AlfalfaGEDB | Msa0864630

M. sativa cultivar zhongmu-4 / Msa0864630

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0864630	XP_003617944.1	100.000	130	0	0	1	130	1	130	1.29e-88	263

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0864630	G7KEM8	100.000	130	0	0	1	130	1	130	6.15e-89	263

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0255690	Msa0864630	0.807100	5.959961e-50	-8.615850e-47
Msa0277400	Msa0864630	0.816355	5.772211e-52	-8.615850e-47
Msa0388160	Msa0864630	0.973311	3.255574e-136	-8.615850e-47
Msa0652400	Msa0864630	0.804752	1.857841e-49	-8.615850e-47
Msa0788410	Msa0864630	0.972619	4.605758e-135	-8.615850e-47
Msa0826580	Msa0864630	0.907373	5.434582e-81	-8.615850e-47
Msa0864630	Msa1433710	0.800944	1.136269e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0864630	MtrunA17_Chr5g0447491	100.000	130	0	0	1	130	1	130	1.18e-92	263

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0864630	AT5G49410.1	69.307	101	31	0	3	103	18	118	2.55e-51	159
Msa0864630	AT5G49410.2	69.307	101	31	0	3	103	18	118	2.55e-51	159
Msa0864630	AT1G73940.1	37.069	116	64	2	11	126	43	149	3.73e-23	88.6

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Find 48 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCATTCCTTCAAAGAAA+AGG	0.466561	5_4:+65119793	Msa0864630:CDS
AACAGTTTCTCTATACCACT+TGG	0.548939	5_4:+65119715	Msa0864630:CDS
AAGAAACTGAAGCCGAAGCT+GGG	0.553771	5_4:+65118435	Msa0864630:CDS
AAGCCAAGACTCAGAAGGAT+GGG	0.568171	5_4:+65119853	Msa0864630:three_prime_UTR
AATCTGAATACTGGGCCATA+TGG	0.489508	5_4:+65119943	Msa0864630:three_prime_UTR
AATTACCTTGACACGTTCCC+AGG	0.464678	5_4:-65118556	None:intergenic
AATTAGAGTAGTGAGAAATT+AGG	0.247656	5_4:+65118368	Msa0864630:five_prime_UTR
ACCACTTGGTTTACCAAACC+AGG	0.536017	5_4:+65119729	Msa0864630:CDS
ACCTGGTTTGGTAAACCAAG+TGG	0.666171	5_4:-65119730	None:intergenic
ACGTTCCCAGGTATTAGAAA+CGG	0.576633	5_4:-65118544	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGCTCTCTTTGATATTGTTA+AGG	+	chr5_4:65118506-65118525	Msa0864630:CDS	30.0%
AGGACACATAAGAATTTACA+TGG	+	chr5_4:65118976-65118995	Msa0864630:intron	30.0%
TAGAATCTTGAAAACTTGTG+TGG	+	chr5_4:65119063-65119082	Msa0864630:intron	30.0%
GATTACTACTCAAGTTTACA+GGG	-	chr5_4:65119091-65119110	None:intergenic	30.0%
ATACAAACAAAATCACGTGA+AGG	-	chr5_4:65119216-65119235	None:intergenic	30.0%
GAAAATTGTTACTCTGTTGA+AGG	+	chr5_4:65119316-65119335	Msa0864630:intron	30.0%
TTACATATCTGTGCATCTAT+TGG	+	chr5_4:65119537-65119556	Msa0864630:intron	30.0%
AACTTCCTGGAAAAAATTGA+AGG	-	chr5_4:65119592-65119611	None:intergenic	30.0%
TTATGCAATGGGAAGAATAA+TGG	+	chr5_4:65119885-65119904	Msa0864630:three_prime_UTR	30.0%
CAATGAAGAATCTGAATACT+GGG	+	chr5_4:65119935-65119954	Msa0864630:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	65118174	65120208	65118174	ID=Msa0864630;Name=Msa0864630
chr5_4	mRNA	65118174	65120208	65118174	ID=Msa0864630-mRNA-1;Parent=Msa0864630;Name=Msa0864630-mRNA-1;_AED=0.07;_eAED=0.07;_QI=112\|1\|1\|1\|0.5\|0.33\|3\|378\|130
chr5_4	exon	65118174	65118228	65118174	ID=Msa0864630-mRNA-1:exon:20548;Parent=Msa0864630-mRNA-1
chr5_4	exon	65118348	65118572	65118348	ID=Msa0864630-mRNA-1:exon:20549;Parent=Msa0864630-mRNA-1
chr5_4	exon	65119606	65120208	65119606	ID=Msa0864630-mRNA-1:exon:20550;Parent=Msa0864630-mRNA-1
chr5_4	five_prime_UTR	65118174	65118228	65118174	ID=Msa0864630-mRNA-1:five_prime_utr;Parent=Msa0864630-mRNA-1
chr5_4	five_prime_UTR	65118348	65118404	65118348	ID=Msa0864630-mRNA-1:five_prime_utr;Parent=Msa0864630-mRNA-1
chr5_4	CDS	65118405	65118572	65118405	ID=Msa0864630-mRNA-1:cds;Parent=Msa0864630-mRNA-1
chr5_4	CDS	65119606	65119830	65119606	ID=Msa0864630-mRNA-1:cds;Parent=Msa0864630-mRNA-1
chr5_4	three_prime_UTR	65119831	65120208	65119831	ID=Msa0864630-mRNA-1:three_prime_utr;Parent=Msa0864630-mRNA-1

Gene Sequence

>Msa0864630

ATGTTTGGAACAGCGATTAGAAATGCAGCGAAGAAACTGAAGCCGAAGCTGGGAGCGGTAACGCTGACAACGCCGCCGGAACAGAGACAAACAATAACAAGAGCTCTCTTTGATATTGTTAAGGAACATGGTCCTATCACCGTTTCTAATACCTGGGAACGTGTCAAGGAAGTTGGATTGAAAGATCTGACAAGCAAGAATCACATGAAAGTAGTTTTGAGATGGATGAGAGAGAGACAGAAGCTTCGTCTTGTTTGCAACCATGTTGGAGCTCATAAACAGTTTCTCTATACCACTTGGTTTACCAAACCAGGTACCACTCAAACTACAACGACGACGACAACAACAACAACGAAAAGCATTCCTTCAAAGAAAAGGCTAGCACGATCTTGA

Protein sequence

>Msa0864630

MFGTAIRNAAKKLKPKLGAVTLTTPPEQRQTITRALFDIVKEHGPITVSNTWERVKEVGLKDLTSKNHMKVVLRWMRERQKLRLVCNHVGAHKQFLYTTWFTKPGTTQTTTTTTTTTTKSIPSKKRLARS*