AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0864630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0864630 MtrunA17_Chr5g0447491 100.000 130 0 0 1 130 1 130 1.18e-92 263
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0864630 AT5G49410.1 69.307 101 31 0 3 103 18 118 2.55e-51 159
Msa0864630 AT5G49410.2 69.307 101 31 0 3 103 18 118 2.55e-51 159
Msa0864630 AT1G73940.1 37.069 116 64 2 11 126 43 149 3.73e-23 88.6

Find 48 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTGTAGCTTCTCTAAA+TGG 0.231985 5_4:-65120161 None:intergenic
TCCTTCTGAGTCTTGGCTTA+TGG 0.232824 5_4:-65119849 None:intergenic
AATTAGAGTAGTGAGAAATT+AGG 0.247656 5_4:+65118368 Msa0864630:five_prime_UTR
ATTAGAGTAGTGAGAAATTA+GGG 0.258869 5_4:+65118369 Msa0864630:five_prime_UTR
TGTTATTCATTAGTGTAAAC+TGG 0.347893 5_4:+65120014 Msa0864630:three_prime_UTR
GTTATTGTTTGTCTCTGTTC+CGG 0.354082 5_4:-65118480 None:intergenic
TTATGCAATGGGAAGAATAA+TGG 0.354896 5_4:+65119885 Msa0864630:three_prime_UTR
CCAATGAAGAATCTGAATAC+TGG 0.366030 5_4:+65119934 Msa0864630:three_prime_UTR
TGGCCCATCCTTCTGAGTCT+TGG 0.377407 5_4:-65119856 None:intergenic
AGCTCTCTTTGATATTGTTA+AGG 0.392484 5_4:+65118506 Msa0864630:CDS
GGAACAGAAGCAACACAATC+AGG 0.417769 5_4:-65119989 None:intergenic
CCAGTATTCAGATTCTTCAT+TGG 0.421543 5_4:-65119934 None:intergenic
CTATCACCGTTTCTAATACC+TGG 0.429853 5_4:+65118538 Msa0864630:CDS
TCATTGTTAGCATTAGTTAG+TGG 0.441886 5_4:+65120085 Msa0864630:three_prime_UTR
AGCGTTACCGCTCCCAGCTT+CGG 0.442104 5_4:-65118447 None:intergenic
AGTTTGAGTGGTACCTGGTT+TGG 0.446620 5_4:-65119742 None:intergenic
GAAGAAACTGAAGCCGAAGC+TGG 0.455351 5_4:+65118434 Msa0864630:CDS
ATTTAACACAACCAACCATA+TGG 0.462311 5_4:-65119958 None:intergenic
AATTACCTTGACACGTTCCC+AGG 0.464678 5_4:-65118556 None:intergenic
AAAGCATTCCTTCAAAGAAA+AGG 0.466561 5_4:+65119793 Msa0864630:CDS
AGCACTATGTATTGATGCCT+TGG 0.468698 5_4:+65120049 Msa0864630:three_prime_UTR
CGTCTTGTTTGCAACCATGT+TGG 0.476670 5_4:+65119684 Msa0864630:CDS
TTCCCATTGCATAAAACAGC+TGG 0.482960 5_4:-65119876 None:intergenic
AATCTGAATACTGGGCCATA+TGG 0.489508 5_4:+65119943 Msa0864630:three_prime_UTR
GTTGTAGTTTGAGTGGTACC+TGG 0.498914 5_4:-65119747 None:intergenic
CAATGAAGAATCTGAATACT+GGG 0.500380 5_4:+65119935 Msa0864630:three_prime_UTR
GGTTTGAGAAGAGAAATGTT+TGG 0.500931 5_4:+65118390 Msa0864630:exon
CACAATTCAGAGCTTAACTT+AGG 0.505427 5_4:+65118207 Msa0864630:five_prime_UTR
ACCACTTGGTTTACCAAACC+AGG 0.536017 5_4:+65119729 Msa0864630:CDS
TTTGATATTGTTAAGGAACA+TGG 0.539683 5_4:+65118513 Msa0864630:CDS
TATCACCGTTTCTAATACCT+GGG 0.548790 5_4:+65118539 Msa0864630:CDS
AACAGTTTCTCTATACCACT+TGG 0.548939 5_4:+65119715 Msa0864630:CDS
AAGAAACTGAAGCCGAAGCT+GGG 0.553771 5_4:+65118435 Msa0864630:CDS
CAGGTATTAGAAACGGTGAT+AGG 0.556544 5_4:-65118537 None:intergenic
AAGCCAAGACTCAGAAGGAT+GGG 0.568171 5_4:+65119853 Msa0864630:three_prime_UTR
TAATACCTGGGAACGTGTCA+AGG 0.569738 5_4:+65118551 Msa0864630:CDS
ACGTTCCCAGGTATTAGAAA+CGG 0.576633 5_4:-65118544 None:intergenic
TAAGCCAAGACTCAGAAGGA+TGG 0.579557 5_4:+65119852 Msa0864630:three_prime_UTR
CGTCGTCGTTGTAGTTTGAG+TGG 0.581233 5_4:-65119754 None:intergenic
ATGAGATAAAACAATTGCCA+AGG 0.592832 5_4:-65120066 None:intergenic
TTTACACTAATGAATAACAT+AGG 0.594165 5_4:-65120010 None:intergenic
TGAATACTGGGCCATATGGT+TGG 0.598685 5_4:+65119947 Msa0864630:three_prime_UTR
ACTGAAGCCGAAGCTGGGAG+CGG 0.599839 5_4:+65118440 Msa0864630:CDS
GCCATAAGCCAAGACTCAGA+AGG 0.628117 5_4:+65119848 Msa0864630:three_prime_UTR
GGTAACGCTGACAACGCCGC+CGG 0.632194 5_4:+65118461 Msa0864630:CDS
ACTGTTTATGAGCTCCAACA+TGG 0.634162 5_4:-65119698 None:intergenic
ATTGTTTGTCTCTGTTCCGG+CGG 0.660646 5_4:-65118477 None:intergenic
ACCTGGTTTGGTAAACCAAG+TGG 0.666171 5_4:-65119730 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTTCATAGTAATAATT+AGG - chr5_4:65118258-65118277 None:intergenic 15.0%
!!! TGCTATTTTAGAAATTAATT+TGG + chr5_4:65120138-65120157 Msa0864630:three_prime_UTR 15.0%
!! TTCATTTCATAGTAATAATT+AGG - chr5_4:65118258-65118277 None:intergenic 15.0%
!!! TGCTATTTTAGAAATTAATT+TGG + chr5_4:65120138-65120157 Msa0864630:three_prime_UTR 15.0%
!! TTAAAAACAAACATCATGTA+TGG - chr5_4:65118611-65118630 None:intergenic 20.0%
!! TTTACACTAATGAATAACAT+AGG - chr5_4:65120013-65120032 None:intergenic 20.0%
!! AAATAAGAAAAATCATCAAG+AGG + chr5_4:65120178-65120197 Msa0864630:three_prime_UTR 20.0%
!! AATAAGAAAAATCATCAAGA+GGG + chr5_4:65120179-65120198 Msa0864630:three_prime_UTR 20.0%
!! TTAAAAACAAACATCATGTA+TGG - chr5_4:65118611-65118630 None:intergenic 20.0%
!! TTTACACTAATGAATAACAT+AGG - chr5_4:65120013-65120032 None:intergenic 20.0%
!! AAATAAGAAAAATCATCAAG+AGG + chr5_4:65120178-65120197 Msa0864630:three_prime_UTR 20.0%
!! AATAAGAAAAATCATCAAGA+GGG + chr5_4:65120179-65120198 Msa0864630:three_prime_UTR 20.0%
! AATTAGAGTAGTGAGAAATT+AGG + chr5_4:65118368-65118387 Msa0864630:five_prime_UTR 25.0%
! ATTAGAGTAGTGAGAAATTA+GGG + chr5_4:65118369-65118388 Msa0864630:five_prime_UTR 25.0%
! TTTGATATTGTTAAGGAACA+TGG + chr5_4:65118513-65118532 Msa0864630:CDS 25.0%
!!! ATTGTTTCTGCATTATGTTT+TGG + chr5_4:65118645-65118664 Msa0864630:intron 25.0%
!!! TGTTTTGAAATAGTGATGTA+TGG + chr5_4:65118703-65118722 Msa0864630:intron 25.0%
! CATACTACATTAGATGTAAT+AGG + chr5_4:65118956-65118975 Msa0864630:intron 25.0%
! ACATAAGAATTTACATGGAT+CGG + chr5_4:65118981-65119000 Msa0864630:intron 25.0%
! TAAAACAGTGAAAAAATTGC+TGG - chr5_4:65119023-65119042 None:intergenic 25.0%
! AAAATTGTTACTCTGTTGAA+GGG + chr5_4:65119317-65119336 Msa0864630:intron 25.0%
!!! ATTGTTTTGTTTACTTGTGA+TGG + chr5_4:65119500-65119519 Msa0864630:intron 25.0%
! ACAGATATGTAACATTCATA+AGG - chr5_4:65119529-65119548 None:intergenic 25.0%
! TGTTATTCATTAGTGTAAAC+TGG + chr5_4:65120014-65120033 Msa0864630:three_prime_UTR 25.0%
! TTATTTGTAGCTTCTCTAAA+TGG - chr5_4:65120164-65120183 None:intergenic 25.0%
! AATTAGAGTAGTGAGAAATT+AGG + chr5_4:65118368-65118387 Msa0864630:five_prime_UTR 25.0%
! ATTAGAGTAGTGAGAAATTA+GGG + chr5_4:65118369-65118388 Msa0864630:five_prime_UTR 25.0%
! TTTGATATTGTTAAGGAACA+TGG + chr5_4:65118513-65118532 Msa0864630:CDS 25.0%
!!! ATTGTTTCTGCATTATGTTT+TGG + chr5_4:65118645-65118664 Msa0864630:intron 25.0%
!!! TGTTTTGAAATAGTGATGTA+TGG + chr5_4:65118703-65118722 Msa0864630:intron 25.0%
! CATACTACATTAGATGTAAT+AGG + chr5_4:65118956-65118975 Msa0864630:intron 25.0%
! ACATAAGAATTTACATGGAT+CGG + chr5_4:65118981-65119000 Msa0864630:intron 25.0%
! TAAAACAGTGAAAAAATTGC+TGG - chr5_4:65119023-65119042 None:intergenic 25.0%
! AAAATTGTTACTCTGTTGAA+GGG + chr5_4:65119317-65119336 Msa0864630:intron 25.0%
!!! ATTGTTTTGTTTACTTGTGA+TGG + chr5_4:65119500-65119519 Msa0864630:intron 25.0%
! ACAGATATGTAACATTCATA+AGG - chr5_4:65119529-65119548 None:intergenic 25.0%
! TGTTATTCATTAGTGTAAAC+TGG + chr5_4:65120014-65120033 Msa0864630:three_prime_UTR 25.0%
! TTATTTGTAGCTTCTCTAAA+TGG - chr5_4:65120164-65120183 None:intergenic 25.0%
AGCTCTCTTTGATATTGTTA+AGG + chr5_4:65118506-65118525 Msa0864630:CDS 30.0%
AGGACACATAAGAATTTACA+TGG + chr5_4:65118976-65118995 Msa0864630:intron 30.0%
TAGAATCTTGAAAACTTGTG+TGG + chr5_4:65119063-65119082 Msa0864630:intron 30.0%
GATTACTACTCAAGTTTACA+GGG - chr5_4:65119091-65119110 None:intergenic 30.0%
ATACAAACAAAATCACGTGA+AGG - chr5_4:65119216-65119235 None:intergenic 30.0%
!! ACGTGATTTTGTTTGTATAG+TGG + chr5_4:65119218-65119237 Msa0864630:intron 30.0%
!! TGATTTTGTTTGTATAGTGG+AGG + chr5_4:65119221-65119240 Msa0864630:intron 30.0%
GAAAATTGTTACTCTGTTGA+AGG + chr5_4:65119316-65119335 Msa0864630:intron 30.0%
TTACATATCTGTGCATCTAT+TGG + chr5_4:65119537-65119556 Msa0864630:intron 30.0%
AACTTCCTGGAAAAAATTGA+AGG - chr5_4:65119592-65119611 None:intergenic 30.0%
!!! TTCAATTTTTTCCAGGAAGT+TGG + chr5_4:65119591-65119610 Msa0864630:intron 30.0%
!!! ACATGAAAGTAGTTTTGAGA+TGG + chr5_4:65119640-65119659 Msa0864630:CDS 30.0%
! AAAGCATTCCTTCAAAGAAA+AGG + chr5_4:65119793-65119812 Msa0864630:CDS 30.0%
TTATGCAATGGGAAGAATAA+TGG + chr5_4:65119885-65119904 Msa0864630:three_prime_UTR 30.0%
CAATGAAGAATCTGAATACT+GGG + chr5_4:65119935-65119954 Msa0864630:three_prime_UTR 30.0%
ATTTAACACAACCAACCATA+TGG - chr5_4:65119961-65119980 None:intergenic 30.0%
ATGAGATAAAACAATTGCCA+AGG - chr5_4:65120069-65120088 None:intergenic 30.0%
TCATTGTTAGCATTAGTTAG+TGG + chr5_4:65120085-65120104 Msa0864630:three_prime_UTR 30.0%
AGCTCTCTTTGATATTGTTA+AGG + chr5_4:65118506-65118525 Msa0864630:CDS 30.0%
AGGACACATAAGAATTTACA+TGG + chr5_4:65118976-65118995 Msa0864630:intron 30.0%
TAGAATCTTGAAAACTTGTG+TGG + chr5_4:65119063-65119082 Msa0864630:intron 30.0%
GATTACTACTCAAGTTTACA+GGG - chr5_4:65119091-65119110 None:intergenic 30.0%
ATACAAACAAAATCACGTGA+AGG - chr5_4:65119216-65119235 None:intergenic 30.0%
!! ACGTGATTTTGTTTGTATAG+TGG + chr5_4:65119218-65119237 Msa0864630:intron 30.0%
!! TGATTTTGTTTGTATAGTGG+AGG + chr5_4:65119221-65119240 Msa0864630:intron 30.0%
GAAAATTGTTACTCTGTTGA+AGG + chr5_4:65119316-65119335 Msa0864630:intron 30.0%
TTACATATCTGTGCATCTAT+TGG + chr5_4:65119537-65119556 Msa0864630:intron 30.0%
AACTTCCTGGAAAAAATTGA+AGG - chr5_4:65119592-65119611 None:intergenic 30.0%
!!! TTCAATTTTTTCCAGGAAGT+TGG + chr5_4:65119591-65119610 Msa0864630:intron 30.0%
!!! ACATGAAAGTAGTTTTGAGA+TGG + chr5_4:65119640-65119659 Msa0864630:CDS 30.0%
! AAAGCATTCCTTCAAAGAAA+AGG + chr5_4:65119793-65119812 Msa0864630:CDS 30.0%
TTATGCAATGGGAAGAATAA+TGG + chr5_4:65119885-65119904 Msa0864630:three_prime_UTR 30.0%
CAATGAAGAATCTGAATACT+GGG + chr5_4:65119935-65119954 Msa0864630:three_prime_UTR 30.0%
ATTTAACACAACCAACCATA+TGG - chr5_4:65119961-65119980 None:intergenic 30.0%
ATGAGATAAAACAATTGCCA+AGG - chr5_4:65120069-65120088 None:intergenic 30.0%
TCATTGTTAGCATTAGTTAG+TGG + chr5_4:65120085-65120104 Msa0864630:three_prime_UTR 30.0%
CACAATTCAGAGCTTAACTT+AGG + chr5_4:65118207-65118226 Msa0864630:five_prime_UTR 35.0%
GGTTTGAGAAGAGAAATGTT+TGG + chr5_4:65118390-65118409 Msa0864630:exon 35.0%
GTTATTGTTTGTCTCTGTTC+CGG - chr5_4:65118483-65118502 None:intergenic 35.0%
TATCACCGTTTCTAATACCT+GGG + chr5_4:65118539-65118558 Msa0864630:CDS 35.0%
ATACATATCTACAGTGCTAG+TGG + chr5_4:65118866-65118885 Msa0864630:intron 35.0%
TACATATCTACAGTGCTAGT+GGG + chr5_4:65118867-65118886 Msa0864630:intron 35.0%
CATCTTCTCAGCATTAATTG+CGG - chr5_4:65118908-65118927 None:intergenic 35.0%
GCAATTAATGCTGAGAAGAT+GGG + chr5_4:65118907-65118926 Msa0864630:intron 35.0%
GGATTACTACTCAAGTTTAC+AGG - chr5_4:65119092-65119111 None:intergenic 35.0%
TAATCCACTCAAGGATTTCA+AGG + chr5_4:65119106-65119125 Msa0864630:intron 35.0%
TATTCCTTGAAATCCTTGAG+TGG - chr5_4:65119113-65119132 None:intergenic 35.0%
CCAGGATAAATGTGCAAATA+TGG - chr5_4:65119179-65119198 None:intergenic 35.0%
CCATATTTGCACATTTATCC+TGG + chr5_4:65119176-65119195 Msa0864630:intron 35.0%
AAGGATCAAAACTCAAATCC+AGG - chr5_4:65119197-65119216 None:intergenic 35.0%
!! TTTTGTTTGTATAGTGGAGG+TGG + chr5_4:65119224-65119243 Msa0864630:intron 35.0%
CTGGAAAAAATTGAAGGTCA+TGG - chr5_4:65119586-65119605 None:intergenic 35.0%
!!! CATGACCTTCAATTTTTTCC+AGG + chr5_4:65119584-65119603 Msa0864630:intron 35.0%
AACAGTTTCTCTATACCACT+TGG + chr5_4:65119715-65119734 Msa0864630:CDS 35.0%
CCAGTATTCAGATTCTTCAT+TGG - chr5_4:65119937-65119956 None:intergenic 35.0%
CCAATGAAGAATCTGAATAC+TGG + chr5_4:65119934-65119953 Msa0864630:three_prime_UTR 35.0%
AAATCATCAAGAGGGATTTC+AGG + chr5_4:65120187-65120206 Msa0864630:three_prime_UTR 35.0%
CACAATTCAGAGCTTAACTT+AGG + chr5_4:65118207-65118226 Msa0864630:five_prime_UTR 35.0%
GGTTTGAGAAGAGAAATGTT+TGG + chr5_4:65118390-65118409 Msa0864630:exon 35.0%
GTTATTGTTTGTCTCTGTTC+CGG - chr5_4:65118483-65118502 None:intergenic 35.0%
TATCACCGTTTCTAATACCT+GGG + chr5_4:65118539-65118558 Msa0864630:CDS 35.0%
ATACATATCTACAGTGCTAG+TGG + chr5_4:65118866-65118885 Msa0864630:intron 35.0%
TACATATCTACAGTGCTAGT+GGG + chr5_4:65118867-65118886 Msa0864630:intron 35.0%
CATCTTCTCAGCATTAATTG+CGG - chr5_4:65118908-65118927 None:intergenic 35.0%
GCAATTAATGCTGAGAAGAT+GGG + chr5_4:65118907-65118926 Msa0864630:intron 35.0%
GGATTACTACTCAAGTTTAC+AGG - chr5_4:65119092-65119111 None:intergenic 35.0%
TAATCCACTCAAGGATTTCA+AGG + chr5_4:65119106-65119125 Msa0864630:intron 35.0%
TATTCCTTGAAATCCTTGAG+TGG - chr5_4:65119113-65119132 None:intergenic 35.0%
CCAGGATAAATGTGCAAATA+TGG - chr5_4:65119179-65119198 None:intergenic 35.0%
CCATATTTGCACATTTATCC+TGG + chr5_4:65119176-65119195 Msa0864630:intron 35.0%
AAGGATCAAAACTCAAATCC+AGG - chr5_4:65119197-65119216 None:intergenic 35.0%
!! TTTTGTTTGTATAGTGGAGG+TGG + chr5_4:65119224-65119243 Msa0864630:intron 35.0%
CTGGAAAAAATTGAAGGTCA+TGG - chr5_4:65119586-65119605 None:intergenic 35.0%
!!! CATGACCTTCAATTTTTTCC+AGG + chr5_4:65119584-65119603 Msa0864630:intron 35.0%
AACAGTTTCTCTATACCACT+TGG + chr5_4:65119715-65119734 Msa0864630:CDS 35.0%
CCAGTATTCAGATTCTTCAT+TGG - chr5_4:65119937-65119956 None:intergenic 35.0%
CCAATGAAGAATCTGAATAC+TGG + chr5_4:65119934-65119953 Msa0864630:three_prime_UTR 35.0%
CAGGTATTAGAAACGGTGAT+AGG - chr5_4:65118540-65118559 None:intergenic 40.0%
CTATCACCGTTTCTAATACC+TGG + chr5_4:65118538-65118557 Msa0864630:CDS 40.0%
ACGTTCCCAGGTATTAGAAA+CGG - chr5_4:65118547-65118566 None:intergenic 40.0%
AATAATTCGCTATTGTGCCC+TGG + chr5_4:65118745-65118764 Msa0864630:intron 40.0%
CGCAATTAATGCTGAGAAGA+TGG + chr5_4:65118906-65118925 Msa0864630:intron 40.0%
TTTACATGGATCGGAGTCTA+AGG + chr5_4:65118990-65119009 Msa0864630:intron 40.0%
CTTGAGTAGTAATCCACTCA+AGG + chr5_4:65119097-65119116 Msa0864630:intron 40.0%
GATCTTTCAATCCAACTTCC+TGG - chr5_4:65119605-65119624 None:intergenic 40.0%
ACTGTTTATGAGCTCCAACA+TGG - chr5_4:65119701-65119720 None:intergenic 40.0%
TTCCCATTGCATAAAACAGC+TGG - chr5_4:65119879-65119898 None:intergenic 40.0%
AATCTGAATACTGGGCCATA+TGG + chr5_4:65119943-65119962 Msa0864630:three_prime_UTR 40.0%
! AGCACTATGTATTGATGCCT+TGG + chr5_4:65120049-65120068 Msa0864630:three_prime_UTR 40.0%
CAGGTATTAGAAACGGTGAT+AGG - chr5_4:65118540-65118559 None:intergenic 40.0%
CTATCACCGTTTCTAATACC+TGG + chr5_4:65118538-65118557 Msa0864630:CDS 40.0%
ACGTTCCCAGGTATTAGAAA+CGG - chr5_4:65118547-65118566 None:intergenic 40.0%
AATAATTCGCTATTGTGCCC+TGG + chr5_4:65118745-65118764 Msa0864630:intron 40.0%
CGCAATTAATGCTGAGAAGA+TGG + chr5_4:65118906-65118925 Msa0864630:intron 40.0%
TTTACATGGATCGGAGTCTA+AGG + chr5_4:65118990-65119009 Msa0864630:intron 40.0%
CTTGAGTAGTAATCCACTCA+AGG + chr5_4:65119097-65119116 Msa0864630:intron 40.0%
GATCTTTCAATCCAACTTCC+TGG - chr5_4:65119605-65119624 None:intergenic 40.0%
ACTGTTTATGAGCTCCAACA+TGG - chr5_4:65119701-65119720 None:intergenic 40.0%
TTCCCATTGCATAAAACAGC+TGG - chr5_4:65119879-65119898 None:intergenic 40.0%
AATCTGAATACTGGGCCATA+TGG + chr5_4:65119943-65119962 Msa0864630:three_prime_UTR 40.0%
! AGCACTATGTATTGATGCCT+TGG + chr5_4:65120049-65120068 Msa0864630:three_prime_UTR 40.0%
AAGAAACTGAAGCCGAAGCT+GGG + chr5_4:65118435-65118454 Msa0864630:CDS 45.0%
ATTGTTTGTCTCTGTTCCGG+CGG - chr5_4:65118480-65118499 None:intergenic 45.0%
TAATACCTGGGAACGTGTCA+AGG + chr5_4:65118551-65118570 Msa0864630:CDS 45.0%
AATTACCTTGACACGTTCCC+AGG - chr5_4:65118559-65118578 None:intergenic 45.0%
CTGTCCTAAATAGCGTACCA+GGG - chr5_4:65118765-65118784 None:intergenic 45.0%
ACTGTCCTAAATAGCGTACC+AGG - chr5_4:65118766-65118785 None:intergenic 45.0%
AGATGGGTGTGTTGGATGTT+TGG + chr5_4:65118923-65118942 Msa0864630:intron 45.0%
! TGTTTGTATAGTGGAGGTGG+AGG + chr5_4:65119227-65119246 Msa0864630:intron 45.0%
! GTTTGTATAGTGGAGGTGGA+GGG + chr5_4:65119228-65119247 Msa0864630:intron 45.0%
!! GAGGGAAAGTGACTTGTTCA+AGG + chr5_4:65119246-65119265 Msa0864630:intron 45.0%
!! CGTCTTGTTTGCAACCATGT+TGG + chr5_4:65119684-65119703 Msa0864630:CDS 45.0%
ACCACTTGGTTTACCAAACC+AGG + chr5_4:65119729-65119748 Msa0864630:CDS 45.0%
!! ACCTGGTTTGGTAAACCAAG+TGG - chr5_4:65119733-65119752 None:intergenic 45.0%
AGTTTGAGTGGTACCTGGTT+TGG - chr5_4:65119745-65119764 None:intergenic 45.0%
GTTGTAGTTTGAGTGGTACC+TGG - chr5_4:65119750-65119769 None:intergenic 45.0%
!!! CGTGCTAGCCTTTTCTTTGA+AGG - chr5_4:65119804-65119823 None:intergenic 45.0%
TCCTTCTGAGTCTTGGCTTA+TGG - chr5_4:65119852-65119871 None:intergenic 45.0%
TAAGCCAAGACTCAGAAGGA+TGG + chr5_4:65119852-65119871 Msa0864630:three_prime_UTR 45.0%
AAGCCAAGACTCAGAAGGAT+GGG + chr5_4:65119853-65119872 Msa0864630:three_prime_UTR 45.0%
!! GGCCAGCTGTTTTATGCAAT+GGG + chr5_4:65119874-65119893 Msa0864630:three_prime_UTR 45.0%
TGAATACTGGGCCATATGGT+TGG + chr5_4:65119947-65119966 Msa0864630:three_prime_UTR 45.0%
GGAACAGAAGCAACACAATC+AGG - chr5_4:65119992-65120011 None:intergenic 45.0%
AAGAAACTGAAGCCGAAGCT+GGG + chr5_4:65118435-65118454 Msa0864630:CDS 45.0%
ATTGTTTGTCTCTGTTCCGG+CGG - chr5_4:65118480-65118499 None:intergenic 45.0%
TAATACCTGGGAACGTGTCA+AGG + chr5_4:65118551-65118570 Msa0864630:CDS 45.0%
AATTACCTTGACACGTTCCC+AGG - chr5_4:65118559-65118578 None:intergenic 45.0%
CTGTCCTAAATAGCGTACCA+GGG - chr5_4:65118765-65118784 None:intergenic 45.0%
ACTGTCCTAAATAGCGTACC+AGG - chr5_4:65118766-65118785 None:intergenic 45.0%
AGATGGGTGTGTTGGATGTT+TGG + chr5_4:65118923-65118942 Msa0864630:intron 45.0%
! TGTTTGTATAGTGGAGGTGG+AGG + chr5_4:65119227-65119246 Msa0864630:intron 45.0%
! GTTTGTATAGTGGAGGTGGA+GGG + chr5_4:65119228-65119247 Msa0864630:intron 45.0%
!! GAGGGAAAGTGACTTGTTCA+AGG + chr5_4:65119246-65119265 Msa0864630:intron 45.0%
!! CGTCTTGTTTGCAACCATGT+TGG + chr5_4:65119684-65119703 Msa0864630:CDS 45.0%
ACCACTTGGTTTACCAAACC+AGG + chr5_4:65119729-65119748 Msa0864630:CDS 45.0%
!! ACCTGGTTTGGTAAACCAAG+TGG - chr5_4:65119733-65119752 None:intergenic 45.0%
AGTTTGAGTGGTACCTGGTT+TGG - chr5_4:65119745-65119764 None:intergenic 45.0%
GTTGTAGTTTGAGTGGTACC+TGG - chr5_4:65119750-65119769 None:intergenic 45.0%
!!! CGTGCTAGCCTTTTCTTTGA+AGG - chr5_4:65119804-65119823 None:intergenic 45.0%
TCCTTCTGAGTCTTGGCTTA+TGG - chr5_4:65119852-65119871 None:intergenic 45.0%
TAAGCCAAGACTCAGAAGGA+TGG + chr5_4:65119852-65119871 Msa0864630:three_prime_UTR 45.0%
AAGCCAAGACTCAGAAGGAT+GGG + chr5_4:65119853-65119872 Msa0864630:three_prime_UTR 45.0%
!! GGCCAGCTGTTTTATGCAAT+GGG + chr5_4:65119874-65119893 Msa0864630:three_prime_UTR 45.0%
TGAATACTGGGCCATATGGT+TGG + chr5_4:65119947-65119966 Msa0864630:three_prime_UTR 45.0%
GGAACAGAAGCAACACAATC+AGG - chr5_4:65119992-65120011 None:intergenic 45.0%
GAAGAAACTGAAGCCGAAGC+TGG + chr5_4:65118434-65118453 Msa0864630:CDS 50.0%
TGTGCCCTGGTACGCTATTT+AGG + chr5_4:65118758-65118777 Msa0864630:intron 50.0%
TGCTGAGAAGATGGGTGTGT+TGG + chr5_4:65118915-65118934 Msa0864630:intron 50.0%
CGTCGTCGTTGTAGTTTGAG+TGG - chr5_4:65119757-65119776 None:intergenic 50.0%
GCCATAAGCCAAGACTCAGA+AGG + chr5_4:65119848-65119867 Msa0864630:three_prime_UTR 50.0%
!! GGGCCAGCTGTTTTATGCAA+TGG + chr5_4:65119873-65119892 Msa0864630:three_prime_UTR 50.0%
GAAGAAACTGAAGCCGAAGC+TGG + chr5_4:65118434-65118453 Msa0864630:CDS 50.0%
TGTGCCCTGGTACGCTATTT+AGG + chr5_4:65118758-65118777 Msa0864630:intron 50.0%
TGCTGAGAAGATGGGTGTGT+TGG + chr5_4:65118915-65118934 Msa0864630:intron 50.0%
CGTCGTCGTTGTAGTTTGAG+TGG - chr5_4:65119757-65119776 None:intergenic 50.0%
GCCATAAGCCAAGACTCAGA+AGG + chr5_4:65119848-65119867 Msa0864630:three_prime_UTR 50.0%
!! GGGCCAGCTGTTTTATGCAA+TGG + chr5_4:65119873-65119892 Msa0864630:three_prime_UTR 50.0%
TGGCCCATCCTTCTGAGTCT+TGG - chr5_4:65119859-65119878 None:intergenic 55.0%
TGGCCCATCCTTCTGAGTCT+TGG - chr5_4:65119859-65119878 None:intergenic 55.0%
ACTGAAGCCGAAGCTGGGAG+CGG + chr5_4:65118440-65118459 Msa0864630:CDS 60.0%
AGCGTTACCGCTCCCAGCTT+CGG - chr5_4:65118450-65118469 None:intergenic 60.0%
ACTGAAGCCGAAGCTGGGAG+CGG + chr5_4:65118440-65118459 Msa0864630:CDS 60.0%
AGCGTTACCGCTCCCAGCTT+CGG - chr5_4:65118450-65118469 None:intergenic 60.0%
GGTAACGCTGACAACGCCGC+CGG + chr5_4:65118461-65118480 Msa0864630:CDS 65.0%
GGTAACGCTGACAACGCCGC+CGG + chr5_4:65118461-65118480 Msa0864630:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 65118174 65120208 65118174 ID=Msa0864630;Name=Msa0864630
chr5_4 mRNA 65118174 65120208 65118174 ID=Msa0864630-mRNA-1;Parent=Msa0864630;Name=Msa0864630-mRNA-1;_AED=0.07;_eAED=0.07;_QI=112|1|1|1|0.5|0.33|3|378|130
chr5_4 exon 65118174 65118228 65118174 ID=Msa0864630-mRNA-1:exon:20548;Parent=Msa0864630-mRNA-1
chr5_4 exon 65118348 65118572 65118348 ID=Msa0864630-mRNA-1:exon:20549;Parent=Msa0864630-mRNA-1
chr5_4 exon 65119606 65120208 65119606 ID=Msa0864630-mRNA-1:exon:20550;Parent=Msa0864630-mRNA-1
chr5_4 five_prime_UTR 65118174 65118228 65118174 ID=Msa0864630-mRNA-1:five_prime_utr;Parent=Msa0864630-mRNA-1
chr5_4 five_prime_UTR 65118348 65118404 65118348 ID=Msa0864630-mRNA-1:five_prime_utr;Parent=Msa0864630-mRNA-1
chr5_4 CDS 65118405 65118572 65118405 ID=Msa0864630-mRNA-1:cds;Parent=Msa0864630-mRNA-1
chr5_4 CDS 65119606 65119830 65119606 ID=Msa0864630-mRNA-1:cds;Parent=Msa0864630-mRNA-1
chr5_4 three_prime_UTR 65119831 65120208 65119831 ID=Msa0864630-mRNA-1:three_prime_utr;Parent=Msa0864630-mRNA-1
Gene Sequence

>Msa0864630

ATGTTTGGAACAGCGATTAGAAATGCAGCGAAGAAACTGAAGCCGAAGCTGGGAGCGGTAACGCTGACAACGCCGCCGGAACAGAGACAAACAATAACAAGAGCTCTCTTTGATATTGTTAAGGAACATGGTCCTATCACCGTTTCTAATACCTGGGAACGTGTCAAGGAAGTTGGATTGAAAGATCTGACAAGCAAGAATCACATGAAAGTAGTTTTGAGATGGATGAGAGAGAGACAGAAGCTTCGTCTTGTTTGCAACCATGTTGGAGCTCATAAACAGTTTCTCTATACCACTTGGTTTACCAAACCAGGTACCACTCAAACTACAACGACGACGACAACAACAACAACGAAAAGCATTCCTTCAAAGAAAAGGCTAGCACGATCTTGA
Protein sequence

>Msa0864630

MFGTAIRNAAKKLKPKLGAVTLTTPPEQRQTITRALFDIVKEHGPITVSNTWERVKEVGLKDLTSKNHMKVVLRWMRERQKLRLVCNHVGAHKQFLYTTWFTKPGTTQTTTTTTTTTTKSIPSKKRLARS*