AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0845730


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0845730 MtrunA17_Chr3g0113701 57.692 52 22 0 4 55 968 1019 5.90e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0845730 AT3G11130.1 57.692 52 22 0 4 55 967 1018 2.54e-11 59.3

Find 24 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATTTCACTGCCTTCTT+TGG 0.143713 5_4:+25090483 None:intergenic
GATTTGAATGTAATGTATTT+TGG 0.199982 5_4:-25090550 Msa0845730:CDS
TTGATGAATCAACCGTCTTC+TGG 0.291397 5_4:+25091488 None:intergenic
GCTCAGGGCTCTTGCTTTCT+CGG 0.309822 5_4:+25091448 None:intergenic
GTTCCTCATCGTTCAATTTG+CGG 0.381341 5_4:+25090451 None:intergenic
ATTTATGTGATTTACCATTT+GGG 0.401363 5_4:+25091263 None:intergenic
GGCTGAACTTCCCCATGCCA+TGG 0.485955 5_4:-25091394 Msa0845730:CDS
CAAAGAAGGCAGTGAAATGA+AGG 0.488888 5_4:-25090482 Msa0845730:CDS
TCAAAATATTCAGCTTTCCA+TGG 0.490614 5_4:-25091290 Msa0845730:CDS
TCTCAATCAACCCATGGCAT+GGG 0.494707 5_4:+25091383 None:intergenic
CTGGACTCATAATCGTAAAG+TGG 0.500636 5_4:+25091507 None:intergenic
GTCTCAATCAACCCATGGCA+TGG 0.509828 5_4:+25091382 None:intergenic
TTTACCATTTGGGTCATCCA+TGG 0.522483 5_4:+25091273 None:intergenic
CTTTCCATGGATGACCCAAA+TGG 0.542785 5_4:-25091277 Msa0845730:intron
AAGGCAGTGAAATGAAGGAA+AGG 0.545158 5_4:-25090477 Msa0845730:CDS
GCTGAACTTCCCCATGCCAT+GGG 0.559299 5_4:-25091393 Msa0845730:intron
GAACAAGGAATCTCCAAAGA+AGG 0.561017 5_4:-25090496 Msa0845730:CDS
TGGACTCATAATCGTAAAGT+GGG 0.594969 5_4:+25091508 None:intergenic
CTTGCTTTCTCGGAAAACAG+TGG 0.609184 5_4:+25091458 None:intergenic
AATCATAACATGCATGAACA+AGG 0.612137 5_4:-25090511 Msa0845730:CDS
CTCAATCAACCCATGGCATG+GGG 0.619541 5_4:+25091384 None:intergenic
ACGATTATGAGTCCAGAAGA+CGG 0.640298 5_4:-25091500 Msa0845730:CDS
AAACCGCAAATTGAACGATG+AGG 0.696659 5_4:-25090454 None:intergenic
AAAGTGGGATCTTCTCGCAG+AGG 0.701756 5_4:+25091523 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAAATACTGAAAATT+TGG - chr5_4:25090819-25090838 Msa0845730:intron 15.0%
!!! TAATACAATCTAACTTATTT+TGG - chr5_4:25090951-25090970 Msa0845730:intron 15.0%
!! AACAAAAAATACTGAAAATT+TGG - chr5_4:25090819-25090838 Msa0845730:intron 15.0%
!!! TAATACAATCTAACTTATTT+TGG - chr5_4:25090951-25090970 Msa0845730:intron 15.0%
!! ATTTATGTGATTTACCATTT+GGG + chr5_4:25090733-25090752 None:intergenic 20.0%
!! AATTTATGTGATTTACCATT+TGG + chr5_4:25090734-25090753 None:intergenic 20.0%
!! AATCACATAAATTGTAACTT+AGG - chr5_4:25090741-25090760 Msa0845730:intron 20.0%
!!! TGAATATTTTAACCTTTAGT+AGG - chr5_4:25091237-25091256 Msa0845730:intron 20.0%
!!! GATTTGAATGTAATGTATTT+TGG - chr5_4:25091443-25091462 Msa0845730:CDS 20.0%
!! ATTTATGTGATTTACCATTT+GGG + chr5_4:25090733-25090752 None:intergenic 20.0%
!! AATTTATGTGATTTACCATT+TGG + chr5_4:25090734-25090753 None:intergenic 20.0%
!! AATCACATAAATTGTAACTT+AGG - chr5_4:25090741-25090760 Msa0845730:intron 20.0%
!!! TGAATATTTTAACCTTTAGT+AGG - chr5_4:25091237-25091256 Msa0845730:intron 20.0%
!!! GATTTGAATGTAATGTATTT+TGG - chr5_4:25091443-25091462 Msa0845730:CDS 20.0%
!! TGCTATGAAAGTTTTTATGA+CGG - chr5_4:25090578-25090597 Msa0845730:intron 25.0%
!!! CAATCTAACTTATTTTGGAA+AGG - chr5_4:25090956-25090975 Msa0845730:intron 25.0%
! TTGTGTCTTGAAACTATTAT+AGG + chr5_4:25091000-25091019 None:intergenic 25.0%
! AAAAAAATCAAGTTACATGC+AGG + chr5_4:25091047-25091066 None:intergenic 25.0%
! TCAATGTCACAAATAACATT+AGG + chr5_4:25091072-25091091 None:intergenic 25.0%
!!! GTTATTTTTCCTTTGCTATA+AGG + chr5_4:25091400-25091419 None:intergenic 25.0%
! AAAATAACAGTATCTTGTAG+AGG - chr5_4:25091412-25091431 Msa0845730:CDS 25.0%
!!! GTAATGTATTTTGGTTATAC+AGG - chr5_4:25091452-25091471 Msa0845730:CDS 25.0%
!!! TATTTTGGTTATACAGGTAA+GGG - chr5_4:25091458-25091477 Msa0845730:CDS 25.0%
!! TGCTATGAAAGTTTTTATGA+CGG - chr5_4:25090578-25090597 Msa0845730:intron 25.0%
!!! CAATCTAACTTATTTTGGAA+AGG - chr5_4:25090956-25090975 Msa0845730:intron 25.0%
! TTGTGTCTTGAAACTATTAT+AGG + chr5_4:25091000-25091019 None:intergenic 25.0%
! AAAAAAATCAAGTTACATGC+AGG + chr5_4:25091047-25091066 None:intergenic 25.0%
! TCAATGTCACAAATAACATT+AGG + chr5_4:25091072-25091091 None:intergenic 25.0%
!!! GTTATTTTTCCTTTGCTATA+AGG + chr5_4:25091400-25091419 None:intergenic 25.0%
! AAAATAACAGTATCTTGTAG+AGG - chr5_4:25091412-25091431 Msa0845730:CDS 25.0%
!!! GTAATGTATTTTGGTTATAC+AGG - chr5_4:25091452-25091471 Msa0845730:CDS 25.0%
!!! TATTTTGGTTATACAGGTAA+GGG - chr5_4:25091458-25091477 Msa0845730:CDS 25.0%
AAACTTCAATCTTCCATGTT+TGG - chr5_4:25090653-25090672 Msa0845730:intron 30.0%
!!! AAGCTGAATATTTTGAGACA+TGG + chr5_4:25090699-25090718 None:intergenic 30.0%
TCAAAATATTCAGCTTTCCA+TGG - chr5_4:25090703-25090722 Msa0845730:intron 30.0%
! TGTTATTTGTGACATTGACT+AGG - chr5_4:25091074-25091093 Msa0845730:intron 30.0%
GGACATATTTGATTCTTGAA+TGG + chr5_4:25091124-25091143 None:intergenic 30.0%
AGCAACGATTTATCAGTTAT+AGG + chr5_4:25091145-25091164 None:intergenic 30.0%
TATAACTGATAAATCGTTGC+TGG - chr5_4:25091144-25091163 Msa0845730:intron 30.0%
AAAATCCATCCTTATAGCAA+AGG - chr5_4:25091388-25091407 Msa0845730:intron 30.0%
!! TTTTTCCTTTGCTATAAGGA+TGG + chr5_4:25091396-25091415 None:intergenic 30.0%
!!! GTATTTTGGTTATACAGGTA+AGG - chr5_4:25091457-25091476 Msa0845730:CDS 30.0%
AATCATAACATGCATGAACA+AGG - chr5_4:25091482-25091501 Msa0845730:CDS 30.0%
AAACTTCAATCTTCCATGTT+TGG - chr5_4:25090653-25090672 Msa0845730:intron 30.0%
!!! AAGCTGAATATTTTGAGACA+TGG + chr5_4:25090699-25090718 None:intergenic 30.0%
TCAAAATATTCAGCTTTCCA+TGG - chr5_4:25090703-25090722 Msa0845730:intron 30.0%
! TGTTATTTGTGACATTGACT+AGG - chr5_4:25091074-25091093 Msa0845730:intron 30.0%
GGACATATTTGATTCTTGAA+TGG + chr5_4:25091124-25091143 None:intergenic 30.0%
AGCAACGATTTATCAGTTAT+AGG + chr5_4:25091145-25091164 None:intergenic 30.0%
TATAACTGATAAATCGTTGC+TGG - chr5_4:25091144-25091163 Msa0845730:intron 30.0%
AAAATCCATCCTTATAGCAA+AGG - chr5_4:25091388-25091407 Msa0845730:intron 30.0%
!! TTTTTCCTTTGCTATAAGGA+TGG + chr5_4:25091396-25091415 None:intergenic 30.0%
!!! GTATTTTGGTTATACAGGTA+AGG - chr5_4:25091457-25091476 Msa0845730:CDS 30.0%
AATCATAACATGCATGAACA+AGG - chr5_4:25091482-25091501 Msa0845730:CDS 30.0%
TGGACTCATAATCGTAAAGT+GGG + chr5_4:25090488-25090507 None:intergenic 35.0%
TTGTAACTTAGGCTATTCTC+AGG - chr5_4:25090752-25090771 Msa0845730:intron 35.0%
! ATTTGTGACATTGACTAGGA+CGG - chr5_4:25091078-25091097 Msa0845730:intron 35.0%
TCTTGAATGGAGTTCTCAAA+GGG + chr5_4:25091111-25091130 None:intergenic 35.0%
TTCTTGAATGGAGTTCTCAA+AGG + chr5_4:25091112-25091131 None:intergenic 35.0%
! AAATCGTTGCTGGTTAAAGT+TGG - chr5_4:25091154-25091173 Msa0845730:intron 35.0%
ACACAAAGAAAGCCTACTAA+AGG + chr5_4:25091252-25091271 None:intergenic 35.0%
AAAAACCAATTCCAAGCACA+AGG + chr5_4:25091309-25091328 None:intergenic 35.0%
TGGACTCATAATCGTAAAGT+GGG + chr5_4:25090488-25090507 None:intergenic 35.0%
TTGTAACTTAGGCTATTCTC+AGG - chr5_4:25090752-25090771 Msa0845730:intron 35.0%
! ATTTGTGACATTGACTAGGA+CGG - chr5_4:25091078-25091097 Msa0845730:intron 35.0%
TCTTGAATGGAGTTCTCAAA+GGG + chr5_4:25091111-25091130 None:intergenic 35.0%
TTCTTGAATGGAGTTCTCAA+AGG + chr5_4:25091112-25091131 None:intergenic 35.0%
! AAATCGTTGCTGGTTAAAGT+TGG - chr5_4:25091154-25091173 Msa0845730:intron 35.0%
ACACAAAGAAAGCCTACTAA+AGG + chr5_4:25091252-25091271 None:intergenic 35.0%
AAAAACCAATTCCAAGCACA+AGG + chr5_4:25091309-25091328 None:intergenic 35.0%
CTGGACTCATAATCGTAAAG+TGG + chr5_4:25090489-25090508 None:intergenic 40.0%
ACGATTATGAGTCCAGAAGA+CGG - chr5_4:25090493-25090512 Msa0845730:CDS 40.0%
TTGATGAATCAACCGTCTTC+TGG + chr5_4:25090508-25090527 None:intergenic 40.0%
TAGCAGCTGAAAAATGCTCA+GGG + chr5_4:25090563-25090582 None:intergenic 40.0%
ATAGCAGCTGAAAAATGCTC+AGG + chr5_4:25090564-25090583 None:intergenic 40.0%
ATTGTGTCTCAATCAACCCA+TGG + chr5_4:25090619-25090638 None:intergenic 40.0%
CTTCAATCTTCCATGTTTGG+TGG - chr5_4:25090656-25090675 Msa0845730:intron 40.0%
!!! TTTTGAGACATGGAGTCAAG+AGG + chr5_4:25090689-25090708 None:intergenic 40.0%
TTTACCATTTGGGTCATCCA+TGG + chr5_4:25090723-25090742 None:intergenic 40.0%
! GCTGGTTAAAGTTGGTTTCA+AGG - chr5_4:25091162-25091181 Msa0845730:intron 40.0%
AGCGAAAAAATGAGCTGTGA+AGG + chr5_4:25091357-25091376 None:intergenic 40.0%
GAACAAGGAATCTCCAAAGA+AGG - chr5_4:25091497-25091516 Msa0845730:CDS 40.0%
CTTCATTTCACTGCCTTCTT+TGG + chr5_4:25091513-25091532 None:intergenic 40.0%
CAAAGAAGGCAGTGAAATGA+AGG - chr5_4:25091511-25091530 Msa0845730:CDS 40.0%
AAGGCAGTGAAATGAAGGAA+AGG - chr5_4:25091516-25091535 Msa0845730:CDS 40.0%
CTGGACTCATAATCGTAAAG+TGG + chr5_4:25090489-25090508 None:intergenic 40.0%
ACGATTATGAGTCCAGAAGA+CGG - chr5_4:25090493-25090512 Msa0845730:CDS 40.0%
TTGATGAATCAACCGTCTTC+TGG + chr5_4:25090508-25090527 None:intergenic 40.0%
TAGCAGCTGAAAAATGCTCA+GGG + chr5_4:25090563-25090582 None:intergenic 40.0%
ATAGCAGCTGAAAAATGCTC+AGG + chr5_4:25090564-25090583 None:intergenic 40.0%
ATTGTGTCTCAATCAACCCA+TGG + chr5_4:25090619-25090638 None:intergenic 40.0%
CTTCAATCTTCCATGTTTGG+TGG - chr5_4:25090656-25090675 Msa0845730:intron 40.0%
!!! TTTTGAGACATGGAGTCAAG+AGG + chr5_4:25090689-25090708 None:intergenic 40.0%
TTTACCATTTGGGTCATCCA+TGG + chr5_4:25090723-25090742 None:intergenic 40.0%
! GCTGGTTAAAGTTGGTTTCA+AGG - chr5_4:25091162-25091181 Msa0845730:intron 40.0%
AGCGAAAAAATGAGCTGTGA+AGG + chr5_4:25091357-25091376 None:intergenic 40.0%
GAACAAGGAATCTCCAAAGA+AGG - chr5_4:25091497-25091516 Msa0845730:CDS 40.0%
CTTCATTTCACTGCCTTCTT+TGG + chr5_4:25091513-25091532 None:intergenic 40.0%
CAAAGAAGGCAGTGAAATGA+AGG - chr5_4:25091511-25091530 Msa0845730:CDS 40.0%
AAGGCAGTGAAATGAAGGAA+AGG - chr5_4:25091516-25091535 Msa0845730:CDS 40.0%
CTTGCTTTCTCGGAAAACAG+TGG + chr5_4:25090538-25090557 None:intergenic 45.0%
TCTCAATCAACCCATGGCAT+GGG + chr5_4:25090613-25090632 None:intergenic 45.0%
!! TCTTCCATGTTTGGTGGTGA+AGG - chr5_4:25090662-25090681 Msa0845730:intron 45.0%
CTTTCCATGGATGACCCAAA+TGG - chr5_4:25090716-25090735 Msa0845730:intron 45.0%
GCTATTCTCAGGTGTCAGTT+GGG - chr5_4:25090763-25090782 Msa0845730:intron 45.0%
CTATTCTCAGGTGTCAGTTG+GGG - chr5_4:25090764-25090783 Msa0845730:intron 45.0%
AACCAATTCCAAGCACAAGG+AGG + chr5_4:25091306-25091325 None:intergenic 45.0%
CTTGCTTTCTCGGAAAACAG+TGG + chr5_4:25090538-25090557 None:intergenic 45.0%
TCTCAATCAACCCATGGCAT+GGG + chr5_4:25090613-25090632 None:intergenic 45.0%
!! TCTTCCATGTTTGGTGGTGA+AGG - chr5_4:25090662-25090681 Msa0845730:intron 45.0%
CTTTCCATGGATGACCCAAA+TGG - chr5_4:25090716-25090735 Msa0845730:intron 45.0%
GCTATTCTCAGGTGTCAGTT+GGG - chr5_4:25090763-25090782 Msa0845730:intron 45.0%
CTATTCTCAGGTGTCAGTTG+GGG - chr5_4:25090764-25090783 Msa0845730:intron 45.0%
AACCAATTCCAAGCACAAGG+AGG + chr5_4:25091306-25091325 None:intergenic 45.0%
!!! TAAGAAATATTTAATTTAAA+TGG - chr5_4:25090853-25090872 Msa0845730:intron 5.0%
!!! TAAGAAATATTTAATTTAAA+TGG - chr5_4:25090853-25090872 Msa0845730:intron 5.0%
AAAGTGGGATCTTCTCGCAG+AGG + chr5_4:25090473-25090492 None:intergenic 50.0%
CTCAATCAACCCATGGCATG+GGG + chr5_4:25090612-25090631 None:intergenic 50.0%
GTCTCAATCAACCCATGGCA+TGG + chr5_4:25090614-25090633 None:intergenic 50.0%
AGGACCTTCACCACCAAACA+TGG + chr5_4:25090669-25090688 None:intergenic 50.0%
GGCTATTCTCAGGTGTCAGT+TGG - chr5_4:25090762-25090781 Msa0845730:intron 50.0%
AAGCACAAGGAGGTGGAAAG+AGG + chr5_4:25091296-25091315 None:intergenic 50.0%
TCTTTCCACCTCCTTGTGCT+TGG - chr5_4:25091295-25091314 Msa0845730:CDS 50.0%
CAATTCCAAGCACAAGGAGG+TGG + chr5_4:25091303-25091322 None:intergenic 50.0%
CACCTCCTTGTGCTTGGAAT+TGG - chr5_4:25091301-25091320 Msa0845730:CDS 50.0%
AAAGTGGGATCTTCTCGCAG+AGG + chr5_4:25090473-25090492 None:intergenic 50.0%
CTCAATCAACCCATGGCATG+GGG + chr5_4:25090612-25090631 None:intergenic 50.0%
GTCTCAATCAACCCATGGCA+TGG + chr5_4:25090614-25090633 None:intergenic 50.0%
AGGACCTTCACCACCAAACA+TGG + chr5_4:25090669-25090688 None:intergenic 50.0%
GGCTATTCTCAGGTGTCAGT+TGG - chr5_4:25090762-25090781 Msa0845730:intron 50.0%
AAGCACAAGGAGGTGGAAAG+AGG + chr5_4:25091296-25091315 None:intergenic 50.0%
TCTTTCCACCTCCTTGTGCT+TGG - chr5_4:25091295-25091314 Msa0845730:CDS 50.0%
CAATTCCAAGCACAAGGAGG+TGG + chr5_4:25091303-25091322 None:intergenic 50.0%
CACCTCCTTGTGCTTGGAAT+TGG - chr5_4:25091301-25091320 Msa0845730:CDS 50.0%
GCTCAGGGCTCTTGCTTTCT+CGG + chr5_4:25090548-25090567 None:intergenic 55.0%
GCTGAACTTCCCCATGCCAT+GGG - chr5_4:25090600-25090619 Msa0845730:intron 55.0%
GCTCAGGGCTCTTGCTTTCT+CGG + chr5_4:25090548-25090567 None:intergenic 55.0%
GCTGAACTTCCCCATGCCAT+GGG - chr5_4:25090600-25090619 Msa0845730:intron 55.0%
GGCTGAACTTCCCCATGCCA+TGG - chr5_4:25090599-25090618 Msa0845730:intron 60.0%
GGCTGAACTTCCCCATGCCA+TGG - chr5_4:25090599-25090618 Msa0845730:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 25090456 25091559 25090456 ID=Msa0845730;Name=Msa0845730
chr5_4 mRNA 25090456 25091559 25090456 ID=Msa0845730-mRNA-1;Parent=Msa0845730;Name=Msa0845730-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|0|0|3|0|111
chr5_4 exon 25091394 25091559 25091394 ID=Msa0845730-mRNA-1:exon:10048;Parent=Msa0845730-mRNA-1
chr5_4 exon 25091278 25091331 25091278 ID=Msa0845730-mRNA-1:exon:10047;Parent=Msa0845730-mRNA-1
chr5_4 exon 25090456 25090571 25090456 ID=Msa0845730-mRNA-1:exon:10046;Parent=Msa0845730-mRNA-1
chr5_4 CDS 25091394 25091559 25091394 ID=Msa0845730-mRNA-1:cds;Parent=Msa0845730-mRNA-1
chr5_4 CDS 25091278 25091331 25091278 ID=Msa0845730-mRNA-1:cds;Parent=Msa0845730-mRNA-1
chr5_4 CDS 25090456 25090571 25090456 ID=Msa0845730-mRNA-1:cds;Parent=Msa0845730-mRNA-1
Gene Sequence

>Msa0845730

ATGGATGTTGCTGACCTCTGCGAGAAGATCCCACTTTACGATTATGAGTCCAGAAGACGGTTGATTCATCAAGTTAAGTCCACTGTTTTCCGAGAAAGCAAGAGCCCTGAGCATTTTTCAGCTGCTATGAAAGTTTTTATGACGGCTGAACTTCCCCATGCCATGGGTCCTCTTGACTCCATGTCTCAAAATATTCAGCTTTCCATGGATGACCCAAATGATTTGAATGTAATGTATTTTGGTTATACAGGTAAGGGAAATCATAACATGCATGAACAAGGAATCTCCAAAGAAGGCAGTGAAATGAAGGAAAGGAAACCGCAAATTGAACGATGA

Protein sequence

>Msa0845730

MDVADLCEKIPLYDYESRRRLIHQVKSTVFRESKSPEHFSAAMKVFMTAELPHAMGPLDSMSQNIQLSMDDPNDLNVMYFGYTGKGNHNMHEQGISKEGSEMKERKPQIER*