AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0699240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0699240 MtrunA17_Chr4g0052931 98.765 81 1 0 1 81 1 81 5.78e-55 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0699240 AT4G20150.1 74.074 81 21 0 1 81 1 81 3.43e-41 130

Find 33 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAGCTTGTTGATAAAAT+AGG 0.169095 4_4:+69847928 None:intergenic
TGCCATGAGTTTGGTAATTT+TGG 0.185078 4_4:-69847773 Msa0699240:three_prime_UTR
ATCGGAGCCTTATTAGGTTT+GGG 0.248368 4_4:-69850790 Msa0699240:CDS
CTGAGTCCCCAAACCTAATA+AGG 0.285931 4_4:+69850783 None:intergenic
CCGTCAATCAGCTTCTTAAA+TGG 0.301085 4_4:-69850581 Msa0699240:CDS
AGATCCGTGGGAGCACGTTT+TGG 0.314042 4_4:-69850625 Msa0699240:CDS
TAGATGATGTTATGAACTTA+TGG 0.318632 4_4:-69847890 Msa0699240:three_prime_UTR
GATCGGAGCCTTATTAGGTT+TGG 0.322875 4_4:-69850791 Msa0699240:CDS
AACCTAAAATTAGTTTCATA+AGG 0.362085 4_4:-69847708 None:intergenic
ACGGCGATCGGAGCCTTATT+AGG 0.378847 4_4:-69850796 Msa0699240:CDS
ACGATTAGTGCATGTCTTCA+AGG 0.414627 4_4:-69848067 Msa0699240:exon
TTCAATGCATGCCATGAGTT+TGG 0.430341 4_4:-69847782 Msa0699240:three_prime_UTR
GATAAAATAGGTTGATTAGC+AGG 0.438366 4_4:+69847940 None:intergenic
CCATTTAAGAAGCTGATTGA+CGG 0.458422 4_4:+69850581 None:intergenic
CATTGAACACATTAGATAAG+AGG 0.462591 4_4:+69847798 None:intergenic
CGTCAATCAGCTTCTTAAAT+GGG 0.464581 4_4:-69850580 Msa0699240:CDS
AGATGTACTCCAACGCTCTC+AGG 0.466035 4_4:-69850762 Msa0699240:CDS
GATGAATTACGACGCATGTA+AGG 0.488766 4_4:+69850733 None:intergenic
GTGCTAAGCGCGACGGCGAT+CGG 0.497668 4_4:-69850808 Msa0699240:CDS
TGCAATTGTGTTAGATCCGT+GGG 0.502929 4_4:-69850637 Msa0699240:intron
AATGTAACGTCTTTCATTAG+CGG 0.520069 4_4:+69850509 None:intergenic
TCGGAGCCTTATTAGGTTTG+GGG 0.521009 4_4:-69850789 Msa0699240:CDS
AAGGATTGCGTTGCAGCAGT+TGG 0.529059 4_4:-69848048 Msa0699240:three_prime_UTR
GGGAATGGGATTGGGCGTTG+TGG 0.541699 4_4:-69850604 Msa0699240:CDS
CACCAAAATTACCAAACTCA+TGG 0.543837 4_4:+69847771 None:intergenic
AGGAAGTTTCCTGAGAGCGT+TGG 0.556266 4_4:+69850753 None:intergenic
ATTCCCAAAACGTGCTCCCA+CGG 0.568376 4_4:+69850621 None:intergenic
TTGCAATTGTGTTAGATCCG+TGG 0.576057 4_4:-69850638 Msa0699240:intron
GAGAACAACATAGTTTAGAA+CGG 0.600699 4_4:-69850872 Msa0699240:five_prime_UTR
GCTAATGAAAGACGTTACAT+TGG 0.602826 4_4:-69850507 Msa0699240:intron
TAAGATGCTTGACAAAGCTA+AGG 0.619792 4_4:-69850532 Msa0699240:CDS
CAATCAGCTTCTTAAATGGG+AGG 0.632016 4_4:-69850577 Msa0699240:CDS
AACAAGAAATCATAAAACAA+AGG 0.646730 4_4:+69847992 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGACATTTATTTTTTTTT+TGG + chr4_4:69850243-69850262 None:intergenic 10.0%
!! AAAAAAAAAATAAATGTCTT+TGG - chr4_4:69850242-69850261 Msa0699240:intron 10.0%
!! TTATAAACCAAAAAATTTAT+CGG + chr4_4:69850748-69850767 None:intergenic 10.0%
!!! ATTAATATTTTACAAATGTT+TGG + chr4_4:69850858-69850877 None:intergenic 10.0%
!! AACAATATAATAAATAAGCA+CGG + chr4_4:69850484-69850503 None:intergenic 15.0%
!!! TGTAATTATGATGATGATTT+AGG - chr4_4:69848196-69848215 Msa0699240:intron 20.0%
!!! TTTATCTTCTGAAATAAGTT+TGG + chr4_4:69848468-69848487 None:intergenic 20.0%
!! TATTTCAGAAGATAAACTTA+TGG - chr4_4:69848472-69848491 Msa0699240:intron 20.0%
!! AAGAAGATGAAAAAATGTAT+TGG - chr4_4:69848562-69848581 Msa0699240:intron 20.0%
!! AGAAGATGAAAAAATGTATT+GGG - chr4_4:69848563-69848582 Msa0699240:intron 20.0%
!! TACAATATACATAGTCAATA+CGG + chr4_4:69848634-69848653 None:intergenic 20.0%
!! ATAAGCATATTATCAATATC+AGG + chr4_4:69849050-69849069 None:intergenic 20.0%
!! AATTAAAACAAACAAGATCT+AGG + chr4_4:69849376-69849395 None:intergenic 20.0%
!!! AAAAATTTGTTACCATATTG+AGG - chr4_4:69849836-69849855 Msa0699240:intron 20.0%
!!! TTTAAAACTTAAAAAGACCT+AGG - chr4_4:69849929-69849948 Msa0699240:intron 20.0%
!! AAATCAAAATCACAGTATTT+GGG + chr4_4:69850025-69850044 None:intergenic 20.0%
!! AAAATCAAAATCACAGTATT+TGG + chr4_4:69850026-69850045 None:intergenic 20.0%
!! AGATAAATCACATTAATAAG+TGG + chr4_4:69850107-69850126 None:intergenic 20.0%
!! AAAAAAATAAATGTCTTTGG+TGG - chr4_4:69850245-69850264 Msa0699240:intron 20.0%
!! CATAAAATACTAAAATGACA+GGG + chr4_4:69850330-69850349 None:intergenic 20.0%
!!! TAGTATTTTATGGTAATTTC+TGG - chr4_4:69850338-69850357 Msa0699240:intron 20.0%
!!! TTATATTGTTTTATTGCAGA+TGG - chr4_4:69850494-69850513 Msa0699240:intron 20.0%
!! AACAAGAAATCATAAAACAA+AGG + chr4_4:69850600-69850619 None:intergenic 20.0%
!!! AATGAATCCGATAAATTTTT+TGG - chr4_4:69850738-69850757 Msa0699240:intron 20.0%
!!! ATATTTTACAAATGTTTGGT+CGG + chr4_4:69850854-69850873 None:intergenic 20.0%
!!! GTTTTGATTTTGAGTAACTT+CGG - chr4_4:69848173-69848192 Msa0699240:intron 25.0%
!!! AAGATTTTGTTTGTTTCTGA+TGG - chr4_4:69848795-69848814 Msa0699240:intron 25.0%
! GATACAGACAACTAAATTAA+AGG + chr4_4:69848914-69848933 None:intergenic 25.0%
! TTTCTTTAACTCTTGTTACT+AGG - chr4_4:69848940-69848959 Msa0699240:intron 25.0%
!! TTAACGTGAATTCTGAAAAA+TGG + chr4_4:69848971-69848990 None:intergenic 25.0%
!!! AAGAAAGAAGACAGATTTTT+TGG + chr4_4:69849120-69849139 None:intergenic 25.0%
! TCTAATAGTCTTTCTTAACT+TGG + chr4_4:69849249-69849268 None:intergenic 25.0%
! AGAAAGACTATTAGAAGAAT+TGG - chr4_4:69849254-69849273 Msa0699240:intron 25.0%
! GAAAGACTATTAGAAGAATT+GGG - chr4_4:69849255-69849274 Msa0699240:intron 25.0%
! ATGCCACAAATATGAAAATA+GGG + chr4_4:69849301-69849320 None:intergenic 25.0%
!!! GTTTTAGTTTAGTTTTCTGT+TGG - chr4_4:69849399-69849418 Msa0699240:intron 25.0%
! TTGTTTATCTACACCATAAT+AGG - chr4_4:69849428-69849447 Msa0699240:intron 25.0%
!!! TTTCATGGTAGATGTTTTTA+TGG - chr4_4:69849501-69849520 Msa0699240:intron 25.0%
!! TTACAAAGAGCATATTTAAC+AGG + chr4_4:69849566-69849585 None:intergenic 25.0%
!! GCATGATTTATTCAGTTTTT+CGG - chr4_4:69849702-69849721 Msa0699240:intron 25.0%
! ATTAAAACTAAAAACCTACG+TGG + chr4_4:69849879-69849898 None:intergenic 25.0%
!!! ACGTAGGTTTTTAGTTTTAA+TGG - chr4_4:69849878-69849897 Msa0699240:intron 25.0%
!!! GTTTTAATGGAAACTCTAAA+AGG - chr4_4:69849891-69849910 Msa0699240:intron 25.0%
! GTACAATCAAACATCTTAAT+GGG - chr4_4:69849982-69850001 Msa0699240:intron 25.0%
!!! TTTGGATTTGTTGTAATTCA+CGG - chr4_4:69850061-69850080 Msa0699240:intron 25.0%
! TTTAGCTGTAATAATGTCTT+TGG - chr4_4:69850205-69850224 Msa0699240:intron 25.0%
! CCATAAAATACTAAAATGAC+AGG + chr4_4:69850331-69850350 None:intergenic 25.0%
!!! CCTGTCATTTTAGTATTTTA+TGG - chr4_4:69850328-69850347 Msa0699240:intron 25.0%
! TAGATGATGTTATGAACTTA+TGG - chr4_4:69850699-69850718 Msa0699240:intron 25.0%
GAGAACAACATAGTTTAGAA+CGG - chr4_4:69847717-69847736 Msa0699240:three_prime_UTR 30.0%
AATGTAACGTCTTTCATTAG+CGG + chr4_4:69848083-69848102 None:intergenic 30.0%
!! ATTTAGGATGATTTGTTCTG+AGG - chr4_4:69848212-69848231 Msa0699240:intron 30.0%
AATAAGTCGTTTATCCAAAC+AGG + chr4_4:69848274-69848293 None:intergenic 30.0%
AACGACTTATTTGTAGCTTA+TGG - chr4_4:69848283-69848302 Msa0699240:intron 30.0%
ATATGAAAACAGCTTATGAC+AGG + chr4_4:69848388-69848407 None:intergenic 30.0%
TATGGAAGAGAATCTATTTG+TGG - chr4_4:69848490-69848509 Msa0699240:intron 30.0%
CTATCTGGCATTAGAAATTT+AGG - chr4_4:69848533-69848552 Msa0699240:intron 30.0%
GATGAAAAAATGTATTGGGT+AGG - chr4_4:69848567-69848586 Msa0699240:intron 30.0%
ACACAATTCTCTTCAATATG+TGG + chr4_4:69848708-69848727 None:intergenic 30.0%
!!! AGTATGGTGTTTTAAGTTAG+TGG - chr4_4:69848753-69848772 Msa0699240:intron 30.0%
ACTAGGCAAAATTGTATTTG+TGG - chr4_4:69848887-69848906 Msa0699240:intron 30.0%
TAGAAGTGGGGAAAAAATTA+GGG + chr4_4:69849153-69849172 None:intergenic 30.0%
ATAGAAGTGGGGAAAAAATT+AGG + chr4_4:69849154-69849173 None:intergenic 30.0%
!!! GAGAAAACAATTTTGGTTCA+AGG + chr4_4:69849177-69849196 None:intergenic 30.0%
!!! ATAAGAAGCCTGTTTTGTTT+TGG + chr4_4:69849203-69849222 None:intergenic 30.0%
! ATAGTCTTTCTTAACTTGGT+GGG + chr4_4:69849245-69849264 None:intergenic 30.0%
! AATAGTCTTTCTTAACTTGG+TGG + chr4_4:69849246-69849265 None:intergenic 30.0%
! TACCCCTATTTTCATATTTG+TGG - chr4_4:69849295-69849314 Msa0699240:intron 30.0%
TGCCACAAATATGAAAATAG+GGG + chr4_4:69849300-69849319 None:intergenic 30.0%
GATGCCACAAATATGAAAAT+AGG + chr4_4:69849302-69849321 None:intergenic 30.0%
TTTCATATTTGTGGCATCAT+TGG - chr4_4:69849304-69849323 Msa0699240:intron 30.0%
TTTGGGAACAAACTAAACTA+AGG + chr4_4:69849352-69849371 None:intergenic 30.0%
! ACAAACAAGATCTAGGTTTT+GGG + chr4_4:69849369-69849388 None:intergenic 30.0%
! AACAAACAAGATCTAGGTTT+TGG + chr4_4:69849370-69849389 None:intergenic 30.0%
! CACCATAATAGGTTTTTGAT+TGG - chr4_4:69849439-69849458 Msa0699240:intron 30.0%
CTCCAATCAAAAACCTATTA+TGG + chr4_4:69849444-69849463 None:intergenic 30.0%
!! GGTAGATGTTTTTATGGATT+TGG - chr4_4:69849507-69849526 Msa0699240:intron 30.0%
!! TTTTATGGATTTGGTAGAAG+TGG - chr4_4:69849516-69849535 Msa0699240:intron 30.0%
TCTTTGTAACTAGTTGCTAA+TGG - chr4_4:69849577-69849596 Msa0699240:intron 30.0%
CTTTGTAACTAGTTGCTAAT+GGG - chr4_4:69849578-69849597 Msa0699240:intron 30.0%
TCTGTTTAGACACACATAAA+TGG - chr4_4:69849680-69849699 Msa0699240:intron 30.0%
GATGTTTATCATCCTCAATA+TGG + chr4_4:69849851-69849870 None:intergenic 30.0%
AAAACTTAAAAAGACCTAGG+TGG - chr4_4:69849932-69849951 Msa0699240:intron 30.0%
AAACTTAAAAAGACCTAGGT+GGG - chr4_4:69849933-69849952 Msa0699240:intron 30.0%
GGTACAATCAAACATCTTAA+TGG - chr4_4:69849981-69850000 Msa0699240:intron 30.0%
!! TTTAAGCATTGACATGACTT+TGG - chr4_4:69850043-69850062 Msa0699240:intron 30.0%
!! TTGGATTTGTTGTAATTCAC+GGG - chr4_4:69850062-69850081 Msa0699240:intron 30.0%
ACAGGGAAAAAAAATTGACT+TGG + chr4_4:69850313-69850332 None:intergenic 30.0%
!! GATAAAATAGGTTGATTAGC+AGG + chr4_4:69850652-69850671 None:intergenic 30.0%
!! CTGAAGCTTGTTGATAAAAT+AGG + chr4_4:69850664-69850683 None:intergenic 30.0%
! CTATTTTATCAACAAGCTTC+AGG - chr4_4:69850662-69850681 Msa0699240:intron 30.0%
CATTGAACACATTAGATAAG+AGG + chr4_4:69850794-69850813 None:intergenic 30.0%
CCATTTAAGAAGCTGATTGA+CGG + chr4_4:69848011-69848030 None:intergenic 35.0%
CGTCAATCAGCTTCTTAAAT+GGG - chr4_4:69848009-69848028 Msa0699240:three_prime_UTR 35.0%
TAAGATGCTTGACAAAGCTA+AGG - chr4_4:69848057-69848076 Msa0699240:three_prime_UTR 35.0%
GCTAATGAAAGACGTTACAT+TGG - chr4_4:69848082-69848101 Msa0699240:intron 35.0%
!! ATGATTTGTTCTGAGGAAAC+TGG - chr4_4:69848219-69848238 Msa0699240:intron 35.0%
GTTTGGTTGATTGTGCATTA+GGG - chr4_4:69848248-69848267 Msa0699240:intron 35.0%
TAAGCTTGCATAAGCTGTAT+TGG - chr4_4:69848325-69848344 Msa0699240:intron 35.0%
! TAAGTATCGTGAGACTGTTT+GGG + chr4_4:69848416-69848435 None:intergenic 35.0%
! ATAAGTATCGTGAGACTGTT+TGG + chr4_4:69848417-69848436 None:intergenic 35.0%
ACTAATCTGAGCTTATCTTC+AGG + chr4_4:69848441-69848460 None:intergenic 35.0%
CTATTTGTGGAATCAAGCAA+TGG - chr4_4:69848503-69848522 Msa0699240:intron 35.0%
AGCAATGGTATATTGCTATC+TGG - chr4_4:69848518-69848537 Msa0699240:intron 35.0%
TCTTCATGTACCCAATACTT+GGG - chr4_4:69848657-69848676 Msa0699240:intron 35.0%
! ACTATACACTTTTGAGCTTG+AGG - chr4_4:69848859-69848878 Msa0699240:intron 35.0%
ACGTTAAAAAAATACGCACG+TGG - chr4_4:69848984-69849003 Msa0699240:intron 35.0%
AATCCACATTAGACCCATAA+CGG + chr4_4:69849010-69849029 None:intergenic 35.0%
! TTTGGTTCAAGGATAGAAGT+GGG + chr4_4:69849166-69849185 None:intergenic 35.0%
!!! TTTTGGTTCAAGGATAGAAG+TGG + chr4_4:69849167-69849186 None:intergenic 35.0%
! TTGGCTGGAGAAAACAATTT+TGG + chr4_4:69849184-69849203 None:intergenic 35.0%
! TCTTAACTTGGTGGGAAAAA+GGG + chr4_4:69849237-69849256 None:intergenic 35.0%
! TTCTTAACTTGGTGGGAAAA+AGG + chr4_4:69849238-69849257 None:intergenic 35.0%
CTAAACTAAGGAAGGCAAAA+TGG + chr4_4:69849340-69849359 None:intergenic 35.0%
GGAACAAACTAAACTAAGGA+AGG + chr4_4:69849348-69849367 None:intergenic 35.0%
TAACTAGTTGCTAATGGGAT+TGG - chr4_4:69849583-69849602 Msa0699240:intron 35.0%
!! AAGATGAAGAGGAGTGTTAT+TGG - chr4_4:69849767-69849786 Msa0699240:intron 35.0%
! TGGAAACTCTAAAAGGTCAA+AGG - chr4_4:69849898-69849917 Msa0699240:intron 35.0%
AAAACCTTATTGGACCTATC+TGG - chr4_4:69849960-69849979 Msa0699240:intron 35.0%
GATGTTTGATTGTACCAGAT+AGG + chr4_4:69849977-69849996 None:intergenic 35.0%
!! TGGATTTGTTGTAATTCACG+GGG - chr4_4:69850063-69850082 Msa0699240:intron 35.0%
ATAAGTGGAACTAATCACCT+AGG + chr4_4:69850092-69850111 None:intergenic 35.0%
CACACATTAAGAACGAATGA+AGG + chr4_4:69850171-69850190 None:intergenic 35.0%
AGCTGTAATAATGTCTTTGG+TGG - chr4_4:69850208-69850227 Msa0699240:intron 35.0%
! GTCTTTGCACGAAATGTTTT+TGG - chr4_4:69850388-69850407 Msa0699240:intron 35.0%
!! TGCCATGAGTTTGGTAATTT+TGG - chr4_4:69850816-69850835 Msa0699240:exon 35.0%
CACCAAAATTACCAAACTCA+TGG + chr4_4:69850821-69850840 None:intergenic 35.0%
GAGAGAGAGAGGAAGAAAAA+TGG - chr4_4:69847759-69847778 Msa0699240:three_prime_UTR 40.0%
!! ATCGGAGCCTTATTAGGTTT+GGG - chr4_4:69847799-69847818 Msa0699240:three_prime_UTR 40.0%
GATGAATTACGACGCATGTA+AGG + chr4_4:69847859-69847878 None:intergenic 40.0%
TTGCAATTGTGTTAGATCCG+TGG - chr4_4:69847951-69847970 Msa0699240:three_prime_UTR 40.0%
TGCAATTGTGTTAGATCCGT+GGG - chr4_4:69847952-69847971 Msa0699240:three_prime_UTR 40.0%
CCGTCAATCAGCTTCTTAAA+TGG - chr4_4:69848008-69848027 Msa0699240:three_prime_UTR 40.0%
CAATCAGCTTCTTAAATGGG+AGG - chr4_4:69848012-69848031 Msa0699240:three_prime_UTR 40.0%
!! TGTTCTGAGGAAACTGGAAT+TGG - chr4_4:69848225-69848244 Msa0699240:intron 40.0%
!! GTTCTGAGGAAACTGGAATT+GGG - chr4_4:69848226-69848245 Msa0699240:intron 40.0%
! GGTTTGGTTGATTGTGCATT+AGG - chr4_4:69848247-69848266 Msa0699240:intron 40.0%
CTCTTCATGTACCCAATACT+TGG - chr4_4:69848656-69848675 Msa0699240:intron 40.0%
!! ACTTGCTATGCAGAAGAGTA+TGG - chr4_4:69848737-69848756 Msa0699240:intron 40.0%
!!! GTGTTTTAAGTTAGTGGCGT+GGG - chr4_4:69848759-69848778 Msa0699240:intron 40.0%
TGGATTAAACGACTCTGATG+TGG - chr4_4:69849024-69849043 Msa0699240:intron 40.0%
TTGGTTCAAGGATAGAAGTG+GGG + chr4_4:69849165-69849184 None:intergenic 40.0%
TTCTCCAGCCAAAACAAAAC+AGG - chr4_4:69849192-69849211 Msa0699240:intron 40.0%
TGGTGGGAAAAAGGGAAAAT+TGG + chr4_4:69849229-69849248 None:intergenic 40.0%
AACTAAGGAAGGCAAAATGG+AGG + chr4_4:69849337-69849356 None:intergenic 40.0%
CTGAGAAACATCAGCTTTCA+TGG - chr4_4:69849486-69849505 Msa0699240:intron 40.0%
ACAGGTGACTAATTACACCA+AGG + chr4_4:69849548-69849567 None:intergenic 40.0%
!! TTGCTAATGGGATTGGTCAT+TGG - chr4_4:69849590-69849609 Msa0699240:intron 40.0%
TAAATGTTGTGTTGTGCGAG+TGG - chr4_4:69849744-69849763 Msa0699240:intron 40.0%
!! AAGAGGAGTGTTATTGGAGA+AGG - chr4_4:69849773-69849792 Msa0699240:intron 40.0%
!! AGAGGAGTGTTATTGGAGAA+GGG - chr4_4:69849774-69849793 Msa0699240:intron 40.0%
ATAAACATCAACAGCCACGT+AGG - chr4_4:69849862-69849881 Msa0699240:intron 40.0%
!!! TAAGGTTTTTTGTCCCACCT+AGG + chr4_4:69849949-69849968 None:intergenic 40.0%
GGTGGGACAAAAAACCTTAT+TGG - chr4_4:69849950-69849969 Msa0699240:intron 40.0%
TGTACCAGATAGGTCCAATA+AGG + chr4_4:69849967-69849986 None:intergenic 40.0%
!!! GCTTTGGATGTGGTGTTTTT+TGG - chr4_4:69850437-69850456 Msa0699240:intron 40.0%
ACGATTAGTGCATGTCTTCA+AGG - chr4_4:69850522-69850541 Msa0699240:CDS 40.0%
TTCAATGCATGCCATGAGTT+TGG - chr4_4:69850807-69850826 Msa0699240:CDS 40.0%
TCTGAGAAAGAGAGAGAGAG+AGG - chr4_4:69847748-69847767 Msa0699240:three_prime_UTR 45.0%
! AAAAATGGTGCTAAGCGCGA+CGG - chr4_4:69847774-69847793 Msa0699240:three_prime_UTR 45.0%
!! GATCGGAGCCTTATTAGGTT+TGG - chr4_4:69847798-69847817 Msa0699240:three_prime_UTR 45.0%
!! TCGGAGCCTTATTAGGTTTG+GGG - chr4_4:69847800-69847819 Msa0699240:three_prime_UTR 45.0%
CTGAGTCCCCAAACCTAATA+AGG + chr4_4:69847809-69847828 None:intergenic 45.0%
!!! CACGTTTTGGGAATGGGATT+GGG - chr4_4:69847977-69847996 Msa0699240:three_prime_UTR 45.0%
GAGGAAACTGGAATTGGGTT+TGG - chr4_4:69848231-69848250 Msa0699240:intron 45.0%
ATTGTGCATTAGGGCCTGTT+TGG - chr4_4:69848257-69848276 Msa0699240:intron 45.0%
TCATGTACCCAATACTTGGG+AGG - chr4_4:69848660-69848679 Msa0699240:intron 45.0%
CATGTACCCAATACTTGGGA+GGG - chr4_4:69848661-69848680 Msa0699240:intron 45.0%
ATGTACCCAATACTTGGGAG+GGG - chr4_4:69848662-69848681 Msa0699240:intron 45.0%
!!! GGTGTTTTAAGTTAGTGGCG+TGG - chr4_4:69848758-69848777 Msa0699240:intron 45.0%
TTGAGCTTGAGGTCGATACT+AGG - chr4_4:69848870-69848889 Msa0699240:intron 45.0%
!!! GAAGCCTGTTTTGTTTTGGC+TGG + chr4_4:69849199-69849218 None:intergenic 45.0%
GGTAGAAGTGGTTACATCCT+TGG - chr4_4:69849528-69849547 Msa0699240:intron 45.0%
!! CAGTTTTTCGGTGTGAGTGA+TGG - chr4_4:69849714-69849733 Msa0699240:intron 45.0%
TGTAATTCACGGGGAAACCT+AGG - chr4_4:69850072-69850091 Msa0699240:intron 45.0%
GCTTCTGTAGCTGAATGCTT+TGG - chr4_4:69850421-69850440 Msa0699240:intron 45.0%
! GTAGCTGAATGCTTTGGATG+TGG - chr4_4:69850427-69850446 Msa0699240:intron 45.0%
AGATGTACTCCAACGCTCTC+AGG - chr4_4:69847827-69847846 Msa0699240:three_prime_UTR 50.0%
! AGGAAGTTTCCTGAGAGCGT+TGG + chr4_4:69847839-69847858 None:intergenic 50.0%
ATTCCCAAAACGTGCTCCCA+CGG + chr4_4:69847971-69847990 None:intergenic 50.0%
!!! TGGGAGCACGTTTTGGGAAT+GGG - chr4_4:69847971-69847990 Msa0699240:three_prime_UTR 50.0%
!!! GCACGTTTTGGGAATGGGAT+TGG - chr4_4:69847976-69847995 Msa0699240:three_prime_UTR 50.0%
AGTTAGTGGCGTGGGTAACA+TGG - chr4_4:69848767-69848786 Msa0699240:intron 50.0%
AAATACGCACGTGGCCGTTA+TGG - chr4_4:69848993-69849012 Msa0699240:intron 50.0%
AATACGCACGTGGCCGTTAT+GGG - chr4_4:69848994-69849013 Msa0699240:intron 50.0%
TGGCCGTTATGGGTCTAATG+TGG - chr4_4:69849004-69849023 Msa0699240:intron 50.0%
TGTGCGAGTGGAAGATGAAG+AGG - chr4_4:69849756-69849775 Msa0699240:intron 50.0%
AAGGATTGCGTTGCAGCAGT+TGG - chr4_4:69850541-69850560 Msa0699240:CDS 50.0%
!! ACGGCGATCGGAGCCTTATT+AGG - chr4_4:69847793-69847812 Msa0699240:three_prime_UTR 55.0%
!! AGATCCGTGGGAGCACGTTT+TGG - chr4_4:69847964-69847983 Msa0699240:three_prime_UTR 55.0%
!! GATCCGTGGGAGCACGTTTT+GGG - chr4_4:69847965-69847984 Msa0699240:three_prime_UTR 55.0%
!!! GTGGGAGCACGTTTTGGGAA+TGG - chr4_4:69847970-69847989 Msa0699240:three_prime_UTR 55.0%
CACTGCCCCTCCCAAGTATT+GGG + chr4_4:69848670-69848689 None:intergenic 55.0%
ACACTGCCCCTCCCAAGTAT+TGG + chr4_4:69848671-69848690 None:intergenic 55.0%
! GGGAATGGGATTGGGCGTTG+TGG - chr4_4:69847985-69848004 Msa0699240:three_prime_UTR 60.0%
GTGCTAAGCGCGACGGCGAT+CGG - chr4_4:69847781-69847800 Msa0699240:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 69847709 69850902 69847709 ID=Msa0699240;Name=Msa0699240
chr4_4 mRNA 69847709 69850902 69847709 ID=Msa0699240-mRNA-1;Parent=Msa0699240;Name=Msa0699240-mRNA-1;_AED=0.03;_eAED=0.03;_QI=69|1|1|1|1|1|3|373|81
chr4_4 exon 69847709 69848098 69847709 ID=Msa0699240-mRNA-1:exon:21024;Parent=Msa0699240-mRNA-1
chr4_4 exon 69850508 69850645 69850508 ID=Msa0699240-mRNA-1:exon:21023;Parent=Msa0699240-mRNA-1
chr4_4 exon 69850743 69850902 69850743 ID=Msa0699240-mRNA-1:exon:21022;Parent=Msa0699240-mRNA-1
chr4_4 five_prime_UTR 69850834 69850902 69850834 ID=Msa0699240-mRNA-1:five_prime_utr;Parent=Msa0699240-mRNA-1
chr4_4 CDS 69850743 69850833 69850743 ID=Msa0699240-mRNA-1:cds;Parent=Msa0699240-mRNA-1
chr4_4 CDS 69850508 69850645 69850508 ID=Msa0699240-mRNA-1:cds;Parent=Msa0699240-mRNA-1
chr4_4 CDS 69848082 69848098 69848082 ID=Msa0699240-mRNA-1:cds;Parent=Msa0699240-mRNA-1
chr4_4 three_prime_UTR 69847709 69848081 69847709 ID=Msa0699240-mRNA-1:three_prime_utr;Parent=Msa0699240-mRNA-1
Gene Sequence

>Msa0699240

ATGGTGCTAAGCGCGACGGCGATCGGAGCCTTATTAGGTTTGGGGACTCAGATGTACTCCAACGCTCTCAGGAAACTTCCTTACATGCGTCATCCGTGGGAGCACGTTTTGGGAATGGGATTGGGCGTTGTGGCCGTCAATCAGCTTCTTAAATGGGAGGCTCAAGTTGAACAAGATCTTGATAAGATGCTTGACAAAGCTAAGGCCGCTAATGAAAGACGTTACATTGATGGAGATGACGATTAG
Protein sequence

>Msa0699240

MVLSATAIGALLGLGTQMYSNALRKLPYMRHPWEHVLGMGLGVVAVNQLLKWEAQVEQDLDKMLDKAKAANERRYIDGDDD*