AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0725640


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725640 MtrunA17_Chr3g0113701 57.692 52 22 0 4 55 968 1019 5.90e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725640 AT3G11130.1 57.692 52 22 0 4 55 967 1018 2.54e-11 59.3

Find 24 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATTTCACTGCCTTCTT+TGG 0.143713 5_1:+25365994 None:intergenic
GATTTGAATGTAATGTATTT+TGG 0.199982 5_1:-25366061 Msa0725640:CDS
TTGATGAATCAACCGTCTTC+TGG 0.291397 5_1:+25366999 None:intergenic
GCTCAGGGCTCTTGCTTTCT+CGG 0.309822 5_1:+25366959 None:intergenic
GTTCCTCATCGTTCAATTTG+CGG 0.381341 5_1:+25365962 None:intergenic
ATTTATGTGATTTACCATTT+GGG 0.401363 5_1:+25366774 None:intergenic
GGCTGAACTTCCCCATGCCA+TGG 0.485955 5_1:-25366905 Msa0725640:CDS
CAAAGAAGGCAGTGAAATGA+AGG 0.488888 5_1:-25365993 Msa0725640:CDS
TCAAAATATTCAGCTTTCCA+TGG 0.490614 5_1:-25366801 Msa0725640:CDS
TCTCAATCAACCCATGGCAT+GGG 0.494707 5_1:+25366894 None:intergenic
CTGGACTCATAATCGTAAAG+TGG 0.500636 5_1:+25367018 None:intergenic
GTCTCAATCAACCCATGGCA+TGG 0.509828 5_1:+25366893 None:intergenic
TTTACCATTTGGGTCATCCA+TGG 0.522483 5_1:+25366784 None:intergenic
CTTTCCATGGATGACCCAAA+TGG 0.542785 5_1:-25366788 Msa0725640:intron
AAGGCAGTGAAATGAAGGAA+AGG 0.545158 5_1:-25365988 Msa0725640:CDS
GCTGAACTTCCCCATGCCAT+GGG 0.559299 5_1:-25366904 Msa0725640:intron
GAACAAGGAATCTCCAAAGA+AGG 0.561017 5_1:-25366007 Msa0725640:CDS
TGGACTCATAATCGTAAAGT+GGG 0.594969 5_1:+25367019 None:intergenic
CTTGCTTTCTCGGAAAACAG+TGG 0.609184 5_1:+25366969 None:intergenic
AATCATAACATGCATGAACA+AGG 0.612137 5_1:-25366022 Msa0725640:CDS
CTCAATCAACCCATGGCATG+GGG 0.619541 5_1:+25366895 None:intergenic
ACGATTATGAGTCCAGAAGA+CGG 0.640298 5_1:-25367011 Msa0725640:CDS
AAACCGCAAATTGAACGATG+AGG 0.696659 5_1:-25365965 None:intergenic
AAAGTGGGATCTTCTCGCAG+AGG 0.701756 5_1:+25367034 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAAATACTGAAAATT+TGG - chr5_1:25366330-25366349 Msa0725640:intron 15.0%
!!! TAATACAATCTAACTTATTT+TGG - chr5_1:25366462-25366481 Msa0725640:intron 15.0%
!! AACAAAAAATACTGAAAATT+TGG - chr5_1:25366330-25366349 Msa0725640:intron 15.0%
!!! TAATACAATCTAACTTATTT+TGG - chr5_1:25366462-25366481 Msa0725640:intron 15.0%
!! ATTTATGTGATTTACCATTT+GGG + chr5_1:25366244-25366263 None:intergenic 20.0%
!! AATTTATGTGATTTACCATT+TGG + chr5_1:25366245-25366264 None:intergenic 20.0%
!! AATCACATAAATTGTAACTT+AGG - chr5_1:25366252-25366271 Msa0725640:intron 20.0%
!!! TGAATATTTTAACCTTTAGT+AGG - chr5_1:25366748-25366767 Msa0725640:intron 20.0%
!!! GATTTGAATGTAATGTATTT+TGG - chr5_1:25366954-25366973 Msa0725640:CDS 20.0%
!! ATTTATGTGATTTACCATTT+GGG + chr5_1:25366244-25366263 None:intergenic 20.0%
!! AATTTATGTGATTTACCATT+TGG + chr5_1:25366245-25366264 None:intergenic 20.0%
!! AATCACATAAATTGTAACTT+AGG - chr5_1:25366252-25366271 Msa0725640:intron 20.0%
!!! TGAATATTTTAACCTTTAGT+AGG - chr5_1:25366748-25366767 Msa0725640:intron 20.0%
!!! GATTTGAATGTAATGTATTT+TGG - chr5_1:25366954-25366973 Msa0725640:CDS 20.0%
!! TGCTATGAAAGTTTTTATGA+CGG - chr5_1:25366089-25366108 Msa0725640:intron 25.0%
!!! CAATCTAACTTATTTTGGAA+AGG - chr5_1:25366467-25366486 Msa0725640:intron 25.0%
! TTGTGTCTTGAAACTATTAT+AGG + chr5_1:25366511-25366530 None:intergenic 25.0%
! AAAAAAATCAAGTTACATGC+AGG + chr5_1:25366558-25366577 None:intergenic 25.0%
! TCAATGTCACAAATAACATT+AGG + chr5_1:25366583-25366602 None:intergenic 25.0%
!!! GTTATTTTTCCTTTGCTATA+AGG + chr5_1:25366911-25366930 None:intergenic 25.0%
! AAAATAACAGTATCTTGTAG+AGG - chr5_1:25366923-25366942 Msa0725640:CDS 25.0%
!!! GTAATGTATTTTGGTTATAC+AGG - chr5_1:25366963-25366982 Msa0725640:CDS 25.0%
!!! TATTTTGGTTATACAGGTAA+GGG - chr5_1:25366969-25366988 Msa0725640:CDS 25.0%
!! TGCTATGAAAGTTTTTATGA+CGG - chr5_1:25366089-25366108 Msa0725640:intron 25.0%
!!! CAATCTAACTTATTTTGGAA+AGG - chr5_1:25366467-25366486 Msa0725640:intron 25.0%
! TTGTGTCTTGAAACTATTAT+AGG + chr5_1:25366511-25366530 None:intergenic 25.0%
! AAAAAAATCAAGTTACATGC+AGG + chr5_1:25366558-25366577 None:intergenic 25.0%
! TCAATGTCACAAATAACATT+AGG + chr5_1:25366583-25366602 None:intergenic 25.0%
!!! GTTATTTTTCCTTTGCTATA+AGG + chr5_1:25366911-25366930 None:intergenic 25.0%
! AAAATAACAGTATCTTGTAG+AGG - chr5_1:25366923-25366942 Msa0725640:CDS 25.0%
!!! GTAATGTATTTTGGTTATAC+AGG - chr5_1:25366963-25366982 Msa0725640:CDS 25.0%
!!! TATTTTGGTTATACAGGTAA+GGG - chr5_1:25366969-25366988 Msa0725640:CDS 25.0%
AAACTTCAATCTTCCATGTT+TGG - chr5_1:25366164-25366183 Msa0725640:intron 30.0%
!!! AAGCTGAATATTTTGAGACA+TGG + chr5_1:25366210-25366229 None:intergenic 30.0%
TCAAAATATTCAGCTTTCCA+TGG - chr5_1:25366214-25366233 Msa0725640:intron 30.0%
! TGTTATTTGTGACATTGACT+AGG - chr5_1:25366585-25366604 Msa0725640:intron 30.0%
GGACATATTTGATTCTTGAA+TGG + chr5_1:25366635-25366654 None:intergenic 30.0%
AGCAACGATTTATCAGTTAT+AGG + chr5_1:25366656-25366675 None:intergenic 30.0%
TATAACTGATAAATCGTTGC+TGG - chr5_1:25366655-25366674 Msa0725640:intron 30.0%
AAAATCCATCCTTATAGCAA+AGG - chr5_1:25366899-25366918 Msa0725640:intron 30.0%
!! TTTTTCCTTTGCTATAAGGA+TGG + chr5_1:25366907-25366926 None:intergenic 30.0%
!!! GTATTTTGGTTATACAGGTA+AGG - chr5_1:25366968-25366987 Msa0725640:CDS 30.0%
AATCATAACATGCATGAACA+AGG - chr5_1:25366993-25367012 Msa0725640:CDS 30.0%
AAACTTCAATCTTCCATGTT+TGG - chr5_1:25366164-25366183 Msa0725640:intron 30.0%
!!! AAGCTGAATATTTTGAGACA+TGG + chr5_1:25366210-25366229 None:intergenic 30.0%
TCAAAATATTCAGCTTTCCA+TGG - chr5_1:25366214-25366233 Msa0725640:intron 30.0%
! TGTTATTTGTGACATTGACT+AGG - chr5_1:25366585-25366604 Msa0725640:intron 30.0%
GGACATATTTGATTCTTGAA+TGG + chr5_1:25366635-25366654 None:intergenic 30.0%
AGCAACGATTTATCAGTTAT+AGG + chr5_1:25366656-25366675 None:intergenic 30.0%
TATAACTGATAAATCGTTGC+TGG - chr5_1:25366655-25366674 Msa0725640:intron 30.0%
AAAATCCATCCTTATAGCAA+AGG - chr5_1:25366899-25366918 Msa0725640:intron 30.0%
!! TTTTTCCTTTGCTATAAGGA+TGG + chr5_1:25366907-25366926 None:intergenic 30.0%
!!! GTATTTTGGTTATACAGGTA+AGG - chr5_1:25366968-25366987 Msa0725640:CDS 30.0%
AATCATAACATGCATGAACA+AGG - chr5_1:25366993-25367012 Msa0725640:CDS 30.0%
TGGACTCATAATCGTAAAGT+GGG + chr5_1:25365999-25366018 None:intergenic 35.0%
TTGTAACTTAGGCTATTCTC+AGG - chr5_1:25366263-25366282 Msa0725640:intron 35.0%
! ATTTGTGACATTGACTAGGA+CGG - chr5_1:25366589-25366608 Msa0725640:intron 35.0%
TCTTGAATGGAGTTCTCAAA+GGG + chr5_1:25366622-25366641 None:intergenic 35.0%
TTCTTGAATGGAGTTCTCAA+AGG + chr5_1:25366623-25366642 None:intergenic 35.0%
! AAATCGTTGCTGGTTAAAGT+TGG - chr5_1:25366665-25366684 Msa0725640:intron 35.0%
ACACAAAGAAAGCCTACTAA+AGG + chr5_1:25366763-25366782 None:intergenic 35.0%
AAAAACCAATTCCAAGCACA+AGG + chr5_1:25366820-25366839 None:intergenic 35.0%
TGGACTCATAATCGTAAAGT+GGG + chr5_1:25365999-25366018 None:intergenic 35.0%
TTGTAACTTAGGCTATTCTC+AGG - chr5_1:25366263-25366282 Msa0725640:intron 35.0%
! ATTTGTGACATTGACTAGGA+CGG - chr5_1:25366589-25366608 Msa0725640:intron 35.0%
TCTTGAATGGAGTTCTCAAA+GGG + chr5_1:25366622-25366641 None:intergenic 35.0%
TTCTTGAATGGAGTTCTCAA+AGG + chr5_1:25366623-25366642 None:intergenic 35.0%
! AAATCGTTGCTGGTTAAAGT+TGG - chr5_1:25366665-25366684 Msa0725640:intron 35.0%
ACACAAAGAAAGCCTACTAA+AGG + chr5_1:25366763-25366782 None:intergenic 35.0%
AAAAACCAATTCCAAGCACA+AGG + chr5_1:25366820-25366839 None:intergenic 35.0%
CTGGACTCATAATCGTAAAG+TGG + chr5_1:25366000-25366019 None:intergenic 40.0%
ACGATTATGAGTCCAGAAGA+CGG - chr5_1:25366004-25366023 Msa0725640:CDS 40.0%
TTGATGAATCAACCGTCTTC+TGG + chr5_1:25366019-25366038 None:intergenic 40.0%
TAGCAGCTGAAAAATGCTCA+GGG + chr5_1:25366074-25366093 None:intergenic 40.0%
ATAGCAGCTGAAAAATGCTC+AGG + chr5_1:25366075-25366094 None:intergenic 40.0%
ATTGTGTCTCAATCAACCCA+TGG + chr5_1:25366130-25366149 None:intergenic 40.0%
CTTCAATCTTCCATGTTTGG+TGG - chr5_1:25366167-25366186 Msa0725640:intron 40.0%
!!! TTTTGAGACATGGAGTCAAG+AGG + chr5_1:25366200-25366219 None:intergenic 40.0%
TTTACCATTTGGGTCATCCA+TGG + chr5_1:25366234-25366253 None:intergenic 40.0%
! GCTGGTTAAAGTTGGTTTCA+AGG - chr5_1:25366673-25366692 Msa0725640:intron 40.0%
AGCGAAAAAATGAGCTGTGA+AGG + chr5_1:25366868-25366887 None:intergenic 40.0%
GAACAAGGAATCTCCAAAGA+AGG - chr5_1:25367008-25367027 Msa0725640:CDS 40.0%
CTTCATTTCACTGCCTTCTT+TGG + chr5_1:25367024-25367043 None:intergenic 40.0%
CAAAGAAGGCAGTGAAATGA+AGG - chr5_1:25367022-25367041 Msa0725640:CDS 40.0%
AAGGCAGTGAAATGAAGGAA+AGG - chr5_1:25367027-25367046 Msa0725640:CDS 40.0%
CTGGACTCATAATCGTAAAG+TGG + chr5_1:25366000-25366019 None:intergenic 40.0%
ACGATTATGAGTCCAGAAGA+CGG - chr5_1:25366004-25366023 Msa0725640:CDS 40.0%
TTGATGAATCAACCGTCTTC+TGG + chr5_1:25366019-25366038 None:intergenic 40.0%
TAGCAGCTGAAAAATGCTCA+GGG + chr5_1:25366074-25366093 None:intergenic 40.0%
ATAGCAGCTGAAAAATGCTC+AGG + chr5_1:25366075-25366094 None:intergenic 40.0%
ATTGTGTCTCAATCAACCCA+TGG + chr5_1:25366130-25366149 None:intergenic 40.0%
CTTCAATCTTCCATGTTTGG+TGG - chr5_1:25366167-25366186 Msa0725640:intron 40.0%
!!! TTTTGAGACATGGAGTCAAG+AGG + chr5_1:25366200-25366219 None:intergenic 40.0%
TTTACCATTTGGGTCATCCA+TGG + chr5_1:25366234-25366253 None:intergenic 40.0%
! GCTGGTTAAAGTTGGTTTCA+AGG - chr5_1:25366673-25366692 Msa0725640:intron 40.0%
AGCGAAAAAATGAGCTGTGA+AGG + chr5_1:25366868-25366887 None:intergenic 40.0%
GAACAAGGAATCTCCAAAGA+AGG - chr5_1:25367008-25367027 Msa0725640:CDS 40.0%
CTTCATTTCACTGCCTTCTT+TGG + chr5_1:25367024-25367043 None:intergenic 40.0%
CAAAGAAGGCAGTGAAATGA+AGG - chr5_1:25367022-25367041 Msa0725640:CDS 40.0%
AAGGCAGTGAAATGAAGGAA+AGG - chr5_1:25367027-25367046 Msa0725640:CDS 40.0%
CTTGCTTTCTCGGAAAACAG+TGG + chr5_1:25366049-25366068 None:intergenic 45.0%
TCTCAATCAACCCATGGCAT+GGG + chr5_1:25366124-25366143 None:intergenic 45.0%
!! TCTTCCATGTTTGGTGGTGA+AGG - chr5_1:25366173-25366192 Msa0725640:intron 45.0%
CTTTCCATGGATGACCCAAA+TGG - chr5_1:25366227-25366246 Msa0725640:intron 45.0%
GCTATTCTCAGGTGTCAGTT+GGG - chr5_1:25366274-25366293 Msa0725640:intron 45.0%
CTATTCTCAGGTGTCAGTTG+GGG - chr5_1:25366275-25366294 Msa0725640:intron 45.0%
AACCAATTCCAAGCACAAGG+AGG + chr5_1:25366817-25366836 None:intergenic 45.0%
CTTGCTTTCTCGGAAAACAG+TGG + chr5_1:25366049-25366068 None:intergenic 45.0%
TCTCAATCAACCCATGGCAT+GGG + chr5_1:25366124-25366143 None:intergenic 45.0%
!! TCTTCCATGTTTGGTGGTGA+AGG - chr5_1:25366173-25366192 Msa0725640:intron 45.0%
CTTTCCATGGATGACCCAAA+TGG - chr5_1:25366227-25366246 Msa0725640:intron 45.0%
GCTATTCTCAGGTGTCAGTT+GGG - chr5_1:25366274-25366293 Msa0725640:intron 45.0%
CTATTCTCAGGTGTCAGTTG+GGG - chr5_1:25366275-25366294 Msa0725640:intron 45.0%
AACCAATTCCAAGCACAAGG+AGG + chr5_1:25366817-25366836 None:intergenic 45.0%
!!! TAAGAAATATTTAATTTAAA+TGG - chr5_1:25366364-25366383 Msa0725640:intron 5.0%
!!! TAAGAAATATTTAATTTAAA+TGG - chr5_1:25366364-25366383 Msa0725640:intron 5.0%
AAAGTGGGATCTTCTCGCAG+AGG + chr5_1:25365984-25366003 None:intergenic 50.0%
CTCAATCAACCCATGGCATG+GGG + chr5_1:25366123-25366142 None:intergenic 50.0%
GTCTCAATCAACCCATGGCA+TGG + chr5_1:25366125-25366144 None:intergenic 50.0%
AGGACCTTCACCACCAAACA+TGG + chr5_1:25366180-25366199 None:intergenic 50.0%
GGCTATTCTCAGGTGTCAGT+TGG - chr5_1:25366273-25366292 Msa0725640:intron 50.0%
AAGCACAAGGAGGTGGAAAG+AGG + chr5_1:25366807-25366826 None:intergenic 50.0%
TCTTTCCACCTCCTTGTGCT+TGG - chr5_1:25366806-25366825 Msa0725640:CDS 50.0%
CAATTCCAAGCACAAGGAGG+TGG + chr5_1:25366814-25366833 None:intergenic 50.0%
CACCTCCTTGTGCTTGGAAT+TGG - chr5_1:25366812-25366831 Msa0725640:CDS 50.0%
AAAGTGGGATCTTCTCGCAG+AGG + chr5_1:25365984-25366003 None:intergenic 50.0%
CTCAATCAACCCATGGCATG+GGG + chr5_1:25366123-25366142 None:intergenic 50.0%
GTCTCAATCAACCCATGGCA+TGG + chr5_1:25366125-25366144 None:intergenic 50.0%
AGGACCTTCACCACCAAACA+TGG + chr5_1:25366180-25366199 None:intergenic 50.0%
GGCTATTCTCAGGTGTCAGT+TGG - chr5_1:25366273-25366292 Msa0725640:intron 50.0%
AAGCACAAGGAGGTGGAAAG+AGG + chr5_1:25366807-25366826 None:intergenic 50.0%
TCTTTCCACCTCCTTGTGCT+TGG - chr5_1:25366806-25366825 Msa0725640:CDS 50.0%
CAATTCCAAGCACAAGGAGG+TGG + chr5_1:25366814-25366833 None:intergenic 50.0%
CACCTCCTTGTGCTTGGAAT+TGG - chr5_1:25366812-25366831 Msa0725640:CDS 50.0%
GCTCAGGGCTCTTGCTTTCT+CGG + chr5_1:25366059-25366078 None:intergenic 55.0%
GCTGAACTTCCCCATGCCAT+GGG - chr5_1:25366111-25366130 Msa0725640:intron 55.0%
GCTCAGGGCTCTTGCTTTCT+CGG + chr5_1:25366059-25366078 None:intergenic 55.0%
GCTGAACTTCCCCATGCCAT+GGG - chr5_1:25366111-25366130 Msa0725640:intron 55.0%
GGCTGAACTTCCCCATGCCA+TGG - chr5_1:25366110-25366129 Msa0725640:intron 60.0%
GGCTGAACTTCCCCATGCCA+TGG - chr5_1:25366110-25366129 Msa0725640:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 25365967 25367070 25365967 ID=Msa0725640;Name=Msa0725640
chr5_1 mRNA 25365967 25367070 25365967 ID=Msa0725640-mRNA-1;Parent=Msa0725640;Name=Msa0725640-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|0|0|3|0|111
chr5_1 exon 25366905 25367070 25366905 ID=Msa0725640-mRNA-1:exon:9197;Parent=Msa0725640-mRNA-1
chr5_1 exon 25366789 25366842 25366789 ID=Msa0725640-mRNA-1:exon:9196;Parent=Msa0725640-mRNA-1
chr5_1 exon 25365967 25366082 25365967 ID=Msa0725640-mRNA-1:exon:9195;Parent=Msa0725640-mRNA-1
chr5_1 CDS 25366905 25367070 25366905 ID=Msa0725640-mRNA-1:cds;Parent=Msa0725640-mRNA-1
chr5_1 CDS 25366789 25366842 25366789 ID=Msa0725640-mRNA-1:cds;Parent=Msa0725640-mRNA-1
chr5_1 CDS 25365967 25366082 25365967 ID=Msa0725640-mRNA-1:cds;Parent=Msa0725640-mRNA-1
Gene Sequence

>Msa0725640

ATGGATGTTGCTGACCTCTGCGAGAAGATCCCACTTTACGATTATGAGTCCAGAAGACGGTTGATTCATCAAGTTAAGTCCACTGTTTTCCGAGAAAGCAAGAGCCCTGAGCATTTTTCAGCTGCTATGAAAGTTTTTATGACGGCTGAACTTCCCCATGCCATGGGTCCTCTTGACTCCATGTCTCAAAATATTCAGCTTTCCATGGATGACCCAAATGATTTGAATGTAATGTATTTTGGTTATACAGGTAAGGGAAATCATAACATGCATGAACAAGGAATCTCCAAAGAAGGCAGTGAAATGAAGGAAAGGAAACCGCAAATTGAACGATGA

Protein sequence

>Msa0725640

MDVADLCEKIPLYDYESRRRLIHQVKSTVFRESKSPEHFSAAMKVFMTAELPHAMGPLDSMSQNIQLSMDDPNDLNVMYFGYTGKGNHNMHEQGISKEGSEMKERKPQIER*