AlfalfaGEDB | Msa1299980

M. sativa cultivar zhongmu-4 / Msa1299980

KEGG: -
GO: GO:0001871 GO:0001872 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009505 GO:0009506 GO:0009628 GO:0009987 GO:0016020 GO:0016043 GO:0016787 GO:0016798 GO:0030054 GO:0030246 GO:0030247 GO:0030312 GO:0031224 GO:0031225 GO:0031226 GO:0033036 GO:0033037 GO:0042545 GO:0044425 GO:0044459 GO:0044464 GO:0045229 GO:0046658 GO:0050896 GO:0051179 GO:0051641 GO:0052386 GO:0052543 GO:0052545 GO:0055044 GO:0070727 GO:0071554 GO:0071555 GO:0071840 GO:0071944
PFAM: PF07983.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299980	sp\|Q9FZ86\|PDCB3_ARATH	63.303	109	38	2	4	111	2	109	6.01e-42	143

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299980	A0A2Z6MGS9	72.146	219	43	3	14	228	14	218	3.39e-93	281

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0015570	Msa1299980	0.844543	7.083041e-59	-8.615850e-47
Msa0018920	Msa1299980	0.816393	5.660281e-52	-8.615850e-47
Msa0025160	Msa1299980	0.827314	1.674924e-54	-8.615850e-47
Msa0030070	Msa1299980	0.817933	2.550647e-52	-8.615850e-47
Msa0032970	Msa1299980	0.812682	3.750283e-51	-8.615850e-47
Msa0032980	Msa1299980	0.821074	4.899085e-53	-8.615850e-47
Msa0063190	Msa1299980	0.808025	3.793009e-50	-8.615850e-47
Msa0067060	Msa1299980	0.833612	4.826261e-56	-8.615850e-47
Msa0072520	Msa1299980	0.853757	1.934645e-61	-8.615850e-47
Msa0091350	Msa1299980	0.860198	2.441253e-63	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299980	MtrunA17_Chr4g0061401	91.628	215	8	1	14	228	14	218	1.26e-90	265
Msa1299980	MtrunA17_Chr5g0419121	59.633	109	41	2	4	109	2	110	1.60e-36	129
Msa1299980	MtrunA17_Chr4g0043151	56.757	111	43	4	1	109	1	108	1.55e-34	122
Msa1299980	MtrunA17_Chr8g0378361	55.789	95	41	1	21	114	27	121	1.73e-31	114
Msa1299980	MtrunA17_Chr4g0043511	39.623	106	63	1	22	126	418	523	6.74e-25	102
Msa1299980	MtrunA17_Chr8g0378371	45.045	111	55	3	1	109	10	116	7.95e-25	94.7
Msa1299980	MtrunA17_Chr8g0378381	41.667	108	61	2	1	107	10	116	2.24e-24	93.6
Msa1299980	MtrunA17_Chr8g0378401	41.121	107	61	2	1	106	10	115	3.36e-23	90.5
Msa1299980	MtrunA17_Chr3g0110371	46.154	104	54	2	24	125	361	464	3.88e-23	97.1
Msa1299980	MtrunA17_Chr5g0434591	47.959	98	49	2	20	115	356	453	7.26e-23	96.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299980	AT1G18650.1	63.303	109	38	2	4	111	2	109	6.11e-43	143
Msa1299980	AT1G18650.2	62.963	108	38	2	4	110	2	108	3.73e-42	140
Msa1299980	AT5G08000.2	47.429	175	86	3	6	174	22	196	4.83e-38	132
Msa1299980	AT5G61130.1	51.880	133	53	2	20	141	17	149	4.59e-36	126
Msa1299980	AT5G08000.1	49.682	157	73	3	20	170	17	173	1.90e-35	124
Msa1299980	AT4G13600.1	35.577	208	98	5	21	227	58	230	8.53e-31	114
Msa1299980	AT2G03505.4	51.613	93	42	2	20	109	17	109	3.60e-29	107
Msa1299980	AT2G03505.1	51.613	93	42	2	20	109	17	109	3.60e-29	107
Msa1299980	AT2G03505.2	51.613	93	42	2	20	109	17	109	3.60e-29	107
Msa1299980	AT2G03505.3	51.613	93	42	2	20	109	17	109	6.30e-29	105

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Find 119 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATAAACATAGATTAG+AGG	0.412614	8_3:+61379034	None:intergenic
AAACCTGGCACTTGAAGGGT+AGG	0.578796	8_3:+61378308	None:intergenic
AAATATAAACATAGATTAGA+GGG	0.572185	8_3:+61379035	None:intergenic
AACAGGCACAGGCACCGGCA+CGG	0.482270	8_3:-61375643	Msa1299980:CDS
AAGACCTTGGACTATGCTTG+TGG	0.615068	8_3:-61378616	Msa1299980:CDS
AATAGCTATTACCAGAAGAA+GGG	0.608399	8_3:-61378508	Msa1299980:CDS
AATCAGTATGGAAAAGGACT+TGG	0.466994	8_3:-61375179	Msa1299980:three_prime_UTR
AATGAGCTCTAACGGTGTTT+GGG	0.405145	8_3:+61378545	None:intergenic
AATTGGTGCGTGTGCAAGGA+TGG	0.547293	8_3:-61378658	Msa1299980:CDS
AATTTAAACCTGGCACTTGA+AGG	0.429774	8_3:+61378303	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAGATAGCAATGAAAAACG+TGG	+	chr8_3:61375165-61375184	None:intergenic	30.0%
TCTCTAACTTTGACATATGT+AGG	-	chr8_3:61375409-61375428	Msa1299980:CDS	30.0%
AATAGCTATTACCAGAAGAA+GGG	-	chr8_3:61375586-61375605	Msa1299980:CDS	30.0%
GGTGAAAATGAGTATTTACT+TGG	+	chr8_3:61375667-61375686	None:intergenic	30.0%
AAGAATCTTATGGACAATAG+TGG	-	chr8_3:61375894-61375913	Msa1299980:intron	30.0%
TAGACAATAGTAGTGTATAG+TGG	-	chr8_3:61376070-61376089	Msa1299980:intron	30.0%
TAATCAATTATGTGACCTAC+CGG	-	chr8_3:61376546-61376565	Msa1299980:intron	30.0%
ACATATTTGCACACAAGATA+GGG	+	chr8_3:61376604-61376623	None:intergenic	30.0%
TACATATTTGCACACAAGAT+AGG	+	chr8_3:61376605-61376624	None:intergenic	30.0%
ACAATACATACACGTTGAAT+TGG	+	chr8_3:61376854-61376873	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	61375054	61379062	61375054	ID=Msa1299980;Name=Msa1299980
chr8_3	mRNA	61375054	61379062	61375054	ID=Msa1299980-mRNA-1;Parent=Msa1299980;Name=Msa1299980-mRNA-1;_AED=0.07;_eAED=0.07;_QI=174\|1\|1\|1\|1\|1\|4\|341\|228
chr8_3	exon	61375054	61375749	61375054	ID=Msa1299980-mRNA-1:exon:18063;Parent=Msa1299980-mRNA-1
chr8_3	exon	61378312	61378345	61378312	ID=Msa1299980-mRNA-1:exon:18062;Parent=Msa1299980-mRNA-1
chr8_3	exon	61378449	61378685	61378449	ID=Msa1299980-mRNA-1:exon:18061;Parent=Msa1299980-mRNA-1
chr8_3	exon	61378828	61379062	61378828	ID=Msa1299980-mRNA-1:exon:18060;Parent=Msa1299980-mRNA-1
chr8_3	five_prime_UTR	61378889	61379062	61378889	ID=Msa1299980-mRNA-1:five_prime_utr;Parent=Msa1299980-mRNA-1
chr8_3	CDS	61378828	61378888	61378828	ID=Msa1299980-mRNA-1:cds;Parent=Msa1299980-mRNA-1
chr8_3	CDS	61378449	61378685	61378449	ID=Msa1299980-mRNA-1:cds;Parent=Msa1299980-mRNA-1
chr8_3	CDS	61378312	61378345	61378312	ID=Msa1299980-mRNA-1:cds;Parent=Msa1299980-mRNA-1
chr8_3	CDS	61375395	61375749	61375395	ID=Msa1299980-mRNA-1:cds;Parent=Msa1299980-mRNA-1
chr8_3	three_prime_UTR	61375054	61375394	61375054	ID=Msa1299980-mRNA-1:three_prime_utr;Parent=Msa1299980-mRNA-1

Gene Sequence

>Msa1299980

ATGGCTTTTGCTACTCTTGCTCTTGTACTTCTTCTTTTGCCCTTCACCGCCATTCCTTCAAGTGCTAATTGGTGCGTGTGCAAGGATGGAGCTGATGCAATCTTACAAAAGACCTTGGACTATGCTTGTGGAGCTGGAGCTGACTGTAACCCTCTTCGTCAAAACGCTCCTTGTTACAACCCAAACACCGTTAGAGCTCATTGCAGCTATGCTGTGAATAGCTATTACCAGAAGAAGGGTCAACAAGCACTGGCTTGTGACTTTGCAGGAACAGCTACTGTTGTTACATCTGATCCAAGTGTTAGTGGCTGTGCCTACCCTTCAAGTGCCAGTGGTTCAGGCACTAGCACAACAAGCCCATCGACAGGTACCGGAACAGGTACCGGAACAGGCACCGGCATGGGAACCACCCCGTCAACAGGCACAGGCACCGGCACGGGCACCGGCACTGGCACAGGCACCAGCACGGGCACCGGCATGGGAACTGGCACCAGCACCGGCGCCACTGGTAATACTCCATACAGTACAACAGCTCCTGGTGGTGTGTTAGGAGGTATAGGCAGTGGTATGGGGCCTTCTGGAGCTGGAGGAATGAATGATGATAGTCATGGTGGTCTTAGACTTGTGGACACTAGTTTCTTATCAATCCCTCTCTTTTCTGTCTTTATTATGTTTTGGTGGGGTTAA

Protein sequence

>Msa1299980

MAFATLALVLLLLPFTAIPSSANWCVCKDGADAILQKTLDYACGAGADCNPLRQNAPCYNPNTVRAHCSYAVNSYYQKKGQQALACDFAGTATVVTSDPSVSGCAYPSSASGSGTSTTSPSTGTGTGTGTGTGMGTTPSTGTGTGTGTGTGTGTSTGTGMGTGTSTGATGNTPYSTTAPGGVLGGIGSGMGPSGAGGMNDDSHGGLRLVDTSFLSIPLFSVFIMFWWG*