAATCGACACATT+TGG - chr2_3:48178135-48178154 None:intergenic 20.0%
!!! TGTCGATTTTTTATTTGTAA+AGG + chr2_3:48178139-48178158 Msa0295570:intron 20.0%
!!! ATTTTTTATTTGTAAAGGTC+AGG + chr2_3:48178894-48178913 Msa0295570:intron 20.0%
!!! ACTAAGCAATTAAGTTTTAA+TGG - chr2_3:48178389-48178408 None:intergenic 20.0%
!! TAATATTGTTTAATGGTACA+TGG + chr2_3:48179276-48179295 Msa0295570:intron 20.0%
!! ATTATATTCATCACTAAGTA+TGG + chr2_3:48177535-48177554 Msa0295570:exon 20.0%
!! CTTAATTGACAATATACTAA+AGG - chr2_3:48177936-48177955 None:intergenic 20.0%
!! AAATAAAAAATCGACACATT+TGG - chr2_3:48178135-48178154 None:intergenic 20.0%
!!! ACTAAGCAATTAAGTTTTAA+TGG - chr2_3:48178389-48178408 None:intergenic 20.0%
!! CAAATATATTTAACCTAAGA+TGG + chr2_3:48178474-48178493 Msa0295570:intron 20.0%
!! AGCAACAATAATAATTAGAT+GGG - chr2_3:48178612-48178631 None:intergenic 20.0%
!! ATTAACTATATACTAAAGGA+AGG - chr2_3:48178672-48178691 None:intergenic 20.0%
!! AAATAAAAAATCGACACATT+TGG - chr2_3:48178135-48178154 None:intergenic 20.0%
!!! TGTCGATTTTTTATTTGTAA+AGG + chr2_3:48178139-48178158 Msa0295570:intron 20.0%
!!! ATTTTTTATTTGTAAAGGTC+AGG + chr2_3:48178894-48178913 Msa0295570:intron 20.0%
!!! ACTAAGCAATTAAGTTTTAA+TGG - chr2_3:48178389-48178408 None:intergenic 20.0%
!! TAATATTGTTTAATGGTACA+TGG + chr2_3:48179276-48179295 Msa0295570:intron 20.0%
! ATTGACAATATACTAAAGGA+AGG - chr2_3:48177932-48177951 None:intergenic 25.0%
!!! TAAGTTTTTTTTATAGCAGG+AGG + chr2_3:48177952-48177971 Msa0295570:intron 25.0%
! GAAAAATTCGATTTACTAAG+TGG - chr2_3:48178333-48178352 None:intergenic 25.0%
!! GTGATATTTTTCTCTAATAG+TGG + chr2_3:48178496-48178515 Msa0295570:intron 25.0%
! ATTGTATAATAGATAGCATG+TGG - chr2_3:48178564-48178583 None:intergenic 25.0%
! CAGCAACAATAATAATTAGA+TGG - chr2_3:48178613-48178632 None:intergenic 25.0%
!!! TTAAGTTTTTTTATAGCAGG+AGG + chr2_3:48178691-48178710 Msa0295570:intron 25.0%
! GAAAAATTCGATTTACTAAG+TGG - chr2_3:48178333-48178352 None:intergenic 25.0%
!! ATATGATAAAAGTGCATAAC+TGG - chr2_3:48179673-48179692 None:intergenic 25.0%
! TAATAATTTCGATATCAACG+TGG + chr2_3:48179712-48179731 Msa0295570:three_prime_UTR 25.0%
! ATTGACAATATACTAAAGGA+AGG - chr2_3:48177932-48177951 None:intergenic 25.0%
!!! TAAGTTTTTTTTATAGCAGG+AGG + chr2_3:48177952-48177971 Msa0295570:intron 25.0%
! GAAAAATTCGATTTACTAAG+TGG - chr2_3:48178333-48178352 None:intergenic 25.0%
!! GTGATATTTTTCTCTAATAG+TGG + chr2_3:48178496-48178515 Msa0295570:intron 25.0%
! ATTGTATAATAGATAGCATG+TGG - chr2_3:48178564-48178583 None:intergenic 25.0%
! CAGCAACAATAATAATTAGA+TGG - chr2_3:48178613-48178632 None:intergenic 25.0%
!!! TTAAGTTTTTTTATAGCAGG+AGG + chr2_3:48178691-48178710 Msa0295570:intron 25.0%
! GAAAAATTCGATTTACTAAG+TGG - chr2_3:48178333-48178352 None:intergenic 25.0%
!! ATATGATAAAAGTGCATAAC+TGG - chr2_3:48179673-48179692 None:intergenic 25.0%
TTAAGTGTTTGTGTCTAATG+TGG - chr2_3:48177487-48177506 None:intergenic 30.0%
AGACATCATGTCTAAAAGAA+AGG + chr2_3:48177763-48177782 Msa0295570:intron 30.0%
AAAAGACAAATTGAACCGTT+TGG + chr2_3:48178028-48178047 Msa0295570:intron 30.0%
! CACATCTATTTCTTAGTGTA+TGG + chr2_3:48178253-48178272 Msa0295570:intron 30.0%
GAGAAAAATATCACCATCTT+AGG - chr2_3:48178490-48178509 None:intergenic 30.0%
AATAGATAGCATGTGGTAAA+TGG - chr2_3:48178557-48178576 None:intergenic 30.0%
AAAAGACAAATTGAACCGTT+TGG + chr2_3:48178028-48178047 Msa0295570:intron 30.0%
! CACATCTATTTCTTAGTGTA+TGG + chr2_3:48178253-48178272 Msa0295570:intron 30.0%
AACAAAGCACACTTAATAAG+TGG + chr2_3:48179403-48179422 Msa0295570:CDS 30.0%
TGCAGAAACATTGAAATCTA+TGG - chr2_3:48179435-48179454 None:intergenic 30.0%
CATCTATGAATTATGTCCAT+AGG + chr2_3:48179621-48179640 Msa0295570:three_prime_UTR 30.0%
TTAAGTGTTTGTGTCTAATG+TGG - chr2_3:48177487-48177506 None:intergenic 30.0%
AGACATCATGTCTAAAAGAA+AGG + chr2_3:48177763-48177782 Msa0295570:intron 30.0%
AAAAGACAAATTGAACCGTT+TGG + chr2_3:48178028-48178047 Msa0295570:intron 30.0%
! CACATCTATTTCTTAGTGTA+TGG + chr2_3:48178253-48178272 Msa0295570:intron 30.0%
GAGAAAAATATCACCATCTT+AGG - chr2_3:48178490-48178509 None:intergenic 30.0%
AATAGATAGCATGTGGTAAA+TGG - chr2_3:48178557-48178576 None:intergenic 30.0%
AAAAGACAAATTGAACCGTT+TGG + chr2_3:48178028-48178047 Msa0295570:intron 30.0%
! CACATCTATTTCTTAGTGTA+TGG + chr2_3:48178253-48178272 Msa0295570:intron 30.0%
AACAAAGCACACTTAATAAG+TGG + chr2_3:48179403-48179422 Msa0295570:CDS 30.0%
TGCAGAAACATTGAAATCTA+TGG - chr2_3:48179435-48179454 None:intergenic 30.0%
CATCTATGAATTATGTCCAT+AGG + chr2_3:48179621-48179640 Msa0295570:three_prime_UTR 30.0%
CATCAATACGTAGAAATGTG+TGG + chr2_3:48178102-48178121 Msa0295570:intron 35.0%
! TAATTTAGAGTTCGATCGTG+CGG + chr2_3:48178283-48178302 Msa0295570:intron 35.0%
! TAATTTAGAGTTCGATCGTG+CGG + chr2_3:48178283-48178302 Msa0295570:intron 35.0%
AAATTGTGGTGATTGAAGCA+AGG + chr2_3:48179308-48179327 Msa0295570:CDS 35.0%
CATTTGGCTGATACATATAG+AGG + chr2_3:48179340-48179359 Msa0295570:CDS 35.0%
AAACTGTTAAGTGTGGAATG+TGG + chr2_3:48179459-48179478 Msa0295570:exon 35.0%
ACGATATTGATGAGTACCTA+TGG - chr2_3:48179640-48179659 None:intergenic 35.0%
CATCAATACGTAGAAATGTG+TGG + chr2_3:48178102-48178121 Msa0295570:intron 35.0%
! TAATTTAGAGTTCGATCGTG+CGG + chr2_3:48178283-48178302 Msa0295570:intron 35.0%
! TAATTTAGAGTTCGATCGTG+CGG + chr2_3:48178283-48178302 Msa0295570:intron 35.0%
AAATTGTGGTGATTGAAGCA+AGG + chr2_3:48179308-48179327 Msa0295570:CDS 35.0%
CATTTGGCTGATACATATAG+AGG + chr2_3:48179340-48179359 Msa0295570:CDS 35.0%
AAACTGTTAAGTGTGGAATG+TGG + chr2_3:48179459-48179478 Msa0295570:exon 35.0%
ACGATATTGATGAGTACCTA+TGG - chr2_3:48179640-48179659 None:intergenic 35.0%
GACAAATTGAACCGTTTGGA+CGG + chr2_3:48178032-48178051 Msa0295570:intron 40.0%
! TTGTCTGAAATCCGTCCAAA+CGG - chr2_3:48178046-48178065 None:intergenic 40.0%
GACAAATTGAACCGTTTGGA+CGG + chr2_3:48178032-48178051 Msa0295570:intron 40.0%
! TTGTCTGAAATCCGTCCAAA+CGG - chr2_3:48178046-48178065 None:intergenic 40.0%
CATCAATGCGTAGAAATGTG+TGG + chr2_3:48178852-48178871 Msa0295570:intron 40.0%
CATGGATGCAGAAGAAATTG+TGG + chr2_3:48179294-48179313 Msa0295570:intron 40.0%
GCACTCAAAACTGTTAAGTG+TGG + chr2_3:48179452-48179471 Msa0295570:exon 40.0%
GACAAATTGAACCGTTTGGA+CGG + chr2_3:48178032-48178051 Msa0295570:intron 40.0%
! TTGTCTGAAATCCGTCCAAA+CGG - chr2_3:48178046-48178065 None:intergenic 40.0%
GACAAATTGAACCGTTTGGA+CGG + chr2_3:48178032-48178051 Msa0295570:intron 40.0%
! TTGTCTGAAATCCGTCCAAA+CGG - chr2_3:48178046-48178065 None:intergenic 40.0%
CATCAATGCGTAGAAATGTG+TGG + chr2_3:48178852-48178871 Msa0295570:intron 40.0%
CATGGATGCAGAAGAAATTG+TGG + chr2_3:48179294-48179313 Msa0295570:intron 40.0%
GCACTCAAAACTGTTAAGTG+TGG + chr2_3:48179452-48179471 Msa0295570:exon 40.0%
ATGGAGAAGAGATCAGTAGC+TGG + chr2_3:48177554-48177573 Msa0295570:CDS 45.0%
AGCAAGGACTTGTGAGCATT+TGG + chr2_3:48179324-48179343 Msa0295570:CDS 45.0%
! AGTTGCATGCATCTTGGTGT+TGG - chr2_3:48179497-48179516 None:intergenic 45.0%
CTTGCAAGTTGCATGCATCT+TGG - chr2_3:48179503-48179522 None:intergenic 45.0%
ATGGAGAAGAGATCAGTAGC+TGG + chr2_3:48177554-48177573 Msa0295570:CDS 45.0%
AGCAAGGACTTGTGAGCATT+TGG + chr2_3:48179324-48179343 Msa0295570:CDS 45.0%
! AGTTGCATGCATCTTGGTGT+TGG - chr2_3:48179497-48179516 None:intergenic 45.0%
CTTGCAAGTTGCATGCATCT+TGG - chr2_3:48179503-48179522 None:intergenic 45.0%
!!! GAATAAATTTTTTTTAAATA+GGG - chr2_3:48179256-48179275 None:intergenic 5.0%
!!! AGAATAAATTTTTTTTAAAT+AGG - chr2_3:48179257-48179276 None:intergenic 5.0%
!!! GAATAAATTTTTTTTAAATA+GGG - chr2_3:48179256-48179275 None:intergenic 5.0%
!!! AGAATAAATTTTTTTTAAAT+AGG - chr2_3:48179257-48179276 None:intergenic 5.0%
CGACAAAGAGAACGAGGAAG+AGG - chr2_3:48177589-48177608 None:intergenic 50.0%
TACGTGCGACAAAGAGAACG+AGG - chr2_3:48177595-48177614 None:intergenic 50.0%
CAGCTAGCATCTGTGAAGCA+TGG - chr2_3:48179367-48179386 None:intergenic 50.0%
CGACAAAGAGAACGAGGAAG+AGG - chr2_3:48177589-48177608 None:intergenic 50.0%
TACGTGCGACAAAGAGAACG+AGG - chr2_3:48177595-48177614 None:intergenic 50.0%
CAGCTAGCATCTGTGAAGCA+TGG - chr2_3:48179367-48179386 None:intergenic 50.0%
! GAACGAGGAAGAGGAAGCAC+AGG - chr2_3:48177580-48177599 None:intergenic 55.0%
! GAACGAGGAAGAGGAAGCAC+AGG - chr2_3:48177580-48177599 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 48177446 48179731 48177446 ID=Msa0295570;Name=Msa0295570
chr2_3 mRNA 48177446 48179731 48177446 ID=Msa0295570-mRNA-1;Parent=Msa0295570;Name=Msa0295570-mRNA-1;_AED=0.00;_eAED=0.00;_QI=108|1|1|1|0|0|2|263|73
chr2_3 exon 48177446 48177611 48177446 ID=Msa0295570-mRNA-1:exon:15020;Parent=Msa0295570-mRNA-1
chr2_3 exon 48179305 48179731 48179305 ID=Msa0295570-mRNA-1:exon:15021;Parent=Msa0295570-mRNA-1
chr2_3 five_prime_UTR 48177446 48177553 48177446 ID=Msa0295570-mRNA-1:five_prime_utr;Parent=Msa0295570-mRNA-1
chr2_3 CDS 48177554 48177611 48177554 ID=Msa0295570-mRNA-1:cds;Parent=Msa0295570-mRNA-1
chr2_3 CDS 48179305 48179468 48179305 ID=Msa0295570-mRNA-1:cds;Parent=Msa0295570-mRNA-1
chr2_3 three_prime_UTR 48179469 48179731 48179469 ID=Msa0295570-mRNA-1:three_prime_utr;Parent=Msa0295570-mRNA-1
Gene Sequence

>Msa0295570

ATGGAGAAGAGATCAGTAGCTGGCCTGTGCTTCCTCTTCCTCGTTCTCTTTGTCGCACAAGAAATTGTGGTGATTGAAGCAAGGACTTGTGAGCATTTGGCTGATACATATAGAGGACCATGCTTCACAGATGCTAGCTGTGACGATCACTGCAAGAACAAAGCACACTTAATAAGTGGCACGTGCCATAGATTTCAATGTTTCTGCACTCAAAACTGTTAA

Protein sequence

>Msa0295570

MEKRSVAGLCFLFLVLFVAQEIVVIEARTCEHLADTYRGPCFTDASCDDHCKNKAHLISGTCHRFQCFCTQNC*