AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0596610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0596610 MtrunA17_Chr4g0018391 83.662 355 17 3 1 323 1 346 0.0 603
Msa0596610 MtrunA17_Chr4g0056541 52.926 376 103 13 1 321 1 357 2.38e-115 338
Msa0596610 MtrunA17_Chr5g0398971 53.681 326 81 13 1 284 1 298 8.89e-105 310
Msa0596610 MtrunA17_Chr5g0405211 48.447 322 91 9 5 284 6 294 1.58e-76 238
Msa0596610 MtrunA17_Chr8g0372121 42.188 320 90 6 5 285 4 267 1.37e-74 232
Msa0596610 MtrunA17_Chr8g0346001 39.828 349 137 8 1 288 9 345 3.96e-67 216
Msa0596610 MtrunA17_Chr1g0200561 62.821 156 27 1 1 125 13 168 8.08e-64 205
Msa0596610 MtrunA17_Chr2g0309331 39.490 314 121 10 7 280 10 294 1.47e-60 197
Msa0596610 MtrunA17_Chr4g0017921 52.717 184 52 3 7 160 8 186 2.23e-58 191
Msa0596610 MtrunA17_Chr2g0309891 60.748 107 41 1 7 113 6 111 2.48e-42 149
Msa0596610 MtrunA17_Chr6g0481091 57.944 107 44 1 7 113 6 111 6.91e-41 142
Msa0596610 MtrunA17_Chr3g0109821 46.405 153 47 3 7 125 6 157 5.43e-40 148
Msa0596610 MtrunA17_Chr6g0484441 40.314 191 77 3 7 163 5 192 9.48e-40 142
Msa0596610 MtrunA17_Chr2g0316971 57.658 111 42 2 5 113 21 128 2.88e-39 142
Msa0596610 MtrunA17_Chr3g0091921 46.497 157 46 3 7 128 16 169 3.97e-39 140
Msa0596610 MtrunA17_Chr4g0060931 46.104 154 45 3 7 125 17 167 1.70e-38 139
Msa0596610 MtrunA17_Chr6g0453451 34.194 310 133 11 7 268 20 306 4.05e-38 137
Msa0596610 MtrunA17_Chr5g0433781 57.009 107 45 1 7 113 6 111 6.37e-38 142
Msa0596610 MtrunA17_Chr8g0361731 35.545 211 87 4 9 173 16 223 5.86e-37 136
Msa0596610 MtrunA17_Chr1g0183741 37.688 199 78 5 7 173 10 194 6.41e-37 133
Msa0596610 MtrunA17_Chr2g0312321 37.118 229 102 5 5 196 9 232 1.20e-36 133
Msa0596610 MtrunA17_Chr7g0252971 50.847 118 46 2 5 113 6 120 1.67e-36 134
Msa0596610 MtrunA17_Chr7g0217721 58.654 104 40 1 10 113 22 122 3.03e-36 133
Msa0596610 MtrunA17_Chr1g0147421 46.405 153 45 4 7 125 27 176 3.96e-36 133
Msa0596610 MtrunA17_Chr7g0253011 54.054 111 44 2 7 113 17 124 4.32e-36 134
Msa0596610 MtrunA17_Chr7g0217711 57.692 104 41 1 10 113 22 122 9.87e-36 127
Msa0596610 MtrunA17_Chr5g0400461 49.138 116 51 2 5 113 4 118 6.74e-35 129
Msa0596610 MtrunA17_Chr5g0417001 37.209 172 62 3 7 135 14 182 1.51e-34 130
Msa0596610 MtrunA17_Chr3g0130731 53.271 107 46 2 7 113 7 109 2.45e-34 127
Msa0596610 MtrunA17_Chr4g0042561 52.294 109 49 1 5 113 7 112 2.67e-34 126
Msa0596610 MtrunA17_Chr5g0417791 51.402 107 49 1 7 113 3 106 3.62e-34 125
Msa0596610 MtrunA17_Chr3g0139331 42.105 152 52 3 7 125 11 159 3.88e-34 127
Msa0596610 MtrunA17_Chr3g0144271 54.545 110 44 3 7 113 23 129 4.78e-34 129
Msa0596610 MtrunA17_Chr8g0384721 52.336 107 47 2 7 113 7 109 7.89e-34 125
Msa0596610 MtrunA17_Chr1g0197351 57.009 107 41 3 9 113 44 147 9.32e-34 129
Msa0596610 MtrunA17_Chr5g0428991 53.153 111 51 1 3 113 21 130 9.99e-34 130
Msa0596610 MtrunA17_Chr7g0261411 41.333 150 54 3 5 123 8 154 1.01e-33 125
Msa0596610 MtrunA17_Chr2g0318101 30.337 267 113 6 6 226 14 253 1.10e-33 127
Msa0596610 MtrunA17_Chr4g0054791 50.893 112 51 2 5 113 4 114 1.40e-33 125
Msa0596610 MtrunA17_Chr2g0311661 52.679 112 46 3 7 113 20 129 1.72e-33 124
Msa0596610 MtrunA17_Chr4g0047841 50.000 110 50 2 6 113 14 120 1.96e-33 125
Msa0596610 MtrunA17_Chr6g0461661 36.098 205 105 6 7 191 18 216 3.10e-33 126
Msa0596610 MtrunA17_Chr2g0311651 52.679 112 46 3 7 113 21 130 4.78e-33 124
Msa0596610 MtrunA17_Chr2g0326361 53.636 110 42 3 8 113 15 119 5.67e-33 124
Msa0596610 MtrunA17_Chr3g0124761 51.852 108 48 2 6 113 6 109 6.07e-33 123
Msa0596610 MtrunA17_Chr6g0453131 42.069 145 70 4 1 141 9 143 1.02e-32 125
Msa0596610 MtrunA17_Chr3g0128111 52.885 104 48 1 10 113 45 147 3.93e-32 120
Msa0596610 MtrunA17_Chr7g0214071 44.444 153 46 4 7 126 15 161 5.36e-32 120
Msa0596610 MtrunA17_Chr3g0128151 51.351 111 53 1 3 113 18 127 5.52e-32 125
Msa0596610 MtrunA17_Chr7g0263931 49.091 110 51 2 6 113 10 116 2.56e-31 120
Msa0596610 MtrunA17_Chr5g0442771 37.931 174 91 5 7 172 18 182 2.90e-31 119
Msa0596610 MtrunA17_Chr2g0318081 38.158 152 58 2 7 125 16 164 3.47e-31 120
Msa0596610 MtrunA17_Chr4g0076731 48.148 108 52 2 7 113 54 158 4.69e-31 121
Msa0596610 MtrunA17_Chr1g0201021 49.524 105 52 1 9 113 21 124 1.06e-30 121
Msa0596610 MtrunA17_Chr3g0092671 51.887 106 49 2 9 113 7 111 1.48e-30 120
Msa0596610 MtrunA17_Chr8g0359921 47.664 107 53 1 7 113 14 117 2.43e-30 117
Msa0596610 MtrunA17_Chr1g0197361 51.887 106 49 2 9 113 10 114 3.74e-30 120
Msa0596610 MtrunA17_Chr3g0130101 49.524 105 52 1 9 113 9 112 8.60e-30 116
Msa0596610 MtrunA17_Chr8g0364791 33.898 177 78 4 9 148 8 182 2.93e-28 113
Msa0596610 MtrunA17_Chr8g0384201 33.708 178 79 4 9 149 8 183 1.59e-27 112
Msa0596610 MtrunA17_Chr4g0038751 46.296 108 52 2 9 113 17 121 1.86e-27 108
Msa0596610 MtrunA17_Chr8g0345671 39.873 158 77 6 2 150 3 151 4.92e-26 107
Msa0596610 MtrunA17_Chr8g0385991 46.491 114 54 2 2 113 9 117 3.45e-25 101
Msa0596610 MtrunA17_Chr8g0345691 46.491 114 57 3 3 113 8 120 1.58e-24 102
Msa0596610 MtrunA17_Chr4g0026141 30.072 276 152 11 8 270 6 253 1.86e-24 100
Msa0596610 MtrunA17_Chr8g0345711 43.636 110 58 3 7 113 12 120 4.84e-22 91.3
Msa0596610 MtrunA17_Chr2g0321631 43.363 113 54 5 8 113 5 114 6.57e-22 92.8
Msa0596610 MtrunA17_Chr3g0131911 42.241 116 58 3 2 113 9 119 7.40e-22 92.4
Msa0596610 MtrunA17_Chr2g0321741 44.828 87 46 1 27 113 12 96 2.98e-19 87.0
Msa0596610 MtrunA17_Chr8g0345731 40.909 110 61 3 7 113 24 132 1.93e-18 81.3
Msa0596610 MtrunA17_Chr8g0345701 38.462 130 62 5 1 113 1 129 4.24e-18 80.5
Msa0596610 MtrunA17_Chr4g0050701 38.318 107 53 3 7 111 11 106 5.92e-17 77.8
Msa0596610 MtrunA17_Chr7g0266701 28.000 225 100 8 7 171 4 226 6.90e-17 79.0
Msa0596610 MtrunA17_Chr3g0113001 55.385 65 27 1 51 113 4 68 8.03e-17 79.3
Msa0596610 MtrunA17_Chr8g0384351 32.530 166 65 7 7 146 10 154 1.01e-14 71.6
Msa0596610 MtrunA17_Chr8g0345741 41.414 99 54 3 18 113 2 99 1.53e-13 67.4
Msa0596610 MtrunA17_Chr3g0113031 65.854 41 14 0 73 113 3 43 4.46e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0596610 AT1G12260.1 48.744 398 122 14 1 323 1 391 3.02e-105 313
Msa0596610 AT1G12260.2 47.814 366 120 12 1 323 1 338 2.43e-96 289
Msa0596610 AT1G62700.1 48.077 364 109 14 1 301 1 347 3.35e-95 288
Msa0596610 AT1G62700.2 48.077 364 109 14 1 301 1 347 3.35e-95 288
Msa0596610 AT4G36160.2 45.294 340 130 8 7 303 14 340 6.71e-82 253
Msa0596610 AT4G36160.1 45.294 340 130 8 7 303 10 336 7.17e-82 253
Msa0596610 AT4G36160.3 45.294 340 130 8 7 303 10 336 7.17e-82 253
Msa0596610 AT2G18060.3 47.164 335 107 13 7 298 9 316 9.53e-79 244
Msa0596610 AT2G18060.1 47.164 335 107 13 7 298 9 316 9.53e-79 244
Msa0596610 AT5G66300.1 44.828 319 98 7 7 285 12 292 2.44e-77 238
Msa0596610 AT5G62380.2 40.688 349 120 10 1 296 1 315 6.71e-75 234
Msa0596610 AT5G62380.1 40.688 349 120 10 1 296 1 315 6.71e-75 234
Msa0596610 AT2G46770.1 40.503 358 101 12 1 301 10 312 2.31e-67 215
Msa0596610 AT3G61910.1 38.806 335 102 7 1 289 5 282 1.79e-62 202
Msa0596610 AT1G71930.2 59.091 154 32 1 5 127 7 160 4.35e-61 198
Msa0596610 AT1G71930.1 59.091 154 32 1 5 127 7 160 4.35e-61 198
Msa0596610 AT1G32770.1 56.213 169 31 1 1 126 10 178 3.46e-58 191
Msa0596610 AT4G10350.1 36.825 315 147 7 7 288 9 304 8.32e-57 187
Msa0596610 AT1G79580.3 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0596610 AT1G79580.5 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0596610 AT1G79580.4 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0596610 AT1G79580.2 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0596610 AT1G79580.1 60.927 151 28 2 7 126 17 167 1.45e-56 187
Msa0596610 AT1G33280.1 57.333 150 34 1 7 126 8 157 6.51e-56 184
Msa0596610 AT3G17730.1 46.199 171 75 5 7 171 6 165 3.28e-41 144
Msa0596610 AT3G18400.1 46.053 152 47 2 6 125 4 152 6.34e-40 142
Msa0596610 AT5G39610.1 44.910 167 54 3 7 138 20 183 2.10e-39 140
Msa0596610 AT1G54330.1 44.586 157 56 3 7 133 6 161 2.99e-39 140
Msa0596610 AT5G17260.1 45.806 155 49 3 7 127 6 159 4.39e-39 144
Msa0596610 AT3G03200.1 46.452 155 48 3 7 127 6 159 6.04e-39 144
Msa0596610 AT3G04060.1 32.886 298 119 10 7 263 20 277 1.18e-38 140
Msa0596610 AT2G24430.2 45.860 157 48 3 7 129 16 169 1.26e-38 139
Msa0596610 AT2G24430.1 45.860 157 48 3 7 129 16 169 1.26e-38 139
Msa0596610 AT1G65910.1 57.009 107 45 1 7 113 6 111 1.93e-38 144
Msa0596610 AT5G61430.1 45.455 154 46 3 7 125 16 166 3.35e-38 139
Msa0596610 AT3G29035.1 57.009 107 43 1 7 113 24 127 4.80e-38 137
Msa0596610 AT5G07680.2 57.009 107 43 1 7 113 3 106 1.87e-37 136
Msa0596610 AT3G12977.1 38.498 213 75 6 5 165 16 224 2.09e-37 135
Msa0596610 AT5G07680.1 57.009 107 43 1 7 113 17 120 2.71e-37 136
Msa0596610 AT1G76420.1 44.444 153 48 3 7 125 22 171 6.66e-37 135
Msa0596610 AT5G46590.2 54.545 110 48 2 5 113 4 112 9.89e-37 133
Msa0596610 AT5G46590.1 54.545 110 48 2 5 113 4 112 1.24e-36 133
Msa0596610 AT3G04070.2 54.386 114 44 2 5 113 8 118 2.55e-36 134
Msa0596610 AT1G69490.1 39.634 164 59 2 5 131 7 167 3.74e-36 131
Msa0596610 AT3G04070.1 54.386 114 44 2 5 113 8 118 3.81e-36 134
Msa0596610 AT3G15170.1 56.757 111 43 2 5 113 18 125 5.78e-36 132
Msa0596610 AT3G10490.1 55.046 109 48 1 5 113 25 132 1.20e-35 129
Msa0596610 AT4G35580.1 55.856 111 48 1 3 113 5 114 1.26e-35 135
Msa0596610 AT3G10480.2 56.075 107 46 1 7 113 27 132 1.46e-35 134
Msa0596610 AT3G10480.1 56.075 107 46 1 7 113 27 132 1.48e-35 134
Msa0596610 AT4G35580.3 55.856 111 48 1 3 113 5 114 1.51e-35 135
Msa0596610 AT4G35580.2 55.856 111 48 1 3 113 5 114 1.54e-35 135
Msa0596610 AT2G43000.1 40.000 155 55 3 9 129 20 170 1.79e-35 130
Msa0596610 AT4G17980.1 54.955 111 47 2 5 113 4 113 3.01e-35 129
Msa0596610 AT4G17980.2 54.955 111 47 2 5 113 4 113 5.76e-35 129
Msa0596610 AT1G26870.1 37.427 171 60 3 9 135 18 185 6.77e-35 131
Msa0596610 AT1G61110.1 52.294 109 48 2 6 113 15 120 7.99e-35 129
Msa0596610 AT5G18270.1 42.169 166 56 5 7 135 21 183 1.00e-34 129
Msa0596610 AT3G10490.2 57.143 105 44 1 9 113 29 132 1.04e-34 132
Msa0596610 AT5G18270.2 42.169 166 56 5 7 135 21 183 1.09e-34 129
Msa0596610 AT5G53950.1 54.545 110 45 2 6 113 16 122 1.51e-34 130
Msa0596610 AT1G33060.2 55.140 107 47 1 7 113 24 129 1.74e-34 133
Msa0596610 AT1G33060.1 55.140 107 47 1 7 113 24 129 1.96e-34 133
Msa0596610 AT5G63790.2 52.778 108 47 2 6 113 59 162 2.59e-34 128
Msa0596610 AT5G63790.1 52.778 108 47 2 6 113 49 152 3.09e-34 127
Msa0596610 AT1G56010.2 40.244 164 58 3 5 131 17 177 4.54e-34 127
Msa0596610 AT5G08790.1 51.327 113 51 2 1 113 1 109 1.28e-33 125
Msa0596610 AT5G04410.1 35.079 191 85 4 2 155 4 192 1.48e-33 130
Msa0596610 AT5G39820.1 50.459 109 49 2 7 113 20 125 2.18e-33 126
Msa0596610 AT3G10500.1 33.182 220 94 5 2 170 4 221 1.15e-32 127
Msa0596610 AT5G24590.2 52.679 112 51 2 3 113 9 119 1.55e-32 125
Msa0596610 AT1G01720.1 48.673 113 54 2 1 113 1 109 1.62e-32 122
Msa0596610 AT4G27410.2 39.474 152 56 2 7 125 14 162 1.63e-32 122
Msa0596610 AT3G49530.2 52.778 108 49 2 7 113 13 119 2.39e-32 124
Msa0596610 AT3G49530.1 52.778 108 49 2 7 113 13 119 3.56e-32 125
Msa0596610 AT1G77450.1 50.926 108 49 2 6 113 9 112 5.30e-32 120
Msa0596610 AT2G33480.2 40.789 152 53 2 6 126 14 159 2.90e-31 118
Msa0596610 AT2G33480.1 50.926 108 47 1 6 113 14 115 3.07e-31 118
Msa0596610 AT2G02450.2 48.148 108 52 2 7 113 51 155 4.37e-31 121
Msa0596610 AT2G02450.1 48.148 108 52 2 7 113 51 155 4.51e-31 120
Msa0596610 AT2G27300.1 46.789 109 56 1 5 113 12 118 6.73e-31 119
Msa0596610 AT1G32510.1 47.414 116 53 2 5 113 4 118 6.95e-31 118
Msa0596610 AT4G27410.3 46.281 121 51 2 7 113 14 134 1.12e-30 118
Msa0596610 AT3G10480.3 44.286 140 44 3 7 113 27 165 2.04e-30 120
Msa0596610 AT3G15500.1 36.538 156 63 2 7 129 14 166 3.66e-30 117
Msa0596610 AT4G28530.1 37.870 169 53 5 5 125 8 172 4.06e-30 117
Msa0596610 AT4G10350.2 31.852 270 132 7 52 288 7 257 4.36e-30 116
Msa0596610 AT4G28530.2 42.029 138 53 4 5 125 8 135 5.37e-30 116
Msa0596610 AT1G52890.1 36.538 156 63 2 7 129 14 166 1.61e-29 115
Msa0596610 AT1G52880.1 55.435 92 38 1 11 102 21 109 3.22e-29 114
Msa0596610 AT1G34180.3 45.714 105 56 1 9 113 18 121 6.18e-29 115
Msa0596610 AT1G34180.1 45.714 105 56 1 9 113 18 121 7.54e-29 116
Msa0596610 AT5G64060.1 28.470 281 143 7 5 247 4 264 1.08e-28 114
Msa0596610 AT3G15510.1 48.571 105 49 2 11 113 21 122 1.45e-28 113
Msa0596610 AT5G09330.5 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0596610 AT5G09330.2 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0596610 AT5G09330.4 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0596610 AT5G09330.3 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0596610 AT5G09330.1 32.474 194 96 3 5 165 4 195 4.93e-28 114
Msa0596610 AT5G22290.1 44.037 109 59 1 5 113 19 125 6.77e-28 111
Msa0596610 AT3G44290.2 42.857 105 58 1 9 113 16 118 8.27e-28 108
Msa0596610 AT2G17040.1 45.370 108 53 2 9 113 8 112 9.05e-28 109
Msa0596610 AT5G13180.1 37.107 159 60 4 2 126 9 161 9.91e-28 108
Msa0596610 AT1G34190.1 46.667 105 55 1 9 113 18 121 1.35e-27 113
Msa0596610 AT3G44290.1 42.857 105 58 1 9 113 16 118 3.93e-27 109
Msa0596610 AT5G04395.1 45.082 122 54 3 5 113 26 147 5.60e-27 105
Msa0596610 AT1G34180.4 40.517 116 58 1 9 113 18 133 3.88e-26 108
Msa0596610 AT1G34180.2 40.517 116 58 1 9 113 18 133 3.95e-26 108
Msa0596610 AT3G12910.1 47.748 111 47 4 9 113 23 128 7.66e-26 105
Msa0596610 AT1G32870.1 45.794 107 55 2 7 113 10 113 4.21e-22 97.1
Msa0596610 AT1G32870.2 45.794 107 55 2 7 113 10 113 4.21e-22 97.1
Msa0596610 AT1G32870.3 45.794 107 55 2 7 113 46 149 4.53e-22 97.1
Msa0596610 AT3G12977.2 34.545 165 54 4 52 165 10 171 2.22e-20 88.6
Msa0596610 AT2G18060.2 34.855 241 91 12 88 285 2 219 7.00e-20 88.2
Msa0596610 AT1G02230.1 44.628 121 59 5 8 126 4 118 8.52e-20 89.4
Msa0596610 AT5G64530.2 41.284 109 51 3 7 113 3 100 1.84e-19 84.3
Msa0596610 AT5G22380.1 41.441 111 55 5 10 113 8 115 4.91e-19 85.1
Msa0596610 AT1G02250.1 45.378 119 57 5 8 124 4 116 5.59e-19 87.0
Msa0596610 AT5G64530.1 41.284 109 51 3 7 113 3 100 6.65e-19 83.6
Msa0596610 AT5G61430.2 43.878 98 20 2 63 125 6 103 1.77e-18 84.3
Msa0596610 AT1G56010.1 39.806 103 26 1 65 131 8 110 1.97e-17 80.9
Msa0596610 AT3G44350.1 38.182 110 59 5 10 113 8 114 5.00e-16 74.7
Msa0596610 AT3G44350.2 38.182 110 59 5 10 113 8 114 6.70e-16 76.3
Msa0596610 AT3G04420.2 40.367 109 60 3 8 113 4 110 1.70e-15 75.9
Msa0596610 AT4G01520.1 37.838 111 63 4 8 113 5 114 2.43e-15 75.9
Msa0596610 AT3G04420.1 40.367 109 60 3 8 113 4 110 3.32e-15 75.9
Msa0596610 AT4G01540.1 37.615 109 62 4 10 113 7 114 1.68e-14 74.3
Msa0596610 AT4G01540.3 37.615 109 62 4 10 113 7 114 1.74e-14 74.3
Msa0596610 AT4G01540.4 37.615 109 62 4 10 113 7 114 1.86e-14 73.9
Msa0596610 AT4G01540.2 37.615 109 62 4 10 113 7 114 2.03e-14 73.9
Msa0596610 AT4G01550.1 32.022 178 100 6 10 173 7 177 2.43e-14 73.9
Msa0596610 AT1G02220.1 40.367 109 62 3 7 113 3 110 4.58e-14 72.8
Msa0596610 AT2G33480.3 51.786 56 27 0 58 113 3 58 8.18e-13 67.0

Find 65 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATACTTCTTATCTTTA+TGG 0.078281 4_2:+27188677 None:intergenic
CAATATTGAATTCAGAAATT+TGG 0.171673 4_2:-27186987 Msa0596610:CDS
GAGCAACTGCAGCTGGATTT+TGG 0.212597 4_2:-27188636 Msa0596610:CDS
TGTTGTAACCATTGGATTAG+AGG 0.295684 4_2:+27187249 None:intergenic
TAATTGTTGGTATATCAAAT+TGG 0.305571 4_2:+27187393 None:intergenic
TAGTCAAAGGAAAAGGTCTT+TGG 0.307210 4_2:+27190523 None:intergenic
AATTGTCACGAGACATGTTT+TGG 0.317098 4_2:+27187329 None:intergenic
AATAATAACAACATCTTTCA+TGG 0.344913 4_2:-27187010 Msa0596610:CDS
TTGACTAATTAATTATATCT+TGG 0.350029 4_2:-27190507 Msa0596610:intron
GAAGAAGATTGAACAAGCTT+TGG 0.352286 4_2:+27187280 None:intergenic
AGTTTCATTCATGCAAGATA+TGG 0.353877 4_2:-27187440 Msa0596610:CDS
TTTATAGAGATTTGCAGAAT+AGG 0.359693 4_2:-27188733 Msa0596610:intron
TATAGTAAGCATAATTTGAT+AGG 0.379805 4_2:-27188586 Msa0596610:CDS
AAAGATAAGAAGTATCCAAC+TGG 0.410251 4_2:-27188673 Msa0596610:CDS
CTATTTGTTCTTGTCCCAGT+TGG 0.412869 4_2:+27188658 None:intergenic
CAAAACTTTCATGCTATGTA+TGG 0.416700 4_2:-27187142 Msa0596610:CDS
AGTCAAAGGAAAAGGTCTTT+GGG 0.417990 4_2:+27190524 None:intergenic
CAAACACAAGTTGGATATGA+TGG 0.442162 4_2:-27186925 None:intergenic
TCAAATTGGATTGAGAGGTT+TGG 0.444509 4_2:+27187407 None:intergenic
GTGTCATATGTTGTAACCAT+TGG 0.449797 4_2:+27187241 None:intergenic
ACAAATAGAGCAACTGCAGC+TGG 0.455592 4_2:-27188643 Msa0596610:CDS
GGAAGAAAATTGATTCAAGA+AGG 0.463332 4_2:-27189204 Msa0596610:CDS
AAATGAAAGCAAAGGCAAGC+TGG 0.470082 4_2:+27190666 None:intergenic
CCTCTACATCAAACACAAGT+TGG 0.475978 4_2:-27186934 Msa0596610:CDS
TTCCAATGTTGAACAAGGTA+TGG 0.483739 4_2:-27187113 Msa0596610:CDS
CCAACTTGTGTTTGATGTAG+AGG 0.486977 4_2:+27186934 None:intergenic
TTGGATTGAGAGGTTTGGTT+TGG 0.487041 4_2:+27187412 None:intergenic
CCTTTACAAAATTGAACCAT+GGG 0.489236 4_2:-27189152 Msa0596610:CDS
AACCATACCTTGTTCAACAT+TGG 0.489325 4_2:+27187111 None:intergenic
GTGAGCATGACTCTCCTTGT+TGG 0.496787 4_2:-27187474 Msa0596610:CDS
ATTGAACCATGGGATCTTGA+AGG 0.503378 4_2:-27189142 Msa0596610:intron
TTGATGATCAAGTTACTGAT+TGG 0.505048 4_2:-27187090 Msa0596610:CDS
GTTTGGAGCAGGAGGAAGGA+TGG 0.509198 4_2:-27187549 Msa0596610:intron
AACTTGTGGATTACTACCTT+AGG 0.520831 4_2:-27189225 Msa0596610:CDS
GATAGGAATGAGGAAAACAT+TGG 0.522127 4_2:-27188569 Msa0596610:intron
GACGGTTTGGAGCAGGAGGA+AGG 0.522417 4_2:-27187553 Msa0596610:intron
AATTAATTAGTCAAAGGAAA+AGG 0.523043 4_2:+27190516 None:intergenic
AAATTTGGAGAAACAAGAAA+TGG 0.525812 4_2:-27186972 Msa0596610:CDS
AAGAATATGATGGTAATTGT+TGG 0.533908 4_2:+27187380 None:intergenic
TGGGATGGAACCTAAACCCC+GGG 0.538292 4_2:+27189257 None:intergenic
TCCCACAGATGAGGAACTTG+TGG 0.541887 4_2:-27189239 Msa0596610:CDS
TTTGGAGCAGGAGGAAGGAT+GGG 0.544311 4_2:-27187548 Msa0596610:intron
AATGAAAGCAAAGGCAAGCT+GGG 0.545140 4_2:+27190667 None:intergenic
GTATATCAAATTGGATTGAG+AGG 0.548657 4_2:+27187402 None:intergenic
ATCCACAAGTTCCTCATCTG+TGG 0.551212 4_2:+27189237 None:intergenic
ACCTTTACAAAATTGAACCA+TGG 0.552414 4_2:-27189153 Msa0596610:CDS
ACAAGAAGAAATGAAAGCAA+AGG 0.555525 4_2:+27190658 None:intergenic
AGCATAATTTGATAGGAATG+AGG 0.556802 4_2:-27188579 Msa0596610:CDS
GAAGAACAAGTTATACAAAG+TGG 0.557940 4_2:-27187172 Msa0596610:CDS
CTTATACCTTCAAGATCCCA+TGG 0.563913 4_2:+27189136 None:intergenic
AAACATAACCTCTAATCCAA+TGG 0.567146 4_2:-27187257 Msa0596610:CDS
TCTTGTAAGAGCATTGATGA+AGG 0.569946 4_2:+27187193 None:intergenic
AGCTTTGGACTCTCTAGAAG+TGG 0.581594 4_2:+27187295 None:intergenic
CAAGTTCCTCATCTGTGGGA+TGG 0.581878 4_2:+27189242 None:intergenic
TCCACAAGTTCCTCATCTGT+GGG 0.587470 4_2:+27189238 None:intergenic
AGATATAATTAATTAGTCAA+AGG 0.591128 4_2:+27190510 None:intergenic
TAGGTTCCATCCCACAGATG+AGG 0.602397 4_2:-27189248 Msa0596610:CDS
AACAATTCCAATGTTGAACA+AGG 0.643045 4_2:-27187118 Msa0596610:CDS
GTGGGATGGAACCTAAACCC+CGG 0.647443 4_2:+27189256 None:intergenic
GAAAGCAACAGGAAGAGACA+AGG 0.657167 4_2:-27188614 Msa0596610:CDS
GAACCGAAGAACAAAATGAG+TGG 0.672469 4_2:-27188711 Msa0596610:CDS
AAGATAAGAAGTATCCAACT+GGG 0.681306 4_2:-27188672 Msa0596610:CDS
ACTTGATCTTCATACCAACA+AGG 0.708859 4_2:+27187460 None:intergenic
GGATGGAACCTAAACCCCGG+GGG 0.731147 4_2:+27189259 None:intergenic
GGGATGGAACCTAAACCCCG+GGG 0.785968 4_2:+27189258 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTCTTTTATGAAAATT+AGG - chr4_2:27188043-27188062 Msa0596610:intron 10.0%
!! TCAAAAAAGAAAAATAAAAA+AGG + chr4_2:27188872-27188891 None:intergenic 10.0%
!! AGATATAATTAATTAGTCAA+AGG + chr4_2:27187119-27187138 None:intergenic 15.0%
!! TTGACTAATTAATTATATCT+TGG - chr4_2:27187119-27187138 Msa0596610:CDS 15.0%
!!! TTTATACATCAACTAATTTT+TGG - chr4_2:27187243-27187262 Msa0596610:CDS 15.0%
!!! ATTTTCAAAATATTCTATGT+CGG - chr4_2:27187507-27187526 Msa0596610:CDS 15.0%
!! CTAAGAAAAATTTAAACTTA+GGG + chr4_2:27187680-27187699 None:intergenic 15.0%
!!! AAAATATTTTATAGAGAAAG+AGG - chr4_2:27188128-27188147 Msa0596610:intron 15.0%
!! TATTTGATATATGCTTAATT+TGG - chr4_2:27188234-27188253 Msa0596610:intron 15.0%
!! AATTAGAAAAGTTAATTGAT+AGG + chr4_2:27188287-27188306 None:intergenic 15.0%
!! TCTAAATATATGTTTATAAG+TGG + chr4_2:27189601-27189620 None:intergenic 15.0%
!! AATTAATTAGTCAAAGGAAA+AGG + chr4_2:27187113-27187132 None:intergenic 20.0%
!! CTAATTAATTATATCTTGGT+TGG - chr4_2:27187123-27187142 Msa0596610:CDS 20.0%
!! TAATTTAGTGAAAGAATTAG+AGG + chr4_2:27187369-27187388 None:intergenic 20.0%
!! CCTAAGAAAAATTTAAACTT+AGG + chr4_2:27187681-27187700 None:intergenic 20.0%
!!! CCTAAGTTTAAATTTTTCTT+AGG - chr4_2:27187678-27187697 Msa0596610:intron 20.0%
!! GAATAATGTTAGACAAAATA+AGG - chr4_2:27187700-27187719 Msa0596610:intron 20.0%
!! TGATATATGACATATATACA+TGG + chr4_2:27187903-27187922 None:intergenic 20.0%
!! CATATATCATGAAACAATAT+TGG - chr4_2:27187914-27187933 Msa0596610:intron 20.0%
!! TTTAAAGAATGTCAAATTGT+GGG + chr4_2:27187972-27187991 None:intergenic 20.0%
!! ATTTAAAGAATGTCAAATTG+TGG + chr4_2:27187973-27187992 None:intergenic 20.0%
!! ACATTCTTTAAATTCTTGTA+AGG - chr4_2:27187980-27187999 Msa0596610:intron 20.0%
!! CATTCTTTAAATTCTTGTAA+GGG - chr4_2:27187981-27188000 Msa0596610:intron 20.0%
!!! TGCTTGTTGAATAAAAAAAA+AGG + chr4_2:27188105-27188124 None:intergenic 20.0%
!! TCAATGAATAAAGTGATATT+TGG + chr4_2:27188260-27188279 None:intergenic 20.0%
!!! AATTTGATATATGTGTTTTC+AGG - chr4_2:27188303-27188322 Msa0596610:intron 20.0%
!! TTTGGATTAATATCAAAGTT+TGG + chr4_2:27188566-27188585 None:intergenic 20.0%
!!! AAATAGTGAACTTTTTTTGT+GGG + chr4_2:27188706-27188725 None:intergenic 20.0%
!!! TAAATAGTGAACTTTTTTTG+TGG + chr4_2:27188707-27188726 None:intergenic 20.0%
!! AAAATAATTTGATACTGAAG+CGG + chr4_2:27188734-27188753 None:intergenic 20.0%
!!! TTATTTTTCAGTTTCTTAGT+AGG - chr4_2:27188747-27188766 Msa0596610:intron 20.0%
!! TATAGTAAGCATAATTTGAT+AGG - chr4_2:27189040-27189059 Msa0596610:intron 20.0%
!!! AAAACATTGGTGTTTTATAA+AGG - chr4_2:27189070-27189089 Msa0596610:intron 20.0%
!! TCTCTAGATTTATTTAATGT+TGG - chr4_2:27189194-27189213 Msa0596610:CDS 20.0%
!!! GTAATTCTCATTTTACAAAT+CGG + chr4_2:27189575-27189594 None:intergenic 20.0%
!!! ATGTTATAGATTAGTTTGTA+CGG - chr4_2:27189878-27189897 Msa0596610:intron 20.0%
!!! TTTGTTTTTGTTATTTTCGA+CGG - chr4_2:27190055-27190074 Msa0596610:intron 20.0%
!! TAATTGTTGGTATATCAAAT+TGG + chr4_2:27190236-27190255 None:intergenic 20.0%
!! AATAATAACAACATCTTTCA+TGG - chr4_2:27190616-27190635 Msa0596610:five_prime_UTR 20.0%
!! CAATATTGAATTCAGAAATT+TGG - chr4_2:27190639-27190658 Msa0596610:five_prime_UTR 20.0%
!! TAACATTGTTGTGTTTTTCT+TGG - chr4_2:27187032-27187051 Msa0596610:CDS 25.0%
!! AACATTGTTGTGTTTTTCTT+GGG - chr4_2:27187033-27187052 Msa0596610:CDS 25.0%
!!! ATACATCAACTAATTTTTGG+AGG - chr4_2:27187246-27187265 Msa0596610:CDS 25.0%
! AAAGAAATTTCAGCACAAAA+AGG - chr4_2:27187578-27187597 Msa0596610:intron 25.0%
!!! TTTTTTATCCTTTAGAAGCA+TGG - chr4_2:27187736-27187755 Msa0596610:intron 25.0%
! AGCATATATCAAATAGACTT+TGG + chr4_2:27188229-27188248 None:intergenic 25.0%
!!! TTTTCAGGATTATAAAAGAG+AGG - chr4_2:27188318-27188337 Msa0596610:intron 25.0%
!! TTGAATCAATTTTCTTCCTA+AGG + chr4_2:27188420-27188439 None:intergenic 25.0%
! TTTATAGAGATTTGCAGAAT+AGG - chr4_2:27188893-27188912 Msa0596610:intron 25.0%
! TTGGATACTTCTTATCTTTA+TGG + chr4_2:27188952-27188971 None:intergenic 25.0%
!!! TTTACAAATCGGTTTTGTAA+TGG + chr4_2:27189564-27189583 None:intergenic 25.0%
! GCAATAGATAAGTCTAATAT+TGG + chr4_2:27189635-27189654 None:intergenic 25.0%
! AGTTGAAATCGTAATTTCTA+TGG - chr4_2:27189695-27189714 Msa0596610:intron 25.0%
! CATATACACAACAAAATATG+AGG - chr4_2:27189722-27189741 Msa0596610:intron 25.0%
!! AACAATAACAATTAGGGTTT+TGG + chr4_2:27189798-27189817 None:intergenic 25.0%
! ATCGACAACAATAACAATTA+GGG + chr4_2:27189804-27189823 None:intergenic 25.0%
!!! TGTTATAGATTAGTTTGTAC+GGG - chr4_2:27189879-27189898 Msa0596610:intron 25.0%
!!! TAACAAATGTTAGTCTTCTA+AGG + chr4_2:27189994-27190013 None:intergenic 25.0%
! AAGAATATGATGGTAATTGT+TGG + chr4_2:27190249-27190268 None:intergenic 25.0%
!!! TCTTTTTTGCAAGAATATGA+TGG + chr4_2:27190259-27190278 None:intergenic 25.0%
! AAATTTGGAGAAACAAGAAA+TGG - chr4_2:27190654-27190673 Msa0596610:five_prime_UTR 25.0%
ACAAGAAGAAATGAAAGCAA+AGG + chr4_2:27186971-27186990 None:intergenic 30.0%
AAACAGACAAAAAAGTTGAG+AGG - chr4_2:27187071-27187090 Msa0596610:CDS 30.0%
AACAGACAAAAAAGTTGAGA+GGG - chr4_2:27187072-27187091 Msa0596610:CDS 30.0%
! TTTTTGCAAGATCAACAATG+TGG + chr4_2:27187561-27187580 None:intergenic 30.0%
!! CTTAGGGTTTTTAGAAAATG+TGG + chr4_2:27187664-27187683 None:intergenic 30.0%
ATTTATCACCATGCTTCTAA+AGG + chr4_2:27187747-27187766 None:intergenic 30.0%
AAGCATGGTGATAAATAAGT+GGG - chr4_2:27187751-27187770 Msa0596610:intron 30.0%
! GGAAGAAAATTGATTCAAGA+AGG - chr4_2:27188422-27188441 Msa0596610:intron 30.0%
ACCTTTACAAAATTGAACCA+TGG - chr4_2:27188473-27188492 Msa0596610:intron 30.0%
CCTTTACAAAATTGAACCAT+GGG - chr4_2:27188474-27188493 Msa0596610:intron 30.0%
! TATCAAAGTTTGGAGCATAA+TGG + chr4_2:27188556-27188575 None:intergenic 30.0%
GATTGTATTTATGTGCAGTT+TGG + chr4_2:27188584-27188603 None:intergenic 30.0%
GACATTACGTAATATATGCA+GGG - chr4_2:27188640-27188659 Msa0596610:CDS 30.0%
ACATTACGTAATATATGCAG+GGG - chr4_2:27188641-27188660 Msa0596610:CDS 30.0%
! ATACCACTCATTTTGTTCTT+CGG + chr4_2:27188921-27188940 None:intergenic 30.0%
!! AAAGATAAGAAGTATCCAAC+TGG - chr4_2:27188953-27188972 Msa0596610:intron 30.0%
!! AAGATAAGAAGTATCCAACT+GGG - chr4_2:27188954-27188973 Msa0596610:intron 30.0%
AGCATAATTTGATAGGAATG+AGG - chr4_2:27189047-27189066 Msa0596610:intron 30.0%
ATTGGATAATGCATGAGTAT+AGG - chr4_2:27189119-27189138 Msa0596610:intron 30.0%
GTGTTTATCCAACATTCATT+GGG + chr4_2:27189504-27189523 None:intergenic 30.0%
TGTGTTTATCCAACATTCAT+TGG + chr4_2:27189505-27189524 None:intergenic 30.0%
ACAAATATCATCTTCGTGTT+TGG - chr4_2:27189522-27189541 Msa0596610:intron 30.0%
GATCGACAACAATAACAATT+AGG + chr4_2:27189805-27189824 None:intergenic 30.0%
GATAAAGTTGTGTTAACTCT+TGG - chr4_2:27189836-27189855 Msa0596610:intron 30.0%
!! AGACATTGAGAGTATTAAGA+AGG + chr4_2:27189949-27189968 None:intergenic 30.0%
!!! TTTTGTTATTTTCGACGGTT+TGG - chr4_2:27190060-27190079 Msa0596610:intron 30.0%
AGTTTCATTCATGCAAGATA+TGG - chr4_2:27190186-27190205 Msa0596610:intron 30.0%
GTATATCAAATTGGATTGAG+AGG + chr4_2:27190227-27190246 None:intergenic 30.0%
AAACATAACCTCTAATCCAA+TGG - chr4_2:27190369-27190388 Msa0596610:five_prime_UTR 30.0%
GAAGAACAAGTTATACAAAG+TGG - chr4_2:27190454-27190473 Msa0596610:intron 30.0%
CAAAACTTTCATGCTATGTA+TGG - chr4_2:27190484-27190503 Msa0596610:intron 30.0%
AACAATTCCAATGTTGAACA+AGG - chr4_2:27190508-27190527 Msa0596610:five_prime_UTR 30.0%
! TTGATGATCAAGTTACTGAT+TGG - chr4_2:27190536-27190555 Msa0596610:five_prime_UTR 30.0%
AGTCAAAGGAAAAGGTCTTT+GGG + chr4_2:27187105-27187124 None:intergenic 35.0%
TAGTCAAAGGAAAAGGTCTT+TGG + chr4_2:27187106-27187125 None:intergenic 35.0%
! ACTTGACTCTTGACACTTTT+TGG - chr4_2:27187279-27187298 Msa0596610:CDS 35.0%
TCAGCACAAAAAGGAAAGAT+AGG - chr4_2:27187587-27187606 Msa0596610:intron 35.0%
!! AGGGTTTTTAGAAAATGTGG+AGG + chr4_2:27187661-27187680 None:intergenic 35.0%
GAAGCATGGTGATAAATAAG+TGG - chr4_2:27187750-27187769 Msa0596610:intron 35.0%
GAACATGATGATTATGCATG+TGG + chr4_2:27187869-27187888 None:intergenic 35.0%
CAAATTGTGGGATCAAAATG+TGG + chr4_2:27187960-27187979 None:intergenic 35.0%
! AACTTGTGGATTACTACCTT+AGG - chr4_2:27188401-27188420 Msa0596610:intron 35.0%
! CCCATGGTTCAATTTTGTAA+AGG + chr4_2:27188477-27188496 None:intergenic 35.0%
GGACATTACGTAATATATGC+AGG - chr4_2:27188639-27188658 Msa0596610:CDS 35.0%
TGTGGGACTACAATATAAGT+GGG + chr4_2:27188689-27188708 None:intergenic 35.0%
TTGTGGGACTACAATATAAG+TGG + chr4_2:27188690-27188709 None:intergenic 35.0%
GATAGGAATGAGGAAAACAT+TGG - chr4_2:27189057-27189076 Msa0596610:intron 35.0%
! CAATCTGACTTTTGTCCATT+GGG + chr4_2:27189103-27189122 None:intergenic 35.0%
AGGCTTGAAACAGATGAAAA+TGG - chr4_2:27189139-27189158 Msa0596610:intron 35.0%
! AAGAGTTTCGCCAAAAGTAT+CGG - chr4_2:27189281-27189300 Msa0596610:CDS 35.0%
! TGGTTTCAAACCGATACTTT+TGG + chr4_2:27189294-27189313 None:intergenic 35.0%
! CTTACAAGTTCACCTTTTAG+GGG + chr4_2:27189336-27189355 None:intergenic 35.0%
! CCTTACAAGTTCACCTTTTA+GGG + chr4_2:27189337-27189356 None:intergenic 35.0%
! ACCTTACAAGTTCACCTTTT+AGG + chr4_2:27189338-27189357 None:intergenic 35.0%
ATGAGGCAAAATAAACCTAG+AGG - chr4_2:27189739-27189758 Msa0596610:intron 35.0%
ACTTGATCTTCATACCAACA+AGG + chr4_2:27190169-27190188 None:intergenic 35.0%
TCAAATTGGATTGAGAGGTT+TGG + chr4_2:27190222-27190241 None:intergenic 35.0%
! AATTGTCACGAGACATGTTT+TGG + chr4_2:27190300-27190319 None:intergenic 35.0%
GAAGAAGATTGAACAAGCTT+TGG + chr4_2:27190349-27190368 None:intergenic 35.0%
! TGTTGTAACCATTGGATTAG+AGG + chr4_2:27190380-27190399 None:intergenic 35.0%
GTGTCATATGTTGTAACCAT+TGG + chr4_2:27190388-27190407 None:intergenic 35.0%
!! TCTTGTAAGAGCATTGATGA+AGG + chr4_2:27190436-27190455 None:intergenic 35.0%
TTCCAATGTTGAACAAGGTA+TGG - chr4_2:27190513-27190532 Msa0596610:five_prime_UTR 35.0%
AACCATACCTTGTTCAACAT+TGG + chr4_2:27190518-27190537 None:intergenic 35.0%
AATGAAAGCAAAGGCAAGCT+GGG + chr4_2:27186962-27186981 None:intergenic 40.0%
AAATGAAAGCAAAGGCAAGC+TGG + chr4_2:27186963-27186982 None:intergenic 40.0%
!! TGACTCTTGACACTTTTTGG+AGG - chr4_2:27187282-27187301 Msa0596610:CDS 40.0%
!!! TACTTTTTCTCATGTTCCCC+CGG - chr4_2:27188351-27188370 Msa0596610:intron 40.0%
ATTGAACCATGGGATCTTGA+AGG - chr4_2:27188484-27188503 Msa0596610:intron 40.0%
CTTATACCTTCAAGATCCCA+TGG + chr4_2:27188493-27188512 None:intergenic 40.0%
GAGCATAATGGCAACAACAA+CGG + chr4_2:27188544-27188563 None:intergenic 40.0%
TCTCATGTGTGTAGCTCATT+TGG - chr4_2:27188613-27188632 Msa0596610:CDS 40.0%
!! CAATATAAGTGGGTTGTCCA+GGG + chr4_2:27188679-27188698 None:intergenic 40.0%
!! ACAATATAAGTGGGTTGTCC+AGG + chr4_2:27188680-27188699 None:intergenic 40.0%
GAACCGAAGAACAAAATGAG+TGG - chr4_2:27188915-27188934 Msa0596610:intron 40.0%
CTATTTGTTCTTGTCCCAGT+TGG + chr4_2:27188971-27188990 None:intergenic 40.0%
! CCAATCTGACTTTTGTCCAT+TGG + chr4_2:27189104-27189123 None:intergenic 40.0%
CCAATGGACAAAAGTCAGAT+TGG - chr4_2:27189101-27189120 Msa0596610:intron 40.0%
!!! AGTTTTGAATCACTTGCCTG+TGG + chr4_2:27189169-27189188 None:intergenic 40.0%
!! AAGTTCACCTTTTAGGGGTT+GGG + chr4_2:27189331-27189350 None:intergenic 40.0%
CCCTAAAAGGTGAACTTGTA+AGG - chr4_2:27189334-27189353 Msa0596610:intron 40.0%
AAGAGTGACCCAATGAATGT+TGG - chr4_2:27189493-27189512 Msa0596610:intron 40.0%
GGAAAAAGTTCCAAGACTCT+TGG + chr4_2:27189777-27189796 None:intergenic 40.0%
TAAGGCGTAGTACAATGGTA+TGG + chr4_2:27189976-27189995 None:intergenic 40.0%
!! TCTTCTAAGGCGTAGTACAA+TGG + chr4_2:27189981-27190000 None:intergenic 40.0%
! TTGGATTGAGAGGTTTGGTT+TGG + chr4_2:27190217-27190236 None:intergenic 40.0%
!!! CACGAGACATGTTTTGGAAT+GGG + chr4_2:27190294-27190313 None:intergenic 40.0%
!!! TCACGAGACATGTTTTGGAA+TGG + chr4_2:27190295-27190314 None:intergenic 40.0%
!!! GATGTAGAGGCATTTTCATG+AGG + chr4_2:27190682-27190701 None:intergenic 40.0%
CCAACTTGTGTTTGATGTAG+AGG + chr4_2:27190695-27190714 None:intergenic 40.0%
CCTCTACATCAAACACAAGT+TGG - chr4_2:27190692-27190711 Msa0596610:five_prime_UTR 40.0%
!! ACTTTTTCTCATGTTCCCCC+GGG - chr4_2:27188352-27188371 Msa0596610:intron 45.0%
TCCACAAGTTCCTCATCTGT+GGG + chr4_2:27188391-27188410 None:intergenic 45.0%
ATCCACAAGTTCCTCATCTG+TGG + chr4_2:27188392-27188411 None:intergenic 45.0%
TGTGTGTAGCTCATTTGGCA+TGG - chr4_2:27188618-27188637 Msa0596610:CDS 45.0%
ACAAATAGAGCAACTGCAGC+TGG - chr4_2:27188983-27189002 Msa0596610:intron 45.0%
!! GCTGGATTTTGGAAAGCAAC+AGG - chr4_2:27189001-27189020 Msa0596610:intron 45.0%
GAAAGCAACAGGAAGAGACA+AGG - chr4_2:27189012-27189031 Msa0596610:intron 45.0%
TATAAAGGTCGAGCTCCCAA+TGG - chr4_2:27189085-27189104 Msa0596610:intron 45.0%
AGATGAAAATGGCACACCAC+AGG - chr4_2:27189150-27189169 Msa0596610:CDS 45.0%
!! CAAGTTCACCTTTTAGGGGT+TGG + chr4_2:27189332-27189351 None:intergenic 45.0%
CATTCCAAACGTCCAACCTT+AGG + chr4_2:27189440-27189459 None:intergenic 45.0%
!! GTTTGGAATGCGACTTGGTT+GGG - chr4_2:27189450-27189469 Msa0596610:intron 45.0%
!! TTGGGCTAGTACTGGTAACA+TGG - chr4_2:27189468-27189487 Msa0596610:intron 45.0%
!! TGGGCTAGTACTGGTAACAT+GGG - chr4_2:27189469-27189488 Msa0596610:intron 45.0%
AAGTTCCAAGACTCTTGGCT+TGG + chr4_2:27189772-27189791 None:intergenic 45.0%
!! ATTAGTTTGTACGGGTCGAG+AGG - chr4_2:27189887-27189906 Msa0596610:intron 45.0%
!! TATTTTCGACGGTTTGGAGC+AGG - chr4_2:27190066-27190085 Msa0596610:intron 45.0%
!! AGCTTTGGACTCTCTAGAAG+TGG + chr4_2:27190334-27190353 None:intergenic 45.0%
! CTTTTTCTCATGTTCCCCCG+GGG - chr4_2:27188353-27188372 Msa0596610:intron 50.0%
TAGGTTCCATCCCACAGATG+AGG - chr4_2:27188378-27188397 Msa0596610:intron 50.0%
CAAGTTCCTCATCTGTGGGA+TGG + chr4_2:27188387-27188406 None:intergenic 50.0%
TCCCACAGATGAGGAACTTG+TGG - chr4_2:27188387-27188406 Msa0596610:intron 50.0%
CGTAATATATGCAGGGGTCG+TGG - chr4_2:27188647-27188666 Msa0596610:CDS 50.0%
! GAGCAACTGCAGCTGGATTT+TGG - chr4_2:27188990-27189009 Msa0596610:intron 50.0%
! GTTGGGTTAGGCTCAACTCA+TGG + chr4_2:27189314-27189333 None:intergenic 50.0%
AGCCTAACCCAACCCCTAAA+AGG - chr4_2:27189321-27189340 Msa0596610:intron 50.0%
!! CACCTTTTAGGGGTTGGGTT+AGG + chr4_2:27189326-27189345 None:intergenic 50.0%
! TGGACGTTTGGAATGCGACT+TGG - chr4_2:27189445-27189464 Msa0596610:intron 50.0%
!! CGTTTGGAATGCGACTTGGT+TGG - chr4_2:27189449-27189468 Msa0596610:intron 50.0%
! TGGCTTGGACTACATCCTCT+AGG + chr4_2:27189757-27189776 None:intergenic 50.0%
GTAGTCCAAGCCAAGAGTCT+TGG - chr4_2:27189764-27189783 Msa0596610:intron 50.0%
! TTTGGAGCAGGAGGAAGGAT+GGG - chr4_2:27190078-27190097 Msa0596610:intron 50.0%
GTGAGCATGACTCTCCTTGT+TGG - chr4_2:27190152-27190171 Msa0596610:intron 50.0%
TGGGATGGAACCTAAACCCC+GGG + chr4_2:27188372-27188391 None:intergenic 55.0%
GTGGGATGGAACCTAAACCC+CGG + chr4_2:27188373-27188392 None:intergenic 55.0%
GCGTGAGTGGTGTGTGTTGA+AGG + chr4_2:27189418-27189437 None:intergenic 55.0%
ACACACACCACTCACGCCTA+AGG - chr4_2:27189421-27189440 Msa0596610:intron 55.0%
ACACCACTCACGCCTAAGGT+TGG - chr4_2:27189425-27189444 Msa0596610:intron 55.0%
CACGCCTAAGGTTGGACGTT+TGG - chr4_2:27189433-27189452 Msa0596610:intron 55.0%
!! CGACTTGGTTGGGCTAGTAC+TGG - chr4_2:27189460-27189479 Msa0596610:intron 55.0%
! TTTCGACGGTTTGGAGCAGG+AGG - chr4_2:27190069-27190088 Msa0596610:intron 55.0%
! GTTTGGAGCAGGAGGAAGGA+TGG - chr4_2:27190077-27190096 Msa0596610:intron 55.0%
CTCATGTTCCCCCGGGGTTT+AGG - chr4_2:27188359-27188378 Msa0596610:intron 60.0%
GGATGGAACCTAAACCCCGG+GGG + chr4_2:27188370-27188389 None:intergenic 60.0%
GGGATGGAACCTAAACCCCG+GGG + chr4_2:27188371-27188390 None:intergenic 60.0%
CGTCCAACCTTAGGCGTGAG+TGG + chr4_2:27189431-27189450 None:intergenic 60.0%
! GACGGTTTGGAGCAGGAGGA+AGG - chr4_2:27190073-27190092 Msa0596610:intron 60.0%
AGGGGTCGTGGTTCGAACCC+TGG - chr4_2:27188659-27188678 Msa0596610:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 27186928 27190720 27186928 ID=Msa0596610;Name=Msa0596610
chr4_2 mRNA 27186928 27190720 27186928 ID=Msa0596610-mRNA-1;Parent=Msa0596610;Name=Msa0596610-mRNA-1;_AED=0.09;_eAED=0.09;_QI=326|0.75|0.6|1|0.25|0.4|5|0|323
chr4_2 exon 27186928 27187560 27186928 ID=Msa0596610-mRNA-1:exon:7207;Parent=Msa0596610-mRNA-1
chr4_2 exon 27188570 27188748 27188570 ID=Msa0596610-mRNA-1:exon:7206;Parent=Msa0596610-mRNA-1
chr4_2 exon 27189143 27189323 27189143 ID=Msa0596610-mRNA-1:exon:7205;Parent=Msa0596610-mRNA-1
chr4_2 exon 27190314 27190405 27190314 ID=Msa0596610-mRNA-1:exon:7204;Parent=Msa0596610-mRNA-1
chr4_2 exon 27190508 27190720 27190508 ID=Msa0596610-mRNA-1:exon:7203;Parent=Msa0596610-mRNA-1
chr4_2 five_prime_UTR 27190508 27190720 27190508 ID=Msa0596610-mRNA-1:five_prime_utr;Parent=Msa0596610-mRNA-1
chr4_2 five_prime_UTR 27190314 27190405 27190314 ID=Msa0596610-mRNA-1:five_prime_utr;Parent=Msa0596610-mRNA-1
chr4_2 five_prime_UTR 27189303 27189323 27189303 ID=Msa0596610-mRNA-1:five_prime_utr;Parent=Msa0596610-mRNA-1
chr4_2 CDS 27189143 27189302 27189143 ID=Msa0596610-mRNA-1:cds;Parent=Msa0596610-mRNA-1
chr4_2 CDS 27188570 27188748 27188570 ID=Msa0596610-mRNA-1:cds;Parent=Msa0596610-mRNA-1
chr4_2 CDS 27186928 27187560 27186928 ID=Msa0596610-mRNA-1:cds;Parent=Msa0596610-mRNA-1
Gene Sequence

>Msa0596610

ATGAATACTTTTTCTCATGTTCCCCCGGGGTTTAGGTTCCATCCCACAGATGAGGAACTTGTGGATTACTACCTTAGGAAGAAAATTGATTCAAGAAGGATTGATCTTGATGTCATAAGAGATGTTGACCTTTACAAAATTGAACCATGGGATCTTGAAGAGATTTGCAGAATAGGAACCGAAGAACAAAATGAGTGGTATTTTTTTAGCCATAAAGATAAGAAGTATCCAACTGGGACAAGAACAAATAGAGCAACTGCAGCTGGATTTTGGAAAGCAACAGGAAGAGACAAGGCAATCTATAGTAAGCATAATTTGATAGGAATGAGGAAAACATTGGAGGAAGGATGGGTTGTATGTAGAGTATTCAAGAAGAGAGTAACAACAACAATTCGCAAAATGAGTGAGCATGACTCTCCTTGTTGGTATGAAGATCAAGTTTCATTCATGCAAGATATGGACTCACCAAACCAAACCTCTCAATCCAATTTGATATACCAACAATTACCATCATATTCTTGCAAAAAAGAGCTAGATAATTTCCCATTCCAAAACATGTCTCGTGACAATTATCTTAATCTTCCACTTCTAGAGAGTCCAAAGCTTGTTCAATCTTCTTCAAACATAACCTCTAATCCAATGGTTACAACATATGACACTATAGATCATTCAAATTCTATGCTTCCTTCATCAATGCTCTTACAAGAAGAACAAGTTATACAAAGTGGTAATCAACAAAACTTTCATGCTATGTATGGAAACAATTCCAATGTTGAACAAGGTATGGTTGATGATCAAGTTACTGATTGGAGAGTTCTTGACAAGTTTGTTGCTTCACAACTTAGTCAAGAAGATGCATCTAAAGTGAATAATAACAACATCTTTCATGGCAATATTGAATTCAGAAATTTGGAGAAACAAGAAATGGTGCCTCATGAAAATGCCTCTACATCAAACACAAGTTGGATATGA
Protein sequence

>Msa0596610

MNTFSHVPPGFRFHPTDEELVDYYLRKKIDSRRIDLDVIRDVDLYKIEPWDLEEICRIGTEEQNEWYFFSHKDKKYPTGTRTNRATAAGFWKATGRDKAIYSKHNLIGMRKTLEEGWVVCRVFKKRVTTTIRKMSEHDSPCWYEDQVSFMQDMDSPNQTSQSNLIYQQLPSYSCKKELDNFPFQNMSRDNYLNLPLLESPKLVQSSSNITSNPMVTTYDTIDHSNSMLPSSMLLQEEQVIQSGNQQNFHAMYGNNSNVEQGMVDDQVTDWRVLDKFVASQLSQEDASKVNNNNIFHGNIEFRNLEKQEMVPHENASTSNTSWI*