AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0675130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0675130 MtrunA17_Chr4g0018391 83.662 355 17 3 1 323 1 346 0.0 603
Msa0675130 MtrunA17_Chr4g0056541 52.926 376 103 13 1 321 1 357 2.38e-115 338
Msa0675130 MtrunA17_Chr5g0398971 53.681 326 81 13 1 284 1 298 8.89e-105 310
Msa0675130 MtrunA17_Chr5g0405211 48.447 322 91 9 5 284 6 294 1.58e-76 238
Msa0675130 MtrunA17_Chr8g0372121 42.188 320 90 6 5 285 4 267 1.37e-74 232
Msa0675130 MtrunA17_Chr8g0346001 39.828 349 137 8 1 288 9 345 3.96e-67 216
Msa0675130 MtrunA17_Chr1g0200561 62.821 156 27 1 1 125 13 168 8.08e-64 205
Msa0675130 MtrunA17_Chr2g0309331 39.490 314 121 10 7 280 10 294 1.47e-60 197
Msa0675130 MtrunA17_Chr4g0017921 52.717 184 52 3 7 160 8 186 2.23e-58 191
Msa0675130 MtrunA17_Chr2g0309891 60.748 107 41 1 7 113 6 111 2.48e-42 149
Msa0675130 MtrunA17_Chr6g0481091 57.944 107 44 1 7 113 6 111 6.91e-41 142
Msa0675130 MtrunA17_Chr3g0109821 46.405 153 47 3 7 125 6 157 5.43e-40 148
Msa0675130 MtrunA17_Chr6g0484441 40.314 191 77 3 7 163 5 192 9.48e-40 142
Msa0675130 MtrunA17_Chr2g0316971 57.658 111 42 2 5 113 21 128 2.88e-39 142
Msa0675130 MtrunA17_Chr3g0091921 46.497 157 46 3 7 128 16 169 3.97e-39 140
Msa0675130 MtrunA17_Chr4g0060931 46.104 154 45 3 7 125 17 167 1.70e-38 139
Msa0675130 MtrunA17_Chr6g0453451 34.194 310 133 11 7 268 20 306 4.05e-38 137
Msa0675130 MtrunA17_Chr5g0433781 57.009 107 45 1 7 113 6 111 6.37e-38 142
Msa0675130 MtrunA17_Chr8g0361731 35.545 211 87 4 9 173 16 223 5.86e-37 136
Msa0675130 MtrunA17_Chr1g0183741 37.688 199 78 5 7 173 10 194 6.41e-37 133
Msa0675130 MtrunA17_Chr2g0312321 37.118 229 102 5 5 196 9 232 1.20e-36 133
Msa0675130 MtrunA17_Chr7g0252971 50.847 118 46 2 5 113 6 120 1.67e-36 134
Msa0675130 MtrunA17_Chr7g0217721 58.654 104 40 1 10 113 22 122 3.03e-36 133
Msa0675130 MtrunA17_Chr1g0147421 46.405 153 45 4 7 125 27 176 3.96e-36 133
Msa0675130 MtrunA17_Chr7g0253011 54.054 111 44 2 7 113 17 124 4.32e-36 134
Msa0675130 MtrunA17_Chr7g0217711 57.692 104 41 1 10 113 22 122 9.87e-36 127
Msa0675130 MtrunA17_Chr5g0400461 49.138 116 51 2 5 113 4 118 6.74e-35 129
Msa0675130 MtrunA17_Chr5g0417001 37.209 172 62 3 7 135 14 182 1.51e-34 130
Msa0675130 MtrunA17_Chr3g0130731 53.271 107 46 2 7 113 7 109 2.45e-34 127
Msa0675130 MtrunA17_Chr4g0042561 52.294 109 49 1 5 113 7 112 2.67e-34 126
Msa0675130 MtrunA17_Chr5g0417791 51.402 107 49 1 7 113 3 106 3.62e-34 125
Msa0675130 MtrunA17_Chr3g0139331 42.105 152 52 3 7 125 11 159 3.88e-34 127
Msa0675130 MtrunA17_Chr3g0144271 54.545 110 44 3 7 113 23 129 4.78e-34 129
Msa0675130 MtrunA17_Chr8g0384721 52.336 107 47 2 7 113 7 109 7.89e-34 125
Msa0675130 MtrunA17_Chr1g0197351 57.009 107 41 3 9 113 44 147 9.32e-34 129
Msa0675130 MtrunA17_Chr5g0428991 53.153 111 51 1 3 113 21 130 9.99e-34 130
Msa0675130 MtrunA17_Chr7g0261411 41.333 150 54 3 5 123 8 154 1.01e-33 125
Msa0675130 MtrunA17_Chr2g0318101 30.337 267 113 6 6 226 14 253 1.10e-33 127
Msa0675130 MtrunA17_Chr4g0054791 50.893 112 51 2 5 113 4 114 1.40e-33 125
Msa0675130 MtrunA17_Chr2g0311661 52.679 112 46 3 7 113 20 129 1.72e-33 124
Msa0675130 MtrunA17_Chr4g0047841 50.000 110 50 2 6 113 14 120 1.96e-33 125
Msa0675130 MtrunA17_Chr6g0461661 36.098 205 105 6 7 191 18 216 3.10e-33 126
Msa0675130 MtrunA17_Chr2g0311651 52.679 112 46 3 7 113 21 130 4.78e-33 124
Msa0675130 MtrunA17_Chr2g0326361 53.636 110 42 3 8 113 15 119 5.67e-33 124
Msa0675130 MtrunA17_Chr3g0124761 51.852 108 48 2 6 113 6 109 6.07e-33 123
Msa0675130 MtrunA17_Chr6g0453131 42.069 145 70 4 1 141 9 143 1.02e-32 125
Msa0675130 MtrunA17_Chr3g0128111 52.885 104 48 1 10 113 45 147 3.93e-32 120
Msa0675130 MtrunA17_Chr7g0214071 44.444 153 46 4 7 126 15 161 5.36e-32 120
Msa0675130 MtrunA17_Chr3g0128151 51.351 111 53 1 3 113 18 127 5.52e-32 125
Msa0675130 MtrunA17_Chr7g0263931 49.091 110 51 2 6 113 10 116 2.56e-31 120
Msa0675130 MtrunA17_Chr5g0442771 37.931 174 91 5 7 172 18 182 2.90e-31 119
Msa0675130 MtrunA17_Chr2g0318081 38.158 152 58 2 7 125 16 164 3.47e-31 120
Msa0675130 MtrunA17_Chr4g0076731 48.148 108 52 2 7 113 54 158 4.69e-31 121
Msa0675130 MtrunA17_Chr1g0201021 49.524 105 52 1 9 113 21 124 1.06e-30 121
Msa0675130 MtrunA17_Chr3g0092671 51.887 106 49 2 9 113 7 111 1.48e-30 120
Msa0675130 MtrunA17_Chr8g0359921 47.664 107 53 1 7 113 14 117 2.43e-30 117
Msa0675130 MtrunA17_Chr1g0197361 51.887 106 49 2 9 113 10 114 3.74e-30 120
Msa0675130 MtrunA17_Chr3g0130101 49.524 105 52 1 9 113 9 112 8.60e-30 116
Msa0675130 MtrunA17_Chr8g0364791 33.898 177 78 4 9 148 8 182 2.93e-28 113
Msa0675130 MtrunA17_Chr8g0384201 33.708 178 79 4 9 149 8 183 1.59e-27 112
Msa0675130 MtrunA17_Chr4g0038751 46.296 108 52 2 9 113 17 121 1.86e-27 108
Msa0675130 MtrunA17_Chr8g0345671 39.873 158 77 6 2 150 3 151 4.92e-26 107
Msa0675130 MtrunA17_Chr8g0385991 46.491 114 54 2 2 113 9 117 3.45e-25 101
Msa0675130 MtrunA17_Chr8g0345691 46.491 114 57 3 3 113 8 120 1.58e-24 102
Msa0675130 MtrunA17_Chr4g0026141 30.072 276 152 11 8 270 6 253 1.86e-24 100
Msa0675130 MtrunA17_Chr8g0345711 43.636 110 58 3 7 113 12 120 4.84e-22 91.3
Msa0675130 MtrunA17_Chr2g0321631 43.363 113 54 5 8 113 5 114 6.57e-22 92.8
Msa0675130 MtrunA17_Chr3g0131911 42.241 116 58 3 2 113 9 119 7.40e-22 92.4
Msa0675130 MtrunA17_Chr2g0321741 44.828 87 46 1 27 113 12 96 2.98e-19 87.0
Msa0675130 MtrunA17_Chr8g0345731 40.909 110 61 3 7 113 24 132 1.93e-18 81.3
Msa0675130 MtrunA17_Chr8g0345701 38.462 130 62 5 1 113 1 129 4.24e-18 80.5
Msa0675130 MtrunA17_Chr4g0050701 38.318 107 53 3 7 111 11 106 5.92e-17 77.8
Msa0675130 MtrunA17_Chr7g0266701 28.000 225 100 8 7 171 4 226 6.90e-17 79.0
Msa0675130 MtrunA17_Chr3g0113001 55.385 65 27 1 51 113 4 68 8.03e-17 79.3
Msa0675130 MtrunA17_Chr8g0384351 32.530 166 65 7 7 146 10 154 1.01e-14 71.6
Msa0675130 MtrunA17_Chr8g0345741 41.414 99 54 3 18 113 2 99 1.53e-13 67.4
Msa0675130 MtrunA17_Chr3g0113031 65.854 41 14 0 73 113 3 43 4.46e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0675130 AT1G12260.1 48.744 398 122 14 1 323 1 391 3.02e-105 313
Msa0675130 AT1G12260.2 47.814 366 120 12 1 323 1 338 2.43e-96 289
Msa0675130 AT1G62700.1 48.077 364 109 14 1 301 1 347 3.35e-95 288
Msa0675130 AT1G62700.2 48.077 364 109 14 1 301 1 347 3.35e-95 288
Msa0675130 AT4G36160.2 45.294 340 130 8 7 303 14 340 6.71e-82 253
Msa0675130 AT4G36160.1 45.294 340 130 8 7 303 10 336 7.17e-82 253
Msa0675130 AT4G36160.3 45.294 340 130 8 7 303 10 336 7.17e-82 253
Msa0675130 AT2G18060.3 47.164 335 107 13 7 298 9 316 9.53e-79 244
Msa0675130 AT2G18060.1 47.164 335 107 13 7 298 9 316 9.53e-79 244
Msa0675130 AT5G66300.1 44.828 319 98 7 7 285 12 292 2.44e-77 238
Msa0675130 AT5G62380.2 40.688 349 120 10 1 296 1 315 6.71e-75 234
Msa0675130 AT5G62380.1 40.688 349 120 10 1 296 1 315 6.71e-75 234
Msa0675130 AT2G46770.1 40.503 358 101 12 1 301 10 312 2.31e-67 215
Msa0675130 AT3G61910.1 38.806 335 102 7 1 289 5 282 1.79e-62 202
Msa0675130 AT1G71930.2 59.091 154 32 1 5 127 7 160 4.35e-61 198
Msa0675130 AT1G71930.1 59.091 154 32 1 5 127 7 160 4.35e-61 198
Msa0675130 AT1G32770.1 56.213 169 31 1 1 126 10 178 3.46e-58 191
Msa0675130 AT4G10350.1 36.825 315 147 7 7 288 9 304 8.32e-57 187
Msa0675130 AT1G79580.3 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0675130 AT1G79580.5 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0675130 AT1G79580.4 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0675130 AT1G79580.2 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0675130 AT1G79580.1 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0675130 AT1G33280.1 57.333 150 34 1 7 126 8 157 6.51e-56 184
Msa0675130 AT3G17730.1 46.199 171 75 5 7 171 6 165 3.28e-41 144
Msa0675130 AT3G18400.1 46.053 152 47 2 6 125 4 152 6.34e-40 142
Msa0675130 AT5G39610.1 44.910 167 54 3 7 138 20 183 2.10e-39 140
Msa0675130 AT1G54330.1 44.586 157 56 3 7 133 6 161 2.99e-39 140
Msa0675130 AT5G17260.1 45.806 155 49 3 7 127 6 159 4.39e-39 144
Msa0675130 AT3G03200.1 46.452 155 48 3 7 127 6 159 6.04e-39 144
Msa0675130 AT3G04060.1 32.886 298 119 10 7 263 20 277 1.18e-38 140
Msa0675130 AT2G24430.2 45.860 157 48 3 7 129 16 169 1.26e-38 139
Msa0675130 AT2G24430.1 45.860 157 48 3 7 129 16 169 1.26e-38 139
Msa0675130 AT1G65910.1 57.009 107 45 1 7 113 6 111 1.93e-38 144
Msa0675130 AT5G61430.1 45.455 154 46 3 7 125 16 166 3.35e-38 139
Msa0675130 AT3G29035.1 57.009 107 43 1 7 113 24 127 4.80e-38 137
Msa0675130 AT5G07680.2 57.009 107 43 1 7 113 3 106 1.87e-37 136
Msa0675130 AT3G12977.1 38.498 213 75 6 5 165 16 224 2.09e-37 135
Msa0675130 AT5G07680.1 57.009 107 43 1 7 113 17 120 2.71e-37 136
Msa0675130 AT1G76420.1 44.444 153 48 3 7 125 22 171 6.66e-37 135
Msa0675130 AT5G46590.2 54.545 110 48 2 5 113 4 112 9.89e-37 133
Msa0675130 AT5G46590.1 54.545 110 48 2 5 113 4 112 1.24e-36 133
Msa0675130 AT3G04070.2 54.386 114 44 2 5 113 8 118 2.55e-36 134
Msa0675130 AT1G69490.1 39.634 164 59 2 5 131 7 167 3.74e-36 131
Msa0675130 AT3G04070.1 54.386 114 44 2 5 113 8 118 3.81e-36 134
Msa0675130 AT3G15170.1 56.757 111 43 2 5 113 18 125 5.78e-36 132
Msa0675130 AT3G10490.1 55.046 109 48 1 5 113 25 132 1.20e-35 129
Msa0675130 AT4G35580.1 55.856 111 48 1 3 113 5 114 1.26e-35 135
Msa0675130 AT3G10480.2 56.075 107 46 1 7 113 27 132 1.46e-35 134
Msa0675130 AT3G10480.1 56.075 107 46 1 7 113 27 132 1.48e-35 134
Msa0675130 AT4G35580.3 55.856 111 48 1 3 113 5 114 1.51e-35 135
Msa0675130 AT4G35580.2 55.856 111 48 1 3 113 5 114 1.54e-35 135
Msa0675130 AT2G43000.1 40.000 155 55 3 9 129 20 170 1.79e-35 130
Msa0675130 AT4G17980.1 54.955 111 47 2 5 113 4 113 3.01e-35 129
Msa0675130 AT4G17980.2 54.955 111 47 2 5 113 4 113 5.76e-35 129
Msa0675130 AT1G26870.1 37.427 171 60 3 9 135 18 185 6.77e-35 131
Msa0675130 AT1G61110.1 52.294 109 48 2 6 113 15 120 7.99e-35 129
Msa0675130 AT5G18270.1 42.169 166 56 5 7 135 21 183 1.00e-34 129
Msa0675130 AT3G10490.2 57.143 105 44 1 9 113 29 132 1.04e-34 132
Msa0675130 AT5G18270.2 42.169 166 56 5 7 135 21 183 1.09e-34 129
Msa0675130 AT5G53950.1 54.545 110 45 2 6 113 16 122 1.51e-34 130
Msa0675130 AT1G33060.2 55.140 107 47 1 7 113 24 129 1.74e-34 133
Msa0675130 AT1G33060.1 55.140 107 47 1 7 113 24 129 1.96e-34 133
Msa0675130 AT5G63790.2 52.778 108 47 2 6 113 59 162 2.59e-34 128
Msa0675130 AT5G63790.1 52.778 108 47 2 6 113 49 152 3.09e-34 127
Msa0675130 AT1G56010.2 40.244 164 58 3 5 131 17 177 4.54e-34 127
Msa0675130 AT5G08790.1 51.327 113 51 2 1 113 1 109 1.28e-33 125
Msa0675130 AT5G04410.1 35.079 191 85 4 2 155 4 192 1.48e-33 130
Msa0675130 AT5G39820.1 50.459 109 49 2 7 113 20 125 2.18e-33 126
Msa0675130 AT3G10500.1 33.182 220 94 5 2 170 4 221 1.15e-32 127
Msa0675130 AT5G24590.2 52.679 112 51 2 3 113 9 119 1.55e-32 125
Msa0675130 AT1G01720.1 48.673 113 54 2 1 113 1 109 1.62e-32 122
Msa0675130 AT4G27410.2 39.474 152 56 2 7 125 14 162 1.63e-32 122
Msa0675130 AT3G49530.2 52.778 108 49 2 7 113 13 119 2.39e-32 124
Msa0675130 AT3G49530.1 52.778 108 49 2 7 113 13 119 3.56e-32 125
Msa0675130 AT1G77450.1 50.926 108 49 2 6 113 9 112 5.30e-32 120
Msa0675130 AT2G33480.2 40.789 152 53 2 6 126 14 159 2.90e-31 118
Msa0675130 AT2G33480.1 50.926 108 47 1 6 113 14 115 3.07e-31 118
Msa0675130 AT2G02450.2 48.148 108 52 2 7 113 51 155 4.37e-31 121
Msa0675130 AT2G02450.1 48.148 108 52 2 7 113 51 155 4.51e-31 120
Msa0675130 AT2G27300.1 46.789 109 56 1 5 113 12 118 6.73e-31 119
Msa0675130 AT1G32510.1 47.414 116 53 2 5 113 4 118 6.95e-31 118
Msa0675130 AT4G27410.3 46.281 121 51 2 7 113 14 134 1.12e-30 118
Msa0675130 AT3G10480.3 44.286 140 44 3 7 113 27 165 2.04e-30 120
Msa0675130 AT3G15500.1 36.538 156 63 2 7 129 14 166 3.66e-30 117
Msa0675130 AT4G28530.1 37.870 169 53 5 5 125 8 172 4.06e-30 117
Msa0675130 AT4G10350.2 31.852 270 132 7 52 288 7 257 4.36e-30 116
Msa0675130 AT4G28530.2 42.029 138 53 4 5 125 8 135 5.37e-30 116
Msa0675130 AT1G52890.1 36.538 156 63 2 7 129 14 166 1.61e-29 115
Msa0675130 AT1G52880.1 55.435 92 38 1 11 102 21 109 3.22e-29 114
Msa0675130 AT1G34180.3 45.714 105 56 1 9 113 18 121 6.18e-29 115
Msa0675130 AT1G34180.1 45.714 105 56 1 9 113 18 121 7.54e-29 116
Msa0675130 AT5G64060.1 28.470 281 143 7 5 247 4 264 1.08e-28 114
Msa0675130 AT3G15510.1 48.571 105 49 2 11 113 21 122 1.45e-28 113
Msa0675130 AT5G09330.5 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0675130 AT5G09330.2 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0675130 AT5G09330.4 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0675130 AT5G09330.3 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0675130 AT5G09330.1 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0675130 AT5G22290.1 44.037 109 59 1 5 113 19 125 6.77e-28 111
Msa0675130 AT3G44290.2 42.857 105 58 1 9 113 16 118 8.27e-28 108
Msa0675130 AT2G17040.1 45.370 108 53 2 9 113 8 112 9.05e-28 109
Msa0675130 AT5G13180.1 37.107 159 60 4 2 126 9 161 9.91e-28 108
Msa0675130 AT1G34190.1 46.667 105 55 1 9 113 18 121 1.35e-27 113
Msa0675130 AT3G44290.1 42.857 105 58 1 9 113 16 118 3.93e-27 109
Msa0675130 AT5G04395.1 45.082 122 54 3 5 113 26 147 5.60e-27 105
Msa0675130 AT1G34180.4 40.517 116 58 1 9 113 18 133 3.88e-26 108
Msa0675130 AT1G34180.2 40.517 116 58 1 9 113 18 133 3.95e-26 108
Msa0675130 AT3G12910.1 47.748 111 47 4 9 113 23 128 7.66e-26 105
Msa0675130 AT1G32870.1 45.794 107 55 2 7 113 10 113 4.21e-22 97.1
Msa0675130 AT1G32870.2 45.794 107 55 2 7 113 10 113 4.21e-22 97.1
Msa0675130 AT1G32870.3 45.794 107 55 2 7 113 46 149 4.53e-22 97.1
Msa0675130 AT3G12977.2 34.545 165 54 4 52 165 10 171 2.22e-20 88.6
Msa0675130 AT2G18060.2 34.855 241 91 12 88 285 2 219 7.00e-20 88.2
Msa0675130 AT1G02230.1 44.628 121 59 5 8 126 4 118 8.52e-20 89.4
Msa0675130 AT5G64530.2 41.284 109 51 3 7 113 3 100 1.84e-19 84.3
Msa0675130 AT5G22380.1 41.441 111 55 5 10 113 8 115 4.91e-19 85.1
Msa0675130 AT1G02250.1 45.378 119 57 5 8 124 4 116 5.59e-19 87.0
Msa0675130 AT5G64530.1 41.284 109 51 3 7 113 3 100 6.65e-19 83.6
Msa0675130 AT5G61430.2 43.878 98 20 2 63 125 6 103 1.77e-18 84.3
Msa0675130 AT1G56010.1 39.806 103 26 1 65 131 8 110 1.97e-17 80.9
Msa0675130 AT3G44350.1 38.182 110 59 5 10 113 8 114 5.00e-16 74.7
Msa0675130 AT3G44350.2 38.182 110 59 5 10 113 8 114 6.70e-16 76.3
Msa0675130 AT3G04420.2 40.367 109 60 3 8 113 4 110 1.70e-15 75.9
Msa0675130 AT4G01520.1 37.838 111 63 4 8 113 5 114 2.43e-15 75.9
Msa0675130 AT3G04420.1 40.367 109 60 3 8 113 4 110 3.32e-15 75.9
Msa0675130 AT4G01540.1 37.615 109 62 4 10 113 7 114 1.68e-14 74.3
Msa0675130 AT4G01540.3 37.615 109 62 4 10 113 7 114 1.74e-14 74.3
Msa0675130 AT4G01540.4 37.615 109 62 4 10 113 7 114 1.86e-14 73.9
Msa0675130 AT4G01540.2 37.615 109 62 4 10 113 7 114 2.03e-14 73.9
Msa0675130 AT4G01550.1 32.022 178 100 6 10 173 7 177 2.43e-14 73.9
Msa0675130 AT1G02220.1 40.367 109 62 3 7 113 3 110 4.58e-14 72.8
Msa0675130 AT2G33480.3 51.786 56 27 0 58 113 3 58 8.18e-13 67.0

Find 65 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATACTTCTTATCTTTA+TGG 0.078281 4_4:+23559177 None:intergenic
CAATATTGAATTCAGAAATT+TGG 0.171673 4_4:-23557487 Msa0675130:CDS
GAGCAACTGCAGCTGGATTT+TGG 0.212597 4_4:-23559136 Msa0675130:CDS
TGTTGTAACCATTGGATTAG+AGG 0.295684 4_4:+23557749 None:intergenic
TAATTGTTGGTATATCAAAT+TGG 0.305571 4_4:+23557893 None:intergenic
TAGTCAAAGGAAAAGGTCTT+TGG 0.307210 4_4:+23561023 None:intergenic
AATTGTCACGAGACATGTTT+TGG 0.317098 4_4:+23557829 None:intergenic
AATAATAACAACATCTTTCA+TGG 0.344913 4_4:-23557510 Msa0675130:CDS
TTGACTAATTAATTATATCT+TGG 0.350029 4_4:-23561007 Msa0675130:intron
GAAGAAGATTGAACAAGCTT+TGG 0.352286 4_4:+23557780 None:intergenic
AGTTTCATTCATGCAAGATA+TGG 0.353877 4_4:-23557940 Msa0675130:CDS
TTTATAGAGATTTGCAGAAT+AGG 0.359693 4_4:-23559233 Msa0675130:intron
TATAGTAAGCATAATTTGAT+AGG 0.379805 4_4:-23559086 Msa0675130:CDS
AAAGATAAGAAGTATCCAAC+TGG 0.410251 4_4:-23559173 Msa0675130:CDS
CTATTTGTTCTTGTCCCAGT+TGG 0.412869 4_4:+23559158 None:intergenic
CAAAACTTTCATGCTATGTA+TGG 0.416700 4_4:-23557642 Msa0675130:CDS
AGTCAAAGGAAAAGGTCTTT+GGG 0.417990 4_4:+23561024 None:intergenic
CAAACACAAGTTGGATATGA+TGG 0.442162 4_4:-23557425 None:intergenic
TCAAATTGGATTGAGAGGTT+TGG 0.444509 4_4:+23557907 None:intergenic
GTGTCATATGTTGTAACCAT+TGG 0.449797 4_4:+23557741 None:intergenic
ACAAATAGAGCAACTGCAGC+TGG 0.455592 4_4:-23559143 Msa0675130:CDS
GGAAGAAAATTGATTCAAGA+AGG 0.463332 4_4:-23559704 Msa0675130:CDS
AAATGAAAGCAAAGGCAAGC+TGG 0.470082 4_4:+23561166 None:intergenic
CCTCTACATCAAACACAAGT+TGG 0.475978 4_4:-23557434 Msa0675130:CDS
TTCCAATGTTGAACAAGGTA+TGG 0.483739 4_4:-23557613 Msa0675130:CDS
CCAACTTGTGTTTGATGTAG+AGG 0.486977 4_4:+23557434 None:intergenic
TTGGATTGAGAGGTTTGGTT+TGG 0.487041 4_4:+23557912 None:intergenic
CCTTTACAAAATTGAACCAT+GGG 0.489236 4_4:-23559652 Msa0675130:CDS
AACCATACCTTGTTCAACAT+TGG 0.489325 4_4:+23557611 None:intergenic
GTGAGCATGACTCTCCTTGT+TGG 0.496787 4_4:-23557974 Msa0675130:CDS
ATTGAACCATGGGATCTTGA+AGG 0.503378 4_4:-23559642 Msa0675130:intron
TTGATGATCAAGTTACTGAT+TGG 0.505048 4_4:-23557590 Msa0675130:CDS
GTTTGGAGCAGGAGGAAGGA+TGG 0.509198 4_4:-23558049 Msa0675130:intron
AACTTGTGGATTACTACCTT+AGG 0.520831 4_4:-23559725 Msa0675130:CDS
GATAGGAATGAGGAAAACAT+TGG 0.522127 4_4:-23559069 Msa0675130:intron
GACGGTTTGGAGCAGGAGGA+AGG 0.522417 4_4:-23558053 Msa0675130:intron
AATTAATTAGTCAAAGGAAA+AGG 0.523043 4_4:+23561016 None:intergenic
AAATTTGGAGAAACAAGAAA+TGG 0.525812 4_4:-23557472 Msa0675130:CDS
AAGAATATGATGGTAATTGT+TGG 0.533908 4_4:+23557880 None:intergenic
TGGGATGGAACCTAAACCCC+GGG 0.538292 4_4:+23559757 None:intergenic
TCCCACAGATGAGGAACTTG+TGG 0.541887 4_4:-23559739 Msa0675130:CDS
TTTGGAGCAGGAGGAAGGAT+GGG 0.544311 4_4:-23558048 Msa0675130:intron
AATGAAAGCAAAGGCAAGCT+GGG 0.545140 4_4:+23561167 None:intergenic
GTATATCAAATTGGATTGAG+AGG 0.548657 4_4:+23557902 None:intergenic
ATCCACAAGTTCCTCATCTG+TGG 0.551212 4_4:+23559737 None:intergenic
ACCTTTACAAAATTGAACCA+TGG 0.552414 4_4:-23559653 Msa0675130:CDS
ACAAGAAGAAATGAAAGCAA+AGG 0.555525 4_4:+23561158 None:intergenic
AGCATAATTTGATAGGAATG+AGG 0.556802 4_4:-23559079 Msa0675130:CDS
GAAGAACAAGTTATACAAAG+TGG 0.557940 4_4:-23557672 Msa0675130:CDS
CTTATACCTTCAAGATCCCA+TGG 0.563913 4_4:+23559636 None:intergenic
AAACATAACCTCTAATCCAA+TGG 0.567146 4_4:-23557757 Msa0675130:CDS
TCTTGTAAGAGCATTGATGA+AGG 0.569946 4_4:+23557693 None:intergenic
AGCTTTGGACTCTCTAGAAG+TGG 0.581594 4_4:+23557795 None:intergenic
CAAGTTCCTCATCTGTGGGA+TGG 0.581878 4_4:+23559742 None:intergenic
TCCACAAGTTCCTCATCTGT+GGG 0.587470 4_4:+23559738 None:intergenic
AGATATAATTAATTAGTCAA+AGG 0.591128 4_4:+23561010 None:intergenic
TAGGTTCCATCCCACAGATG+AGG 0.602397 4_4:-23559748 Msa0675130:CDS
AACAATTCCAATGTTGAACA+AGG 0.643045 4_4:-23557618 Msa0675130:CDS
GTGGGATGGAACCTAAACCC+CGG 0.647443 4_4:+23559756 None:intergenic
GAAAGCAACAGGAAGAGACA+AGG 0.657167 4_4:-23559114 Msa0675130:CDS
GAACCGAAGAACAAAATGAG+TGG 0.672469 4_4:-23559211 Msa0675130:CDS
AAGATAAGAAGTATCCAACT+GGG 0.681306 4_4:-23559172 Msa0675130:CDS
ACTTGATCTTCATACCAACA+AGG 0.708859 4_4:+23557960 None:intergenic
GGATGGAACCTAAACCCCGG+GGG 0.731147 4_4:+23559759 None:intergenic
GGGATGGAACCTAAACCCCG+GGG 0.785968 4_4:+23559758 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTCTTTTATGAAAATT+AGG - chr4_4:23558543-23558562 Msa0675130:intron 10.0%
!! TCAAAAAAGAAAAATAAAAA+AGG + chr4_4:23559372-23559391 None:intergenic 10.0%
!! AGATATAATTAATTAGTCAA+AGG + chr4_4:23557619-23557638 None:intergenic 15.0%
!! TTGACTAATTAATTATATCT+TGG - chr4_4:23557619-23557638 Msa0675130:CDS 15.0%
!!! TTTATACATCAACTAATTTT+TGG - chr4_4:23557743-23557762 Msa0675130:CDS 15.0%
!!! ATTTTCAAAATATTCTATGT+CGG - chr4_4:23558007-23558026 Msa0675130:CDS 15.0%
!! CTAAGAAAAATTTAAACTTA+GGG + chr4_4:23558180-23558199 None:intergenic 15.0%
!!! AAAATATTTTATAGAGAAAG+AGG - chr4_4:23558628-23558647 Msa0675130:intron 15.0%
!! TATTTGATATATGCTTAATT+TGG - chr4_4:23558734-23558753 Msa0675130:intron 15.0%
!! AATTAGAAAAGTTAATTGAT+AGG + chr4_4:23558787-23558806 None:intergenic 15.0%
!! TCTAAATATATGTTTATAAG+TGG + chr4_4:23560101-23560120 None:intergenic 15.0%
!! AATTAATTAGTCAAAGGAAA+AGG + chr4_4:23557613-23557632 None:intergenic 20.0%
!! CTAATTAATTATATCTTGGT+TGG - chr4_4:23557623-23557642 Msa0675130:CDS 20.0%
!! TAATTTAGTGAAAGAATTAG+AGG + chr4_4:23557869-23557888 None:intergenic 20.0%
!! CCTAAGAAAAATTTAAACTT+AGG + chr4_4:23558181-23558200 None:intergenic 20.0%
!!! CCTAAGTTTAAATTTTTCTT+AGG - chr4_4:23558178-23558197 Msa0675130:intron 20.0%
!! GAATAATGTTAGACAAAATA+AGG - chr4_4:23558200-23558219 Msa0675130:intron 20.0%
!! TGATATATGACATATATACA+TGG + chr4_4:23558403-23558422 None:intergenic 20.0%
!! CATATATCATGAAACAATAT+TGG - chr4_4:23558414-23558433 Msa0675130:intron 20.0%
!! TTTAAAGAATGTCAAATTGT+GGG + chr4_4:23558472-23558491 None:intergenic 20.0%
!! ATTTAAAGAATGTCAAATTG+TGG + chr4_4:23558473-23558492 None:intergenic 20.0%
!! ACATTCTTTAAATTCTTGTA+AGG - chr4_4:23558480-23558499 Msa0675130:intron 20.0%
!! CATTCTTTAAATTCTTGTAA+GGG - chr4_4:23558481-23558500 Msa0675130:intron 20.0%
!!! TGCTTGTTGAATAAAAAAAA+AGG + chr4_4:23558605-23558624 None:intergenic 20.0%
!! TCAATGAATAAAGTGATATT+TGG + chr4_4:23558760-23558779 None:intergenic 20.0%
!!! AATTTGATATATGTGTTTTC+AGG - chr4_4:23558803-23558822 Msa0675130:intron 20.0%
!! TTTGGATTAATATCAAAGTT+TGG + chr4_4:23559066-23559085 None:intergenic 20.0%
!!! AAATAGTGAACTTTTTTTGT+GGG + chr4_4:23559206-23559225 None:intergenic 20.0%
!!! TAAATAGTGAACTTTTTTTG+TGG + chr4_4:23559207-23559226 None:intergenic 20.0%
!! AAAATAATTTGATACTGAAG+CGG + chr4_4:23559234-23559253 None:intergenic 20.0%
!!! TTATTTTTCAGTTTCTTAGT+AGG - chr4_4:23559247-23559266 Msa0675130:intron 20.0%
!! TATAGTAAGCATAATTTGAT+AGG - chr4_4:23559540-23559559 Msa0675130:intron 20.0%
!!! AAAACATTGGTGTTTTATAA+AGG - chr4_4:23559570-23559589 Msa0675130:intron 20.0%
!! TCTCTAGATTTATTTAATGT+TGG - chr4_4:23559694-23559713 Msa0675130:CDS 20.0%
!!! GTAATTCTCATTTTACAAAT+CGG + chr4_4:23560075-23560094 None:intergenic 20.0%
!!! ATGTTATAGATTAGTTTGTA+CGG - chr4_4:23560378-23560397 Msa0675130:intron 20.0%
!!! TTTGTTTTTGTTATTTTCGA+CGG - chr4_4:23560555-23560574 Msa0675130:intron 20.0%
!! TAATTGTTGGTATATCAAAT+TGG + chr4_4:23560736-23560755 None:intergenic 20.0%
!! AATAATAACAACATCTTTCA+TGG - chr4_4:23561116-23561135 Msa0675130:five_prime_UTR 20.0%
!! CAATATTGAATTCAGAAATT+TGG - chr4_4:23561139-23561158 Msa0675130:five_prime_UTR 20.0%
!! TAACATTGTTGTGTTTTTCT+TGG - chr4_4:23557532-23557551 Msa0675130:CDS 25.0%
!! AACATTGTTGTGTTTTTCTT+GGG - chr4_4:23557533-23557552 Msa0675130:CDS 25.0%
!!! ATACATCAACTAATTTTTGG+AGG - chr4_4:23557746-23557765 Msa0675130:CDS 25.0%
! AAAGAAATTTCAGCACAAAA+AGG - chr4_4:23558078-23558097 Msa0675130:intron 25.0%
!!! TTTTTTATCCTTTAGAAGCA+TGG - chr4_4:23558236-23558255 Msa0675130:intron 25.0%
! AGCATATATCAAATAGACTT+TGG + chr4_4:23558729-23558748 None:intergenic 25.0%
!!! TTTTCAGGATTATAAAAGAG+AGG - chr4_4:23558818-23558837 Msa0675130:intron 25.0%
!! TTGAATCAATTTTCTTCCTA+AGG + chr4_4:23558920-23558939 None:intergenic 25.0%
! TTTATAGAGATTTGCAGAAT+AGG - chr4_4:23559393-23559412 Msa0675130:intron 25.0%
! TTGGATACTTCTTATCTTTA+TGG + chr4_4:23559452-23559471 None:intergenic 25.0%
!!! TTTACAAATCGGTTTTGTAA+TGG + chr4_4:23560064-23560083 None:intergenic 25.0%
! GCAATAGATAAGTCTAATAT+TGG + chr4_4:23560135-23560154 None:intergenic 25.0%
! AGTTGAAATCGTAATTTCTA+TGG - chr4_4:23560195-23560214 Msa0675130:intron 25.0%
! CATATACACAACAAAATATG+AGG - chr4_4:23560222-23560241 Msa0675130:intron 25.0%
!! AACAATAACAATTAGGGTTT+TGG + chr4_4:23560298-23560317 None:intergenic 25.0%
! ATCGACAACAATAACAATTA+GGG + chr4_4:23560304-23560323 None:intergenic 25.0%
!!! TGTTATAGATTAGTTTGTAC+GGG - chr4_4:23560379-23560398 Msa0675130:intron 25.0%
!!! TAACAAATGTTAGTCTTCTA+AGG + chr4_4:23560494-23560513 None:intergenic 25.0%
! AAGAATATGATGGTAATTGT+TGG + chr4_4:23560749-23560768 None:intergenic 25.0%
!!! TCTTTTTTGCAAGAATATGA+TGG + chr4_4:23560759-23560778 None:intergenic 25.0%
! AAATTTGGAGAAACAAGAAA+TGG - chr4_4:23561154-23561173 Msa0675130:five_prime_UTR 25.0%
ACAAGAAGAAATGAAAGCAA+AGG + chr4_4:23557471-23557490 None:intergenic 30.0%
AAACAGACAAAAAAGTTGAG+AGG - chr4_4:23557571-23557590 Msa0675130:CDS 30.0%
AACAGACAAAAAAGTTGAGA+GGG - chr4_4:23557572-23557591 Msa0675130:CDS 30.0%
! TTTTTGCAAGATCAACAATG+TGG + chr4_4:23558061-23558080 None:intergenic 30.0%
!! CTTAGGGTTTTTAGAAAATG+TGG + chr4_4:23558164-23558183 None:intergenic 30.0%
ATTTATCACCATGCTTCTAA+AGG + chr4_4:23558247-23558266 None:intergenic 30.0%
AAGCATGGTGATAAATAAGT+GGG - chr4_4:23558251-23558270 Msa0675130:intron 30.0%
! GGAAGAAAATTGATTCAAGA+AGG - chr4_4:23558922-23558941 Msa0675130:intron 30.0%
ACCTTTACAAAATTGAACCA+TGG - chr4_4:23558973-23558992 Msa0675130:intron 30.0%
CCTTTACAAAATTGAACCAT+GGG - chr4_4:23558974-23558993 Msa0675130:intron 30.0%
! TATCAAAGTTTGGAGCATAA+TGG + chr4_4:23559056-23559075 None:intergenic 30.0%
GATTGTATTTATGTGCAGTT+TGG + chr4_4:23559084-23559103 None:intergenic 30.0%
GACATTACGTAATATATGCA+GGG - chr4_4:23559140-23559159 Msa0675130:CDS 30.0%
ACATTACGTAATATATGCAG+GGG - chr4_4:23559141-23559160 Msa0675130:CDS 30.0%
! ATACCACTCATTTTGTTCTT+CGG + chr4_4:23559421-23559440 None:intergenic 30.0%
!! AAAGATAAGAAGTATCCAAC+TGG - chr4_4:23559453-23559472 Msa0675130:intron 30.0%
!! AAGATAAGAAGTATCCAACT+GGG - chr4_4:23559454-23559473 Msa0675130:intron 30.0%
AGCATAATTTGATAGGAATG+AGG - chr4_4:23559547-23559566 Msa0675130:intron 30.0%
ATTGGATAATGCATGAGTAT+AGG - chr4_4:23559619-23559638 Msa0675130:intron 30.0%
GTGTTTATCCAACATTCATT+GGG + chr4_4:23560004-23560023 None:intergenic 30.0%
TGTGTTTATCCAACATTCAT+TGG + chr4_4:23560005-23560024 None:intergenic 30.0%
ACAAATATCATCTTCGTGTT+TGG - chr4_4:23560022-23560041 Msa0675130:intron 30.0%
GATCGACAACAATAACAATT+AGG + chr4_4:23560305-23560324 None:intergenic 30.0%
GATAAAGTTGTGTTAACTCT+TGG - chr4_4:23560336-23560355 Msa0675130:intron 30.0%
!! AGACATTGAGAGTATTAAGA+AGG + chr4_4:23560449-23560468 None:intergenic 30.0%
!!! TTTTGTTATTTTCGACGGTT+TGG - chr4_4:23560560-23560579 Msa0675130:intron 30.0%
AGTTTCATTCATGCAAGATA+TGG - chr4_4:23560686-23560705 Msa0675130:intron 30.0%
GTATATCAAATTGGATTGAG+AGG + chr4_4:23560727-23560746 None:intergenic 30.0%
AAACATAACCTCTAATCCAA+TGG - chr4_4:23560869-23560888 Msa0675130:five_prime_UTR 30.0%
GAAGAACAAGTTATACAAAG+TGG - chr4_4:23560954-23560973 Msa0675130:intron 30.0%
CAAAACTTTCATGCTATGTA+TGG - chr4_4:23560984-23561003 Msa0675130:intron 30.0%
AACAATTCCAATGTTGAACA+AGG - chr4_4:23561008-23561027 Msa0675130:five_prime_UTR 30.0%
! TTGATGATCAAGTTACTGAT+TGG - chr4_4:23561036-23561055 Msa0675130:five_prime_UTR 30.0%
AGTCAAAGGAAAAGGTCTTT+GGG + chr4_4:23557605-23557624 None:intergenic 35.0%
TAGTCAAAGGAAAAGGTCTT+TGG + chr4_4:23557606-23557625 None:intergenic 35.0%
! ACTTGACTCTTGACACTTTT+TGG - chr4_4:23557779-23557798 Msa0675130:CDS 35.0%
TCAGCACAAAAAGGAAAGAT+AGG - chr4_4:23558087-23558106 Msa0675130:intron 35.0%
!! AGGGTTTTTAGAAAATGTGG+AGG + chr4_4:23558161-23558180 None:intergenic 35.0%
GAAGCATGGTGATAAATAAG+TGG - chr4_4:23558250-23558269 Msa0675130:intron 35.0%
GAACATGATGATTATGCATG+TGG + chr4_4:23558369-23558388 None:intergenic 35.0%
CAAATTGTGGGATCAAAATG+TGG + chr4_4:23558460-23558479 None:intergenic 35.0%
! AACTTGTGGATTACTACCTT+AGG - chr4_4:23558901-23558920 Msa0675130:intron 35.0%
! CCCATGGTTCAATTTTGTAA+AGG + chr4_4:23558977-23558996 None:intergenic 35.0%
GGACATTACGTAATATATGC+AGG - chr4_4:23559139-23559158 Msa0675130:CDS 35.0%
TGTGGGACTACAATATAAGT+GGG + chr4_4:23559189-23559208 None:intergenic 35.0%
TTGTGGGACTACAATATAAG+TGG + chr4_4:23559190-23559209 None:intergenic 35.0%
GATAGGAATGAGGAAAACAT+TGG - chr4_4:23559557-23559576 Msa0675130:intron 35.0%
! CAATCTGACTTTTGTCCATT+GGG + chr4_4:23559603-23559622 None:intergenic 35.0%
AGGCTTGAAACAGATGAAAA+TGG - chr4_4:23559639-23559658 Msa0675130:intron 35.0%
! AAGAGTTTCGCCAAAAGTAT+CGG - chr4_4:23559781-23559800 Msa0675130:CDS 35.0%
! TGGTTTCAAACCGATACTTT+TGG + chr4_4:23559794-23559813 None:intergenic 35.0%
! CTTACAAGTTCACCTTTTAG+GGG + chr4_4:23559836-23559855 None:intergenic 35.0%
! CCTTACAAGTTCACCTTTTA+GGG + chr4_4:23559837-23559856 None:intergenic 35.0%
! ACCTTACAAGTTCACCTTTT+AGG + chr4_4:23559838-23559857 None:intergenic 35.0%
ATGAGGCAAAATAAACCTAG+AGG - chr4_4:23560239-23560258 Msa0675130:intron 35.0%
ACTTGATCTTCATACCAACA+AGG + chr4_4:23560669-23560688 None:intergenic 35.0%
TCAAATTGGATTGAGAGGTT+TGG + chr4_4:23560722-23560741 None:intergenic 35.0%
! AATTGTCACGAGACATGTTT+TGG + chr4_4:23560800-23560819 None:intergenic 35.0%
GAAGAAGATTGAACAAGCTT+TGG + chr4_4:23560849-23560868 None:intergenic 35.0%
! TGTTGTAACCATTGGATTAG+AGG + chr4_4:23560880-23560899 None:intergenic 35.0%
GTGTCATATGTTGTAACCAT+TGG + chr4_4:23560888-23560907 None:intergenic 35.0%
!! TCTTGTAAGAGCATTGATGA+AGG + chr4_4:23560936-23560955 None:intergenic 35.0%
TTCCAATGTTGAACAAGGTA+TGG - chr4_4:23561013-23561032 Msa0675130:five_prime_UTR 35.0%
AACCATACCTTGTTCAACAT+TGG + chr4_4:23561018-23561037 None:intergenic 35.0%
AATGAAAGCAAAGGCAAGCT+GGG + chr4_4:23557462-23557481 None:intergenic 40.0%
AAATGAAAGCAAAGGCAAGC+TGG + chr4_4:23557463-23557482 None:intergenic 40.0%
!! TGACTCTTGACACTTTTTGG+AGG - chr4_4:23557782-23557801 Msa0675130:CDS 40.0%
!!! TACTTTTTCTCATGTTCCCC+CGG - chr4_4:23558851-23558870 Msa0675130:intron 40.0%
ATTGAACCATGGGATCTTGA+AGG - chr4_4:23558984-23559003 Msa0675130:intron 40.0%
CTTATACCTTCAAGATCCCA+TGG + chr4_4:23558993-23559012 None:intergenic 40.0%
GAGCATAATGGCAACAACAA+CGG + chr4_4:23559044-23559063 None:intergenic 40.0%
TCTCATGTGTGTAGCTCATT+TGG - chr4_4:23559113-23559132 Msa0675130:CDS 40.0%
!! CAATATAAGTGGGTTGTCCA+GGG + chr4_4:23559179-23559198 None:intergenic 40.0%
!! ACAATATAAGTGGGTTGTCC+AGG + chr4_4:23559180-23559199 None:intergenic 40.0%
GAACCGAAGAACAAAATGAG+TGG - chr4_4:23559415-23559434 Msa0675130:intron 40.0%
CTATTTGTTCTTGTCCCAGT+TGG + chr4_4:23559471-23559490 None:intergenic 40.0%
! CCAATCTGACTTTTGTCCAT+TGG + chr4_4:23559604-23559623 None:intergenic 40.0%
CCAATGGACAAAAGTCAGAT+TGG - chr4_4:23559601-23559620 Msa0675130:intron 40.0%
!!! AGTTTTGAATCACTTGCCTG+TGG + chr4_4:23559669-23559688 None:intergenic 40.0%
!! AAGTTCACCTTTTAGGGGTT+GGG + chr4_4:23559831-23559850 None:intergenic 40.0%
CCCTAAAAGGTGAACTTGTA+AGG - chr4_4:23559834-23559853 Msa0675130:intron 40.0%
AAGAGTGACCCAATGAATGT+TGG - chr4_4:23559993-23560012 Msa0675130:intron 40.0%
GGAAAAAGTTCCAAGACTCT+TGG + chr4_4:23560277-23560296 None:intergenic 40.0%
TAAGGCGTAGTACAATGGTA+TGG + chr4_4:23560476-23560495 None:intergenic 40.0%
!! TCTTCTAAGGCGTAGTACAA+TGG + chr4_4:23560481-23560500 None:intergenic 40.0%
! TTGGATTGAGAGGTTTGGTT+TGG + chr4_4:23560717-23560736 None:intergenic 40.0%
!!! CACGAGACATGTTTTGGAAT+GGG + chr4_4:23560794-23560813 None:intergenic 40.0%
!!! TCACGAGACATGTTTTGGAA+TGG + chr4_4:23560795-23560814 None:intergenic 40.0%
!!! GATGTAGAGGCATTTTCATG+AGG + chr4_4:23561182-23561201 None:intergenic 40.0%
CCAACTTGTGTTTGATGTAG+AGG + chr4_4:23561195-23561214 None:intergenic 40.0%
CCTCTACATCAAACACAAGT+TGG - chr4_4:23561192-23561211 Msa0675130:five_prime_UTR 40.0%
!! ACTTTTTCTCATGTTCCCCC+GGG - chr4_4:23558852-23558871 Msa0675130:intron 45.0%
TCCACAAGTTCCTCATCTGT+GGG + chr4_4:23558891-23558910 None:intergenic 45.0%
ATCCACAAGTTCCTCATCTG+TGG + chr4_4:23558892-23558911 None:intergenic 45.0%
TGTGTGTAGCTCATTTGGCA+TGG - chr4_4:23559118-23559137 Msa0675130:CDS 45.0%
ACAAATAGAGCAACTGCAGC+TGG - chr4_4:23559483-23559502 Msa0675130:intron 45.0%
!! GCTGGATTTTGGAAAGCAAC+AGG - chr4_4:23559501-23559520 Msa0675130:intron 45.0%
GAAAGCAACAGGAAGAGACA+AGG - chr4_4:23559512-23559531 Msa0675130:intron 45.0%
TATAAAGGTCGAGCTCCCAA+TGG - chr4_4:23559585-23559604 Msa0675130:intron 45.0%
AGATGAAAATGGCACACCAC+AGG - chr4_4:23559650-23559669 Msa0675130:CDS 45.0%
!! CAAGTTCACCTTTTAGGGGT+TGG + chr4_4:23559832-23559851 None:intergenic 45.0%
CATTCCAAACGTCCAACCTT+AGG + chr4_4:23559940-23559959 None:intergenic 45.0%
!! GTTTGGAATGCGACTTGGTT+GGG - chr4_4:23559950-23559969 Msa0675130:intron 45.0%
!! TTGGGCTAGTACTGGTAACA+TGG - chr4_4:23559968-23559987 Msa0675130:intron 45.0%
!! TGGGCTAGTACTGGTAACAT+GGG - chr4_4:23559969-23559988 Msa0675130:intron 45.0%
AAGTTCCAAGACTCTTGGCT+TGG + chr4_4:23560272-23560291 None:intergenic 45.0%
!! ATTAGTTTGTACGGGTCGAG+AGG - chr4_4:23560387-23560406 Msa0675130:intron 45.0%
!! TATTTTCGACGGTTTGGAGC+AGG - chr4_4:23560566-23560585 Msa0675130:intron 45.0%
!! AGCTTTGGACTCTCTAGAAG+TGG + chr4_4:23560834-23560853 None:intergenic 45.0%
! CTTTTTCTCATGTTCCCCCG+GGG - chr4_4:23558853-23558872 Msa0675130:intron 50.0%
TAGGTTCCATCCCACAGATG+AGG - chr4_4:23558878-23558897 Msa0675130:intron 50.0%
CAAGTTCCTCATCTGTGGGA+TGG + chr4_4:23558887-23558906 None:intergenic 50.0%
TCCCACAGATGAGGAACTTG+TGG - chr4_4:23558887-23558906 Msa0675130:intron 50.0%
CGTAATATATGCAGGGGTCG+TGG - chr4_4:23559147-23559166 Msa0675130:CDS 50.0%
! GAGCAACTGCAGCTGGATTT+TGG - chr4_4:23559490-23559509 Msa0675130:intron 50.0%
! GTTGGGTTAGGCTCAACTCA+TGG + chr4_4:23559814-23559833 None:intergenic 50.0%
AGCCTAACCCAACCCCTAAA+AGG - chr4_4:23559821-23559840 Msa0675130:intron 50.0%
!! CACCTTTTAGGGGTTGGGTT+AGG + chr4_4:23559826-23559845 None:intergenic 50.0%
! TGGACGTTTGGAATGCGACT+TGG - chr4_4:23559945-23559964 Msa0675130:intron 50.0%
!! CGTTTGGAATGCGACTTGGT+TGG - chr4_4:23559949-23559968 Msa0675130:intron 50.0%
! TGGCTTGGACTACATCCTCT+AGG + chr4_4:23560257-23560276 None:intergenic 50.0%
GTAGTCCAAGCCAAGAGTCT+TGG - chr4_4:23560264-23560283 Msa0675130:intron 50.0%
! TTTGGAGCAGGAGGAAGGAT+GGG - chr4_4:23560578-23560597 Msa0675130:intron 50.0%
GTGAGCATGACTCTCCTTGT+TGG - chr4_4:23560652-23560671 Msa0675130:intron 50.0%
TGGGATGGAACCTAAACCCC+GGG + chr4_4:23558872-23558891 None:intergenic 55.0%
GTGGGATGGAACCTAAACCC+CGG + chr4_4:23558873-23558892 None:intergenic 55.0%
GCGTGAGTGGTGTGTGTTGA+AGG + chr4_4:23559918-23559937 None:intergenic 55.0%
ACACACACCACTCACGCCTA+AGG - chr4_4:23559921-23559940 Msa0675130:intron 55.0%
ACACCACTCACGCCTAAGGT+TGG - chr4_4:23559925-23559944 Msa0675130:intron 55.0%
CACGCCTAAGGTTGGACGTT+TGG - chr4_4:23559933-23559952 Msa0675130:intron 55.0%
!! CGACTTGGTTGGGCTAGTAC+TGG - chr4_4:23559960-23559979 Msa0675130:intron 55.0%
! TTTCGACGGTTTGGAGCAGG+AGG - chr4_4:23560569-23560588 Msa0675130:intron 55.0%
! GTTTGGAGCAGGAGGAAGGA+TGG - chr4_4:23560577-23560596 Msa0675130:intron 55.0%
CTCATGTTCCCCCGGGGTTT+AGG - chr4_4:23558859-23558878 Msa0675130:intron 60.0%
GGATGGAACCTAAACCCCGG+GGG + chr4_4:23558870-23558889 None:intergenic 60.0%
GGGATGGAACCTAAACCCCG+GGG + chr4_4:23558871-23558890 None:intergenic 60.0%
CGTCCAACCTTAGGCGTGAG+TGG + chr4_4:23559931-23559950 None:intergenic 60.0%
! GACGGTTTGGAGCAGGAGGA+AGG - chr4_4:23560573-23560592 Msa0675130:intron 60.0%
AGGGGTCGTGGTTCGAACCC+TGG - chr4_4:23559159-23559178 Msa0675130:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 23557428 23561220 23557428 ID=Msa0675130;Name=Msa0675130
chr4_4 mRNA 23557428 23561220 23557428 ID=Msa0675130-mRNA-1;Parent=Msa0675130;Name=Msa0675130-mRNA-1;_AED=0.09;_eAED=0.09;_QI=326|0.75|0.6|1|0.25|0.4|5|0|323
chr4_4 exon 23557428 23558060 23557428 ID=Msa0675130-mRNA-1:exon:6565;Parent=Msa0675130-mRNA-1
chr4_4 exon 23559070 23559248 23559070 ID=Msa0675130-mRNA-1:exon:6564;Parent=Msa0675130-mRNA-1
chr4_4 exon 23559643 23559823 23559643 ID=Msa0675130-mRNA-1:exon:6563;Parent=Msa0675130-mRNA-1
chr4_4 exon 23560814 23560905 23560814 ID=Msa0675130-mRNA-1:exon:6562;Parent=Msa0675130-mRNA-1
chr4_4 exon 23561008 23561220 23561008 ID=Msa0675130-mRNA-1:exon:6561;Parent=Msa0675130-mRNA-1
chr4_4 five_prime_UTR 23561008 23561220 23561008 ID=Msa0675130-mRNA-1:five_prime_utr;Parent=Msa0675130-mRNA-1
chr4_4 five_prime_UTR 23560814 23560905 23560814 ID=Msa0675130-mRNA-1:five_prime_utr;Parent=Msa0675130-mRNA-1
chr4_4 five_prime_UTR 23559803 23559823 23559803 ID=Msa0675130-mRNA-1:five_prime_utr;Parent=Msa0675130-mRNA-1
chr4_4 CDS 23559643 23559802 23559643 ID=Msa0675130-mRNA-1:cds;Parent=Msa0675130-mRNA-1
chr4_4 CDS 23559070 23559248 23559070 ID=Msa0675130-mRNA-1:cds;Parent=Msa0675130-mRNA-1
chr4_4 CDS 23557428 23558060 23557428 ID=Msa0675130-mRNA-1:cds;Parent=Msa0675130-mRNA-1
Gene Sequence

>Msa0675130

ATGAATACTTTTTCTCATGTTCCCCCGGGGTTTAGGTTCCATCCCACAGATGAGGAACTTGTGGATTACTACCTTAGGAAGAAAATTGATTCAAGAAGGATTGATCTTGATGTCATAAGAGATGTTGACCTTTACAAAATTGAACCATGGGATCTTGAAGAGATTTGCAGAATAGGAACCGAAGAACAAAATGAGTGGTATTTTTTTAGCCATAAAGATAAGAAGTATCCAACTGGGACAAGAACAAATAGAGCAACTGCAGCTGGATTTTGGAAAGCAACAGGAAGAGACAAGGCAATCTATAGTAAGCATAATTTGATAGGAATGAGGAAAACATTGGAGGAAGGATGGGTTGTATGTAGAGTATTCAAGAAGAGAGTAACAACAACAATTCGCAAAATGAGTGAGCATGACTCTCCTTGTTGGTATGAAGATCAAGTTTCATTCATGCAAGATATGGACTCACCAAACCAAACCTCTCAATCCAATTTGATATACCAACAATTACCATCATATTCTTGCAAAAAAGAGCTAGATAATTTCCCATTCCAAAACATGTCTCGTGACAATTATCTTAATCTTCCACTTCTAGAGAGTCCAAAGCTTGTTCAATCTTCTTCAAACATAACCTCTAATCCAATGGTTACAACATATGACACTATAGATCATTCAAATTCTATGCTTCCTTCATCAATGCTCTTACAAGAAGAACAAGTTATACAAAGTGGTAATCAACAAAACTTTCATGCTATGTATGGAAACAATTCCAATGTTGAACAAGGTATGGTTGATGATCAAGTTACTGATTGGAGAGTTCTTGACAAGTTTGTTGCTTCACAACTTAGTCAAGAAGATGCATCTAAAGTGAATAATAACAACATCTTTCATGGCAATATTGAATTCAGAAATTTGGAGAAACAAGAAATGGTGCCTCATGAAAATGCCTCTACATCAAACACAAGTTGGATATGA
Protein sequence

>Msa0675130

MNTFSHVPPGFRFHPTDEELVDYYLRKKIDSRRIDLDVIRDVDLYKIEPWDLEEICRIGTEEQNEWYFFSHKDKKYPTGTRTNRATAAGFWKATGRDKAIYSKHNLIGMRKTLEEGWVVCRVFKKRVTTTIRKMSEHDSPCWYEDQVSFMQDMDSPNQTSQSNLIYQQLPSYSCKKELDNFPFQNMSRDNYLNLPLLESPKLVQSSSNITSNPMVTTYDTIDHSNSMLPSSMLLQEEQVIQSGNQQNFHAMYGNNSNVEQGMVDDQVTDWRVLDKFVASQLSQEDASKVNNNNIFHGNIEFRNLEKQEMVPHENASTSNTSWI*