AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0652400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0652400 MtrunA17_Chr4g0045981 98.370 184 3 0 1 184 446 629 3.90e-133 386
Msa0652400 MtrunA17_Chr1g0151531 63.253 166 58 1 1 163 583 748 4.22e-66 215
Msa0652400 MtrunA17_Chr5g0422391 59.524 168 65 1 1 165 814 981 7.53e-63 209
Msa0652400 MtrunA17_Chr1g0198861 59.064 171 64 2 2 169 803 970 3.03e-59 199
Msa0652400 MtrunA17_Chr5g0430701 51.235 162 77 2 1 160 256 417 1.76e-52 173
Msa0652400 MtrunA17_Chr4g0069431 47.561 164 83 1 2 162 393 556 9.27e-47 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0652400 AT4G18570.1 77.540 187 39 2 1 184 456 642 3.26e-90 276
Msa0652400 AT3G25690.3 63.095 168 59 1 1 165 676 843 8.59e-67 219
Msa0652400 AT3G25690.1 63.095 168 59 1 1 165 817 984 3.02e-66 219
Msa0652400 AT3G25690.2 63.095 168 59 1 1 165 817 984 3.02e-66 219
Msa0652400 AT3G25690.6 63.095 168 59 1 1 165 817 984 3.02e-66 219
Msa0652400 AT3G25690.4 63.095 168 59 1 1 165 817 984 3.02e-66 219
Msa0652400 AT3G25690.5 63.095 168 59 1 1 165 819 986 3.18e-66 218
Msa0652400 AT1G07120.1 50.311 161 78 2 1 160 224 383 2.78e-52 172
Msa0652400 AT1G48280.1 50.299 167 80 1 2 165 378 544 2.37e-49 167
Msa0652400 AT4G18570.2 74.747 99 25 0 1 99 456 554 1.60e-46 160
Msa0652400 AT1G07120.2 49.000 100 50 1 1 99 224 323 6.27e-27 104

Find 50 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATACTAATTTAATTATTTC+TGG 0.099407 4_3:+60118154 None:intergenic
TATTCACAAACTAGTGAAAA+AGG 0.219685 4_3:+60118126 None:intergenic
TGGCTTTATCTCTCAATTCT+TGG 0.255039 4_3:+60118260 None:intergenic
GCAACCAATCTACAGGTATT+TGG 0.272044 4_3:+60119250 None:intergenic
AAAGCTGGCATCTGTAAAAT+TGG 0.288625 4_3:-60118550 Msa0652400:CDS
TCACTAGTTTGTGAATATAA+TGG 0.291207 4_3:-60118120 Msa0652400:three_prime_UTR
ACAATGAGCTCTTCTTCTTC+AGG 0.305449 4_3:+60118474 None:intergenic
GCTTGTTCTTGTGAATTATC+TGG 0.328841 4_3:-60117994 Msa0652400:three_prime_UTR
TCATTCATTTATAAAGAAAC+AGG 0.406634 4_3:-60117951 Msa0652400:three_prime_UTR
GCTATGCGTGAAGCGGCATT+TGG 0.410148 4_3:-60119738 Msa0652400:CDS
AATGTATTCCACTTTAATGA+TGG 0.417417 4_3:+60118082 None:intergenic
CGGTGCTCAAACACTTTGAT+TGG 0.422359 4_3:-60119779 Msa0652400:CDS
ATTTGCTTTCCGTGTACATC+AGG 0.429708 4_3:-60118439 Msa0652400:intron
TCTTCCAAATACCTGTAGAT+TGG 0.435132 4_3:-60119254 Msa0652400:CDS
GTCTGCATCTTCTTTAGAGC+AGG 0.439557 4_3:+60119645 None:intergenic
AAATTAATTTGCTTCCACTT+TGG 0.442448 4_3:-60118018 Msa0652400:three_prime_UTR
GAGCTTGAAACTGTTGGTGG+TGG 0.454165 4_3:-60118498 Msa0652400:CDS
CTTAATTAAAATTACCTTGG+TGG 0.459160 4_3:+60119212 None:intergenic
CGTCTTTCCGCGACGATCCT+CGG 0.464534 4_3:-60119680 Msa0652400:CDS
AGCTTGAAACTGTTGGTGGT+GGG 0.479377 4_3:-60118497 Msa0652400:CDS
ATAGCATCAGCCTTCTGCTC+AGG 0.484971 4_3:+60119756 None:intergenic
CTTTGATTGGCCTGAGCAGA+AGG 0.487775 4_3:-60119766 Msa0652400:CDS
TCTGCTGAGCTTGAAACTGT+TGG 0.494822 4_3:-60118504 Msa0652400:CDS
GGAAAGCAAATCTGACTCCT+TGG 0.497658 4_3:+60118451 None:intergenic
ACGCTTAGCCATCATTAAAG+TGG 0.504308 4_3:-60118090 Msa0652400:three_prime_UTR
TTGCTCCTGCATTAACATGC+TGG 0.514086 4_3:+60118184 None:intergenic
AAGAATTGAGAGATAAAGCC+AGG 0.519809 4_3:-60118258 Msa0652400:CDS
GCTGAGCTTGAAACTGTTGG+TGG 0.524244 4_3:-60118501 Msa0652400:CDS
GGCCTCCAGCATGTTAATGC+AGG 0.528236 4_3:-60118189 Msa0652400:exon
CTATCAAGCAACCAATCTAC+AGG 0.536269 4_3:+60119243 None:intergenic
AATTGTGTTATAGATAAAGC+TGG 0.545820 4_3:-60118565 Msa0652400:intron
TCATGTCATGTTCAATGCCA+TGG 0.550049 4_3:-60118235 Msa0652400:CDS
GAAGAAGAGCTCATTGTCCA+AGG 0.553034 4_3:-60118468 Msa0652400:CDS
CACAAGAACAAGCGCCAAAG+TGG 0.567622 4_3:+60118004 None:intergenic
CTCCTGCATTAACATGCTGG+AGG 0.576851 4_3:+60118187 None:intergenic
GGCTGATGCTATGCGTGAAG+CGG 0.578357 4_3:-60119745 Msa0652400:CDS
GTTGCAACAGATTAGAACAT+GGG 0.581672 4_3:-60119326 Msa0652400:intron
GTAGATTGGTTGCTTGATAG+CGG 0.595636 4_3:-60119240 Msa0652400:CDS
TTACTGATTTGTTTAACGAA+TGG 0.596856 4_3:+60118043 None:intergenic
GTCGCGGAAAGACGAAGCCT+CGG 0.598289 4_3:+60119689 None:intergenic
GCAGGACTACAGAGCTGCCG+AGG 0.616236 4_3:+60119663 None:intergenic
GATGATGGTGCAATTGTCCA+TGG 0.632629 4_3:+60117842 None:intergenic
TTGCAACAGATTAGAACATG+GGG 0.633670 4_3:-60119325 Msa0652400:intron
TGTTGCAACAGATTAGAACA+TGG 0.634846 4_3:-60119327 Msa0652400:intron
TCTGAAGAAACTAGAATCCG+AGG 0.653972 4_3:-60119706 Msa0652400:CDS
GCATTGAACATGACATGACC+TGG 0.668619 4_3:+60118240 None:intergenic
TCATTTATAAAGAAACAGGA+CGG 0.688304 4_3:-60117947 Msa0652400:three_prime_UTR
TGATAGCGGTTATGCCACCA+AGG 0.695302 4_3:-60119226 Msa0652400:intron
AGAGCTGCCGAGGATCGTCG+CGG 0.705530 4_3:+60119673 None:intergenic
GTTATAGGTGGATGAAAGAG+CGG 0.726818 4_3:-60119799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAACTTTTAATAAAGTT+AGG + chr4_3:60118201-60118220 None:intergenic 10.0%
!! TAAATATGAAACAAAAAAAA+AGG + chr4_3:60118996-60119015 None:intergenic 10.0%
!! TATACTAATTTAATTATTTC+TGG + chr4_3:60119492-60119511 None:intergenic 10.0%
!!! TATTAACTTTTAATAAAGTT+AGG + chr4_3:60118201-60118220 None:intergenic 10.0%
!! TAAATATGAAACAAAAAAAA+AGG + chr4_3:60118996-60119015 None:intergenic 10.0%
!! TATACTAATTTAATTATTTC+TGG + chr4_3:60119492-60119511 None:intergenic 10.0%
!! AAAATAATAAACAGTGATAA+AGG + chr4_3:60118459-60118478 None:intergenic 15.0%
!!! AATGAATTGATGTTATTTAT+TGG - chr4_3:60118561-60118580 Msa0652400:intron 15.0%
!!! AATTTTAAAGATAAGCATAT+AGG + chr4_3:60118930-60118949 None:intergenic 15.0%
!!! TTTTGTTTCATATTTAATCA+CGG - chr4_3:60119000-60119019 Msa0652400:intron 15.0%
!! AAAATAATAAACAGTGATAA+AGG + chr4_3:60118459-60118478 None:intergenic 15.0%
!!! AATGAATTGATGTTATTTAT+TGG - chr4_3:60118561-60118580 Msa0652400:intron 15.0%
!!! AATTTTAAAGATAAGCATAT+AGG + chr4_3:60118930-60118949 None:intergenic 15.0%
!!! TTTTGTTTCATATTTAATCA+CGG - chr4_3:60119000-60119019 Msa0652400:intron 15.0%
!!! TTTAGCTAGTATCTTATTTA+AGG - chr4_3:60118063-60118082 Msa0652400:three_prime_UTR 20.0%
!! TAAGGTTTCATAATTCATAT+TGG - chr4_3:60118081-60118100 Msa0652400:three_prime_UTR 20.0%
!! TGCAACATTTAAAAACAAAA+GGG + chr4_3:60118303-60118322 None:intergenic 20.0%
!! TTGCAACATTTAAAAACAAA+AGG + chr4_3:60118304-60118323 None:intergenic 20.0%
!! GAACTTAATTAAAATTACCT+TGG + chr4_3:60118437-60118456 None:intergenic 20.0%
!!! TCAACCAGTTTATTATATTT+TGG - chr4_3:60118527-60118546 Msa0652400:CDS 20.0%
!! TGATCCAAAATATAATAAAC+TGG + chr4_3:60118534-60118553 None:intergenic 20.0%
!! GAAGATTAATATTTACGAAA+GGG + chr4_3:60118602-60118621 None:intergenic 20.0%
!! AGAAGATTAATATTTACGAA+AGG + chr4_3:60118603-60118622 None:intergenic 20.0%
!!! AAGGGAAACTATATTTTATA+AGG - chr4_3:60118888-60118907 Msa0652400:intron 20.0%
!! TCATTCATTTATAAAGAAAC+AGG - chr4_3:60119692-60119711 Msa0652400:CDS 20.0%
!!! TTTAGCTAGTATCTTATTTA+AGG - chr4_3:60118063-60118082 Msa0652400:three_prime_UTR 20.0%
!! TAAGGTTTCATAATTCATAT+TGG - chr4_3:60118081-60118100 Msa0652400:three_prime_UTR 20.0%
!! TGCAACATTTAAAAACAAAA+GGG + chr4_3:60118303-60118322 None:intergenic 20.0%
!! TTGCAACATTTAAAAACAAA+AGG + chr4_3:60118304-60118323 None:intergenic 20.0%
!! GAACTTAATTAAAATTACCT+TGG + chr4_3:60118437-60118456 None:intergenic 20.0%
!!! TCAACCAGTTTATTATATTT+TGG - chr4_3:60118527-60118546 Msa0652400:CDS 20.0%
!! TGATCCAAAATATAATAAAC+TGG + chr4_3:60118534-60118553 None:intergenic 20.0%
!! GAAGATTAATATTTACGAAA+GGG + chr4_3:60118602-60118621 None:intergenic 20.0%
!! AGAAGATTAATATTTACGAA+AGG + chr4_3:60118603-60118622 None:intergenic 20.0%
!!! AAGGGAAACTATATTTTATA+AGG - chr4_3:60118888-60118907 Msa0652400:intron 20.0%
!! TCATTCATTTATAAAGAAAC+AGG - chr4_3:60119692-60119711 Msa0652400:CDS 20.0%
!!! GCATTCATTTTAATGAAGTT+AGG + chr4_3:60118125-60118144 None:intergenic 25.0%
! CTTAATTAAAATTACCTTGG+TGG + chr4_3:60118434-60118453 None:intergenic 25.0%
!! ATTTATTGGTTGATGTTGAA+TGG - chr4_3:60118575-60118594 Msa0652400:intron 25.0%
!! TTTATTGGTTGATGTTGAAT+GGG - chr4_3:60118576-60118595 Msa0652400:intron 25.0%
! AATTGTGTTATAGATAAAGC+TGG - chr4_3:60119078-60119097 Msa0652400:intron 25.0%
!! AAATTTTTACCTGATGTACA+CGG + chr4_3:60119216-60119235 None:intergenic 25.0%
!!! GAGTAATGTGCTATTATTTT+TGG - chr4_3:60119319-60119338 Msa0652400:CDS 25.0%
! ATTCACAAACTAGTGAAAAA+GGG + chr4_3:60119519-60119538 None:intergenic 25.0%
! TATTCACAAACTAGTGAAAA+AGG + chr4_3:60119520-60119539 None:intergenic 25.0%
!! TCACTAGTTTGTGAATATAA+TGG - chr4_3:60119523-60119542 Msa0652400:intron 25.0%
! AATGTATTCCACTTTAATGA+TGG + chr4_3:60119564-60119583 None:intergenic 25.0%
!!! TTACTGATTTGTTTAACGAA+TGG + chr4_3:60119603-60119622 None:intergenic 25.0%
!!! AAATTAATTTGCTTCCACTT+TGG - chr4_3:60119625-60119644 Msa0652400:intron 25.0%
! TCATTTATAAAGAAACAGGA+CGG - chr4_3:60119696-60119715 Msa0652400:CDS 25.0%
!!! TTGTTTATTTTCATTTGCCA+TGG - chr4_3:60119784-60119803 Msa0652400:CDS 25.0%
!!! GCATTCATTTTAATGAAGTT+AGG + chr4_3:60118125-60118144 None:intergenic 25.0%
! CTTAATTAAAATTACCTTGG+TGG + chr4_3:60118434-60118453 None:intergenic 25.0%
!! ATTTATTGGTTGATGTTGAA+TGG - chr4_3:60118575-60118594 Msa0652400:intron 25.0%
!! TTTATTGGTTGATGTTGAAT+GGG - chr4_3:60118576-60118595 Msa0652400:intron 25.0%
! AATTGTGTTATAGATAAAGC+TGG - chr4_3:60119078-60119097 Msa0652400:intron 25.0%
!! AAATTTTTACCTGATGTACA+CGG + chr4_3:60119216-60119235 None:intergenic 25.0%
!!! GAGTAATGTGCTATTATTTT+TGG - chr4_3:60119319-60119338 Msa0652400:CDS 25.0%
! ATTCACAAACTAGTGAAAAA+GGG + chr4_3:60119519-60119538 None:intergenic 25.0%
! TATTCACAAACTAGTGAAAA+AGG + chr4_3:60119520-60119539 None:intergenic 25.0%
!! TCACTAGTTTGTGAATATAA+TGG - chr4_3:60119523-60119542 Msa0652400:intron 25.0%
! AATGTATTCCACTTTAATGA+TGG + chr4_3:60119564-60119583 None:intergenic 25.0%
!!! TTACTGATTTGTTTAACGAA+TGG + chr4_3:60119603-60119622 None:intergenic 25.0%
!!! AAATTAATTTGCTTCCACTT+TGG - chr4_3:60119625-60119644 Msa0652400:intron 25.0%
! TCATTTATAAAGAAACAGGA+CGG - chr4_3:60119696-60119715 Msa0652400:CDS 25.0%
!!! TTGTTTATTTTCATTTGCCA+TGG - chr4_3:60119784-60119803 Msa0652400:CDS 25.0%
TTTGAGTACTATAGACAAGT+TGG + chr4_3:60118641-60118660 None:intergenic 30.0%
TGCTATAAGAAACTTTACCA+TGG + chr4_3:60118671-60118690 None:intergenic 30.0%
TAATAGATTGTGGAGATCTT+GGG + chr4_3:60118815-60118834 None:intergenic 30.0%
TTTCATATTTAATCACGGTG+AGG - chr4_3:60119005-60119024 Msa0652400:intron 30.0%
TGAGGTGAAATCTCAAATAT+TGG - chr4_3:60119023-60119042 Msa0652400:intron 30.0%
TTTGAGTACTATAGACAAGT+TGG + chr4_3:60118641-60118660 None:intergenic 30.0%
TGCTATAAGAAACTTTACCA+TGG + chr4_3:60118671-60118690 None:intergenic 30.0%
TAATAGATTGTGGAGATCTT+GGG + chr4_3:60118815-60118834 None:intergenic 30.0%
TTTCATATTTAATCACGGTG+AGG - chr4_3:60119005-60119024 Msa0652400:intron 30.0%
TGAGGTGAAATCTCAAATAT+TGG - chr4_3:60119023-60119042 Msa0652400:intron 30.0%
TGTTGCAACAGATTAGAACA+TGG - chr4_3:60118316-60118335 Msa0652400:intron 35.0%
GTTGCAACAGATTAGAACAT+GGG - chr4_3:60118317-60118336 Msa0652400:intron 35.0%
TTGCAACAGATTAGAACATG+GGG - chr4_3:60118318-60118337 Msa0652400:intron 35.0%
TCTTCCAAATACCTGTAGAT+TGG - chr4_3:60118389-60118408 Msa0652400:intron 35.0%
! GTTTTCACGAACGAGAAAAA+TGG - chr4_3:60118486-60118505 Msa0652400:CDS 35.0%
TGCTGATAAATTTCATCCTC+TGG - chr4_3:60118743-60118762 Msa0652400:intron 35.0%
ATTGAAAACACAGAAACCAG+AGG + chr4_3:60118762-60118781 None:intergenic 35.0%
AGATCTTGGGTATCTATCTA+GGG + chr4_3:60118802-60118821 None:intergenic 35.0%
CTAATAGATTGTGGAGATCT+TGG + chr4_3:60118816-60118835 None:intergenic 35.0%
CAAGATCTCCACAATCTATT+AGG - chr4_3:60118814-60118833 Msa0652400:intron 35.0%
CATACAGTCCTAATAGATTG+TGG + chr4_3:60118825-60118844 None:intergenic 35.0%
AGAAGGAGATTCAACTTTGA+AGG - chr4_3:60118869-60118888 Msa0652400:intron 35.0%
GAAGGAGATTCAACTTTGAA+GGG - chr4_3:60118870-60118889 Msa0652400:intron 35.0%
AAAGCTGGCATCTGTAAAAT+TGG - chr4_3:60119093-60119112 Msa0652400:intron 35.0%
TGCCTATACAACCATTGTAA+AGG - chr4_3:60119266-60119285 Msa0652400:CDS 35.0%
GTCCTTTACAATGGTTGTAT+AGG + chr4_3:60119271-60119290 None:intergenic 35.0%
TAATAGCACATTACTCAGAG+TGG + chr4_3:60119315-60119334 None:intergenic 35.0%
!!! TATTTTTGGCAGTTTGCTAG+CGG - chr4_3:60119333-60119352 Msa0652400:intron 35.0%
!! TGGCTTTATCTCTCAATTCT+TGG + chr4_3:60119386-60119405 None:intergenic 35.0%
AAGAATTGAGAGATAAAGCC+AGG - chr4_3:60119385-60119404 Msa0652400:intron 35.0%
! TGCACAAGAATTTTTGTTGC+TGG + chr4_3:60119434-60119453 None:intergenic 35.0%
AGCAACAAAAATTCTTGTGC+AGG - chr4_3:60119433-60119452 Msa0652400:intron 35.0%
GCTTGTTCTTGTGAATTATC+TGG - chr4_3:60119649-60119668 Msa0652400:CDS 35.0%
TGTTGCAACAGATTAGAACA+TGG - chr4_3:60118316-60118335 Msa0652400:intron 35.0%
GTTGCAACAGATTAGAACAT+GGG - chr4_3:60118317-60118336 Msa0652400:intron 35.0%
TTGCAACAGATTAGAACATG+GGG - chr4_3:60118318-60118337 Msa0652400:intron 35.0%
TCTTCCAAATACCTGTAGAT+TGG - chr4_3:60118389-60118408 Msa0652400:intron 35.0%
! GTTTTCACGAACGAGAAAAA+TGG - chr4_3:60118486-60118505 Msa0652400:CDS 35.0%
TGCTGATAAATTTCATCCTC+TGG - chr4_3:60118743-60118762 Msa0652400:intron 35.0%
ATTGAAAACACAGAAACCAG+AGG + chr4_3:60118762-60118781 None:intergenic 35.0%
AGATCTTGGGTATCTATCTA+GGG + chr4_3:60118802-60118821 None:intergenic 35.0%
CTAATAGATTGTGGAGATCT+TGG + chr4_3:60118816-60118835 None:intergenic 35.0%
CAAGATCTCCACAATCTATT+AGG - chr4_3:60118814-60118833 Msa0652400:intron 35.0%
CATACAGTCCTAATAGATTG+TGG + chr4_3:60118825-60118844 None:intergenic 35.0%
AGAAGGAGATTCAACTTTGA+AGG - chr4_3:60118869-60118888 Msa0652400:intron 35.0%
GAAGGAGATTCAACTTTGAA+GGG - chr4_3:60118870-60118889 Msa0652400:intron 35.0%
AAAGCTGGCATCTGTAAAAT+TGG - chr4_3:60119093-60119112 Msa0652400:intron 35.0%
TGCCTATACAACCATTGTAA+AGG - chr4_3:60119266-60119285 Msa0652400:CDS 35.0%
GTCCTTTACAATGGTTGTAT+AGG + chr4_3:60119271-60119290 None:intergenic 35.0%
TAATAGCACATTACTCAGAG+TGG + chr4_3:60119315-60119334 None:intergenic 35.0%
!!! TATTTTTGGCAGTTTGCTAG+CGG - chr4_3:60119333-60119352 Msa0652400:intron 35.0%
!! TGGCTTTATCTCTCAATTCT+TGG + chr4_3:60119386-60119405 None:intergenic 35.0%
AAGAATTGAGAGATAAAGCC+AGG - chr4_3:60119385-60119404 Msa0652400:intron 35.0%
! TGCACAAGAATTTTTGTTGC+TGG + chr4_3:60119434-60119453 None:intergenic 35.0%
AGCAACAAAAATTCTTGTGC+AGG - chr4_3:60119433-60119452 Msa0652400:intron 35.0%
GCTTGTTCTTGTGAATTATC+TGG - chr4_3:60119649-60119668 Msa0652400:CDS 35.0%
TCTGAAGAAACTAGAATCCG+AGG - chr4_3:60117937-60117956 Msa0652400:three_prime_UTR 40.0%
GCAACCAATCTACAGGTATT+TGG + chr4_3:60118396-60118415 None:intergenic 40.0%
CTATCAAGCAACCAATCTAC+AGG + chr4_3:60118403-60118422 None:intergenic 40.0%
! GTAGATTGGTTGCTTGATAG+CGG - chr4_3:60118403-60118422 Msa0652400:intron 40.0%
AGTACTCAAAACAGTAGCCA+TGG - chr4_3:60118651-60118670 Msa0652400:intron 40.0%
GAGATCTTGGGTATCTATCT+AGG + chr4_3:60118803-60118822 None:intergenic 40.0%
!! CTGCTTGGTCTTTTTAGAGA+AGG - chr4_3:60118852-60118871 Msa0652400:intron 40.0%
ACAATGAGCTCTTCTTCTTC+AGG + chr4_3:60119172-60119191 None:intergenic 40.0%
ATTTGCTTTCCGTGTACATC+AGG - chr4_3:60119204-60119223 Msa0652400:intron 40.0%
CTCAGAATGGTCCTTTACAA+TGG + chr4_3:60119280-60119299 None:intergenic 40.0%
GACCATTCTGAGCAATGTAA+AGG - chr4_3:60119288-60119307 Msa0652400:CDS 40.0%
GTCCTTTACATTGCTCAGAA+TGG + chr4_3:60119293-60119312 None:intergenic 40.0%
TCATGTCATGTTCAATGCCA+TGG - chr4_3:60119408-60119427 Msa0652400:intron 40.0%
! AGAATTTTTGTTGCTGGCCA+TGG + chr4_3:60119428-60119447 None:intergenic 40.0%
ACGCTTAGCCATCATTAAAG+TGG - chr4_3:60119553-60119572 Msa0652400:intron 40.0%
TCTGAAGAAACTAGAATCCG+AGG - chr4_3:60117937-60117956 Msa0652400:three_prime_UTR 40.0%
GCAACCAATCTACAGGTATT+TGG + chr4_3:60118396-60118415 None:intergenic 40.0%
CTATCAAGCAACCAATCTAC+AGG + chr4_3:60118403-60118422 None:intergenic 40.0%
! GTAGATTGGTTGCTTGATAG+CGG - chr4_3:60118403-60118422 Msa0652400:intron 40.0%
AGTACTCAAAACAGTAGCCA+TGG - chr4_3:60118651-60118670 Msa0652400:intron 40.0%
GAGATCTTGGGTATCTATCT+AGG + chr4_3:60118803-60118822 None:intergenic 40.0%
!! CTGCTTGGTCTTTTTAGAGA+AGG - chr4_3:60118852-60118871 Msa0652400:intron 40.0%
ACAATGAGCTCTTCTTCTTC+AGG + chr4_3:60119172-60119191 None:intergenic 40.0%
ATTTGCTTTCCGTGTACATC+AGG - chr4_3:60119204-60119223 Msa0652400:intron 40.0%
CTCAGAATGGTCCTTTACAA+TGG + chr4_3:60119280-60119299 None:intergenic 40.0%
GACCATTCTGAGCAATGTAA+AGG - chr4_3:60119288-60119307 Msa0652400:CDS 40.0%
GTCCTTTACATTGCTCAGAA+TGG + chr4_3:60119293-60119312 None:intergenic 40.0%
TCATGTCATGTTCAATGCCA+TGG - chr4_3:60119408-60119427 Msa0652400:intron 40.0%
! AGAATTTTTGTTGCTGGCCA+TGG + chr4_3:60119428-60119447 None:intergenic 40.0%
ACGCTTAGCCATCATTAAAG+TGG - chr4_3:60119553-60119572 Msa0652400:intron 40.0%
CGGTGCTCAAACACTTTGAT+TGG - chr4_3:60117864-60117883 Msa0652400:three_prime_UTR 45.0%
!! GTCTGCATCTTCTTTAGAGC+AGG + chr4_3:60118001-60118020 None:intergenic 45.0%
ACTGTATGCTTGTGTCTGCT+TGG - chr4_3:60118837-60118856 Msa0652400:intron 45.0%
TCTGCTGAGCTTGAAACTGT+TGG - chr4_3:60119139-60119158 Msa0652400:intron 45.0%
!! AGCTTGAAACTGTTGGTGGT+GGG - chr4_3:60119146-60119165 Msa0652400:intron 45.0%
GAAGAAGAGCTCATTGTCCA+AGG - chr4_3:60119175-60119194 Msa0652400:intron 45.0%
GGAAAGCAAATCTGACTCCT+TGG + chr4_3:60119195-60119214 None:intergenic 45.0%
!! GTTTGCTAGCGGTTTTGATG+CGG - chr4_3:60119344-60119363 Msa0652400:intron 45.0%
GCATTGAACATGACATGACC+TGG + chr4_3:60119406-60119425 None:intergenic 45.0%
TTGCTCCTGCATTAACATGC+TGG + chr4_3:60119462-60119481 None:intergenic 45.0%
CGGTGCTCAAACACTTTGAT+TGG - chr4_3:60117864-60117883 Msa0652400:three_prime_UTR 45.0%
!! GTCTGCATCTTCTTTAGAGC+AGG + chr4_3:60118001-60118020 None:intergenic 45.0%
ACTGTATGCTTGTGTCTGCT+TGG - chr4_3:60118837-60118856 Msa0652400:intron 45.0%
TCTGCTGAGCTTGAAACTGT+TGG - chr4_3:60119139-60119158 Msa0652400:intron 45.0%
!! AGCTTGAAACTGTTGGTGGT+GGG - chr4_3:60119146-60119165 Msa0652400:intron 45.0%
GAAGAAGAGCTCATTGTCCA+AGG - chr4_3:60119175-60119194 Msa0652400:intron 45.0%
GGAAAGCAAATCTGACTCCT+TGG + chr4_3:60119195-60119214 None:intergenic 45.0%
!! GTTTGCTAGCGGTTTTGATG+CGG - chr4_3:60119344-60119363 Msa0652400:intron 45.0%
GCATTGAACATGACATGACC+TGG + chr4_3:60119406-60119425 None:intergenic 45.0%
TTGCTCCTGCATTAACATGC+TGG + chr4_3:60119462-60119481 None:intergenic 45.0%
!! CTTTGATTGGCCTGAGCAGA+AGG - chr4_3:60117877-60117896 Msa0652400:three_prime_UTR 50.0%
ATAGCATCAGCCTTCTGCTC+AGG + chr4_3:60117890-60117909 None:intergenic 50.0%
!! TGATAGCGGTTATGCCACCA+AGG - chr4_3:60118417-60118436 Msa0652400:intron 50.0%
GCTGAGCTTGAAACTGTTGG+TGG - chr4_3:60119142-60119161 Msa0652400:intron 50.0%
! GAGCTTGAAACTGTTGGTGG+TGG - chr4_3:60119145-60119164 Msa0652400:intron 50.0%
CTCCTGCATTAACATGCTGG+AGG + chr4_3:60119459-60119478 None:intergenic 50.0%
CACAAGAACAAGCGCCAAAG+TGG + chr4_3:60119642-60119661 None:intergenic 50.0%
!! CTTTGATTGGCCTGAGCAGA+AGG - chr4_3:60117877-60117896 Msa0652400:three_prime_UTR 50.0%
ATAGCATCAGCCTTCTGCTC+AGG + chr4_3:60117890-60117909 None:intergenic 50.0%
!! TGATAGCGGTTATGCCACCA+AGG - chr4_3:60118417-60118436 Msa0652400:intron 50.0%
GCTGAGCTTGAAACTGTTGG+TGG - chr4_3:60119142-60119161 Msa0652400:intron 50.0%
! GAGCTTGAAACTGTTGGTGG+TGG - chr4_3:60119145-60119164 Msa0652400:intron 50.0%
CTCCTGCATTAACATGCTGG+AGG + chr4_3:60119459-60119478 None:intergenic 50.0%
CACAAGAACAAGCGCCAAAG+TGG + chr4_3:60119642-60119661 None:intergenic 50.0%
GGCTGATGCTATGCGTGAAG+CGG - chr4_3:60117898-60117917 Msa0652400:three_prime_UTR 55.0%
GCTATGCGTGAAGCGGCATT+TGG - chr4_3:60117905-60117924 Msa0652400:three_prime_UTR 55.0%
GGCCTCCAGCATGTTAATGC+AGG - chr4_3:60119454-60119473 Msa0652400:intron 55.0%
GGCTGATGCTATGCGTGAAG+CGG - chr4_3:60117898-60117917 Msa0652400:three_prime_UTR 55.0%
GCTATGCGTGAAGCGGCATT+TGG - chr4_3:60117905-60117924 Msa0652400:three_prime_UTR 55.0%
GGCCTCCAGCATGTTAATGC+AGG - chr4_3:60119454-60119473 Msa0652400:intron 55.0%
GTCGCGGAAAGACGAAGCCT+CGG + chr4_3:60117957-60117976 None:intergenic 60.0%
CGTCTTTCCGCGACGATCCT+CGG - chr4_3:60117963-60117982 Msa0652400:three_prime_UTR 60.0%
GTCGCGGAAAGACGAAGCCT+CGG + chr4_3:60117957-60117976 None:intergenic 60.0%
CGTCTTTCCGCGACGATCCT+CGG - chr4_3:60117963-60117982 Msa0652400:three_prime_UTR 60.0%
AGAGCTGCCGAGGATCGTCG+CGG + chr4_3:60117973-60117992 None:intergenic 65.0%
GCAGGACTACAGAGCTGCCG+AGG + chr4_3:60117983-60118002 None:intergenic 65.0%
AGAGCTGCCGAGGATCGTCG+CGG + chr4_3:60117973-60117992 None:intergenic 65.0%
GCAGGACTACAGAGCTGCCG+AGG + chr4_3:60117983-60118002 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 60117851 60119814 60117851 ID=Msa0652400;Name=Msa0652400
chr4_3 mRNA 60117851 60119814 60117851 ID=Msa0652400-mRNA-1;Parent=Msa0652400;Name=Msa0652400-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.75|1|1|1|4|344|184
chr4_3 exon 60117851 60118320 60117851 ID=Msa0652400-mRNA-1:exon:16291;Parent=Msa0652400-mRNA-1
chr4_3 exon 60118440 60118574 60118440 ID=Msa0652400-mRNA-1:exon:16290;Parent=Msa0652400-mRNA-1
chr4_3 exon 60119227 60119338 60119227 ID=Msa0652400-mRNA-1:exon:16289;Parent=Msa0652400-mRNA-1
chr4_3 exon 60119633 60119814 60119633 ID=Msa0652400-mRNA-1:exon:16288;Parent=Msa0652400-mRNA-1
chr4_3 CDS 60119633 60119814 60119633 ID=Msa0652400-mRNA-1:cds;Parent=Msa0652400-mRNA-1
chr4_3 CDS 60119227 60119338 60119227 ID=Msa0652400-mRNA-1:cds;Parent=Msa0652400-mRNA-1
chr4_3 CDS 60118440 60118574 60118440 ID=Msa0652400-mRNA-1:cds;Parent=Msa0652400-mRNA-1
chr4_3 CDS 60118195 60118320 60118195 ID=Msa0652400-mRNA-1:cds;Parent=Msa0652400-mRNA-1
chr4_3 three_prime_UTR 60117851 60118194 60117851 ID=Msa0652400-mRNA-1:three_prime_utr;Parent=Msa0652400-mRNA-1
Gene Sequence

>Msa0652400

GTGGATGAAAGAGCGGTGCTCAAACACTTTGATTGGCCTGAGCAGAAGGCTGATGCTATGCGTGAAGCGGCATTTGGATATTGTGATCTGAAGAAACTAGAATCCGAGGCTTCGTCTTTCCGCGACGATCCTCGGCAGCTCTGTAGTCCTGCTCTAAAGAAGATGCAGACTCTCTTTGAGAAATTAGAACATGGGGTTTACAACATTTCTAGAATGAGAGAGTCAGCAACAAAGAGATTCAAAGTCTTCCAAATACCTGTAGATTGGTTGCTTGATAGCGGTTATGCCACCAAGATAAAGCTGGCATCTGTAAAATTGGCTATGAAGTATATGAAGAGAGTTTCTGCTGAGCTTGAAACTGTTGGTGGTGGGCCTGAAGAAGAAGAGCTCATTGTCCAAGGAGTCAGATTTGCTTTCCGTGTACATCAGTTTGCTAGCGGTTTTGATGCGGATACAATGAGAGCATTCCAAGAATTGAGAGATAAAGCCAGGTCATGTCATGTTCAATGCCATGGCCAGCAACAAAAATTCTTGTGCAGGCCTCCAGCATGTTAA
Protein sequence

>Msa0652400

VDERAVLKHFDWPEQKADAMREAAFGYCDLKKLESEASSFRDDPRQLCSPALKKMQTLFEKLEHGVYNISRMRESATKRFKVFQIPVDWLLDSGYATKIKLASVKLAMKYMKRVSAELETVGGGPEEEELIVQGVRFAFRVHQFASGFDADTMRAFQELRDKARSCHVQCHGQQQKFLCRPPAC*