AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0566700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566700 MtrunA17_Chr8g0381271 100.000 64 0 0 1 64 1 64 4.47e-42 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566700 AT1G67785.1 77.419 62 13 1 1 62 1 61 2.32e-30 101

Find 61 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATTGTTCCAACAATTAT+TGG 0.198021 4_1:+67843118 None:intergenic
CATGATATTGCTCCCTAAAA+TGG 0.275849 4_1:-67843147 Msa0566700:three_prime_UTR
TTTGTCTGTGTATTGGTATT+TGG 0.276417 4_1:-67843082 Msa0566700:three_prime_UTR
CAAAGGAAAATAAAGTTTCT+TGG 0.284054 4_1:+67843009 None:intergenic
TCATATGCTCAAATAAAATA+TGG 0.321339 4_1:-67843231 Msa0566700:three_prime_UTR
GATACATTTGCCTGCTTGTT+GGG 0.334856 4_1:-67842816 Msa0566700:three_prime_UTR
GGGTCAGAGAACAATTAATT+CGG 0.374447 4_1:-67843353 Msa0566700:CDS
TGCAGAATAAAGATTCAAAA+TGG 0.377318 4_1:-67843843 Msa0566700:exon
ACATTTGTGGGCTATTGATC+TGG 0.386019 4_1:-67842921 Msa0566700:three_prime_UTR
GAATTTAAGTCTACATTTGT+GGG 0.398198 4_1:-67842933 Msa0566700:three_prime_UTR
ATTTCCCTATGTTATATTGA+CGG 0.418191 4_1:-67842717 None:intergenic
GGAATTTAAGTCTACATTTG+TGG 0.424860 4_1:-67842934 Msa0566700:three_prime_UTR
GATGTGGAAGCTGGCTTAGT+AGG 0.428906 4_1:-67842977 Msa0566700:three_prime_UTR
GTGAAGGTTGCAACGTACTT+TGG 0.431142 4_1:-67843821 Msa0566700:CDS
GGATCATGTGTGGAAGTTGC+TGG 0.438211 4_1:-67842955 Msa0566700:three_prime_UTR
CCATGGACAAAGTCCATGTC+TGG 0.443394 4_1:-67843766 Msa0566700:CDS
TGCTCTTCATCAGGACGAAA+AGG 0.451579 4_1:-67843741 Msa0566700:intron
AGATACATTTGCCTGCTTGT+TGG 0.455588 4_1:-67842817 Msa0566700:three_prime_UTR
CACGATAAAGAGATTGAAGA+AGG 0.457718 4_1:+67842753 None:intergenic
TTGATCCGTCAATATAACAT+AGG 0.459663 4_1:+67842712 None:intergenic
ATAACATAGGGAAATTAAGT+AGG 0.460179 4_1:+67842725 None:intergenic
GGTCAGAGAACAATTAATTC+GGG 0.478951 4_1:-67843352 Msa0566700:CDS
TGGTACTTGCCAATAATTGT+TGG 0.479210 4_1:-67843127 Msa0566700:three_prime_UTR
TGTCTGGATTGCTCTTCATC+AGG 0.479924 4_1:-67843750 Msa0566700:CDS
ACTTCTTCGTTGAGATGAGA+TGG 0.481225 4_1:+67843917 None:intergenic
TACTCAAAGGAAATGACAAC+AGG 0.482928 4_1:+67842878 None:intergenic
TGATCCGTCAATATAACATA+GGG 0.490282 4_1:+67842713 None:intergenic
AACGTACTTTGGAATGTCCT+TGG 0.500806 4_1:-67843810 Msa0566700:CDS
AGAAGGAAGCGGAGATCAGA+AGG 0.504547 4_1:-67843374 Msa0566700:CDS
TAACATAGGGAAATTAAGTA+GGG 0.511508 4_1:+67842726 None:intergenic
TGGACTATGTGAAATAATGA+TGG 0.513012 4_1:-67843994 None:intergenic
CCAGACATGGACTTTGTCCA+TGG 0.515687 4_1:+67843766 None:intergenic
GCATTTCAAATTTAATTGAG+TGG 0.516067 4_1:-67843198 Msa0566700:three_prime_UTR
ATGTGGAAGCTGGCTTAGTA+GGG 0.518572 4_1:-67842976 Msa0566700:three_prime_UTR
TCCACATCTCACACTTTCAA+AGG 0.522098 4_1:+67842992 None:intergenic
TGTGTATTGGTATTTGGCGT+GGG 0.526742 4_1:-67843076 Msa0566700:three_prime_UTR
TGTGTTGCAGCAAGAGAGGT+TGG 0.535566 4_1:-67843397 Msa0566700:intron
TTCGTTGAGATGAGATGGGA+AGG 0.540040 4_1:+67843922 None:intergenic
TAATTTGCTGCCCAACAAGC+AGG 0.540779 4_1:+67842806 None:intergenic
AGAGAGGTTGGAGAAGGAAG+CGG 0.542879 4_1:-67843385 Msa0566700:CDS
ATCTCATCTCAACGAAGAAG+TGG 0.550078 4_1:-67843915 Msa0566700:five_prime_UTR
CTGTGTATTGGTATTTGGCG+TGG 0.558588 4_1:-67843077 Msa0566700:three_prime_UTR
AAACTGAATCGAAGCAAAGA+AGG 0.560808 4_1:-67843869 Msa0566700:five_prime_UTR
CCAGAAAACGAATGCTGCCA+AGG 0.569490 4_1:+67843793 None:intergenic
ATATGAAGAATCCTTCTGAC+TGG 0.574630 4_1:+67843323 None:intergenic
CTTCTTCGTTGAGATGAGAT+GGG 0.578259 4_1:+67843918 None:intergenic
AATATCATGTATAACATCAA+AGG 0.580442 4_1:+67843161 None:intergenic
GAAAGCAAACATATACTCAA+AGG 0.581829 4_1:+67842865 None:intergenic
AAAGTGTGAGATGTGGAAGC+TGG 0.595765 4_1:-67842986 Msa0566700:three_prime_UTR
ATAAAGATTCAAAATGGTGA+AGG 0.596896 4_1:-67843837 Msa0566700:exon
ACGATAAAGAGATTGAAGAA+GGG 0.597047 4_1:+67842754 None:intergenic
GCAGCAAGAGAGGTTGGAGA+AGG 0.597767 4_1:-67843391 Msa0566700:intron
GATGAAGAGCAATCCAGACA+TGG 0.613342 4_1:+67843753 None:intergenic
TCGGGAGCAAGCCAGTCAGA+AGG 0.625525 4_1:-67843334 Msa0566700:CDS
CAAGAGAAATTACAAAGCAG+AGG 0.631364 4_1:+67843268 None:intergenic
GAAGGAAGCGGAGATCAGAA+GGG 0.632205 4_1:-67843373 Msa0566700:CDS
TCCTTTGAAAGTGTGAGATG+TGG 0.637352 4_1:-67842993 Msa0566700:three_prime_UTR
GGACTATGTGAAATAATGAT+GGG 0.644512 4_1:-67843993 None:intergenic
GGCTTAGTAGGGATCATGTG+TGG 0.652061 4_1:-67842965 Msa0566700:three_prime_UTR
TCGTTGAGATGAGATGGGAA+GGG 0.656923 4_1:+67843923 None:intergenic
GTAATGTGTTGCAGCAAGAG+AGG 0.669103 4_1:-67843401 Msa0566700:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTGTGTTATTTAAT+TGG - chr4_1:67843274-67843293 Msa0566700:three_prime_UTR 10.0%
!!! AATATTTTGTGTTATTTAAT+TGG - chr4_1:67843274-67843293 Msa0566700:three_prime_UTR 10.0%
!! AGTGAAATTGAAATTAAATA+TGG + chr4_1:67843060-67843079 None:intergenic 15.0%
!! AGTGAAATTGAAATTAAATA+TGG + chr4_1:67843060-67843079 None:intergenic 15.0%
!! ATTAATTTCACTCATTTAAG+AGG - chr4_1:67843097-67843116 Msa0566700:three_prime_UTR 20.0%
!! TCATATGCTCAAATAAAATA+TGG - chr4_1:67843468-67843487 Msa0566700:intron 20.0%
!! AATATCATGTATAACATCAA+AGG + chr4_1:67843541-67843560 None:intergenic 20.0%
!! ATTAATTTCACTCATTTAAG+AGG - chr4_1:67843097-67843116 Msa0566700:three_prime_UTR 20.0%
!! TCATATGCTCAAATAAAATA+TGG - chr4_1:67843468-67843487 Msa0566700:intron 20.0%
!! AATATCATGTATAACATCAA+AGG + chr4_1:67843541-67843560 None:intergenic 20.0%
!! ATTGTGAAAAACACTTTTCT+CGG + chr4_1:67842746-67842765 None:intergenic 25.0%
! TGCAGAATAAAGATTCAAAA+TGG - chr4_1:67842856-67842875 Msa0566700:three_prime_UTR 25.0%
! ATAAAGATTCAAAATGGTGA+AGG - chr4_1:67842862-67842881 Msa0566700:three_prime_UTR 25.0%
! AATATGGAAATTATCCGAAA+AGG + chr4_1:67843044-67843063 None:intergenic 25.0%
! TCGTTATAAGATGAAATCTA+GGG + chr4_1:67843139-67843158 None:intergenic 25.0%
! TTCGTTATAAGATGAAATCT+AGG + chr4_1:67843140-67843159 None:intergenic 25.0%
! GCATTTCAAATTTAATTGAG+TGG - chr4_1:67843501-67843520 Msa0566700:intron 25.0%
! CAAAGGAAAATAAAGTTTCT+TGG + chr4_1:67843693-67843712 None:intergenic 25.0%
! GAATTTAAGTCTACATTTGT+GGG - chr4_1:67843766-67843785 Msa0566700:CDS 25.0%
! TAACATAGGGAAATTAAGTA+GGG + chr4_1:67843976-67843995 None:intergenic 25.0%
! ATAACATAGGGAAATTAAGT+AGG + chr4_1:67843977-67843996 None:intergenic 25.0%
!! ATTGTGAAAAACACTTTTCT+CGG + chr4_1:67842746-67842765 None:intergenic 25.0%
! TGCAGAATAAAGATTCAAAA+TGG - chr4_1:67842856-67842875 Msa0566700:three_prime_UTR 25.0%
! ATAAAGATTCAAAATGGTGA+AGG - chr4_1:67842862-67842881 Msa0566700:three_prime_UTR 25.0%
! AATATGGAAATTATCCGAAA+AGG + chr4_1:67843044-67843063 None:intergenic 25.0%
! TCGTTATAAGATGAAATCTA+GGG + chr4_1:67843139-67843158 None:intergenic 25.0%
! TTCGTTATAAGATGAAATCT+AGG + chr4_1:67843140-67843159 None:intergenic 25.0%
! GCATTTCAAATTTAATTGAG+TGG - chr4_1:67843501-67843520 Msa0566700:intron 25.0%
! CAAAGGAAAATAAAGTTTCT+TGG + chr4_1:67843693-67843712 None:intergenic 25.0%
! GAATTTAAGTCTACATTTGT+GGG - chr4_1:67843766-67843785 Msa0566700:CDS 25.0%
! TAACATAGGGAAATTAAGTA+GGG + chr4_1:67843976-67843995 None:intergenic 25.0%
! ATAACATAGGGAAATTAAGT+AGG + chr4_1:67843977-67843996 None:intergenic 25.0%
! TTGTGAAAAACACTTTTCTC+GGG + chr4_1:67842745-67842764 None:intergenic 30.0%
TGAAGAATTCGAAGAAGTAA+GGG + chr4_1:67842991-67843010 None:intergenic 30.0%
ATGAAGAATTCGAAGAAGTA+AGG + chr4_1:67842992-67843011 None:intergenic 30.0%
!!! CAATTCTTGCTATTCCTTTT+CGG - chr4_1:67843027-67843046 Msa0566700:three_prime_UTR 30.0%
! TGTGGTGAATTTGTTCTTTA+AGG - chr4_1:67843203-67843222 Msa0566700:three_prime_UTR 30.0%
! TATTGGCAAGTACCATTTTA+GGG + chr4_1:67843567-67843586 None:intergenic 30.0%
! TTATTGGCAAGTACCATTTT+AGG + chr4_1:67843568-67843587 None:intergenic 30.0%
AGCATTGTTCCAACAATTAT+TGG + chr4_1:67843584-67843603 None:intergenic 30.0%
! TTTGTCTGTGTATTGGTATT+TGG - chr4_1:67843617-67843636 Msa0566700:intron 30.0%
CCAAAAATAAGCAATGCAAA+CGG + chr4_1:67843667-67843686 None:intergenic 30.0%
GGAATTTAAGTCTACATTTG+TGG - chr4_1:67843765-67843784 Msa0566700:CDS 30.0%
GAAAGCAAACATATACTCAA+AGG + chr4_1:67843837-67843856 None:intergenic 30.0%
ACGATAAAGAGATTGAAGAA+GGG + chr4_1:67843948-67843967 None:intergenic 30.0%
! TTGTGAAAAACACTTTTCTC+GGG + chr4_1:67842745-67842764 None:intergenic 30.0%
TGAAGAATTCGAAGAAGTAA+GGG + chr4_1:67842991-67843010 None:intergenic 30.0%
ATGAAGAATTCGAAGAAGTA+AGG + chr4_1:67842992-67843011 None:intergenic 30.0%
!!! CAATTCTTGCTATTCCTTTT+CGG - chr4_1:67843027-67843046 Msa0566700:three_prime_UTR 30.0%
! TGTGGTGAATTTGTTCTTTA+AGG - chr4_1:67843203-67843222 Msa0566700:three_prime_UTR 30.0%
! TATTGGCAAGTACCATTTTA+GGG + chr4_1:67843567-67843586 None:intergenic 30.0%
! TTATTGGCAAGTACCATTTT+AGG + chr4_1:67843568-67843587 None:intergenic 30.0%
AGCATTGTTCCAACAATTAT+TGG + chr4_1:67843584-67843603 None:intergenic 30.0%
! TTTGTCTGTGTATTGGTATT+TGG - chr4_1:67843617-67843636 Msa0566700:intron 30.0%
CCAAAAATAAGCAATGCAAA+CGG + chr4_1:67843667-67843686 None:intergenic 30.0%
GGAATTTAAGTCTACATTTG+TGG - chr4_1:67843765-67843784 Msa0566700:CDS 30.0%
GAAAGCAAACATATACTCAA+AGG + chr4_1:67843837-67843856 None:intergenic 30.0%
ACGATAAAGAGATTGAAGAA+GGG + chr4_1:67843948-67843967 None:intergenic 30.0%
AAACTGAATCGAAGCAAAGA+AGG - chr4_1:67842830-67842849 Msa0566700:three_prime_UTR 35.0%
GAAGAATTCGAAGAAGTAAG+GGG + chr4_1:67842990-67843009 None:intergenic 35.0%
TACTTATGTGTGCAATGTTG+AGG - chr4_1:67843164-67843183 Msa0566700:three_prime_UTR 35.0%
GGGTCAGAGAACAATTAATT+CGG - chr4_1:67843346-67843365 Msa0566700:CDS 35.0%
GGTCAGAGAACAATTAATTC+GGG - chr4_1:67843347-67843366 Msa0566700:CDS 35.0%
ATATGAAGAATCCTTCTGAC+TGG + chr4_1:67843379-67843398 None:intergenic 35.0%
CAAGAGAAATTACAAAGCAG+AGG + chr4_1:67843434-67843453 None:intergenic 35.0%
CATGATATTGCTCCCTAAAA+TGG - chr4_1:67843552-67843571 Msa0566700:intron 35.0%
TGGTACTTGCCAATAATTGT+TGG - chr4_1:67843572-67843591 Msa0566700:intron 35.0%
!! CCGTTTGCATTGCTTATTTT+TGG - chr4_1:67843664-67843683 Msa0566700:intron 35.0%
TACTCAAAGGAAATGACAAC+AGG + chr4_1:67843824-67843843 None:intergenic 35.0%
CACGATAAAGAGATTGAAGA+AGG + chr4_1:67843949-67843968 None:intergenic 35.0%
AAACTGAATCGAAGCAAAGA+AGG - chr4_1:67842830-67842849 Msa0566700:three_prime_UTR 35.0%
GAAGAATTCGAAGAAGTAAG+GGG + chr4_1:67842990-67843009 None:intergenic 35.0%
TACTTATGTGTGCAATGTTG+AGG - chr4_1:67843164-67843183 Msa0566700:three_prime_UTR 35.0%
GGGTCAGAGAACAATTAATT+CGG - chr4_1:67843346-67843365 Msa0566700:CDS 35.0%
GGTCAGAGAACAATTAATTC+GGG - chr4_1:67843347-67843366 Msa0566700:CDS 35.0%
ATATGAAGAATCCTTCTGAC+TGG + chr4_1:67843379-67843398 None:intergenic 35.0%
CAAGAGAAATTACAAAGCAG+AGG + chr4_1:67843434-67843453 None:intergenic 35.0%
CATGATATTGCTCCCTAAAA+TGG - chr4_1:67843552-67843571 Msa0566700:intron 35.0%
TGGTACTTGCCAATAATTGT+TGG - chr4_1:67843572-67843591 Msa0566700:intron 35.0%
!! CCGTTTGCATTGCTTATTTT+TGG - chr4_1:67843664-67843683 Msa0566700:intron 35.0%
TACTCAAAGGAAATGACAAC+AGG + chr4_1:67843824-67843843 None:intergenic 35.0%
CACGATAAAGAGATTGAAGA+AGG + chr4_1:67843949-67843968 None:intergenic 35.0%
! TTTTCTCGGGCTCAAACTTT+CGG + chr4_1:67842732-67842751 None:intergenic 40.0%
CTTCTTCGTTGAGATGAGAT+GGG + chr4_1:67842784-67842803 None:intergenic 40.0%
ACTTCTTCGTTGAGATGAGA+TGG + chr4_1:67842785-67842804 None:intergenic 40.0%
ATCTCATCTCAACGAAGAAG+TGG - chr4_1:67842784-67842803 Msa0566700:three_prime_UTR 40.0%
!! AACGTACTTTGGAATGTCCT+TGG - chr4_1:67842889-67842908 Msa0566700:three_prime_UTR 40.0%
!! ATTCGTTTTCTGGCAATCCA+TGG - chr4_1:67842916-67842935 Msa0566700:three_prime_UTR 40.0%
! GGTAACTGAGTTATTGACTG+TGG - chr4_1:67843185-67843204 Msa0566700:three_prime_UTR 40.0%
! GCTCGATTTTGTCTGTGTAT+TGG - chr4_1:67843610-67843629 Msa0566700:intron 40.0%
! TGTGTATTGGTATTTGGCGT+GGG - chr4_1:67843623-67843642 Msa0566700:intron 40.0%
! TCCTTTGAAAGTGTGAGATG+TGG - chr4_1:67843706-67843725 Msa0566700:intron 40.0%
TCCACATCTCACACTTTCAA+AGG + chr4_1:67843710-67843729 None:intergenic 40.0%
! ACATTTGTGGGCTATTGATC+TGG - chr4_1:67843778-67843797 Msa0566700:CDS 40.0%
AGATACATTTGCCTGCTTGT+TGG - chr4_1:67843882-67843901 Msa0566700:five_prime_UTR 40.0%
GATACATTTGCCTGCTTGTT+GGG - chr4_1:67843883-67843902 Msa0566700:five_prime_UTR 40.0%
! TTTTCTCGGGCTCAAACTTT+CGG + chr4_1:67842732-67842751 None:intergenic 40.0%
CTTCTTCGTTGAGATGAGAT+GGG + chr4_1:67842784-67842803 None:intergenic 40.0%
ACTTCTTCGTTGAGATGAGA+TGG + chr4_1:67842785-67842804 None:intergenic 40.0%
ATCTCATCTCAACGAAGAAG+TGG - chr4_1:67842784-67842803 Msa0566700:three_prime_UTR 40.0%
!! AACGTACTTTGGAATGTCCT+TGG - chr4_1:67842889-67842908 Msa0566700:three_prime_UTR 40.0%
!! ATTCGTTTTCTGGCAATCCA+TGG - chr4_1:67842916-67842935 Msa0566700:three_prime_UTR 40.0%
! GGTAACTGAGTTATTGACTG+TGG - chr4_1:67843185-67843204 Msa0566700:three_prime_UTR 40.0%
! GCTCGATTTTGTCTGTGTAT+TGG - chr4_1:67843610-67843629 Msa0566700:intron 40.0%
! TGTGTATTGGTATTTGGCGT+GGG - chr4_1:67843623-67843642 Msa0566700:intron 40.0%
! TCCTTTGAAAGTGTGAGATG+TGG - chr4_1:67843706-67843725 Msa0566700:intron 40.0%
TCCACATCTCACACTTTCAA+AGG + chr4_1:67843710-67843729 None:intergenic 40.0%
! ACATTTGTGGGCTATTGATC+TGG - chr4_1:67843778-67843797 Msa0566700:CDS 40.0%
AGATACATTTGCCTGCTTGT+TGG - chr4_1:67843882-67843901 Msa0566700:five_prime_UTR 40.0%
GATACATTTGCCTGCTTGTT+GGG - chr4_1:67843883-67843902 Msa0566700:five_prime_UTR 40.0%
TCGTTGAGATGAGATGGGAA+GGG + chr4_1:67842779-67842798 None:intergenic 45.0%
TTCGTTGAGATGAGATGGGA+AGG + chr4_1:67842780-67842799 None:intergenic 45.0%
GTGAAGGTTGCAACGTACTT+TGG - chr4_1:67842878-67842897 Msa0566700:three_prime_UTR 45.0%
GATGAAGAGCAATCCAGACA+TGG + chr4_1:67842949-67842968 None:intergenic 45.0%
TGTCTGGATTGCTCTTCATC+AGG - chr4_1:67842949-67842968 Msa0566700:three_prime_UTR 45.0%
TGCTCTTCATCAGGACGAAA+AGG - chr4_1:67842958-67842977 Msa0566700:three_prime_UTR 45.0%
GTAATGTGTTGCAGCAAGAG+AGG - chr4_1:67843298-67843317 Msa0566700:three_prime_UTR 45.0%
! CTGTGTATTGGTATTTGGCG+TGG - chr4_1:67843622-67843641 Msa0566700:intron 45.0%
AAAGTGTGAGATGTGGAAGC+TGG - chr4_1:67843713-67843732 Msa0566700:intron 45.0%
! ATGTGGAAGCTGGCTTAGTA+GGG - chr4_1:67843723-67843742 Msa0566700:intron 45.0%
!! TAATTTGCTGCCCAACAAGC+AGG + chr4_1:67843896-67843915 None:intergenic 45.0%
TCGTTGAGATGAGATGGGAA+GGG + chr4_1:67842779-67842798 None:intergenic 45.0%
TTCGTTGAGATGAGATGGGA+AGG + chr4_1:67842780-67842799 None:intergenic 45.0%
GTGAAGGTTGCAACGTACTT+TGG - chr4_1:67842878-67842897 Msa0566700:three_prime_UTR 45.0%
GATGAAGAGCAATCCAGACA+TGG + chr4_1:67842949-67842968 None:intergenic 45.0%
TGTCTGGATTGCTCTTCATC+AGG - chr4_1:67842949-67842968 Msa0566700:three_prime_UTR 45.0%
TGCTCTTCATCAGGACGAAA+AGG - chr4_1:67842958-67842977 Msa0566700:three_prime_UTR 45.0%
GTAATGTGTTGCAGCAAGAG+AGG - chr4_1:67843298-67843317 Msa0566700:three_prime_UTR 45.0%
! CTGTGTATTGGTATTTGGCG+TGG - chr4_1:67843622-67843641 Msa0566700:intron 45.0%
AAAGTGTGAGATGTGGAAGC+TGG - chr4_1:67843713-67843732 Msa0566700:intron 45.0%
! ATGTGGAAGCTGGCTTAGTA+GGG - chr4_1:67843723-67843742 Msa0566700:intron 45.0%
!! TAATTTGCTGCCCAACAAGC+AGG + chr4_1:67843896-67843915 None:intergenic 45.0%
CCAGAAAACGAATGCTGCCA+AGG + chr4_1:67842909-67842928 None:intergenic 50.0%
! CCTTGGCAGCATTCGTTTTC+TGG - chr4_1:67842906-67842925 Msa0566700:three_prime_UTR 50.0%
CCAGACATGGACTTTGTCCA+TGG + chr4_1:67842936-67842955 None:intergenic 50.0%
CCATGGACAAAGTCCATGTC+TGG - chr4_1:67842933-67842952 Msa0566700:three_prime_UTR 50.0%
TGTGTTGCAGCAAGAGAGGT+TGG - chr4_1:67843302-67843321 Msa0566700:exon 50.0%
AGAGAGGTTGGAGAAGGAAG+CGG - chr4_1:67843314-67843333 Msa0566700:exon 50.0%
AGAAGGAAGCGGAGATCAGA+AGG - chr4_1:67843325-67843344 Msa0566700:CDS 50.0%
GAAGGAAGCGGAGATCAGAA+GGG - chr4_1:67843326-67843345 Msa0566700:CDS 50.0%
! GATGTGGAAGCTGGCTTAGT+AGG - chr4_1:67843722-67843741 Msa0566700:intron 50.0%
GGCTTAGTAGGGATCATGTG+TGG - chr4_1:67843734-67843753 Msa0566700:intron 50.0%
GGATCATGTGTGGAAGTTGC+TGG - chr4_1:67843744-67843763 Msa0566700:CDS 50.0%
CCAGAAAACGAATGCTGCCA+AGG + chr4_1:67842909-67842928 None:intergenic 50.0%
! CCTTGGCAGCATTCGTTTTC+TGG - chr4_1:67842906-67842925 Msa0566700:three_prime_UTR 50.0%
CCAGACATGGACTTTGTCCA+TGG + chr4_1:67842936-67842955 None:intergenic 50.0%
CCATGGACAAAGTCCATGTC+TGG - chr4_1:67842933-67842952 Msa0566700:three_prime_UTR 50.0%
TGTGTTGCAGCAAGAGAGGT+TGG - chr4_1:67843302-67843321 Msa0566700:exon 50.0%
AGAGAGGTTGGAGAAGGAAG+CGG - chr4_1:67843314-67843333 Msa0566700:exon 50.0%
AGAAGGAAGCGGAGATCAGA+AGG - chr4_1:67843325-67843344 Msa0566700:CDS 50.0%
GAAGGAAGCGGAGATCAGAA+GGG - chr4_1:67843326-67843345 Msa0566700:CDS 50.0%
! GATGTGGAAGCTGGCTTAGT+AGG - chr4_1:67843722-67843741 Msa0566700:intron 50.0%
GGCTTAGTAGGGATCATGTG+TGG - chr4_1:67843734-67843753 Msa0566700:intron 50.0%
GGATCATGTGTGGAAGTTGC+TGG - chr4_1:67843744-67843763 Msa0566700:CDS 50.0%
GCAGCAAGAGAGGTTGGAGA+AGG - chr4_1:67843308-67843327 Msa0566700:exon 55.0%
GCAGCAAGAGAGGTTGGAGA+AGG - chr4_1:67843308-67843327 Msa0566700:exon 55.0%
TCGGGAGCAAGCCAGTCAGA+AGG - chr4_1:67843365-67843384 Msa0566700:CDS 60.0%
TCGGGAGCAAGCCAGTCAGA+AGG - chr4_1:67843365-67843384 Msa0566700:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 67842719 67844002 67842719 ID=Msa0566700;Name=Msa0566700
chr4_1 mRNA 67842719 67844002 67842719 ID=Msa0566700-mRNA-1;Parent=Msa0566700;Name=Msa0566700-mRNA-1;_AED=0.00;_eAED=0.00;_QI=156|1|1|1|1|1|2|601|64
chr4_1 exon 67842719 67843409 67842719 ID=Msa0566700-mRNA-1:exon:22298;Parent=Msa0566700-mRNA-1
chr4_1 exon 67843742 67844002 67843742 ID=Msa0566700-mRNA-1:exon:22297;Parent=Msa0566700-mRNA-1
chr4_1 five_prime_UTR 67843847 67844002 67843847 ID=Msa0566700-mRNA-1:five_prime_utr;Parent=Msa0566700-mRNA-1
chr4_1 CDS 67843742 67843846 67843742 ID=Msa0566700-mRNA-1:cds;Parent=Msa0566700-mRNA-1
chr4_1 CDS 67843320 67843409 67843320 ID=Msa0566700-mRNA-1:cds;Parent=Msa0566700-mRNA-1
chr4_1 three_prime_UTR 67842719 67843319 67842719 ID=Msa0566700-mRNA-1:three_prime_utr;Parent=Msa0566700-mRNA-1
Gene Sequence

>Msa0566700

ATGGTGAAGGTTGCAACGTACTTTGGAATGTCCTTGGCAGCATTCGTTTTCTGGCAATCCATGGACAAAGTCCATGTCTGGATTGCTCTTCATCAGGACGAAAAGCAAGAGAGGTTGGAGAAGGAAGCGGAGATCAGAAGGGTCAGAGAACAATTAATTCGGGAGCAAGCCAGTCAGAAGGATTCTTCATATTGA
Protein sequence

>Msa0566700

MVKVATYFGMSLAAFVFWQSMDKVHVWIALHQDEKQERLEKEAEIRRVREQLIREQASQKDSSY*