AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0788960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0788960 MtrunA17_Chr4g0061171 100.000 160 0 0 1 160 1 160 2.83e-118 330
Msa0788960 MtrunA17_Chr3g0091481 96.250 160 6 0 1 160 1 160 5.53e-113 317
Msa0788960 MtrunA17_Chr4g0074021 93.711 159 10 0 1 159 1 159 2.73e-109 308
Msa0788960 MtrunA17_Chr5g0418211 91.824 159 13 0 1 159 1 159 9.03e-108 304
Msa0788960 MtrunA17_Chr4g0062241 71.569 102 19 1 1 102 1 92 4.95e-48 150
Msa0788960 MtrunA17_Chr4g0074011 46.094 128 21 2 18 145 42 121 1.23e-25 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0788960 AT1G13950.1 91.195 159 13 1 1 159 1 158 7.23e-104 294
Msa0788960 AT1G69410.1 86.792 159 20 1 1 159 1 158 5.64e-101 287
Msa0788960 AT1G26630.1 81.646 158 28 1 1 158 1 157 3.47e-95 272
Msa0788960 AT1G26630.2 83.465 127 20 1 1 127 1 126 4.40e-76 223

Find 71 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAATGAACGCTAAAATTT+TGG 0.101895 5_2:+76628701 None:intergenic
AGTGGTGTTGGTGGTGTTTA+TGG 0.113621 5_2:-76626887 Msa0788960:three_prime_UTR
TCAAACGAGTATTTCCTTAT+TGG 0.168992 5_2:-76626815 Msa0788960:three_prime_UTR
GTGAAGATAAGTGGTAAATT+AGG 0.184677 5_2:+76628638 None:intergenic
CATTACATTTGGCAAATTAA+TGG 0.194602 5_2:+76626535 None:intergenic
CAAACGAGTATTTCCTTATT+GGG 0.252830 5_2:-76626814 Msa0788960:three_prime_UTR
TTCGTCAGACATATTGATTC+AGG 0.274394 5_2:+76628549 None:intergenic
ACTCATTTCATTCCATAGTT+TGG 0.285322 5_2:-76626606 Msa0788960:three_prime_UTR
CATCGGCCTTAGATTCAAAT+TGG 0.297056 5_2:+76628521 None:intergenic
CAATTTCCCCAACCAAACTA+TGG 0.307891 5_2:+76626594 None:intergenic
TGAAGATAAGTGGTAAATTA+GGG 0.331085 5_2:+76628639 None:intergenic
GTGAGCTTGCTCACTGAAAA+TGG 0.342511 5_2:-76627289 Msa0788960:CDS
TAAAGGACAAATACCCAATA+AGG 0.344877 5_2:+76626801 None:intergenic
CTTATTGACATTTCTGAAGA+TGG 0.348595 5_2:-76628025 Msa0788960:CDS
CTTGGCATGTCCATGCTTCC+CGG 0.359341 5_2:+76628282 None:intergenic
ATCTGTGCTTTGAAGGATAT+TGG 0.363464 5_2:-76627020 Msa0788960:CDS
TTTCATTCCATAGTTTGGTT+GGG 0.375187 5_2:-76626601 Msa0788960:three_prime_UTR
AGAGCACCAATTTGAATCTA+AGG 0.377994 5_2:-76628527 Msa0788960:CDS
CGATAATGAGAACAGAGATT+TGG 0.380861 5_2:+76628596 None:intergenic
AGCTTGTTGAGGATAAGTTT+TGG 0.388597 5_2:+76628489 None:intergenic
TACTATACTTGTAAAGATAA+AGG 0.392946 5_2:+76626784 None:intergenic
CGTAAAACTCTGCTCTAGTC+TGG 0.417524 5_2:+76626711 None:intergenic
CTGTTCTCATTATCGCTCTT+CGG 0.419231 5_2:-76628588 Msa0788960:five_prime_UTR
AATGTCAATAAGCTGATAAT+CGG 0.422180 5_2:+76628036 None:intergenic
ATTTCATTCCATAGTTTGGT+TGG 0.427612 5_2:-76626602 Msa0788960:three_prime_UTR
TTATAATATTCGCAGACCTC+AGG 0.458657 5_2:+76628676 None:intergenic
TTCATTCCATAGTTTGGTTG+GGG 0.466367 5_2:-76626600 Msa0788960:three_prime_UTR
GGAGCAGATCTGTGCTTTGA+AGG 0.467522 5_2:-76627027 Msa0788960:CDS
ATGAGAACAGAGATTTGGTT+CGG 0.473998 5_2:+76628601 None:intergenic
TAACTATGTATCCATTCTTA+CGG 0.477130 5_2:+76628458 None:intergenic
GCTGGTACCATCCGTAAGAA+TGG 0.484451 5_2:-76628469 Msa0788960:CDS
TGATTCAGGAGTGGCGGTGG+CGG 0.509670 5_2:+76628563 None:intergenic
TGTATCCGTGATGTCTGCTA+TGG 0.510337 5_2:-76627054 Msa0788960:CDS
CTTTGTGTGCAGATCAAGGA+TGG 0.512763 5_2:-76627101 Msa0788960:intron
CACTGAAAATGGAAACACTA+AGG 0.519818 5_2:-76627278 Msa0788960:CDS
TGTGAGACTAGTGGTGTTGG+TGG 0.526439 5_2:-76626896 Msa0788960:three_prime_UTR
GAAGTTTCGACTTCGAAGAC+CGG 0.527896 5_2:-76628301 Msa0788960:CDS
GAATAATCACCTTGCATGGA+CGG 0.531922 5_2:+76628428 None:intergenic
GGATGGATTTGCTGAGGGAA+AGG 0.534900 5_2:-76627084 Msa0788960:CDS
TGTCATCGGTGGGAAGTTTG+AGG 0.539905 5_2:+76627251 None:intergenic
CAATTGAAAGTGAAGATAAG+TGG 0.541516 5_2:+76628629 None:intergenic
ACTTCGAAGACCGGGAAGCA+TGG 0.555740 5_2:-76628292 Msa0788960:CDS
CTGAGTGAGCAGACTGTCAT+CGG 0.555770 5_2:+76627237 None:intergenic
TATTGATTCAGGAGTGGCGG+TGG 0.557198 5_2:+76628560 None:intergenic
TTTGAATCTAAGGCCGATGC+CGG 0.559985 5_2:-76628517 Msa0788960:CDS
TTACGAATAATCACCTTGCA+TGG 0.560163 5_2:+76628424 None:intergenic
TCTTGTGAGACTAGTGGTGT+TGG 0.565302 5_2:-76626899 Msa0788960:three_prime_UTR
AAAACTTATCCTCAACAAGC+TGG 0.581192 5_2:-76628487 Msa0788960:CDS
TAGCGTTCATTCCACGCCTG+AGG 0.590265 5_2:-76628692 Msa0788960:five_prime_UTR
AGTGAGCAGACTGTCATCGG+TGG 0.592770 5_2:+76627240 None:intergenic
AAGTTTCGACTTCGAAGACC+GGG 0.594274 5_2:-76628300 Msa0788960:CDS
TGTGCTTTGAAGGATATTGG+TGG 0.594489 5_2:-76627017 Msa0788960:CDS
CGGATGGTACCAGCTTGTTG+AGG 0.595998 5_2:+76628478 None:intergenic
CGTGATGTCTGCTATGGGTG+AGG 0.597852 5_2:-76627048 Msa0788960:CDS
CGATGACAGTCTGCTCACTC+AGG 0.597987 5_2:-76627236 Msa0788960:intron
ATAGATTCTTGTGAGACTAG+TGG 0.603554 5_2:-76626905 Msa0788960:three_prime_UTR
TATGTATCCATTCTTACGGA+TGG 0.607030 5_2:+76628462 None:intergenic
ATTCACTTCAAAGAAGCTTG+AGG 0.607930 5_2:-76628236 Msa0788960:CDS
ACATCACAATTATGTGAAGA+CGG 0.610965 5_2:+76628205 None:intergenic
GTATCCGTGATGTCTGCTAT+GGG 0.612684 5_2:-76627053 Msa0788960:CDS
GTGAGCAGACTGTCATCGGT+GGG 0.615967 5_2:+76627241 None:intergenic
GATCAAGGATGGATTTGCTG+AGG 0.618132 5_2:-76627090 Msa0788960:CDS
ATCAAGGATGGATTTGCTGA+GGG 0.621184 5_2:-76627089 Msa0788960:CDS
AATAGCAACAAAATGACACT+TGG 0.628478 5_2:+76628264 None:intergenic
TTCATGCAATACATAAACTG+TGG 0.634184 5_2:+76626561 None:intergenic
CAGACATCACGGATACAACA+AGG 0.636222 5_2:+76627060 None:intergenic
ACATATTGATTCAGGAGTGG+CGG 0.644758 5_2:+76628557 None:intergenic
CTCACCCATAGCAGACATCA+CGG 0.653023 5_2:+76627049 None:intergenic
ATATTCGCAGACCTCAGGCG+TGG 0.676343 5_2:+76628681 None:intergenic
CAGACATATTGATTCAGGAG+TGG 0.678905 5_2:+76628554 None:intergenic
TAATCGGTACGATTCACGTG+AGG 0.748646 5_2:+76628052 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAACAAACTAAATTAAAA+CGG - chr5_2:76627787-76627806 Msa0788960:intron 10.0%
!! TTAAACAAACTAAATTAAAA+CGG - chr5_2:76627787-76627806 Msa0788960:intron 10.0%
!!! CAATTGTTTTTATTTTTGTT+AGG - chr5_2:76627166-76627185 Msa0788960:intron 15.0%
!! TAAACAAACTAAATTAAAAC+GGG - chr5_2:76627788-76627807 Msa0788960:intron 15.0%
!!! CAATTGTTTTTATTTTTGTT+AGG - chr5_2:76627166-76627185 Msa0788960:intron 15.0%
!! TAAACAAACTAAATTAAAAC+GGG - chr5_2:76627788-76627807 Msa0788960:intron 15.0%
!! CACTTAATTAATCAAATCAA+AGG + chr5_2:76627272-76627291 None:intergenic 20.0%
!!! GATTTTGATATTCAATTAGT+TGG - chr5_2:76627349-76627368 Msa0788960:intron 20.0%
!!! GATTTTTTTACTTTCAGATT+AGG - chr5_2:76627407-76627426 Msa0788960:intron 20.0%
!!! AGCTTAGAAAAAAAGATTTT+GGG + chr5_2:76627749-76627768 None:intergenic 20.0%
!!! TTTAGTTTGTTTAAGCTTTA+TGG + chr5_2:76627781-76627800 None:intergenic 20.0%
!! TTCATAATATAGCTATCAAA+AGG + chr5_2:76627876-76627895 None:intergenic 20.0%
!!! TGATTATGTTTTTGTATTGT+AGG - chr5_2:76627932-76627951 Msa0788960:intron 20.0%
!! TACTATACTTGTAAAGATAA+AGG + chr5_2:76628462-76628481 None:intergenic 20.0%
!! CACTTAATTAATCAAATCAA+AGG + chr5_2:76627272-76627291 None:intergenic 20.0%
!!! GATTTTGATATTCAATTAGT+TGG - chr5_2:76627349-76627368 Msa0788960:intron 20.0%
!!! GATTTTTTTACTTTCAGATT+AGG - chr5_2:76627407-76627426 Msa0788960:intron 20.0%
!!! AGCTTAGAAAAAAAGATTTT+GGG + chr5_2:76627749-76627768 None:intergenic 20.0%
!!! TTTAGTTTGTTTAAGCTTTA+TGG + chr5_2:76627781-76627800 None:intergenic 20.0%
!! TTCATAATATAGCTATCAAA+AGG + chr5_2:76627876-76627895 None:intergenic 20.0%
!!! TGATTATGTTTTTGTATTGT+AGG - chr5_2:76627932-76627951 Msa0788960:intron 20.0%
!! TACTATACTTGTAAAGATAA+AGG + chr5_2:76628462-76628481 None:intergenic 20.0%
! TGAAGATAAGTGGTAAATTA+GGG + chr5_2:76626607-76626626 None:intergenic 25.0%
! TAACTATGTATCCATTCTTA+CGG + chr5_2:76626788-76626807 None:intergenic 25.0%
!! TTTTTGTACTTCGTTATTCA+TGG - chr5_2:76626870-76626889 Msa0788960:three_prime_UTR 25.0%
! AATGTCAATAAGCTGATAAT+CGG + chr5_2:76627210-76627229 None:intergenic 25.0%
! GTTCATACTTCTTTATAATC+TGG + chr5_2:76627296-76627315 None:intergenic 25.0%
! AAGTATGAACTGATTGTTAT+GGG - chr5_2:76627306-76627325 Msa0788960:intron 25.0%
!! TTAGTTGGAAATTTGTTTGA+AGG - chr5_2:76627364-76627383 Msa0788960:intron 25.0%
! TAATCTGATTTAGATTAGTG+TGG + chr5_2:76627690-76627709 None:intergenic 25.0%
!! GAGCTTAGAAAAAAAGATTT+TGG + chr5_2:76627750-76627769 None:intergenic 25.0%
! TGAAGATAAGTGGTAAATTA+GGG + chr5_2:76626607-76626626 None:intergenic 25.0%
! TAACTATGTATCCATTCTTA+CGG + chr5_2:76626788-76626807 None:intergenic 25.0%
!! TTTTTGTACTTCGTTATTCA+TGG - chr5_2:76626870-76626889 Msa0788960:three_prime_UTR 25.0%
! AATGTCAATAAGCTGATAAT+CGG + chr5_2:76627210-76627229 None:intergenic 25.0%
! GTTCATACTTCTTTATAATC+TGG + chr5_2:76627296-76627315 None:intergenic 25.0%
! AAGTATGAACTGATTGTTAT+GGG - chr5_2:76627306-76627325 Msa0788960:intron 25.0%
!! TTAGTTGGAAATTTGTTTGA+AGG - chr5_2:76627364-76627383 Msa0788960:intron 25.0%
! TAATCTGATTTAGATTAGTG+TGG + chr5_2:76627690-76627709 None:intergenic 25.0%
!! GAGCTTAGAAAAAAAGATTT+TGG + chr5_2:76627750-76627769 None:intergenic 25.0%
GTGAAGATAAGTGGTAAATT+AGG + chr5_2:76626608-76626627 None:intergenic 30.0%
CAATTGAAAGTGAAGATAAG+TGG + chr5_2:76626617-76626636 None:intergenic 30.0%
!!! TGTTTTTTGTTGTTGCGATT+AGG - chr5_2:76626914-76626933 Msa0788960:three_prime_UTR 30.0%
AATAGCAACAAAATGACACT+TGG + chr5_2:76626982-76627001 None:intergenic 30.0%
ACATCACAATTATGTGAAGA+CGG + chr5_2:76627041-76627060 None:intergenic 30.0%
! CTTATTGACATTTCTGAAGA+TGG - chr5_2:76627218-76627237 Msa0788960:intron 30.0%
GAAGTATGAACTGATTGTTA+TGG - chr5_2:76627305-76627324 Msa0788960:intron 30.0%
! GGTACAATAACTTTTGTAAC+AGG + chr5_2:76627825-76627844 None:intergenic 30.0%
TAAGCCTGTTACAGATTAAT+GGG + chr5_2:76627847-76627866 None:intergenic 30.0%
ATAAGCCTGTTACAGATTAA+TGG + chr5_2:76627848-76627867 None:intergenic 30.0%
! GATTTGTTCTTGCATTGTAA+TGG - chr5_2:76628038-76628057 Msa0788960:CDS 30.0%
! TGGTGTTTATGGATTTTTGT+TGG - chr5_2:76628367-76628386 Msa0788960:intron 30.0%
TCAAACGAGTATTTCCTTAT+TGG - chr5_2:76628428-76628447 Msa0788960:intron 30.0%
CAAACGAGTATTTCCTTATT+GGG - chr5_2:76628429-76628448 Msa0788960:intron 30.0%
TAAAGGACAAATACCCAATA+AGG + chr5_2:76628445-76628464 None:intergenic 30.0%
ACTCATTTCATTCCATAGTT+TGG - chr5_2:76628637-76628656 Msa0788960:five_prime_UTR 30.0%
ATTTCATTCCATAGTTTGGT+TGG - chr5_2:76628641-76628660 Msa0788960:five_prime_UTR 30.0%
TTTCATTCCATAGTTTGGTT+GGG - chr5_2:76628642-76628661 Msa0788960:five_prime_UTR 30.0%
TTCATGCAATACATAAACTG+TGG + chr5_2:76628685-76628704 None:intergenic 30.0%
! GGAATGAACGCTAAAATTTT+TGG + chr5_2:76626544-76626563 None:intergenic 30.0%
GTGAAGATAAGTGGTAAATT+AGG + chr5_2:76626608-76626627 None:intergenic 30.0%
CAATTGAAAGTGAAGATAAG+TGG + chr5_2:76626617-76626636 None:intergenic 30.0%
!!! TGTTTTTTGTTGTTGCGATT+AGG - chr5_2:76626914-76626933 Msa0788960:three_prime_UTR 30.0%
AATAGCAACAAAATGACACT+TGG + chr5_2:76626982-76627001 None:intergenic 30.0%
ACATCACAATTATGTGAAGA+CGG + chr5_2:76627041-76627060 None:intergenic 30.0%
! CTTATTGACATTTCTGAAGA+TGG - chr5_2:76627218-76627237 Msa0788960:intron 30.0%
GAAGTATGAACTGATTGTTA+TGG - chr5_2:76627305-76627324 Msa0788960:intron 30.0%
! GGTACAATAACTTTTGTAAC+AGG + chr5_2:76627825-76627844 None:intergenic 30.0%
TAAGCCTGTTACAGATTAAT+GGG + chr5_2:76627847-76627866 None:intergenic 30.0%
ATAAGCCTGTTACAGATTAA+TGG + chr5_2:76627848-76627867 None:intergenic 30.0%
! GATTTGTTCTTGCATTGTAA+TGG - chr5_2:76628038-76628057 Msa0788960:CDS 30.0%
! TGGTGTTTATGGATTTTTGT+TGG - chr5_2:76628367-76628386 Msa0788960:intron 30.0%
TCAAACGAGTATTTCCTTAT+TGG - chr5_2:76628428-76628447 Msa0788960:intron 30.0%
CAAACGAGTATTTCCTTATT+GGG - chr5_2:76628429-76628448 Msa0788960:intron 30.0%
TAAAGGACAAATACCCAATA+AGG + chr5_2:76628445-76628464 None:intergenic 30.0%
ACTCATTTCATTCCATAGTT+TGG - chr5_2:76628637-76628656 Msa0788960:five_prime_UTR 30.0%
ATTTCATTCCATAGTTTGGT+TGG - chr5_2:76628641-76628660 Msa0788960:five_prime_UTR 30.0%
TTTCATTCCATAGTTTGGTT+GGG - chr5_2:76628642-76628661 Msa0788960:five_prime_UTR 30.0%
TTCATGCAATACATAAACTG+TGG + chr5_2:76628685-76628704 None:intergenic 30.0%
TTATAATATTCGCAGACCTC+AGG + chr5_2:76626570-76626589 None:intergenic 35.0%
! ATGAGAACAGAGATTTGGTT+CGG + chr5_2:76626645-76626664 None:intergenic 35.0%
CGATAATGAGAACAGAGATT+TGG + chr5_2:76626650-76626669 None:intergenic 35.0%
!! TTCGTCAGACATATTGATTC+AGG + chr5_2:76626697-76626716 None:intergenic 35.0%
! AGAGCACCAATTTGAATCTA+AGG - chr5_2:76626716-76626735 Msa0788960:three_prime_UTR 35.0%
!!! AGCTTGTTGAGGATAAGTTT+TGG + chr5_2:76626757-76626776 None:intergenic 35.0%
AAAACTTATCCTCAACAAGC+TGG - chr5_2:76626756-76626775 Msa0788960:three_prime_UTR 35.0%
TATGTATCCATTCTTACGGA+TGG + chr5_2:76626784-76626803 None:intergenic 35.0%
TTACGAATAATCACCTTGCA+TGG + chr5_2:76626822-76626841 None:intergenic 35.0%
!!! TTTTTGTTGTTGCGATTAGG+TGG - chr5_2:76626917-76626936 Msa0788960:three_prime_UTR 35.0%
ATTCACTTCAAAGAAGCTTG+AGG - chr5_2:76627007-76627026 Msa0788960:CDS 35.0%
! GTTGGAAATTTGTTTGAAGG+AGG - chr5_2:76627367-76627386 Msa0788960:intron 35.0%
! TTCAGACCCTTTTATGTTGA+AGG - chr5_2:76627609-76627628 Msa0788960:intron 35.0%
! TCAGACCCTTTTATGTTGAA+GGG - chr5_2:76627610-76627629 Msa0788960:intron 35.0%
CCATACCCTTCAACATAAAA+GGG + chr5_2:76627618-76627637 None:intergenic 35.0%
CATACACAAAATCAACTCAC+CGG + chr5_2:76627641-76627660 None:intergenic 35.0%
AAGCCTGTTACAGATTAATG+GGG + chr5_2:76627846-76627865 None:intergenic 35.0%
CACTGAAAATGGAAACACTA+AGG - chr5_2:76627965-76627984 Msa0788960:intron 35.0%
!! ATCTGTGCTTTGAAGGATAT+TGG - chr5_2:76628223-76628242 Msa0788960:CDS 35.0%
ATAGATTCTTGTGAGACTAG+TGG - chr5_2:76628338-76628357 Msa0788960:intron 35.0%
TTCATTCCATAGTTTGGTTG+GGG - chr5_2:76628643-76628662 Msa0788960:five_prime_UTR 35.0%
TTATAATATTCGCAGACCTC+AGG + chr5_2:76626570-76626589 None:intergenic 35.0%
! ATGAGAACAGAGATTTGGTT+CGG + chr5_2:76626645-76626664 None:intergenic 35.0%
CGATAATGAGAACAGAGATT+TGG + chr5_2:76626650-76626669 None:intergenic 35.0%
!! TTCGTCAGACATATTGATTC+AGG + chr5_2:76626697-76626716 None:intergenic 35.0%
! AGAGCACCAATTTGAATCTA+AGG - chr5_2:76626716-76626735 Msa0788960:three_prime_UTR 35.0%
!!! AGCTTGTTGAGGATAAGTTT+TGG + chr5_2:76626757-76626776 None:intergenic 35.0%
AAAACTTATCCTCAACAAGC+TGG - chr5_2:76626756-76626775 Msa0788960:three_prime_UTR 35.0%
TATGTATCCATTCTTACGGA+TGG + chr5_2:76626784-76626803 None:intergenic 35.0%
TTACGAATAATCACCTTGCA+TGG + chr5_2:76626822-76626841 None:intergenic 35.0%
!!! TTTTTGTTGTTGCGATTAGG+TGG - chr5_2:76626917-76626936 Msa0788960:three_prime_UTR 35.0%
ATTCACTTCAAAGAAGCTTG+AGG - chr5_2:76627007-76627026 Msa0788960:CDS 35.0%
! GTTGGAAATTTGTTTGAAGG+AGG - chr5_2:76627367-76627386 Msa0788960:intron 35.0%
! TTCAGACCCTTTTATGTTGA+AGG - chr5_2:76627609-76627628 Msa0788960:intron 35.0%
! TCAGACCCTTTTATGTTGAA+GGG - chr5_2:76627610-76627629 Msa0788960:intron 35.0%
CCATACCCTTCAACATAAAA+GGG + chr5_2:76627618-76627637 None:intergenic 35.0%
CATACACAAAATCAACTCAC+CGG + chr5_2:76627641-76627660 None:intergenic 35.0%
AAGCCTGTTACAGATTAATG+GGG + chr5_2:76627846-76627865 None:intergenic 35.0%
CACTGAAAATGGAAACACTA+AGG - chr5_2:76627965-76627984 Msa0788960:intron 35.0%
!! ATCTGTGCTTTGAAGGATAT+TGG - chr5_2:76628223-76628242 Msa0788960:CDS 35.0%
ATAGATTCTTGTGAGACTAG+TGG - chr5_2:76628338-76628357 Msa0788960:intron 35.0%
TTCATTCCATAGTTTGGTTG+GGG - chr5_2:76628643-76628662 Msa0788960:five_prime_UTR 35.0%
CTGTTCTCATTATCGCTCTT+CGG - chr5_2:76626655-76626674 Msa0788960:three_prime_UTR 40.0%
!! ACATATTGATTCAGGAGTGG+CGG + chr5_2:76626689-76626708 None:intergenic 40.0%
!! CAGACATATTGATTCAGGAG+TGG + chr5_2:76626692-76626711 None:intergenic 40.0%
CATCGGCCTTAGATTCAAAT+TGG + chr5_2:76626725-76626744 None:intergenic 40.0%
!!! AGGATAAGTTTTGGAAGCAC+CGG + chr5_2:76626748-76626767 None:intergenic 40.0%
TATCAAAAACCGTCCATGCA+AGG - chr5_2:76626806-76626825 Msa0788960:three_prime_UTR 40.0%
GAATAATCACCTTGCATGGA+CGG + chr5_2:76626818-76626837 None:intergenic 40.0%
! CCCTTTTATGTTGAAGGGTA+TGG - chr5_2:76627615-76627634 Msa0788960:intron 40.0%
GCCATACCCTTCAACATAAA+AGG + chr5_2:76627619-76627638 None:intergenic 40.0%
! TTTATGTTGAAGGGTATGGC+CGG - chr5_2:76627619-76627638 Msa0788960:intron 40.0%
GTACCCCATTAATCTGTAAC+AGG - chr5_2:76627840-76627859 Msa0788960:intron 40.0%
TTTGCTTTGTGTGCAGATCA+AGG - chr5_2:76628138-76628157 Msa0788960:intron 40.0%
!! ATCAAGGATGGATTTGCTGA+GGG - chr5_2:76628154-76628173 Msa0788960:intron 40.0%
!! TGTGCTTTGAAGGATATTGG+TGG - chr5_2:76628226-76628245 Msa0788960:CDS 40.0%
CAATTTCCCCAACCAAACTA+TGG + chr5_2:76628652-76628671 None:intergenic 40.0%
CTGTTCTCATTATCGCTCTT+CGG - chr5_2:76626655-76626674 Msa0788960:three_prime_UTR 40.0%
!! ACATATTGATTCAGGAGTGG+CGG + chr5_2:76626689-76626708 None:intergenic 40.0%
!! CAGACATATTGATTCAGGAG+TGG + chr5_2:76626692-76626711 None:intergenic 40.0%
CATCGGCCTTAGATTCAAAT+TGG + chr5_2:76626725-76626744 None:intergenic 40.0%
!!! AGGATAAGTTTTGGAAGCAC+CGG + chr5_2:76626748-76626767 None:intergenic 40.0%
TATCAAAAACCGTCCATGCA+AGG - chr5_2:76626806-76626825 Msa0788960:three_prime_UTR 40.0%
GAATAATCACCTTGCATGGA+CGG + chr5_2:76626818-76626837 None:intergenic 40.0%
! CCCTTTTATGTTGAAGGGTA+TGG - chr5_2:76627615-76627634 Msa0788960:intron 40.0%
GCCATACCCTTCAACATAAA+AGG + chr5_2:76627619-76627638 None:intergenic 40.0%
! TTTATGTTGAAGGGTATGGC+CGG - chr5_2:76627619-76627638 Msa0788960:intron 40.0%
GTACCCCATTAATCTGTAAC+AGG - chr5_2:76627840-76627859 Msa0788960:intron 40.0%
TTTGCTTTGTGTGCAGATCA+AGG - chr5_2:76628138-76628157 Msa0788960:intron 40.0%
!! ATCAAGGATGGATTTGCTGA+GGG - chr5_2:76628154-76628173 Msa0788960:intron 40.0%
!! TGTGCTTTGAAGGATATTGG+TGG - chr5_2:76628226-76628245 Msa0788960:CDS 40.0%
CAATTTCCCCAACCAAACTA+TGG + chr5_2:76628652-76628671 None:intergenic 40.0%
! TTTGAATCTAAGGCCGATGC+CGG - chr5_2:76626726-76626745 Msa0788960:three_prime_UTR 45.0%
GAAGTTTCGACTTCGAAGAC+CGG - chr5_2:76626942-76626961 Msa0788960:three_prime_UTR 45.0%
AAGTTTCGACTTCGAAGACC+GGG - chr5_2:76626943-76626962 Msa0788960:three_prime_UTR 45.0%
TAATCGGTACGATTCACGTG+AGG + chr5_2:76627194-76627213 None:intergenic 45.0%
! GTGAGCTTGCTCACTGAAAA+TGG - chr5_2:76627954-76627973 Msa0788960:intron 45.0%
CTTTGTGTGCAGATCAAGGA+TGG - chr5_2:76628142-76628161 Msa0788960:intron 45.0%
!! GATCAAGGATGGATTTGCTG+AGG - chr5_2:76628153-76628172 Msa0788960:intron 45.0%
CAGACATCACGGATACAACA+AGG + chr5_2:76628186-76628205 None:intergenic 45.0%
TGTATCCGTGATGTCTGCTA+TGG - chr5_2:76628189-76628208 Msa0788960:intron 45.0%
GTATCCGTGATGTCTGCTAT+GGG - chr5_2:76628190-76628209 Msa0788960:intron 45.0%
TCTTGTGAGACTAGTGGTGT+TGG - chr5_2:76628344-76628363 Msa0788960:intron 45.0%
!! AGTGGTGTTGGTGGTGTTTA+TGG - chr5_2:76628356-76628375 Msa0788960:intron 45.0%
CGTAAAACTCTGCTCTAGTC+TGG + chr5_2:76628535-76628554 None:intergenic 45.0%
! TTTGAATCTAAGGCCGATGC+CGG - chr5_2:76626726-76626745 Msa0788960:three_prime_UTR 45.0%
GAAGTTTCGACTTCGAAGAC+CGG - chr5_2:76626942-76626961 Msa0788960:three_prime_UTR 45.0%
AAGTTTCGACTTCGAAGACC+GGG - chr5_2:76626943-76626962 Msa0788960:three_prime_UTR 45.0%
TAATCGGTACGATTCACGTG+AGG + chr5_2:76627194-76627213 None:intergenic 45.0%
! GTGAGCTTGCTCACTGAAAA+TGG - chr5_2:76627954-76627973 Msa0788960:intron 45.0%
CTTTGTGTGCAGATCAAGGA+TGG - chr5_2:76628142-76628161 Msa0788960:intron 45.0%
!! GATCAAGGATGGATTTGCTG+AGG - chr5_2:76628153-76628172 Msa0788960:intron 45.0%
CAGACATCACGGATACAACA+AGG + chr5_2:76628186-76628205 None:intergenic 45.0%
TGTATCCGTGATGTCTGCTA+TGG - chr5_2:76628189-76628208 Msa0788960:intron 45.0%
GTATCCGTGATGTCTGCTAT+GGG - chr5_2:76628190-76628209 Msa0788960:intron 45.0%
TCTTGTGAGACTAGTGGTGT+TGG - chr5_2:76628344-76628363 Msa0788960:intron 45.0%
!! AGTGGTGTTGGTGGTGTTTA+TGG - chr5_2:76628356-76628375 Msa0788960:intron 45.0%
CGTAAAACTCTGCTCTAGTC+TGG + chr5_2:76628535-76628554 None:intergenic 45.0%
!! TATTGATTCAGGAGTGGCGG+TGG + chr5_2:76626686-76626705 None:intergenic 50.0%
!!! AGTTTTGGAAGCACCGGCAT+CGG + chr5_2:76626742-76626761 None:intergenic 50.0%
GCTGGTACCATCCGTAAGAA+TGG - chr5_2:76626774-76626793 Msa0788960:three_prime_UTR 50.0%
TGTCATCGGTGGGAAGTTTG+AGG + chr5_2:76627995-76628014 None:intergenic 50.0%
CTGAGTGAGCAGACTGTCAT+CGG + chr5_2:76628009-76628028 None:intergenic 50.0%
!! GGATGGATTTGCTGAGGGAA+AGG - chr5_2:76628159-76628178 Msa0788960:intron 50.0%
CTCACCCATAGCAGACATCA+CGG + chr5_2:76628197-76628216 None:intergenic 50.0%
GGAGCAGATCTGTGCTTTGA+AGG - chr5_2:76628216-76628235 Msa0788960:CDS 50.0%
! TGTGAGACTAGTGGTGTTGG+TGG - chr5_2:76628347-76628366 Msa0788960:intron 50.0%
!! TATTGATTCAGGAGTGGCGG+TGG + chr5_2:76626686-76626705 None:intergenic 50.0%
!!! AGTTTTGGAAGCACCGGCAT+CGG + chr5_2:76626742-76626761 None:intergenic 50.0%
GCTGGTACCATCCGTAAGAA+TGG - chr5_2:76626774-76626793 Msa0788960:three_prime_UTR 50.0%
TGTCATCGGTGGGAAGTTTG+AGG + chr5_2:76627995-76628014 None:intergenic 50.0%
CTGAGTGAGCAGACTGTCAT+CGG + chr5_2:76628009-76628028 None:intergenic 50.0%
!! GGATGGATTTGCTGAGGGAA+AGG - chr5_2:76628159-76628178 Msa0788960:intron 50.0%
CTCACCCATAGCAGACATCA+CGG + chr5_2:76628197-76628216 None:intergenic 50.0%
GGAGCAGATCTGTGCTTTGA+AGG - chr5_2:76628216-76628235 Msa0788960:CDS 50.0%
! TGTGAGACTAGTGGTGTTGG+TGG - chr5_2:76628347-76628366 Msa0788960:intron 50.0%
TAGCGTTCATTCCACGCCTG+AGG - chr5_2:76626551-76626570 Msa0788960:three_prime_UTR 55.0%
ATATTCGCAGACCTCAGGCG+TGG + chr5_2:76626565-76626584 None:intergenic 55.0%
!! CGGATGGTACCAGCTTGTTG+AGG + chr5_2:76626768-76626787 None:intergenic 55.0%
ACTTCGAAGACCGGGAAGCA+TGG - chr5_2:76626951-76626970 Msa0788960:three_prime_UTR 55.0%
CTTGGCATGTCCATGCTTCC+CGG + chr5_2:76626964-76626983 None:intergenic 55.0%
GTGAGCAGACTGTCATCGGT+GGG + chr5_2:76628005-76628024 None:intergenic 55.0%
AGTGAGCAGACTGTCATCGG+TGG + chr5_2:76628006-76628025 None:intergenic 55.0%
CGATGACAGTCTGCTCACTC+AGG - chr5_2:76628007-76628026 Msa0788960:intron 55.0%
CGTGATGTCTGCTATGGGTG+AGG - chr5_2:76628195-76628214 Msa0788960:intron 55.0%
TAGCGTTCATTCCACGCCTG+AGG - chr5_2:76626551-76626570 Msa0788960:three_prime_UTR 55.0%
ATATTCGCAGACCTCAGGCG+TGG + chr5_2:76626565-76626584 None:intergenic 55.0%
!! CGGATGGTACCAGCTTGTTG+AGG + chr5_2:76626768-76626787 None:intergenic 55.0%
ACTTCGAAGACCGGGAAGCA+TGG - chr5_2:76626951-76626970 Msa0788960:three_prime_UTR 55.0%
CTTGGCATGTCCATGCTTCC+CGG + chr5_2:76626964-76626983 None:intergenic 55.0%
GTGAGCAGACTGTCATCGGT+GGG + chr5_2:76628005-76628024 None:intergenic 55.0%
AGTGAGCAGACTGTCATCGG+TGG + chr5_2:76628006-76628025 None:intergenic 55.0%
CGATGACAGTCTGCTCACTC+AGG - chr5_2:76628007-76628026 Msa0788960:intron 55.0%
CGTGATGTCTGCTATGGGTG+AGG - chr5_2:76628195-76628214 Msa0788960:intron 55.0%
TGATTCAGGAGTGGCGGTGG+CGG + chr5_2:76626683-76626702 None:intergenic 60.0%
TGATTCAGGAGTGGCGGTGG+CGG + chr5_2:76626683-76626702 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 76626544 76628721 76626544 ID=Msa0788960;Name=Msa0788960
chr5_2 mRNA 76626544 76628721 76626544 ID=Msa0788960-mRNA-1;Parent=Msa0788960;Name=Msa0788960-mRNA-1;_AED=0.14;_eAED=0.14;_QI=161|1|1|1|1|1|5|463|160
chr5_2 exon 76626544 76627111 76626544 ID=Msa0788960-mRNA-1:exon:23321;Parent=Msa0788960-mRNA-1
chr5_2 exon 76627237 76627311 76627237 ID=Msa0788960-mRNA-1:exon:23320;Parent=Msa0788960-mRNA-1
chr5_2 exon 76628021 76628077 76628021 ID=Msa0788960-mRNA-1:exon:23319;Parent=Msa0788960-mRNA-1
chr5_2 exon 76628207 76628329 76628207 ID=Msa0788960-mRNA-1:exon:23318;Parent=Msa0788960-mRNA-1
chr5_2 exon 76628438 76628721 76628438 ID=Msa0788960-mRNA-1:exon:23317;Parent=Msa0788960-mRNA-1
chr5_2 five_prime_UTR 76628561 76628721 76628561 ID=Msa0788960-mRNA-1:five_prime_utr;Parent=Msa0788960-mRNA-1
chr5_2 CDS 76628438 76628560 76628438 ID=Msa0788960-mRNA-1:cds;Parent=Msa0788960-mRNA-1
chr5_2 CDS 76628207 76628329 76628207 ID=Msa0788960-mRNA-1:cds;Parent=Msa0788960-mRNA-1
chr5_2 CDS 76628021 76628077 76628021 ID=Msa0788960-mRNA-1:cds;Parent=Msa0788960-mRNA-1
chr5_2 CDS 76627237 76627311 76627237 ID=Msa0788960-mRNA-1:cds;Parent=Msa0788960-mRNA-1
chr5_2 CDS 76627007 76627111 76627007 ID=Msa0788960-mRNA-1:cds;Parent=Msa0788960-mRNA-1
chr5_2 three_prime_UTR 76626544 76627006 76626544 ID=Msa0788960-mRNA-1:three_prime_utr;Parent=Msa0788960-mRNA-1
Gene Sequence

>Msa0788960

ATGTCTGACGAAGAGCACCAATTTGAATCTAAGGCCGATGCCGGTGCTTCCAAAACTTATCCTCAACAAGCTGGTACCATCCGTAAGAATGGATACATAGTTATCAAAAACCGTCCATGCAAGGTGGTTGAAGTTTCGACTTCGAAGACCGGGAAGCATGGACATGCCAAGTGTCATTTTGTTGCTATTGATATATTCACTTCAAAGAAGCTTGAGGATATCGTGCCGTCTTCACATAATTGTGATGTTCCTCACGTGAATCGTACCGATTATCAGCTTATTGACATTTCTGAAGATGGATTTGTGAGCTTGCTCACTGAAAATGGAAACACTAAGGATGACCTCAAACTTCCCACCGATGACAGTCTGCTCACTCAGATCAAGGATGGATTTGCTGAGGGAAAGGACCTTGTTGTATCCGTGATGTCTGCTATGGGTGAGGAGCAGATCTGTGCTTTGAAGGATATTGGTGGAAAGAACTAA
Protein sequence

>Msa0788960

MSDEEHQFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDSLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGGKN*