AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0736200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0736200 MtrunA17_Chr5g0433081 72.093 172 31 4 26 185 90 256 7.73e-77 243
Msa0736200 MtrunA17_Chr6g0469611 68.085 47 15 0 67 113 17 63 6.09e-15 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0736200 AT5G51340.1 45.856 181 81 3 12 182 78 251 9.99e-42 149

Find 68 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTGAATCAGTATATTT+TGG 0.103251 5_1:+52287582 Msa0736200:three_prime_UTR
GCAATGGGATGATGATAATT+TGG 0.276465 5_1:+52287085 Msa0736200:CDS
ATTAAACAAACAAATTGTTT+TGG 0.285895 5_1:-52284872 None:intergenic
TTAATAAATGCAATGAGATT+TGG 0.289386 5_1:+52287120 Msa0736200:CDS
TTCGGTACGTGGTACGAATT+CGG 0.293951 5_1:+52287642 Msa0736200:three_prime_UTR
CTTGATCTTGCTGCTTCTGC+TGG 0.344677 5_1:+52284979 Msa0736200:CDS
ATGAATGGGTTCAGTTCAAC+TGG 0.355967 5_1:+52287552 Msa0736200:three_prime_UTR
TAATAAATGCAATGAGATTT+GGG 0.359630 5_1:+52287121 Msa0736200:CDS
TATCATCATCCCATTGCATT+AGG 0.360966 5_1:-52287079 None:intergenic
CTTTAGATCAAAGTCTTTCC+AGG 0.366141 5_1:+52287457 Msa0736200:three_prime_UTR
ACAGGCATATCCACATTCTA+AGG 0.382816 5_1:-52286562 None:intergenic
TCGGTACGTGGTACGAATTC+GGG 0.396302 5_1:+52287643 Msa0736200:three_prime_UTR
TTCGCCACTCTTAAAGAATT+CGG 0.416636 5_1:+52287673 Msa0736200:three_prime_UTR
TAAAGAATTCGGTACGCAAT+GGG 0.429485 5_1:+52287684 Msa0736200:three_prime_UTR
GCAAATGCTTCGTCAATTGA+AGG 0.429507 5_1:+52286516 Msa0736200:CDS
AGTACACTACTCAGGGTTGC+AGG 0.431855 5_1:+52286593 Msa0736200:CDS
AATTGCGAACCGTGTGACTA+TGG 0.433159 5_1:-52287616 None:intergenic
TCGTTGTAAAACAGCAAGCC+AGG 0.455989 5_1:-52287297 None:intergenic
CCAGGTCTGATCTTCACTAC+AGG 0.456609 5_1:+52287475 Msa0736200:three_prime_UTR
TGCACCCCATGTCGACAATC+TGG 0.463134 5_1:+52287368 Msa0736200:CDS
GTATACAGAGTCGGTACGCT+TGG 0.480993 5_1:+52287707 Msa0736200:three_prime_UTR
CATCATCCCATTGCATTAGG+TGG 0.483400 5_1:-52287076 None:intergenic
CTTGCTGCTTCTGCTGGAAG+TGG 0.486593 5_1:+52284985 Msa0736200:CDS
TTCACTAGCTCGTGCATATG+TGG 0.490930 5_1:-52287423 None:intergenic
TATACAGAGTCGGTACGCTT+GGG 0.512097 5_1:+52287708 Msa0736200:three_prime_UTR
TCTAAAATAATAAATTTGTC+CGG 0.516532 5_1:+52287942 Msa0736200:three_prime_UTR
CAGCATCCAGATTGTCGACA+TGG 0.524321 5_1:-52287374 None:intergenic
CGTCAATTGAAGGAGACGAC+TGG 0.526408 5_1:+52286526 Msa0736200:CDS
ACATTGGCCCAAGTCGGGAC+CGG 0.531293 5_1:-52287961 None:intergenic
GAAGGATTTCAACAAAGTTG+TGG 0.533454 5_1:+52286469 Msa0736200:intron
AACAGCAGAGAAACATGAAT+GGG 0.538114 5_1:+52287538 Msa0736200:three_prime_UTR
TCGCAATTCACTTCGGTACG+TGG 0.539166 5_1:+52287631 Msa0736200:three_prime_UTR
TGCATGCCCACCTAATGCAA+TGG 0.541142 5_1:+52287069 Msa0736200:CDS
TAACAGCAGAGAAACATGAA+TGG 0.543372 5_1:+52287537 Msa0736200:three_prime_UTR
GTCAATTGAAGGAGACGACT+GGG 0.545161 5_1:+52286527 Msa0736200:CDS
GCATGCCCACCTAATGCAAT+GGG 0.550739 5_1:+52287070 Msa0736200:CDS
AAACTCTTTAATTTAATACT+CGG 0.553860 5_1:+52287904 Msa0736200:three_prime_UTR
AGCATCCAGATTGTCGACAT+GGG 0.556283 5_1:-52287373 None:intergenic
TTAAAGAATTCGGTACGCAA+TGG 0.557099 5_1:+52287683 Msa0736200:three_prime_UTR
TTTGGAACAAGGGTTGGAGG+AGG 0.557899 5_1:-52284854 None:intergenic
GCAGCACTGTCGGAAAAGAA+AGG 0.558566 5_1:+52287504 Msa0736200:three_prime_UTR
GCTACTGAAGTACACTACTC+AGG 0.565081 5_1:+52286585 Msa0736200:CDS
CAGGTCTGATCTTCACTACA+GGG 0.570244 5_1:+52287476 Msa0736200:three_prime_UTR
CCTGTAGTGAAGATCAGACC+TGG 0.572154 5_1:-52287475 None:intergenic
CAATCTGGATGCTGTTGTGA+AGG 0.573935 5_1:+52287383 Msa0736200:CDS
GTAGTGTACTTCAGTAGCAC+AGG 0.575993 5_1:-52286580 None:intergenic
CAGGGAAAGAGCAGCACTGT+CGG 0.578539 5_1:+52287494 Msa0736200:three_prime_UTR
ATCATCCCATTGCATTAGGT+GGG 0.582466 5_1:-52287075 None:intergenic
GTGGGCATGCAATAATAAAG+TGG 0.586982 5_1:-52287057 None:intergenic
ACACGGTTCGCAATTCACTT+CGG 0.587534 5_1:+52287624 Msa0736200:three_prime_UTR
TGGGTTCAGTTCAACTGGGA+GGG 0.588991 5_1:+52287557 Msa0736200:three_prime_UTR
CGTACCGAATTCTTTAAGAG+TGG 0.590151 5_1:-52287677 None:intergenic
TCAGTCTCCGCCTTAGAATG+TGG 0.592713 5_1:+52286552 Msa0736200:CDS
CGCAATGGGGTATACAGAGT+CGG 0.602972 5_1:+52287698 Msa0736200:three_prime_UTR
ATGATAATGATGAAGAATGA+TGG 0.604892 5_1:-52284812 None:intergenic
CGTTGTAAAACAGCAAGCCA+GGG 0.613338 5_1:-52287296 None:intergenic
CTACTGAAGTACACTACTCA+GGG 0.617317 5_1:+52286586 Msa0736200:CDS
ATAATGATGAAGAATGATGG+TGG 0.635767 5_1:-52284809 None:intergenic
ACATATGCACGAGCTAGTGA+AGG 0.647182 5_1:+52287425 Msa0736200:three_prime_UTR
GTGGTACGAATTCGGGTATG+CGG 0.661892 5_1:+52287650 Msa0736200:three_prime_UTR
GCATCCAGATTGTCGACATG+GGG 0.666878 5_1:-52287372 None:intergenic
AAAGAATTCGGTACGCAATG+GGG 0.673248 5_1:+52287685 Msa0736200:three_prime_UTR
GGCATATCCACATTCTAAGG+CGG 0.674816 5_1:-52286559 None:intergenic
ATGGGTTCAGTTCAACTGGG+AGG 0.683685 5_1:+52287556 Msa0736200:three_prime_UTR
TGAATGGGTTCAGTTCAACT+GGG 0.692065 5_1:+52287553 Msa0736200:three_prime_UTR
TCAATTGAAGGAGACGACTG+GGG 0.696183 5_1:+52286528 Msa0736200:CDS
TGCACATATTCTATCGAACG+CGG 0.728324 5_1:+52287325 Msa0736200:CDS
ATGGCAGGACCATAGTCACA+CGG 0.739715 5_1:+52287607 Msa0736200:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAAAATTATTAAAA+AGG - chr5_1:52286767-52286786 None:intergenic 0.0%
!!! ATTAAACAAACAAATTGTTT+TGG - chr5_1:52284875-52284894 None:intergenic 15.0%
!!! TTTAATGATGTTTTTTTTCT+GGG + chr5_1:52284889-52284908 Msa0736200:intron 15.0%
!!! TGATTTTTTTTAGTATCTTT+TGG + chr5_1:52285040-52285059 Msa0736200:intron 15.0%
!!! CATTTTTTTAAAACATTGAA+TGG + chr5_1:52285487-52285506 Msa0736200:intron 15.0%
!! TTTCTTTAATTCATAAATTC+TGG + chr5_1:52286322-52286341 Msa0736200:intron 15.0%
!! TTCTTTAATTCATAAATTCT+GGG + chr5_1:52286323-52286342 Msa0736200:intron 15.0%
!! TTAAATTAAAAAAGGAATCA+TGG - chr5_1:52287255-52287274 None:intergenic 15.0%
!!! AAACTCTTTAATTTAATACT+CGG + chr5_1:52287904-52287923 Msa0736200:three_prime_UTR 15.0%
!!! TCTAAAATAATAAATTTGTC+CGG + chr5_1:52287942-52287961 Msa0736200:three_prime_UTR 15.0%
!!! GTTTAATGATGTTTTTTTTC+TGG + chr5_1:52284888-52284907 Msa0736200:intron 20.0%
!!! GAATTTAAGGTTTTAAATTG+TGG + chr5_1:52285069-52285088 Msa0736200:intron 20.0%
!! AGGTAACAAAATATAAAAAG+AGG - chr5_1:52285256-52285275 None:intergenic 20.0%
!!! TTTTTGTAGCAAATTTTGTA+TGG + chr5_1:52285568-52285587 Msa0736200:intron 20.0%
!!! AACAATGACTTTTGTTTTTT+AGG + chr5_1:52285648-52285667 Msa0736200:intron 20.0%
!! AGCTTATCAGAATTTATAAA+TGG - chr5_1:52285885-52285904 None:intergenic 20.0%
!! TTTATAAATTCTGATAAGCT+TGG + chr5_1:52285885-52285904 Msa0736200:intron 20.0%
!! AGCATAAATGATATAAGATA+TGG - chr5_1:52286106-52286125 None:intergenic 20.0%
!! TCTGATAATATATATGTCAA+AGG + chr5_1:52286256-52286275 Msa0736200:intron 20.0%
!!! TTTTTTGTTTATCTTGTTGA+AGG + chr5_1:52286451-52286470 Msa0736200:intron 20.0%
!! ACTTAATTCTTACAACTAAT+AGG + chr5_1:52286691-52286710 Msa0736200:intron 20.0%
!! AAGTGATGAGTTAAAAATAA+TGG - chr5_1:52286865-52286884 None:intergenic 20.0%
!!! TTCTAGTTTGCTAAAATAAA+TGG + chr5_1:52286953-52286972 Msa0736200:intron 20.0%
!! ATAATATTATAGCAAACTCT+CGG - chr5_1:52286992-52287011 None:intergenic 20.0%
!! TTAATAAATGCAATGAGATT+TGG + chr5_1:52287120-52287139 Msa0736200:CDS 20.0%
!! TAATAAATGCAATGAGATTT+GGG + chr5_1:52287121-52287140 Msa0736200:CDS 20.0%
!! GCGTTAGATTAAATTAAAAA+AGG - chr5_1:52287263-52287282 None:intergenic 20.0%
!!! TATCTTGAATCAGTATATTT+TGG + chr5_1:52287582-52287601 Msa0736200:three_prime_UTR 20.0%
!!! ATCTTGAATCAGTATATTTT+GGG + chr5_1:52287583-52287602 Msa0736200:three_prime_UTR 20.0%
!!! ATAACTCTGAATTTTTGTAA+AGG - chr5_1:52287851-52287870 None:intergenic 20.0%
!! ATGATAATGATGAAGAATGA+TGG - chr5_1:52284815-52284834 None:intergenic 25.0%
!!! AACAAATTGTTTTGGAACAA+GGG - chr5_1:52284867-52284886 None:intergenic 25.0%
!!! AAACAAATTGTTTTGGAACA+AGG - chr5_1:52284868-52284887 None:intergenic 25.0%
!! TTTTAGTATCTTTTGGATTC+TGG + chr5_1:52285047-52285066 Msa0736200:intron 25.0%
!! AAGATTTGTAACAGTGATTT+TGG + chr5_1:52285172-52285191 Msa0736200:intron 25.0%
!! TCAAAGTTTCTGATATTTTG+CGG + chr5_1:52285203-52285222 Msa0736200:intron 25.0%
! TTAGATATTGCAGAGAATTA+CGG + chr5_1:52285370-52285389 Msa0736200:intron 25.0%
! TTTCAATTGTAGTCAAATTG+CGG + chr5_1:52285418-52285437 Msa0736200:intron 25.0%
!!! TACCATCTTTTTTCAACAAT+AGG + chr5_1:52285512-52285531 Msa0736200:intron 25.0%
!!! ACCATCTTTTTTCAACAATA+GGG + chr5_1:52285513-52285532 Msa0736200:intron 25.0%
!!! TCTTGCTTTCTTTTAGTTAT+TGG + chr5_1:52285682-52285701 Msa0736200:intron 25.0%
!!! TATAACAAGTTTTTCAGACT+TGG - chr5_1:52285792-52285811 None:intergenic 25.0%
! ATTTAGTATTTACCATACAC+AGG - chr5_1:52285984-52286003 None:intergenic 25.0%
! AATTAAGTGAACTGATGAAT+CGG - chr5_1:52286679-52286698 None:intergenic 25.0%
!! GATGAGTTAAAAATAATGGA+AGG - chr5_1:52286861-52286880 None:intergenic 25.0%
!! TTTTATACCTGCTTCTATTA+TGG + chr5_1:52286890-52286909 Msa0736200:intron 25.0%
!!! CTGTCTGTTGTTTTTTTTAA+TGG + chr5_1:52287019-52287038 Msa0736200:intron 25.0%
! ATAAAGTGGTAAAGAATATC+TGG - chr5_1:52287046-52287065 None:intergenic 25.0%
! ACAAAAATTCAGAGTTATAG+AGG + chr5_1:52287853-52287872 Msa0736200:three_prime_UTR 25.0%
ATAATGATGAAGAATGATGG+TGG - chr5_1:52284812-52284831 None:intergenic 30.0%
TGGGTAGTAATTATTGTTGA+AGG + chr5_1:52284908-52284927 Msa0736200:intron 30.0%
CACTTCAACTTAAAACAAGA+CGG - chr5_1:52284939-52284958 None:intergenic 30.0%
!! GTTGAAGTGTTATCATTTTG+TGG + chr5_1:52284950-52284969 Msa0736200:intron 30.0%
!!! CTTTTGGATTCTGGAATTTA+AGG + chr5_1:52285056-52285075 Msa0736200:intron 30.0%
! TTTTCGATATTGCAAGAACT+AGG - chr5_1:52285276-52285295 None:intergenic 30.0%
! TATCTAAGATCACAACAAGA+TGG - chr5_1:52285357-52285376 None:intergenic 30.0%
ACCCTATTGTTGAAAAAAGA+TGG - chr5_1:52285517-52285536 None:intergenic 30.0%
CAATATAACATCCTCATCTA+TGG + chr5_1:52285711-52285730 Msa0736200:intron 30.0%
TTTCCTTTACAGTTTACACT+TGG - chr5_1:52286063-52286082 None:intergenic 30.0%
! TTTTCACATATTTGCACCAT+TGG + chr5_1:52286134-52286153 Msa0736200:intron 30.0%
ATTTAGTAGTATGAGACCAA+TGG - chr5_1:52286153-52286172 None:intergenic 30.0%
AAATGTAGGTATAGCTGTAT+AGG - chr5_1:52286191-52286210 None:intergenic 30.0%
! GAAAGTAATAGGTGATTTTC+AGG - chr5_1:52286218-52286237 None:intergenic 30.0%
! ATTCATAAATTCTGGGCTTT+TGG + chr5_1:52286330-52286349 Msa0736200:intron 30.0%
! TTAAAGATAAAGCATCCAGA+AGG - chr5_1:52286722-52286741 None:intergenic 30.0%
AATGGAAGGAAAGAAGAAAT+GGG - chr5_1:52286847-52286866 None:intergenic 30.0%
TAATGGAAGGAAAGAAGAAA+TGG - chr5_1:52286848-52286867 None:intergenic 30.0%
TAAATTGCCATAATAGAAGC+AGG - chr5_1:52286900-52286919 None:intergenic 30.0%
ACATTAACACTCACTTTATC+TGG - chr5_1:52287158-52287177 None:intergenic 30.0%
!! GAATCAGTATATTTTGGGAA+TGG + chr5_1:52287588-52287607 Msa0736200:three_prime_UTR 30.0%
ATGGAAGCAGTAAAAATGTC+TGG + chr5_1:52284750-52284769 Msa0736200:CDS 35.0%
!!! AATTGTTTTGGAACAAGGGT+TGG - chr5_1:52284863-52284882 None:intergenic 35.0%
! TTTTAAATTGTGGCTGCATG+TGG + chr5_1:52285079-52285098 Msa0736200:intron 35.0%
TTTAAATTGTGGCTGCATGT+GGG + chr5_1:52285080-52285099 Msa0736200:intron 35.0%
TGTGTTCTTTGGTCAACAAT+AGG + chr5_1:52285139-52285158 Msa0736200:intron 35.0%
GTGTTCTTTGGTCAACAATA+GGG + chr5_1:52285140-52285159 Msa0736200:intron 35.0%
!! AAAAGAGGCATCATTGATCA+AGG - chr5_1:52285241-52285260 None:intergenic 35.0%
AAATATCCAAGACCGCAATT+TGG - chr5_1:52285314-52285333 None:intergenic 35.0%
ATTACGGTCAAACTCAATTG+TGG + chr5_1:52285386-52285405 Msa0736200:intron 35.0%
! CTCAATCACGACATGATTTT+TGG - chr5_1:52285453-52285472 None:intergenic 35.0%
CAAAAATCATGTCGTGATTG+AGG + chr5_1:52285451-52285470 Msa0736200:intron 35.0%
!!! ATAGGGTTTTAAGCAAAGCT+TGG + chr5_1:52285530-52285549 Msa0736200:intron 35.0%
CTCTACGATCACAAATAACA+AGG + chr5_1:52285591-52285610 Msa0736200:intron 35.0%
!! AAAGGTATTGTCCATAGATG+AGG - chr5_1:52285725-52285744 None:intergenic 35.0%
ATCTATGGACAATACCTTTG+TGG + chr5_1:52285726-52285745 Msa0736200:intron 35.0%
TCTATGGACAATACCTTTGT+GGG + chr5_1:52285727-52285746 Msa0736200:intron 35.0%
!! AACAAGTTTTTCAGACTTGG+AGG - chr5_1:52285789-52285808 None:intergenic 35.0%
CAGACTTCAGTTAAAACATG+CGG - chr5_1:52285819-52285838 None:intergenic 35.0%
ACACCAAGTGTAAACTGTAA+AGG + chr5_1:52286057-52286076 Msa0736200:intron 35.0%
GGTGCAAATATGTGAAAAGA+GGG - chr5_1:52286132-52286151 None:intergenic 35.0%
TGGTGCAAATATGTGAAAAG+AGG - chr5_1:52286133-52286152 None:intergenic 35.0%
ACTCGTGTGAAGAAAGTAAT+AGG - chr5_1:52286229-52286248 None:intergenic 35.0%
GAAGGATTTCAACAAAGTTG+TGG + chr5_1:52286469-52286488 Msa0736200:intron 35.0%
AACTAATAGGTAGAACCTTC+TGG + chr5_1:52286704-52286723 Msa0736200:intron 35.0%
TATCATCATCCCATTGCATT+AGG - chr5_1:52287082-52287101 None:intergenic 35.0%
GCAATGGGATGATGATAATT+TGG + chr5_1:52287085-52287104 Msa0736200:CDS 35.0%
CTTTAGATCAAAGTCTTTCC+AGG + chr5_1:52287457-52287476 Msa0736200:three_prime_UTR 35.0%
TAACAGCAGAGAAACATGAA+TGG + chr5_1:52287537-52287556 Msa0736200:three_prime_UTR 35.0%
AACAGCAGAGAAACATGAAT+GGG + chr5_1:52287538-52287557 Msa0736200:three_prime_UTR 35.0%
TTCGCCACTCTTAAAGAATT+CGG + chr5_1:52287673-52287692 Msa0736200:three_prime_UTR 35.0%
TTAAAGAATTCGGTACGCAA+TGG + chr5_1:52287683-52287702 Msa0736200:three_prime_UTR 35.0%
TAAAGAATTCGGTACGCAAT+GGG + chr5_1:52287684-52287703 Msa0736200:three_prime_UTR 35.0%
TTAAATTGTGGCTGCATGTG+GGG + chr5_1:52285081-52285100 Msa0736200:intron 40.0%
TGTGCATGAGATGTGTTCTT+TGG + chr5_1:52285128-52285147 Msa0736200:intron 40.0%
!! TGTAACAGTGATTTTGGCAG+TGG + chr5_1:52285178-52285197 Msa0736200:intron 40.0%
TATCGAAAACTGTTATGCCG+CGG + chr5_1:52285287-52285306 Msa0736200:intron 40.0%
ACAACAAGATGGAAACAGAC+AGG - chr5_1:52285346-52285365 None:intergenic 40.0%
!!! GACATGATTTTTGGTCTCTG+CGG - chr5_1:52285444-52285463 None:intergenic 40.0%
AAAGCTTGGCAAACACACTT+TGG + chr5_1:52285544-52285563 Msa0736200:intron 40.0%
TCAGATATTGCAACCCACAA+AGG - chr5_1:52285743-52285762 None:intergenic 40.0%
!! AAGTTTTTCAGACTTGGAGG+AGG - chr5_1:52285786-52285805 None:intergenic 40.0%
GTGTGTAAAAACAGTAGCTC+TGG + chr5_1:52285958-52285977 Msa0736200:intron 40.0%
!! GATTTTCAGGCAGGAAATGT+AGG - chr5_1:52286205-52286224 None:intergenic 40.0%
!! GTAATAGGTGATTTTCAGGC+AGG - chr5_1:52286214-52286233 None:intergenic 40.0%
GCAAATGCTTCGTCAATTGA+AGG + chr5_1:52286516-52286535 Msa0736200:CDS 40.0%
ACAGGCATATCCACATTCTA+AGG - chr5_1:52286565-52286584 None:intergenic 40.0%
CTACTGAAGTACACTACTCA+GGG + chr5_1:52286586-52286605 Msa0736200:CDS 40.0%
!!! GGTTGCAGGTTAGTTTTGAA+AGG + chr5_1:52286607-52286626 Msa0736200:intron 40.0%
GTGGGCATGCAATAATAAAG+TGG - chr5_1:52287060-52287079 None:intergenic 40.0%
ATCATCCCATTGCATTAGGT+GGG - chr5_1:52287078-52287097 None:intergenic 40.0%
TGCACATATTCTATCGAACG+CGG + chr5_1:52287325-52287344 Msa0736200:CDS 40.0%
! ATGAATGGGTTCAGTTCAAC+TGG + chr5_1:52287552-52287571 Msa0736200:three_prime_UTR 40.0%
! TGAATGGGTTCAGTTCAACT+GGG + chr5_1:52287553-52287572 Msa0736200:three_prime_UTR 40.0%
!! CAGTATATTTTGGGAATGGC+AGG + chr5_1:52287592-52287611 Msa0736200:three_prime_UTR 40.0%
! CGTACCGAATTCTTTAAGAG+TGG - chr5_1:52287680-52287699 None:intergenic 40.0%
AAAGAATTCGGTACGCAATG+GGG + chr5_1:52287685-52287704 Msa0736200:three_prime_UTR 40.0%
!!! TGTTTTGGAACAAGGGTTGG+AGG - chr5_1:52284860-52284879 None:intergenic 45.0%
ATCGAAAACTGTTATGCCGC+GGG + chr5_1:52285288-52285307 Msa0736200:intron 45.0%
TCATGTCGTGATTGAGGTCA+AGG + chr5_1:52285457-52285476 Msa0736200:intron 45.0%
GTCAATTGAAGGAGACGACT+GGG + chr5_1:52286527-52286546 Msa0736200:CDS 45.0%
TCAATTGAAGGAGACGACTG+GGG + chr5_1:52286528-52286547 Msa0736200:CDS 45.0%
! GGCATATCCACATTCTAAGG+CGG - chr5_1:52286562-52286581 None:intergenic 45.0%
GTAGTGTACTTCAGTAGCAC+AGG - chr5_1:52286583-52286602 None:intergenic 45.0%
GCTACTGAAGTACACTACTC+AGG + chr5_1:52286585-52286604 Msa0736200:CDS 45.0%
AAGAGTAGAGAAGGGAGCAA+AGG - chr5_1:52286651-52286670 None:intergenic 45.0%
CGGATGCAAAGAGTAGAGAA+GGG - chr5_1:52286659-52286678 None:intergenic 45.0%
TCGGATGCAAAGAGTAGAGA+AGG - chr5_1:52286660-52286679 None:intergenic 45.0%
CATCATCCCATTGCATTAGG+TGG - chr5_1:52287079-52287098 None:intergenic 45.0%
GCAAACATGCACATGTCTGT+CGG - chr5_1:52287220-52287239 None:intergenic 45.0%
CGTTGTAAAACAGCAAGCCA+GGG - chr5_1:52287299-52287318 None:intergenic 45.0%
TCGTTGTAAAACAGCAAGCC+AGG - chr5_1:52287300-52287319 None:intergenic 45.0%
AGCATCCAGATTGTCGACAT+GGG - chr5_1:52287376-52287395 None:intergenic 45.0%
CAATCTGGATGCTGTTGTGA+AGG + chr5_1:52287383-52287402 Msa0736200:CDS 45.0%
TTCACTAGCTCGTGCATATG+TGG - chr5_1:52287426-52287445 None:intergenic 45.0%
ACATATGCACGAGCTAGTGA+AGG + chr5_1:52287425-52287444 Msa0736200:three_prime_UTR 45.0%
CAGGTCTGATCTTCACTACA+GGG + chr5_1:52287476-52287495 Msa0736200:three_prime_UTR 45.0%
AATTGCGAACCGTGTGACTA+TGG - chr5_1:52287619-52287638 None:intergenic 45.0%
ACACGGTTCGCAATTCACTT+CGG + chr5_1:52287624-52287643 Msa0736200:three_prime_UTR 45.0%
TTCGGTACGTGGTACGAATT+CGG + chr5_1:52287642-52287661 Msa0736200:three_prime_UTR 45.0%
TATACAGAGTCGGTACGCTT+GGG + chr5_1:52287708-52287727 Msa0736200:three_prime_UTR 45.0%
!!! AATTTTATTATTTTTATTGA+TGG - chr5_1:52287825-52287844 None:intergenic 5.0%
! TTTGGAACAAGGGTTGGAGG+AGG - chr5_1:52284857-52284876 None:intergenic 50.0%
! CTTGATCTTGCTGCTTCTGC+TGG + chr5_1:52284979-52284998 Msa0736200:CDS 50.0%
GGCTGCATGTGGGGAATTTA+AGG + chr5_1:52285090-52285109 Msa0736200:intron 50.0%
CGTCAATTGAAGGAGACGAC+TGG + chr5_1:52286526-52286545 Msa0736200:CDS 50.0%
TCAGTCTCCGCCTTAGAATG+TGG + chr5_1:52286552-52286571 Msa0736200:CDS 50.0%
AGTACACTACTCAGGGTTGC+AGG + chr5_1:52286593-52286612 Msa0736200:CDS 50.0%
TGCATGCCCACCTAATGCAA+TGG + chr5_1:52287069-52287088 Msa0736200:CDS 50.0%
GCATGCCCACCTAATGCAAT+GGG + chr5_1:52287070-52287089 Msa0736200:CDS 50.0%
AGCAAGCCAGGGCTAAAGAA+GGG - chr5_1:52287288-52287307 None:intergenic 50.0%
GCATCCAGATTGTCGACATG+GGG - chr5_1:52287375-52287394 None:intergenic 50.0%
CAGCATCCAGATTGTCGACA+TGG - chr5_1:52287377-52287396 None:intergenic 50.0%
CCTGTAGTGAAGATCAGACC+TGG - chr5_1:52287478-52287497 None:intergenic 50.0%
CCAGGTCTGATCTTCACTAC+AGG + chr5_1:52287475-52287494 Msa0736200:three_prime_UTR 50.0%
GCAGCACTGTCGGAAAAGAA+AGG + chr5_1:52287504-52287523 Msa0736200:three_prime_UTR 50.0%
ATGGGTTCAGTTCAACTGGG+AGG + chr5_1:52287556-52287575 Msa0736200:three_prime_UTR 50.0%
TGGGTTCAGTTCAACTGGGA+GGG + chr5_1:52287557-52287576 Msa0736200:three_prime_UTR 50.0%
ATGGCAGGACCATAGTCACA+CGG + chr5_1:52287607-52287626 Msa0736200:three_prime_UTR 50.0%
TCGCAATTCACTTCGGTACG+TGG + chr5_1:52287631-52287650 Msa0736200:three_prime_UTR 50.0%
TCGGTACGTGGTACGAATTC+GGG + chr5_1:52287643-52287662 Msa0736200:three_prime_UTR 50.0%
GTGGTACGAATTCGGGTATG+CGG + chr5_1:52287650-52287669 Msa0736200:three_prime_UTR 50.0%
CGCAATGGGGTATACAGAGT+CGG + chr5_1:52287698-52287717 Msa0736200:three_prime_UTR 50.0%
GTATACAGAGTCGGTACGCT+TGG + chr5_1:52287707-52287726 Msa0736200:three_prime_UTR 50.0%
! CTTGCTGCTTCTGCTGGAAG+TGG + chr5_1:52284985-52285004 Msa0736200:CDS 55.0%
TTATGCCGCGGGCCAAATTG+CGG + chr5_1:52285299-52285318 Msa0736200:intron 55.0%
!! CAGTAGCTCTGGCCTGTGTA+TGG + chr5_1:52285969-52285988 Msa0736200:intron 55.0%
ACGCTTCCCTTCTTTAGCCC+TGG + chr5_1:52287279-52287298 Msa0736200:intron 55.0%
CAGCAAGCCAGGGCTAAAGA+AGG - chr5_1:52287289-52287308 None:intergenic 55.0%
TGCACCCCATGTCGACAATC+TGG + chr5_1:52287368-52287387 Msa0736200:CDS 55.0%
CAGGGAAAGAGCAGCACTGT+CGG + chr5_1:52287494-52287513 Msa0736200:three_prime_UTR 55.0%
CAAGACCGCAATTTGGCCCG+CGG - chr5_1:52285307-52285326 None:intergenic 60.0%
CGCGGGCCAAATTGCGGTCT+TGG + chr5_1:52285305-52285324 Msa0736200:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 52284750 52287969 52284750 ID=Msa0736200;Name=Msa0736200
chr5_1 mRNA 52284750 52287969 52284750 ID=Msa0736200-mRNA-1;Parent=Msa0736200;Name=Msa0736200-mRNA-1;_AED=0.31;_eAED=0.35;_QI=0|0.5|0.4|1|0.5|0.4|5|550|185
chr5_1 exon 52284750 52284883 52284750 ID=Msa0736200-mRNA-1:exon:14747;Parent=Msa0736200-mRNA-1
chr5_1 exon 52284957 52285016 52284957 ID=Msa0736200-mRNA-1:exon:14748;Parent=Msa0736200-mRNA-1
chr5_1 exon 52286473 52286614 52286473 ID=Msa0736200-mRNA-1:exon:14749;Parent=Msa0736200-mRNA-1
chr5_1 exon 52287047 52287144 52287047 ID=Msa0736200-mRNA-1:exon:14750;Parent=Msa0736200-mRNA-1
chr5_1 exon 52287296 52287969 52287296 ID=Msa0736200-mRNA-1:exon:14751;Parent=Msa0736200-mRNA-1
chr5_1 CDS 52284750 52284883 52284750 ID=Msa0736200-mRNA-1:cds;Parent=Msa0736200-mRNA-1
chr5_1 CDS 52284957 52285016 52284957 ID=Msa0736200-mRNA-1:cds;Parent=Msa0736200-mRNA-1
chr5_1 CDS 52286473 52286614 52286473 ID=Msa0736200-mRNA-1:cds;Parent=Msa0736200-mRNA-1
chr5_1 CDS 52287047 52287144 52287047 ID=Msa0736200-mRNA-1:cds;Parent=Msa0736200-mRNA-1
chr5_1 CDS 52287296 52287419 52287296 ID=Msa0736200-mRNA-1:cds;Parent=Msa0736200-mRNA-1
chr5_1 three_prime_UTR 52287420 52287969 52287420 ID=Msa0736200-mRNA-1:three_prime_utr;Parent=Msa0736200-mRNA-1
Gene Sequence

>Msa0736200

ATGGAAGCAGTAAAAATGTCTGGAAGCAATCGTGAAAGTCAAAACTCGTCTTCGAATCTCCACCATCATTCTTCATCATTATCATTACGCTGTGTTAGTATCTTCCTCCTCCAACCCTTGTTCCAAAACAATTTTGTTATCATTTTGTGGAATAGTCTTGATCTTGCTGCTTCTGCTGGAAGTGGAAATAATGAGATTTCAACAAAGTTGTGGTCTTGCAACTTCAACTCACAGCTTGCAAATGCTTCGTCAATTGAAGGAGACGACTGGGGTTCAGTCTCCGCCTTAGAATGTGGATATGCCTGTGCTACTGAAGTACACTACTCAGGGTTGCAGATATTCTTTACCACTTTATTATTGCATGCCCACCTAATGCAATGGGATGATGATAATTTGGTTGAGCAAGCTGTTAATAAATGCAATGAGATTTGGGACCCTGGCTTGCTGTTTTACAACGAATTACTGCACATATTCTATCGAACGCGGCTATGTGATTACATGAATGCTGCACCCCATGTCGACAATCTGGATGCTGTTGTGAAGGCTGAAAAGAAATAA
Protein sequence

>Msa0736200

MEAVKMSGSNRESQNSSSNLHHHSSSLSLRCVSIFLLQPLFQNNFVIILWNSLDLAASAGSGNNEISTKLWSCNFNSQLANASSIEGDDWGSVSALECGYACATEVHYSGLQIFFTTLLLHAHLMQWDDDNLVEQAVNKCNEIWDPGLLFYNELLHIFYRTRLCDYMNAAPHVDNLDAVVKAEKK*