AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0826580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826580 MtrunA17_Chr5g0447491 100.000 130 0 0 1 130 1 130 1.18e-92 263
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826580 AT5G49410.1 69.307 101 31 0 3 103 18 118 2.55e-51 159
Msa0826580 AT5G49410.2 69.307 101 31 0 3 103 18 118 2.55e-51 159
Msa0826580 AT1G73940.1 37.069 116 64 2 11 126 43 149 3.73e-23 88.6

Find 48 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTGTAGCTTCTCTAAA+TGG 0.231985 5_3:-67084100 None:intergenic
TCCTTCTGAGTCTTGGCTTA+TGG 0.232824 5_3:-67083788 None:intergenic
AATTAGAGTAGTGAGAAATT+AGG 0.247656 5_3:+67082307 Msa0826580:five_prime_UTR
ATTAGAGTAGTGAGAAATTA+GGG 0.258869 5_3:+67082308 Msa0826580:five_prime_UTR
CCAATGAAGAATCTGAATAT+TGG 0.298020 5_3:+67083873 Msa0826580:three_prime_UTR
TGTTATTCATTAGTGTAAAC+TGG 0.347893 5_3:+67083953 Msa0826580:three_prime_UTR
GTTATTGTTTGTCTCTGTTC+CGG 0.354082 5_3:-67082419 None:intergenic
TTATGCAATGGGAAGAATAA+TGG 0.354896 5_3:+67083824 Msa0826580:three_prime_UTR
CAATGAAGAATCTGAATATT+GGG 0.360546 5_3:+67083874 Msa0826580:three_prime_UTR
TGGCCCATCCTTCTGAGTCT+TGG 0.377407 5_3:-67083795 None:intergenic
AGCTCTCTTTGATATTGTTA+AGG 0.392484 5_3:+67082445 Msa0826580:CDS
CCAATATTCAGATTCTTCAT+TGG 0.393372 5_3:-67083873 None:intergenic
GGAACAGAAGCAACACAATC+AGG 0.417769 5_3:-67083928 None:intergenic
CTATCACCGTTTCTAATACC+TGG 0.429853 5_3:+67082477 Msa0826580:CDS
TCATTGTTAGCATTAGTTAG+TGG 0.441886 5_3:+67084024 Msa0826580:three_prime_UTR
AGCGTTACCGCTCCCAGCTT+CGG 0.442104 5_3:-67082386 None:intergenic
AGTTTGAGTGGTACCTGGTT+TGG 0.446620 5_3:-67083681 None:intergenic
GAAGAAACTGAAGCCGAAGC+TGG 0.455351 5_3:+67082373 Msa0826580:CDS
ATTTAACACAACCAACCATA+TGG 0.462311 5_3:-67083897 None:intergenic
AATTACCTTGACACGTTCCC+AGG 0.464678 5_3:-67082495 None:intergenic
AAAGCATTCCTTCAAAGAAA+AGG 0.466561 5_3:+67083732 Msa0826580:CDS
AGCACTATGTATTGATGCCT+TGG 0.468698 5_3:+67083988 Msa0826580:three_prime_UTR
AATCTGAATATTGGGCCATA+TGG 0.469274 5_3:+67083882 Msa0826580:three_prime_UTR
CGTCTTGTTTGCAACCATGT+TGG 0.476670 5_3:+67083623 Msa0826580:CDS
TTCCCATTGCATAAAACAGC+TGG 0.482960 5_3:-67083815 None:intergenic
GTTGTAGTTTGAGTGGTACC+TGG 0.498914 5_3:-67083686 None:intergenic
GGTTTGAGAAGAGAAATGTT+TGG 0.500931 5_3:+67082329 Msa0826580:exon
CACAATTCAGAGCTTAACTT+AGG 0.505427 5_3:+67082146 Msa0826580:five_prime_UTR
ACCACTTGGTTTACCAAACC+AGG 0.536017 5_3:+67083668 Msa0826580:CDS
TTTGATATTGTTAAGGAACA+TGG 0.539683 5_3:+67082452 Msa0826580:CDS
TATCACCGTTTCTAATACCT+GGG 0.548790 5_3:+67082478 Msa0826580:CDS
AACAGTTTCTCTATACCACT+TGG 0.548939 5_3:+67083654 Msa0826580:CDS
AAGAAACTGAAGCCGAAGCT+GGG 0.553771 5_3:+67082374 Msa0826580:CDS
CAGGTATTAGAAACGGTGAT+AGG 0.556544 5_3:-67082476 None:intergenic
AAGCCAAGACTCAGAAGGAT+GGG 0.568171 5_3:+67083792 Msa0826580:three_prime_UTR
TAATACCTGGGAACGTGTCA+AGG 0.569738 5_3:+67082490 Msa0826580:CDS
TGAATATTGGGCCATATGGT+TGG 0.572353 5_3:+67083886 Msa0826580:three_prime_UTR
ACGTTCCCAGGTATTAGAAA+CGG 0.576633 5_3:-67082483 None:intergenic
TAAGCCAAGACTCAGAAGGA+TGG 0.579557 5_3:+67083791 Msa0826580:three_prime_UTR
CGTCGTCGTTGTAGTTTGAG+TGG 0.581233 5_3:-67083693 None:intergenic
ATGAGATAAAACAATTGCCA+AGG 0.592832 5_3:-67084005 None:intergenic
TTTACACTAATGAATAACAT+AGG 0.594165 5_3:-67083949 None:intergenic
ACTGAAGCCGAAGCTGGGAG+CGG 0.599839 5_3:+67082379 Msa0826580:CDS
GCCATAAGCCAAGACTCAGA+AGG 0.628117 5_3:+67083787 Msa0826580:three_prime_UTR
GGTAACGCTGACAACGCCGC+CGG 0.632194 5_3:+67082400 Msa0826580:CDS
ACTGTTTATGAGCTCCAACA+TGG 0.634162 5_3:-67083637 None:intergenic
ATTGTTTGTCTCTGTTCCGG+CGG 0.660646 5_3:-67082416 None:intergenic
ACCTGGTTTGGTAAACCAAG+TGG 0.666171 5_3:-67083669 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTTCATAGTAATAATT+AGG - chr5_3:67082197-67082216 None:intergenic 15.0%
!!! TGCTATTTTAGAAATTAATT+TGG + chr5_3:67084077-67084096 Msa0826580:three_prime_UTR 15.0%
!! TTCATTTCATAGTAATAATT+AGG - chr5_3:67082197-67082216 None:intergenic 15.0%
!!! TGCTATTTTAGAAATTAATT+TGG + chr5_3:67084077-67084096 Msa0826580:three_prime_UTR 15.0%
!! TTAAAAACAAACATCATGTA+TGG - chr5_3:67082550-67082569 None:intergenic 20.0%
!! TTTACACTAATGAATAACAT+AGG - chr5_3:67083952-67083971 None:intergenic 20.0%
!! AAATAAGAAAAATCATCAAG+AGG + chr5_3:67084117-67084136 Msa0826580:three_prime_UTR 20.0%
!! AATAAGAAAAATCATCAAGA+GGG + chr5_3:67084118-67084137 Msa0826580:three_prime_UTR 20.0%
!! TTAAAAACAAACATCATGTA+TGG - chr5_3:67082550-67082569 None:intergenic 20.0%
!! TTTACACTAATGAATAACAT+AGG - chr5_3:67083952-67083971 None:intergenic 20.0%
!! AAATAAGAAAAATCATCAAG+AGG + chr5_3:67084117-67084136 Msa0826580:three_prime_UTR 20.0%
!! AATAAGAAAAATCATCAAGA+GGG + chr5_3:67084118-67084137 Msa0826580:three_prime_UTR 20.0%
! AATTAGAGTAGTGAGAAATT+AGG + chr5_3:67082307-67082326 Msa0826580:five_prime_UTR 25.0%
! ATTAGAGTAGTGAGAAATTA+GGG + chr5_3:67082308-67082327 Msa0826580:five_prime_UTR 25.0%
! TTTGATATTGTTAAGGAACA+TGG + chr5_3:67082452-67082471 Msa0826580:CDS 25.0%
!!! ATTGTTTCTGCATTATGTTT+TGG + chr5_3:67082584-67082603 Msa0826580:intron 25.0%
!!! TGTTTTGAAATAGTGATGTA+TGG + chr5_3:67082642-67082661 Msa0826580:intron 25.0%
! CATACTACATTAGATGTAAT+AGG + chr5_3:67082895-67082914 Msa0826580:intron 25.0%
! ACATAAGAATTTACATGGAT+CGG + chr5_3:67082920-67082939 Msa0826580:intron 25.0%
! TAAAACAGTGAAAAAATTGC+TGG - chr5_3:67082962-67082981 None:intergenic 25.0%
! AAAATTGTTACTCTGTTGAA+GGG + chr5_3:67083256-67083275 Msa0826580:intron 25.0%
!!! ATTGTTTTGTTTACTTGTGA+TGG + chr5_3:67083439-67083458 Msa0826580:intron 25.0%
! ACAGATATGTAACATTCATA+AGG - chr5_3:67083468-67083487 None:intergenic 25.0%
! CAATGAAGAATCTGAATATT+GGG + chr5_3:67083874-67083893 Msa0826580:three_prime_UTR 25.0%
! TGTTATTCATTAGTGTAAAC+TGG + chr5_3:67083953-67083972 Msa0826580:three_prime_UTR 25.0%
! TTATTTGTAGCTTCTCTAAA+TGG - chr5_3:67084103-67084122 None:intergenic 25.0%
! AATTAGAGTAGTGAGAAATT+AGG + chr5_3:67082307-67082326 Msa0826580:five_prime_UTR 25.0%
! ATTAGAGTAGTGAGAAATTA+GGG + chr5_3:67082308-67082327 Msa0826580:five_prime_UTR 25.0%
! TTTGATATTGTTAAGGAACA+TGG + chr5_3:67082452-67082471 Msa0826580:CDS 25.0%
!!! ATTGTTTCTGCATTATGTTT+TGG + chr5_3:67082584-67082603 Msa0826580:intron 25.0%
!!! TGTTTTGAAATAGTGATGTA+TGG + chr5_3:67082642-67082661 Msa0826580:intron 25.0%
! CATACTACATTAGATGTAAT+AGG + chr5_3:67082895-67082914 Msa0826580:intron 25.0%
! ACATAAGAATTTACATGGAT+CGG + chr5_3:67082920-67082939 Msa0826580:intron 25.0%
! TAAAACAGTGAAAAAATTGC+TGG - chr5_3:67082962-67082981 None:intergenic 25.0%
! AAAATTGTTACTCTGTTGAA+GGG + chr5_3:67083256-67083275 Msa0826580:intron 25.0%
!!! ATTGTTTTGTTTACTTGTGA+TGG + chr5_3:67083439-67083458 Msa0826580:intron 25.0%
! ACAGATATGTAACATTCATA+AGG - chr5_3:67083468-67083487 None:intergenic 25.0%
! CAATGAAGAATCTGAATATT+GGG + chr5_3:67083874-67083893 Msa0826580:three_prime_UTR 25.0%
! TGTTATTCATTAGTGTAAAC+TGG + chr5_3:67083953-67083972 Msa0826580:three_prime_UTR 25.0%
! TTATTTGTAGCTTCTCTAAA+TGG - chr5_3:67084103-67084122 None:intergenic 25.0%
AGCTCTCTTTGATATTGTTA+AGG + chr5_3:67082445-67082464 Msa0826580:CDS 30.0%
AGGACACATAAGAATTTACA+TGG + chr5_3:67082915-67082934 Msa0826580:intron 30.0%
TAGAATCTTGAAAACTTGTG+TGG + chr5_3:67083002-67083021 Msa0826580:intron 30.0%
GATTACTACTCAAGTTTACA+GGG - chr5_3:67083030-67083049 None:intergenic 30.0%
ATACAAACAAAATCACGTGA+AGG - chr5_3:67083155-67083174 None:intergenic 30.0%
!! ACGTGATTTTGTTTGTATAG+TGG + chr5_3:67083157-67083176 Msa0826580:intron 30.0%
!! TGATTTTGTTTGTATAGTGG+AGG + chr5_3:67083160-67083179 Msa0826580:intron 30.0%
GAAAATTGTTACTCTGTTGA+AGG + chr5_3:67083255-67083274 Msa0826580:intron 30.0%
TTACATATCTGTGCATCTAT+TGG + chr5_3:67083476-67083495 Msa0826580:intron 30.0%
AACTTCCTGGAAAAAATTGA+AGG - chr5_3:67083531-67083550 None:intergenic 30.0%
!!! TTCAATTTTTTCCAGGAAGT+TGG + chr5_3:67083530-67083549 Msa0826580:intron 30.0%
!!! ACATGAAAGTAGTTTTGAGA+TGG + chr5_3:67083579-67083598 Msa0826580:CDS 30.0%
! AAAGCATTCCTTCAAAGAAA+AGG + chr5_3:67083732-67083751 Msa0826580:CDS 30.0%
TTATGCAATGGGAAGAATAA+TGG + chr5_3:67083824-67083843 Msa0826580:three_prime_UTR 30.0%
CCAATATTCAGATTCTTCAT+TGG - chr5_3:67083876-67083895 None:intergenic 30.0%
CCAATGAAGAATCTGAATAT+TGG + chr5_3:67083873-67083892 Msa0826580:three_prime_UTR 30.0%
ATTTAACACAACCAACCATA+TGG - chr5_3:67083900-67083919 None:intergenic 30.0%
ATGAGATAAAACAATTGCCA+AGG - chr5_3:67084008-67084027 None:intergenic 30.0%
TCATTGTTAGCATTAGTTAG+TGG + chr5_3:67084024-67084043 Msa0826580:three_prime_UTR 30.0%
AGCTCTCTTTGATATTGTTA+AGG + chr5_3:67082445-67082464 Msa0826580:CDS 30.0%
AGGACACATAAGAATTTACA+TGG + chr5_3:67082915-67082934 Msa0826580:intron 30.0%
TAGAATCTTGAAAACTTGTG+TGG + chr5_3:67083002-67083021 Msa0826580:intron 30.0%
GATTACTACTCAAGTTTACA+GGG - chr5_3:67083030-67083049 None:intergenic 30.0%
ATACAAACAAAATCACGTGA+AGG - chr5_3:67083155-67083174 None:intergenic 30.0%
!! ACGTGATTTTGTTTGTATAG+TGG + chr5_3:67083157-67083176 Msa0826580:intron 30.0%
!! TGATTTTGTTTGTATAGTGG+AGG + chr5_3:67083160-67083179 Msa0826580:intron 30.0%
GAAAATTGTTACTCTGTTGA+AGG + chr5_3:67083255-67083274 Msa0826580:intron 30.0%
TTACATATCTGTGCATCTAT+TGG + chr5_3:67083476-67083495 Msa0826580:intron 30.0%
AACTTCCTGGAAAAAATTGA+AGG - chr5_3:67083531-67083550 None:intergenic 30.0%
!!! TTCAATTTTTTCCAGGAAGT+TGG + chr5_3:67083530-67083549 Msa0826580:intron 30.0%
!!! ACATGAAAGTAGTTTTGAGA+TGG + chr5_3:67083579-67083598 Msa0826580:CDS 30.0%
! AAAGCATTCCTTCAAAGAAA+AGG + chr5_3:67083732-67083751 Msa0826580:CDS 30.0%
TTATGCAATGGGAAGAATAA+TGG + chr5_3:67083824-67083843 Msa0826580:three_prime_UTR 30.0%
CCAATATTCAGATTCTTCAT+TGG - chr5_3:67083876-67083895 None:intergenic 30.0%
CCAATGAAGAATCTGAATAT+TGG + chr5_3:67083873-67083892 Msa0826580:three_prime_UTR 30.0%
ATTTAACACAACCAACCATA+TGG - chr5_3:67083900-67083919 None:intergenic 30.0%
ATGAGATAAAACAATTGCCA+AGG - chr5_3:67084008-67084027 None:intergenic 30.0%
TCATTGTTAGCATTAGTTAG+TGG + chr5_3:67084024-67084043 Msa0826580:three_prime_UTR 30.0%
CACAATTCAGAGCTTAACTT+AGG + chr5_3:67082146-67082165 Msa0826580:five_prime_UTR 35.0%
GGTTTGAGAAGAGAAATGTT+TGG + chr5_3:67082329-67082348 Msa0826580:exon 35.0%
GTTATTGTTTGTCTCTGTTC+CGG - chr5_3:67082422-67082441 None:intergenic 35.0%
TATCACCGTTTCTAATACCT+GGG + chr5_3:67082478-67082497 Msa0826580:CDS 35.0%
ATACATATCTACAGTGCTAG+TGG + chr5_3:67082805-67082824 Msa0826580:intron 35.0%
TACATATCTACAGTGCTAGT+GGG + chr5_3:67082806-67082825 Msa0826580:intron 35.0%
CATCTTCTCAGCATTAATTG+CGG - chr5_3:67082847-67082866 None:intergenic 35.0%
GCAATTAATGCTGAGAAGAT+GGG + chr5_3:67082846-67082865 Msa0826580:intron 35.0%
GGATTACTACTCAAGTTTAC+AGG - chr5_3:67083031-67083050 None:intergenic 35.0%
TAATCCACTCAAGGATTTCA+AGG + chr5_3:67083045-67083064 Msa0826580:intron 35.0%
TATTCCTTGAAATCCTTGAG+TGG - chr5_3:67083052-67083071 None:intergenic 35.0%
CCAGGATAAATGTGCAAATA+TGG - chr5_3:67083118-67083137 None:intergenic 35.0%
CCATATTTGCACATTTATCC+TGG + chr5_3:67083115-67083134 Msa0826580:intron 35.0%
AAGGATCAAAACTCAAATCC+AGG - chr5_3:67083136-67083155 None:intergenic 35.0%
!! TTTTGTTTGTATAGTGGAGG+TGG + chr5_3:67083163-67083182 Msa0826580:intron 35.0%
CTGGAAAAAATTGAAGGTCA+TGG - chr5_3:67083525-67083544 None:intergenic 35.0%
!!! CATGACCTTCAATTTTTTCC+AGG + chr5_3:67083523-67083542 Msa0826580:intron 35.0%
AACAGTTTCTCTATACCACT+TGG + chr5_3:67083654-67083673 Msa0826580:CDS 35.0%
AATCTGAATATTGGGCCATA+TGG + chr5_3:67083882-67083901 Msa0826580:three_prime_UTR 35.0%
AAATCATCAAGAGGGATTTC+AGG + chr5_3:67084126-67084145 Msa0826580:three_prime_UTR 35.0%
CACAATTCAGAGCTTAACTT+AGG + chr5_3:67082146-67082165 Msa0826580:five_prime_UTR 35.0%
GGTTTGAGAAGAGAAATGTT+TGG + chr5_3:67082329-67082348 Msa0826580:exon 35.0%
GTTATTGTTTGTCTCTGTTC+CGG - chr5_3:67082422-67082441 None:intergenic 35.0%
TATCACCGTTTCTAATACCT+GGG + chr5_3:67082478-67082497 Msa0826580:CDS 35.0%
ATACATATCTACAGTGCTAG+TGG + chr5_3:67082805-67082824 Msa0826580:intron 35.0%
TACATATCTACAGTGCTAGT+GGG + chr5_3:67082806-67082825 Msa0826580:intron 35.0%
CATCTTCTCAGCATTAATTG+CGG - chr5_3:67082847-67082866 None:intergenic 35.0%
GCAATTAATGCTGAGAAGAT+GGG + chr5_3:67082846-67082865 Msa0826580:intron 35.0%
GGATTACTACTCAAGTTTAC+AGG - chr5_3:67083031-67083050 None:intergenic 35.0%
TAATCCACTCAAGGATTTCA+AGG + chr5_3:67083045-67083064 Msa0826580:intron 35.0%
TATTCCTTGAAATCCTTGAG+TGG - chr5_3:67083052-67083071 None:intergenic 35.0%
CCAGGATAAATGTGCAAATA+TGG - chr5_3:67083118-67083137 None:intergenic 35.0%
CCATATTTGCACATTTATCC+TGG + chr5_3:67083115-67083134 Msa0826580:intron 35.0%
AAGGATCAAAACTCAAATCC+AGG - chr5_3:67083136-67083155 None:intergenic 35.0%
!! TTTTGTTTGTATAGTGGAGG+TGG + chr5_3:67083163-67083182 Msa0826580:intron 35.0%
CTGGAAAAAATTGAAGGTCA+TGG - chr5_3:67083525-67083544 None:intergenic 35.0%
!!! CATGACCTTCAATTTTTTCC+AGG + chr5_3:67083523-67083542 Msa0826580:intron 35.0%
AACAGTTTCTCTATACCACT+TGG + chr5_3:67083654-67083673 Msa0826580:CDS 35.0%
AATCTGAATATTGGGCCATA+TGG + chr5_3:67083882-67083901 Msa0826580:three_prime_UTR 35.0%
CAGGTATTAGAAACGGTGAT+AGG - chr5_3:67082479-67082498 None:intergenic 40.0%
CTATCACCGTTTCTAATACC+TGG + chr5_3:67082477-67082496 Msa0826580:CDS 40.0%
ACGTTCCCAGGTATTAGAAA+CGG - chr5_3:67082486-67082505 None:intergenic 40.0%
AATAATTCGCTATTGTGCCC+TGG + chr5_3:67082684-67082703 Msa0826580:intron 40.0%
CGCAATTAATGCTGAGAAGA+TGG + chr5_3:67082845-67082864 Msa0826580:intron 40.0%
TTTACATGGATCGGAGTCTA+AGG + chr5_3:67082929-67082948 Msa0826580:intron 40.0%
CTTGAGTAGTAATCCACTCA+AGG + chr5_3:67083036-67083055 Msa0826580:intron 40.0%
GATCTTTCAATCCAACTTCC+TGG - chr5_3:67083544-67083563 None:intergenic 40.0%
ACTGTTTATGAGCTCCAACA+TGG - chr5_3:67083640-67083659 None:intergenic 40.0%
TTCCCATTGCATAAAACAGC+TGG - chr5_3:67083818-67083837 None:intergenic 40.0%
TGAATATTGGGCCATATGGT+TGG + chr5_3:67083886-67083905 Msa0826580:three_prime_UTR 40.0%
! AGCACTATGTATTGATGCCT+TGG + chr5_3:67083988-67084007 Msa0826580:three_prime_UTR 40.0%
CAGGTATTAGAAACGGTGAT+AGG - chr5_3:67082479-67082498 None:intergenic 40.0%
CTATCACCGTTTCTAATACC+TGG + chr5_3:67082477-67082496 Msa0826580:CDS 40.0%
ACGTTCCCAGGTATTAGAAA+CGG - chr5_3:67082486-67082505 None:intergenic 40.0%
AATAATTCGCTATTGTGCCC+TGG + chr5_3:67082684-67082703 Msa0826580:intron 40.0%
CGCAATTAATGCTGAGAAGA+TGG + chr5_3:67082845-67082864 Msa0826580:intron 40.0%
TTTACATGGATCGGAGTCTA+AGG + chr5_3:67082929-67082948 Msa0826580:intron 40.0%
CTTGAGTAGTAATCCACTCA+AGG + chr5_3:67083036-67083055 Msa0826580:intron 40.0%
GATCTTTCAATCCAACTTCC+TGG - chr5_3:67083544-67083563 None:intergenic 40.0%
ACTGTTTATGAGCTCCAACA+TGG - chr5_3:67083640-67083659 None:intergenic 40.0%
TTCCCATTGCATAAAACAGC+TGG - chr5_3:67083818-67083837 None:intergenic 40.0%
TGAATATTGGGCCATATGGT+TGG + chr5_3:67083886-67083905 Msa0826580:three_prime_UTR 40.0%
! AGCACTATGTATTGATGCCT+TGG + chr5_3:67083988-67084007 Msa0826580:three_prime_UTR 40.0%
AAGAAACTGAAGCCGAAGCT+GGG + chr5_3:67082374-67082393 Msa0826580:CDS 45.0%
ATTGTTTGTCTCTGTTCCGG+CGG - chr5_3:67082419-67082438 None:intergenic 45.0%
TAATACCTGGGAACGTGTCA+AGG + chr5_3:67082490-67082509 Msa0826580:CDS 45.0%
AATTACCTTGACACGTTCCC+AGG - chr5_3:67082498-67082517 None:intergenic 45.0%
CTGTCCTAAATAGCGTACCA+GGG - chr5_3:67082704-67082723 None:intergenic 45.0%
ACTGTCCTAAATAGCGTACC+AGG - chr5_3:67082705-67082724 None:intergenic 45.0%
AGATGGGTGTGTTGGATGTT+TGG + chr5_3:67082862-67082881 Msa0826580:intron 45.0%
! TGTTTGTATAGTGGAGGTGG+AGG + chr5_3:67083166-67083185 Msa0826580:intron 45.0%
! GTTTGTATAGTGGAGGTGGA+GGG + chr5_3:67083167-67083186 Msa0826580:intron 45.0%
!! GAGGGAAAGTGACTTGTTCA+AGG + chr5_3:67083185-67083204 Msa0826580:intron 45.0%
!! CGTCTTGTTTGCAACCATGT+TGG + chr5_3:67083623-67083642 Msa0826580:CDS 45.0%
ACCACTTGGTTTACCAAACC+AGG + chr5_3:67083668-67083687 Msa0826580:CDS 45.0%
!! ACCTGGTTTGGTAAACCAAG+TGG - chr5_3:67083672-67083691 None:intergenic 45.0%
AGTTTGAGTGGTACCTGGTT+TGG - chr5_3:67083684-67083703 None:intergenic 45.0%
GTTGTAGTTTGAGTGGTACC+TGG - chr5_3:67083689-67083708 None:intergenic 45.0%
!!! CGTGCTAGCCTTTTCTTTGA+AGG - chr5_3:67083743-67083762 None:intergenic 45.0%
TCCTTCTGAGTCTTGGCTTA+TGG - chr5_3:67083791-67083810 None:intergenic 45.0%
TAAGCCAAGACTCAGAAGGA+TGG + chr5_3:67083791-67083810 Msa0826580:three_prime_UTR 45.0%
AAGCCAAGACTCAGAAGGAT+GGG + chr5_3:67083792-67083811 Msa0826580:three_prime_UTR 45.0%
!! GGCCAGCTGTTTTATGCAAT+GGG + chr5_3:67083813-67083832 Msa0826580:three_prime_UTR 45.0%
GGAACAGAAGCAACACAATC+AGG - chr5_3:67083931-67083950 None:intergenic 45.0%
AAGAAACTGAAGCCGAAGCT+GGG + chr5_3:67082374-67082393 Msa0826580:CDS 45.0%
ATTGTTTGTCTCTGTTCCGG+CGG - chr5_3:67082419-67082438 None:intergenic 45.0%
TAATACCTGGGAACGTGTCA+AGG + chr5_3:67082490-67082509 Msa0826580:CDS 45.0%
AATTACCTTGACACGTTCCC+AGG - chr5_3:67082498-67082517 None:intergenic 45.0%
CTGTCCTAAATAGCGTACCA+GGG - chr5_3:67082704-67082723 None:intergenic 45.0%
ACTGTCCTAAATAGCGTACC+AGG - chr5_3:67082705-67082724 None:intergenic 45.0%
AGATGGGTGTGTTGGATGTT+TGG + chr5_3:67082862-67082881 Msa0826580:intron 45.0%
! TGTTTGTATAGTGGAGGTGG+AGG + chr5_3:67083166-67083185 Msa0826580:intron 45.0%
! GTTTGTATAGTGGAGGTGGA+GGG + chr5_3:67083167-67083186 Msa0826580:intron 45.0%
!! GAGGGAAAGTGACTTGTTCA+AGG + chr5_3:67083185-67083204 Msa0826580:intron 45.0%
!! CGTCTTGTTTGCAACCATGT+TGG + chr5_3:67083623-67083642 Msa0826580:CDS 45.0%
ACCACTTGGTTTACCAAACC+AGG + chr5_3:67083668-67083687 Msa0826580:CDS 45.0%
!! ACCTGGTTTGGTAAACCAAG+TGG - chr5_3:67083672-67083691 None:intergenic 45.0%
AGTTTGAGTGGTACCTGGTT+TGG - chr5_3:67083684-67083703 None:intergenic 45.0%
GTTGTAGTTTGAGTGGTACC+TGG - chr5_3:67083689-67083708 None:intergenic 45.0%
!!! CGTGCTAGCCTTTTCTTTGA+AGG - chr5_3:67083743-67083762 None:intergenic 45.0%
TCCTTCTGAGTCTTGGCTTA+TGG - chr5_3:67083791-67083810 None:intergenic 45.0%
TAAGCCAAGACTCAGAAGGA+TGG + chr5_3:67083791-67083810 Msa0826580:three_prime_UTR 45.0%
AAGCCAAGACTCAGAAGGAT+GGG + chr5_3:67083792-67083811 Msa0826580:three_prime_UTR 45.0%
!! GGCCAGCTGTTTTATGCAAT+GGG + chr5_3:67083813-67083832 Msa0826580:three_prime_UTR 45.0%
GGAACAGAAGCAACACAATC+AGG - chr5_3:67083931-67083950 None:intergenic 45.0%
GAAGAAACTGAAGCCGAAGC+TGG + chr5_3:67082373-67082392 Msa0826580:CDS 50.0%
TGTGCCCTGGTACGCTATTT+AGG + chr5_3:67082697-67082716 Msa0826580:intron 50.0%
TGCTGAGAAGATGGGTGTGT+TGG + chr5_3:67082854-67082873 Msa0826580:intron 50.0%
CGTCGTCGTTGTAGTTTGAG+TGG - chr5_3:67083696-67083715 None:intergenic 50.0%
GCCATAAGCCAAGACTCAGA+AGG + chr5_3:67083787-67083806 Msa0826580:three_prime_UTR 50.0%
!! GGGCCAGCTGTTTTATGCAA+TGG + chr5_3:67083812-67083831 Msa0826580:three_prime_UTR 50.0%
GAAGAAACTGAAGCCGAAGC+TGG + chr5_3:67082373-67082392 Msa0826580:CDS 50.0%
TGTGCCCTGGTACGCTATTT+AGG + chr5_3:67082697-67082716 Msa0826580:intron 50.0%
TGCTGAGAAGATGGGTGTGT+TGG + chr5_3:67082854-67082873 Msa0826580:intron 50.0%
CGTCGTCGTTGTAGTTTGAG+TGG - chr5_3:67083696-67083715 None:intergenic 50.0%
GCCATAAGCCAAGACTCAGA+AGG + chr5_3:67083787-67083806 Msa0826580:three_prime_UTR 50.0%
!! GGGCCAGCTGTTTTATGCAA+TGG + chr5_3:67083812-67083831 Msa0826580:three_prime_UTR 50.0%
TGGCCCATCCTTCTGAGTCT+TGG - chr5_3:67083798-67083817 None:intergenic 55.0%
TGGCCCATCCTTCTGAGTCT+TGG - chr5_3:67083798-67083817 None:intergenic 55.0%
ACTGAAGCCGAAGCTGGGAG+CGG + chr5_3:67082379-67082398 Msa0826580:CDS 60.0%
AGCGTTACCGCTCCCAGCTT+CGG - chr5_3:67082389-67082408 None:intergenic 60.0%
ACTGAAGCCGAAGCTGGGAG+CGG + chr5_3:67082379-67082398 Msa0826580:CDS 60.0%
AGCGTTACCGCTCCCAGCTT+CGG - chr5_3:67082389-67082408 None:intergenic 60.0%
GGTAACGCTGACAACGCCGC+CGG + chr5_3:67082400-67082419 Msa0826580:CDS 65.0%
GGTAACGCTGACAACGCCGC+CGG + chr5_3:67082400-67082419 Msa0826580:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 67082113 67084147 67082113 ID=Msa0826580;Name=Msa0826580
chr5_3 mRNA 67082113 67084147 67082113 ID=Msa0826580-mRNA-1;Parent=Msa0826580;Name=Msa0826580-mRNA-1;_AED=0.07;_eAED=0.07;_QI=112|1|1|1|0.5|0.33|3|378|130
chr5_3 exon 67082113 67082167 67082113 ID=Msa0826580-mRNA-1:exon:21043;Parent=Msa0826580-mRNA-1
chr5_3 exon 67082287 67082511 67082287 ID=Msa0826580-mRNA-1:exon:21044;Parent=Msa0826580-mRNA-1
chr5_3 exon 67083545 67084147 67083545 ID=Msa0826580-mRNA-1:exon:21045;Parent=Msa0826580-mRNA-1
chr5_3 five_prime_UTR 67082113 67082167 67082113 ID=Msa0826580-mRNA-1:five_prime_utr;Parent=Msa0826580-mRNA-1
chr5_3 five_prime_UTR 67082287 67082343 67082287 ID=Msa0826580-mRNA-1:five_prime_utr;Parent=Msa0826580-mRNA-1
chr5_3 CDS 67082344 67082511 67082344 ID=Msa0826580-mRNA-1:cds;Parent=Msa0826580-mRNA-1
chr5_3 CDS 67083545 67083769 67083545 ID=Msa0826580-mRNA-1:cds;Parent=Msa0826580-mRNA-1
chr5_3 three_prime_UTR 67083770 67084147 67083770 ID=Msa0826580-mRNA-1:three_prime_utr;Parent=Msa0826580-mRNA-1
Gene Sequence

>Msa0826580

ATGTTTGGAACAGCGATTAGAAATGCAGCGAAGAAACTGAAGCCGAAGCTGGGAGCGGTAACGCTGACAACGCCGCCGGAACAGAGACAAACAATAACAAGAGCTCTCTTTGATATTGTTAAGGAACATGGTCCTATCACCGTTTCTAATACCTGGGAACGTGTCAAGGAAGTTGGATTGAAAGATCTGACAAGCAAGAATCACATGAAAGTAGTTTTGAGATGGATGAGAGAGAGACAGAAGCTTCGTCTTGTTTGCAACCATGTTGGAGCTCATAAACAGTTTCTCTATACCACTTGGTTTACCAAACCAGGTACCACTCAAACTACAACGACGACGACAACAACAACAACGAAAAGCATTCCTTCAAAGAAAAGGCTAGCACGATCTTGA
Protein sequence

>Msa0826580

MFGTAIRNAAKKLKPKLGAVTLTTPPEQRQTITRALFDIVKEHGPITVSNTWERVKEVGLKDLTSKNHMKVVLRWMRERQKLRLVCNHVGAHKQFLYTTWFTKPGTTQTTTTTTTTTTKSIPSKKRLARS*