AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0788410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0788410 MtrunA17_Chr5g0447491 100.000 130 0 0 1 130 1 130 1.18e-92 263
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0788410 AT5G49410.1 69.307 101 31 0 3 103 18 118 2.55e-51 159
Msa0788410 AT5G49410.2 69.307 101 31 0 3 103 18 118 2.55e-51 159
Msa0788410 AT1G73940.1 37.069 116 64 2 11 126 43 149 3.73e-23 88.6

Find 48 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTGTAGCTTCTCTAAA+TGG 0.231985 5_2:+74994084 None:intergenic
TCCTTCTGAGTCTTGGCTTA+TGG 0.232824 5_2:+74994396 None:intergenic
AATTAGAGTAGTGAGAAATT+AGG 0.247656 5_2:-74995877 Msa0788410:five_prime_UTR
ATTAGAGTAGTGAGAAATTA+GGG 0.258869 5_2:-74995876 Msa0788410:five_prime_UTR
TGTTATTCATTAGTGTAAAC+TGG 0.347893 5_2:-74994231 Msa0788410:three_prime_UTR
GTTATTGTTTGTCTCTGTTC+CGG 0.354082 5_2:+74995765 None:intergenic
TTATGCAATGGGAAGAATAA+TGG 0.354896 5_2:-74994360 Msa0788410:three_prime_UTR
CCAATGAAGAATCTGAATAC+TGG 0.366030 5_2:-74994311 Msa0788410:three_prime_UTR
TGGCCCATCCTTCTGAGTCT+TGG 0.377407 5_2:+74994389 None:intergenic
AGCTCTCTTTGATATTGTTA+AGG 0.392484 5_2:-74995739 Msa0788410:CDS
GGAACAGAAGCAACACAATC+AGG 0.417769 5_2:+74994256 None:intergenic
CCAGTATTCAGATTCTTCAT+TGG 0.421543 5_2:+74994311 None:intergenic
CTATCACCGTTTCTAATACC+TGG 0.429853 5_2:-74995707 Msa0788410:CDS
TCATTGTTAGCATTAGTTAG+TGG 0.441886 5_2:-74994160 Msa0788410:three_prime_UTR
AGCGTTACCGCTCCCAGCTT+CGG 0.442104 5_2:+74995798 None:intergenic
AGTTTGAGTGGTACCTGGTT+TGG 0.446620 5_2:+74994503 None:intergenic
GAAGAAACTGAAGCCGAAGC+TGG 0.455351 5_2:-74995811 Msa0788410:CDS
ATTTAACACAACCAACCATA+TGG 0.462311 5_2:+74994287 None:intergenic
AATTACCTTGACACGTTCCC+AGG 0.464678 5_2:+74995689 None:intergenic
AAAGCATTCCTTCAAAGAAA+AGG 0.466561 5_2:-74994452 Msa0788410:CDS
AGCACTATGTATTGATGCCT+TGG 0.468698 5_2:-74994196 Msa0788410:three_prime_UTR
CGTCTTGTTTGCAACCATGT+TGG 0.476670 5_2:-74994561 Msa0788410:CDS
TTCCCATTGCATAAAACAGC+TGG 0.482960 5_2:+74994369 None:intergenic
AATCTGAATACTGGGCCATA+TGG 0.489508 5_2:-74994302 Msa0788410:three_prime_UTR
GTTGTAGTTTGAGTGGTACC+TGG 0.498914 5_2:+74994498 None:intergenic
CAATGAAGAATCTGAATACT+GGG 0.500380 5_2:-74994310 Msa0788410:three_prime_UTR
GGTTTGAGAAGAGAAATGTT+TGG 0.500931 5_2:-74995855 Msa0788410:exon
CACAATTCAGAGCTTAACTT+AGG 0.505427 5_2:-74996038 Msa0788410:intron
ACCACTTGGTTTACCAAACC+AGG 0.536017 5_2:-74994516 Msa0788410:CDS
TTTGATATTGTTAAGGAACA+TGG 0.539683 5_2:-74995732 Msa0788410:CDS
TATCACCGTTTCTAATACCT+GGG 0.548790 5_2:-74995706 Msa0788410:CDS
AACAGTTTCTCTATACCACT+TGG 0.548939 5_2:-74994530 Msa0788410:CDS
AAGAAACTGAAGCCGAAGCT+GGG 0.553771 5_2:-74995810 Msa0788410:CDS
CAGGTATTAGAAACGGTGAT+AGG 0.556544 5_2:+74995708 None:intergenic
AAGCCAAGACTCAGAAGGAT+GGG 0.568171 5_2:-74994392 Msa0788410:three_prime_UTR
TAATACCTGGGAACGTGTCA+AGG 0.569738 5_2:-74995694 Msa0788410:intron
ACGTTCCCAGGTATTAGAAA+CGG 0.576633 5_2:+74995701 None:intergenic
TAAGCCAAGACTCAGAAGGA+TGG 0.579557 5_2:-74994393 Msa0788410:three_prime_UTR
CGTCGTCGTTGTAGTTTGAG+TGG 0.581233 5_2:+74994491 None:intergenic
ATGAGATAAAACAATTGCCA+AGG 0.592832 5_2:+74994179 None:intergenic
TTTACACTAATGAATAACAT+AGG 0.594165 5_2:+74994235 None:intergenic
TGAATACTGGGCCATATGGT+TGG 0.598685 5_2:-74994298 Msa0788410:three_prime_UTR
ACTGAAGCCGAAGCTGGGAG+CGG 0.599839 5_2:-74995805 Msa0788410:CDS
GCCATAAGCCAAGACTCAGA+AGG 0.628117 5_2:-74994397 Msa0788410:three_prime_UTR
GGTAACGCTGACAACGCCGC+CGG 0.632194 5_2:-74995784 Msa0788410:CDS
ACTGTTTATGAGCTCCAACA+TGG 0.634162 5_2:+74994547 None:intergenic
ATTGTTTGTCTCTGTTCCGG+CGG 0.660646 5_2:+74995768 None:intergenic
ACCTGGTTTGGTAAACCAAG+TGG 0.666171 5_2:+74994515 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTTCATAGTAATAATT+AGG + chr5_2:74994143-74994162 None:intergenic 15.0%
!!! TGCTATTTTAGAAATTAATT+TGG - chr5_2:74996023-74996042 Msa0788410:intron 15.0%
!! TTCATTTCATAGTAATAATT+AGG + chr5_2:74994143-74994162 None:intergenic 15.0%
!!! TGCTATTTTAGAAATTAATT+TGG - chr5_2:74996023-74996042 Msa0788410:intron 15.0%
!! TTAAAAACAAACATCATGTA+TGG + chr5_2:74994496-74994515 None:intergenic 20.0%
!! TTTACACTAATGAATAACAT+AGG + chr5_2:74995898-74995917 None:intergenic 20.0%
!! AAATAAGAAAAATCATCAAG+AGG - chr5_2:74996063-74996082 Msa0788410:five_prime_UTR 20.0%
!! AATAAGAAAAATCATCAAGA+GGG - chr5_2:74996064-74996083 Msa0788410:five_prime_UTR 20.0%
!! TTAAAAACAAACATCATGTA+TGG + chr5_2:74994496-74994515 None:intergenic 20.0%
!! TTTACACTAATGAATAACAT+AGG + chr5_2:74995898-74995917 None:intergenic 20.0%
!! AAATAAGAAAAATCATCAAG+AGG - chr5_2:74996063-74996082 Msa0788410:five_prime_UTR 20.0%
!! AATAAGAAAAATCATCAAGA+GGG - chr5_2:74996064-74996083 Msa0788410:five_prime_UTR 20.0%
! AATTAGAGTAGTGAGAAATT+AGG - chr5_2:74994253-74994272 Msa0788410:three_prime_UTR 25.0%
! ATTAGAGTAGTGAGAAATTA+GGG - chr5_2:74994254-74994273 Msa0788410:three_prime_UTR 25.0%
! TTTGATATTGTTAAGGAACA+TGG - chr5_2:74994398-74994417 Msa0788410:three_prime_UTR 25.0%
!!! ATTGTTTCTGCATTATGTTT+TGG - chr5_2:74994530-74994549 Msa0788410:CDS 25.0%
!!! TGTTTTGAAATAGTGATGTA+TGG - chr5_2:74994588-74994607 Msa0788410:CDS 25.0%
! CATACTACATTAGATGTAAT+AGG - chr5_2:74994841-74994860 Msa0788410:intron 25.0%
! ACATAAGAATTTACATGGAT+CGG - chr5_2:74994866-74994885 Msa0788410:intron 25.0%
! TAAAACAGTGAAAAAATTGC+TGG + chr5_2:74994908-74994927 None:intergenic 25.0%
! AAAATTGTTACTCTGTTGAA+GGG - chr5_2:74995202-74995221 Msa0788410:intron 25.0%
!!! ATTGTTTTGTTTACTTGTGA+TGG - chr5_2:74995385-74995404 Msa0788410:intron 25.0%
! ACAGATATGTAACATTCATA+AGG + chr5_2:74995414-74995433 None:intergenic 25.0%
! TGTTATTCATTAGTGTAAAC+TGG - chr5_2:74995899-74995918 Msa0788410:five_prime_UTR 25.0%
! TTATTTGTAGCTTCTCTAAA+TGG + chr5_2:74996049-74996068 None:intergenic 25.0%
! AATTAGAGTAGTGAGAAATT+AGG - chr5_2:74994253-74994272 Msa0788410:three_prime_UTR 25.0%
! ATTAGAGTAGTGAGAAATTA+GGG - chr5_2:74994254-74994273 Msa0788410:three_prime_UTR 25.0%
! TTTGATATTGTTAAGGAACA+TGG - chr5_2:74994398-74994417 Msa0788410:three_prime_UTR 25.0%
!!! ATTGTTTCTGCATTATGTTT+TGG - chr5_2:74994530-74994549 Msa0788410:CDS 25.0%
!!! TGTTTTGAAATAGTGATGTA+TGG - chr5_2:74994588-74994607 Msa0788410:CDS 25.0%
! CATACTACATTAGATGTAAT+AGG - chr5_2:74994841-74994860 Msa0788410:intron 25.0%
! ACATAAGAATTTACATGGAT+CGG - chr5_2:74994866-74994885 Msa0788410:intron 25.0%
! TAAAACAGTGAAAAAATTGC+TGG + chr5_2:74994908-74994927 None:intergenic 25.0%
! AAAATTGTTACTCTGTTGAA+GGG - chr5_2:74995202-74995221 Msa0788410:intron 25.0%
!!! ATTGTTTTGTTTACTTGTGA+TGG - chr5_2:74995385-74995404 Msa0788410:intron 25.0%
! ACAGATATGTAACATTCATA+AGG + chr5_2:74995414-74995433 None:intergenic 25.0%
! TGTTATTCATTAGTGTAAAC+TGG - chr5_2:74995899-74995918 Msa0788410:five_prime_UTR 25.0%
! TTATTTGTAGCTTCTCTAAA+TGG + chr5_2:74996049-74996068 None:intergenic 25.0%
AGCTCTCTTTGATATTGTTA+AGG - chr5_2:74994391-74994410 Msa0788410:three_prime_UTR 30.0%
AGGACACATAAGAATTTACA+TGG - chr5_2:74994861-74994880 Msa0788410:intron 30.0%
TAGAATCTTGAAAACTTGTG+TGG - chr5_2:74994948-74994967 Msa0788410:intron 30.0%
GATTACTACTCAAGTTTACA+GGG + chr5_2:74994976-74994995 None:intergenic 30.0%
ATACAAACAAAATCACGTGA+AGG + chr5_2:74995101-74995120 None:intergenic 30.0%
!! ACGTGATTTTGTTTGTATAG+TGG - chr5_2:74995103-74995122 Msa0788410:intron 30.0%
!! TGATTTTGTTTGTATAGTGG+AGG - chr5_2:74995106-74995125 Msa0788410:intron 30.0%
GAAAATTGTTACTCTGTTGA+AGG - chr5_2:74995201-74995220 Msa0788410:intron 30.0%
TTACATATCTGTGCATCTAT+TGG - chr5_2:74995422-74995441 Msa0788410:intron 30.0%
AACTTCCTGGAAAAAATTGA+AGG + chr5_2:74995477-74995496 None:intergenic 30.0%
!!! TTCAATTTTTTCCAGGAAGT+TGG - chr5_2:74995476-74995495 Msa0788410:intron 30.0%
!!! ACATGAAAGTAGTTTTGAGA+TGG - chr5_2:74995525-74995544 Msa0788410:intron 30.0%
! AAAGCATTCCTTCAAAGAAA+AGG - chr5_2:74995678-74995697 Msa0788410:intron 30.0%
TTATGCAATGGGAAGAATAA+TGG - chr5_2:74995770-74995789 Msa0788410:CDS 30.0%
CAATGAAGAATCTGAATACT+GGG - chr5_2:74995820-74995839 Msa0788410:CDS 30.0%
ATTTAACACAACCAACCATA+TGG + chr5_2:74995846-74995865 None:intergenic 30.0%
ATGAGATAAAACAATTGCCA+AGG + chr5_2:74995954-74995973 None:intergenic 30.0%
TCATTGTTAGCATTAGTTAG+TGG - chr5_2:74995970-74995989 Msa0788410:intron 30.0%
AGCTCTCTTTGATATTGTTA+AGG - chr5_2:74994391-74994410 Msa0788410:three_prime_UTR 30.0%
AGGACACATAAGAATTTACA+TGG - chr5_2:74994861-74994880 Msa0788410:intron 30.0%
TAGAATCTTGAAAACTTGTG+TGG - chr5_2:74994948-74994967 Msa0788410:intron 30.0%
GATTACTACTCAAGTTTACA+GGG + chr5_2:74994976-74994995 None:intergenic 30.0%
ATACAAACAAAATCACGTGA+AGG + chr5_2:74995101-74995120 None:intergenic 30.0%
!! ACGTGATTTTGTTTGTATAG+TGG - chr5_2:74995103-74995122 Msa0788410:intron 30.0%
!! TGATTTTGTTTGTATAGTGG+AGG - chr5_2:74995106-74995125 Msa0788410:intron 30.0%
GAAAATTGTTACTCTGTTGA+AGG - chr5_2:74995201-74995220 Msa0788410:intron 30.0%
TTACATATCTGTGCATCTAT+TGG - chr5_2:74995422-74995441 Msa0788410:intron 30.0%
AACTTCCTGGAAAAAATTGA+AGG + chr5_2:74995477-74995496 None:intergenic 30.0%
!!! TTCAATTTTTTCCAGGAAGT+TGG - chr5_2:74995476-74995495 Msa0788410:intron 30.0%
!!! ACATGAAAGTAGTTTTGAGA+TGG - chr5_2:74995525-74995544 Msa0788410:intron 30.0%
! AAAGCATTCCTTCAAAGAAA+AGG - chr5_2:74995678-74995697 Msa0788410:intron 30.0%
TTATGCAATGGGAAGAATAA+TGG - chr5_2:74995770-74995789 Msa0788410:CDS 30.0%
CAATGAAGAATCTGAATACT+GGG - chr5_2:74995820-74995839 Msa0788410:CDS 30.0%
ATTTAACACAACCAACCATA+TGG + chr5_2:74995846-74995865 None:intergenic 30.0%
ATGAGATAAAACAATTGCCA+AGG + chr5_2:74995954-74995973 None:intergenic 30.0%
TCATTGTTAGCATTAGTTAG+TGG - chr5_2:74995970-74995989 Msa0788410:intron 30.0%
CACAATTCAGAGCTTAACTT+AGG - chr5_2:74994092-74994111 Msa0788410:three_prime_UTR 35.0%
GGTTTGAGAAGAGAAATGTT+TGG - chr5_2:74994275-74994294 Msa0788410:three_prime_UTR 35.0%
GTTATTGTTTGTCTCTGTTC+CGG + chr5_2:74994368-74994387 None:intergenic 35.0%
TATCACCGTTTCTAATACCT+GGG - chr5_2:74994424-74994443 Msa0788410:exon 35.0%
ATACATATCTACAGTGCTAG+TGG - chr5_2:74994751-74994770 Msa0788410:intron 35.0%
TACATATCTACAGTGCTAGT+GGG - chr5_2:74994752-74994771 Msa0788410:intron 35.0%
CATCTTCTCAGCATTAATTG+CGG + chr5_2:74994793-74994812 None:intergenic 35.0%
GCAATTAATGCTGAGAAGAT+GGG - chr5_2:74994792-74994811 Msa0788410:intron 35.0%
GGATTACTACTCAAGTTTAC+AGG + chr5_2:74994977-74994996 None:intergenic 35.0%
TAATCCACTCAAGGATTTCA+AGG - chr5_2:74994991-74995010 Msa0788410:intron 35.0%
TATTCCTTGAAATCCTTGAG+TGG + chr5_2:74994998-74995017 None:intergenic 35.0%
CCAGGATAAATGTGCAAATA+TGG + chr5_2:74995064-74995083 None:intergenic 35.0%
CCATATTTGCACATTTATCC+TGG - chr5_2:74995061-74995080 Msa0788410:intron 35.0%
AAGGATCAAAACTCAAATCC+AGG + chr5_2:74995082-74995101 None:intergenic 35.0%
!! TTTTGTTTGTATAGTGGAGG+TGG - chr5_2:74995109-74995128 Msa0788410:intron 35.0%
CTGGAAAAAATTGAAGGTCA+TGG + chr5_2:74995471-74995490 None:intergenic 35.0%
!!! CATGACCTTCAATTTTTTCC+AGG - chr5_2:74995469-74995488 Msa0788410:intron 35.0%
AACAGTTTCTCTATACCACT+TGG - chr5_2:74995600-74995619 Msa0788410:intron 35.0%
CCAGTATTCAGATTCTTCAT+TGG + chr5_2:74995822-74995841 None:intergenic 35.0%
CCAATGAAGAATCTGAATAC+TGG - chr5_2:74995819-74995838 Msa0788410:CDS 35.0%
AAATCATCAAGAGGGATTTC+AGG - chr5_2:74996072-74996091 Msa0788410:five_prime_UTR 35.0%
CACAATTCAGAGCTTAACTT+AGG - chr5_2:74994092-74994111 Msa0788410:three_prime_UTR 35.0%
GGTTTGAGAAGAGAAATGTT+TGG - chr5_2:74994275-74994294 Msa0788410:three_prime_UTR 35.0%
GTTATTGTTTGTCTCTGTTC+CGG + chr5_2:74994368-74994387 None:intergenic 35.0%
TATCACCGTTTCTAATACCT+GGG - chr5_2:74994424-74994443 Msa0788410:exon 35.0%
ATACATATCTACAGTGCTAG+TGG - chr5_2:74994751-74994770 Msa0788410:intron 35.0%
TACATATCTACAGTGCTAGT+GGG - chr5_2:74994752-74994771 Msa0788410:intron 35.0%
CATCTTCTCAGCATTAATTG+CGG + chr5_2:74994793-74994812 None:intergenic 35.0%
GCAATTAATGCTGAGAAGAT+GGG - chr5_2:74994792-74994811 Msa0788410:intron 35.0%
GGATTACTACTCAAGTTTAC+AGG + chr5_2:74994977-74994996 None:intergenic 35.0%
TAATCCACTCAAGGATTTCA+AGG - chr5_2:74994991-74995010 Msa0788410:intron 35.0%
TATTCCTTGAAATCCTTGAG+TGG + chr5_2:74994998-74995017 None:intergenic 35.0%
CCAGGATAAATGTGCAAATA+TGG + chr5_2:74995064-74995083 None:intergenic 35.0%
CCATATTTGCACATTTATCC+TGG - chr5_2:74995061-74995080 Msa0788410:intron 35.0%
AAGGATCAAAACTCAAATCC+AGG + chr5_2:74995082-74995101 None:intergenic 35.0%
!! TTTTGTTTGTATAGTGGAGG+TGG - chr5_2:74995109-74995128 Msa0788410:intron 35.0%
CTGGAAAAAATTGAAGGTCA+TGG + chr5_2:74995471-74995490 None:intergenic 35.0%
!!! CATGACCTTCAATTTTTTCC+AGG - chr5_2:74995469-74995488 Msa0788410:intron 35.0%
AACAGTTTCTCTATACCACT+TGG - chr5_2:74995600-74995619 Msa0788410:intron 35.0%
CCAGTATTCAGATTCTTCAT+TGG + chr5_2:74995822-74995841 None:intergenic 35.0%
CCAATGAAGAATCTGAATAC+TGG - chr5_2:74995819-74995838 Msa0788410:CDS 35.0%
CAGGTATTAGAAACGGTGAT+AGG + chr5_2:74994425-74994444 None:intergenic 40.0%
CTATCACCGTTTCTAATACC+TGG - chr5_2:74994423-74994442 Msa0788410:exon 40.0%
ACGTTCCCAGGTATTAGAAA+CGG + chr5_2:74994432-74994451 None:intergenic 40.0%
AATAATTCGCTATTGTGCCC+TGG - chr5_2:74994630-74994649 Msa0788410:CDS 40.0%
CGCAATTAATGCTGAGAAGA+TGG - chr5_2:74994791-74994810 Msa0788410:intron 40.0%
TTTACATGGATCGGAGTCTA+AGG - chr5_2:74994875-74994894 Msa0788410:intron 40.0%
CTTGAGTAGTAATCCACTCA+AGG - chr5_2:74994982-74995001 Msa0788410:intron 40.0%
GATCTTTCAATCCAACTTCC+TGG + chr5_2:74995490-74995509 None:intergenic 40.0%
ACTGTTTATGAGCTCCAACA+TGG + chr5_2:74995586-74995605 None:intergenic 40.0%
TTCCCATTGCATAAAACAGC+TGG + chr5_2:74995764-74995783 None:intergenic 40.0%
AATCTGAATACTGGGCCATA+TGG - chr5_2:74995828-74995847 Msa0788410:CDS 40.0%
! AGCACTATGTATTGATGCCT+TGG - chr5_2:74995934-74995953 Msa0788410:intron 40.0%
CAGGTATTAGAAACGGTGAT+AGG + chr5_2:74994425-74994444 None:intergenic 40.0%
CTATCACCGTTTCTAATACC+TGG - chr5_2:74994423-74994442 Msa0788410:exon 40.0%
ACGTTCCCAGGTATTAGAAA+CGG + chr5_2:74994432-74994451 None:intergenic 40.0%
AATAATTCGCTATTGTGCCC+TGG - chr5_2:74994630-74994649 Msa0788410:CDS 40.0%
CGCAATTAATGCTGAGAAGA+TGG - chr5_2:74994791-74994810 Msa0788410:intron 40.0%
TTTACATGGATCGGAGTCTA+AGG - chr5_2:74994875-74994894 Msa0788410:intron 40.0%
CTTGAGTAGTAATCCACTCA+AGG - chr5_2:74994982-74995001 Msa0788410:intron 40.0%
GATCTTTCAATCCAACTTCC+TGG + chr5_2:74995490-74995509 None:intergenic 40.0%
ACTGTTTATGAGCTCCAACA+TGG + chr5_2:74995586-74995605 None:intergenic 40.0%
TTCCCATTGCATAAAACAGC+TGG + chr5_2:74995764-74995783 None:intergenic 40.0%
AATCTGAATACTGGGCCATA+TGG - chr5_2:74995828-74995847 Msa0788410:CDS 40.0%
! AGCACTATGTATTGATGCCT+TGG - chr5_2:74995934-74995953 Msa0788410:intron 40.0%
AAGAAACTGAAGCCGAAGCT+GGG - chr5_2:74994320-74994339 Msa0788410:three_prime_UTR 45.0%
ATTGTTTGTCTCTGTTCCGG+CGG + chr5_2:74994365-74994384 None:intergenic 45.0%
TAATACCTGGGAACGTGTCA+AGG - chr5_2:74994436-74994455 Msa0788410:exon 45.0%
AATTACCTTGACACGTTCCC+AGG + chr5_2:74994444-74994463 None:intergenic 45.0%
CTGTCCTAAATAGCGTACCA+GGG + chr5_2:74994650-74994669 None:intergenic 45.0%
ACTGTCCTAAATAGCGTACC+AGG + chr5_2:74994651-74994670 None:intergenic 45.0%
AGATGGGTGTGTTGGATGTT+TGG - chr5_2:74994808-74994827 Msa0788410:intron 45.0%
! TGTTTGTATAGTGGAGGTGG+AGG - chr5_2:74995112-74995131 Msa0788410:intron 45.0%
! GTTTGTATAGTGGAGGTGGA+GGG - chr5_2:74995113-74995132 Msa0788410:intron 45.0%
!! GAGGGAAAGTGACTTGTTCA+AGG - chr5_2:74995131-74995150 Msa0788410:intron 45.0%
!! CGTCTTGTTTGCAACCATGT+TGG - chr5_2:74995569-74995588 Msa0788410:intron 45.0%
ACCACTTGGTTTACCAAACC+AGG - chr5_2:74995614-74995633 Msa0788410:intron 45.0%
!! ACCTGGTTTGGTAAACCAAG+TGG + chr5_2:74995618-74995637 None:intergenic 45.0%
AGTTTGAGTGGTACCTGGTT+TGG + chr5_2:74995630-74995649 None:intergenic 45.0%
GTTGTAGTTTGAGTGGTACC+TGG + chr5_2:74995635-74995654 None:intergenic 45.0%
!!! CGTGCTAGCCTTTTCTTTGA+AGG + chr5_2:74995689-74995708 None:intergenic 45.0%
TCCTTCTGAGTCTTGGCTTA+TGG + chr5_2:74995737-74995756 None:intergenic 45.0%
TAAGCCAAGACTCAGAAGGA+TGG - chr5_2:74995737-74995756 Msa0788410:CDS 45.0%
AAGCCAAGACTCAGAAGGAT+GGG - chr5_2:74995738-74995757 Msa0788410:CDS 45.0%
!! GGCCAGCTGTTTTATGCAAT+GGG - chr5_2:74995759-74995778 Msa0788410:CDS 45.0%
TGAATACTGGGCCATATGGT+TGG - chr5_2:74995832-74995851 Msa0788410:CDS 45.0%
GGAACAGAAGCAACACAATC+AGG + chr5_2:74995877-74995896 None:intergenic 45.0%
AAGAAACTGAAGCCGAAGCT+GGG - chr5_2:74994320-74994339 Msa0788410:three_prime_UTR 45.0%
ATTGTTTGTCTCTGTTCCGG+CGG + chr5_2:74994365-74994384 None:intergenic 45.0%
TAATACCTGGGAACGTGTCA+AGG - chr5_2:74994436-74994455 Msa0788410:exon 45.0%
AATTACCTTGACACGTTCCC+AGG + chr5_2:74994444-74994463 None:intergenic 45.0%
CTGTCCTAAATAGCGTACCA+GGG + chr5_2:74994650-74994669 None:intergenic 45.0%
ACTGTCCTAAATAGCGTACC+AGG + chr5_2:74994651-74994670 None:intergenic 45.0%
AGATGGGTGTGTTGGATGTT+TGG - chr5_2:74994808-74994827 Msa0788410:intron 45.0%
! TGTTTGTATAGTGGAGGTGG+AGG - chr5_2:74995112-74995131 Msa0788410:intron 45.0%
! GTTTGTATAGTGGAGGTGGA+GGG - chr5_2:74995113-74995132 Msa0788410:intron 45.0%
!! GAGGGAAAGTGACTTGTTCA+AGG - chr5_2:74995131-74995150 Msa0788410:intron 45.0%
!! CGTCTTGTTTGCAACCATGT+TGG - chr5_2:74995569-74995588 Msa0788410:intron 45.0%
ACCACTTGGTTTACCAAACC+AGG - chr5_2:74995614-74995633 Msa0788410:intron 45.0%
!! ACCTGGTTTGGTAAACCAAG+TGG + chr5_2:74995618-74995637 None:intergenic 45.0%
AGTTTGAGTGGTACCTGGTT+TGG + chr5_2:74995630-74995649 None:intergenic 45.0%
GTTGTAGTTTGAGTGGTACC+TGG + chr5_2:74995635-74995654 None:intergenic 45.0%
!!! CGTGCTAGCCTTTTCTTTGA+AGG + chr5_2:74995689-74995708 None:intergenic 45.0%
TCCTTCTGAGTCTTGGCTTA+TGG + chr5_2:74995737-74995756 None:intergenic 45.0%
TAAGCCAAGACTCAGAAGGA+TGG - chr5_2:74995737-74995756 Msa0788410:CDS 45.0%
AAGCCAAGACTCAGAAGGAT+GGG - chr5_2:74995738-74995757 Msa0788410:CDS 45.0%
!! GGCCAGCTGTTTTATGCAAT+GGG - chr5_2:74995759-74995778 Msa0788410:CDS 45.0%
TGAATACTGGGCCATATGGT+TGG - chr5_2:74995832-74995851 Msa0788410:CDS 45.0%
GGAACAGAAGCAACACAATC+AGG + chr5_2:74995877-74995896 None:intergenic 45.0%
GAAGAAACTGAAGCCGAAGC+TGG - chr5_2:74994319-74994338 Msa0788410:three_prime_UTR 50.0%
TGTGCCCTGGTACGCTATTT+AGG - chr5_2:74994643-74994662 Msa0788410:intron 50.0%
TGCTGAGAAGATGGGTGTGT+TGG - chr5_2:74994800-74994819 Msa0788410:intron 50.0%
CGTCGTCGTTGTAGTTTGAG+TGG + chr5_2:74995642-74995661 None:intergenic 50.0%
GCCATAAGCCAAGACTCAGA+AGG - chr5_2:74995733-74995752 Msa0788410:CDS 50.0%
!! GGGCCAGCTGTTTTATGCAA+TGG - chr5_2:74995758-74995777 Msa0788410:CDS 50.0%
GAAGAAACTGAAGCCGAAGC+TGG - chr5_2:74994319-74994338 Msa0788410:three_prime_UTR 50.0%
TGTGCCCTGGTACGCTATTT+AGG - chr5_2:74994643-74994662 Msa0788410:intron 50.0%
TGCTGAGAAGATGGGTGTGT+TGG - chr5_2:74994800-74994819 Msa0788410:intron 50.0%
CGTCGTCGTTGTAGTTTGAG+TGG + chr5_2:74995642-74995661 None:intergenic 50.0%
GCCATAAGCCAAGACTCAGA+AGG - chr5_2:74995733-74995752 Msa0788410:CDS 50.0%
!! GGGCCAGCTGTTTTATGCAA+TGG - chr5_2:74995758-74995777 Msa0788410:CDS 50.0%
TGGCCCATCCTTCTGAGTCT+TGG + chr5_2:74995744-74995763 None:intergenic 55.0%
TGGCCCATCCTTCTGAGTCT+TGG + chr5_2:74995744-74995763 None:intergenic 55.0%
ACTGAAGCCGAAGCTGGGAG+CGG - chr5_2:74994325-74994344 Msa0788410:three_prime_UTR 60.0%
AGCGTTACCGCTCCCAGCTT+CGG + chr5_2:74994335-74994354 None:intergenic 60.0%
ACTGAAGCCGAAGCTGGGAG+CGG - chr5_2:74994325-74994344 Msa0788410:three_prime_UTR 60.0%
AGCGTTACCGCTCCCAGCTT+CGG + chr5_2:74994335-74994354 None:intergenic 60.0%
GGTAACGCTGACAACGCCGC+CGG - chr5_2:74994346-74994365 Msa0788410:three_prime_UTR 65.0%
GGTAACGCTGACAACGCCGC+CGG - chr5_2:74994346-74994365 Msa0788410:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 74994059 74996093 74994059 ID=Msa0788410;Name=Msa0788410
chr5_2 mRNA 74994059 74996093 74994059 ID=Msa0788410-mRNA-1;Parent=Msa0788410;Name=Msa0788410-mRNA-1;_AED=0.07;_eAED=0.07;_QI=112|1|1|1|0.5|0.33|3|378|130
chr5_2 exon 74994059 74994661 74994059 ID=Msa0788410-mRNA-1:exon:22953;Parent=Msa0788410-mRNA-1
chr5_2 exon 74995695 74995919 74995695 ID=Msa0788410-mRNA-1:exon:22952;Parent=Msa0788410-mRNA-1
chr5_2 exon 74996039 74996093 74996039 ID=Msa0788410-mRNA-1:exon:22951;Parent=Msa0788410-mRNA-1
chr5_2 five_prime_UTR 74996039 74996093 74996039 ID=Msa0788410-mRNA-1:five_prime_utr;Parent=Msa0788410-mRNA-1
chr5_2 five_prime_UTR 74995863 74995919 74995863 ID=Msa0788410-mRNA-1:five_prime_utr;Parent=Msa0788410-mRNA-1
chr5_2 CDS 74995695 74995862 74995695 ID=Msa0788410-mRNA-1:cds;Parent=Msa0788410-mRNA-1
chr5_2 CDS 74994437 74994661 74994437 ID=Msa0788410-mRNA-1:cds;Parent=Msa0788410-mRNA-1
chr5_2 three_prime_UTR 74994059 74994436 74994059 ID=Msa0788410-mRNA-1:three_prime_utr;Parent=Msa0788410-mRNA-1
Gene Sequence

>Msa0788410

ATGTTTGGAACAGCGATTAGAAATGCAGCGAAGAAACTGAAGCCGAAGCTGGGAGCGGTAACGCTGACAACGCCGCCGGAACAGAGACAAACAATAACAAGAGCTCTCTTTGATATTGTTAAGGAACATGGTCCTATCACCGTTTCTAATACCTGGGAACGTGTCAAGGAAGTTGGATTGAAAGATCTGACAAGCAAGAATCACATGAAAGTAGTTTTGAGATGGATGAGAGAGAGACAGAAGCTTCGTCTTGTTTGCAACCATGTTGGAGCTCATAAACAGTTTCTCTATACCACTTGGTTTACCAAACCAGGTACCACTCAAACTACAACGACGACGACAACAACAACAACGAAAAGCATTCCTTCAAAGAAAAGGCTAGCACGATCTTGA
Protein sequence

>Msa0788410

MFGTAIRNAAKKLKPKLGAVTLTTPPEQRQTITRALFDIVKEHGPITVSNTWERVKEVGLKDLTSKNHMKVVLRWMRERQKLRLVCNHVGAHKQFLYTTWFTKPGTTQTTTTTTTTTTKSIPSKKRLARS*