AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0709390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0709390 MtrunA17_Chr7g0265751 98.777 327 4 0 1 327 1 327 0.0 670
Msa0709390 MtrunA17_Chr2g0320801 60.377 318 87 4 1 305 1 292 8.14e-119 345
Msa0709390 MtrunA17_Chr1g0162821 72.362 199 41 2 1 199 1 185 1.26e-106 312
Msa0709390 MtrunA17_Chr2g0276901 37.755 98 58 1 92 186 28 125 5.28e-18 84.7
Msa0709390 MtrunA17_Chr2g0276891 55.102 49 22 0 9 57 184 232 2.88e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0709390 AT5G42820.1 64.026 303 83 5 1 298 1 282 2.79e-115 335
Msa0709390 AT5G42820.2 64.026 303 83 5 1 298 1 282 2.79e-115 335
Msa0709390 AT1G27650.1 65.461 304 74 7 1 292 1 285 5.07e-113 330
Msa0709390 AT1G27650.2 59.843 254 71 7 51 292 1 235 1.92e-73 227
Msa0709390 AT1G10320.2 34.426 183 97 3 9 186 228 392 1.51e-34 134
Msa0709390 AT1G10320.1 34.426 183 97 3 9 186 237 401 1.69e-34 134
Msa0709390 AT3G44785.1 65.753 73 24 1 1 73 1 72 3.90e-29 107

Find 123 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATATTTCATTTCTGTATT+TGG 0.102491 5_1:+542450 Msa0709390:three_prime_UTR
ATGCTCCGCCATCGATTATT+AGG 0.132506 5_1:-539993 None:intergenic
TAAGAGATGTGAAACAATTT+TGG 0.157960 5_1:+542556 Msa0709390:three_prime_UTR
AAGTGTTCTTGGAGTTTATC+AGG 0.235787 5_1:-540256 None:intergenic
TATGAGGGGCGTTCGTATTC+TGG 0.248763 5_1:+541959 Msa0709390:CDS
CATTCAGAAGATCCACAAAA+TGG 0.251545 5_1:-542676 None:intergenic
CTTGAATATTTGTGACAATT+TGG 0.267256 5_1:+540411 Msa0709390:CDS
GATTTGAGGAAGCGATTATT+TGG 0.276417 5_1:+541869 Msa0709390:CDS
TTATCAGGGTCAAGAGATTC+AGG 0.286148 5_1:-540241 None:intergenic
TGCCGAGGACTACGGCTTCT+TGG 0.303158 5_1:-542037 None:intergenic
TACATACATCGAAGTGTTCT+TGG 0.310686 5_1:-540267 None:intergenic
TCGTATTCTGGCCGTGGTTC+TGG 0.312884 5_1:+541971 Msa0709390:CDS
TTACTTGCAGAAATTATTAC+AGG 0.313162 5_1:-542312 None:intergenic
AATAATTTCTGCAAGTAATC+TGG 0.317375 5_1:+542317 Msa0709390:three_prime_UTR
GAGAAGGAAGTTGGAGATTC+TGG 0.338699 5_1:+542172 Msa0709390:CDS
TGAGGTTGTTGAGGTTGATT+AGG 0.344172 5_1:-540199 None:intergenic
TTACAGGGTCTTCTCACTTT+GGG 0.346064 5_1:-542296 None:intergenic
GAGCATTTAGCATCGATATT+CGG 0.354185 5_1:+540010 Msa0709390:CDS
CTTTCTCTTTCTCCTCTGAT+AGG 0.355688 5_1:-542109 None:intergenic
TGAGGTGGTTGAGGAGGTTG+AGG 0.362652 5_1:-540217 None:intergenic
TGCTCCGCCATCGATTATTA+GGG 0.367053 5_1:-539992 None:intergenic
CTTTCTCCTCTGATAGGATT+CGG 0.367164 5_1:-542103 None:intergenic
TGGTTCTGGTAGAAGGGATT+TGG 0.369893 5_1:+541985 Msa0709390:CDS
CCCTCATAGTTACGATTTCT+CGG 0.370767 5_1:-541944 None:intergenic
CTGGCCGTGGTTCTGGTAGA+AGG 0.372740 5_1:+541978 Msa0709390:CDS
GATTCAGGTTGAGGTGGTTG+AGG 0.376296 5_1:-540226 None:intergenic
ATAAAGTTAAGGTTCATATC+AGG 0.384960 5_1:-540166 None:intergenic
TTTGAGGAGCTGAGCAAGTA+TGG 0.394906 5_1:+540376 Msa0709390:CDS
GAGTAGGATTGATAAAGTTA+AGG 0.396234 5_1:-540177 None:intergenic
GGCTTCTTGGCCTTCTACCA+TGG 0.400878 5_1:-542024 None:intergenic
CTAGAATAGAACAATGGAAC+AGG 0.401571 5_1:+542149 Msa0709390:CDS
TGTTGACTTCTCTCCTGTTA+CGG 0.406173 5_1:+541661 Msa0709390:CDS
GCTTCTCGAAAATCCGTAAC+AGG 0.408045 5_1:-541674 None:intergenic
ATTACAGGGTCTTCTCACTT+TGG 0.418323 5_1:-542297 None:intergenic
AAAACTACAGCCAACTCAAT+AGG 0.421667 5_1:-542355 None:intergenic
TGACAATTTGGCTGATCATA+TGG 0.424836 5_1:+540423 Msa0709390:CDS
AGTAGGACTAATGGTGGGTT+TGG 0.429071 5_1:-540116 None:intergenic
AACATAAATAAATCTCGAAA+GGG 0.429853 5_1:+539948 Msa0709390:five_prime_UTR
CTCATGGCTCCCATGGTAGA+AGG 0.441236 5_1:+542014 Msa0709390:CDS
AATCACTATAAAATAGTTAA+CGG 0.444979 5_1:+542624 Msa0709390:three_prime_UTR
TGTTGAGGTTGATTAGGAGT+AGG 0.451883 5_1:-540193 None:intergenic
CCTTTGATTTCAGAGATTTG+AGG 0.452267 5_1:+541855 Msa0709390:intron
GATTATTTGGAAGAAGTAGT+AGG 0.454782 5_1:+541882 Msa0709390:CDS
CCTCATAGTTACGATTTCTC+GGG 0.458427 5_1:-541943 None:intergenic
TGCATCTAAAGAAGATTAGC+AGG 0.461797 5_1:+541753 Msa0709390:CDS
AGATTACCTGGGTGAAATAG+AGG 0.463565 5_1:+542501 Msa0709390:three_prime_UTR
CCCGAGAAATCGTAACTATG+AGG 0.480351 5_1:+541943 Msa0709390:CDS
TCAGGTTGAGGTGGTTGAGG+AGG 0.483616 5_1:-540223 None:intergenic
AAAGAGAAAGTAGTGTAGAA+AGG 0.483749 5_1:+542122 Msa0709390:CDS
TGGAACAGGGAGAAGGAAGT+TGG 0.485919 5_1:+542163 Msa0709390:CDS
ATTCAGAAGATCCACAAAAT+GGG 0.489931 5_1:-542675 None:intergenic
GGAGAGAAGTCAACAATGAT+TGG 0.494146 5_1:-541653 None:intergenic
GGACTCCTGTCTCTTCCCAT+TGG 0.497688 5_1:-542082 None:intergenic
AAACTCTGTTGGAGATTACC+TGG 0.498335 5_1:+542489 Msa0709390:three_prime_UTR
GCTCCGATCTTGAAGTAGAA+TGG 0.500982 5_1:-540058 None:intergenic
CTCATAGTTACGATTTCTCG+GGG 0.504601 5_1:-541942 None:intergenic
CTGGTAGAAGGGATTTGGAC+AGG 0.507028 5_1:+541990 Msa0709390:CDS
GGCCAAGAAGCCGTAGTCCT+CGG 0.507965 5_1:+542035 Msa0709390:CDS
TGAGGAGGTTGAGGTTGTTG+AGG 0.510776 5_1:-540208 None:intergenic
GCTTCTTGGCCTTCTACCAT+GGG 0.511138 5_1:-542023 None:intergenic
GGGTCAAGAGATTCAGGTTG+AGG 0.514083 5_1:-540235 None:intergenic
GGGCGTTCGTATTCTGGCCG+TGG 0.515780 5_1:+541965 Msa0709390:CDS
TTGGAAGAAGTAGTAGGCGT+TGG 0.517691 5_1:+541888 Msa0709390:CDS
GACAGGTCTCATGGCTCCCA+TGG 0.524520 5_1:+542007 Msa0709390:CDS
TTAGTTTGCACCTATTGAGT+TGG 0.528104 5_1:+542345 Msa0709390:three_prime_UTR
GAGAACAAGAGTAGGACTAA+TGG 0.530039 5_1:-540125 None:intergenic
AGGCGGAGTAGAAGTCCAAT+GGG 0.531064 5_1:+542067 Msa0709390:CDS
ATCAGGACGTTGATACATGT+TGG 0.531267 5_1:-540149 None:intergenic
GAAGTCCAATGGGAAGAGAC+AGG 0.531891 5_1:+542077 Msa0709390:CDS
TGTATATGTTCAGTATAAAG+AGG 0.534599 5_1:+540547 Msa0709390:CDS
ACCGTAGTGGAAGCAGAAGC+CGG 0.541010 5_1:+541915 Msa0709390:CDS
CAACATAAATAAATCTCGAA+AGG 0.544906 5_1:+539947 Msa0709390:five_prime_UTR
TGTTCGATGGTAAACTCTGT+TGG 0.545340 5_1:+542478 Msa0709390:three_prime_UTR
TCCGGCTTCTGCTTCCACTA+CGG 0.545385 5_1:-541916 None:intergenic
TGGCCGTGGTTCTGGTAGAA+GGG 0.546507 5_1:+541979 Msa0709390:CDS
CCGAGAAATCGTAACTATGA+GGG 0.550258 5_1:+541944 Msa0709390:CDS
AATAGTTAACGGAAGAAGTG+AGG 0.560146 5_1:+542635 Msa0709390:three_prime_UTR
AGGGATTTGGACAGGTCTCA+TGG 0.560557 5_1:+541998 Msa0709390:CDS
AGTGTTCTTGGAGTTTATCA+GGG 0.562952 5_1:-540255 None:intergenic
AACAATGATTATGAAGAGGC+GGG 0.563713 5_1:+542214 Msa0709390:CDS
TTCCTCTATGCCGAGGACTA+CGG 0.567765 5_1:-542045 None:intergenic
AATGCTCTTATGAATCTCAC+TGG 0.570244 5_1:+540584 Msa0709390:CDS
TGTCCATTCTACTTCAAGAT+CGG 0.570630 5_1:+540055 Msa0709390:CDS
AAGGCGGAGTAGAAGTCCAA+TGG 0.574467 5_1:+542066 Msa0709390:CDS
AGCCGTAGTCCTCGGCATAG+AGG 0.581691 5_1:+542043 Msa0709390:CDS
AGAACAATGGAACAGGGAGA+AGG 0.596602 5_1:+542156 Msa0709390:CDS
ATTTGCAACAGAACAAAGGA+AGG 0.601935 5_1:-542604 None:intergenic
TCATAAGAGCATTAGCAGCA+TGG 0.602037 5_1:-540574 None:intergenic
GTAGTCCTCGGCATAGAGGA+AGG 0.603594 5_1:+542047 Msa0709390:CDS
GAAAGGGGTTCGTGAGAGAG+TGG 0.603769 5_1:+539964 Msa0709390:five_prime_UTR
TAACAATGATTATGAAGAGG+CGG 0.605105 5_1:+542213 Msa0709390:CDS
AGAAGTCAACAATGATTGGT+CGG 0.606403 5_1:-541649 None:intergenic
CATAGTTACGATTTCTCGGG+GGG 0.607441 5_1:-541940 None:intergenic
AGTGATTTGCAACAGAACAA+AGG 0.611402 5_1:-542608 None:intergenic
GATTACCTGGGTGAAATAGA+GGG 0.614308 5_1:+542502 Msa0709390:three_prime_UTR
GGCGAAACCCTAATAATCGA+TGG 0.614459 5_1:+539985 Msa0709390:exon
AACAAGAGTAGGACTAATGG+TGG 0.614682 5_1:-540122 None:intergenic
TCAAGAGATTCAGGTTGAGG+TGG 0.615585 5_1:-540232 None:intergenic
AAGATCGGAGCTTGTAGACA+TGG 0.618937 5_1:+540070 Msa0709390:CDS
CTCCGCCTTCCTCTATGCCG+AGG 0.622022 5_1:-542052 None:intergenic
GTATTTGGATGAATGTTCGA+TGG 0.623654 5_1:+542465 Msa0709390:three_prime_UTR
AGTTAACGGAAGAAGTGAGG+AGG 0.623894 5_1:+542638 Msa0709390:three_prime_UTR
GAGTAACAATGATTATGAAG+AGG 0.626539 5_1:+542210 Msa0709390:CDS
AGTTGGAGATTCTGGTAACA+AGG 0.630652 5_1:+542180 Msa0709390:CDS
AGGAGTCCGAATCCTATCAG+AGG 0.630679 5_1:+542097 Msa0709390:CDS
AACTCTGTTGGAGATTACCT+GGG 0.633510 5_1:+542490 Msa0709390:three_prime_UTR
CGAGAAATCGTAACTATGAG+GGG 0.636005 5_1:+541945 Msa0709390:CDS
GAAGAGAATGTATGCAATCG+AGG 0.636366 5_1:+541713 Msa0709390:CDS
ACATAAATAAATCTCGAAAG+GGG 0.642552 5_1:+539949 Msa0709390:five_prime_UTR
TACTTGCAGAAATTATTACA+GGG 0.643592 5_1:-542311 None:intergenic
ATGTTGGAGAGAACAAGAGT+AGG 0.650220 5_1:-540133 None:intergenic
TCATAGTTACGATTTCTCGG+GGG 0.654148 5_1:-541941 None:intergenic
GGAGAGCTAGAATAGAACAA+TGG 0.656515 5_1:+542143 Msa0709390:CDS
CGGTTCCCTCTATTTCACCC+AGG 0.673223 5_1:-542507 None:intergenic
GCATAGTTGAAATTCAAACA+CGG 0.675622 5_1:-542527 None:intergenic
AGGCGTTGGAATGACCGTAG+TGG 0.684717 5_1:+541902 Msa0709390:CDS
TAGAATAGAACAATGGAACA+GGG 0.687714 5_1:+542150 Msa0709390:CDS
AATCCCTTCTACCAGAACCA+CGG 0.694334 5_1:-541982 None:intergenic
ACAAGAGTAGGACTAATGGT+GGG 0.704223 5_1:-540121 None:intergenic
GAAACCCTAATAATCGATGG+CGG 0.712302 5_1:+539988 Msa0709390:exon
GAGAATGTATGCAATCGAGG+TGG 0.723621 5_1:+541716 Msa0709390:CDS
GTCCTCGGCATAGAGGAAGG+CGG 0.728288 5_1:+542050 Msa0709390:CDS
TGGTATGAAGACGAGAACAG+CGG 0.740225 5_1:-540096 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAAAATAAAAAATTGAA+GGG - chr5_1:540309-540328 None:intergenic 10.0%
!! AAATCAAAATAAAAAATTGA+AGG - chr5_1:540310-540329 None:intergenic 10.0%
!! AACAAAAATCATAAAAATTA+GGG - chr5_1:540510-540529 None:intergenic 10.0%
!! AAACAAAAATCATAAAAATT+AGG - chr5_1:540511-540530 None:intergenic 10.0%
!!! TATGATTTTTGTTTTTTTAT+AGG + chr5_1:540517-540536 Msa0709390:intron 10.0%
!! TATATATATATATGATGATT+TGG + chr5_1:540689-540708 Msa0709390:intron 10.0%
!! ATCAAAATAAAAAATTGAAG+GGG - chr5_1:540308-540327 None:intergenic 15.0%
!!! ATTTTTGTTTTTTTATAGGT+TGG + chr5_1:540521-540540 Msa0709390:intron 15.0%
!! AAAAAGAAAATTACAATTGT+GGG - chr5_1:541394-541413 None:intergenic 15.0%
!! CAAATATTAATGATTATTTC+AGG + chr5_1:541495-541514 Msa0709390:intron 15.0%
!! AATCACTATAAAATAGTTAA+CGG + chr5_1:542624-542643 Msa0709390:three_prime_UTR 15.0%
!! AACATAAATAAATCTCGAAA+GGG + chr5_1:539948-539967 Msa0709390:five_prime_UTR 20.0%
!!! TTTTTATGAAGATCTATTTG+AGG + chr5_1:540360-540379 Msa0709390:CDS 20.0%
!! TTCATAAATCAAAAAGAGAA+AGG - chr5_1:540463-540482 None:intergenic 20.0%
!! TTTAGATTGAGATATTCAAT+TGG + chr5_1:541132-541151 Msa0709390:intron 20.0%
!!! TTTTTTTTTTTTTTCCTTCC+TGG + chr5_1:541366-541385 Msa0709390:intron 20.0%
!! GAAAAAGAAAATTACAATTG+TGG - chr5_1:541395-541414 None:intergenic 20.0%
!!! TATTAATGATTATTTCAGGT+AGG + chr5_1:541499-541518 Msa0709390:intron 20.0%
!!! AAAAATTGAAAAGCATGAAT+AGG - chr5_1:541796-541815 None:intergenic 20.0%
! CAACATAAATAAATCTCGAA+AGG + chr5_1:539947-539966 Msa0709390:five_prime_UTR 25.0%
! ACATAAATAAATCTCGAAAG+GGG + chr5_1:539949-539968 Msa0709390:five_prime_UTR 25.0%
! ATAAAGTTAAGGTTCATATC+AGG - chr5_1:540169-540188 None:intergenic 25.0%
! CTTGAATATTTGTGACAATT+TGG + chr5_1:540411-540430 Msa0709390:CDS 25.0%
! TGTATATGTTCAGTATAAAG+AGG + chr5_1:540547-540566 Msa0709390:CDS 25.0%
!!! TATATATGATGATTTGGTTG+TGG + chr5_1:540695-540714 Msa0709390:intron 25.0%
! TTATTCAATTGTTTGTGCTT+TGG + chr5_1:540731-540750 Msa0709390:intron 25.0%
! ACATGACATATATTGAAACA+TGG - chr5_1:540795-540814 None:intergenic 25.0%
!!! CACTTGATTTATTTTTGTGT+TGG - chr5_1:540864-540883 None:intergenic 25.0%
! ACTAAAGGCAATATTCATAT+TGG + chr5_1:541093-541112 Msa0709390:intron 25.0%
! ATTGAGATATTCAATTGGTT+TGG + chr5_1:541137-541156 Msa0709390:intron 25.0%
!! TTATCTGATGGAATGATTTT+TGG + chr5_1:541235-541254 Msa0709390:intron 25.0%
! TACTTGCAGAAATTATTACA+GGG - chr5_1:542314-542333 None:intergenic 25.0%
! TTACTTGCAGAAATTATTAC+AGG - chr5_1:542315-542334 None:intergenic 25.0%
! AATAATTTCTGCAAGTAATC+TGG + chr5_1:542317-542336 Msa0709390:three_prime_UTR 25.0%
! GGATATTTCATTTCTGTATT+TGG + chr5_1:542450-542469 Msa0709390:three_prime_UTR 25.0%
!! TAAGAGATGTGAAACAATTT+TGG + chr5_1:542556-542575 Msa0709390:three_prime_UTR 25.0%
GAGTAGGATTGATAAAGTTA+AGG - chr5_1:540180-540199 None:intergenic 30.0%
!!! AGGTTCTGAATTTTGTAGTT+AGG + chr5_1:540619-540638 Msa0709390:intron 30.0%
!!! GGTTCTGAATTTTGTAGTTA+GGG + chr5_1:540620-540639 Msa0709390:intron 30.0%
! TAATGAGTTGGATCGAAATT+TGG + chr5_1:540962-540981 Msa0709390:intron 30.0%
TGGTACATGATAGAACATTA+TGG + chr5_1:540982-541001 Msa0709390:intron 30.0%
TGAATCCTTTGTGTAACTAA+AGG + chr5_1:541078-541097 Msa0709390:intron 30.0%
TATTGCCTTTAGTTACACAA+AGG - chr5_1:541086-541105 None:intergenic 30.0%
CAATAAAAGTGGGTACAATA+AGG - chr5_1:541182-541201 None:intergenic 30.0%
!! CTTATTGTACCCACTTTTAT+TGG + chr5_1:541180-541199 Msa0709390:intron 30.0%
!! TGATGGAATGATTTTTGGAT+AGG + chr5_1:541240-541259 Msa0709390:intron 30.0%
!! AATCTCTGAAATCAAAGGTA+AGG - chr5_1:541853-541872 None:intergenic 30.0%
GATTATTTGGAAGAAGTAGT+AGG + chr5_1:541882-541901 Msa0709390:CDS 30.0%
AAAGAGAAAGTAGTGTAGAA+AGG + chr5_1:542122-542141 Msa0709390:CDS 30.0%
TAGAATAGAACAATGGAACA+GGG + chr5_1:542150-542169 Msa0709390:CDS 30.0%
GAGTAACAATGATTATGAAG+AGG + chr5_1:542210-542229 Msa0709390:CDS 30.0%
TAACAATGATTATGAAGAGG+CGG + chr5_1:542213-542232 Msa0709390:CDS 30.0%
GCATAGTTGAAATTCAAACA+CGG - chr5_1:542530-542549 None:intergenic 30.0%
! AACAATTTTGGATCGAATCA+TGG + chr5_1:542568-542587 Msa0709390:three_prime_UTR 30.0%
!! GAGCATTTAGCATCGATATT+CGG + chr5_1:540010-540029 Msa0709390:CDS 35.0%
TGTCCATTCTACTTCAAGAT+CGG + chr5_1:540055-540074 Msa0709390:CDS 35.0%
AGTGTTCTTGGAGTTTATCA+GGG - chr5_1:540258-540277 None:intergenic 35.0%
AAGTGTTCTTGGAGTTTATC+AGG - chr5_1:540259-540278 None:intergenic 35.0%
! TACATACATCGAAGTGTTCT+TGG - chr5_1:540270-540289 None:intergenic 35.0%
TGACAATTTGGCTGATCATA+TGG + chr5_1:540423-540442 Msa0709390:CDS 35.0%
AATGCTCTTATGAATCTCAC+TGG + chr5_1:540584-540603 Msa0709390:CDS 35.0%
! CTCACTGGAAGATTTTATTC+AGG + chr5_1:540599-540618 Msa0709390:CDS 35.0%
TATATACGTTGAAACACGAG+TGG - chr5_1:540831-540850 None:intergenic 35.0%
AGACTAGAGAGGATATGATT+AGG + chr5_1:540921-540940 Msa0709390:intron 35.0%
TAAGAAGGGCCAATAAAAGT+GGG - chr5_1:541192-541211 None:intergenic 35.0%
ACTCCCAAAATGAGTAAGAA+GGG - chr5_1:541206-541225 None:intergenic 35.0%
ATTCCATCAGATAAAGCTCA+AGG - chr5_1:541229-541248 None:intergenic 35.0%
AAATTACAATTGTGGGTACC+AGG - chr5_1:541387-541406 None:intergenic 35.0%
AAGCAATGATAGACAATGCA+CGG - chr5_1:541575-541594 None:intergenic 35.0%
TTGCTACTGACATTGTGATT+TGG + chr5_1:541610-541629 Msa0709390:intron 35.0%
AGAAGTCAACAATGATTGGT+CGG - chr5_1:541652-541671 None:intergenic 35.0%
!! ATTTTCGAGAAGCTACTTGT+AGG + chr5_1:541684-541703 Msa0709390:CDS 35.0%
TGCATCTAAAGAAGATTAGC+AGG + chr5_1:541753-541772 Msa0709390:CDS 35.0%
! AAAAGCATGAATAGGCTATG+TGG - chr5_1:541788-541807 None:intergenic 35.0%
!! CCTCAAATCTCTGAAATCAA+AGG - chr5_1:541858-541877 None:intergenic 35.0%
! CCTTTGATTTCAGAGATTTG+AGG + chr5_1:541855-541874 Msa0709390:intron 35.0%
GATTTGAGGAAGCGATTATT+TGG + chr5_1:541869-541888 Msa0709390:CDS 35.0%
CTAGAATAGAACAATGGAAC+AGG + chr5_1:542149-542168 Msa0709390:CDS 35.0%
AACAATGATTATGAAGAGGC+GGG + chr5_1:542214-542233 Msa0709390:CDS 35.0%
!! TTAGTTTGCACCTATTGAGT+TGG + chr5_1:542345-542364 Msa0709390:three_prime_UTR 35.0%
AAAACTACAGCCAACTCAAT+AGG - chr5_1:542358-542377 None:intergenic 35.0%
!! GTTTTGATATAGTTCAGCAG+TGG + chr5_1:542429-542448 Msa0709390:three_prime_UTR 35.0%
GTATTTGGATGAATGTTCGA+TGG + chr5_1:542465-542484 Msa0709390:three_prime_UTR 35.0%
ATTTGCAACAGAACAAAGGA+AGG - chr5_1:542607-542626 None:intergenic 35.0%
! AGTGATTTGCAACAGAACAA+AGG - chr5_1:542611-542630 None:intergenic 35.0%
AATAGTTAACGGAAGAAGTG+AGG + chr5_1:542635-542654 Msa0709390:three_prime_UTR 35.0%
! AGAAAATCTGACCCATTTTG+TGG + chr5_1:542664-542683 Msa0709390:three_prime_UTR 35.0%
GAAACCCTAATAATCGATGG+CGG + chr5_1:539988-540007 Msa0709390:exon 40.0%
ACAAGAGTAGGACTAATGGT+GGG - chr5_1:540124-540143 None:intergenic 40.0%
AACAAGAGTAGGACTAATGG+TGG - chr5_1:540125-540144 None:intergenic 40.0%
GAGAACAAGAGTAGGACTAA+TGG - chr5_1:540128-540147 None:intergenic 40.0%
ATGTTGGAGAGAACAAGAGT+AGG - chr5_1:540136-540155 None:intergenic 40.0%
ATCAGGACGTTGATACATGT+TGG - chr5_1:540152-540171 None:intergenic 40.0%
! TGTTGAGGTTGATTAGGAGT+AGG - chr5_1:540196-540215 None:intergenic 40.0%
! TGAGGTTGTTGAGGTTGATT+AGG - chr5_1:540202-540221 None:intergenic 40.0%
TTATCAGGGTCAAGAGATTC+AGG - chr5_1:540244-540263 None:intergenic 40.0%
!! TCATAAGAGCATTAGCAGCA+TGG - chr5_1:540577-540596 None:intergenic 40.0%
! GATGCGTGATCTGATAATGA+TGG + chr5_1:540767-540786 Msa0709390:intron 40.0%
AATCAAGTGTAGCAAAGACG+AGG + chr5_1:540875-540894 Msa0709390:intron 40.0%
ATCAAGTGTAGCAAAGACGA+GGG + chr5_1:540876-540895 Msa0709390:intron 40.0%
GGGTGTTAGCATTTGATGTA+TGG + chr5_1:540896-540915 Msa0709390:intron 40.0%
GATGTATGGTAAGACTAGAG+AGG + chr5_1:540910-540929 Msa0709390:intron 40.0%
AGATCCAGAGACTAATGAGT+TGG + chr5_1:540950-540969 Msa0709390:intron 40.0%
!! ACTAAATGGGGTTGGCTAAA+TGG - chr5_1:541019-541038 None:intergenic 40.0%
ATTTAGCCAACCCCATTTAG+TGG + chr5_1:541018-541037 Msa0709390:intron 40.0%
TTTAGCCAACCCCATTTAGT+GGG + chr5_1:541019-541038 Msa0709390:intron 40.0%
AAACCTTACCCCACTAAATG+GGG - chr5_1:541031-541050 None:intergenic 40.0%
CAAACCTTACCCCACTAAAT+GGG - chr5_1:541032-541051 None:intergenic 40.0%
GTAAGAAGGGCCAATAAAAG+TGG - chr5_1:541193-541212 None:intergenic 40.0%
! TTGGCCCTTCTTACTCATTT+TGG + chr5_1:541199-541218 Msa0709390:intron 40.0%
! TGGCCCTTCTTACTCATTTT+GGG + chr5_1:541200-541219 Msa0709390:intron 40.0%
GACTCCCAAAATGAGTAAGA+AGG - chr5_1:541207-541226 None:intergenic 40.0%
! AGTCCTTGAGCTTTATCTGA+TGG + chr5_1:541223-541242 Msa0709390:intron 40.0%
ACAAGCAGTAAGGACAACAT+TGG - chr5_1:541325-541344 None:intergenic 40.0%
TGCAAACAACACAAGCAGTA+AGG - chr5_1:541335-541354 None:intergenic 40.0%
CTACCGAGTTCAGTATTGTT+TGG + chr5_1:541432-541451 Msa0709390:intron 40.0%
CAGCCAAACAATACTGAACT+CGG - chr5_1:541438-541457 None:intergenic 40.0%
AGTATTGTTTGGCTGAGATG+AGG + chr5_1:541443-541462 Msa0709390:intron 40.0%
GATTTGGCTGTCATTCTTCT+AGG + chr5_1:541626-541645 Msa0709390:intron 40.0%
GGAGAGAAGTCAACAATGAT+TGG - chr5_1:541656-541675 None:intergenic 40.0%
!! TGTTGACTTCTCTCCTGTTA+CGG + chr5_1:541661-541680 Msa0709390:CDS 40.0%
GAAGAGAATGTATGCAATCG+AGG + chr5_1:541713-541732 Msa0709390:CDS 40.0%
TCATAGTTACGATTTCTCGG+GGG - chr5_1:541944-541963 None:intergenic 40.0%
CTCATAGTTACGATTTCTCG+GGG - chr5_1:541945-541964 None:intergenic 40.0%
CCTCATAGTTACGATTTCTC+GGG - chr5_1:541946-541965 None:intergenic 40.0%
CCCTCATAGTTACGATTTCT+CGG - chr5_1:541947-541966 None:intergenic 40.0%
CCGAGAAATCGTAACTATGA+GGG + chr5_1:541944-541963 Msa0709390:CDS 40.0%
CGAGAAATCGTAACTATGAG+GGG + chr5_1:541945-541964 Msa0709390:CDS 40.0%
CTTTCTCCTCTGATAGGATT+CGG - chr5_1:542106-542125 None:intergenic 40.0%
CTTTCTCTTTCTCCTCTGAT+AGG - chr5_1:542112-542131 None:intergenic 40.0%
GGAGAGCTAGAATAGAACAA+TGG + chr5_1:542143-542162 Msa0709390:CDS 40.0%
AGTTGGAGATTCTGGTAACA+AGG + chr5_1:542180-542199 Msa0709390:CDS 40.0%
TTACAGGGTCTTCTCACTTT+GGG - chr5_1:542299-542318 None:intergenic 40.0%
ATTACAGGGTCTTCTCACTT+TGG - chr5_1:542300-542319 None:intergenic 40.0%
TGTTCGATGGTAAACTCTGT+TGG + chr5_1:542478-542497 Msa0709390:three_prime_UTR 40.0%
AAACTCTGTTGGAGATTACC+TGG + chr5_1:542489-542508 Msa0709390:three_prime_UTR 40.0%
AACTCTGTTGGAGATTACCT+GGG + chr5_1:542490-542509 Msa0709390:three_prime_UTR 40.0%
AGATTACCTGGGTGAAATAG+AGG + chr5_1:542501-542520 Msa0709390:three_prime_UTR 40.0%
GATTACCTGGGTGAAATAGA+GGG + chr5_1:542502-542521 Msa0709390:three_prime_UTR 40.0%
GGCGAAACCCTAATAATCGA+TGG + chr5_1:539985-540004 Msa0709390:exon 45.0%
! TGCTCCGCCATCGATTATTA+GGG - chr5_1:539995-540014 None:intergenic 45.0%
! ATGCTCCGCCATCGATTATT+AGG - chr5_1:539996-540015 None:intergenic 45.0%
GCTCCGATCTTGAAGTAGAA+TGG - chr5_1:540061-540080 None:intergenic 45.0%
AAGATCGGAGCTTGTAGACA+TGG + chr5_1:540070-540089 Msa0709390:CDS 45.0%
TGGTATGAAGACGAGAACAG+CGG - chr5_1:540099-540118 None:intergenic 45.0%
!! AGTAGGACTAATGGTGGGTT+TGG - chr5_1:540119-540138 None:intergenic 45.0%
!! TCAAGAGATTCAGGTTGAGG+TGG - chr5_1:540235-540254 None:intergenic 45.0%
TTTGAGGAGCTGAGCAAGTA+TGG + chr5_1:540376-540395 Msa0709390:CDS 45.0%
! CGATCCAACTCATTAGTCTC+TGG - chr5_1:540957-540976 None:intergenic 45.0%
TTAGCCAACCCCATTTAGTG+GGG + chr5_1:541020-541039 Msa0709390:intron 45.0%
CCAAACCTTACCCCACTAAA+TGG - chr5_1:541033-541052 None:intergenic 45.0%
CCATTTAGTGGGGTAAGGTT+TGG + chr5_1:541030-541049 Msa0709390:intron 45.0%
TTGGACAGTAGCAGATGTAC+TGG - chr5_1:541306-541325 None:intergenic 45.0%
TACAATTGTGGGTACCAGGA+AGG - chr5_1:541383-541402 None:intergenic 45.0%
AATGTCAGTAGCAATGAGCG+AGG - chr5_1:541604-541623 None:intergenic 45.0%
GCTTCTCGAAAATCCGTAAC+AGG - chr5_1:541677-541696 None:intergenic 45.0%
GAGAATGTATGCAATCGAGG+TGG + chr5_1:541716-541735 Msa0709390:CDS 45.0%
TTGGAAGAAGTAGTAGGCGT+TGG + chr5_1:541888-541907 Msa0709390:CDS 45.0%
CATAGTTACGATTTCTCGGG+GGG - chr5_1:541943-541962 None:intergenic 45.0%
CCCGAGAAATCGTAACTATG+AGG + chr5_1:541943-541962 Msa0709390:CDS 45.0%
AATCCCTTCTACCAGAACCA+CGG - chr5_1:541985-542004 None:intergenic 45.0%
!! TGGTTCTGGTAGAAGGGATT+TGG + chr5_1:541985-542004 Msa0709390:CDS 45.0%
AGAACAATGGAACAGGGAGA+AGG + chr5_1:542156-542175 Msa0709390:CDS 45.0%
GAGAAGGAAGTTGGAGATTC+TGG + chr5_1:542172-542191 Msa0709390:CDS 45.0%
!!! CAGACTCGCTGCCATTTTTT+AGG - chr5_1:542240-542259 None:intergenic 45.0%
CTAAAAAATGGCAGCGAGTC+TGG + chr5_1:542238-542257 Msa0709390:CDS 45.0%
AGTTAACGGAAGAAGTGAGG+AGG + chr5_1:542638-542657 Msa0709390:three_prime_UTR 45.0%
!!! ATTTTTGATTTTTTTTTTTT+AGG + chr5_1:540336-540355 Msa0709390:intron 5.0%
TGAGGAGGTTGAGGTTGTTG+AGG - chr5_1:540211-540230 None:intergenic 50.0%
!! GATTCAGGTTGAGGTGGTTG+AGG - chr5_1:540229-540248 None:intergenic 50.0%
!! GGGTCAAGAGATTCAGGTTG+AGG - chr5_1:540238-540257 None:intergenic 50.0%
CTTACCCCACTAAATGGGGT+TGG - chr5_1:541027-541046 None:intergenic 50.0%
CAACCCCATTTAGTGGGGTA+AGG + chr5_1:541025-541044 Msa0709390:intron 50.0%
ACCTTGTGCTCTGTCACACA+TGG + chr5_1:541264-541283 Msa0709390:intron 50.0%
ACCATGTGTGACAGAGCACA+AGG - chr5_1:541268-541287 None:intergenic 50.0%
!! TATGAGGGGCGTTCGTATTC+TGG + chr5_1:541959-541978 Msa0709390:CDS 50.0%
CTGGTAGAAGGGATTTGGAC+AGG + chr5_1:541990-542009 Msa0709390:CDS 50.0%
AGGGATTTGGACAGGTCTCA+TGG + chr5_1:541998-542017 Msa0709390:CDS 50.0%
! GCTTCTTGGCCTTCTACCAT+GGG - chr5_1:542026-542045 None:intergenic 50.0%
TTCCTCTATGCCGAGGACTA+CGG - chr5_1:542048-542067 None:intergenic 50.0%
AAGGCGGAGTAGAAGTCCAA+TGG + chr5_1:542066-542085 Msa0709390:CDS 50.0%
AGGCGGAGTAGAAGTCCAAT+GGG + chr5_1:542067-542086 Msa0709390:CDS 50.0%
GAAGTCCAATGGGAAGAGAC+AGG + chr5_1:542077-542096 Msa0709390:CDS 50.0%
AGGAGTCCGAATCCTATCAG+AGG + chr5_1:542097-542116 Msa0709390:CDS 50.0%
TGGAACAGGGAGAAGGAAGT+TGG + chr5_1:542163-542182 Msa0709390:CDS 50.0%
GAAGAGGCGGGCCTAAAAAA+TGG + chr5_1:542226-542245 Msa0709390:CDS 50.0%
GAAAGGGGTTCGTGAGAGAG+TGG + chr5_1:539964-539983 Msa0709390:five_prime_UTR 55.0%
TGAGGTGGTTGAGGAGGTTG+AGG - chr5_1:540220-540239 None:intergenic 55.0%
!! TCAGGTTGAGGTGGTTGAGG+AGG - chr5_1:540226-540245 None:intergenic 55.0%
!! AGGCGTTGGAATGACCGTAG+TGG + chr5_1:541902-541921 Msa0709390:CDS 55.0%
! ACCGTAGTGGAAGCAGAAGC+CGG + chr5_1:541915-541934 Msa0709390:CDS 55.0%
TCCGGCTTCTGCTTCCACTA+CGG - chr5_1:541919-541938 None:intergenic 55.0%
TCGTATTCTGGCCGTGGTTC+TGG + chr5_1:541971-541990 Msa0709390:CDS 55.0%
!! TGGCCGTGGTTCTGGTAGAA+GGG + chr5_1:541979-541998 Msa0709390:CDS 55.0%
CTCATGGCTCCCATGGTAGA+AGG + chr5_1:542014-542033 Msa0709390:CDS 55.0%
! GGCTTCTTGGCCTTCTACCA+TGG - chr5_1:542027-542046 None:intergenic 55.0%
GTAGTCCTCGGCATAGAGGA+AGG + chr5_1:542047-542066 Msa0709390:CDS 55.0%
GGACTCCTGTCTCTTCCCAT+TGG - chr5_1:542085-542104 None:intergenic 55.0%
CGGTTCCCTCTATTTCACCC+AGG - chr5_1:542510-542529 None:intergenic 55.0%
TACGATTTCTCGGGGGGCTC+CGG - chr5_1:541937-541956 None:intergenic 60.0%
!! CTGGCCGTGGTTCTGGTAGA+AGG + chr5_1:541978-541997 Msa0709390:CDS 60.0%
GACAGGTCTCATGGCTCCCA+TGG + chr5_1:542007-542026 Msa0709390:CDS 60.0%
GGCCAAGAAGCCGTAGTCCT+CGG + chr5_1:542035-542054 Msa0709390:CDS 60.0%
TGCCGAGGACTACGGCTTCT+TGG - chr5_1:542040-542059 None:intergenic 60.0%
AGCCGTAGTCCTCGGCATAG+AGG + chr5_1:542043-542062 Msa0709390:CDS 60.0%
GTCCTCGGCATAGAGGAAGG+CGG + chr5_1:542050-542069 Msa0709390:CDS 60.0%
GGGCGTTCGTATTCTGGCCG+TGG + chr5_1:541965-541984 Msa0709390:CDS 65.0%
CTCCGCCTTCCTCTATGCCG+AGG - chr5_1:542055-542074 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 539917 542695 539917 ID=Msa0709390;Name=Msa0709390
chr5_1 mRNA 539917 542695 539917 ID=Msa0709390-mRNA-1;Parent=Msa0709390;Name=Msa0709390-mRNA-1;_AED=0.18;_eAED=0.18;_QI=87|1|1|1|1|1|5|419|327
chr5_1 exon 539917 540282 539917 ID=Msa0709390-mRNA-1:exon:199;Parent=Msa0709390-mRNA-1
chr5_1 exon 540358 540444 540358 ID=Msa0709390-mRNA-1:exon:200;Parent=Msa0709390-mRNA-1
chr5_1 exon 540539 540620 540539 ID=Msa0709390-mRNA-1:exon:201;Parent=Msa0709390-mRNA-1
chr5_1 exon 541648 541774 541648 ID=Msa0709390-mRNA-1:exon:202;Parent=Msa0709390-mRNA-1
chr5_1 exon 541868 542695 541868 ID=Msa0709390-mRNA-1:exon:203;Parent=Msa0709390-mRNA-1
chr5_1 five_prime_UTR 539917 540003 539917 ID=Msa0709390-mRNA-1:five_prime_utr;Parent=Msa0709390-mRNA-1
chr5_1 CDS 540004 540282 540004 ID=Msa0709390-mRNA-1:cds;Parent=Msa0709390-mRNA-1
chr5_1 CDS 540358 540444 540358 ID=Msa0709390-mRNA-1:cds;Parent=Msa0709390-mRNA-1
chr5_1 CDS 540539 540620 540539 ID=Msa0709390-mRNA-1:cds;Parent=Msa0709390-mRNA-1
chr5_1 CDS 541648 541774 541648 ID=Msa0709390-mRNA-1:cds;Parent=Msa0709390-mRNA-1
chr5_1 CDS 541868 542276 541868 ID=Msa0709390-mRNA-1:cds;Parent=Msa0709390-mRNA-1
chr5_1 three_prime_UTR 542277 542695 542277 ID=Msa0709390-mRNA-1:three_prime_utr;Parent=Msa0709390-mRNA-1
Gene Sequence

>Msa0709390

ATGGCGGAGCATTTAGCATCGATATTCGGAACAGAGAAAGACAGAGTTAACTGTCCATTCTACTTCAAGATCGGAGCTTGTAGACATGGTGACCGCTGTTCTCGTCTTCATACCAAACCCACCATTAGTCCTACTCTTGTTCTCTCCAACATGTATCAACGTCCTGATATGAACCTTAACTTTATCAATCCTACTCCTAATCAACCTCAACAACCTCAACCTCCTCAACCACCTCAACCTGAATCTCTTGACCCTGATAAACTCCAAGAACACTTCGATGATTTTTATGAAGATCTATTTGAGGAGCTGAGCAAGTATGGTCAAATTCAGAGCTTGAATATTTGTGACAATTTGGCTGATCATATGGTTGGCAATGTATATGTTCAGTATAAAGAGGAAGACCATGCTGCTAATGCTCTTATGAATCTCACTGGAAGATTTTATTCAGGCCGACCAATCATTGTTGACTTCTCTCCTGTTACGGATTTTCGAGAAGCTACTTGTAGGCAGTACGAAGAGAATGTATGCAATCGAGGTGGCTACTGCAACTTTATGCATCTAAAGAAGATTAGCAGAGATTTGAGGAAGCGATTATTTGGAAGAAGTAGTAGGCGTTGGAATGACCGTAGTGGAAGCAGAAGCCGGAGCCCCCCGAGAAATCGTAACTATGAGGGGCGTTCGTATTCTGGCCGTGGTTCTGGTAGAAGGGATTTGGACAGGTCTCATGGCTCCCATGGTAGAAGGCCAAGAAGCCGTAGTCCTCGGCATAGAGGAAGGCGGAGTAGAAGTCCAATGGGAAGAGACAGGAGTCCGAATCCTATCAGAGGAGAAAGAGAAAGTAGTGTAGAAAGGAGAGCTAGAATAGAACAATGGAACAGGGAGAAGGAAGTTGGAGATTCTGGTAACAAGGACAATAAGAGTAACAATGATTATGAAGAGGCGGGCCTAAAAAATGGCAGCGAGTCTGGCAATCATCAGACATAG
Protein sequence

>Msa0709390

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPTISPTLVLSNMYQRPDMNLNFINPTPNQPQQPQPPQPPQPESLDPDKLQEHFDDFYEDLFEELSKYGQIQSLNICDNLADHMVGNVYVQYKEEDHAANALMNLTGRFYSGRPIIVDFSPVTDFREATCRQYEENVCNRGGYCNFMHLKKISRDLRKRLFGRSSRRWNDRSGSRSRSPPRNRNYEGRSYSGRGSGRRDLDRSHGSHGRRPRSRSPRHRGRRSRSPMGRDRSPNPIRGERESSVERRARIEQWNREKEVGDSGNKDNKSNNDYEEAGLKNGSESGNHQT*