AlfalfaGEDB | Msa0142330

M. sativa cultivar zhongmu-4 / Msa0142330

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005747 GO:0008150 GO:0008152 GO:0009853 GO:0009987 GO:0016020 GO:0019866 GO:0030964 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045271 GO:0070469 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1902494 GO:1990204
PFAM: PF08122.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142330	GAU13148.1	85.075	67	10	0	4	70	3	69	1.45e-34	122

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142330	sp\|Q9M9R9\|NDB3B_ARATH	71.186	59	16	1	7	64	7	65	2.09e-25	92.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142330	A0A2Z6LH99	85.075	67	10	0	4	70	3	69	6.93e-35	122

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0082280	Msa0142330	0.849562	2.986810e-60	-8.615850e-47
Msa0142330	Msa0174770	0.816284	5.985746e-52	-8.615850e-47
Msa0142330	Msa0310030	0.801754	7.756221e-49	-8.615850e-47
Msa0142330	Msa0322890	0.825813	3.819159e-54	-8.615850e-47
Msa0142330	Msa0427150	0.822803	1.947977e-53	-8.615850e-47
Msa0142330	Msa0432210	0.811364	7.266334e-51	-8.615850e-47
Msa0142330	Msa0566700	0.839294	1.727465e-57	-8.615850e-47
Msa0142330	Msa0581070	0.818502	1.895941e-52	-8.615850e-47
Msa0142330	Msa0643980	0.800102	1.687358e-48	-8.615850e-47
Msa0142330	Msa0699240	0.883319	4.962397e-71	-8.615850e-47
Msa0142330	Msa0925440	0.801453	8.940840e-49	-8.615850e-47
Msa0142330	Msa0966270	0.805670	1.193604e-49	-8.615850e-47
Msa0142330	Msa1126510	0.813406	2.601587e-51	-8.615850e-47
Msa0142330	Msa1126770	0.838425	2.897708e-57	-8.615850e-47
Msa0142330	Msa1166860	0.818960	1.492095e-52	-8.615850e-47
Msa0142330	Msa1307950	0.802104	6.572270e-49	-8.615850e-47
Msa0142330	Msa1321790	0.813054	3.108330e-51	-8.615850e-47
Msa0142330	Msa1370860	0.837393	5.338382e-57	-8.615850e-47
Msa0142330	Msa1428520	0.801521	8.658460e-49	-8.615850e-47
Msa0142330	Msa1442850	0.807133	5.864849e-50	-8.615850e-47
Msa0142330	Msa1465080	0.817804	2.726309e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142330	MtrunA17_Chr4g0076801	95.522	67	3	0	4	70	3	69	4.00e-38	120
Msa0142330	MtrunA17_Chr2g0297841	60.345	58	22	1	8	64	3	60	1.34e-18	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142330	AT1G14450.1	71.186	59	16	1	7	64	7	65	2.12e-26	92.0
Msa0142330	AT1G14450.2	71.186	59	16	1	7	64	31	89	3.05e-26	92.4
Msa0142330	AT2G02510.1	62.121	66	23	1	7	70	7	72	1.92e-24	87.0

Find 44 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCATCGCTCTTGTCGCTTT+TGG	0.259915	1_4:-1234811	Msa0142330:CDS
AATCGATCGATCGGAAGAAA+TGG	0.380641	1_4:-1234935	Msa0142330:exon
TCCTCCTCCACCTGACTCAA+TGG	0.393348	1_4:+1234380	None:intergenic
GGAAGAGGAATTCATCTTGT+TGG	0.397407	1_4:+1234762	None:intergenic
TAATAATTTGCTTAGGAGGA+TGG	0.414912	1_4:-1234246	Msa0142330:three_prime_UTR
TACTTTCGCAGGTGAAGGCT+GGG	0.420519	1_4:-1234405	Msa0142330:intron
TAAGCAAATTATTACATTGA+AGG	0.420887	1_4:+1234255	None:intergenic
AATGTAATAATTTGCTTAGG+AGG	0.426038	1_4:-1234250	Msa0142330:three_prime_UTR
TTACTTTCGCAGGTGAAGGC+TGG	0.453299	1_4:-1234406	Msa0142330:intron
TTCAATGTAATAATTTGCTT+AGG	0.471862	1_4:-1234253	Msa0142330:three_prime_UTR
TCTTCAAGAGGAGGGATGAA+TGG	0.473314	1_4:-1234885	Msa0142330:CDS
CGTCATGCCACTCCTGGTCT+CGG	0.476105	1_4:-1234832	Msa0142330:CDS
AAGCAAATTATTACATTGAA+GGG	0.488052	1_4:+1234256	None:intergenic
ATGGTGATGAGAAGAGGAAG+AGG	0.488621	1_4:+1234747	None:intergenic
ACCATTGAGTCAGGTGGAGG+AGG	0.491402	1_4:-1234381	Msa0142330:exon
CAGATGCGTCATGCCACTCC+TGG	0.493267	1_4:-1234838	Msa0142330:CDS
ATCGATCGATCGGAAGAAAT+GGG	0.495092	1_4:-1234934	Msa0142330:exon
ACAAGAGCGATGCCGAGACC+AGG	0.501831	1_4:+1234820	None:intergenic
TCGGAAGAAATGGGAGCAGC+AGG	0.508971	1_4:-1234925	Msa0142330:exon
AGGCTGGGGACCATTGAGTC+AGG	0.523069	1_4:-1234390	Msa0142330:exon
CATTGAAGGGAAAACTAAAG+TGG	0.526551	1_4:+1234269	None:intergenic
TGGAGGAAGCATCCCATGCT+TGG	0.534788	1_4:-1234865	Msa0142330:CDS
AAACAAAACAAGGGAAACAA+TGG	0.546494	1_4:+1234342	None:intergenic
GGGACCATTGAGTCAGGTGG+AGG	0.550031	1_4:-1234384	Msa0142330:exon
CGCATCTGATTACCAAGCAT+GGG	0.552200	1_4:+1234853	None:intergenic
AGATACATGGTGATGAGAAG+AGG	0.553982	1_4:+1234741	None:intergenic
ACGCATCTGATTACCAAGCA+TGG	0.557487	1_4:+1234852	None:intergenic
AAGAAGAAGAAGGAGAGAGA+AGG	0.560634	1_4:-1234970	Msa0142330:five_prime_UTR
AGCAGAGTTCTTCAAGAGGA+GGG	0.574966	1_4:-1234893	Msa0142330:CDS
TTGAGTCAGGTGGAGGAGGA+AGG	0.578227	1_4:-1234377	Msa0142330:exon
CAGCAGAGTTCTTCAAGAGG+AGG	0.588158	1_4:-1234894	Msa0142330:CDS
ACTTTCGCAGGTGAAGGCTG+GGG	0.593895	1_4:-1234404	Msa0142330:intron
TAACAGTCAACAGATGATGA+TGG	0.601312	1_4:+1234128	None:intergenic
ACAGATGATGTGAAATGGAA+TGG	0.602907	1_4:+1234094	None:intergenic
TTATTTAAGCAAATTCATTG+AGG	0.610376	1_4:+1234313	None:intergenic
GAGGTAGAACAAACAAAACA+AGG	0.612383	1_4:+1234332	None:intergenic
TATCAATCGAATCGATCGAT+CGG	0.616208	1_4:-1234944	Msa0142330:five_prime_UTR
AGCGATGCCGAGACCAGGAG+TGG	0.620806	1_4:+1234825	None:intergenic
CTGGGGACCATTGAGTCAGG+TGG	0.631574	1_4:-1234387	Msa0142330:exon
TCAAGAGGAGGGATGAATGG+AGG	0.649135	1_4:-1234882	Msa0142330:CDS
AACAGTGAAAGAAAGTAGTG+AGG	0.665888	1_4:+1234192	None:intergenic
ATACAGCAGAGTTCTTCAAG+AGG	0.667371	1_4:-1234897	Msa0142330:CDS
GGGAAACAATGGATTGACGA+TGG	0.688078	1_4:+1234353	None:intergenic
AGGTAGAACAAACAAAACAA+GGG	0.691412	1_4:+1234333	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGCATTGTTATAATTTAAT+GGG	+	chr1_4:1234618-1234637	None:intergenic	15.0%
!!!	AAGCATTGTTATAATTTAAT+GGG	+	chr1_4:1234618-1234637	None:intergenic	15.0%
!!	TAAGAATAAGAATAGATACA+TGG	+	chr1_4:1234375-1234394	None:intergenic	20.0%
!!	TATTCTTATTCTTACTCTTA+GGG	-	chr1_4:1234381-1234400	Msa0142330:exon	20.0%
!!	ATAAAATGTAAATTGGGAAT+GGG	+	chr1_4:1234454-1234473	None:intergenic	20.0%
!!	AATAAAATGTAAATTGGGAA+TGG	+	chr1_4:1234455-1234474	None:intergenic	20.0%
!!	GCAGTAATAAAATGTAAATT+GGG	+	chr1_4:1234460-1234479	None:intergenic	20.0%
!!	AGCAGTAATAAAATGTAAAT+TGG	+	chr1_4:1234461-1234480	None:intergenic	20.0%
!!!	ATTTACATTTTATTACTGCT+AGG	-	chr1_4:1234461-1234480	Msa0142330:intron	20.0%
!!!	TTTACATTTTATTACTGCTA+GGG	-	chr1_4:1234462-1234481	Msa0142330:intron	20.0%
!!!	GAAGCATTGTTATAATTTAA+TGG	+	chr1_4:1234619-1234638	None:intergenic	20.0%
!!	TTATTTAAGCAAATTCATTG+AGG	+	chr1_4:1234790-1234809	None:intergenic	20.0%
!!	AAGCAAATTATTACATTGAA+GGG	+	chr1_4:1234847-1234866	None:intergenic	20.0%
!!	TAAGCAAATTATTACATTGA+AGG	+	chr1_4:1234848-1234867	None:intergenic	20.0%
!!!	TTCAATGTAATAATTTGCTT+AGG	-	chr1_4:1234847-1234866	Msa0142330:CDS	20.0%
!!	TAAGAATAAGAATAGATACA+TGG	+	chr1_4:1234375-1234394	None:intergenic	20.0%
!!	TATTCTTATTCTTACTCTTA+GGG	-	chr1_4:1234381-1234400	Msa0142330:exon	20.0%
!!	ATAAAATGTAAATTGGGAAT+GGG	+	chr1_4:1234454-1234473	None:intergenic	20.0%
!!	AATAAAATGTAAATTGGGAA+TGG	+	chr1_4:1234455-1234474	None:intergenic	20.0%
!!	GCAGTAATAAAATGTAAATT+GGG	+	chr1_4:1234460-1234479	None:intergenic	20.0%
!!	AGCAGTAATAAAATGTAAAT+TGG	+	chr1_4:1234461-1234480	None:intergenic	20.0%
!!!	ATTTACATTTTATTACTGCT+AGG	-	chr1_4:1234461-1234480	Msa0142330:intron	20.0%
!!!	TTTACATTTTATTACTGCTA+GGG	-	chr1_4:1234462-1234481	Msa0142330:intron	20.0%
!!!	GAAGCATTGTTATAATTTAA+TGG	+	chr1_4:1234619-1234638	None:intergenic	20.0%
!!	TTATTTAAGCAAATTCATTG+AGG	+	chr1_4:1234790-1234809	None:intergenic	20.0%
!!	AAGCAAATTATTACATTGAA+GGG	+	chr1_4:1234847-1234866	None:intergenic	20.0%
!!	TAAGCAAATTATTACATTGA+AGG	+	chr1_4:1234848-1234867	None:intergenic	20.0%
!!!	TTCAATGTAATAATTTGCTT+AGG	-	chr1_4:1234847-1234866	Msa0142330:CDS	20.0%
!	CTATTCTTATTCTTACTCTT+AGG	-	chr1_4:1234380-1234399	Msa0142330:exon	25.0%
!	TGTTTAGAAAGAATAGAGAT+TGG	-	chr1_4:1234558-1234577	Msa0142330:intron	25.0%
!!!	AATGTAATAATTTGCTTAGG+AGG	-	chr1_4:1234850-1234869	Msa0142330:CDS	25.0%
!!!	TTTTTCACTTTTCAGCTTTT+TGG	+	chr1_4:1234109-1234128	None:intergenic	25.0%
!	CTATTCTTATTCTTACTCTT+AGG	-	chr1_4:1234380-1234399	Msa0142330:exon	25.0%
!	TGTTTAGAAAGAATAGAGAT+TGG	-	chr1_4:1234558-1234577	Msa0142330:intron	25.0%
!!!	AATGTAATAATTTGCTTAGG+AGG	-	chr1_4:1234850-1234869	Msa0142330:CDS	25.0%
	AGTGAAAAAGAAGAAGAAGA+AGG	-	chr1_4:1234120-1234139	Msa0142330:three_prime_UTR	30.0%
	CTTAATTACATTACTTTCGC+AGG	-	chr1_4:1234684-1234703	Msa0142330:intron	30.0%
	AAACAAAACAAGGGAAACAA+TGG	+	chr1_4:1234761-1234780	None:intergenic	30.0%
	AGGTAGAACAAACAAAACAA+GGG	+	chr1_4:1234770-1234789	None:intergenic	30.0%
!!	TAATAATTTGCTTAGGAGGA+TGG	-	chr1_4:1234854-1234873	Msa0142330:CDS	30.0%
	AGTGAAAAAGAAGAAGAAGA+AGG	-	chr1_4:1234120-1234139	Msa0142330:three_prime_UTR	30.0%
	CTTAATTACATTACTTTCGC+AGG	-	chr1_4:1234684-1234703	Msa0142330:intron	30.0%
	AAACAAAACAAGGGAAACAA+TGG	+	chr1_4:1234761-1234780	None:intergenic	30.0%
	AGGTAGAACAAACAAAACAA+GGG	+	chr1_4:1234770-1234789	None:intergenic	30.0%
!!	TAATAATTTGCTTAGGAGGA+TGG	-	chr1_4:1234854-1234873	Msa0142330:CDS	30.0%
	TATCAATCGAATCGATCGAT+CGG	-	chr1_4:1234156-1234175	Msa0142330:three_prime_UTR	35.0%
	AATGTAAATTGGGAATGGGA+GGG	+	chr1_4:1234450-1234469	None:intergenic	35.0%
	AAATGTAAATTGGGAATGGG+AGG	+	chr1_4:1234451-1234470	None:intergenic	35.0%
	TTCATTATGAAAACGAGTCC+CGG	+	chr1_4:1234589-1234608	None:intergenic	35.0%
	TGAATAGTACAAACAGACAC+AGG	+	chr1_4:1234658-1234677	None:intergenic	35.0%
	GAGGTAGAACAAACAAAACA+AGG	+	chr1_4:1234771-1234790	None:intergenic	35.0%
	CATTGAAGGGAAAACTAAAG+TGG	+	chr1_4:1234834-1234853	None:intergenic	35.0%
	AACAGTGAAAGAAAGTAGTG+AGG	+	chr1_4:1234911-1234930	None:intergenic	35.0%
	TAACAGTCAACAGATGATGA+TGG	+	chr1_4:1234975-1234994	Msa0142340:five_prime_UTR	35.0%
	TATCAATCGAATCGATCGAT+CGG	-	chr1_4:1234156-1234175	Msa0142330:three_prime_UTR	35.0%
	AATGTAAATTGGGAATGGGA+GGG	+	chr1_4:1234450-1234469	None:intergenic	35.0%
	AAATGTAAATTGGGAATGGG+AGG	+	chr1_4:1234451-1234470	None:intergenic	35.0%
	TTCATTATGAAAACGAGTCC+CGG	+	chr1_4:1234589-1234608	None:intergenic	35.0%
	TGAATAGTACAAACAGACAC+AGG	+	chr1_4:1234658-1234677	None:intergenic	35.0%
	GAGGTAGAACAAACAAAACA+AGG	+	chr1_4:1234771-1234790	None:intergenic	35.0%
	CATTGAAGGGAAAACTAAAG+TGG	+	chr1_4:1234834-1234853	None:intergenic	35.0%
	AACAGTGAAAGAAAGTAGTG+AGG	+	chr1_4:1234911-1234930	None:intergenic	35.0%
	TAACAGTCAACAGATGATGA+TGG	+	chr1_4:1234975-1234994	Msa0142340:five_prime_UTR	35.0%
	AAGAAGAAGAAGGAGAGAGA+AGG	-	chr1_4:1234130-1234149	Msa0142330:three_prime_UTR	40.0%
	AATCGATCGATCGGAAGAAA+TGG	-	chr1_4:1234165-1234184	Msa0142330:three_prime_UTR	40.0%
	ATCGATCGATCGGAAGAAAT+GGG	-	chr1_4:1234166-1234185	Msa0142330:three_prime_UTR	40.0%
	ATACAGCAGAGTTCTTCAAG+AGG	-	chr1_4:1234203-1234222	Msa0142330:three_prime_UTR	40.0%
	GGAAGAGGAATTCATCTTGT+TGG	+	chr1_4:1234341-1234360	None:intergenic	40.0%
!	AGATACATGGTGATGAGAAG+AGG	+	chr1_4:1234362-1234381	None:intergenic	40.0%
!	AGAATAGAGATTGGTTGCAC+CGG	-	chr1_4:1234567-1234586	Msa0142330:intron	40.0%
	TACATTACTTTCGCAGGTGA+AGG	-	chr1_4:1234690-1234709	Msa0142330:intron	40.0%
	AAGAAGAAGAAGGAGAGAGA+AGG	-	chr1_4:1234130-1234149	Msa0142330:three_prime_UTR	40.0%
	AATCGATCGATCGGAAGAAA+TGG	-	chr1_4:1234165-1234184	Msa0142330:three_prime_UTR	40.0%
	ATCGATCGATCGGAAGAAAT+GGG	-	chr1_4:1234166-1234185	Msa0142330:three_prime_UTR	40.0%
	ATACAGCAGAGTTCTTCAAG+AGG	-	chr1_4:1234203-1234222	Msa0142330:three_prime_UTR	40.0%
	GGAAGAGGAATTCATCTTGT+TGG	+	chr1_4:1234341-1234360	None:intergenic	40.0%
!	AGATACATGGTGATGAGAAG+AGG	+	chr1_4:1234362-1234381	None:intergenic	40.0%
!	AGAATAGAGATTGGTTGCAC+CGG	-	chr1_4:1234567-1234586	Msa0142330:intron	40.0%
	TACATTACTTTCGCAGGTGA+AGG	-	chr1_4:1234690-1234709	Msa0142330:intron	40.0%
	AGCAGAGTTCTTCAAGAGGA+GGG	-	chr1_4:1234207-1234226	Msa0142330:three_prime_UTR	45.0%
	TCTTCAAGAGGAGGGATGAA+TGG	-	chr1_4:1234215-1234234	Msa0142330:three_prime_UTR	45.0%
	CGCATCTGATTACCAAGCAT+GGG	+	chr1_4:1234250-1234269	None:intergenic	45.0%
	ACGCATCTGATTACCAAGCA+TGG	+	chr1_4:1234251-1234270	None:intergenic	45.0%
!	ATGGTGATGAGAAGAGGAAG+AGG	+	chr1_4:1234356-1234375	None:intergenic	45.0%
	CATACAACAAGCAACGATGC+TGG	+	chr1_4:1234519-1234538	None:intergenic	45.0%
!	GAATAGAGATTGGTTGCACC+GGG	-	chr1_4:1234568-1234587	Msa0142330:intron	45.0%
!!	GGGAAACAATGGATTGACGA+TGG	+	chr1_4:1234750-1234769	None:intergenic	45.0%
	AGCAGAGTTCTTCAAGAGGA+GGG	-	chr1_4:1234207-1234226	Msa0142330:three_prime_UTR	45.0%
	TCTTCAAGAGGAGGGATGAA+TGG	-	chr1_4:1234215-1234234	Msa0142330:three_prime_UTR	45.0%
	CGCATCTGATTACCAAGCAT+GGG	+	chr1_4:1234250-1234269	None:intergenic	45.0%
	ACGCATCTGATTACCAAGCA+TGG	+	chr1_4:1234251-1234270	None:intergenic	45.0%
!	ATGGTGATGAGAAGAGGAAG+AGG	+	chr1_4:1234356-1234375	None:intergenic	45.0%
	CATACAACAAGCAACGATGC+TGG	+	chr1_4:1234519-1234538	None:intergenic	45.0%
!	GAATAGAGATTGGTTGCACC+GGG	-	chr1_4:1234568-1234587	Msa0142330:intron	45.0%
!!	GGGAAACAATGGATTGACGA+TGG	+	chr1_4:1234750-1234769	None:intergenic	45.0%
	CAGCAGAGTTCTTCAAGAGG+AGG	-	chr1_4:1234206-1234225	Msa0142330:three_prime_UTR	50.0%
	TCAAGAGGAGGGATGAATGG+AGG	-	chr1_4:1234218-1234237	Msa0142330:three_prime_UTR	50.0%
	TTACTTTCGCAGGTGAAGGC+TGG	-	chr1_4:1234694-1234713	Msa0142330:intron	50.0%
	TACTTTCGCAGGTGAAGGCT+GGG	-	chr1_4:1234695-1234714	Msa0142330:intron	50.0%
	CAGCAGAGTTCTTCAAGAGG+AGG	-	chr1_4:1234206-1234225	Msa0142330:three_prime_UTR	50.0%
	TCAAGAGGAGGGATGAATGG+AGG	-	chr1_4:1234218-1234237	Msa0142330:three_prime_UTR	50.0%
	TTACTTTCGCAGGTGAAGGC+TGG	-	chr1_4:1234694-1234713	Msa0142330:intron	50.0%
	TACTTTCGCAGGTGAAGGCT+GGG	-	chr1_4:1234695-1234714	Msa0142330:intron	50.0%
	TCGGAAGAAATGGGAGCAGC+AGG	-	chr1_4:1234175-1234194	Msa0142330:three_prime_UTR	55.0%
!!	TGGAGGAAGCATCCCATGCT+TGG	-	chr1_4:1234235-1234254	Msa0142330:three_prime_UTR	55.0%
!!!	GGCATCGCTCTTGTCGCTTT+TGG	-	chr1_4:1234289-1234308	Msa0142330:three_prime_UTR	55.0%
	ACTTTCGCAGGTGAAGGCTG+GGG	-	chr1_4:1234696-1234715	Msa0142330:intron	55.0%
	ACCATTGAGTCAGGTGGAGG+AGG	-	chr1_4:1234719-1234738	Msa0142330:intron	55.0%
	TCCTCCTCCACCTGACTCAA+TGG	+	chr1_4:1234723-1234742	None:intergenic	55.0%
	TTGAGTCAGGTGGAGGAGGA+AGG	-	chr1_4:1234723-1234742	Msa0142330:intron	55.0%
	TCGGAAGAAATGGGAGCAGC+AGG	-	chr1_4:1234175-1234194	Msa0142330:three_prime_UTR	55.0%
!!	TGGAGGAAGCATCCCATGCT+TGG	-	chr1_4:1234235-1234254	Msa0142330:three_prime_UTR	55.0%
!!!	GGCATCGCTCTTGTCGCTTT+TGG	-	chr1_4:1234289-1234308	Msa0142330:three_prime_UTR	55.0%
	ACTTTCGCAGGTGAAGGCTG+GGG	-	chr1_4:1234696-1234715	Msa0142330:intron	55.0%
	ACCATTGAGTCAGGTGGAGG+AGG	-	chr1_4:1234719-1234738	Msa0142330:intron	55.0%
	TCCTCCTCCACCTGACTCAA+TGG	+	chr1_4:1234723-1234742	None:intergenic	55.0%
	TTGAGTCAGGTGGAGGAGGA+AGG	-	chr1_4:1234723-1234742	Msa0142330:intron	55.0%
	CAGATGCGTCATGCCACTCC+TGG	-	chr1_4:1234262-1234281	Msa0142330:three_prime_UTR	60.0%
	CGTCATGCCACTCCTGGTCT+CGG	-	chr1_4:1234268-1234287	Msa0142330:three_prime_UTR	60.0%
	ACAAGAGCGATGCCGAGACC+AGG	+	chr1_4:1234283-1234302	None:intergenic	60.0%
	AGGCTGGGGACCATTGAGTC+AGG	-	chr1_4:1234710-1234729	Msa0142330:intron	60.0%
	CTGGGGACCATTGAGTCAGG+TGG	-	chr1_4:1234713-1234732	Msa0142330:intron	60.0%
	GGGACCATTGAGTCAGGTGG+AGG	-	chr1_4:1234716-1234735	Msa0142330:intron	60.0%
	CAGATGCGTCATGCCACTCC+TGG	-	chr1_4:1234262-1234281	Msa0142330:three_prime_UTR	60.0%
	CGTCATGCCACTCCTGGTCT+CGG	-	chr1_4:1234268-1234287	Msa0142330:three_prime_UTR	60.0%
	ACAAGAGCGATGCCGAGACC+AGG	+	chr1_4:1234283-1234302	None:intergenic	60.0%
	AGGCTGGGGACCATTGAGTC+AGG	-	chr1_4:1234710-1234729	Msa0142330:intron	60.0%
	CTGGGGACCATTGAGTCAGG+TGG	-	chr1_4:1234713-1234732	Msa0142330:intron	60.0%
	GGGACCATTGAGTCAGGTGG+AGG	-	chr1_4:1234716-1234735	Msa0142330:intron	60.0%
	AGCGATGCCGAGACCAGGAG+TGG	+	chr1_4:1234278-1234297	None:intergenic	65.0%
	AGCGATGCCGAGACCAGGAG+TGG	+	chr1_4:1234278-1234297	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	1234109	1235013	1234109	ID=Msa0142330;Name=Msa0142330
chr1_4	mRNA	1234109	1235013	1234109	ID=Msa0142330-mRNA-1;Parent=Msa0142330;Name=Msa0142330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=75\|1\|1\|1\|1\|1\|2\|287\|70
chr1_4	exon	1234109	1234416	1234109	ID=Msa0142330-mRNA-1:exon:405;Parent=Msa0142330-mRNA-1
chr1_4	exon	1234747	1235013	1234747	ID=Msa0142330-mRNA-1:exon:404;Parent=Msa0142330-mRNA-1
chr1_4	five_prime_UTR	1234939	1235013	1234939	ID=Msa0142330-mRNA-1:five_prime_utr;Parent=Msa0142330-mRNA-1
chr1_4	CDS	1234747	1234938	1234747	ID=Msa0142330-mRNA-1:cds;Parent=Msa0142330-mRNA-1
chr1_4	CDS	1234396	1234416	1234396	ID=Msa0142330-mRNA-1:cds;Parent=Msa0142330-mRNA-1
chr1_4	three_prime_UTR	1234109	1234395	1234109	ID=Msa0142330-mRNA-1:three_prime_utr;Parent=Msa0142330-mRNA-1

Gene Sequence	>Msa0142330 ATGGGAGCAGCAGGAAAGAATACAGCAGAGTTCTTCAAGAGGAGGGATGAATGGAGGAAGCATCCCATGCTTGGTAATCAGATGCGTCATGCCACTCCTGGTCTCGGCATCGCTCTTGTCGCTTTTGGTGTCTATCTTGTTGCTGAACAAGTCTCCAACAAGATGAATTCCTCTTCCTCTTCTCATCACCATGTGAAGGCTGGGGACCATTGA
Protein sequence	>Msa0142330 MGAAGKNTAEFFKRRDEWRKHPMLGNQMRHATPGLGIALVAFGVYLVAEQVSNKMNSSSSSHHHVKAGDH*